FastQCFastQC Report
Fri 27 May 2016
SRR939025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331314
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT172990.7420278864194184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160630.6890105751520387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135120.5795873056997041No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA109580.4700353534530312No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT100170.42967185029558436No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA91190.3911528005236532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87480.3752390282904834No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT73180.31390022965589365No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT65160.279499029302788No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC59600.2556498180854231No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.21451421816194643No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48230.2068790390312073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47640.2043482774092207No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43390.1861182148779615No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC41610.17848303574722238No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40380.17320704117935207No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39700.1702902311743506No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG39120.16780236381714345No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39090.16767368102280517No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA39010.16733052690456968No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC36340.15587775820846098No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT35870.15386172776382762No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30350.13018409360558036No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA28910.12400731947734196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.12284917432829727No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG27850.11946052741072202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.11688687152395602No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC26000.11152508842652684No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA23820.10217413870461035No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG23550.10101599355556566No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG23380.1002867910543153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6700.026.863653
GTACATG105900.023.977851
TACATGG108700.022.876312
CTTTGCG7950.022.6398052
GAGTACT114450.022.38002412-13
TTGCGTT8300.022.2557754
ACATGGG109950.021.840823
GTATATG6100.021.7861581
GTAAGCG2100.021.51798494-95
AGTACTT117200.020.46119112-13
GTACTTT123950.020.4168914-15
GTATTAG4000.020.1716481
CATGGGG40150.020.054864
CATGGGA67400.019.606524
ACTTTTT141750.018.18826316-17
TGTACCG2352.5994686E-618.1397325
TACTTTT132350.018.0836314-15
GTGTAGG13150.016.9638541
ATGGGAG26350.016.896765
ACTTGAC12650.016.8412028
Produced by FastQC (version 0.11.5)