FastQCFastQC Report
Fri 27 May 2016
SRR939029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003647
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117790.39215660162462496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.31222044401356086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81460.27120364010817516No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA79820.2657436110168738No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA68200.22705730733338503No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57600.1917668754018032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48560.16167012967902022No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44700.14881908559827436No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT41450.1379989059966101No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40280.13410364134001101No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34420.11459402519670255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT7200.026.3641413
GTACATG140050.023.4010661
CTTTGCG8200.022.5702762
TACATGG143200.022.4352932
ACATGGG143200.021.5736733
GAGTACT95900.021.37864712-13
GTACTTT102950.019.68414514-15
TTGCGTT9200.019.6011664
AGTACTT101700.019.38943312-13
CATGGGA91400.018.0164574
CATGGGG58450.017.4558124
GTAAGCG3500.016.9486694-95
ACTTTTT125150.016.30551716-17
GTATAGG7700.016.08471
TACTTTT123250.016.01848614-15
TACCTGG22850.015.1608232
CCGTCTA2205.7446514E-415.0997149
AGTACAT107300.015.0371442
GTGTAGC16450.014.768441
TAAGGTG9950.014.3081785
Produced by FastQC (version 0.11.5)