##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939029_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3003647 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23453288618803 33.0 31.0 34.0 31.0 34.0 2 32.46811126607088 34.0 31.0 34.0 31.0 34.0 3 32.558943843933726 34.0 31.0 34.0 31.0 34.0 4 36.08891590789464 37.0 35.0 37.0 35.0 37.0 5 36.01655454186194 37.0 35.0 37.0 35.0 37.0 6 36.05924131564062 37.0 35.0 37.0 35.0 37.0 7 35.99880578510058 37.0 35.0 37.0 35.0 37.0 8 35.97219280428093 37.0 35.0 37.0 35.0 37.0 9 37.65697067598156 39.0 37.0 39.0 35.0 39.0 10-11 37.572644355345346 39.0 37.0 39.0 35.0 39.0 12-13 37.487570942923725 39.0 37.0 39.0 35.0 39.0 14-15 38.800566111796755 40.0 38.0 41.0 34.5 41.0 16-17 38.893968565547155 40.0 38.0 41.0 35.0 41.0 18-19 38.94511438927411 40.0 38.0 41.0 35.0 41.0 20-21 38.91965766949312 40.0 38.0 41.0 35.0 41.0 22-23 38.84967574418698 40.0 38.0 41.0 34.5 41.0 24-25 38.7747298534082 40.0 38.0 41.0 34.0 41.0 26-27 38.61120397969535 40.0 38.0 41.0 34.0 41.0 28-29 38.457330205580085 40.0 38.0 41.0 34.0 41.0 30-31 38.283511011780014 40.0 38.0 41.0 34.0 41.0 32-33 38.20420442215746 40.0 38.0 41.0 34.0 41.0 34-35 38.1158708063897 40.0 38.0 41.0 33.0 41.0 36-37 37.98735354054588 40.0 37.5 41.0 33.0 41.0 38-39 37.841951800594416 40.0 37.0 41.0 33.0 41.0 40-41 37.67862352000751 40.0 37.0 41.0 33.0 41.0 42-43 37.50256821124453 40.0 37.0 41.0 32.5 41.0 44-45 37.25915045942483 40.0 37.0 41.0 31.5 41.0 46-47 36.94767710719668 40.0 36.0 41.0 31.0 41.0 48-49 36.61336784915138 39.5 36.0 41.0 30.5 41.0 50-51 36.35300569607547 39.0 36.0 41.0 30.0 41.0 52-53 36.087028535643505 39.0 35.0 40.5 29.5 41.0 54-55 35.79433152431028 39.0 35.0 40.0 28.5 41.0 56-57 35.665178531298785 38.5 35.0 40.0 28.0 41.0 58-59 35.39478873516096 38.0 35.0 40.0 28.0 41.0 60-61 35.02771863671064 38.0 34.0 40.0 27.5 41.0 62-63 34.613561114205496 37.5 34.0 40.0 26.5 41.0 64-65 34.19523848841092 37.0 33.5 39.5 26.0 41.0 66-67 33.74239100000766 36.0 33.0 39.0 26.0 40.5 68-69 33.259218043931256 36.0 32.5 39.0 24.5 40.0 70-71 32.735698635691875 35.0 32.0 38.0 23.5 40.0 72-73 32.2169697371229 35.0 31.5 37.0 22.0 39.5 74-75 31.706309196786442 35.0 31.0 37.0 20.5 39.0 76-77 29.803482899288767 33.0 28.5 35.0 18.5 37.0 78-79 30.757359137075696 34.0 30.5 36.0 19.5 38.0 80-81 30.749522830079567 34.0 31.0 35.5 18.5 37.0 82-83 30.349818404093426 34.0 30.0 35.0 17.5 37.0 84-85 29.913114790120144 34.0 30.0 35.0 10.5 36.0 86-87 29.238637230007388 34.0 29.0 35.0 4.5 36.0 88-89 28.554705995744506 33.5 29.0 35.0 2.0 35.0 90-91 27.98360343276024 33.0 27.0 35.0 2.0 35.0 92-93 27.60731287664629 33.0 27.0 35.0 2.0 35.0 94-95 27.07859362301895 33.0 26.0 34.0 2.0 35.0 96-97 26.249222528479546 32.0 25.0 34.0 2.0 35.0 98-99 25.083766501190055 32.0 20.5 34.0 2.0 35.0 100-101 19.582268322475976 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02441518108069829 1101 2 0.10754314198639037 1101 3 0.1821081029448095 1101 4 0.04431623625543324 1101 5 0.09622741277154745 1101 6 0.08476098795158293 1101 7 0.13262658890782575 1101 8 0.11551254804621891 1101 9 0.1711821262357418 1101 10-11 0.13761347454739337 1101 12-13 0.1808281426124907 1101 14-15 0.22613036895675975 1101 16-17 0.234040630915878 1101 18-19 0.21518796461352707 1101 20-21 0.23665070382976694 1101 22-23 0.24888698615527716 1101 24-25 0.13687469835649324 1101 26-27 0.14732665557565383 1101 28-29 0.11872165717997518 1101 30-31 0.03813204087736466 1101 32-33 0.16762740545407695 1101 34-35 