##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939025_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2331314 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93727528767039 33.0 31.0 34.0 30.0 34.0 2 32.0015158833173 33.0 31.0 34.0 30.0 34.0 3 32.039122572077375 34.0 31.0 34.0 30.0 34.0 4 35.574068100650535 37.0 35.0 37.0 33.0 37.0 5 35.62018158000166 37.0 35.0 37.0 33.0 37.0 6 35.72066825832985 37.0 35.0 37.0 33.0 37.0 7 35.65833903112151 37.0 35.0 37.0 33.0 37.0 8 35.65445452650308 37.0 35.0 37.0 33.0 37.0 9 37.26713990479189 39.0 37.0 39.0 34.0 39.0 10-11 37.16013415610253 39.0 37.0 39.0 33.5 39.0 12-13 37.0612043251145 39.0 37.0 39.0 33.0 39.0 14-15 38.258668287497954 40.0 38.0 41.0 33.0 41.0 16-17 38.186899748382245 40.0 38.0 41.0 33.0 41.0 18-19 38.13939435013902 40.0 38.0 41.0 33.0 41.0 20-21 38.242619183859404 40.0 38.0 41.0 34.0 41.0 22-23 38.17321175954848 40.0 38.0 41.0 33.0 41.0 24-25 38.07392354697823 40.0 38.0 41.0 33.0 41.0 26-27 37.940869183644935 40.0 38.0 41.0 33.0 41.0 28-29 37.81080283479617 40.0 38.0 41.0 33.0 41.0 30-31 37.59927470087685 40.0 37.0 41.0 32.5 41.0 32-33 37.36817884677912 40.0 36.5 41.0 32.0 41.0 34-35 37.01465675580381 40.0 36.0 41.0 31.0 41.0 36-37 36.71839464782522 39.0 35.5 41.0 30.5 41.0 38-39 36.463990693660314 39.0 35.0 41.0 30.0 41.0 40-41 36.30149113332652 39.0 35.0 41.0 30.0 41.0 42-43 36.06364457983781 39.0 35.0 41.0 30.0 41.0 44-45 35.955187503699634 39.0 35.0 41.0 29.0 41.0 46-47 35.657386992914724 39.0 35.0 41.0 27.5 41.0 48-49 35.34937700369834 39.0 35.0 41.0 26.0 41.0 50-51 34.17924698260295 38.0 34.0 40.0 24.5 40.5 52-53 34.26591677483171 38.0 33.5 39.5 24.0 40.5 54-55 34.73533037591676 38.0 34.5 40.0 24.5 41.0 56-57 34.618111717254735 38.0 34.0 40.0 24.0 41.0 58-59 34.40275140972001 38.0 34.0 40.0 24.0 41.0 60-61 34.05623802713834 37.5 34.0 40.0 23.0 41.0 62-63 33.66496812527184 37.0 33.5 40.0 22.0 41.0 64-65 33.25088169161254 36.0 33.0 39.5 21.5 41.0 66-67 32.812304563006094 36.0 33.0 39.0 20.0 41.0 68-69 32.361747924132054 35.0 32.0 39.0 18.5 40.0 70-71 31.886932862754655 35.0 32.0 37.5 15.5 40.0 72-73 31.447792532451658 35.0 31.0 37.0 12.0 39.0 74-75 30.992100806669544 35.0 31.0 36.5 7.5 39.0 76-77 30.574545299346205 35.0 31.0 36.0 6.0 39.0 78-79 30.198891269043983 34.5 30.5 36.0 2.0 37.5 80-81 29.761536626983755 34.0 30.0 35.0 2.0 37.0 82-83 29.326944590046644 34.0 29.5 35.0 2.0 36.5 84-85 28.936211938846505 34.0 29.0 35.0 2.0 36.0 86-87 28.506107499890618 34.0 29.0 35.0 2.0 36.0 88-89 28.026718194117137 34.0 28.0 35.0 2.0 35.0 90-91 27.565401528923175 34.0 27.0 35.0 2.0 35.0 92-93 27.082645666778475 33.0 25.5 35.0 2.0 35.0 94-95 26.558324833119862 33.0 25.0 35.0 2.0 35.0 96-97 25.903181210253102 33.0 23.5 35.0 2.0 35.0 98-99 25.07395872027535 32.5 17.5 35.0 2.0 35.0 100-101 22.051539818317053 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22737813015448793 1101 2 0.28936101494829813 1101 3 0.37514198284869593 1101 4 0.2671636275425584 1101 5 0.21484475495304878 1101 6 0.2859717816401215 1101 7 0.3750838461049071 1101 8 0.38882908593839005 1101 9 0.41043069831441414 1101 10-11 0.3541946329170784 1101 12-13 0.3384052948066696 1101 14-15 0.4241471184253953 1101 16-17 0.5137041442024497 1101 18-19 0.6842108196375065 1101 20-21 0.4811384974891837 1101 22-23 0.49268391267059286 1101 