##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939027_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3607767 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.192128261054556 33.0 31.0 34.0 30.0 34.0 2 32.42629554513914 34.0 31.0 34.0 31.0 34.0 3 32.517054732193074 34.0 31.0 34.0 31.0 34.0 4 36.06335470112122 37.0 35.0 37.0 35.0 37.0 5 35.98963486278355 37.0 35.0 37.0 35.0 37.0 6 36.0317578712816 37.0 35.0 37.0 35.0 37.0 7 35.96827372721132 37.0 35.0 37.0 35.0 37.0 8 35.94117192157919 37.0 35.0 37.0 35.0 37.0 9 37.61288852633776 39.0 37.0 39.0 35.0 39.0 10-11 37.52613112210406 39.0 37.0 39.0 35.0 39.0 12-13 37.43960419284283 39.0 37.0 39.0 34.0 39.0 14-15 38.74913000202064 40.0 38.0 41.0 34.5 41.0 16-17 38.84281412851772 40.0 38.0 41.0 35.0 41.0 18-19 38.88573721640006 40.0 38.0 41.0 35.0 41.0 20-21 38.860269246877635 40.0 38.0 41.0 34.5 41.0 22-23 38.78899579712326 40.0 38.0 41.0 34.0 41.0 24-25 38.71170893242274 40.0 38.0 41.0 34.0 41.0 26-27 38.552148461915635 40.0 38.0 41.0 34.0 41.0 28-29 38.394717563523365 40.0 38.0 41.0 34.0 41.0 30-31 38.20279122792575 40.0 38.0 41.0 33.5 41.0 32-33 38.115285715513224 40.0 37.5 41.0 33.0 41.0 34-35 38.01184943484432 40.0 37.0 41.0 33.0 41.0 36-37 37.87745716394656 40.0 37.0 41.0 33.0 41.0 38-39 37.743383511185726 40.0 37.0 41.0 33.0 41.0 40-41 37.57659252939561 40.0 37.0 41.0 33.0 41.0 42-43 37.39159097580304 40.0 37.0 41.0 32.0 41.0 44-45 37.134827581714674 40.0 36.5 41.0 31.0 41.0 46-47 36.81433141330912 40.0 36.0 41.0 31.0 41.0 48-49 36.47431943359979 39.0 36.0 41.0 30.0 41.0 50-51 36.21384737428997 39.0 35.5 41.0 30.0 41.0 52-53 35.96215761716319 39.0 35.0 40.0 29.0 41.0 54-55 35.66178636258938 39.0 35.0 40.0 28.0 41.0 56-57 35.53025638850846 38.5 35.0 40.0 28.0 41.0 58-59 35.255232807440166 38.0 34.5 40.0 28.0 41.0 60-61 34.88666368975602 38.0 34.0 40.0 26.5 41.0 62-63 34.47879117470724 37.0 34.0 40.0 26.0 41.0 64-65 34.0628678348685 37.0 33.5 39.5 26.0 41.0 66-67 33.61133119184249 36.0 33.0 39.0 25.0 40.5 68-69 33.120517483529284 36.0 32.5 39.0 23.5 40.0 70-71 32.584821442182935 35.0 32.0 38.0 22.5 40.0 72-73 32.07338514377453 35.0 31.0 37.0 21.5 39.5 74-75 31.54706110455581 35.0 31.0 37.0 20.0 39.0 76-77 29.65493142988447 32.5 28.5 35.0 18.0 37.0 78-79 30.614803145546816 34.0 30.0 36.0 18.0 37.5 80-81 30.589485546045516 34.0 30.0 35.5 18.0 37.0 82-83 30.177166790427428 34.0 30.0 35.0 14.0 37.0 84-85 29.73091097623544 34.0 30.0 35.0 7.5 36.0 86-87 29.044380083303608 34.0 29.0 35.0 3.5 36.0 88-89 28.3513850811319 33.0 28.5 35.0 2.0 35.0 90-91 27.76923509749937 33.0 26.5 35.0 2.0 35.0 92-93 27.360125667760695 33.0 26.0 34.5 2.0 35.0 94-95 26.80458383260338 32.5 25.5 34.0 2.0 35.0 96-97 25.942591913502177 32.0 24.5 34.0 2.0 35.0 98-99 24.734933547537853 32.0 11.0 34.0 2.0 35.0 100-101 19.18919777801615 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.021685797200412082 1101 2 0.07221596392101759 1101 3 0.15613342104011707 1101 4 0.06407876466941076 1101 5 0.052096687598123026 1101 6 0.05181732328838251 1101 7 0.09530435302119855 1101 8 0.1383101119612462 1101 9 0.17613124300140015 1101 10-11 0.2391123765532086 1101 12-13 0.18510284117020603 1101 14-15 0.22952526814370344 1101 16-17 0.1853304208765607 1101 18-19 0.2367800535300475 1101 20-21 0.25130466864291634 1101 22-23 0.23136298297762892 1101 