FastQCFastQC Report
Fri 27 May 2016
SRR939027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607767
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142120.39392787838017257No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT132080.3660990302311652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109090.30237540284613723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69540.19275080680099352No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68380.18953552155668588No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55630.15419510184554602No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50040.13870075312513253No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37530.1040255648438494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG154150.024.3249781
TACATGG158100.023.3969382
ACATGGG155300.022.4768733
GAGTACT118000.021.80603212-13
GTACTTT127000.020.1493114-15
CATGGGG65750.020.097794
AGTACTT122250.019.75060712-13
AATTCGC3700.018.63398678-79
GTATCAA334800.018.4584121
GTATAGG8100.018.1654241
TATCACG1851.5564966E-417.9211642
TCGCGCT3900.017.68492560-61
TTTGCGT7300.017.5178493
CATGGGA98050.017.4381124
AGAGTAC234800.017.43090810-11
CGCGCTT4100.017.40268762-63
AGTACAT117800.017.3289342
ACTTTTT158850.016.61735716-17
GGTATCA271300.016.497981
CCGCGAT4450.016.4949622-23
Produced by FastQC (version 0.11.5)