##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3020773 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.896527478231565 33.0 31.0 34.0 30.0 34.0 2 31.956849455420848 33.0 31.0 34.0 30.0 34.0 3 32.01375839892637 34.0 31.0 34.0 30.0 34.0 4 35.536173687993106 37.0 35.0 37.0 33.0 37.0 5 35.57638591181794 37.0 35.0 37.0 33.0 37.0 6 35.657636638039335 37.0 35.0 37.0 33.0 37.0 7 35.577338979128854 37.0 35.0 37.0 33.0 37.0 8 35.56151984938954 37.0 35.0 37.0 33.0 37.0 9 37.18232485526056 39.0 37.0 39.0 34.0 39.0 10-11 37.07038297813176 39.0 37.0 39.0 33.0 39.0 12-13 36.960357332378166 39.0 37.0 39.0 33.0 39.0 14-15 38.187476516772364 40.0 38.0 41.0 33.0 41.0 16-17 38.125071629016816 40.0 38.0 41.0 33.0 41.0 18-19 38.07872686891733 40.0 38.0 41.0 33.0 41.0 20-21 37.95802829275818 40.0 38.0 41.0 33.0 41.0 22-23 38.04318431077078 40.0 38.0 41.0 33.0 41.0 24-25 37.98767649869752 40.0 38.0 41.0 33.0 41.0 26-27 37.90470220701788 40.0 38.0 41.0 33.0 41.0 28-29 37.78561447682431 40.0 38.0 41.0 32.0 41.0 30-31 37.61641540095863 40.0 37.0 41.0 32.0 41.0 32-33 37.4349146725027 40.0 37.0 41.0 31.5 41.0 34-35 37.12513767171515 40.0 36.5 41.0 31.0 41.0 36-37 36.86634646165071 40.0 36.0 41.0 30.0 41.0 38-39 36.66217885289626 39.5 36.0 41.0 30.0 41.0 40-41 36.51658400018803 39.0 36.0 41.0 30.0 41.0 42-43 36.32159963691413 39.0 35.5 41.0 30.0 41.0 44-45 36.25339424710165 39.0 36.0 41.0 30.0 41.0 46-47 36.026432803788964 39.0 35.0 41.0 28.5 41.0 48-49 35.841762191333146 39.0 35.0 41.0 27.5 41.0 50-51 34.6743883105417 38.0 34.0 40.0 25.5 40.5 52-53 34.80987714071862 38.5 34.5 39.5 26.0 40.5 54-55 35.31136003930119 39.0 35.0 40.5 26.0 41.0 56-57 35.207135061125086 39.0 35.0 41.0 26.0 41.0 58-59 35.01261597610943 38.5 35.0 40.5 26.0 41.0 60-61 34.73403860535035 38.0 34.0 40.0 26.0 41.0 62-63 34.37300502222445 38.0 34.0 40.0 24.5 41.0 64-65 33.96255163827272 37.0 34.0 40.0 23.0 41.0 66-67 33.57637217361251 36.5 33.5 39.5 22.5 41.0 68-69 33.18161824804446 36.0 33.0 39.0 22.0 41.0 70-71 32.76172373759961 35.5 32.5 39.0 21.5 40.0 72-73 32.32307790092139 35.0 32.0 38.0 20.0 40.0 74-75 31.862850336652244 35.0 32.0 37.0 18.5 39.5 76-77 31.449970255957666 35.0 31.0 37.0 18.0 39.0 78-79 31.028610392108245 35.0 31.0 36.0 14.5 39.0 80-81 30.599080765088935 34.5 31.0 36.0 9.5 37.5 82-83 30.231853734126993 34.0 30.5 35.0 7.0 37.0 84-85 29.88918084874302 34.0 30.0 35.0 3.5 37.0 86-87 29.511458656443235 34.0 30.0 35.0 2.0 36.0 88-89 29.10176137035123 34.0 29.0 35.0 2.0 36.0 90-91 28.68313689906524 34.0 29.0 35.0 2.0 35.5 92-93 28.282133248675088 34.0 29.0 35.0 2.0 35.0 94-95 27.830714853449763 34.0 28.0 35.0 2.0 35.0 96-97 27.26741085808169 34.0 27.0 35.0 2.0 35.0 98-99 26.579356012517323 33.5 25.5 35.0 2.0 35.0 100-101 23.95739683187052 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26559579988253645 1101 2 0.2661579880135392 1101 3 0.2760960006647011 1101 4 0.19310451369166515 1101 5 0.11161482013858404 1101 6 0.20738913603543097 1101 7 0.1972252282541902 1101 8 0.2763877229009424 1101 9 0.29763357811857105 1101 10-11 0.31287561225741456 1101 12-13 0.4762833713231558 1101 14-15 0.2342816232569973 1101 16-17 0.45776505193752826 1101 18-19 0.47035729926701464 1101 20-21 0.5455407016496778 1101 22-23 0.4698370389253128 1101 24-25 0.5654003250036581 1101 26-27 0.5564565886064727 1101 28-29 0.4566593228385045 1101 30-31 0.3854235309362579 1101 32-33 0.4686436300969419 1101 34-35 0.4395938863661044 1101 36-37 0.3779800307232577 1101 38-39 0.4140687331676318 1101 40-41 0.40110450392720054 1101 42-43 0.387825905278838 1101 44-45 0.48692395337516814 1101 46-47 0.5848579428491476 