##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3618892 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.166252543596215 33.0 31.0 34.0 30.0 34.0 2 32.39027111060513 34.0 31.0 34.0 31.0 34.0 3 32.487025034181734 34.0 31.0 34.0 30.0 34.0 4 36.058321718360204 37.0 35.0 37.0 35.0 37.0 5 35.974670700313794 37.0 35.0 37.0 35.0 37.0 6 36.01789249306141 37.0 35.0 37.0 35.0 37.0 7 35.95940332013224 37.0 35.0 37.0 35.0 37.0 8 35.92700445329675 37.0 35.0 37.0 35.0 37.0 9 37.60429601104426 39.0 37.0 39.0 35.0 39.0 10-11 37.52619641591957 39.0 37.0 39.0 35.0 39.0 12-13 37.45534130336026 39.0 37.0 39.0 34.0 39.0 14-15 38.70919994296597 40.0 38.0 41.0 34.0 41.0 16-17 38.703920702800744 40.0 38.0 41.0 34.0 41.0 18-19 38.80833512025228 40.0 38.0 41.0 34.0 41.0 20-21 38.81049572631623 40.0 38.0 41.0 34.0 41.0 22-23 38.75345934612031 40.0 38.0 41.0 34.0 41.0 24-25 38.68847909802227 40.0 38.0 41.0 34.0 41.0 26-27 38.536443336800325 40.0 38.0 41.0 34.0 41.0 28-29 38.35630270259516 40.0 38.0 41.0 34.0 41.0 30-31 38.19645916484935 40.0 38.0 41.0 33.5 41.0 32-33 38.04046031216184 40.0 37.0 41.0 33.0 41.0 34-35 38.00761835390501 40.0 37.0 41.0 33.0 41.0 36-37 37.90461293125078 40.0 37.0 41.0 33.0 41.0 38-39 37.78353706051466 40.0 37.0 41.0 33.0 41.0 40-41 37.63653820008997 40.0 37.0 41.0 33.0 41.0 42-43 37.46543237543425 40.0 37.0 41.0 32.0 41.0 44-45 37.215957674337886 40.0 37.0 41.0 31.5 41.0 46-47 36.907253794807914 40.0 36.0 41.0 31.0 41.0 48-49 36.61101063529942 40.0 36.0 41.0 30.0 41.0 50-51 36.34508739138941 39.0 36.0 41.0 30.0 41.0 52-53 36.126365887680535 39.0 35.0 41.0 29.5 41.0 54-55 35.87874493076886 39.0 35.0 40.0 29.0 41.0 56-57 35.60907192035573 38.5 35.0 40.0 28.0 41.0 58-59 35.304402010339075 38.0 34.5 40.0 28.0 41.0 60-61 34.89421748424655 38.0 34.0 40.0 27.0 41.0 62-63 34.59326625386997 37.0 34.0 40.0 26.0 41.0 64-65 34.33734690065357 37.0 34.0 40.0 26.0 41.0 66-67 33.8844574527231 36.5 33.0 39.0 26.0 41.0 68-69 33.464129628626665 36.0 33.0 39.0 25.0 40.0 70-71 33.00343958869179 35.0 32.5 38.5 24.5 40.0 72-73 32.48989469705092 35.0 32.0 37.5 23.0 39.5 74-75 32.02692177605742 35.0 31.5 37.0 22.0 39.0 76-77 30.050431596190215 33.0 29.5 35.0 19.5 37.5 78-79 31.027373709964266 34.0 31.0 36.0 20.0 38.0 80-81 31.025090967069477 34.0 31.0 36.0 20.0 37.0 82-83 30.70986451101608 34.0 31.0 35.0 18.5 37.0 84-85 30.316284100216308 34.0 30.5 35.0 17.5 36.5 86-87 29.75431831068736 34.0 30.0 35.0 9.0 36.0 88-89 29.19196041219246 34.0 29.0 35.0 4.5 35.5 90-91 28.74070088303271 34.0 29.0 35.0 2.0 35.0 92-93 28.39007823389037 34.0 29.0 35.0 2.0 35.0 94-95 28.002654818104546 33.0 28.5 35.0 2.0 35.0 96-97 27.393414476032994 33.0 27.0 35.0 2.0 35.0 98-99 26.51338337811684 33.0 25.5 34.5 2.0 35.0 100-101 22.233562510293204 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.219396865461853 1101 2 0.22064997234657113 1101 3 0.2255566767442403 1101 4 0.09181286880154005 1101 5 0.21661546985073699 1101 6 0.14672952360811564 1101 7 0.149817771510385 1101 8 0.21318182952865072 1101 9 0.26000162612672284 1101 10-11 0.29824524714099 1101 12-13 0.29807872281629244 1101 14-15 0.35319203434236357 1101 16-17 0.31325349246970546 1101 18-19 0.3587157684460962 1101 20-21 0.3926741114417567 1101 22-23 0.39725326687776885 1101 24-25 0.36940666368834485 1101 26-27 0.31875181146717324 1101 28-29 0.36999797607757756 1101 30-31 