24-25 0.6443766928044354 1101 26-27 0.6632399841832424 1101 28-29 0.6323994711585499 1101 30-31 0.4662302486348153 1101 32-33 0.4117546675435264 1101 34-35 0.45278134502412826 1101 36-37 0.3702046121624889 1101 38-39 0.5823512682139764 1101 40-41 0.46983459001692296 1101 42-43 0.5537665913540124 1101 44-45 0.6980879114125216 1101 46-47 0.7341681964390219 1101 48-49 0.5807469373408125 1101 50-51 0.6124590731992541 1101 52-53 0.6374589562351929 1101 54-55 0.7373987440473044 1101 56-57 0.6821956838894536 1101 58-59 0.5661251383715111 1101 60-61 0.6155410428614729 1101 62-63 0.6675564789490238 1101 64-65 0.4845022255503224 1101 66-67 0.522288137399805 1101 68-69 0.5091825460785557 1101 70-71 0.3110274948879592 1101 72-73 0.2058420292803973 1101 74-75 0.11257508022958262 1101 76-77 0.048167767100789405 1101 78-79 -0.06931224065683494 1101 80-81 -0.027999431564815325 1101 82-83 -0.08532720049374376 1101 84-85 -0.1890639915995358 1101 86-87 -0.32630505792405984 1101 88-89 -0.2814509099028868 1101 90-91 -0.11350278392367485 1101 92-93 -0.15988844591406348 1101 94-95 -0.5026358149287908 1101 96-97 -0.8401261601706196 1101 98-99 -0.7095389810455721 1101 100-101 -0.6539341891879822 1102 1 0.22391952212875665 1102 2 0.30277869741949814 1102 3 0.36619395651656816 1102 4 0.12108100872534777 1102 5 0.37743440622968194 1102 6 0.31223114761426984 1102 7 0.35515589390323754 1102 8 0.38156897186271266 1102 9 0.37037023149382975 1102 10-11 0.4392243020923772 1102 12-13 0.3504667437011406 1102 14-15 0.4720379252291522 1102 16-17 0.6259966026680033 1102 18-19 0.6272154603069495 1102 20-21 0.4435852818507655 1102 22-23 0.5329940371377901 1102 24-25 0.5743105792768617 1102 26-27 0.5697543059488126 1102 28-29 0.5457004070915232 1102 30-31 0.5872439501775943 1102 32-33 0.592846542864585 1102 34-35 0.5556204709522277 1102 36-37 0.5652407166666507 1102 38-39 0.671307054411173 1102 40-41 0.5750988371684116 1102 42-43 0.471520082095104 1102 44-45 0.5409369190778364 1102 46-47 0.6162015817226347 1102 48-49 0.6174963904250035 1102 50-51 0.426225034623009 1102 52-53 0.46764622561230595 1102 54-55 0.5514410499079787 1102 56-57 0.5312730616389061 1102 58-59 0.3873564298422352 1102 60-61 0.36254526002789333 1102 62-63 0.4021133235552199 1102 64-65 0.3954529811241585 1102 66-67 0.42500356733162903 1102 68-69 0.44147064316009477 1102 70-71 0.47472731664198875 1102 72-73 0.18374645615052998 1102 74-75 0.19196501366727858 1102 76-77 0.1816703418858907 1102 78-79 0.11356884779821996 1102 80-81 0.15065730137078148 1102 82-83 0.26073653061146373 1102 84-85 0.31609941216223447 1102 86-87 0.1615412331349262 1102 88-89 0.11944665944246324 1102 90-91 0.04478408534765066 1102 92-93 0.0443812880415102 1102 94-95 -0.026991486884742955 1102 96-97 -0.1678360240004544 1102 98-99 -0.36230560604453643 1102 100-101 -0.17306676611238458 1103 1 0.24142161470456713 1103 2 0.1735460068742256 1103 3 0.27980370699174273 1103 4 0.23391196270249281 1103 5 -1.6490964156190557E-4 1103 6 0.18014957536136933 1103 7 0.14025429353061014 1103 8 0.0726952848757918 1103 9 0.24378892839430932 1103 10-11 0.24960207516002697 1103 12-13 0.2948352002198078 1103 14-15 0.37583091584482275 1103 16-17 0.4421189765878779 1103 18-19 0.44517690511022323 1103 20-21 0.4809475269051049 1103 22-23 0.4644235977335569 1103 24-25 0.45222815229470115 