##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3618892 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844308976338613 33.0 31.0 34.0 30.0 34.0 2 31.90876019510944 33.0 31.0 34.0 30.0 34.0 3 31.960653702846063 34.0 31.0 34.0 30.0 34.0 4 35.495252414274866 37.0 35.0 37.0 33.0 37.0 5 35.53288078229469 37.0 35.0 37.0 33.0 37.0 6 35.615335854178575 37.0 35.0 37.0 33.0 37.0 7 35.52858582129558 37.0 35.0 37.0 33.0 37.0 8 35.5137627760099 37.0 35.0 37.0 33.0 37.0 9 37.126285061836604 39.0 37.0 39.0 33.0 39.0 10-11 37.01325530024107 39.0 37.0 39.0 33.0 39.0 12-13 36.89330049086848 39.0 37.0 39.0 33.0 39.0 14-15 38.11513081904627 40.0 38.0 41.0 33.0 41.0 16-17 38.048485005907885 40.0 38.0 41.0 33.0 41.0 18-19 37.99396030608264 40.0 38.0 41.0 33.0 41.0 20-21 37.88060212904944 40.0 38.0 41.0 32.5 41.0 22-23 37.963038825143165 40.0 38.0 41.0 33.0 41.0 24-25 37.89836709689043 40.0 38.0 41.0 33.0 41.0 26-27 37.817506021179966 40.0 38.0 41.0 32.0 41.0 28-29 37.69562658957493 40.0 38.0 41.0 32.0 41.0 30-31 37.51375089392002 40.0 37.0 41.0 32.0 41.0 32-33 37.3319049034898 40.0 37.0 41.0 31.5 41.0 34-35 37.00992513730722 40.0 36.0 41.0 30.5 41.0 36-37 36.746995351063255 40.0 36.0 41.0 30.0 41.0 38-39 36.54671208756713 39.0 36.0 41.0 30.0 41.0 40-41 36.40359272948737 39.0 35.5 41.0 30.0 41.0 42-43 36.198537839758686 39.0 35.0 41.0 30.0 41.0 44-45 36.12347660554667 39.0 35.0 41.0 29.0 41.0 46-47 35.888801323720074 39.0 35.0 41.0 28.0 41.0 48-49 35.70433229286754 39.0 35.0 41.0 27.0 41.0 50-51 34.53866031370928 38.0 34.0 40.0 25.0 40.5 52-53 34.692155361364755 38.5 34.5 39.5 25.5 40.5 54-55 35.19089309656105 39.0 35.0 40.5 26.0 41.0 56-57 35.09064887816492 39.0 35.0 41.0 26.0 41.0 58-59 34.894009962165214 38.5 35.0 40.5 26.0 41.0 60-61 34.62517381010541 38.0 34.0 40.0 25.0 41.0 62-63 34.27513642297146 38.0 34.0 40.0 23.5 41.0 64-65 33.86998589623565 37.0 34.0 40.0 23.0 41.0 66-67 33.48174164910144 36.5 33.0 39.5 22.0 41.0 68-69 33.08427413694579 36.0 33.0 39.0 21.5 41.0 70-71 32.65488276522206 35.5 32.5 39.0 20.5 40.0 72-73 32.22129867373771 35.0 32.0 38.0 19.5 40.0 74-75 31.755225770760774 35.0 31.5 37.0 18.0 39.5 76-77 31.340712571693214 35.0 31.0 37.0 15.5 39.0 78-79 30.912288899475307 35.0 31.0 36.0 10.5 39.0 80-81 30.467915870382427 34.5 31.0 36.0 7.5 37.5 82-83 30.091038085690315 34.0 30.0 35.0 4.5 37.0 84-85 29.744262332227656 34.0 30.0 35.0 2.0 37.0 86-87 29.36360134538417 34.0 29.5 35.0 2.0 36.0 88-89 28.94899889247869 34.0 29.0 35.0 2.0 36.0 90-91 28.5216649184336 34.0 29.0 35.0 2.0 35.5 92-93 28.10379986470997 34.0 29.0 35.0 2.0 35.0 94-95 27.631228149389372 34.0 27.0 35.0 2.0 35.0 96-97 27.05209743755824 34.0 26.5 35.0 2.0 35.0 98-99 26.340366056793073 33.0 25.0 35.0 2.0 35.0 100-101 23.69423597056779 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35388078979335447 1101 2 0.39649816326477705 1101 3 0.3912871288489761 1101 4 0.26797529874720283 1101 5 0.1680068859861592 1101 6 0.2673794033481798 1101 7 0.19641437612454382 1101 8 0.31948541534637087 1101 9 0.30895784061038256 1101 10-11 0.42706113817022384 1101 12-13 0.5122436424794117 1101 14-15 0.364043580014652 1101 16-17 0.44319400805954956 1101 18-19 0.49695987988255297 1101 20-21 0.654027889782725 1101 22-23 0.5890330503386352 1101 24-25 