0.3111300645225512 1101 32-33 0.3704199118753593 1101 34-35 0.49957241289902043 1101 36-37 0.5004177312510762 1101 38-39 0.5366870252063123 1101 40-41 0.5435200416638608 1101 42-43 0.3744672296866156 1101 44-45 0.47845929851511215 1101 46-47 0.3551999767051015 1101 48-49 0.1688363034160787 1101 50-51 0.33933333033819935 1101 52-53 0.3540307144926871 1101 54-55 0.3493294443618211 1101 56-57 0.2662060564649238 1101 58-59 0.2729965949649653 1101 60-61 0.3943444788519699 1101 62-63 0.2573996989693015 1101 64-65 0.2325831480000744 1101 66-67 0.2928454040139741 1101 68-69 0.2674598075757544 1101 70-71 0.25245625851266595 1101 72-73 0.13933166064496305 1101 74-75 0.1502378403943112 1101 76-77 0.015928589143175742 1101 78-79 0.19810532745033882 1101 80-81 0.2717510028933212 1101 82-83 0.34280400981955594 1101 84-85 0.3301490749720912 1101 86-87 0.36992699791122163 1101 88-89 0.5845839155100734 1101 90-91 0.532240097519793 1101 92-93 0.5753139514060415 1101 94-95 0.5656248478293087 1101 96-97 0.41287519095785186 1101 98-99 0.52674543756331 1101 100-101 1.0387058841455499 1102 1 0.08592408493024806 1102 2 0.05444027672510998 1102 3 0.12911143324017615 1102 4 0.11522798045194094 1102 5 0.08706272265093418 1102 6 0.09113717389811882 1102 7 0.058007709112089856 1102 8 0.15670412599212113 1102 9 0.14212718472286667 1102 10-11 0.1889946673021754 1102 12-13 0.19479016364537927 1102 14-15 0.2514438884765724 1102 16-17 0.27418520340141583 1102 18-19 0.1405128884785043 1102 20-21 0.21262482845130393 1102 22-23 0.2138044510760011 1102 24-25 0.2357325300142108 1102 26-27 0.19286003851657796 1102 28-29 0.18667463098952908 1102 30-31 0.12029735898396865 1102 32-33 0.13239071796381552 1102 34-35 0.23107082086801967 1102 36-37 0.22407770491104628 1102 38-39 0.2473045740591573 1102 40-41 0.171760287551173 1102 42-43 0.14759645869819593 1102 44-45 0.18050063290937857 1102 46-47 0.3294231126929432 1102 48-49 0.2712117587327114 1102 50-51 0.26264169482303146 1102 52-53 0.16186733115284113 1102 54-55 0.2758938709262537 1102 56-57 0.30202216581044183 1102 58-59 0.1605524236972684 1102 60-61 0.06190779347409858 1102 62-63 0.08576793910224012 1102 64-65 0.1217721254243429 1102 66-67 0.107458762744983 1102 68-69 0.24273457696817502 1102 70-71 0.18762725250719825 1102 72-73 0.24531664898290018 1102 74-75 0.17616849867791018 1102 76-77 -0.10675988648647916 1102 78-79 -0.02858231100200115 1102 80-81 0.11587565584150283 1102 82-83 0.16843893839565993 1102 84-85 0.0535870925277564 1102 86-87 0.19746847251152033 1102 88-89 0.48826012800981644 1102 90-91 0.4019112318968112 1102 92-93 0.4330477650222093 1102 94-95 0.4649064088755779 1102 96-97 0.3673219542281423 1102 98-99 0.4206835520896597 1102 100-101 0.9832157110083202 1103 1 0.0772235750594632 1103 2 0.07007702085805079 1103 3 0.08775123096715731 1103 4 0.07911872257557206 1103 5 0.07044426132796389 1103 6 0.05915463869732207 1103 7 0.08999537100027766 1103 8 0.08054805363943984 1103 9 0.12075975990313736 1103 10-11 0.1654106887737825 1103 12-13 0.10112901717557321 1103 14-15 0.23295436003970593 1103 16-17 0.042266856064692604 1103 18-19 0.1255873201591342 1103 20-21 0.16629942943426812 1103 22-23 0.09314195446266638 1103 24-25 0.19453772857949758 1103 26-27 0.10747762326288779 1103 28-29 0.18422133728404333 1103 30-31 0.21801905910473351 1103 32-33 0.2559952814455002 1103 34-35 0.1381526530522521 