FastQCFastQC Report
Fri 27 May 2016
SRR939026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618892
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143530.3966131069951797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139790.38627845207870254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116500.32192173737154905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74670.20633387235651132No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66610.18406186202848826No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52330.1446022705292117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47360.1308687852525027No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38810.1072427693338182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG153150.025.2940861
TACATGG154200.024.3721872
ACATGGG152500.023.648163
GAGTACT128000.021.15121712-13
CATGGGA100700.019.6970184
GTACTTT138250.019.37708514-15
CATGGGG62750.019.0563834
AGTACTT133450.018.93610812-13
GTGTAGC18850.017.9470251
TCGCGCA2704.9326627E-717.574789
AGTACAT113550.017.4679832
GCACCGT7200.017.135416
GGACCGT3601.9372237E-917.135416
GTACCGT2807.2187686E-716.947116
TTTGCGT7300.016.9006793
GTTCGCG2606.694916E-616.425667
AGAGTAC243900.016.36318610-11
ACTTTTT166750.016.27825416-17
GTATCAA351300.016.2760311
ATGGGAG44350.015.9421295
Produced by FastQC (version 0.11.5)