FastQCFastQC Report
Fri 27 May 2016
SRR939026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618892
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138880.3837638702674741No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT132980.367460537645224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108850.30078267049693663No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69720.19265565261411505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.18945025162397772No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55170.15244997640161684No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49590.13703089232837012No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38520.10644141908628386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG149450.024.7137451
TACATGG152100.024.0541342
ACATGGG155200.022.50633
GAGTACT120250.021.78572512-13
CTATCGT1404.9735355E-420.2841429
AGTACTT123950.020.10440812-13
GTACTTT132450.019.47542814-15
CATGGGA97250.019.1754724
CATGGGG67350.018.9742934
AGTACAT113250.018.9321272
GTATCAA345200.017.800461
AGAGTAC238700.017.10173410-11
ACTTTTT158900.016.44393316-17
ATGGGAG44850.016.251665
TACTTTT153950.016.079214-15
GGTATCA280750.016.0097891
CAACGCA382300.015.8840715
ATCAACG382600.015.8592453
TCAACGC384550.015.803444
TATCAAC385800.015.801312
Produced by FastQC (version 0.11.5)