1101 48-49 0.5439095413570172 1101 50-51 0.5883246134210083 1101 52-53 0.6303201897841291 1101 54-55 0.5640337351956859 1101 56-57 0.6152520050963801 1101 58-59 0.484954008524376 1101 60-61 0.3958730150412677 1101 62-63 0.4465318954853785 1101 64-65 0.33776266029295954 1101 66-67 0.36709306733049374 1101 68-69 0.3398476114498621 1101 70-71 0.309435527377417 1101 72-73 0.2791528709893569 1101 74-75 0.20875488932864172 1101 76-77 0.13661176226190364 1101 78-79 0.07449463249706056 1101 80-81 -0.01855049270742981 1101 82-83 -0.022750689260718104 1101 84-85 -0.13738433026864527 1101 86-87 -0.1680682223077561 1101 88-89 -0.15953841323538498 1101 90-91 -0.2560132339640617 1101 92-93 -0.13790067718029775 1101 94-95 -0.15832504924533097 1101 96-97 -0.373712448116855 1101 98-99 -0.4951448652912198 1101 100-101 -0.2528303846649784 1102 1 0.13484046795523952 1102 2 0.04815567238996721 1102 3 0.04070521368421964 1102 4 0.11332962392606305 1102 5 0.1175853290912201 1102 6 0.11973207274596831 1102 7 0.22780790851196997 1102 8 0.1037443416824857 1102 9 0.15813880739845843 1102 10-11 0.13423220298624727 1102 12-13 0.16643693439967677 1102 14-15 0.2204295145859092 1102 16-17 0.22215551758699092 1102 18-19 0.19772086746952766 1102 20-21 0.3033449013951568 1102 22-23 0.2987493079048775 1102 24-25 0.27473747775575674 1102 26-27 0.31052767416978355 1102 28-29 0.14818147555234873 1102 30-31 0.106894117671402 1102 32-33 0.07716017736958491 1102 34-35 0.14300624332916811 1102 36-37 0.12087625610381991 1102 38-39 0.06716996009109266 1102 40-41 0.10749427335943551 1102 42-43 -0.1511540344843425 1102 44-45 0.11831659183441445 1102 46-47 0.13732071562986192 1102 48-49 0.20919845875199172 1102 50-51 0.17085963467731347 1102 52-53 0.02733047237691011 1102 54-55 0.09667548425655781 1102 56-57 0.23100455725528946 1102 58-59 0.09972433334923636 1102 60-61 0.20321938183298727 1102 62-63 -0.0058131196758282044 1102 64-65 0.009107009839915747 1102 66-67 -0.03251993280466792 1102 68-69 -0.06947755514497089 1102 70-71 -0.07435313116756959 1102 72-73 -0.18165921666771112 1102 74-75 -0.19828595796516524 1102 76-77 -0.2981504527799572 1102 78-79 -0.3817713862370482 1102 80-81 -0.38593928284511847 1102 82-83 -0.45478879503983194 1102 84-85 -0.4286184326712359 1102 86-87 -0.355429457034969 1102 88-89 -0.2475801811680789 1102 90-91 -0.2414862026291864 1102 92-93 -0.36761723006348035 1102 94-95 -0.5635149278281055 1102 96-97 -0.8254268511424669 1102 98-99 -0.6654822501157263 1102 100-101 -0.3214829493200959 1103 1 -0.022440133409492802 1103 2 0.10382788724353986 1103 3 0.2016956264122598 1103 4 0.3002336434282995 1103 5 0.23350416279451025 1103 6 0.20791005497689952 1103 7 0.20183574019613104 1103 8 0.12330140460935723 1103 9 0.2921772050799234 1103 10-11 0.23945670239409367 1103 12-13 0.35124164723318785 1103 14-15 0.34368629635508086 1103 16-17 0.42782770129080916 1103 18-19 0.3916185679322268 1103 20-21 0.41266117047671713 1103 22-23 0.43718667415561896 1103 24-25 0.48428332054044887 1103 26-27 0.47265312171391116 1103 28-29 0.39873964803353346 1103 30-31 0.4497636581315163 1103 32-33 0.43337539477307274 1103 34-35 0.44131026370359905 1103 36-37 0.46957989670043077 1103 38-39 0.4622954593438138 1103 40-41 0.45865656421218404 1103 42-43 0.3323622621336071 1103 44-45 0.503978359059758 1103 46-47 0.48098582086308284 1103 48-49 0.37603867109364586 1103 50-51 0.1707202083685999 1103 52-53 0.3046722020231982 1103 54-55 0.38473204473785927 1103 56-57 0.37949793699760903 1103 58-59 0.41808325205398233 1103 60-61 0.34014037795234486 1103 62-63 0.29859376093151013 1103 64-65 0.2225760398178167 1103 