##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939028_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3020773 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20619060088262 33.0 31.0 34.0 31.0 34.0 2 32.43122207461467 34.0 31.0 34.0 31.0 34.0 3 32.528945074654736 34.0 31.0 34.0 31.0 34.0 4 36.08241234942182 37.0 35.0 37.0 35.0 37.0 5 36.00182370538931 37.0 35.0 37.0 35.0 37.0 6 36.046583771769676 37.0 35.0 37.0 35.0 37.0 7 35.99046899584974 37.0 35.0 37.0 35.0 37.0 8 35.962230528411105 37.0 35.0 37.0 35.0 37.0 9 37.64771997101404 39.0 37.0 39.0 35.0 39.0 10-11 37.5703267011457 39.0 37.0 39.0 35.0 39.0 12-13 37.50232324640084 39.0 37.0 39.0 35.0 39.0 14-15 38.76525875992668 40.0 38.0 41.0 34.0 41.0 16-17 38.760199789921316 40.0 38.0 41.0 34.5 41.0 18-19 38.86744469048154 40.0 38.0 41.0 35.0 41.0 20-21 38.86945427544539 40.0 38.0 41.0 34.5 41.0 22-23 38.81179982739518 40.0 38.0 41.0 34.0 41.0 24-25 38.752856139802624 40.0 38.0 41.0 34.0 41.0 26-27 38.59510645122954 40.0 38.0 41.0 34.0 41.0 28-29 38.42226509572218 40.0 38.0 41.0 34.0 41.0 30-31 38.273350231877735 40.0 38.0 41.0 34.0 41.0 32-33 38.12890938842475 40.0 38.0 41.0 33.0 41.0 34-35 38.11484196263672 40.0 38.0 41.0 33.0 41.0 36-37 38.01100082660961 40.0 37.5 41.0 33.0 41.0 38-39 37.88180641180254 40.0 37.0 41.0 33.0 41.0 40-41 37.734315355705306 40.0 37.0 41.0 33.0 41.0 42-43 37.574608883222936 40.0 37.0 41.0 33.0 41.0 44-45 37.3324657297983 40.0 37.0 41.0 32.0 41.0 46-47 37.02918342424273 40.0 36.5 41.0 31.0 41.0 48-49 36.741575086906565 40.0 36.0 41.0 30.5 41.0 50-51 36.47785103349374 39.0 36.0 41.0 30.0 41.0 52-53 36.2447213014682 39.0 35.5 41.0 30.0 41.0 54-55 36.00725244829717 39.0 35.0 40.0 29.0 41.0 56-57 35.73598065793094 38.5 35.0 40.0 28.5 41.0 58-59 35.440711698628135 38.0 35.0 40.0 28.0 41.0 60-61 35.027226971374546 38.0 34.0 40.0 27.5 41.0 62-63 34.71664520968639 37.5 34.0 40.0 27.0 41.0 64-65 34.45461459699223 37.0 34.0 40.0 26.0 41.0 66-67 34.00166116421194 36.5 33.5 39.0 26.0 41.0 68-69 33.59034657685301 36.0 33.0 39.0 26.0 40.0 70-71 33.13034478261028 35.0 32.5 38.5 25.0 40.0 72-73 32.61624590791827 35.0 32.0 37.5 24.0 39.5 74-75 32.16364122693099 35.0 32.0 37.0 22.5 39.0 76-77 30.18121520551197 33.5 29.5 35.0 20.0 37.5 78-79 31.155014792571308 34.0 31.0 36.0 20.5 38.0 80-81 31.164897693404967 34.0 31.0 36.0 20.0 37.0 82-83 30.856280164050723 34.0 31.0 35.0 20.0 37.0 84-85 30.47724307652379 34.0 31.0 35.0 18.5 36.5 86-87 29.922573626022213 34.0 30.0 35.0 12.5 36.0 88-89 29.361330361467083 34.0 29.5 35.0 7.0 35.5 90-91 28.912721512010336 34.0 29.0 35.0 2.0 35.0 92-93 28.58566433161313 34.0 29.0 35.0 2.0 35.0 94-95 28.21813208076211 33.5 29.0 35.0 2.0 35.0 96-97 27.627885147278526 33.0 27.5 35.0 2.0 35.0 98-99 26.7840557698311 33.0 26.0 35.0 2.0 35.0 100-101 22.558383235019647 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.162715730660274 1101 2 0.1304671885230846 1101 3 0.1258043204304542 1101 4 0.10984647458779051 1101 5 0.1368873358710232 1101 6 0.10781902010167954 1101 7 0.1074296515721116 1101 8 0.17708271587753188 1101 9 0.10528682741094286 1101 10-11 0.21128444476732255 1101 12-13 0.27546243327803666 1101 14-15 0.28873387117439364 1101 16-17 0.2895040011836514 1101 18-19 0.3513345482733641 1101 20-21 0.2573692555100564 1101 22-23 0.3020552525187199 1101 24-25 0.33409852699869447 1101 26-27 0.30009198352426836 1101 28-29 0.30230785413266403 1101 30-31 0.2913392195884654 1101 32-33 0.40676789412035674 1101 34-35 