24-25 0.1902302235428337 1101 26-27 0.11991034297972902 1101 28-29 0.1772120266453996 1101 30-31 0.11116903464633054 1101 32-33 0.22361395925726413 1101 34-35 0.3134902990695636 1101 36-37 0.31910615135114995 1101 38-39 0.2885912518663716 1101 40-41 0.2562057227795407 1101 42-43 0.2776496699460296 1101 44-45 0.27049658329354287 1101 46-47 0.1713541757066892 1101 48-49 0.20985415871793833 1101 50-51 0.13999521741108367 1101 52-53 0.2292207145527314 1101 54-55 0.17683101421597058 1101 56-57 0.12311779908376508 1101 58-59 0.24301065783802045 1101 60-61 0.19149994651333913 1101 62-63 0.10653293084180149 1101 64-65 0.15775021531468525 1101 66-67 0.018776104106407843 1101 68-69 0.07184281470051701 1101 70-71 0.1676143124957079 1101 72-73 0.18239481943589908 1101 74-75 0.16038457336497558 1101 76-77 -0.16557232537703115 1101 78-79 0.3111066211163056 1101 80-81 0.43776012195197467 1101 82-83 0.3576561937081628 1101 84-85 0.47306526927365056 1101 86-87 0.5915540832467165 1101 88-89 0.6001220866929629 1101 90-91 0.6575236078990834 1101 92-93 0.47119893670763346 1101 94-95 0.3687072378909022 1101 96-97 0.36213561758215107 1101 98-99 0.4796720911467034 1101 100-101 0.9312664537869608 1102 1 0.023198055815782936 1102 2 0.052614957696825115 1102 3 0.11119557130251678 1102 4 0.10501193795847286 1102 5 0.08680984309548023 1102 6 0.0712667678557537 1102 7 0.10881321592941617 1102 8 0.07955764409276611 1102 9 0.15276651613638137 1102 10-11 0.19730445654001016 1102 12-13 0.15396472566425246 1102 14-15 0.2504222626669801 1102 16-17 0.1781941811049279 1102 18-19 0.06047069241052583 1102 20-21 0.15091276831567768 1102 22-23 0.17633003782969325 1102 24-25 0.2456621751723489 1102 26-27 0.2235629970603057 1102 28-29 0.17747762256677646 1102 30-31 0.13868360135515445 1102 32-33 0.2281829986776316 1102 34-35 0.10212935525610334 1102 36-37 0.1852684504706943 1102 38-39 0.0830273599154907 1102 40-41 0.05200992435908347 1102 42-43 0.1228558473450434 1102 44-45 0.15259019067696755 1102 46-47 0.2883589125983832 1102 48-49 0.30059017479545247 1102 50-51 0.21785933793871948 1102 52-53 0.2167377062480611 1102 54-55 0.23226001629227966 1102 56-57 0.24176725221844464 1102 58-59 0.16124686495582807 1102 60-61 0.09778283970374702 1102 62-63 0.24210283625098583 1102 64-65 0.19892030579246267 1102 66-67 0.06119335643332846 1102 68-69 0.06352870489110529 1102 70-71 0.1523955709076077 1102 72-73 0.08764482447283228 1102 74-75 0.0029931473258812957 1102 76-77 -0.1535739059745218 1102 78-79 0.04911695961792262 1102 80-81 0.08745716199819498 1102 82-83 0.06671743895688564 1102 84-85 0.31324754077085615 1102 86-87 0.4938538544158426 1102 88-89 0.38480828781183263 1102 90-91 0.3319473877233783 1102 92-93 0.44168312084034156 1102 94-95 0.6137128728988479 1102 96-97 0.6139284921513593 1102 98-99 0.8773979220993695 1102 100-101 1.138540451306433 1103 1 0.19349674712350406 1103 2 0.13054915369963283 1103 3 0.11520552283189289 1103 4 0.11914393133285017 1103 5 0.12262691412028204 1103 6 0.10681789032950917 1103 7 0.15448092675772074 1103 8 0.13276226050879103 1103 9 0.2325050903467485 1103 10-11 0.1494568037950188 1103 12-13 0.14125791112800812 1103 14-15 0.16134440427449448 1103 16-17 0.21329402724378355 1103 18-19 0.22816479667835665 1103 20-21 0.2519602202267919 1103 22-23 0.2507672739716469 1103 24-25 0.24722350948875516 