66-67 0.22625087289623025 1103 68-69 0.2596049163265235 1103 70-71 0.2135127928058438 1103 72-73 0.12276118293382865 1103 74-75 0.10251334477868923 1103 76-77 -0.011600107609510246 1103 78-79 0.2381431512918688 1103 80-81 0.12423534891803811 1103 82-83 0.2683576542929096 1103 84-85 0.18471142019600606 1103 86-87 0.12281443185011298 1103 88-89 0.1297429209240022 1103 90-91 -0.0342013337478555 1103 92-93 0.08416219975892147 1103 94-95 -0.05193936693002499 1103 96-97 -0.29750782306470214 1103 98-99 -0.31745113275527714 1103 100-101 -0.18608922574676967 1104 1 0.0668956804801546 1104 2 0.10736084163023207 1104 3 0.074567850799653 1104 4 0.10118223561454442 1104 5 0.16022664197818415 1104 6 0.11294335374912379 1104 7 0.09567650907054315 1104 8 0.09222459315369491 1104 9 0.19720413088138145 1104 10-11 0.10248961143463475 1104 12-13 0.12431498787092465 1104 14-15 0.18112774468621495 1104 16-17 0.02590894686343148 1104 18-19 0.15260422139458996 1104 20-21 0.2131502959825795 1104 22-23 0.1929577055719136 1104 24-25 0.23677435548638215 1104 26-27 0.2278060362450205 1104 28-29 0.19704517315406633 1104 30-31 0.2952411161985964 1104 32-33 0.3398551127936287 1104 34-35 0.22848570178406646 1104 36-37 0.20948921667993403 1104 38-39 0.2533726874937088 1104 40-41 0.42628009567137326 1104 42-43 0.3481178390957993 1104 44-45 0.28300399310906954 1104 46-47 0.3948706857928741 1104 48-49 0.4668408197652525 1104 50-51 0.51625649385808 1104 52-53 0.44328161980328673 1104 54-55 0.3086696582563988 1104 56-57 0.32040762955079316 1104 58-59 0.31332938353817497 1104 60-61 0.27454363503063206 1104 62-63 0.27852797226412207 1104 64-65 0.12387937361686596 1104 66-67 0.15018634166499822 1104 68-69 0.23356845067805665 1104 70-71 0.29321561627700277 1104 72-73 0.17846505304287774 1104 74-75 0.19388452620491492 1104 76-77 0.16215985934908872 1104 78-79 0.23154614609789448 1104 80-81 0.07035286159657161 1104 82-83 0.13674606480046592 1104 84-85 0.07010892199146923 1104 86-87 0.08857742351867515 1104 88-89 0.07123604669457961 1104 90-91 0.17097596965212603 1104 92-93 0.07453447929703216 1104 94-95 -0.027712886256058056 1104 96-97 0.08490835398623275 1104 98-99 0.03544146062712272 1104 100-101 0.26553575923998096 1105 1 0.019576680329183915 1105 2 0.07896127778755613 1105 3 0.11623729787464043 1105 4 0.043646950458239075 1105 5 0.09608640812099623 1105 6 0.12297210908691625 1105 7 0.12382867573034417 1105 8 0.1343209216422565 1105 9 0.12676148041160218 1105 10-11 0.12096767271152942 1105 12-13 0.2541242069003715 1105 14-15 0.32464366684235557 1105 16-17 0.21039649276383443 1105 18-19 0.2013616983266644 1105 20-21 0.2073659818395015 1105 22-23 0.27855423212019303 1105 24-25 0.25521053316559517 1105 26-27 0.1494049106410813 1105 28-29 0.24214126643191491 1105 30-31 0.3173828091194082 1105 32-33 0.1835037609945971 1105 34-35 0.19748314333514827 1105 36-37 0.15419819429894943 1105 38-39 0.18154669514823496 1105 40-41 0.1400011504376124 1105 42-43 0.13771440303170834 1105 44-45 0.14447559106061192 1105 46-47 0.31460029759107755 1105 48-49 0.26627926010525726 1105 50-51 0.1539500635190194 1105 52-53 0.1508273876905477 1105 54-55 0.2679886167604053 1105 56-57 0.2717016197514539 1105 58-59 0.21873125547469385 1105 60-61 0.32956353802159555 1105 62-63 0.19564506507865076 1105 64-65 0.2785087061437608 1105 66-67 0.2100794722343906 1105 68-69 0.21377933814180494 1105 70-71 -0.009028256058002171 1105 72-73 0.24208499559846075 1105 74-75 0.1836918450914773 1105 76-77 0.11735263274841401 1105 78-79 0.12372522427067523 1105 80-81 0.15061342963280921 1105 82-83 0.03413083639761538 1105 84-85 