0.5720611503128055 1101 26-27 0.5087356842294213 1101 28-29 0.47595175972470116 1101 30-31 0.5765585286547008 1101 32-33 0.5299959827467617 1101 34-35 0.46936524378282485 1101 36-37 0.49770736350346567 1101 38-39 0.4639199892089536 1101 40-41 0.4502495766209549 1101 42-43 0.43087159379170004 1101 44-45 0.4675867531890745 1101 46-47 0.6581657792725935 1101 48-49 0.6983249198652288 1101 50-51 0.600447551215062 1101 52-53 0.5525668759437181 1101 54-55 0.6333066598836865 1101 56-57 0.6021740774767252 1101 58-59 0.5493024835845546 1101 60-61 0.49665467958764964 1101 62-63 0.5255690870377734 1101 64-65 0.5177674569980937 1101 66-67 0.4888321817267851 1101 68-69 0.5263921011463424 1101 70-71 0.5441422490027321 1101 72-73 0.4777000354373797 1101 74-75 0.4704697429638287 1101 76-77 0.3807311220908005 1101 78-79 0.2396548550080304 1101 80-81 0.2591335678581359 1101 82-83 0.18165513581841353 1101 84-85 0.08536900308131834 1101 86-87 0.014909407015842646 1101 88-89 -0.005056433820726625 1101 90-91 0.004668083660806843 1101 92-93 0.17500205725685447 1101 94-95 0.1790631748628968 1101 96-97 0.02157932191448353 1101 98-99 0.05886621341634779 1101 100-101 0.4396691194122049 1102 1 0.24124964775044688 1102 2 0.13919699419890108 1102 3 0.08288787927325458 1102 4 0.1239953606496158 1102 5 0.12175495738129172 1102 6 0.1836536431518283 1102 7 0.20183396977943602 1102 8 0.08430362134104996 1102 9 0.2452621298558526 1102 10-11 0.1220486272753547 1102 12-13 0.2611492149143899 1102 14-15 0.33552727208657274 1102 16-17 0.21597876613842715 1102 18-19 0.2853375064954733 1102 20-21 0.41313836948143745 1102 22-23 0.3199217959332614 1102 24-25 0.340693771886599 1102 26-27 0.370521222485543 1102 28-29 0.2991734064757665 1102 30-31 0.31114980736362696 1102 32-33 0.2564855327657227 1102 34-35 0.10746274952739299 1102 36-37 0.18116504696114788 1102 38-39 0.21991695108827258 1102 40-41 0.2667330401632384 1102 42-43 0.14079698018767317 1102 44-45 0.23009186863536257 1102 46-47 0.27679172142794783 1102 48-49 0.4023809768624673 1102 50-51 0.19142374513243254 1102 52-53 0.21182415284805955 1102 54-55 0.1930448372689213 1102 56-57 0.07537284479314366 1102 58-59 -0.023308309614478162 1102 60-61 0.08362609008847954 1102 62-63 0.018338903337003387 1102 64-65 0.13243154225042275 1102 66-67 0.07704140228894829 1102 68-69 0.05099834634082612 1102 70-71 0.05179234488812767 1102 72-73 -0.02355930405725104 1102 74-75 0.0012788914200356771 1102 76-77 0.026776738724652915 1102 78-79 -0.030078414725235092 1102 80-81 -0.08546464438713741 1102 82-83 -0.2027492779977642 1102 84-85 -0.33915105379168153 1102 86-87 -0.25874879311294663 1102 88-89 -0.23076946835729117 1102 90-91 -0.19731113302429293 1102 92-93 -0.17688691227917275 1102 94-95 -0.21634317642580925 1102 96-97 -0.4413258615789353 1102 98-99 -0.3811244177507547 1102 100-101 0.010004145629583405 1103 1 0.08181792453873982 1103 2 0.22027877042403432 1103 3 0.3307914551464073 1103 4 0.29296550299600455 1103 5 0.3189803594048186 1103 6 0.28462896501655877 1103 7 0.2782997288080935 1103 8 0.20316908368222641 1103 9 0.2009707657868418 1103 10-11 0.3033600095814748 1103 12-13 0.4055681726744851 1103 14-15 0.4081485369255091 1103 16-17 0.4944656825715086 1103 18-19 0.4710114825439433 1103 20-21 0.5146479095515417 