0.1851976005686069 1101 36-37 0.1388755918531004 1101 38-39 0.17625413408736534 1101 40-41 0.13368022694469772 1101 42-43 0.112737648180115 1101 44-45 0.18245065431985807 1101 46-47 0.021552876829211698 1101 48-49 -0.1672209563247904 1101 50-51 -0.148250583405094 1101 52-53 -0.1988020140708926 1101 54-55 -0.13521789522062733 1101 56-57 -0.1741721324062908 1101 58-59 -0.14562157038864854 1101 60-61 -0.10771727765043693 1101 62-63 -0.1520795125507135 1101 64-65 -0.14423507227140675 1101 66-67 -0.1577201397857948 1101 68-69 -0.0012655649062622842 1101 70-71 -0.08205498227682995 1101 72-73 -0.08573221335905146 1101 74-75 -0.023356358800487698 1101 76-77 -0.4443253002915597 1101 78-79 0.0315261062503609 1101 80-81 -0.051625470630078496 1101 82-83 -0.16198740389385335 1101 84-85 -0.11484940197852467 1101 86-87 0.04982158576270379 1101 88-89 -0.01906717383970502 1101 90-91 -0.11992541456090677 1101 92-93 -0.25755469983208457 1101 94-95 -0.2864136797926733 1101 96-97 -0.15948595492155349 1101 98-99 0.013815583416597832 1101 100-101 0.45404924515864664 1102 1 0.13227720854390412 1102 2 0.12708797485471024 1102 3 0.16288385654183202 1102 4 0.09734758370332486 1102 5 0.0540112083839972 1102 6 0.07302785156556979 1102 7 0.12640633615877306 1102 8 0.06583641696683884 1102 9 0.12037628346534746 1102 10-11 0.18627231802898336 1102 12-13 0.21419347988477 1102 14-15 0.38296991160133587 1102 16-17 0.3108167127584309 1102 18-19 0.2148123970222997 1102 20-21 0.2008992951093802 1102 22-23 0.19537831500259273 1102 24-25 0.2205967291021551 1102 26-27 0.2696686874154679 1102 28-29 0.29072751089188387 1102 30-31 0.2647494707148894 1102 32-33 0.3090188929533966 1102 34-35 0.31549083323997706 1102 36-37 0.295868589281838 1102 38-39 0.3206851751727626 1102 40-41 0.36287985493309804 1102 42-43 0.2626695372331298 1102 44-45 0.28965537011394105 1102 46-47 0.35663712651464863 1102 48-49 0.47459588267078345 1102 50-51 0.42786873384420687 1102 52-53 0.39824131433875465 1102 54-55 0.3137319926288171 1102 56-57 0.2705773068409343 1102 58-59 0.13730993571125083 1102 60-61 0.19331833698931433 1102 62-63 0.34427696209231584 1102 64-65 0.36680820143065773 1102 66-67 0.2625301474290396 1102 68-69 0.19189379829741426 1102 70-71 0.21338951687520336 1102 72-73 0.2559571569566117 1102 74-75 0.23420176801169745 1102 76-77 0.023928433501314572 1102 78-79 0.28813751825320466 1102 80-81 0.32120646765976346 1102 82-83 0.2865333029281807 1102 84-85 0.4369838492934264 1102 86-87 0.4316493662758596 1102 88-89 0.42571919302937644 1102 90-91 0.5507004402451514 1102 92-93 0.6544957405639984 1102 94-95 0.4915782781918985 1102 96-97 0.6345883926904605 1102 98-99 0.9257470348089036 1102 100-101 0.990606223111488 1103 1 0.044543389210382145 1103 2 0.02903893798148971 1103 3 0.10366760010847287 1103 4 0.11002194992358483 1103 5 0.1099999320874332 1103 6 0.12426510429931881 1103 7 0.16478533439770615 1103 8 0.11125654775003113 1103 9 0.0630858710477753 1103 10-11 0.07986400960998452 1103 12-13 0.10066171922620981 1103 14-15 0.14143013740029176 1103 16-17 0.14601643699427314 1103 18-19 0.1333860001130418 1103 20-21 0.18821004854618906 1103 22-23 0.18230327165847626 1103 24-25 0.17826119784660932 1103 26-27 0.23765021667787067 1103 28-29 0.21895299586820016 1103 30-31 0.24996694214872406 1103 32-33 0.31560542419529014 1103 34-35 0.32591089576472143 1103 36-37 0.34036354498210386 1103 38-39 0.37371455361128625 1103 40-41 0.4188721280761243 1103 42-43 0.4362457341451105 1103 44-45 0.43752973360385994 1103 