1103 36-37 -0.05289331957313692 1103 38-39 0.07918641764374712 1103 40-41 -0.043719510433014364 1103 42-43 0.14876738541037327 1103 44-45 0.16664098110356207 1103 46-47 0.19132740247903968 1103 48-49 0.14478838611272948 1103 50-51 -0.02593241352872866 1103 52-53 0.04252287111206243 1103 54-55 0.0981901993665204 1103 56-57 0.10787594781987053 1103 58-59 -0.031911328450512144 1103 60-61 0.04945137683395728 1103 62-63 0.11317060459736439 1103 64-65 0.11373087279561389 1103 66-67 0.14444438004071714 1103 68-69 0.1230793875353342 1103 70-71 0.09694962086993542 1103 72-73 0.11512911475791299 1103 74-75 0.04463321047205682 1103 76-77 -0.04808225554436518 1103 78-79 0.06853291937829553 1103 80-81 0.2566020083876346 1103 82-83 0.2970070042077282 1103 84-85 0.15312211935944475 1103 86-87 0.021118202978101408 1103 88-89 0.25595488956063406 1103 90-91 0.42735446009018574 1103 92-93 0.29078119048530127 1103 94-95 0.2933348636937332 1103 96-97 0.008649649827344064 1103 98-99 0.222257679731662 1103 100-101 0.6952472903599691 1104 1 -0.0820861194912581 1104 2 -0.02260054485401497 1104 3 0.010005835835713128 1104 4 0.00885091776844149 1104 5 0.008437582272222244 1104 6 -0.0044567795461105675 1104 7 -0.019521047705922 1104 8 0.09586500369071871 1104 9 0.06303764073382467 1104 10-11 0.0010623006631433896 1104 12-13 0.07662146245890966 1104 14-15 0.08105032804513712 1104 16-17 0.03670928547108332 1104 18-19 0.06300662512895627 1104 20-21 0.0235690428327473 1104 22-23 0.03181927098421511 1104 24-25 0.0733349650532844 1104 26-27 0.07529152362793212 1104 28-29 0.05140488109699959 1104 30-31 -0.04723265314260772 1104 32-33 0.04977679352059283 1104 34-35 0.040351426973565196 1104 36-37 0.05816017167630605 1104 38-39 0.19427578960013392 1104 40-41 0.18569564011162498 1104 42-43 0.19865879493384853 1104 44-45 0.13933974846273856 1104 46-47 0.13280645044855532 1104 48-49 0.045846670274116263 1104 50-51 0.06106669351019178 1104 52-53 0.055182627031491904 1104 54-55 0.15976999904938793 1104 56-57 0.035422570052681124 1104 58-59 0.14638192787806759 1104 60-61 0.19304549321069686 1104 62-63 0.22004546749570153 1104 64-65 0.1864870886245953 1104 66-67 0.2613315626999224 1104 68-69 0.25923375236627777 1104 70-71 0.010332566182498226 1104 72-73 0.038559022767067574 1104 74-75 0.0784846424738106 1104 76-77 -0.08971386026480133 1104 78-79 0.14647769248794518 1104 80-81 0.3184263113487553 1104 82-83 0.33236355032493137 1104 84-85 0.09570658804078391 1104 86-87 0.03996886030756741 1104 88-89 0.14543829989815293 1104 90-91 0.3790916946583991 1104 92-93 0.35489836150140874 1104 94-95 0.4025103925034479 1104 96-97 0.2738396183921843 1104 98-99 0.2988413647071262 1104 100-101 0.7766981160549555 1105 1 -0.08044723893256389 1105 2 -0.040198817346450255 1105 3 -0.03622519350394526 1105 4 -0.02353571620051298 1105 5 -0.0294820817485828 1105 6 -0.015402074809124144 1105 7 -0.07227866862037047 1105 8 -0.057631903043116495 1105 9 -0.08209615612930321 1105 10-11 -0.06534129262401933 1105 12-13 -0.08034563561074037 1105 14-15 -0.06681055548729375 1105 16-17 -0.06750213417127782 1105 18-19 -0.040227847455192034 1105 20-21 -0.0019620579573782493 1105 22-23 -0.11443848201936646 1105 24-25 -0.08129358511065732 1105 26-27 -0.07998846233987678 1105 28-29 -0.030953516547810977 1105 30-31 -0.03203897311967552 1105 32-33 -0.11317791792831144 1105 34-35 -0.031099533243313715 