1103 26-27 0.4283503570132723 1103 28-29 0.40919032279708745 1103 30-31 0.30374007389404767 1103 32-33 0.34440663961303386 1103 34-35 0.23002581896734142 1103 36-37 0.2589015393737881 1103 38-39 0.45151245276034047 1103 40-41 0.40237834183985655 1103 42-43 0.2895436890267007 1103 44-45 0.42542608667945103 1103 46-47 0.4591721673805367 1103 48-49 0.3368405324861854 1103 50-51 0.17369926647825906 1103 52-53 0.3004693834225307 1103 54-55 0.3369245093858311 1103 56-57 0.22359707482591062 1103 58-59 0.2873808414280532 1103 60-61 0.30033823682976646 1103 62-63 0.30662992019595947 1103 64-65 0.2672918318500095 1103 66-67 0.2688598553978352 1103 68-69 0.1918396228766781 1103 70-71 0.1883640903757211 1103 72-73 0.1595571862835783 1103 74-75 0.12981788626988333 1103 76-77 0.22949525016437633 1103 78-79 0.052182127269219336 1103 80-81 -0.018711727125808153 1103 82-83 -9.650583647733413E-4 1103 84-85 -0.12101226339635573 1103 86-87 0.09276855097291303 1103 88-89 -0.0404305574036421 1103 90-91 -0.13828597266602216 1103 92-93 -0.27838682175062246 1103 94-95 -0.3794010032159001 1103 96-97 -0.33610761033911984 1103 98-99 -0.4125948757819877 1103 100-101 -0.01825716568968261 1104 1 0.07050982767603031 1104 2 0.15014554946361613 1104 3 0.229122577630406 1104 4 0.2481369509122544 1104 5 0.25133147891066443 1104 6 0.18130789250953683 1104 7 0.16234578072361927 1104 8 0.17318595325986053 1104 9 0.26477661245306194 1104 10-11 0.29823935158918147 1104 12-13 0.24267712141198672 1104 14-15 0.22507557981030146 1104 16-17 0.3019109401234985 1104 18-19 0.34473050295061824 1104 20-21 0.37899079074785647 1104 22-23 0.3503216463854173 1104 24-25 0.42366291465585704 1104 26-27 0.4159250707935698 1104 28-29 0.46893366743890397 1104 30-31 0.3190278740803194 1104 32-33 0.39321305739152734 1104 34-35 0.5926779722286284 1104 36-37 0.4908280308180437 1104 38-39 0.5636579215047917 1104 40-41 0.5774336165144192 1104 42-43 0.5781455271209239 1104 44-45 0.6890619854676387 1104 46-47 0.7854180450223467 1104 48-49 0.8223982277520037 1104 50-51 0.5384475680920318 1104 52-53 0.6419464342904391 1104 54-55 0.7297608397221538 1104 56-57 0.8153807141983762 1104 58-59 0.7471475975146049 1104 60-61 0.5553041888134871 1104 62-63 0.7045246439155548 1104 64-65 0.734875115740401 1104 66-67 0.7004674821196772 1104 68-69 0.8110200405977039 1104 70-71 0.8105117185512682 1104 72-73 0.7069049316008602 1104 74-75 0.6789558253189902 1104 76-77 0.7643686889083732 1104 78-79 0.6318735864023033 1104 80-81 0.5443031978815043 1104 82-83 0.6599812288505476 1104 84-85 0.6978938727686668 1104 86-87 0.666263689506124 1104 88-89 0.5559064811922774 1104 90-91 0.45055190265663114 1104 92-93 0.3136330450154361 1104 94-95 0.32004834185527287 1104 96-97 0.13745404374283865 1104 98-99 0.35048119789053445 1104 100-101 0.5295467647284653 1105 1 0.1916551552167043 1105 2 0.25779952339666323 1105 3 0.2184956077011151 1105 4 0.18491819292605527 1105 5 0.27328820435575807 1105 6 0.23569923759855982 1105 7 0.36529109122707126 1105 8 0.3397567855283654 1105 9 0.3796607593139498 1105 10-11 0.34820101782991486 1105 12-13 0.33596658388749745 1105 14-15 0.46520463679983237 1105 16-17 0.41125266429080654 1105 18-19 0.42142464214475694 1105 20-21 0.4279781406073013 1105 22-23 0.35733352985078426 1105 24-25 0.3924794663212481 1105 26-27 