FastQCFastQC Report
Fri 27 May 2016
SRR939027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607767
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139590.38691523039043263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137760.3818428407377749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116870.32393998836399357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.2012325075316671No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66020.18299407916309451No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51530.14283073158549317No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48940.13565177573828907No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38500.10671420853952042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG153600.024.1639561
TACATGG155950.023.0965612
ACATGGG154850.022.065423
GAGTACT132350.021.4621512-13
TTTGCGT7400.020.5215033
AGTACTT137050.020.0854712-13
GTACTTT143200.019.70343214-15
CATGGGG64400.018.643424
CATGGGA101450.017.728754
AGAGTAC253450.017.47118810-11
CTTTGCG8700.017.4550722
GTATCAA363950.017.188771
GTATAGG8650.017.075261
CGATAGT1952.2931922E-417.0354796
TTGCGTT8950.016.96754
CGCGGAA3950.016.91749482-83
GTAAGCG4500.016.87358794-95
AGTACAT118050.016.6025562
TACTTTT164700.016.46856314-15
ACTTTTT173200.016.1388816-17
Produced by FastQC (version 0.11.5)