1103 22-23 0.49670570501677247 1103 24-25 0.4570154849022927 1103 26-27 0.36226382350258035 1103 28-29 0.34266878715663296 1103 30-31 0.3741534500922512 1103 32-33 0.36387438401062866 1103 34-35 0.31142600907870843 1103 36-37 0.3686860207606415 1103 38-39 0.31674973575019294 1103 40-41 0.3678009899731407 1103 42-43 0.2218535574408591 1103 44-45 0.20733094066670787 1103 46-47 0.10769897304906806 1103 48-49 0.04924671135254499 1103 50-51 -0.026680612673509074 1103 52-53 -0.014021459480879628 1103 54-55 0.10176747009921883 1103 56-57 0.2262328770165496 1103 58-59 0.06642123242635734 1103 60-61 0.16145937125703824 1103 62-63 0.1511134161583243 1103 64-65 7.536191740129539E-4 1103 66-67 0.01853468989107654 1103 68-69 0.1215237084109475 1103 70-71 0.06518068902543916 1103 72-73 0.1911318095513579 1103 74-75 0.15235650373228893 1103 76-77 0.036889911652338014 1103 78-79 0.15920435823219847 1103 80-81 -0.07297099409360186 1103 82-83 0.08830745044925337 1103 84-85 -0.008887766243432793 1103 86-87 0.01149445343223121 1103 88-89 -0.02034050017524791 1103 90-91 -0.06944811706882703 1103 92-93 -0.025298803552384896 1103 94-95 -0.03314443456703131 1103 96-97 -0.18622967362727394 1103 98-99 -0.11442801340075093 1103 100-101 0.1399434699808424 1104 1 0.04268163386056045 1104 2 0.1639571121719534 1104 3 0.0951967377807712 1104 4 0.16331749056306677 1104 5 0.16482415308666276 1104 6 0.18622282981655758 1104 7 0.14590482558929097 1104 8 0.12798360122842922 1104 9 0.1507720866329194 1104 10-11 0.0852413536489749 1104 12-13 0.13294250063135848 1104 14-15 0.1549071427335562 1104 16-17 0.03938998541332239 1104 18-19 0.1514371330087343 1104 20-21 0.22043453258884682 1104 22-23 0.17305904216693335 1104 24-25 0.22005546089000205 1104 26-27 0.17690864946720808 1104 28-29 0.0762279231166545 1104 30-31 0.20944870420606776 1104 32-33 0.1814818840655974 1104 34-35 0.15193973356161194 1104 36-37 0.11059269806080607 1104 38-39 0.07988230595604051 1104 40-41 0.09624085452394837 1104 42-43 0.12657738804789886 1104 44-45 0.2292431727677382 1104 46-47 0.3110080935411972 1104 48-49 0.3509033765937204 1104 50-51 0.27135597231195874 1104 52-53 0.3063361272027336 1104 54-55 0.2886447601336215 1104 56-57 0.2751821520886111 1104 58-59 0.2563528107107871 1104 60-61 0.15374571467940967 1104 62-63 0.11920441739797383 1104 64-65 0.09203379197792572 1104 66-67 0.24969144691684875 1104 68-69 0.2270615585240492 1104 70-71 0.23618346097163823 1104 72-73 0.2340084720074671 1104 74-75 0.07452013360972032 1104 76-77 0.09738845119479933 1104 78-79 0.07468002661927997 1104 80-81 0.01821713843825279 1104 82-83 0.01171417794544638 1104 84-85 0.11783183153375987 1104 86-87 0.11299280088323371 1104 88-89 0.11353437652913456 1104 90-91 0.24082027918226956 1104 92-93 0.22837206929789744 1104 94-95 0.1636762302466721 1104 96-97 0.060082835139990465 1104 98-99 0.16429980084561535 1104 100-101 0.7265343187574835 1105 1 0.06920691611634666 1105 2 0.09524398429810432 1105 3 0.1199808902595727 1105 4 0.10784154483632591 1105 5 0.10859187724885544 1105 6 0.10650191645413543 1105 7 0.15928269340295742 1105 8 0.17095480645988204 1105 9 0.1911296960412372 1105 10-11 0.09111175652576975 1105 12-13 0.14399161563420648 1105 14-15 0.16490152853234008 1105 16-17 0.24162542444683055 