0.4640940010397756 1101 36-37 0.45307347031814516 1101 38-39 0.3742640611857482 1101 40-41 0.38176925410900253 1101 42-43 0.45869995426409105 1101 44-45 0.4986473386660535 1101 46-47 0.592302235787848 1101 48-49 0.3822233258431069 1101 50-51 0.3581964980477892 1101 52-53 0.5159443823999013 1101 54-55 0.47112757015274553 1101 56-57 0.40634057739519847 1101 58-59 0.4273935806115503 1101 60-61 0.5965081468342177 1101 62-63 0.45758651689315855 1101 64-65 0.3266610961767995 1101 66-67 0.5001293884058455 1101 68-69 0.6163552987007606 1101 70-71 0.5381682064984616 1101 72-73 0.5012027681752116 1101 74-75 0.4648856607543408 1101 76-77 0.3236578991612724 1101 78-79 0.5059779347471434 1101 80-81 0.7458264499009317 1101 82-83 0.683910657195753 1101 84-85 0.5915586067152567 1101 86-87 0.7445035723099558 1101 88-89 0.8009385764109815 1101 90-91 1.0243305768156041 1101 92-93 1.074314717207482 1101 94-95 1.0711538808529042 1101 96-97 0.8956218223364836 1101 98-99 0.7609543214697574 1101 100-101 1.1404098560595344 1102 1 0.016407212072806487 1102 2 -0.032119169189755326 1102 3 0.01857615755132258 1102 4 -0.00901846182182453 1102 5 0.03191728851295039 1102 6 -0.02078747435535888 1102 7 0.006157078705200547 1102 8 0.04723080867589147 1102 9 0.05577277682935744 1102 10-11 0.10725148538508478 1102 12-13 -0.024680826803724187 1102 14-15 0.06131298618795 1102 16-17 0.06331647902838 1102 18-19 0.06994490635343453 1102 20-21 0.06484421846773358 1102 22-23 0.03311062091453465 1102 24-25 -0.05414420033662992 1102 26-27 -0.06668895542538422 1102 28-29 0.01616614715085518 1102 30-31 -0.07199289387709484 1102 32-33 0.017958884610486336 1102 34-35 -0.10902531988065789 1102 36-37 0.011860343690770492 1102 38-39 0.006826306320512288 1102 40-41 -0.018739201232364167 1102 42-43 -0.07264359195529124 1102 44-45 -0.055749923389598166 1102 46-47 -0.012733671679015401 1102 48-49 -0.0168964566718941 1102 50-51 0.021112730109997813 1102 52-53 -0.025345379126569867 1102 54-55 -0.060219673923676 1102 56-57 -0.08638235875428535 1102 58-59 -0.18463605835008678 1102 60-61 -0.1644312618656869 1102 62-63 -0.04502742173261254 1102 64-65 -0.010616681742668277 1102 66-67 0.009703140251595244 1102 68-69 -0.08234171674607893 1102 70-71 -0.03473504481045353 1102 72-73 -0.0035768395459143676 1102 74-75 0.19273294690982112 1102 76-77 -0.05924250862309677 1102 78-79 -0.03644439112841624 1102 80-81 0.057018077952619706 1102 82-83 0.058747505193426974 1102 84-85 0.1443219897698924 1102 86-87 0.10282304930546005 1102 88-89 0.20730373596713392 1102 90-91 0.2214713682590208 1102 92-93 0.14877859169456187 1102 94-95 -0.029951650886186343 1102 96-97 -0.1408810632671198 1102 98-99 0.02891105144204076 1102 100-101 0.6741384563058865 1103 1 0.04182017996550513 1103 2 0.020975590237561903 1103 3 0.041943957051557845 1103 4 0.014525894496500769 1103 5 0.04630623309068227 1103 6 0.03584053961717615 1103 7 0.09005346464818587 1103 8 0.10115751579155585 1103 9 0.0647866440778202 1103 10-11 0.11171306496431299 1103 12-13 0.022264534654929946 1103 14-15 0.1438709917064358 1103 16-17 0.06722807851229362 1103 18-19 0.10527589431779205 1103 20-21 0.15572020568137646 1103 22-23 0.17129069017913423 1103 24-25 0.13859398160162328 1103 26-27 0.11951170258205934 1103 28-29 0.17184041788834037 1103 30-31 0.1388689638071483 1103 32-33 0.18859768512059816 1103 34-35 0.07524729281760045 1103 36-37 0.008343349318280957 1103 38-39 0.10973301466970753 1103 40-41 0.18333612012409617 1103 42-43 0.11260874287467715 1103 