46-47 0.46750121028142644 1103 48-49 0.43582330618718146 1103 50-51 0.32474468093025166 1103 52-53 0.2878555175578228 1103 54-55 0.3476275025877271 1103 56-57 0.5800843442875205 1103 58-59 0.5458603991861466 1103 60-61 0.4741579159711691 1103 62-63 0.48850229690003033 1103 64-65 0.45841493717487936 1103 66-67 0.3490123114950592 1103 68-69 0.3842554725407723 1103 70-71 0.43801040942274483 1103 72-73 0.4102221499259926 1103 74-75 0.5095622409062663 1103 76-77 0.34739855677608134 1103 78-79 0.4895866452275861 1103 80-81 0.5612232592394015 1103 82-83 0.6932452932081787 1103 84-85 0.556236623999574 1103 86-87 0.6576968605080218 1103 88-89 0.45823581396288304 1103 90-91 0.5797405898676828 1103 92-93 0.7119587103528104 1103 94-95 0.9460139172958044 1103 96-97 0.575148043753245 1103 98-99 0.8851492272704427 1103 100-101 1.1290391455289317 1104 1 0.0029801943725189517 1104 2 0.09156405897903142 1104 3 0.06673778225505345 1104 4 0.09472893490377032 1104 5 0.048880528529700484 1104 6 0.09449710258255806 1104 7 0.04549383715581712 1104 8 0.07440492086197281 1104 9 0.13473697581598998 1104 10-11 0.17554633360953176 1104 12-13 0.09146435808528963 1104 14-15 0.12937840911535403 1104 16-17 0.16346261422342678 1104 18-19 0.16009529298359126 1104 20-21 0.12328237452763346 1104 22-23 0.07964462007291928 1104 24-25 0.11332571796179991 1104 26-27 0.046768870872433865 1104 28-29 0.1038511981110517 1104 30-31 0.11442283754905702 1104 32-33 0.09574804134083337 1104 34-35 0.18152949214295688 1104 36-37 0.15952682756319092 1104 38-39 0.1674522114121615 1104 40-41 0.21814533577646955 1104 42-43 0.08498029738308333 1104 44-45 0.21384363950321728 1104 46-47 0.201661321563968 1104 48-49 0.15041067089762805 1104 50-51 0.10131293899694782 1104 52-53 0.0162767295098476 1104 54-55 0.25917162465136556 1104 56-57 0.20696426810226853 1104 58-59 0.2901117755985112 1104 60-61 0.35728180512363394 1104 62-63 0.3812714583832957 1104 64-65 0.2507885529235736 1104 66-67 0.22276664738916452 1104 68-69 0.2983861808450783 1104 70-71 0.3877668267047696 1104 72-73 0.2681002799791372 1104 74-75 0.2495823747490924 1104 76-77 0.24625993226497656 1104 78-79 0.43127649355298914 1104 80-81 0.407841228766884 1104 82-83 0.2893558814291559 1104 84-85 0.3687254940250213 1104 86-87 0.46794207319670633 1104 88-89 0.5774866280882698 1104 90-91 0.5979584232820301 1104 92-93 0.3842335832164139 1104 94-95 0.5001931780882067 1104 96-97 0.48745151784001806 1104 98-99 0.6171104657200708 1104 100-101 0.8222339384075461 1105 1 0.02309777718812711 1105 2 -0.032574830007540356 1105 3 0.026309458490878512 1105 4 0.04774102122762258 1105 5 0.007334952001656347 1105 6 0.040058409064982925 1105 7 -0.07647057854459405 1105 8 0.021602168722793635 1105 9 0.026691500011494895 1105 10-11 0.07945079041319048 1105 12-13 0.07752331426778625 1105 14-15 0.008057644540706121 1105 16-17 0.02925086769712948 1105 18-19 0.003024951774392548 1105 20-21 0.032107340103280535 1105 22-23 -0.017470089735247996 1105 24-25 -0.11156925073352397 1105 26-27 -0.17599922332242102 1105 28-29 -0.0041514513453719815 1105 30-31 0.010750170504955747 1105 32-33 -0.014342172800951403 1105 34-35 0.01992646551325805 1105 36-37 0.038667231170755656 1105 38-39 0.11250449974576071 1105 40-41 0.09598562690521106 1105 42-43 0.12019850076628558 1105 44-45 0.17908685548314196 1105 46-47 0.029507691271149383 1105 48-49 0.1411319218590208 1105 50-51 0.04789517035769819 1105 52-53 0.05226743983444493 1105 54-55 0.21705184852390147 1105 56-57 