1105 18-19 0.14220961634715223 1105 20-21 0.22387682650252572 1105 22-23 0.23278452987999998 1105 24-25 0.2275077509253265 1105 26-27 0.11822528545635436 1105 28-29 0.23698778938095444 1105 30-31 0.21341323786201372 1105 32-33 0.18132111225510528 1105 34-35 0.11861033204274207 1105 36-37 0.20341655530800296 1105 38-39 0.2317405801082657 1105 40-41 0.20046859932444505 1105 42-43 0.15458515998039246 1105 44-45 0.06181102839084929 1105 46-47 0.3303679539201809 1105 48-49 0.33963182614510146 1105 50-51 0.2099996219659701 1105 52-53 0.21549017651975788 1105 54-55 0.2870734120012344 1105 56-57 0.14888787701986672 1105 58-59 0.12537358722293135 1105 60-61 0.3249954249595177 1105 62-63 0.28781184917203717 1105 64-65 0.31662899418339663 1105 66-67 0.29106555572853665 1105 68-69 0.20337222487815154 1105 70-71 -0.0023265620968402345 1105 72-73 0.12385469480395983 1105 74-75 0.14513933735199203 1105 76-77 0.034029479235055504 1105 78-79 -0.03994878640037669 1105 80-81 -0.07980642632823631 1105 82-83 0.015291206075634278 1105 84-85 0.04373251737225203 1105 86-87 0.0949799933627169 1105 88-89 0.11804958463315884 1105 90-91 0.12041281480748722 1105 92-93 0.26609021060714255 1105 94-95 0.24357986139166599 1105 96-97 0.19479812272893327 1105 98-99 0.24051890579381663 1105 100-101 0.527140531806829 1106 1 0.23534296664692178 1106 2 0.20519420192839632 1106 3 0.2452657759031176 1106 4 0.1452852539867564 1106 5 0.20576102367082427 1106 6 0.16814414732737504 1106 7 0.20346630212042527 1106 8 0.1884817373301857 1106 9 0.26732200230942027 1106 10-11 0.248868338740742 1106 12-13 0.25404956312970484 1106 14-15 0.29662578731894484 1106 16-17 0.3973822211639302 1106 18-19 0.3350698554404232 1106 20-21 0.33604340655970333 1106 22-23 0.2751852492282154 1106 24-25 0.31948719064065045 1106 26-27 0.24019304885029413 1106 28-29 0.3433826022597586 1106 30-31 0.38479214183273314 1106 32-33 0.33586207769344867 1106 34-35 0.3076997066541125 1106 36-37 0.2722809963448114 1106 38-39 0.3757412555346207 1106 40-41 0.34647177344528046 1106 42-43 0.40586243718226456 1106 44-45 0.44421709752437266 1106 46-47 0.49958140780031357 1106 48-49 0.5554062873680934 1106 50-51 0.5015163180202791 1106 52-53 0.37150887650915365 1106 54-55 0.2510806246354136 1106 56-57 0.388717220145395 1106 58-59 0.5781824798684383 1106 60-61 0.5876896738764685 1106 62-63 0.5213887105206609 1106 64-65 0.40164126421604607 1106 66-67 0.5356782644691194 1106 68-69 0.47193575645146524 1106 70-71 0.4511995720512516 1106 72-73 0.2923408523940836 1106 74-75 0.31440577836581696 1106 76-77 0.35676102343335003 1106 78-79 0.3905365629763544 1106 80-81 0.35409579517330414 1106 82-83 0.4170219382301461 1106 84-85 0.4982722698521158 1106 86-87 0.5413828692234404 1106 88-89 0.6708716921421392 1106 90-91 0.45498746218768815 1106 92-93 0.7053928105517322 1106 94-95 0.7408446400139752 1106 96-97 0.928122337593674 1106 98-99 0.9380263718724748 1106 100-101 1.2534841706056703 1107 1 0.028177486633595095 1107 2 0.052009404497344036 1107 3 0.06715905879583417 1107 4 0.09730026049083307 1107 5 0.0022884130544085224 1107 6 0.028387280343565635 1107 7 -0.056093117477260535 1107 8 -0.020296564823382823 1107 9 0.0664494507638409 1107 10-11 0.026892262426251534 1107 12-13 0.04406580760751666 1107 14-15 