44-45 0.020015130197329256 1103 46-47 0.0718134949703142 1103 48-49 0.032147463861342374 1103 50-51 -0.020442664435925906 1103 52-53 -0.07406459460126769 1103 54-55 0.02551492143165035 1103 56-57 -0.03571129955666663 1103 58-59 0.018852153647671344 1103 60-61 0.059930027958486676 1103 62-63 -0.008899872202945858 1103 64-65 -0.07594887208760781 1103 66-67 0.11350322360834042 1103 68-69 0.10669712014694 1103 70-71 0.04086952523257281 1103 72-73 0.0010020500151171063 1103 74-75 0.013470834794368614 1103 76-77 -0.038809701450677636 1103 78-79 0.174328315953602 1103 80-81 0.16469269258341157 1103 82-83 0.17829497399538852 1103 84-85 0.18077767083127583 1103 86-87 0.1792821817598309 1103 88-89 0.33503474411653755 1103 90-91 0.2526277281981919 1103 92-93 0.357383587243266 1103 94-95 0.3659396339727259 1103 96-97 -0.008877741457123278 1103 98-99 0.08633805666086758 1103 100-101 0.5166552569268887 1104 1 -0.03874017574430866 1104 2 0.0064176415497811945 1104 3 -0.07363930458585344 1104 4 0.02935371398455544 1104 5 -0.020412765470503302 1104 6 -0.048942767121261 1104 7 -0.050543216994960005 1104 8 0.018593105891881123 1104 9 -0.012563327611481157 1104 10-11 0.02691210999915228 1104 12-13 0.02155927965031168 1104 14-15 -0.022852173797161868 1104 16-17 -0.03270659853740909 1104 18-19 0.05948150351252224 1104 20-21 0.06241776900051832 1104 22-23 -0.03937386360261286 1104 24-25 -0.021405270799881748 1104 26-27 0.015261605149142099 1104 28-29 -0.02401886691727384 1104 30-31 -0.11085718228585506 1104 32-33 -0.011119636859042714 1104 34-35 0.07421811931838107 1104 36-37 0.08422821179692619 1104 38-39 0.2074767379062905 1104 40-41 0.11953226545725215 1104 42-43 0.023634533797114443 1104 44-45 0.03487542732483462 1104 46-47 0.024272726341017403 1104 48-49 -0.0017885441587992545 1104 50-51 -0.03987023684880597 1104 52-53 -0.08546988367677244 1104 54-55 -0.06882030418376672 1104 56-57 -0.03738653296672112 1104 58-59 0.0031824892336018706 1104 60-61 0.16002494845567838 1104 62-63 0.15347465993521325 1104 64-65 -0.025165057962659887 1104 66-67 0.056144158179989745 1104 68-69 -0.035374781670093114 1104 70-71 -0.11934009648258836 1104 72-73 -0.2059061890104985 1104 74-75 -0.18787539400018005 1104 76-77 -0.2651543982811084 1104 78-79 -0.04011314218210771 1104 80-81 0.03625318800835231 1104 82-83 0.07537915603684908 1104 84-85 0.01692007486291658 1104 86-87 0.0047179949141344935 1104 88-89 0.07704530608999605 1104 90-91 0.12260852349592 1104 92-93 0.17013663707744442 1104 94-95 0.12063387844478868 1104 96-97 -0.01844056791867743 1104 98-99 0.08893185576031826 1104 100-101 0.6895692732956178 1105 1 -0.060255685216866084 1105 2 -0.011153237565636687 1105 3 -0.018062018463140816 1105 4 -0.010235159460250998 1105 5 0.036130682964639504 1105 6 0.030895652111304628 1105 7 0.01984808465733323 1105 8 0.0475575067269034 1105 9 -0.04402926772787907 1105 10-11 -0.042302546515124106 1105 12-13 -0.023047757551317716 1105 14-15 0.034584791388340363 1105 16-17 0.035169084319043975 1105 18-19 -5.208320304674885E-4 1105 20-21 0.053976035237276676 1105 22-23 -0.031228687007633482 1105 24-25 0.010806824425905859 1105 26-27 -0.019050822353186447 1105 28-29 0.01453391990373376 1105 30-31 0.03204864729571 1105 32-33 0.00907825485789715 1105 34-35 0.10427356420974121 1105 36-37 0.03327001210066527 1105 38-39 0.13553018232344272 1105 40-41 0.02248832459405037 1105 42-43 0.046112833597547365 1105 44-45 0.07485604255465717 1105 46-47 0.0571060357746731 1105 48-49 -0.007044994824418893 1105 50-51 0.029279760382614484 1105 52-53 0.0427976876756091 1105 54-55 0.15306615908479415 1105 56-57 0.0865343728169492 1105 58-59 0.0049553249046141445 1105 60-61 0.012695075133450473 1105 62-63 -0.06793712255971229 1105 64-65 -0.06845276183756965 1105 66-67 -0.04403490011582534 1105 68-69 -0.09365616277818134 1105 70-71 0.10523738793647652 1105 72-73 0.0022397329705299285 1105 74-75 0.1509943238747482 1105 76-77 -0.03936992086299185 1105 78-79 -0.09747125025504033 1105 80-81 -0.05936978673039306 1105 82-83 0.10870199480771348 1105 84-85 -0.010577512689184942 1105 86-87 -0.05780208909915885 1105 88-89 0.04618981349393181 1105 90-91 0.3109213788261904 1105 92-93 0.23993955700310465 1105 94-95 0.20282955151420268 1105 96-97 0.10288704510906399 1105 98-99 0.23848658815725798 1105 100-101 0.9088827940308377 1106 1 -0.08814924210140163 1106 2 -0.20373666226911524 1106 3 -0.13837489722908458 1106 4 -0.03420769384216982 1106 5 -0.0939981359623232 1106 6 -0.009045380251549773 1106 7 0.004261835603870168 1106 8 0.009185044894969963 1106 9 -0.08321999378969736 1106 10-11 -0.04610928662634706 1106 12-13 -0.032230039061666105 1106 14-15 -0.007749363862878056 1106 16-17 -0.040341289533792235 1106 18-19 -0.12774863196924713 1106 20-21 -0.0654818889399067 1106 22-23 -0.013434266780500081 1106 24-25 0.02106852937149739 1106 26-27 0.029436294542641406 1106 28-29 -0.041138680716983345 1106 30-31 0.007235234500441834 1106 32-33 0.08352737738719185 1106 34-35 0.0552990308955259 1106 36-37 0.02511119545934548 1106 38-39 0.10589054510571572 1106 40-41 0.1431178019283479 1106 42-43 0.2181467728717692 1106 44-45 0.1320290250204721 1106 46-47 0.16345927139703775 1106 48-49 0.036537671034750474 1106 50-51 0.23341280239295514 1106 52-53 0.2901830669368124 1106 54-55 0.23256326348859346 1106 56-57 0.18760465827602246 1106 58-59 0.21696877526711233 1106 60-61 0.2956297200278897 1106 62-63 0.20706859732878513 1106 64-65 0.16369463833321163 1106 66-67 0.23923601537900652 1106 68-69 0.15892909413567935 1106 70-71 0.09905448557447727 1106 72-73 0.25726461030300385 1106 74-75 0.35084122816032703 1106 76-77 0.08939968179772606 1106 78-79 0.25146508124212374 1106 80-81 0.3178720028499349 1106 82-83 0.3539065475966616 1106 84-85 0.3046695120746179 1106 86-87 0.3320413965218556 1106 88-89 0.35231345746499443 1106 90-91 0.49899575456799994 1106 92-93 0.456125093455956 1106 94-95 0.6055646793137619 1106 96-97 0.5241129023048821 1106 98-99 0.40901956869394596 1106 100-101 0.8020886395203561 1107 1 -0.13607697606503422 1107 2 -0.09698356342596526 1107 3 -0.07506364916148556 1107 4 -0.12643203297053418 1107 5 -0.15881699135545801 1107 6 -0.09512032882403787 1107 7 -0.10210053804138397 1107 8 -0.04266359233905348 1107 9 -0.03478394997524248 1107 10-11 -0.04056921400606228 1107 12-13 -0.06116704856159316 1107 14-15 -0.04728201247495889 1107 16-17 -0.03347170731837679 1107 18-19 -0.04255252830019174 1107 20-21 -0.007790843131679992 1107 22-23 -0.09370999762933963 1107 24-25 -0.15122390331615776 1107 26-27 -0.14766139272209955 1107 28-29 -0.1007965449391719 1107 30-31 -0.1566267800814387 1107 32-33 -0.035356786701782994 1107 34-35 -0.19571070580203553 1107 36-37 -0.07804260847873934 1107 38-39 -0.023523295973063796 1107 40-41 -0.2081185117916391 1107 42-43 -0.17898730483550906 1107 44-45 -0.18914404765808968 1107 46-47 -0.27006123376039426 1107 48-49 -0.16148171012811474 1107 50-51 -0.09943367311891649 1107 52-53 0.03930990051173211 1107 54-55 -0.08501957690693729 1107 56-57 0.014379004952751018 1107 58-59 -0.04618820173585192 1107 60-61 -0.0077943221217964265 1107 62-63 -0.12129750139827422 1107 64-65 -0.10464673205454744 1107 66-67 0.04088997831448893 1107 68-69 0.014137073281091261 1107 70-71 0.1984743745195061 1107 72-73 -0.04412097283198335 1107 74-75 -0.12696595916430198 1107 76-77 -0.33371280346690924 1107 78-79 -0.060543099437651904 1107 80-81 0.09591261578603749 1107 82-83 0.06537698211152687 1107 84-85 -0.02319590546447614 1107 86-87 -0.017878829460936885 1107 88-89 0.13037988166636794 1107 90-91 0.24813153604822347 1107 92-93 0.24735474672233337 1107 94-95 0.21098023839035562 1107 96-97 0.04328126563205004 1107 98-99 -0.04947660504408802 1107 100-101 0.5186380427409603 1108 1 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44.0 6 128.0 7 309.0 8 785.0 9 1444.0 10 2571.0 11 3951.0 12 5732.0 13 8067.0 14 9903.0 15 13283.0 16 17398.0 17 24630.0 18 23775.0 19 16214.0 20 14944.0 21 15653.0 22 16875.0 23 18643.0 24 21789.0 25 25870.0 26 31534.0 27 38657.0 28 47143.0 29 57505.0 30 70431.0 31 86060.0 32 106986.0 33 137628.0 34 185459.0 35 268646.0 36 434340.0 37 705353.0 38 561931.0 39 47056.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25614048150576 19.463295390633416 14.608349432720956 22.67221469513987 2 15.41175056848032 21.058285412376236 39.71890638588202 23.81105763326142 3 19.932911211799098 24.99022601168641 30.098785972994328 24.978076803520157 4 13.042522559622984 16.852871764942286 37.22550486249712 32.879100812937615 5 13.450352411197047 38.035680412982025 34.17579212655354 14.338175049267388 6 31.401068534444658 36.25869934616073 17.358669453149904 14.981562666244699 7 28.03699582855117 33.29819883850922 20.727178109709005 17.93762722323061 8 25.76271040558162 34.97224054902504 19.687874593688438 19.577174451704913 9 26.16611708327637 17.318183127298873 20.77521217251346 35.740487616911295 10-11 24.976487806266807 26.486664175030693 27.102003361391276 21.434844657311224 12-13 26.45377524229725 24.00196903242978 27.460736043390217 22.08351968188275 14-15 23.075304773032627 25.324114374283184 26.572934403471475 25.027646449212714 16-17 21.883432982149948 28.554703451179968 27.190760791030794 22.371102775639287 18-19 21.69641677808958 27.7846432022532 29.054417528228704 21.464522491428518 20-21 23.02965168187083 26.445780599866325 28.616979826024664 21.90758789223818 22-23 22.427918284492083 26.403539756214716 28.570865801568008 22.597676157725193 24-25 22.576770912610776 26.434442442381467 28.938834530102064 22.04995211490569 26-27 22.498214860898187 26.27646963211072 29.27864821355329 21.946667293437805 28-29 21.949183205755613 26.999645454987846 29.11039657729992 21.940774761956625 30-31 22.213056062140385 26.733571175324993 28.804630470412707 22.248742292121918 32-33 22.085539032558884 26.733041509573873 28.797033077295115 22.384386380572124 34-35 22.724658196570307 26.484260051170168 28.65424981018507 22.13683194207446 36-37 22.716361115451743 26.271886727988793 29.021417277371818 21.990334879187646 38-39 22.521984289805754 26.57363911448021 29.061756978514904 21.842619617199137 40-41 22.42672970726312 26.646696675173526 28.83789480514278 22.08867881242057 42-43 22.04109399711827 26.91419288042817 28.38670432830176 22.658008794151797 44-45 22.41743228449236 26.810593879758194 28.182167882750154 22.589805952999292 46-47 22.697606085348955 26.983956208422143 27.777546967844387 22.540890738384515 48-49 22.65727015221804 26.884195413381146 