0.1482332693114472 1105 58-59 0.18528028534709762 1105 60-61 0.1294492754855412 1105 62-63 0.20322261173075873 1105 64-65 0.14834164880385714 1105 66-67 0.12008049343634752 1105 68-69 0.062435358051715184 1105 70-71 0.17638526629852436 1105 72-73 0.23310110636438708 1105 74-75 0.16077409261061248 1105 76-77 -0.10041181997819493 1105 78-79 -0.027414667942785798 1105 80-81 -0.007098485298794088 1105 82-83 0.1355595580146165 1105 84-85 -0.003515723057091691 1105 86-87 0.14340752401372114 1105 88-89 0.3464647286279572 1105 90-91 0.12126286411058729 1105 92-93 0.3996169079174976 1105 94-95 0.3664142162993578 1105 96-97 0.3980231955829261 1105 98-99 0.6411232367631321 1105 100-101 0.7495104037091629 1106 1 0.02725250865396589 1106 2 0.06783101921212875 1106 3 0.06714682718351384 1106 4 0.015794178189999286 1106 5 0.0818432491324188 1106 6 0.07311538899934078 1106 7 0.12190660491405225 1106 8 0.08568156273457816 1106 9 0.07765648004063763 1106 10-11 0.10766050303499242 1106 12-13 0.02485576967974623 1106 14-15 -0.007232550708941687 1106 16-17 -0.0925379930185315 1106 18-19 0.015447555331952856 1106 20-21 0.09663504468064588 1106 22-23 -0.004350114186792098 1106 24-25 0.0902176561798882 1106 26-27 0.013076120418617165 1106 28-29 0.04398341710908937 1106 30-31 0.09232721777777186 1106 32-33 0.1945017566048861 1106 34-35 0.05288672256399707 1106 36-37 0.07042002392311275 1106 38-39 0.13624597785520365 1106 40-41 0.2029592017297901 1106 42-43 0.12220574240858895 1106 44-45 0.14395686234563243 1106 46-47 0.17055468263330908 1106 48-49 0.13813423788847246 1106 50-51 0.25654722574315514 1106 52-53 0.21834728070595588 1106 54-55 0.2962941262897729 1106 56-57 0.22509495510426092 1106 58-59 0.16237763644584646 1106 60-61 0.24637370057681096 1106 62-63 0.33072017794119546 1106 64-65 0.3122295073253625 1106 66-67 0.2753010143567849 1106 68-69 0.1663816275226324 1106 70-71 0.26019504043608066 1106 72-73 0.2500313590093981 1106 74-75 0.30652048617699634 1106 76-77 -0.0019887898727155573 1106 78-79 0.3294592823116673 1106 80-81 0.33939044984512634 1106 82-83 0.27265759584583904 1106 84-85 0.3538505466749804 1106 86-87 0.58325665082371 1106 88-89 0.8137026325850378 1106 90-91 0.886194821980034 1106 92-93 0.963690498556268 1106 94-95 1.0292151514161958 1106 96-97 1.0359555891889656 1106 98-99 1.0682828048567679 1106 100-101 1.506575405839481 1107 1 0.0209893896975899 1107 2 0.019469949912163997 1107 3 -0.008328607193519133 1107 4 0.06069900226329139 1107 5 0.03396411143965139 1107 6 0.029562270012498004 1107 7 0.07962211551900822 1107 8 0.09437226716087821 1107 9 0.016695939846044894 1107 10-11 0.09027499313542364 1107 12-13 0.07137048904041876 1107 14-15 0.1223455945070242 1107 16-17 0.04108554211678239 1107 18-19 -0.03837757321243629 1107 20-21 0.06936694892799267 1107 22-23 0.0678337612932367 1107 24-25 0.07213133593685939 1107 26-27 0.09324221516498454 1107 28-29 0.038325378342243255 1107 30-31 0.12430694782577234 1107 32-33 0.2082792825393156 1107 34-35 0.2085611772360565 1107 36-37 0.2490466910865976 1107 38-39 0.16185186067820467 1107 40-41 0.20352602500958739 1107 42-43 0.07996466552520332 1107 44-45 0.17716883984002152 1107 46-47 0.18318303661600766 1107 48-49 0.24337512987322185 1107 50-51 0.16951222881424854 1107 52-53 0.18168315903187704 1107 54-55 0.24672547441172554 1107 56-57 0.30082108062488544 1107 58-59 0.18016551764109323 1107 60-61 0.2702551517287759 1107 62-63 0.3191328454426028 1107 64-65 0.30045557718892013 1107 66-67 0.38350731483042466 