0.3252923508003889 1105 28-29 0.3426345789305145 1105 30-31 0.38631426985509165 1105 32-33 0.32769959798272197 1105 34-35 0.3567549804916794 1105 36-37 0.3658386790845043 1105 38-39 0.449167760503002 1105 40-41 0.49431324851033054 1105 42-43 0.39860510630143864 1105 44-45 0.4235155164327935 1105 46-47 0.40011052308692285 1105 48-49 0.3301874346691207 1105 50-51 0.31885369297490485 1105 52-53 0.22826128223319841 1105 54-55 0.44257176910285523 1105 56-57 0.5082421163621049 1105 58-59 0.3760768078391621 1105 60-61 0.44256566764472893 1105 62-63 0.47726401694563236 1105 64-65 0.5540941718780559 1105 66-67 0.41060569661835444 1105 68-69 0.45709828405966135 1105 70-71 0.3950003887957507 1105 72-73 0.4017469113265655 1105 74-75 0.28217479627051745 1105 76-77 0.2786320418345163 1105 78-79 0.37058642395235 1105 80-81 0.42416413152150767 1105 82-83 0.25226975427860765 1105 84-85 0.42199728873254827 1105 86-87 0.4764743122167445 1105 88-89 0.5866373782022443 1105 90-91 0.6198433670062329 1105 92-93 0.6002311368460873 1105 94-95 0.6374678409608201 1105 96-97 0.757358848240667 1105 98-99 0.7224943591478841 1105 100-101 0.7047213962984458 1106 1 0.14264258839068233 1106 2 0.168345946492245 1106 3 0.21332678886524548 1106 4 0.13782554682588 1106 5 0.10401142213654424 1106 6 0.14408158131518434 1106 7 0.23143498047193845 1106 8 0.14088043144035822 1106 9 0.16063158969951274 1106 10-11 0.16735220722001287 1106 12-13 0.23471825826004533 1106 14-15 0.23383801134237814 1106 16-17 0.19700959978607813 1106 18-19 0.36573393915045926 1106 20-21 0.1522244645307964 1106 22-23 0.19094288156390604 1106 24-25 0.259602967723076 1106 26-27 0.2603190534199129 1106 28-29 0.23860036337058688 1106 30-31 0.19012465346604301 1106 32-33 0.2197889171731191 1106 34-35 0.1620714061360431 1106 36-37 0.025052849496923102 1106 38-39 0.19105216507746547 1106 40-41 0.2591857857832309 1106 42-43 0.15113611842043895 1106 44-45 0.18153295213966913 1106 46-47 0.0774407693020649 1106 48-49 0.03705432731156577 1106 50-51 0.02553041977208892 1106 52-53 0.14926146723795597 1106 54-55 0.08711617302622443 1106 56-57 0.03026453211148805 1106 58-59 -0.016456363992155332 1106 60-61 0.21350564669869954 1106 62-63 0.11845411768191383 1106 64-65 0.025610484455121707 1106 66-67 -0.04757389616471386 1106 68-69 -0.011929396782498713 1106 70-71 0.13568285788685586 1106 72-73 0.09961209128539039 1106 74-75 -0.022873054855431008 1106 76-77 -0.08717875942669195 1106 78-79 0.012012643971452519 1106 80-81 -0.010103197986275347 1106 82-83 0.20331356229606712 1106 84-85 0.18620494030743018 1106 86-87 0.22347403907665608 1106 88-89 0.07143095893379936 1106 90-91 0.04000924402808437 1106 92-93 0.16660258989576704 1106 94-95 0.12406819572532513 1106 96-97 0.13604048282528325 1106 98-99 0.17289898760193623 1106 100-101 0.6193299841721327 1107 1 0.03198139506779185 1107 2 0.0854407383567164 1107 3 0.13538371001452276 1107 4 0.18320749528283642 1107 5 0.21329247183545874 1107 6 0.135377772331708 1107 7 -0.028488842568485495 1107 8 0.0967435157729426 1107 9 0.20544909232091868 1107 10-11 0.21190936016760276 1107 12-13 0.2686540155746613 1107 14-15 0.18726580999007325 1107 16-17 0.20940282496492557 1107 18-19 0.34011949940263264 1107 20-21 0.2637939781840615 1107 22-23 0.23066788649506265 1107 24-25 0.37369767964374745 1107 26-27 0.3512938432480368 