1105 36-37 0.01034246095446889 1105 38-39 -0.013798972515445485 1105 40-41 0.00677641186921818 1105 42-43 -0.07731312645542943 1105 44-45 -0.033980735800135164 1105 46-47 0.03002098170738776 1105 48-49 -0.039645827096499886 1105 50-51 5.488776791366945E-4 1105 52-53 -0.03607418035280574 1105 54-55 -0.025442680977839416 1105 56-57 -0.014109282273331303 1105 58-59 -0.12959276258867192 1105 60-61 -0.1516721237096732 1105 62-63 -0.1519911650019239 1105 64-65 -0.032006141435651614 1105 66-67 -0.06331173052835481 1105 68-69 -0.07177870831696254 1105 70-71 -0.10030298916879588 1105 72-73 -0.1056938768289939 1105 74-75 -0.027756717591230995 1105 76-77 -0.21635046770021304 1105 78-79 -0.028100696871124597 1105 80-81 0.005433642873953204 1105 82-83 0.08846891149033453 1105 84-85 -0.09539087753421072 1105 86-87 -0.1109246176636347 1105 88-89 -0.04010576017445899 1105 90-91 0.182174289543525 1105 92-93 0.09471819953600757 1105 94-95 0.1617373718813404 1105 96-97 0.028174795132063224 1105 98-99 0.11515684346987243 1105 100-101 0.7183326586678298 1106 1 -0.038629537654870205 1106 2 -0.08371689489663225 1106 3 -0.10147246466655702 1106 4 -0.06950620428224141 1106 5 -0.008856680355087576 1106 6 0.036466902692410486 1106 7 0.04980336823058451 1106 8 0.05162916264944073 1106 9 -0.036909622266691144 1106 10-11 0.01987813794942639 1106 12-13 0.027187283444071397 1106 14-15 -0.01276447710974793 1106 16-17 0.022714525749634618 1106 18-19 -0.09036420952457291 1106 20-21 -0.04586014263208682 1106 22-23 0.05158090105717861 1106 24-25 0.011742564882510464 1106 26-27 -0.025664361110187883 1106 28-29 0.07628494087542492 1106 30-31 0.10825978493852517 1106 32-33 0.06982479909869 1106 34-35 0.09616311312566239 1106 36-37 0.03416209275134463 1106 38-39 0.1013283434372525 1106 40-41 0.07927158876325535 1106 42-43 0.2188040338427797 1106 44-45 0.19412376759550654 1106 46-47 0.12003878027712034 1106 48-49 -0.017285735473443253 1106 50-51 0.0565886767621393 1106 52-53 0.18229808681981297 1106 54-55 0.13604502536874463 1106 56-57 0.09554791012359232 1106 58-59 0.09866878701048165 1106 60-61 0.09928802868898856 1106 62-63 0.10062643132294369 1106 64-65 0.03569465745710687 1106 66-67 0.20991626480878267 1106 68-69 0.19097730572271843 1106 70-71 0.10086457913034508 1106 72-73 0.23277277128153884 1106 74-75 0.2609296356044055 1106 76-77 0.03898862441951323 1106 78-79 0.2508660284857065 1106 80-81 0.3843393691871064 1106 82-83 0.49942179890608074 1106 84-85 0.22722527600845766 1106 86-87 0.35181888375169734 1106 88-89 0.3664408959543657 1106 90-91 0.6031666427978877 1106 92-93 0.4416110787036338 1106 94-95 0.5161009581016209 1106 96-97 0.46243308378809544 1106 98-99 0.432406294127361 1106 100-101 0.8946596352475176 1107 1 -0.0862484805384085 1107 2 -0.0775069169539293 1107 3 -0.08043853664499068 1107 4 -0.08598665120961613 1107 5 -0.15647876530396587 1107 6 -0.04053317900128661 1107 7 -0.09700869227302888 1107 8 -0.04082139695239562 1107 9 -0.10313084880331758 1107 10-11 -0.06787047797079282 1107 12-13 -0.032740229108476626 1107 14-15 6.1332678669145935E-6 1107 16-17 0.00635771909348648 1107 18-19 -0.0052414176109962796 1107 20-21 -0.06175333754696766 1107 22-23 0.004140741691628591 1107 24-25 -0.06782599812532197 1107 26-27 -0.15996503162693188 1107 28-29 -0.0753753264514927 1107 30-31 -0.19270121574644605 1107 32-33 -0.09887598830288624 