1107 68-69 0.34386000153015317 1107 70-71 0.5019677543191605 1107 72-73 0.5064908595299897 1107 74-75 0.4276620747070048 1107 76-77 0.20811177248628 1107 78-79 0.48951840066155583 1107 80-81 0.5207192968674015 1107 82-83 0.28632456927082117 1107 84-85 0.4557297518592698 1107 86-87 0.4387951030604569 1107 88-89 0.6278263103196089 1107 90-91 0.7845657806618682 1107 92-93 0.9574240401759226 1107 94-95 1.0614217279488791 1107 96-97 0.9601445134093503 1107 98-99 0.6212547398689985 1107 100-101 0.7488048591839807 1108 1 0.02923819550489526 1108 2 0.07662361439034981 1108 3 0.10719895085692599 1108 4 0.09595567231141189 1108 5 0.06852949290228594 1108 6 0.11204271058042536 1108 7 0.07778498753742014 1108 8 0.06798284911327102 1108 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Count 2 4.0 3 6.0 4 24.0 5 61.0 6 139.0 7 331.0 8 813.0 9 1481.0 10 2594.0 11 3973.0 12 6015.0 13 8318.0 14 10155.0 15 13548.0 16 18102.0 17 25590.0 18 24874.0 19 17392.0 20 16417.0 21 17368.0 22 19317.0 23 21044.0 24 24347.0 25 29117.0 26 34852.0 27 42270.0 28 51568.0 29 62234.0 30 75813.0 31 92992.0 32 115020.0 33 148052.0 34 200231.0 35 288882.0 36 461313.0 37 691733.0 38 452325.0 39 25330.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.279435476323535 19.41145091034523 14.649519090644128 22.6595945226871 2 15.388625893788452 21.080439878587597 39.68012885668655 23.850805370937397 3 19.940958441521257 24.929494045072538 30.14981454212163 24.979732971284573 4 13.094048668169062 16.872189042187713 37.210264721520204 32.82349756812302 5 13.483308790946472 37.966179114922625 34.185475190659886 14.365036903471012 6 31.460188231173635 36.21966895577277 17.332362957431418 14.987779855622183 7 28.00342383775457 33.32152546554239 20.740885996257216 17.934164700445827 8 25.81501754367274 34.942255198430445 19.706510119198427 19.53621713869839 9 26.147480046756495 17.34937560905126 20.78779563643797 35.71534870775427 10-11 24.977136128180174 26.468190170149818 27.140223201994107 21.414450499675894 12-13 26.489580832900806 23.99023586992746 27.479194459269014 22.040988837902724 14-15 23.10119639168136 25.33006061029792 26.56234237743519 25.006400620585527 16-17 21.904711012916803 28.547243054386804 27.18333209314188 22.36471383955452 18-19 21.7318240739184 27.756071681776405 29.073241718829824 21.43886252547537 20-21 23.030469292829682 26.419399483361396 28.617776989106908 21.932354234702014 22-23 22.4565336738971 26.407330821498 28.538406810121163 22.59772869448374 24-25 22.600790040101277 26.46466210221248 28.898072175639932 22.036475682046312 26-27 22.577074232936532 26.286332296427027 29.21184300482763 21.92475046580881 28-29 21.93654913510143 26.976988307880383 29.132917416727068 21.95354514029112 30-31 22.269361213218463 26.779678171236498 28.759704452620433 22.191256162924606 32-33 22.12859899981589 26.740958574692698 28.77192293235523 22.35851949313618 34-35 22.73168915787174 26.5062858611743 28.637062273911447 22.12496270704251 36-37 22.7151519430181 26.286078027092643 29.00175279482277 21.997017235066487 38-39 22.53803773548468 26.566672232765253 29.029779071391626 21.865510960358446 40-41 22.410043989148544 26.643300344801407 28.871724286724547 22.0749313793255 42-43 22.047651493951463 26.9173291311171 28.346697488542095 22.688321886389335 44-45 22.427150962439466 26.813449831643215 28.17620172383628 22.583197482081037 46-47 22.708772249907444 26.96846592498089 27.786232424470835 22.536529400640827 48-49 22.720840180100424 26.866322861989982 27.8676391392642 