27.916450316980914 22.542084117419904 50-51 22.444826688965737 27.271221026273572 28.009794905510343 22.274157379250344 52-53 22.62424220555467 27.383222771125137 27.61614328517899 22.376391738141198 54-55 22.123476341982666 26.96270126884741 27.88772939906441 23.02609299010551 56-57 22.584633138603927 27.323982967273608 27.36582656161188 22.725557332510586 58-59 22.87189880189409 26.900956975179824 27.506382482865284 22.720761740060805 60-61 22.563312631209044 26.842711691924848 28.12827272578301 22.4657029510831 62-63 22.560798179803644 26.808320254451427 28.024300402579076 22.60658116316585 64-65 22.35471069258592 27.15400663275474 27.863859696514066 22.62742297814528 66-67 22.23366538932452 27.036319586139157 27.566175741556126 23.1638392829802 68-69 22.069582290744393 27.203497967624003 27.790873417660755 22.936046323970846 70-71 22.508328166333584 26.947506482612233 27.420646966852523 23.12351838420166 72-73 22.288698952221832 26.699457390542094 27.691405478001823 23.320438179234255 74-75 22.06988078879148 27.437579718833554 27.318355268667986 23.174184223706977 76-77 22.288884867143178 26.756917292136517 27.498962522191416 23.45523531852889 78-79 22.1927246069423 26.856500069601424 27.693190299233162 23.257585024223115 80-81 22.23520271135898 27.0558727848799 27.46964104883088 23.239283454930245 82-83 22.186357597873126 26.974569092083385 27.54948816081182 23.28958514923167 84-85 22.1017931502963 26.789252287411202 27.39429609573444 23.714658466558063 86-87 22.04728246896463 26.797261457959838 27.58405935264887 23.57139672042666 88-89 22.16605174149734 26.60159299756734 27.3806349113457 23.851720349589613 90-91 22.029133253810155 26.468197537704587 27.63458733482082 23.86808187366444 92-93 22.09489094347705 26.7900633380926 27.476791536470962 23.638254181959386 94-95 21.873407899236387 26.706409253525504 27.42096145589225 23.999221391345856 96-97 21.426290780941276 26.49020105380818 27.676111810990516 24.40739635426003 98-99 21.66066433988916 26.540359040550214 27.153860948836606 24.645115670724017 100-101 21.87864352466424 25.983027506231853 27.833052499859306 24.305276469244596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 504.0 1 355.0 2 244.5 3 304.0 4 309.0 5 325.0 6 517.0 7 1032.0 8 1746.0 9 2228.5 10 2279.5 11 2285.5 12 2486.0 13 2727.5 14 3019.0 15 3240.0 16 3464.0 17 3796.5 18 4166.5 19 4608.0 20 5211.0 21 6080.5 22 7333.0 23 8922.5 24 10694.5 25 13293.0 26 17189.0 27 21642.5 28 25484.0 29 29383.0 30 33724.0 31 38591.5 32 44143.0 33 49186.5 34 55376.0 35 61142.0 36 67179.5 37 73033.5 38 76351.5 39 78953.5 40 81614.5 41 85365.0 42 88563.5 43 92602.5 44 97828.5 45 102003.0 46 106985.0 47 121129.5 48 157836.5 49 157817.0 50 125071.0 51 115375.0 52 109969.0 53 104987.5 54 101211.0 55 95206.0 56 86448.5 57 77936.5 58 70528.5 59 62231.0 60 52597.0 61 42702.5 62 33506.0 63 25782.5 64 18731.0 65 13242.0 66 9290.5 67 6882.0 68 5014.5 69 3353.0 70 2293.5 71 1494.0 72 1014.5 73 722.5 74 477.0 75 301.0 76 171.0 77 85.0 78 37.5 79 17.0 80 9.5 81 5.0 82 6.0 83 5.5 84 4.5 85 3.0 86 4.5 87 4.5 88 1.0 89 2.0 90 3.