0.04600350127788744 1107 16-17 0.06925099594696604 1107 18-19 0.1483672330726904 1107 20-21 0.14800744876392713 1107 22-23 0.04032214901518927 1107 24-25 0.04469016806176995 1107 26-27 0.13771003171346052 1107 28-29 0.08013581187950791 1107 30-31 0.16173472373066744 1107 32-33 0.15885838262460794 1107 34-35 0.022438035452395866 1107 36-37 0.13953203479740495 1107 38-39 0.11323253630329333 1107 40-41 0.03750149109928458 1107 42-43 0.05118390188744826 1107 44-45 0.19926576811652552 1107 46-47 0.1205956979134939 1107 48-49 0.08649341055590298 1107 50-51 0.04620308552629382 1107 52-53 0.0035221234566407134 1107 54-55 0.11155438524774297 1107 56-57 0.09943480502828805 1107 58-59 0.06289586066790775 1107 60-61 0.052159198984711 1107 62-63 0.015060119611291611 1107 64-65 0.14216149597478278 1107 66-67 -0.014074140781907829 1107 68-69 0.022128050850419356 1107 70-71 0.042378223602497656 1107 72-73 0.02674976706186527 1107 74-75 0.06646477378468774 1107 76-77 0.11855696514174952 1107 78-79 0.19647867984861733 1107 80-81 0.11874064752765179 1107 82-83 0.17565303462242454 1107 84-85 0.12438220443431192 1107 86-87 0.13763784154184577 1107 88-89 0.23046913073047293 1107 90-91 0.2269583540943536 1107 92-93 0.20073548419797405 1107 94-95 0.33568285506949636 1107 96-97 0.3136812481767812 1107 98-99 0.36837196763948654 1107 100-101 0.5171996000317414 1108 1 0.09658822385343058 1108 2 0.06973687568002518 1108 3 0.1108426722023772 1108 4 0.08307420906103857 1108 5 0.14084370693210246 1108 6 0.13484978516371626 1108 7 0.1099021277049772 1108 8 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-1.8449599497560278 2316 80-81 -1.8441822948958304 2316 82-83 -1.9422440846567497 2316 84-85 -2.0800849215912223 2316 86-87 -2.049532199567995 2316 88-89 -2.1602874090692588 2316 90-91 -2.4552160961531335 2316 92-93 -2.611413458746018 2316 94-95 -2.8237281270943413 2316 96-97 -2.948219633398079 2316 98-99 -3.4572053893541153 2316 100-101 -3.9261510666450334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10976.0 3 2745.0 4 2699.0 5 3014.0 6 3698.0 7 4407.0 8 5577.0 9 6786.0 10 8594.0 11 10911.0 12 14750.0 13 21837.0 14 21329.0 15 19706.0 16 24626.0 17 32864.0 18 27987.0 19 20394.0 20 20385.0 21 21731.0 22 23916.0 23 26609.0 24 30237.0 25 34996.0 26 40637.0 27 47861.0 28 56290.0 29 67554.0 30 81470.0 31 98711.0 32 123824.0 33 158682.0 34 214416.0 35 305126.0 36 471410.0 37 743264.0 38 694776.0 39 114055.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99618663854682 19.100562165967137 13.90330523658222 22.999945958903826 2 15.195652047651354 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22.699965699617163 26.450657239593706 29.054941468056384 21.794435592732743 22-23 22.328824216080193 26.472197296576876 29.06798866110991 22.130989826233016 24-25 22.467226314129345 26.51640206077174 29.159256041481058 21.857115583617862 26-27 22.21582126270548 26.480091609352023 29.462030250621083 21.842056877321415 28-29 21.71013323907987 27.127927638791572 29.374489450782193 21.78744967134637 30-31 21.986663708333428 26.653134689740188 29.205679225197034 22.154522376729354 32-33 21.884769146437762 26.82720600388978 29.161268227383303 22.126756622289157 34-35 22.520620692984 26.64148594375282 28.981484512357437 21.856408850905744 36-37 