1107 28-29 0.2210964450596009 1107 30-31 0.27704650539811837 1107 32-33 0.3173869446323607 1107 34-35 0.17459035407559043 1107 36-37 0.16609888222277647 1107 38-39 0.37360625925513347 1107 40-41 0.22410971625419052 1107 42-43 0.2461920103658386 1107 44-45 -0.01779288738156737 1107 46-47 0.11993370743814324 1107 48-49 0.22486825917427922 1107 50-51 0.14759415453365676 1107 52-53 0.3712479875126249 1107 54-55 0.35504289340041595 1107 56-57 0.3800294140956666 1107 58-59 0.38704362603906617 1107 60-61 0.5008987799324913 1107 62-63 0.3922039601655243 1107 64-65 0.30906701447982954 1107 66-67 0.28929322426530035 1107 68-69 0.30249722709778126 1107 70-71 0.27431809134695584 1107 72-73 0.4123686058826834 1107 74-75 0.3481030667241889 1107 76-77 0.26336078381739014 1107 78-79 0.2762095891116161 1107 80-81 0.2888663012267685 1107 82-83 0.21730392142182353 1107 84-85 0.22629453156618595 1107 86-87 0.2767988786228628 1107 88-89 0.328285268998453 1107 90-91 0.21704769970737559 1107 92-93 0.23959360502527716 1107 94-95 0.429358868877312 1107 96-97 0.609817076479839 1107 98-99 0.4741018805223369 1107 100-101 0.6237319261290608 1108 1 0.1359860181522201 1108 2 0.19730672162479124 1108 3 0.2227123212201505 1108 4 0.055829258604561005 1108 5 0.12312635070553313 1108 6 0.19321265441429603 1108 7 0.21225420563204267 1108 8 0.14296617304007952 1108 9 0.18235903003547804 1108 10-11 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66-67 -1.6688342966100684 2316 68-69 -1.8910601884952385 2316 70-71 -1.6884153783631177 2316 72-73 -1.8311899184643252 2316 74-75 -1.8916598231568287 2316 76-77 -1.8558469005868794 2316 78-79 -1.7213579278461602 2316 80-81 -1.6029619663328134 2316 82-83 -1.5724080273734522 2316 84-85 -1.562728449823993 2316 86-87 -1.5038603248975555 2316 88-89 -1.709692326779411 2316 90-91 -1.9294110016706831 2316 92-93 -1.8589685412514 2316 94-95 -1.9161397285488988 2316 96-97 -2.1120609186958497 2316 98-99 -2.217530768956177 2316 100-101 -1.94951513382496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6589.0 3 1562.0 4 1548.0 5 1712.0 6 2084.0 7 2572.0 8 3230.0 9 4074.0 10 5049.0 11 6738.0 12 9848.0 13 14845.0 14 13339.0 15 14386.0 16 18485.0 17 25664.0 18 21739.0 19 15224.0 20 15184.0 21 16306.0 22 17121.0 23 18704.0 24 21098.0 25 24564.0 26 28124.0 27 32961.0 28 39158.0 29 47250.0 30 56355.0 31 69387.0 32 86141.0 33 111616.0 34 153225.0 35 227316.0 36 330680.0 37 461425.0 38 358233.0 39 47777.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.431065014022344 18.344765084295446 14.145506200858854 22.078663700823352 2 15.801006584486165 21.257024217531466 38.44009886388957 24.5018703340928 3 20.389449183416463 24.603687453118955 30.58149025797402 24.42537310549056 4 12.950122803703005 16.35277295913986 37.016728784157465 33.68037545299967 5 13.629888944095004 37.510150744187506 33.66358232913791 15.196377982579584 6 31.046460075391234 35.50126417448398 17.09062613869052 16.361649611434267 7 27.624402345124764 33.832244607157264 20.721005691133676 17.822347356584288 8 26.00982528065486 32.675324519251404 19.886321691671945 21.428528508421792 9 26.33338269284008 17.314799911582103 21.303348506016494 35.04846888956132 10-11 25.293859241568146 25.424787635689867 26.812787418513118 22.468565704228872 12-13 27.654614961031072 23.496941757552687 27.046228367940195 