1103 26-27 0.15931997062033787 1103 28-29 0.20838973338751998 1103 30-31 0.29138915068161 1103 32-33 0.3214462292090374 1103 34-35 0.25452578256544456 1103 36-37 0.2526117862326487 1103 38-39 0.279854646418606 1103 40-41 0.2987320130313833 1103 42-43 0.432189641680111 1103 44-45 0.389419284103262 1103 46-47 0.3951558886015718 1103 48-49 0.34401940957621235 1103 50-51 0.37289480016929843 1103 52-53 0.49333492453164496 1103 54-55 0.5472859821781455 1103 56-57 0.5595409592685741 1103 58-59 0.4594669002071896 1103 60-61 0.45349457110697955 1103 62-63 0.4458896854859731 1103 64-65 0.4583357570578883 1103 66-67 0.5356530245993412 1103 68-69 0.5000669818498906 1103 70-71 0.4947645198963855 1103 72-73 0.46189033406879787 1103 74-75 0.47562853204418687 1103 76-77 0.34612078945167113 1103 78-79 0.5317914022348411 1103 80-81 0.46751309299627053 1103 82-83 0.5873590821008321 1103 84-85 0.7105039624376452 1103 86-87 0.8241221443321187 1103 88-89 0.7216537598877792 1103 90-91 0.7809637961854499 1103 92-93 0.8845144531062452 1103 94-95 1.0790039511338954 1103 96-97 0.7675965825426019 1103 98-99 0.808864543829273 1103 100-101 1.2377011773041673 1104 1 -0.021319428105329052 1104 2 0.020328175552570826 1104 3 0.03071536847650691 1104 4 0.012921163068583041 1104 5 0.030421101305748266 1104 6 0.06253993241415401 1104 7 0.05465444991102686 1104 8 0.09969575596594638 1104 9 0.07388761812734401 1104 10-11 0.1345405677697542 1104 12-13 0.13380401508991469 1104 14-15 0.11269670271742882 1104 16-17 0.09280098505781353 1104 18-19 0.1098207395297024 1104 20-21 0.14810492418194343 1104 22-23 0.037666275896867774 1104 24-25 0.05759035685593972 1104 26-27 -0.025777725870362644 1104 28-29 0.053145618820018115 1104 30-31 0.03385925910191645 1104 32-33 0.02612649907764819 1104 34-35 0.14886453691406842 1104 36-37 0.022966980950116067 1104 38-39 0.03279525168088071 1104 40-41 0.12591604045733362 1104 42-43 -0.019741067273855606 1104 44-45 0.1520018237053833 1104 46-47 0.20161424304477293 1104 48-49 0.0514302414740726 1104 50-51 0.0011541645088115615 1104 52-53 -0.05057361061206933 1104 54-55 0.15596066215248072 1104 56-57 0.040470300302793305 1104 58-59 0.15034236884635988 1104 60-61 0.12375097162037463 1104 62-63 0.11930834492462594 1104 64-65 0.17277512105964377 1104 66-67 0.1846391174152302 1104 68-69 0.17655290578679228 1104 70-71 0.21899358734665242 1104 72-73 0.18100975684043163 1104 74-75 0.23654022522485008 1104 76-77 0.06957723556238804 1104 78-79 0.3512946024915351 1104 80-81 0.34887244213667756 1104 82-83 0.21948999708715178 1104 84-85 0.42943867382346923 1104 86-87 0.428745839741989 1104 88-89 0.4589667264159658 1104 90-91 0.5182123776020084 1104 92-93 0.5658598697306942 1104 94-95 0.6389338241369025 1104 96-97 0.6367312044225599 1104 98-99 0.9039993514761164 1104 100-101 1.1904308949385083 1105 1 0.07983655381005406 1105 2 0.12702773547155743 1105 3 0.12591280320625486 1105 4 0.13141576257161347 1105 5 0.05054860240321801 1105 6 0.08622839047585273 1105 7 0.09745986722899858 1105 8 0.08993855268216322 1105 9 0.12260311970072024 1105 10-11 0.12815235012521242 1105 12-13 0.17840521047466495 1105 14-15 0.13343003317888247 1105 16-17 0.11490418391890955 1105 18-19 0.18945268451548003 1105 20-21 0.17465470373116432 1105 22-23 0.1460784063648859 1105 24-25 0.04657025405640525 1105 26-27 -0.03734498113512785 1105 28-29 0.10282738745884501 1105 30-31 0.061637065370540256 1105 32-33 0.12897966648485237 1105 34-35 0.21939869926266908 1105 36-37 0.21449901981891628 1105 38-39 0.15036632038849262 1105 40-41 0.29731677470064 1105 42-43 0.31049721991114154 1105 44-45 0.18399475102363994 1105 46-47 0.2064878018068086 1105 48-49 0.1197623747729466 1105 50-51 0.13433388659314005 1105 52-53 0.22122069048922555 1105 54-55 0.2913620292344703 1105 56-57 0.2902599523816889 1105 58-59 0.20243283913679733 1105 60-61 0.15466369697727345 1105 62-63 0.22277759840061861 1105 64-65 0.10005666521339407 1105 66-67 0.08992498716328612 1105 68-69 0.19944924708343592 1105 70-71 0.30342413167681315 1105 72-73 0.2707416072593887 1105 74-75 0.18318365019842275 1105 76-77 0.012362002714674247 1105 78-79 0.1316538076789655 1105 80-81 0.19927810335908447 1105 82-83 0.22153193606933286 1105 84-85 0.24843467234103045 1105 86-87 0.3247070827315319 1105 88-89 0.4884979163715073 1105 90-91 0.5987543059855902 1105 92-93 0.8701216416926947 1105 94-95 0.9140562122670239 1105 96-97 0.7097129156922151 1105 98-99 0.9765195110294158 1105 100-101 1.011925127593397 1106 1 0.023212320619954596 1106 2 0.0649336598640673 1106 3 0.06334185025876593 1106 4 -0.0016769678858139514 1106 5 0.04280918482695739 1106 6 0.1209582131831084 1106 7 0.08354920397759713 1106 8 0.11556809845461657 1106 9 0.045370632680523215 1106 10-11 0.12584178875025032 1106 12-13 0.1011194539075504 1106 14-15 0.08999270766275913 1106 16-17 -0.007099623412280209 1106 18-19 -0.05510910055497931 1106 20-21 1.7997488778576098E-4 1106 22-23 0.033357036928919115 1106 24-25 0.12690307048712413 1106 26-27 0.04370695328893248 1106 28-29 0.0010054686822442704 1106 30-31 0.19501444321866046 1106 32-33 0.2255688863329084 1106 34-35 0.05300928492018642 1106 36-37 0.0497231504975133 1106 38-39 0.16515870953644907 1106 40-41 0.22360003599683864 1106 42-43 0.1699861514602361 1106 44-45 0.26000955887139554 1106 46-47 0.22672626053121547 1106 48-49 0.2954298818868395 1106 50-51 0.27879692872463124 1106 52-53 0.3033844752525212 1106 54-55 0.3521622188824196 1106 56-57 0.36413265596285527 1106 58-59 0.3732910044349964 1106 60-61 0.32792910265558106 1106 62-63 0.32519906070524485 1106 64-65 0.371649340270487 1106 66-67 0.39119698873955855 1106 68-69 0.2984294966573202 1106 70-71 0.36545720843058405 1106 72-73 0.31850667610703454 1106 74-75 0.34843347225724486 1106 76-77 0.2385473817406627 1106 78-79 0.4802321332988697 1106 80-81 0.3052525399928889 1106 82-83 0.3769114333351631 1106 84-85 0.3317087198021511 1106 86-87 0.576618770095191 1106 88-89 0.6501908785577335 1106 90-91 0.7792684227079825 1106 92-93 0.6924277610141374 1106 94-95 0.8298531856877318 1106 96-97 0.8592912130688148 1106 98-99 0.799409715265277 1106 100-101 1.257938601822385 1107 1 0.050043237976147736 1107 2 0.029648854109275646 1107 3 0.040564216047293655 1107 4 0.04584602574797003 1107 5 0.030873461358417842 1107 6 0.020594800812453684 1107 7 0.0642524296687057 1107 8 0.09479410811852063 1107 9 -0.01581981913076902 1107 10-11 0.015950747450290237 1107 12-13 0.0065704769393803986 1107 14-15 0.038352882477767025 1107 16-17 0.06512273424984727 1107 18-19 0.050318518460137796 1107 20-21 0.10556371137155907 1107 22-23 0.08892768049062738 1107 24-25 0.14419652597641885 1107 26-27 