22.45235786374094 26.341034177415228 29.17580226604098 22.030805692802854 38-39 22.106174472768508 26.794379717846383 29.266489533190292 21.832956276194817 40-41 22.16875520049786 26.834346692448634 29.10277086693254 21.894127240120966 42-43 21.975209832696592 26.922794212153867 28.64601722569946 22.45597872945008 44-45 22.17649614426827 26.784139340582737 28.560016299614944 22.479348215534056 46-47 22.4473931238809 27.037318312160885 28.269630693997343 22.245657869960876 48-49 22.49149030480617 27.10170255062207 28.103823493722444 22.302983650849313 50-51 21.96936540844848 27.339084586958094 28.398282212442993 22.293267792150427 52-53 22.444514340730308 27.524831520377184 27.813165282029896 22.217488856862612 54-55 21.947281108450056 27.030401104236347 28.18439034843843 22.837927438875163 56-57 22.34234401957763 27.397208125706314 27.845037833433967 22.41541002128209 58-59 22.67404430640545 27.004712164519812 27.87879242552688 22.442451103547864 60-61 22.40168823077486 26.981283582475022 28.499808174528273 22.117220012221843 62-63 22.22479450423456 27.03286667721827 28.45370663599197 22.288632182555194 64-65 21.972785673556526 27.241099282193225 28.29793137423619 22.48818367001406 66-67 22.080962737599453 27.20011488966755 27.844047564099927 22.874874808633074 68-69 21.844232312972363 27.179237500093844 28.185297159081273 22.79123302785252 70-71 22.115348122053163 27.11208457555475 27.99782291211224 22.774744390279842 72-73 22.112812502910554 26.77960696741975 28.237558859177668 22.87002167049203 74-75 21.73395332389104 27.538920967884756 27.853813933051086 22.87331177517312 76-77 22.038560180146348 26.92979148549438 27.944212672092533 23.087435662266735 78-79 21.99438468441154 27.09787173258581 27.995041092182493 22.912702490820156 80-81 22.000820051495676 27.175995250315093 27.97350991905222 22.84967477913701 82-83 22.033984509947192 26.983219526349128 28.12335066957083 22.85944529413285 84-85 21.86795334932236 26.933182272831445 27.971978017472672 23.226886360373523 86-87 21.8825605714246 26.859791135414568 28.194314936666643 23.063333356494187 88-89 21.9235221511075 26.752973515459054 27.93693154913835 23.386572784295094 90-91 21.82659472479063 26.607095968789046 28.238645864739098 23.327663441681224 92-93 22.06573677127445 26.747418423316716 28.121965605668066 23.06487919974077 94-95 21.865390753326018 26.476730957218592 28.095977642323888 23.561900647131502 96-97 21.604828438847587 26.368948708888308 28.380881632966254 23.64534121929785 98-99 21.6818939249766 26.4935527830483 27.942583160041977 23.88197013193312 100-101 21.74999218226103 25.86720962311549 28.595426796429553 23.78737139819393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4259.0 1 2511.0 2 868.5 3 964.0 4 866.0 5 885.5 6 1493.5 7 2823.0 8 4407.0 9 5259.0 10 4941.5 11 4582.0 12 4981.0 13 5486.0 14 5840.5 15 6325.5 16 6800.5 17 6939.5 18 7109.5 19 7485.5 20 7937.0 21 8922.0 22 10282.5 23 11920.5 24 14134.0 25 17174.5 26 21557.0 27 26628.0 28 30470.5 29 34695.0 30 40256.0 31 46347.5 32 52250.5 33 58363.0 34 66324.0 35 72332.5 36 80260.0 37 89984.5 38 94755.5 39 96395.0 40 98975.0 41 105387.0 42 111409.0 43 117764.5 44 125205.5 45 130097.0 46 132590.0 47 143314.5 48 172808.0 49 174747.0 50 147779.5 51 134065.5 52 126415.5 53 122577.5 54 118030.5 55 108847.0 56 98998.5 57 88162.5 58 77638.5 59 68259.0 60 58153.0 61 47009.5 62 37252.0 63 29114.5 64 22261.5 65 16954.0 66 12128.5 67 9151.0 68 6631.0 69 4154.5 70 2811.0 71 1908.0 72 1355.5 73 998.0 74 709.0 75 505.5 76 344.0 77 184.5 78 115.5 79 78.5 80 50.0 81 45.5 82 37.5 83 27.5 84 27.5 85 26.0 86 18.5 87 14.0 88 10.5 89 7.5 90 8.5 91 7.0 92 4.5 93 8.0 94 8.5 95 4.0 96 2.0 97 1.0 98 1.