1107 34-35 -0.16176840241813295 1107 36-37 -0.217735871818725 1107 38-39 -0.19014330570715998 1107 40-41 -0.2193374189096673 1107 42-43 -0.2156198056353844 1107 44-45 -0.3483677959590423 1107 46-47 -0.3753056215050634 1107 48-49 -0.336497265596428 1107 50-51 -0.31394534866345225 1107 52-53 -0.3294943986167098 1107 54-55 -0.38869324058823906 1107 56-57 -0.29654036934427097 1107 58-59 -0.26870683433294573 1107 60-61 -0.3493047973474219 1107 62-63 -0.3916799074796842 1107 64-65 -0.412682937103348 1107 66-67 -0.34880815092417095 1107 68-69 -0.4819726537446414 1107 70-71 -0.48588517421752897 1107 72-73 -0.42778802454077436 1107 74-75 -0.3915150483352434 1107 76-77 -0.6518671799177937 1107 78-79 -0.44520686114610797 1107 80-81 -0.3150117943196875 1107 82-83 -0.24886074817776915 1107 84-85 -0.33533073130627145 1107 86-87 -0.4508609837788313 1107 88-89 -0.30674267607119887 1107 90-91 -0.029463862886103698 1107 92-93 0.14062305625671812 1107 94-95 0.07317720345922041 1107 96-97 0.11361874214504297 1107 98-99 0.0881647572803601 1107 100-101 0.5822035456106782 1108 1 -6.257491951728866E-4 1108 2 -0.034035230746404466 1108 3 -0.028681453018201353 1108 4 -0.03014510901698486 1108 5 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-1.1037202542590023 2316 50-51 -1.132081875273677 2316 52-53 -1.0467838401849079 2316 54-55 -1.110643418596112 2316 56-57 -1.1539569431537373 2316 58-59 -1.1634796689915419 2316 60-61 -1.0679851386576331 2316 62-63 -1.1332938918998892 2316 64-65 -1.0453121349434937 2316 66-67 -1.1981865130495493 2316 68-69 -1.2795422587414933 2316 70-71 -1.0686432351065775 2316 72-73 -0.974499805908188 2316 74-75 -1.28399917417633 2316 76-77 -1.2318676347285518 2316 78-79 -1.2908558382009012 2316 80-81 -1.4482630090434263 2316 82-83 -1.5339663274424424 2316 84-85 -1.6122999987324675 2316 86-87 -1.5279144543639447 2316 88-89 -1.8562200858733817 2316 90-91 -2.100518569040453 2316 92-93 -2.5209826405007334 2316 94-95 -2.783910895911877 2316 96-97 -2.8146015075460618 2316 98-99 -3.1663909305072124 2316 100-101 -3.881849538385289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 35.0 5 87.0 6 198.0 7 450.0 8 1052.0 9 1869.0 10 3307.0 11 5066.0 12 7366.0 13 10298.0 14 12479.0 15 16497.0 16 21824.0 17 31164.0 18 30234.0 19 20098.0 20 18359.0 21 19372.0 22 20962.0 23 23233.0 24 27293.0 25 32295.0 26 38847.0 27 48095.0 28 57788.0 29 70894.0 30 86865.0 31 106545.0 32 131299.0 33 168354.0 34 226664.0 35 324689.0 36 513705.0 37 836542.0 38 654925.0 39 50130.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.894460772766635 19.760099906580354 14.433987104371134 22.911452216281877 2 15.504441690992712 21.342278244280294 39.26920173356928 23.884078331157713 3 19.549077452435718 25.04313474953107 30.319362943132873 25.08842485490034 4 13.04197527862119 17.043310493930186 36.99273147692719 32.92198275052143 5 13.424004036604664 37.94693003338323 34.1677528673903 14.461313062621809 6 31.611222440459674 36.26574100581062 17.232539683416913 14.890496870312791 7 27.918655765355805 33.396907119637724 20.981753531191316 17.70268358381516 8 25.924398959681582 35.02069141604668 19.83214751918543 19.222762105086307 9 26.209734913338117 17.163181437854462 20.918281065033163 35.708802583774265 10-11 24.911367899345986 26.683028949192185 27.19453910202349 21.211064049438335 12-13 26.13069138288736 24.245266230658444 