21.802214913476053 14-15 23.790870487411492 24.695877478881812 27.372271545445415 24.14098048826128 16-17 21.841457065687873 28.075193255800677 27.694711844882043 22.388637833629407 18-19 21.460760630316017 27.228992613806103 29.703204461247378 21.607042294630503 20-21 22.815429067748287 25.615487186332945 29.1476291459122 22.42145460000657 22-23 22.246077093592305 25.582052779654184 29.30706177889368 22.86480834785983 24-25 22.9535290895071 25.668160483883433 29.404148970700117 21.97416145590935 26-27 22.873278982007392 25.122462209514484 30.379106938165705 21.62515187031241 28-29 21.611455141919595 26.375959262096664 30.242823095659073 21.769762500324664 30-31 21.956475078022038 25.992650688783286 29.726547240359398 22.32432699283528 32-33 21.982211145741807 26.27972481833194 29.621391266798046 22.11667276912821 34-35 22.947302425242956 25.586150763486476 29.185298149262568 22.281248662008 36-37 23.01436908203231 25.302046432472558 29.82407853638308 21.85950594911205 38-39 22.759161161982515 25.60662854759081 29.76431706291199 21.869893227514687 40-41 22.276558052217403 26.070527040794673 29.813781949708613 21.83913295727931 42-43 21.942418136562974 26.57920199984915 28.652105983384875 22.826273880203 44-45 22.230857378327812 26.246291175308684 28.50248073954293 23.02037070682057 46-47 22.83536295612097 26.60693346758074 27.95573628574181 22.601967290556484 48-49 23.092172238894825 26.334762198214534 28.023060141869106 22.550005421021535 50-51 22.291699311510808 26.80930748242459 28.505689848152173 22.39330335791243 52-53 22.55855055845572 27.3101013453804 27.8149961849995 22.31635191116437 54-55 21.78975487961655 26.366427332964797 28.33801548072022 23.505802306698435 56-57 22.640651607546985 26.9981617999579 27.489741869827167 22.871444722667952 58-59 23.294033398696783 26.33237808769043 27.52770621693857 22.84588229667422 60-61 22.488143891870056 26.291057063940503 28.74210902838704 22.478690015802403 62-63 22.618805951751387 26.189503364162693 28.5579721669466 22.63371851713932 64-65 22.205543496769995 26.922839818852374 28.462012669987562 22.409604014390073 66-67 22.183984561604404 26.475783517546684 27.97340446630439 23.366827454544513 68-69 21.538132175674548 26.848258003370614 28.46806617989633 23.14554364105851 70-71 22.404194518194174 26.79677520543028 27.642793312830726 23.156236963544817 72-73 22.1972333891145 26.08066763225645 28.228476160933454 23.4936228176956 74-75 21.626742892619706 27.464818790904623 27.66206637866363 23.24637193781204 76-77 22.320492236075044 26.328724905981616 27.71409790842771 23.63668494951563 78-79 22.058848562248826 26.35892593843131 28.2382557240814 23.343969775238456 80-81 22.204837276694374 27.11078941419869 27.798944505630548 22.885428803476398 82-83 21.886915412732172 26.646583026256497 28.087773511015868 23.378728049995466 84-85 21.922073488788673 26.40671192603451 27.666458774135183 24.004755811041637 86-87 22.072265445691656 26.460327578872235 27.919566848683665 23.547840126752448 88-89 22.23771819908731 26.141152616190734 27.685664001531062 23.935465183190896 90-91 21.974404975275156 25.894404302754253 28.160541086177304 23.970649635793286 92-93 22.072345992563505 26.345539449878668 27.994295338254066 23.58781921930376 94-95 21.92116001791024 26.09919574224344 27.906345049795338 24.073299190050978 