0.19645724255385488 1105 86-87 0.2717491263921765 1105 88-89 0.2926445163625715 1105 90-91 0.33305863036327565 1105 92-93 0.3951128373558852 1105 94-95 0.4525252728722684 1105 96-97 0.22538943088214936 1105 98-99 0.2519014542191691 1105 100-101 0.524597642373859 1106 1 0.13201266978682114 1106 2 0.13789972417238516 1106 3 0.13696112019777473 1106 4 0.14723294844811363 1106 5 0.1413681952691519 1106 6 0.04946066752156497 1106 7 0.12224718280887714 1106 8 0.03308783713801233 1106 9 0.15225516476628798 1106 10-11 0.1629022145729806 1106 12-13 0.13799322139617942 1106 14-15 0.2535229252171334 1106 16-17 0.2730520878647269 1106 18-19 0.21733827280169038 1106 20-21 0.2535400485169603 1106 22-23 0.10775016982827168 1106 24-25 0.17759011754228737 1106 26-27 0.1948304593840433 1106 28-29 0.20961976305484598 1106 30-31 0.17279660425590748 1106 32-33 0.23022426018654585 1106 34-35 0.07580960607187848 1106 36-37 0.04022657486842718 1106 38-39 0.14763926538517325 1106 40-41 0.25583959378798227 1106 42-43 0.2752381176374783 1106 44-45 0.3903460507873433 1106 46-47 0.45137584039782297 1106 48-49 0.4202838108635447 1106 50-51 0.2859622054894828 1106 52-53 0.22834580873133348 1106 54-55 0.14278380552364922 1106 56-57 0.23111279487326897 1106 58-59 0.2621369884240323 1106 60-61 0.3382758829543562 1106 62-63 0.3046893885320898 1106 64-65 0.07085367812813104 1106 66-67 0.2420515432563164 1106 68-69 0.3143028449509231 1106 70-71 0.20935743276368157 1106 72-73 0.1955526787188262 1106 74-75 0.13931012552051314 1106 76-77 0.14903712289145687 1106 78-79 0.19448985019573328 1106 80-81 0.15103254198688276 1106 82-83 0.3528474653335927 1106 84-85 0.2953248373173807 1106 86-87 0.34484912827284475 1106 88-89 0.44382448638031846 1106 90-91 0.45066859500323275 1106 92-93 0.4639238747388603 1106 94-95 0.4912835603647707 1106 96-97 0.5673470945923995 1106 98-99 0.4425567313369534 1106 100-101 0.6915995796940351 1107 1 -0.015199644733428386 1107 2 0.04928597954744163 1107 3 0.016814842595202606 1107 4 -0.0026862221407171205 1107 5 0.06679302575223289 1107 6 0.04415696873283537 1107 7 -0.013931676181776709 1107 8 0.09649762688059127 1107 9 0.03850193606379548 1107 10-11 0.09034456826669413 1107 12-13 0.03815484580349704 1107 14-15 0.007691559275791349 1107 16-17 0.05117518213296535 1107 18-19 0.055737497133016234 1107 20-21 0.10018734870450174 1107 22-23 0.027327888351031504 1107 24-25 0.14965429834320076 1107 26-27 0.1579439816879642 1107 28-29 0.15222772641523363 1107 30-31 0.07215544095480197 1107 32-33 0.1656008821090964 1107 34-35 0.01070355733378392 1107 36-37 0.04104811876295145 1107 38-39 -0.025590632457273443 1107 40-41 0.006281253216023686 1107 42-43 0.02287673101501042 1107 44-45 0.12444155063339224 1107 46-47 0.13093144841716509 1107 48-49 0.09265321848009478 1107 50-51 0.15804300874523847 1107 52-53 -0.04686089935265869 1107 54-55 -0.0908808756257784 1107 56-57 -0.009966200905616063 1107 58-59 0.03799821283704574 1107 60-61 0.023637362267940887 1107 62-63 -0.0460828386382488 1107 64-65 0.06003302214113404 1107 66-67 -0.008122711463478538 1107 68-69 0.015068487380695217 1107 70-71 0.06434504106201189 1107 72-73 -0.06319074399719682 1107 74-75 0.03985203590287867 1107 76-77 -0.013193332933195023 1107 78-79 0.08959235189239578 1107 80-81 0.09548216780383711 1107 82-83 0.13661760557709712 1107 84-85 0.01943952239011537 1107 86-87 0.12595623424036617 1107 88-89 0.13528143938454207 1107 90-91 0.07678077374475123 1107 92-93 0.1070254871513967 1107 94-95 -0.09930772868882087 1107 96-97 -0.07911784040221548 1107 98-99 -0.07326115768240626 1107 100-101 0.22519391654182996 1108 1 