27.73931910651105 21.884723279943145 14-15 22.730385847980607 25.398127442507594 26.7166549571376 25.1548317523742 16-17 21.82412705246521 28.79071269293577 27.208042138964462 22.177118115634556 18-19 21.60999830887465 27.644386734945392 29.36749701289787 21.37811794328209 20-21 22.611257810401636 26.480259703798843 29.144003192137262 21.76447929366226 22-23 22.23197044841349 26.55298914695437 29.09349049377544 22.121549910856693 24-25 22.35750610960482 26.602921557205907 29.257242271944012 21.782330061245265 26-27 22.204600192545122 26.56301984143213 29.502192936401528 21.73018702962122 28-29 21.66801330351942 27.206490273818616 29.41695966610775 21.708536756554217 30-31 21.94738057947018 26.66085365354921 29.28624286107461 22.105522905906007 32-33 21.8724681477093 26.882578977211807 29.197914720859313 22.047038154219578 34-35 22.468572830771276 26.67482498013501 29.080164203444593 21.776437985649125 36-37 22.364503091643925 26.35778783377664 29.319313275069952 21.958395799509482 38-39 22.05874996787271 26.830944675008617 29.38236284963129 21.727942507487384 40-41 22.17337707520105 26.850693294970775 29.1296632093569 21.846266420471284 42-43 21.93272432203303 26.96263558441293 28.72090494521634 22.3837351483377 44-45 22.12004207930573 26.844168128214218 28.616566833780936 22.419222958699113 46-47 22.443509133968604 27.11148458439438 28.278972384146872 22.166033897490152 48-49 22.49148735832125 27.1703626741128 28.132040761201836 22.20610920636412 50-51 21.936181468775963 27.396048021060714 28.382032460128098 22.285738050035224 52-53 22.457053153285592 27.606060086899525 27.722463118545676 22.214423641269207 54-55 21.8901945678401 27.108504481482175 28.130156965170556 22.871143985507167 56-57 22.291684858238376 27.50630579746508 27.79215295731401 22.409856386982536 58-59 22.67973294522094 27.074307182630662 27.8193437035682 22.426616168580193 60-61 22.301229174953807 27.171325265947267 28.370283100008443 22.157162459090483 62-63 22.12309734581745 27.18575188206777 28.33720928947313 22.35394148264165 64-65 21.909236182284776 27.348118357937125 28.19585137640385 22.54679408337425 66-67 22.063856032947683 27.305276736004032 27.73949190179804 22.891375329250245 68-69 21.762931741395708 27.32648791311308 28.01207079050801 22.898509554983207 70-71 22.09099112255783 27.253635540054184 27.80267106654068 22.852702270847303 72-73 22.12370526669489 26.95436890628402 27.987862030698903 22.934063796322192 74-75 21.758759310860892 27.729495657786966 27.541565208356587 22.970179822995547 76-77 22.00571646040955 27.171807427691036 27.728249969323237 23.094226142576176 78-79 22.007394255228224 27.277269096702426 27.715649312959318 22.99968733511003 80-81 21.983745301047943 27.40504552222061 27.66320464937887 22.94800452735257 82-83 21.991178515413004 27.228333976255715 27.808553004621306 22.971934503709974 84-85 21.82916760157529 27.21215499108567 27.583705178270034 23.37497222906901 86-87 21.87741235877648 27.1273138753861 27.739675087140675 23.255598678696746 88-89 21.876200820731697 27.074875981975598 27.46678501234178 23.58213818495092 90-91 21.782856838654297 26.92946795555723 27.714109292478046 23.573565913310425 92-93 21.955034855972492 27.13246209060674 27.552300538396835 23.360202515023936 94-95 21.740231540482558 26.84118509201159 27.54448046529159 23.87410290221427 96-97 21.47818732994526 26.858718249597974 27.769692844855086 