22.54519781864539 50-51 22.518743160357023 27.21376375044987 28.02693009023418 22.240562998958925 52-53 22.662932761406385 27.396511640682142 27.5725642860163 22.36799131189517 54-55 22.136422821989402 26.9544157485883 27.88237099765718 23.026790431765118 56-57 22.601390909118148 27.3183233582375 27.37225779194426 22.70802794070009 58-59 22.878653546067532 26.899381180660818 27.521962643114666 22.70000263015698 60-61 22.569236823496038 26.829269104715536 28.179979784285813 22.421514287502614 62-63 22.603656204024027 26.7982795352388 28.005944186439834 22.592120074297338 64-65 22.361544707841162 27.13827600867352 27.862151807999876 22.63802747548544 66-67 22.258597429993355 27.05029211885049 27.585305096731496 23.105805354424668 68-69 22.114585244258237 27.16352212355792 27.762793721989514 22.959098910194324 70-71 22.52939509869169 26.931293857101053 27.38797202201191 23.15133902219535 72-73 22.336712669631282 26.69889304568746 27.647772857462943 23.316621427218312 74-75 22.060897968369787 27.420748842989873 27.319788244091264 23.198564944549076 76-77 22.349799933186098 26.76121694607152 27.435704116052918 23.45327900468947 78-79 22.192198321110506 26.861466146701236 27.69185489222193 23.25448063996633 80-81 22.275797939900276 27.05020340313639 27.45389678726585 23.220101869697476 82-83 22.17176319321145 26.988341173247054 27.50654454401599 23.3333510895255 84-85 22.104439245568962 26.81820164260642 27.36776798558816 23.709591126236457 86-87 22.038502450683087 26.824299285922805 27.567752023129867 23.56944624026425 88-89 22.17498126469104 26.525836668235048 27.403127333553375 23.896054733520536 90-91 21.99961996230708 26.470878734384236 27.639970940653452 23.889530362655233 92-93 22.083720224114217 26.799037969508397 27.442322616472577 23.674919189904806 94-95 21.892721835957026 26.677512462871906 27.42066535347413 24.009100347696936 96-97 21.463157954313537 26.456837304783154 27.628795927084642 24.451208813818667 98-99 21.658320701467247 26.57071553348313 27.143236205852418 24.6277275591972 100-101 21.921799047720036 25.756404701172762 27.960035736716883 24.361760514390323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 522.0 1 368.5 2 253.5 3 324.0 4 309.0 5 322.5 6 555.5 7 990.0 8 1623.0 9 2157.5 10 2222.5 11 2194.5 12 2415.0 13 2694.0 14 2986.5 15 3168.5 16 3451.5 17 3869.5 18 4134.0 19 4476.5 20 5199.5 21 6029.5 22 7114.0 23 8894.0 24 10737.5 25 13342.0 26 17270.0 27 21576.5 28 25444.5 29 29543.5 30 33672.0 31 38361.5 32 43868.5 33 49030.0 34 55157.0 35 60827.0 36 67036.0 37 72286.0 38 75562.0 39 78364.0 40 80527.0 41 84060.0 42 87618.0 43 91773.5 44 96807.0 45 101029.0 46 106461.5 47 120651.0 48 156107.0 49 156376.5 50 124925.5 51 115425.5 52 109952.5 53 104695.0 54 100653.0 55 94694.5 56 86047.5 57 77350.0 58 70138.5 59 62285.5 60 52480.5 61 42576.0 62 33680.0 63 25870.5 64 18699.5 65 13286.0 66 9449.5 67 6998.0 68 5044.5 69 3341.0 70 2255.0 71 1446.0 72 974.0 73 718.5 74 479.0 75 298.5 76 180.0 77 82.5 78 41.5 79 24.5 80 12.5 81 9.0 82 8.0 83 6.0 84 4.5 85 3.0 86 2.5 87 2.0 88 1.5 89 1.