0.09436202196509669 1107 28-29 0.09215996932341142 1107 30-31 0.08827928729753154 1107 32-33 0.14015007632963972 1107 34-35 0.12600381260599391 1107 36-37 0.18655449042623218 1107 38-39 0.014113330123201706 1107 40-41 0.016942487145016116 1107 42-43 0.08940205871531504 1107 44-45 0.10714029143337456 1107 46-47 0.04334425009367493 1107 48-49 -0.058600647352747615 1107 50-51 -0.06108798527372983 1107 52-53 -0.09642041001091428 1107 54-55 -0.13604938918481935 1107 56-57 -0.12172912381509349 1107 58-59 -0.12673589393143203 1107 60-61 -0.03270168435323484 1107 62-63 -0.02093399438033572 1107 64-65 -0.1825832924050914 1107 66-67 -0.1269160136544727 1107 68-69 -0.030681280729851323 1107 70-71 0.0500780877390028 1107 72-73 0.1357070031131613 1107 74-75 0.14392129160424005 1107 76-77 0.06422622012130219 1107 78-79 0.31568808092588085 1107 80-81 0.20258350692008875 1107 82-83 0.20456327908318883 1107 84-85 0.3311646576774905 1107 86-87 0.4018887695588482 1107 88-89 0.6631982296943697 1107 90-91 0.5383149690715037 1107 92-93 0.6236586335126297 1107 94-95 0.6153973859611064 1107 96-97 0.8023191727423935 1107 98-99 0.798416669833852 1107 100-101 1.0825515010112063 1108 1 0.05780672263064446 1108 2 0.016724555015592557 1108 3 0.056050355862268475 1108 4 0.04974784677700228 1108 5 0.06313144999336373 1108 6 0.029875529627325648 1108 7 0.038198507852989394 1108 8 0.09647954291062888 1108 9 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-0.9352262673774803 2316 54-55 -0.8221651538483314 2316 56-57 -0.9604578090266642 2316 58-59 -1.1046305732329031 2316 60-61 -0.8939469535393627 2316 62-63 -0.7968567643462166 2316 64-65 -1.0150571744538865 2316 66-67 -0.9997345336854693 2316 68-69 -0.7957346742145859 2316 70-71 -0.8228950981526459 2316 72-73 -0.7543337695087331 2316 74-75 -0.8396241579717945 2316 76-77 -0.8869688228539445 2316 78-79 -1.0293253116585213 2316 80-81 -1.0194380498352054 2316 82-83 -0.9603833595871336 2316 84-85 -1.2262805259761613 2316 86-87 -1.2147461707774276 2316 88-89 -1.5225866593545767 2316 90-91 -1.6092615037108438 2316 92-93 -1.6052859086474847 2316 94-95 -1.6154936630335648 2316 96-97 -1.4430320087954556 2316 98-99 -1.427793405634084 2316 100-101 -0.7884964003885031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 24.0 5 85.0 6 173.0 7 458.0 8 1041.0 9 1924.0 10 3311.0 11 5115.0 12 7681.0 13 10699.0 14 12990.0 15 17157.0 16 22492.0 17 32141.0 18 31750.0 19 21511.0 20 20300.0 21 22144.0 22 23851.0 23 26572.0 24 30597.0 25 36592.0 26 43682.0 27 52376.0 28 64023.0 29 78076.0 30 94241.0 31 115067.0 32 142416.0 33 180690.0 34 243813.0 35 349592.0 36 549461.0 37 818804.0 38 520852.0 39 26061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86138464526765 19.780920985624594 14.43284509967725 22.92484926943051 2 15.53417945227616 21.308249673551536 39.25619365108667 23.90137722308564 3 19.552620776230835 25.056468447103153 30.31459625857213 25.076314518093877 4 13.02337429218683 17.000931601181563 37.01979645581325 32.95589765081836 5 13.424841460105377 37.90652223383606 34.20331745370475 14.465318852353825 6 31.56381772991438 36.32681933173622 17.219681869699457 14.889681068649944 7 27.915328234888783 33.383059382715125 20.924743754239117 17.776868628156976 8 25.936181577136217 35.052679399750595 19.841054037026225 19.170084986086962 9 26.205295408489516 