96-97 21.24518626059346 25.86622519839666 28.19195247318156 24.696636067828322 98-99 21.903421896351283 25.997285143370686 27.470731866626686 24.628561093651342 100-101 22.09733767535286 25.02682152532473 28.37743241816148 24.49840838116092 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7291.0 1 4544.0 2 2022.5 3 2188.5 4 1838.5 5 1801.0 6 3062.5 7 5708.0 8 8454.0 9 9191.5 10 7184.0 11 5268.5 12 5092.5 13 5227.0 14 5436.5 15 5802.5 16 6053.0 17 6066.5 18 6098.5 19 6337.0 20 6772.5 21 7490.0 22 8368.5 23 9396.0 24 10676.0 25 12299.0 26 14396.5 27 16255.0 28 17501.0 29 18835.0 30 20775.5 31 22988.0 32 25594.5 33 28637.5 34 31601.0 35 34007.0 36 37732.5 37 42650.0 38 45630.5 39 46620.5 40 48522.5 41 53328.5 42 57508.0 43 61218.5 44 64695.5 45 67644.0 46 73930.0 47 92994.5 48 145297.0 49 148097.0 50 99291.0 51 81525.0 52 77200.0 53 77064.5 54 77352.0 55 74293.0 56 67750.5 57 60721.5 58 55805.0 59 51378.0 60 45907.0 61 39705.5 62 34309.0 63 29340.0 64 23853.5 65 18649.5 66 13234.0 67 10128.0 68 7117.0 69 3877.5 70 2756.0 71 2094.0 72 1594.5 73 1180.0 74 855.0 75 640.0 76 433.5 77 246.5 78 151.0 79 99.5 80 67.5 81 44.0 82 31.5 83 24.5 84 20.5 85 17.5 86 14.0 87 11.5 88 6.5 89 7.5 90 5.0 91 3.0 92 6.5 93 4.5 94 2.5 95 4.0 96 3.5 97 1.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32269355393567745 2 0.0879761370626179 3 0.0978418179618876 4 0.10268886988196357 5 0.11439900416674888 6 0.057263843480543586 7 0.0589367198069415 8 0.06030933627988336 9 0.06301167496098767 10-11 0.060673937530508544 12-13 0.05923697966039753 14-15 0.05713516068620529 16-17 0.0588080370126032 18-19 0.06700084158547498 20-21 0.06839490519080656 22-23 0.08366526345228484 24-25 0.09256582339401728 26-27 0.0868179919135732 28-29 0.08512366845478558 30-31 0.0790970242532752 32-33 0.07090421968040342 34-35 0.3175247950297558 36-37 0.49208300554965995 38-39 0.46608908109332337 40-41 0.44865256246048363 42-43 0.47779921537810865 44-45 0.4864424097311645 46-47 0.48125220369285304 48-49 0.49931068916499455 50-51 0.49433495445058023 52-53 0.4943135073181905 54-55 0.48605636134814956 56-57 0.46486659454710955 58-59 0.44803059562118186 60-61 0.4081389293763088 62-63 0.4771129071416377 64-65 0.500104233063414 66-67 0.5105275394048163 68-69 0.5211867642025055 70-71 0.5188704739044161 72-73 0.5075892822674252 74-75 0.5101843852865808 76-77 0.4842333550950237 78-79 0.4646521232232123 80-81 0.47095758014578903 82-83 0.47970801016079345 84-85 0.48063023685355133 86-87 0.48045865979443353 88-89 0.4871501651000251 90-91 0.5011551425505101 92-93 0.5064740313831598 94-95 0.5134657965422076 96-97 0.5053587804988946 98-99 0.5084900618277932 100-101 0.48680701098178963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2331314.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.01543040974838 #Duplication Level Percentage of deduplicated Percentage of total 1 65.01319229600105 22.764649105562146 2 15.075646705561738 10.557605162010992 3 6.881727601897528 7.228999618292624 4 3.682674553849142 5.158017382482232 5 2.2269325665968402 3.8988501156437003 6 1.5107912525028324 3.174060357940171 7 1.064797313154313 2.6099035353469326 8 0.7243489011759887 2.029071083320443 9 0.5636178670979529 1.7761789982753264 >10 2.9495981224383976 18.11998506727773 >50 0.18284071361439194 4.388981611487696 >100 