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3933551445958862 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001065371530010018 16-17 0.002613489534555825 18-19 3.495750332845371E-4 20-21 0.0 22-23 0.0 24-25 6.658572062562612E-5 26-27 1.664643015640653E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01328385126481241 36-37 0.02425384873788431 38-39 0.011535976098389723 40-41 0.005959421995993537 42-43 0.005077161197703991 44-45 0.0018477537473611247 46-47 0.0012984215521997093 48-49 0.003345932461437712 50-51 5.992714856306351E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.990286475075134E-4 60-61 0.0014315929934509614 62-63 0.0013317144125125223 64-65 7.990286475075134E-4 66-67 0.0024969645234609794 68-69 0.002796600266276297 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09237104093789983 78-79 0.0037953860756606886 80-81 1.9975716187687836E-4 82-83 0.0 84-85 1.9975716187687836E-4 86-87 0.09491794475183003 88-89 0.4429282136016649 90-91 0.3941375268132373 92-93 0.0 94-95 8.489679379767329E-4 96-97 0.0 98-99 0.0 100-101 4.494536142229763E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3003647.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.83152296280065 #Duplication Level Percentage of deduplicated Percentage of total 1 71.0307415358772 35.39570027909822 2 15.204103600400154 15.152872753842805 3 5.66160521590062 8.46379230967391 4 2.696194811789551 5.374219747034999 5 1.488611298507208 3.708988405212321 6 0.9268980423872968 2.7713244650038527 7 0.6181030103084568 2.1560710046093448 8 0.4359283133974806 1.7378377407361207 9 0.313794093092074 1.4073153799958005 >10 1.496152043899428 13.033540214777567 >50 0.08602709689901272 2.911516126604199 >100 0.03757004231066388 3.263027534918 >500 0.0017350511877506105 0.5801482312010169 >1k 0.0021354476152365466 2.385438106730207 >5k 3.336636898807104E-4 1.2665095454446522 >10k+ 6.673273797614208E-5 0.39169815511717054 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11779 0.39215660162462496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9378 0.31222044401356086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8146 0.27120364010817516 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7982 0.2657436110168738 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6820 0.22705730733338503 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5760 0.1917668754018032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4856 0.16167012967902022 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4470 0.14881908559827436 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4145 0.1379989059966101 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4028 0.13410364134001101 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3442 0.11459402519670255 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.658572062562611E-5 2 0.0 0.0 0.0 0.0 6.658572062562611E-5 3 0.0 0.0 0.0 0.0 6.658572062562611E-5 4 0.0 0.0 0.0 0.0 6.658572062562611E-5 5 0.0 0.0 0.0 0.0 6.658572062562611E-5 6 0.0 0.0 0.0 0.0 6.658572062562611E-5 7 0.0 0.0 0.0 0.0 6.658572062562611E-5 8 0.0 0.0 0.0 0.0 6.658572062562611E-5 9 0.0 0.0 0.0 0.0 6.658572062562611E-5 10-11 0.0 0.0 0.0 0.0 6.658572062562611E-5 12-13 0.0 0.0 0.0 0.0 9.987858093843917E-5 14-15 0.0 1.6646430156406527E-5 0.0 0.0 9.987858093843917E-5 16-17 0.0 3.3292860312813054E-5 0.0 1.6646430156406527E-5 9.987858093843917E-5 18-19 1.6646430156406527E-5 3.3292860312813054E-5 0.0 6.658572062562611E-5 9.987858093843917E-5 20-21 3.3292860312813054E-5 6.658572062562611E-5 0.0 8.323215078203264E-5 1.4981787140765876E-4 22-23 3.3292860312813054E-5 6.658572062562611E-5 0.0 2.663428825025045E-4 1.664643015640653E-4 24-25 6.658572062562611E-5 6.658572062562611E-5 0.0 6.325643459434481E-4 1.664643015640653E-4 26-27 6.658572062562611E-5 6.658572062562611E-5 0.0 0.0012151894014176766 1.664643015640653E-4 28-29 1.664643015640653E-4 6.658572062562611E-5 0.0 