FastQCFastQC Report
Fri 27 May 2016
SRR939028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020773
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121310.40158595167528305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96530.31955396847098405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83810.27744554125715504No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA78810.26089348653473793No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA68140.22557140175709992No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59410.1966715142117597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.16634814996029162No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44060.14585670621393929No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT41610.13774619939995492No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40180.13301231174934364No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33650.11139532828186693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1102.757004E-630.1967743
GTACATG142500.022.3704411
TACATGG141900.021.9704112
TTTGCGT6700.021.9554373
GAGTACT99050.021.24824712-13
ACATGGG142750.020.8755873
AGTACTT105050.019.96687712-13
GTACTTT106900.019.62133414-15
CATGGGA86550.018.4764294
GTGTAGC16350.018.0691281
CATGGGG62300.017.1375874
CTTTGCG8550.016.649852
TACTTTT125300.016.49381314-15
ACTTTTT127300.016.43902816-17
TCTATCG1750.002185524216.2692818
AGTACAT111300.015.6894542
ATGGGAT37400.015.22526555
TTGCGTT10000.015.1846644
GGACCGT2551.0410565E-414.8869256
GGGTACG2256.605089E-414.8244571
Produced by FastQC (version 0.11.5)