17.136721966801073 20.91972680053895 35.73825582417046 10-11 24.9110183667626 26.700213733314822 27.200481627555217 21.188286272367368 12-13 26.09985622685722 24.270788551477963 27.732153988880103 21.89720123278471 14-15 22.723441952012386 25.398937036405165 26.743794285818822 25.133826725763626 16-17 21.871814527121465 28.745485507124858 27.214642957474272 22.168057008279405 18-19 21.634694074688905 27.588291488479737 29.370273978127376 21.406740458703986 20-21 22.581364040416137 26.45709936367842 29.167030465104872 21.794506130800574 22-23 22.19971522551207 26.54648983706542 29.13839779564478 22.115397141777727 24-25 22.327024021121126 26.605451439777422 29.294930029346446 21.77259450975501 26-27 22.195283425354717 26.546479657150762 29.50314273387704 21.755094183617484 28-29 21.6736557543766 27.18055240263576 29.4521514277391 21.693640415248545 30-31 21.952858929082726 26.67086039647239 29.28386173497346 22.092418939471422 32-33 21.862927955158966 26.875072586450287 29.223284097892133 22.03871536049861 34-35 22.467614551482676 26.67244194459614 29.077156789093316 21.782786714827864 36-37 22.383667657444878 26.37956000797354 29.28229249868794 21.954479835893636 38-39 22.04160682421552 26.826993152110852 29.356731419871824 21.774668603801807 40-41 22.159291088517193 26.817884336749586 29.143321010134553 21.879503564598664 42-43 21.95108801407702 26.979747099303637 28.711862959733143 22.35730192688621 44-45 22.15367762579455 26.814975047869726 28.60684678471761 22.424500541618116 46-47 22.45242599796021 27.096756221343583 28.248618969063994 22.20219881163222 48-49 22.508488191372855 27.195710570206334 28.083571645768075 22.212229592652744 50-51 21.946660893904145 27.407548256571207 28.35123970721894 22.29455114230571 52-53 22.426656156010075 27.590736874083056 27.74527845063165 22.237328519275216 54-55 21.87943401001229 27.114791503996795 28.133399412988698 22.87237507300222 56-57 22.311695849537955 27.476109183325864 27.79985514585615 22.41233982128003 58-59 22.672574701849523 27.07886262376855 27.835000396369807 22.41356227801212 60-61 22.32564411624035 27.15530843696196 28.378700698184407 22.140346748613286 62-63 22.15620780308636 27.149536680226966 28.34338147971007 22.350874036976606 64-65 21.938094928574962 27.34161212295753 28.16574450413258 22.554548444334927 66-67 22.041682564501784 27.34111185012272 27.719680131630607 22.897525453744887 68-69 21.8185774385634 27.326808830876885 27.998278673358868 22.856335057200848 70-71 22.090013598442916 27.26234184811321 27.800195467222654 22.847449086221225 72-73 22.12691396090712 26.980996832666854 27.973244946250688 22.918844260175337 74-75 21.761244559307737 27.71577266491988 27.52874562021328 22.9942371555591 76-77 21.99160241693033 27.153914583465983 27.740433145942028 23.114049853661665 78-79 21.967433458373907 27.26007857238092 27.762721721108242 23.009766248136934 80-81 21.994159526608716 27.383452121409 27.669710433536864 22.952677918445417 82-83 22.010432216164766 27.22817565937263 27.795521134751933 22.96587098971067 84-85 21.84855874639181 27.179231889464994 27.598453002165886 23.373756361977314 86-87 21.859634218983093 27.122539498285008 27.752825229851208 23.26500105288069 88-89 21.887047793618166 27.048389395825772 27.482221326390388 23.582341484165678 90-91 21.767814376610385 26.90663719627617 27.68769251767729 