0.14777926292137167 1108 2 0.13469002886586878 1108 3 0.14884608397679955 1108 4 0.1368639475614657 1108 5 0.1055046129364996 1108 6 0.19579405242929226 1108 7 0.2930973588875574 1108 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2316 70-71 -1.7376036269176112 2316 72-73 -1.6659641826711749 2316 74-75 -1.7788885564191865 2316 76-77 -1.7483385710051529 2316 78-79 -1.5397948803520087 2316 80-81 -1.5164751741589413 2316 82-83 -1.6925739505872635 2316 84-85 -1.9477733734746536 2316 86-87 -1.8862395291045537 2316 88-89 -1.9231155954927992 2316 90-91 -2.1456958050523482 2316 92-93 -2.3597902811397375 2316 94-95 -2.5262662637542697 2316 96-97 -2.6006566024794395 2316 98-99 -2.9734090100794965 2316 100-101 -3.4332372638838997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8813.0 3 2138.0 4 2138.0 5 2378.0 6 2834.0 7 3471.0 8 4473.0 9 5392.0 10 6857.0 11 8881.0 12 11755.0 13 17667.0 14 17096.0 15 15816.0 16 19799.0 17 26581.0 18 22816.0 19 16214.0 20 16328.0 21 17585.0 22 19228.0 23 21719.0 24 24557.0 25 28506.0 26 33250.0 27 38426.0 28 45662.0 29 54658.0 30 66193.0 31 80957.0 32 100846.0 33 130252.0 34 176872.0 35 255023.0 36 398866.0 37 629364.0 38 587214.0 39 100108.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36563862757695 18.799685927667085 14.158752608277764 22.675922836478208 2 15.15850230029207 20.597197901325174 40.44405494194186 23.800244856440898 3 19.972279453329346 24.866118971046177 29.94713679158695 25.214464784037528 4 12.75167985407904 16.347541891961754 37.66528451803532 33.23549373592389 5 13.512925049229917 37.8351230201155 34.214412452269684 14.4375394783849 6 31.3850158256 35.859587020258026 17.66672376450818 15.088673389633794 7 28.004682717089267 33.299181617982796 20.914269695610134 17.7818659693178 8 25.708137882407044 34.60529267357649 20.016924998202466 19.669644445813994 9 26.39283759263162 17.40997286826329 20.900052619929035 35.297136919176054 10-11 25.00026921093364 26.29483418180336 27.262800731392268 21.44209587587073 12-13 26.58598689602625 23.87612475332922 27.488423807037883 22.049464543606657 14-15 23.266969844734323 25.172494866534876 26.631288712242128 24.929246576488673 16-17 21.95092817322587 28.411411436286976 27.26478408893233 22.372876301554825 18-19 21.793763803950352 27.656795478683303 29.121898926502094 21.42754179086425 20-21 23.16117247799027 26.34277812186531 28.562374674539036 21.933674725605382 22-23 22.523159698222543 26.34500911672574 28.52516847672712 22.6066627083246 24-25 22.64941498893222 26.394858107718406 28.827879660548394 22.127847242800975 26-27 22.59936793283408 26.174999204446614 29.227056903281923 21.998575959437385 28-29 21.95456394050973 26.924814494779802 29.145872216758796 21.97474934795168 30-31 22.287255417783506 26.74629734734714 28.728425186737976 22.238022048131377 32-33 22.14895150666545 26.686744492406607 28.781626718145436 22.382677282782502 34-35 22.777450999940182 26.48847858885145 28.568031822622775 22.166038588585597 36-37 22.79927167961895 26.1845861940132 28.965120406623345 22.0510217197445 38-39 22.606360310617653 26.472184378291924 28.983101408859607 21.938353902230816 40-41 22.48268684692936 26.588303737005152 28.835419209945112 22.093590206120375 42-43 22.067399952960752 26.82708615156858 28.377467167819425 22.72804672765124 44-45 22.42176468913569 26.767833248952005 28.160549727851482 22.649852334060828 46-47 22.73878477400083 26.893503171056604 27.786423036829806 22.58128901811276 48-49 22.702402274849067 26.784456285863996 27.91232347000399 22.600817969282943 50-51 22.509374320505362 27.187427472011898 28.05418375566197 22.24901445182077 52-53 