23.893401575601676 98-99 21.524488710909306 27.01723345156473 27.27613866343621 24.18213917408975 100-101 21.578739976887913 26.387540254100294 27.974147292840946 24.059572476170842 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1174.0 1 860.5 2 635.0 3 760.0 4 692.5 5 720.5 6 1263.0 7 2478.5 8 3843.5 9 4497.5 10 4248.0 11 3921.5 12 4186.5 13 4657.0 14 4947.5 15 5095.5 16 5304.0 17 5590.5 18 5915.5 19 6311.0 20 7144.5 21 8393.0 22 9773.5 23 11790.5 24 14204.5 25 17216.0 26 21741.0 27 26798.0 28 30793.5 29 35019.5 30 40583.5 31 46683.0 32 52524.0 33 58705.5 34 66452.5 35 72690.5 36 80503.0 37 90110.0 38 94639.5 39 96049.0 40 99019.0 41 105504.5 42 112126.0 43 118434.5 44 125613.0 45 130577.0 46 132097.5 47 143607.0 48 174605.5 49 175716.0 50 148957.0 51 136547.5 52 128437.0 53 124329.0 54 120302.0 55 110832.0 56 100244.0 57 89076.0 58 78720.0 59 69100.5 60 58739.0 61 46995.0 62 36849.0 63 28783.0 64 21674.5 65 16310.5 66 11508.0 67 8611.5 68 6098.5 69 3669.5 70 2452.0 71 1610.5 72 1065.5 73 788.5 74 581.5 75 414.5 76 270.5 77 127.5 78 65.0 79 38.0 80 20.5 81 14.0 82 11.0 83 6.0 84 4.0 85 3.0 86 2.0 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4068924963773442 2 0.0 3 0.0 4 0.0 5 2.486949044072053E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012296581384578485 16-17 0.0028599914006828607 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002445499893337519 36-37 0.00737794883074709 38-39 0.013070298864956456 40-41 0.0023764179754466284 42-43 5.802881102834791E-4 44-45 3.039604387199176E-4 46-47 2.9014405514173953E-4 48-49 2.6251128798538336E-4 50-51 5.941044938616571E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.486949044072053E-4 60-61 0.0022797032903993817 62-63 0.0 64-65 1.3816383578178074E-4 66-67 0.0017684970980067934 68-69 0.0020310083859921765 70-71 1.3816383578178073E-5 72-73 0.0 74-75 0.0 76-77 0.12690348316556557 78-79 4.144915073453422E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14751752746420727 88-89 0.43901558819660824 90-91 0.33921984961142804 92-93 0.0 94-95 0.0 96-97 5.664717267053009E-4 98-99 0.0 100-101 1.6579660293813687E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3618892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.918767123472406 #Duplication Level Percentage of deduplicated Percentage of total 1 70.04181163719335 31.46191825837208 2 15.333214332439463 13.774981677062753 3 5.675718655503067 7.648388536346706 4 2.8767983595858477 5.1688894230169655 5 1.559022158573557 3.501467664064944 6 1.0041986599546364 2.7064419451323216 7 0.6965417488062735 2.1901457624483647 8 0.4616889652015811 1.6590799289093423 9 0.3476688996558132 1.405517250574203 >10 1.779941212605661 14.406618861698265 >50 0.13517866311289609 4.21168922626298 >100 0.08044934402239595 6.601616091321033 >500 0.004746722052139639 1.4533085581737524 >1k 0.002650766849306093 2.1666990776202617 >5k 1.8493722204461114E-4 0.5361159001853858 >10k+ 1.8493722204461114E-4 1.1071218388106037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14353 0.3966131069951797 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13979 0.38627845207870254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11650 0.32192173737154905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7467 0.20633387235651132 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6661 0.18406186202848826 