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4097295625987123 2 0.0 3 0.0 4 0.0 5 3.9724931333800985E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013738205419606174 16-17 0.0026979849197539835 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022345273875263054 36-37 0.007316008187308348 38-39 0.012496801315424891 40-41 0.0024497040989177273 42-43 5.131136963949294E-4 44-45 3.310410944483415E-4 46-47 3.1448903972592447E-4 48-49 2.4828082083625614E-4 50-51 6.289780794518489E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6552054722417076E-4 60-61 0.0022676314969711396 62-63 0.0 64-65 2.3172876611383906E-4 66-67 0.0015724451986296223 68-69 0.0015724451986296223 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.127152884377608 78-79 4.965616416725123E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1488526281186968 88-89 0.4423040062924291 90-91 0.3419654505651368 92-93 0.0 94-95 0.0 96-97 4.8000958695009523E-4 98-99 0.0 100-101 9.931232833450246E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3020773.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.64328557541928 #Duplication Level Percentage of deduplicated Percentage of total 1 70.76752811010797 35.131326074366015 2 15.422985651342403 15.312953622303697 3 5.711922838695265 8.506758499983256 4 2.6720228603902405 5.305919756896055 5 1.5003543972764206 3.724126090416471 6 0.9186837725259276 2.7363888523804887 7 0.6024502898579367 2.093532824908817 8 0.4494467143324381 1.7849609272431293 9 0.3180852599423351 1.4211717656983935 >10 1.5084795152701138 13.15767913202332 >50 0.08595820960752336 2.918744302152358 >100 0.03794815159957428 3.295530562694282 >500 0.001400303388822033 0.44910427287892457 >1k 0.0022671578668886556 2.3138122250770365 >5k 4.0008668239211565E-4 1.4464222892606526 >10k+ 6.668111373201928E-5 0.40156880171692366 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12131 0.40158595167528305 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9653 0.31955396847098405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8381 0.27744554125715504 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7881 0.26089348653473793 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6814 0.22557140175709992 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5941 0.1966715142117597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5025 0.16634814996029162 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4406 0.14585670621393929 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4161 0.13774619939995492 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4018 0.13301231174934364 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3365 0.11139532828186693 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.310410944483416E-5 0.0 0.0 0.0 0.0 7 3.310410944483416E-5 3.310410944483416E-5 0.0 0.0 0.0 8 3.310410944483416E-5 3.310410944483416E-5 0.0 0.0 0.0 9 3.310410944483416E-5 3.310410944483416E-5 0.0 0.0 0.0 10-11 3.310410944483416E-5 3.310410944483416E-5 0.0 3.310410944483416E-5 0.0 12-13 3.310410944483416E-5 6.620821888966831E-5 0.0 3.310410944483416E-5 1.655205472241708E-5 14-15 9.931232833450246E-5 9.931232833450246E-5 0.0 6.620821888966831E-5 3.310410944483416E-5 16-17 9.931232833450246E-5 9.931232833450246E-5 0.0 6.620821888966831E-5 3.310410944483416E-5 18-19 1.1586438305691955E-4 9.931232833450246E-5 0.0 1.324164377793366E-4 3.310410944483416E-5 20-21 1.3241643777933663E-4 1.6552054722417076E-4 0.0 1.6552054722417076E-4 8.276027361208538E-5 22-23 1.3241643777933663E-4 1.6552054722417076E-4 0.0 2.813849302810903E-4 9.931232833450246E-5 24-25 1.3241643777933663E-4 1.6552054722417076E-4 0.0 5.958739700070148E-4 9.931232833450246E-5 26-27 1.3241643777933663E-4 1.6552054722417076E-4 0.0 0.0011420917758467783 1.6552054722417076E-4 28-29 2.3172876611383906E-4 1.6552054722417076E-4 0.0 0.004237326008938772 1.6552054722417076E-4 