23.63785590943615 92-93 21.972441679299134 27.13746480856441 27.495830523423493 23.394262988712963 94-95 21.791042583478152 26.824352351640947 27.536754112805976 23.84785095207493 96-97 21.51296078710183 26.793983646948377 27.761105415066993 23.931950150882802 98-99 21.556103817125663 26.956175384940323 27.273546212934484 24.21417458499953 100-101 21.66675490256678 26.12990689772831 28.132690902065633 24.070647297639276 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1220.0 1 869.0 2 618.0 3 737.0 4 676.0 5 694.0 6 1217.5 7 2371.0 8 3708.5 9 4460.0 10 4206.0 11 3843.5 12 4071.5 13 4454.0 14 4847.0 15 5229.5 16 5448.5 17 5692.0 18 6047.5 19 6377.5 20 7076.5 21 8205.5 22 9705.5 23 11864.0 24 14258.0 25 17093.0 26 21533.0 27 26687.5 28 30754.5 29 34966.0 30 40301.5 31 46705.5 32 52889.5 33 58489.5 34 65902.5 35 72685.0 36 80463.5 37 89913.0 38 94584.5 39 95915.5 40 98853.5 41 105022.0 42 111138.5 43 117614.5 44 125276.5 45 130500.0 46 131936.0 47 142740.0 48 173073.0 49 174384.5 50 148209.0 51 135726.0 52 128080.5 53 124261.5 54 120050.0 55 110343.0 56 99753.0 57 89047.5 58 78661.5 59 69109.0 60 58792.0 61 47187.5 62 37106.0 63 28805.5 64 21720.0 65 16433.0 66 11696.5 67 8737.5 68 6115.0 69 3670.5 70 2436.0 71 1575.0 72 1075.0 73 858.5 74 616.0 75 426.5 76 278.5 77 131.0 78 59.0 79 31.0 80 19.0 81 11.0 82 6.5 83 6.0 84 4.5 85 3.5 86 4.0 87 3.5 88 3.5 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39550780302608235 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.513724417347352E-4 16-17 0.002231297087644518 18-19 4.296286317824848E-4 20-21 0.0 22-23 0.0 24-25 1.2473089309814075E-4 26-27 1.385898812201564E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01211275561864167 36-37 0.023227664092498213 38-39 0.010477395020243824 40-41 0.005765339058758506 42-43 0.005127825605145787 44-45 0.0016353605983978456 46-47 0.0012611679191034232 48-49 0.0029935414343553782 50-51 5.543595248806257E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.92949406100782E-4 60-61 0.001455193752811642 62-63 0.0014829117290556734 64-65 9.285522041750479E-4 66-67 0.002647066731304987 68-69 0.002342168992620643 70-71 2.771797624403128E-5 72-73 0.0 74-75 0.0 76-77 0.09084566713981253 78-79 0.0038250807216763166 80-81 3.1875672680635973E-4 82-83 1.385898812201564E-5 84-85 1.385898812201564E-4 86-87 0.09340957994238541 88-89 0.438581538109307 90-91 0.3909204779576952 92-93 0.0 94-95 0.0011502960141272982 96-97 0.0 98-99 0.0 100-101 3.880516674164379E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3607767.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.88436285384696 #Duplication Level Percentage of deduplicated Percentage of total 1 70.11219974553687 31.469414138600737 2 15.146010443708319 13.596380570871197 3 5.830518513410269 7.8509732574593665 4 2.815916117989973 5.055624032234323 5 1.5926994074433591 3.574364906039739 6 0.9985767160196748 2.6892287795537984 7 0.6713170391322265 2.1092146302066723 8 0.4650023207887343 1.6697066315329994 9 0.3435238212053303 1.3876963055928084 >10 1.8031468847489036 14.569688161263592 >50 0.1329952147629564 4.171113948561172 >100 0.08075238695614083 6.702366062851067 >500 0.0044418483863097906 1.407465843471416 >1k 0.002529385879773731 2.1286297500456115 >5k 1.8507701559319984E-4 0.5265294017030335 >10k+ 1.8507701559319984E-4 