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5233 0.1446022705292117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4736 0.1308687852525027 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3881 0.1072427693338182 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.7632767156356143E-5 0.0 3 0.0 0.0 0.0 2.7632767156356143E-5 0.0 4 0.0 0.0 0.0 5.5265534312712287E-5 0.0 5 0.0 0.0 0.0 5.5265534312712287E-5 0.0 6 0.0 0.0 0.0 5.5265534312712287E-5 0.0 7 0.0 0.0 0.0 5.5265534312712287E-5 0.0 8 0.0 0.0 0.0 5.5265534312712287E-5 0.0 9 0.0 0.0 0.0 1.1053106862542457E-4 0.0 10-11 0.0 0.0 0.0 1.93429370094493E-4 0.0 12-13 0.0 0.0 0.0 3.454095894544518E-4 0.0 14-15 0.0 0.0 0.0 3.5922597303262986E-4 0.0 16-17 0.0 0.0 0.0 5.388389595489448E-4 0.0 18-19 0.0 0.0 0.0 7.875338639561501E-4 0.0 20-21 0.0 0.0 0.0 8.566157818470405E-4 0.0 22-23 0.0 0.0 0.0 0.001160576220566958 0.0 24-25 0.0 0.0 0.0 0.0014921694264432319 0.0 26-27 0.0 0.0 0.0 0.001920477317366752 0.0 28-29 0.0 0.0 0.0 0.005443655129802161 0.0 30-31 0.0 0.0 0.0 0.015750677279123004 0.0 32-33 0.0 0.0 0.0 0.03466530639764878 0.0 34-35 0.0 0.0 0.0 0.06311324018511744 0.0 36-37 0.0 0.0 0.0 0.10819609980071249 0.0 38-39 0.0 0.0 0.0 0.1771122210886647 0.0 40-41 0.0 0.0 0.0 0.2506568308753066 0.0 42-43 0.0 2.7632767156356143E-5 0.0 0.32938258450376523 0.0 44-45 0.0 2.7632767156356143E-5 0.0 0.4242873233022704 0.0 46-47 0.0 2.7632767156356143E-5 0.0 0.5405660074962171 0.0 48-49 0.0 2.7632767156356143E-5 0.0 0.6661569342218557 0.0 50-51 0.0 2.7632767156356143E-5 0.0 0.8034503378382113 0.0 52-53 0.0 2.7632767156356143E-5 0.0 0.9409233544410831 0.0 54-55 0.0 4.144915073453422E-5 0.0 1.0955148702973174 0.0 56-57 0.0 5.5265534312712287E-5 0.0 1.2813866785745471 0.0 58-59 0.0 5.5265534312712287E-5 0.0 1.487126446437197 0.0 60-61 0.0 5.5265534312712287E-5 0.0 1.715179673778604 0.0 62-63 0.0 5.5265534312712287E-5 0.0 1.946396852959414 0.0 64-65 0.0 5.5265534312712287E-5 0.0 2.1877276249194506 0.0 66-67 0.0 5.5265534312712287E-5 0.0 2.466694225746444 0.0 68-69 0.0 5.5265534312712287E-5 0.0 2.7868612824035646 0.0 70-71 0.0 5.5265534312712287E-5 0.0 3.142011422280632 0.0 72-73 0.0 5.5265534312712287E-5 0.0 3.4990544067079092 0.0 74-75 0.0 5.5265534312712287E-5 0.0 3.8799721019582787 0.0 76-77 0.0 5.5265534312712287E-5 0.0 4.286325759376075 0.0 78-79 0.0 5.5265534312712287E-5 0.0 4.748235095161723 0.0 80-81 0.0 5.5265534312712287E-5 0.0 5.2525469121488015 0.0 82-83 0.0 5.5265534312712287E-5 0.0 5.761597748703194 0.0 84-85 0.0 5.5265534312712287E-5 0.0 6.2892178047866585 0.0 86-87 0.0 5.5265534312712287E-5 0.0 6.852470314118244 0.0 88-89 0.0 5.5265534312712287E-5 0.0 7.45985235259853 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15315 0.0 25.294086 1 TACATGG 15420 0.0 24.372187 2 ACATGGG 15250 0.0 23.64816 3 GAGTACT 12800 0.0 21.151217 12-13 CATGGGA 10070 0.0 19.697018 4 GTACTTT 13825 0.0 19.377085 14-15 CATGGGG 6275 0.0 19.056383 4 AGTACTT 13345 0.0 18.936108 12-13 GTGTAGC 1885 0.0 17.947025 1 TCGCGCA 270 4.9326627E-7 17.57478 9 AGTACAT 11355 0.0 17.467983 2 GCACCGT 720 0.0 17.13541 6 GGACCGT 360 1.9372237E-9 17.13541 6 GTACCGT 280 7.2187686E-7 16.94711 6 TTTGCGT 730 0.0 16.900679 3 GTTCGCG 260 6.694916E-6 16.42566 7 AGAGTAC 24390 0.0 16.363186 10-11 ACTTTTT 16675 0.0 16.278254 16-17 GTATCAA 35130 0.0 16.276031 1 ATGGGAG 4435 0.0 15.942129 5 >>END_MODULE