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29089013985496115 2 0.08878408087337229 3 0.09276319934388758 4 0.10760199530685083 5 0.10961918730926482 6 0.08571684371901676 7 0.09480802411345793 8 0.09163025589047698 9 0.09624492800558845 10-11 0.09011045369687738 12-13 0.09399285748234543 14-15 0.10261428083512855 16-17 0.09150590843827337 18-19 0.10188201250548509 20-21 0.10423079771377539 22-23 0.11750834233240451 24-25 0.13020559884075017 26-27 0.13403273709190547 28-29 0.1250244549989334 30-31 0.11727346381157548 32-33 0.11151203185947522 34-35 0.38735612999780045 36-37 0.5933445927648573 38-39 0.5761984607443383 40-41 0.5382034059043486 42-43 0.5661815826501593 44-45 0.587500262511288 46-47 0.576405706498011 48-49 0.5988435134289722 50-51 0.5901668245418763 52-53 0.5940630447109226 54-55 0.5833691638214128 56-57 0.5551699249383513 58-59 0.5501407613158944 60-61 0.4963535800460472 62-63 0.5720949948216194 64-65 0.60689846505505 66-67 0.6189187187680649 68-69 0.6213227695106679 70-71 0.6174403657251999 72-73 0.6097308236885766 74-75 0.6066912193013774 76-77 0.5905813160492217 78-79 0.5669967492812718 80-81 0.5619814020423931 82-83 0.5758945003056184 84-85 0.5862706043728301 86-87 0.5766129522516836 88-89 0.5896970675002183 90-91 0.6029884285024256 92-93 0.6108913999091434 94-95 0.6105736230868454 96-97 0.6066221373834865 98-99 0.6108913999091434 100-101 0.5887022878825895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3618892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.52646962900232 #Duplication Level Percentage of deduplicated Percentage of total 1 71.74942905626624 33.38247631884628 2 14.335910396575438 13.339985993407314 3 5.506799707044025 7.686358479683481 4 2.6376770173467174 4.908871985547979 5 1.558412149466158 3.6253707770802714 6 0.9264175325110406 2.586176231409012 7 0.6255061973654407 2.0371816566133134 8 0.4417504267114426 1.6442470249590997 9 0.32785310311365756 1.372846270031258 >10 1.6820258201624707 13.95963986981987 >50 0.12763166375792812 4.147454141753328 >100 0.07391403915716602 6.375282023432556 >500 0.003932239062153632 1.3119407734706394 >1k 0.002383175182750897 2.0305864876664166 >5k 1.7873813870631727E-4 0.5362469196334189 >10k+ 1.7873813870631727E-4 1.0553350466458458 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13888 0.3837638702674741 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13298 0.367460537645224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10885 0.30078267049693663 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6972 0.19265565261411505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6856 0.18945025162397772 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5517 0.15244997640161684 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4959 0.13703089232837012 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3852 