22.626710049126604 27.347915807125872 27.675315520151635 22.35005862359589 54-55 22.148776703425497 26.907513584785043 27.974118986734858 22.969590725054605 56-57 22.60155164786441 27.28610417351134 27.422931061284668 22.689413117339583 58-59 22.891319228722796 26.85025860523761 27.576669958071616 22.68175220796797 60-61 22.564286307173347 26.74374253439812 28.274730489796713 22.41724066863182 62-63 22.633076404739775 26.698328854394997 28.13792577657866 22.53066896428657 64-65 22.390460564604318 27.06716047466714 27.997621435587845 22.544757525140696 66-67 22.278277917523795 26.960298733758965 27.679703904243187 23.081719444474054 68-69 22.106151268330304 27.09461902459651 27.912060167185476 22.887169539887704 70-71 22.539320019544682 26.814669570613166 27.558736470186197 23.087273939655955 72-73 22.32631409794735 26.59587205347397 27.861815620386782 23.215998228191896 74-75 22.111531460693428 27.35235213982804 27.518654712597286 23.017461686881248 76-77 22.418888338354737 26.673046647113825 27.6018565876295 23.30620842690194 78-79 22.271370153667462 26.70994769557068 27.89269394193764 23.12598820882422 80-81 22.343748439564976 26.943494775247423 27.684813763120136 23.027943022067465 82-83 22.27850507677674 26.847154677802713 27.78774921518628 23.086591030234263 84-85 22.267583798492048 26.616817768330314 27.674279686582253 23.441318746595385 86-87 22.199049623819832 26.703173497339392 27.824913706209074 23.2728631726317 88-89 22.39051779079656 26.441670867766256 27.650621907030608 23.517189434406575 90-91 22.175252463608892 26.32961447628423 27.975633746225764 23.519499313881116 92-93 22.29138648479241 26.648485236038866 27.83998675776366 23.220141521405065 94-95 22.145215494222164 26.527871378725727 27.778973982627775 23.547939144424333 96-97 21.735571966693925 26.2739017106805 28.037968644545767 23.952557678079806 98-99 22.0591622169137 26.332473774003894 27.570971929295762 24.037392079786645 100-101 22.28129451510625 25.78751156268959 28.122692040540837 23.80850188166332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3007.0 1 1706.0 2 440.0 3 496.0 4 460.0 5 444.0 6 700.0 7 1372.5 8 2256.5 9 2790.5 10 2866.5 11 2882.5 12 3163.5 13 3559.5 14 3896.0 15 4323.5 16 4764.0 17 5069.5 18 5232.5 19 5524.0 20 5951.0 21 6498.5 22 7546.0 23 9017.0 24 10646.0 25 13072.5 26 17088.0 27 21578.0 28 25245.5 29 29211.0 30 33653.5 31 38479.0 32 44032.0 33 49300.0 34 55302.0 35 60842.0 36 66870.5 37 72971.5 38 76573.5 39 78803.0 40 81367.5 41 85386.0 42 88689.0 43 92407.5 44 97317.5 45 101309.0 46 106882.0 47 120989.5 48 156854.5 49 157496.0 50 123810.0 51 112209.5 52 107114.5 53 103266.5 54 99888.0 55 94189.0 56 85878.5 57 77278.5 58 69733.5 59 61644.5 60 52153.5 61 42390.0 62 33646.0 63 26050.5 64 18958.0 65 13615.0 66 9739.5 67 7244.0 68 5304.5 69 3663.0 70 2589.5 71 1707.0 72 1185.5 73 897.0 74 596.5 75 378.0 76 259.5 77 138.0 78 81.0 79 62.0 80 40.0 81 36.0 82 36.0 83 29.5 84 22.0 85 19.0 86 16.5 87 16.5 88 13.5 89 7.5 90 6.5 91 5.5 92 2.5 93 2.5 94 3.5 95 3.5 96 4.5 97 3.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2889326672345125 2 0.08865280509326587 3 0.09252598589831146 4 0.10755525158626617 5 0.10921045705850788 6 0.0848127283976651 7 0.09490948177833952 8 0.09110250919218359 9 0.09616743793724322 10-11 0.08916591878966079 12-13 0.09380049411193758 14-15 0.10177858448814261 16-17 0.09097009275440425 18-19 0.10156340777675119 20-21 0.1036158625623309 22-23 0.1164106008627593 24-25 0.12933775560096705 26-27 0.13258195832656078 28-29 0.12387557754256939 30-31 0.11621197620609029 32-33 0.11083255842130474 34-35 0.3837759407939624 36-37 0.5859096330641197 