FastQCFastQC Report
Fri 27 May 2016
SRR939028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020773
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114710.3797372394416926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98000.32442027255937467No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA80120.26523012487201125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77860.2577485961374787No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA69180.2290142291393627No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61350.20309371144405755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.15615208425128269No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46790.15489412809237899No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT44110.14602222676116344No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40980.13566064050493035No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35110.11622852826081272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139800.023.707171
TACATGG142100.022.9823272
GAGTACT93150.021.6711712-13
ACATGGG142100.021.6500173
GTATAGC9550.019.8594111
GTACTTT100300.019.65425714-15
AGTACTT97950.019.28033612-13
CATGGGG56700.018.6149024
TGCACCG4600.018.5205385
CATGGGA87600.018.4782564
ACTTTTT123500.016.4618416-17
TTTGCGT7500.016.4078273
GCACCGT6400.016.268976
GTGTAGC17500.016.2563461
AGTACAT107550.016.2388362
GTATAGA8500.016.1766571
TACTTTT119050.015.52509214-15
TATACGT3102.143779E-615.2678285
ATGGGAG37750.014.9200055
CTTTGCG8600.014.8595032
Produced by FastQC (version 0.11.5)