1.0916035800124633 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13959 0.38691523039043263 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13776 0.3818428407377749 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11687 0.32393998836399357 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7260 0.2012325075316671 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6602 0.18299407916309451 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5153 0.14283073158549317 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4894 0.13565177573828907 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3850 0.10671420853952042 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.771797624403128E-5 0.0 3 0.0 0.0 0.0 2.771797624403128E-5 0.0 4 0.0 0.0 0.0 1.1087190497612511E-4 0.0 5 0.0 0.0 0.0 1.1087190497612511E-4 0.0 6 0.0 0.0 0.0 1.1087190497612511E-4 0.0 7 0.0 0.0 0.0 1.1087190497612511E-4 0.0 8 0.0 0.0 0.0 1.1087190497612511E-4 0.0 9 0.0 0.0 0.0 1.9402583370821896E-4 0.0 10-11 0.0 0.0 0.0 2.494617861962815E-4 0.0 12-13 0.0 0.0 0.0 3.3261571492837537E-4 0.0 14-15 0.0 0.0 0.0 3.6033369117240664E-4 0.0 16-17 0.0 0.0 0.0 5.682185130026412E-4 0.0 18-19 0.0 0.0 0.0 9.008342279310166E-4 0.0 20-21 0.0 0.0 0.0 9.978471447851262E-4 0.0 22-23 0.0 0.0 0.0 0.0012195909547373764 0.0 24-25 0.0 0.0 0.0 0.001579924645909783 0.0 26-27 0.0 0.0 0.0 0.0022590150638885495 0.0 28-29 0.0 0.0 0.0 0.00552973626068424 0.0 30-31 0.0 0.0 0.0 0.015037002112386968 0.0 32-33 0.0 0.0 0.0 0.03205583952622218 0.0 34-35 0.0 0.0 0.0 0.0589284174948105 0.0 36-37 0.0 0.0 0.0 0.10337419240211466 0.0 38-39 0.0 0.0 0.0 0.16664047317911607 0.0 40-41 0.0 0.0 0.0 0.23730745361327382 0.0 42-43 0.0 0.0 0.0 0.3111897192917392 0.0 44-45 0.0 0.0 0.0 0.4036430290537055 0.0 46-47 0.0 0.0 0.0 0.5163581794500587 0.0 48-49 0.0 0.0 0.0 0.6375827485533295 0.0 50-51 0.0 0.0 0.0 0.7694787385105524 0.0 52-53 0.0 0.0 0.0 0.9052413861538176 0.0 54-55 0.0 0.0 0.0 1.0561241898382017 0.0 56-57 0.0 0.0 0.0 1.2380927038802672 0.0 58-59 0.0 0.0 0.0 1.4389925956970058 0.0 60-61 0.0 0.0 0.0 1.6602513410649857 0.0 62-63 0.0 0.0 0.0 1.8850302694159573 0.0 64-65 0.0 0.0 0.0 2.122420876957963 0.0 66-67 0.0 0.0 0.0 2.399739229279496 0.0 68-69 0.0 0.0 0.0 2.7132988355401 0.0 70-71 0.0 0.0 0.0 3.0628502339535784 0.0 72-73 0.0 0.0 0.0 3.4174185860672264 0.0 74-75 0.0 0.0 0.0 3.792415086672726 0.0 76-77 0.0 0.0 0.0 4.1939099725675195 0.0 78-79 0.0 0.0 0.0 4.651755504166427 0.0 80-81 0.0 0.0 0.0 5.152550039955463 0.0 82-83 0.0 0.0 0.0 5.652166561754127 0.0 84-85 0.0 0.0 0.0 6.17900213622443 0.0 86-87 0.0 0.0 0.0 6.738240024924004 0.0 88-89 0.0 0.0 0.0 7.3341626551825545 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15360 0.0 24.163956 1 TACATGG 15595 0.0 23.096561 2 ACATGGG 15485 0.0 22.06542 3 GAGTACT 13235 0.0 21.46215 12-13 TTTGCGT 740 0.0 20.521503 3 AGTACTT 13705 0.0 20.08547 12-13 GTACTTT 14320 0.0 19.703432 14-15 CATGGGG 6440 0.0 18.64342 4 CATGGGA 10145 0.0 17.72875 4 AGAGTAC 25345 0.0 17.471188 10-11 CTTTGCG 870 0.0 17.455072 2 GTATCAA 36395 0.0 17.18877 1 GTATAGG 865 0.0 17.07526 1 CGATAGT 195 2.2931922E-4 17.035479 6 TTGCGTT 895 0.0 16.9675 4 CGCGGAA 395 0.0 16.917494 82-83 GTAAGCG 450 0.0 16.873587 94-95 AGTACAT 11805 0.0 16.602556 2 TACTTTT 16470 0.0 16.468563 14-15 ACTTTTT 17320 0.0 16.13888 16-17 >>END_MODULE