30-31 2.3172876611383906E-4 1.6552054722417076E-4 0.0 0.01291060268348532 1.6552054722417076E-4 32-33 2.3172876611383906E-4 1.9862465666900493E-4 0.0 0.029247480694510976 1.8207260194658784E-4 34-35 2.813849302810903E-4 1.9862465666900493E-4 0.0 0.05551559153898687 1.9862465666900493E-4 36-37 3.310410944483415E-4 2.1517671139142198E-4 0.0 0.09648192697696914 1.9862465666900493E-4 38-39 3.641452038931757E-4 2.3172876611383906E-4 0.0 0.15608587603239304 2.3172876611383906E-4 40-41 3.8069725861559274E-4 2.3172876611383906E-4 0.0 0.2228568647826235 2.3172876611383906E-4 42-43 4.3035342278284396E-4 2.3172876611383906E-4 0.0 0.29628177953126567 2.4828082083625614E-4 44-45 5.296657511173465E-4 2.3172876611383906E-4 0.0 0.3842725024356348 2.6483287555867325E-4 46-47 5.958739700070148E-4 2.3172876611383906E-4 0.0 0.4909835992310577 2.813849302810903E-4 48-49 6.62082188896683E-4 2.3172876611383906E-4 0.0 0.6070962631088135 3.310410944483415E-4 50-51 6.62082188896683E-4 2.3172876611383906E-4 0.0 0.7286380009355221 3.310410944483415E-4 52-53 6.62082188896683E-4 2.4828082083625614E-4 0.0 0.8529604839555969 3.641452038931757E-4 54-55 6.62082188896683E-4 2.6483287555867325E-4 0.0 0.9922791285541813 3.641452038931757E-4 56-57 6.951862983415173E-4 2.6483287555867325E-4 0.0 1.158627278514473 3.641452038931757E-4 58-59 6.951862983415173E-4 2.9793698500350736E-4 0.0 1.3483469297428174 3.641452038931757E-4 60-61 6.951862983415173E-4 2.9793698500350736E-4 0.0 1.5679926959092922 3.641452038931757E-4 62-63 6.951862983415173E-4 2.9793698500350736E-4 0.0 1.7817624826493086 3.641452038931757E-4 64-65 6.951862983415173E-4 2.9793698500350736E-4 0.0 2.002268955661349 3.641452038931757E-4 66-67 7.613945172311855E-4 2.9793698500350736E-4 0.0 2.259752718923269 3.641452038931757E-4 68-69 7.613945172311855E-4 2.9793698500350736E-4 0.0 2.5502412793016886 3.641452038931757E-4 70-71 7.779465719536026E-4 2.9793698500350736E-4 0.0 2.8757705395274655 3.641452038931757E-4 72-73 8.276027361208539E-4 3.310410944483415E-4 0.0 3.211578625735863 3.641452038931757E-4 74-75 8.60706845565688E-4 3.310410944483415E-4 0.0 3.5606780118863615 3.641452038931757E-4 76-77 8.60706845565688E-4 3.310410944483415E-4 0.0 3.9232176664714626 3.641452038931757E-4 78-79 8.60706845565688E-4 3.310410944483415E-4 0.0 4.349598596120927 3.9724931333800985E-4 80-81 9.600191739001905E-4 3.4759314917075864E-4 0.0 4.822341831047881 3.9724931333800985E-4 82-83 9.600191739001905E-4 3.641452038931757E-4 0.0 5.295217482412614 3.9724931333800985E-4 84-85 9.765712286226075E-4 3.641452038931757E-4 0.0 5.79111704189623 3.9724931333800985E-4 86-87 9.931232833450246E-4 3.641452038931757E-4 0.0 6.320203471098292 4.1380136806042696E-4 88-89 9.931232833450246E-4 3.641452038931757E-4 0.0 6.88083811660128 4.30353422782844E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 110 2.757004E-6 30.196774 3 GTACATG 14250 0.0 22.370441 1 TACATGG 14190 0.0 21.970411 2 TTTGCGT 670 0.0 21.955437 3 GAGTACT 9905 0.0 21.248247 12-13 ACATGGG 14275 0.0 20.875587 3 AGTACTT 10505 0.0 19.966877 12-13 GTACTTT 10690 0.0 19.621334 14-15 CATGGGA 8655 0.0 18.476429 4 GTGTAGC 1635 0.0 18.069128 1 CATGGGG 6230 0.0 17.137587 4 CTTTGCG 855 0.0 16.64985 2 TACTTTT 12530 0.0 16.493813 14-15 ACTTTTT 12730 0.0 16.439028 16-17 TCTATCG 175 0.0021855242 16.269281 8 AGTACAT 11130 0.0 15.689454 2 ATGGGAT 3740 0.0 15.2252655 5 TTGCGTT 1000 0.0 15.184664 4 GGACCGT 255 1.0410565E-4 14.886925 6 GGGTACG 225 6.605089E-4 14.824457 1 >>END_MODULE