0.10644141908628386 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.7632767156356143E-5 0.0 3 0.0 0.0 0.0 8.289830146906844E-5 0.0 4 0.0 0.0 0.0 1.381638357817807E-4 0.0 5 0.0 0.0 0.0 1.381638357817807E-4 0.0 6 0.0 0.0 0.0 1.381638357817807E-4 0.0 7 0.0 0.0 0.0 1.381638357817807E-4 0.0 8 0.0 0.0 0.0 1.381638357817807E-4 0.0 9 0.0 0.0 0.0 1.6579660293813687E-4 0.0 10-11 0.0 0.0 0.0 2.486949044072053E-4 0.0 12-13 0.0 0.0 0.0 3.3159320587627375E-4 0.0 14-15 0.0 0.0 0.0 3.3159320587627375E-4 0.0 16-17 0.0 0.0 0.0 4.421242745016983E-4 0.0 18-19 0.0 0.0 0.0 5.941044938616571E-4 0.0 20-21 0.0 0.0 0.0 6.908191789089036E-4 0.0 22-23 0.0 0.0 0.0 0.0010085960012069992 0.0 24-25 0.0 0.0 0.0 0.0013401892070832731 0.0 26-27 0.0 0.0 0.0 0.0018099462487413277 0.0 28-29 0.0 2.7632767156356143E-5 0.0 0.005346940444754914 0.0 30-31 0.0 2.7632767156356143E-5 0.0 0.01564014621049758 0.0 32-33 0.0 2.7632767156356143E-5 0.0 0.034430427876819755 0.0 34-35 0.0 2.7632767156356143E-5 0.0 0.06242242100620853 0.0 36-37 0.0 2.7632767156356143E-5 0.0 0.10709078911445824 0.0 38-39 0.0 2.7632767156356143E-5 0.0 0.17539898952497063 0.0 40-41 0.0 2.7632767156356143E-5 0.0 0.24770012478957648 0.0 42-43 0.0 2.7632767156356143E-5 0.0 0.32573505923912627 0.0 44-45 0.0 2.7632767156356143E-5 0.0 0.4193825071320172 0.0 46-47 0.0 2.7632767156356143E-5 0.0 0.5339341433786916 0.0 48-49 0.0 2.7632767156356143E-5 0.0 0.6580881662121998 0.0 50-51 0.0 2.7632767156356143E-5 0.0 0.7944420557452392 0.0 52-53 0.0 2.7632767156356143E-5 0.0 0.9306992305932313 0.0 54-55 0.0 2.7632767156356143E-5 0.0 1.0841439866124771 0.0 56-57 0.0 2.7632767156356143E-5 0.0 1.2685236254632635 0.0 58-59 0.0 2.7632767156356143E-5 0.0 1.4730199188038768 0.0 60-61 0.0 2.7632767156356143E-5 0.0 1.6997467733217793 0.0 62-63 0.0 2.7632767156356143E-5 0.0 1.9300658875700076 0.0 64-65 0.0 2.7632767156356143E-5 0.0 2.169600529664881 0.0 66-67 0.0 2.7632767156356143E-5 0.0 2.4468953480789146 0.0 68-69 0.0 2.7632767156356143E-5 0.0 2.765625500843905 0.0 70-71 0.0 2.7632767156356143E-5 0.0 3.1185788357320416 0.0 72-73 0.0 2.7632767156356143E-5 0.0 3.4721124587304626 0.0 74-75 0.0 2.7632767156356143E-5 0.0 3.84902340274316 0.0 76-77 0.0 2.7632767156356143E-5 0.0 4.251715718512738 0.0 78-79 0.0 2.7632767156356143E-5 0.0 4.711607862295974 0.0 80-81 0.0 2.7632767156356143E-5 0.0 5.21380577259559 0.0 82-83 0.0 2.7632767156356143E-5 0.0 5.721654583778681 0.0 84-85 0.0 2.7632767156356143E-5 0.0 6.247630490216343 0.0 86-87 0.0 2.7632767156356143E-5 0.0 6.809902036313877 0.0 88-89 0.0 2.7632767156356143E-5 0.0 7.416620888382411 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14945 0.0 24.713745 1 TACATGG 15210 0.0 24.054134 2 ACATGGG 15520 0.0 22.5063 3 GAGTACT 12025 0.0 21.785725 12-13 CTATCGT 140 4.9735355E-4 20.284142 9 AGTACTT 12395 0.0 20.104408 12-13 GTACTTT 13245 0.0 19.475428 14-15 CATGGGA 9725 0.0 19.175472 4 CATGGGG 6735 0.0 18.974293 4 AGTACAT 11325 0.0 18.932127 2 GTATCAA 34520 0.0 17.80046 1 AGAGTAC 23870 0.0 17.101734 10-11 ACTTTTT 15890 0.0 16.443933 16-17 ATGGGAG 4485 0.0 16.25166 5 TACTTTT 15395 0.0 16.0792 14-15 GGTATCA 28075 0.0 16.009789 1 CAACGCA 38230 0.0 15.884071 5 ATCAACG 38260 0.0 15.859245 3 TCAACGC 38455 0.0 15.80344 4 TATCAAC 38580 0.0 15.80131 2 >>END_MODULE