38-39 0.5696886194361509 40-41 0.5336713483601714 42-43 0.5594097934535299 44-45 0.5806957358265583 46-47 0.5693906824511474 48-49 0.5908586974261224 50-51 0.5833606166368674 52-53 0.5864724029246818 54-55 0.5764418577628971 56-57 0.549925466097585 58-59 0.5456715880339237 60-61 0.4933670951110858 62-63 0.5655837098649915 64-65 0.5998961193045621 66-67 0.6124591288388767 68-69 0.6137005329430579 70-71 0.6098439041927348 72-73 0.6021803028562557 74-75 0.5996312864290034 76-77 0.5835923454029812 78-79 0.560005667423537 80-81 0.5562483510015483 82-83 0.5691920577944785 84-85 0.5792226029562632 86-87 0.5702348372419906 88-89 0.5823343892440775 90-91 0.5963208754845201 92-93 0.6044479343532267 94-95 0.6032065302490455 96-97 0.6004257850556795 98-99 0.6046631110646182 100-101 0.5819371399307396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3020773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.27737359964174 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5146543428608 37.18361022187756 2 14.565918054072599 14.938040437608946 3 5.238403873920788 8.058347775265405 4 2.595795227554808 5.324222466859798 5 1.3837039334416825 3.5476351773191475 6 0.870617666462302 2.6785792407381446 7 0.5777496055847159 2.0737837660829195 8 0.41464903715353424 1.7009690872682801 9 0.3081879997399369 1.422276408143193 >10 1.4100349707109556 12.617078783430498 >50 0.08148373785046659 2.835820964857141 >100 0.03485598362138944 3.133741135166894 >500 0.0014876728132769464 0.5113346646302295 >1k 0.002069805652891045 2.312166664745055 >5k 3.234071332642258E-4 1.2819352740387175 >10k+ 6.468142665284516E-5 0.38045793196807665 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11471 0.3797372394416926 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9800 0.32442027255937467 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8012 0.26523012487201125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7786 0.2577485961374787 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6918 0.2290142291393627 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6135 0.20309371144405755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4717 0.15615208425128269 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4679 0.15489412809237899 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4411 0.14602222676116344 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4098 0.13566064050493035 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3511 0.11622852826081272 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.310410944483416E-5 0.0 0.0 0.0 5 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 0.0 6 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 0.0 7 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 0.0 8 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 0.0 9 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 0.0 10-11 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 0.0 12-13 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 6.620821888966831E-5 14-15 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 6.620821888966831E-5 16-17 3.310410944483416E-5 6.620821888966831E-5 0.0 0.0 9.931232833450246E-5 18-19 3.310410944483416E-5 6.620821888966831E-5 0.0 6.620821888966831E-5 1.3241643777933663E-4 20-21 3.310410944483416E-5 1.3241643777933663E-4 0.0 9.931232833450246E-5 1.3241643777933663E-4 22-23 3.310410944483416E-5 1.3241643777933663E-4 0.0 2.1517671139142198E-4 1.3241643777933663E-4 24-25 6.620821888966831E-5 1.3241643777933663E-4 0.0 5.296657511173464E-4 1.3241643777933663E-4 26-27 6.620821888966831E-5 1.3241643777933663E-4 