0.00402843609785038 1.664643015640653E-4 30-31 1.9975716187687834E-4 9.987858093843917E-5 0.0 0.012434883326835677 1.664643015640653E-4 32-33 2.1640359203328486E-4 9.987858093843917E-5 0.0 0.02723355973588108 1.664643015640653E-4 34-35 2.496964523460979E-4 9.987858093843917E-5 0.0 0.052602719294244635 2.330500221896914E-4 36-37 3.662214634409436E-4 9.987858093843917E-5 0.0 0.09080627650319761 2.330500221896914E-4 38-39 3.8286789359735015E-4 1.1652501109484569E-4 0.0 0.14710450329216448 2.330500221896914E-4 40-41 3.9951432375375667E-4 1.3317144125125221E-4 0.0 0.2113763701260501 2.330500221896914E-4 42-43 4.328071840665697E-4 1.3317144125125221E-4 0.0 0.28065881243701407 2.330500221896914E-4 44-45 4.661000443793828E-4 1.3317144125125221E-4 0.0 0.36582194911718985 2.663428825025045E-4 46-47 4.993929046921958E-4 1.3317144125125221E-4 0.0 0.4691130482376924 3.1628217297172405E-4 48-49 4.993929046921958E-4 1.3317144125125221E-4 0.0 0.5820923697092235 3.329286031281306E-4 50-51 4.993929046921958E-4 1.3317144125125221E-4 0.0 0.7008646488751841 3.329286031281306E-4 52-53 5.160393348486023E-4 1.3317144125125221E-4 0.0 0.8216344996599134 3.662214634409436E-4 54-55 5.326857650050089E-4 1.3317144125125221E-4 0.0 0.9532578229066199 3.662214634409436E-4 56-57 5.326857650050089E-4 1.664643015640653E-4 0.0 1.1160932026965886 3.662214634409436E-4 58-59 5.326857650050089E-4 1.9975716187687834E-4 0.0 1.3013346774770804 3.662214634409436E-4 60-61 5.65978625317822E-4 2.330500221896914E-4 0.0 1.5154077692884682 3.662214634409436E-4 62-63 5.992714856306351E-4 2.330500221896914E-4 0.0 1.7263346858002953 3.662214634409436E-4 64-65 5.992714856306351E-4 2.496964523460979E-4 0.0 1.9463172603172076 3.9951432375375667E-4 66-67 5.992714856306351E-4 2.6634288250250443E-4 0.0 2.199226473683492 3.9951432375375667E-4 68-69 5.992714856306351E-4 2.6634288250250443E-4 0.0 2.4913713229284267 3.9951432375375667E-4 70-71 6.325643459434481E-4 2.6634288250250443E-4 0.0 2.8112990641044036 3.9951432375375667E-4 72-73 6.325643459434481E-4 2.6634288250250443E-4 0.0 3.1386011738396693 4.161607539101632E-4 74-75 6.325643459434481E-4 2.9963574281531753E-4 0.0 3.480152627788818 4.328071840665697E-4 76-77 6.325643459434481E-4 2.9963574281531753E-4 0.0 3.8431779766397316 4.4945361422297624E-4 78-79 6.325643459434481E-4 2.9963574281531753E-4 0.0 4.260387455649749 4.661000443793828E-4 80-81 7.324429268818872E-4 2.9963574281531753E-4 0.0 4.723391264020039 4.661000443793828E-4 82-83 7.657357871947003E-4 2.9963574281531753E-4 0.0 5.199345995052015 4.661000443793828E-4 84-85 7.657357871947003E-4 2.9963574281531753E-4 0.0 5.695925653047778 4.661000443793828E-4 86-87 7.990286475075133E-4 2.9963574281531753E-4 0.0 6.229460385990764 4.661000443793828E-4 88-89 7.990286475075133E-4 2.9963574281531753E-4 0.0 6.79259247175184 4.661000443793828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 720 0.0 26.364141 3 GTACATG 14005 0.0 23.401066 1 CTTTGCG 820 0.0 22.570276 2 TACATGG 14320 0.0 22.435293 2 ACATGGG 14320 0.0 21.573673 3 GAGTACT 9590 0.0 21.378647 12-13 GTACTTT 10295 0.0 19.684145 14-15 TTGCGTT 920 0.0 19.601166 4 AGTACTT 10170 0.0 19.389433 12-13 CATGGGA 9140 0.0 18.016457 4 CATGGGG 5845 0.0 17.455812 4 GTAAGCG 350 0.0 16.94866 94-95 ACTTTTT 12515 0.0 16.305517 16-17 GTATAGG 770 0.0 16.0847 1 TACTTTT 12325 0.0 16.018486 14-15 TACCTGG 2285 0.0 15.160823 2 CCGTCTA 220 5.7446514E-4 15.099714 9 AGTACAT 10730 0.0 15.037144 2 GTGTAGC 1645 0.0 14.76844 1 TAAGGTG 995 0.0 14.308178 5 >>END_MODULE