0.0 0.001092435611679527 1.3241643777933663E-4 28-29 9.931232833450246E-5 1.3241643777933663E-4 0.0 0.004220773954216355 1.3241643777933663E-4 30-31 9.931232833450246E-5 1.489684925017537E-4 0.0 0.012827842409873235 1.3241643777933663E-4 32-33 9.931232833450246E-5 1.6552054722417076E-4 0.0 0.029247480694510976 1.6552054722417076E-4 34-35 1.1586438305691955E-4 1.6552054722417076E-4 0.0 0.05503558195203678 1.6552054722417076E-4 36-37 1.9862465666900493E-4 1.6552054722417076E-4 0.0 0.09530673109167753 1.9862465666900493E-4 38-39 2.3172876611383906E-4 2.3172876611383906E-4 0.0 0.15388445275431156 1.9862465666900493E-4 40-41 2.3172876611383906E-4 2.4828082083625614E-4 0.0 0.21966231822119703 1.9862465666900493E-4 42-43 2.648328755586732E-4 2.6483287555867325E-4 0.0 0.29237549461677526 1.9862465666900493E-4 44-45 3.310410944483415E-4 2.6483287555867325E-4 0.0 0.3793068860189097 2.1517671139142198E-4 46-47 3.641452038931757E-4 2.6483287555867325E-4 0.0 0.4849917554215427 2.4828082083625614E-4 48-49 3.641452038931757E-4 2.6483287555867325E-4 0.0 0.6000450877970639 2.6483287555867325E-4 50-51 3.641452038931757E-4 2.6483287555867325E-4 0.0 0.7201136927534773 2.6483287555867325E-4 52-53 3.641452038931757E-4 2.6483287555867325E-4 0.0 0.8431947716693707 2.9793698500350736E-4 54-55 3.641452038931757E-4 2.6483287555867325E-4 0.0 0.9818016779148913 2.9793698500350736E-4 56-57 3.641452038931757E-4 2.6483287555867325E-4 0.0 1.1474711936315638 3.144890397259244E-4 58-59 3.641452038931757E-4 2.9793698500350736E-4 0.0 1.335949440755727 3.310410944483415E-4 60-61 3.641452038931757E-4 2.9793698500350736E-4 0.0 1.5541882822707962 3.310410944483415E-4 62-63 3.641452038931757E-4 2.9793698500350736E-4 0.0 1.766054582717735 3.641452038931757E-4 64-65 3.641452038931757E-4 3.641452038931757E-4 0.0 1.9842272160139143 3.641452038931757E-4 66-67 3.641452038931757E-4 3.641452038931757E-4 0.0 2.239840597092201 3.641452038931757E-4 68-69 3.641452038931757E-4 3.641452038931757E-4 0.0 2.528624295834212 3.641452038931757E-4 70-71 3.8069725861559274E-4 3.641452038931757E-4 0.0 2.852299725931078 3.641452038931757E-4 72-73 3.9724931333800985E-4 3.641452038931757E-4 0.0 3.186055357353896 3.641452038931757E-4 74-75 3.9724931333800985E-4 3.9724931333800985E-4 0.0 3.5315960517390748 3.641452038931757E-4 76-77 3.9724931333800985E-4 3.9724931333800985E-4 0.0 3.891851522772482 3.641452038931757E-4 78-79 3.9724931333800985E-4 3.9724931333800985E-4 0.0 4.315335842845523 3.9724931333800985E-4 80-81 3.9724931333800985E-4 3.9724931333800985E-4 0.0 4.785496957235781 3.9724931333800985E-4 82-83 3.9724931333800985E-4 3.9724931333800985E-4 0.0 5.2559725606657635 4.30353422782844E-4 84-85 3.9724931333800985E-4 3.9724931333800985E-4 0.0 5.74948862426935 4.30353422782844E-4 86-87 4.3035342278284396E-4 3.9724931333800985E-4 0.0 6.277432961695566 4.30353422782844E-4 88-89 4.634575322276781E-4 3.9724931333800985E-4 0.0 6.836379297616869 4.30353422782844E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13980 0.0 23.70717 1 TACATGG 14210 0.0 22.982327 2 GAGTACT 9315 0.0 21.67117 12-13 ACATGGG 14210 0.0 21.650017 3 GTATAGC 955 0.0 19.859411 1 GTACTTT 10030 0.0 19.654257 14-15 AGTACTT 9795 0.0 19.280336 12-13 CATGGGG 5670 0.0 18.614902 4 TGCACCG 460 0.0 18.520538 5 CATGGGA 8760 0.0 18.478256 4 ACTTTTT 12350 0.0 16.46184 16-17 TTTGCGT 750 0.0 16.407827 3 GCACCGT 640 0.0 16.26897 6 GTGTAGC 1750 0.0 16.256346 1 AGTACAT 10755 0.0 16.238836 2 GTATAGA 850 0.0 16.176657 1 TACTTTT 11905 0.0 15.525092 14-15 TATACGT 310 2.143779E-6 15.267828 5 ATGGGAG 3775 0.0 14.920005 5 CTTTGCG 860 0.0 14.859503 2 >>END_MODULE