0.11004848282770145 7.087584400464464 >500 0.006276471315992247 1.487219150643107 >1k 0.0061534032509727886 4.956664590995075 >5k 7.384083901167347E-4 1.8384255738507247 >10k+ 6.15340325097279E-4 2.923804246406587 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17299 0.7420278864194184 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16063 0.6890105751520387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13512 0.5795873056997041 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10958 0.4700353534530312 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10017 0.42967185029558436 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9119 0.3911528005236532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8748 0.3752390282904834 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7318 0.31390022965589365 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6516 0.279499029302788 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5960 0.2556498180854231 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5001 0.21451421816194643 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4823 0.2068790390312073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4764 0.2043482774092207 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4339 0.1861182148779615 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4161 0.17848303574722238 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4038 0.17320704117935207 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3970 0.1702902311743506 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3912 0.16780236381714345 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3909 0.16767368102280517 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3901 0.16733052690456968 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3634 0.15587775820846098 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3587 0.15386172776382762 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3035 0.13018409360558036 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2891 0.12400731947734196 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2864 0.12284917432829727 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2785 0.11946052741072202 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2725 0.11688687152395602 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2600 0.11152508842652684 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2382 0.10217413870461035 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2355 0.10101599355556566 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2338 0.1002867910543153 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.2894264779433404E-5 0.0 9 0.0 0.0 0.0 4.2894264779433404E-5 0.0 10-11 0.0 0.0 0.0 4.2894264779433404E-5 0.0 12-13 0.0 0.0 0.0 1.286827943383002E-4 0.0 14-15 0.0 0.0 0.0 1.501299267280169E-4 0.0 16-17 2.1447132389716702E-5 0.0 0.0 2.7881272106631714E-4 0.0 18-19 4.2894264779433404E-5 0.0 0.0 5.361783097429175E-4 0.0 20-21 4.2894264779433404E-5 0.0 0.0 8.57885295588668E-4 0.0 22-23 4.2894264779433404E-5 0.0 0.0 0.001415510737721302 0.0 24-25 4.2894264779433404E-5 0.0 0.0 0.002444973092427704 0.0 26-27 4.2894264779433404E-5 0.0 0.0 0.004182190815994757 0.0 28-29 4.2894264779433404E-5 0.0 0.0 0.011860264211513337 0.0 30-31 4.2894264779433404E-5 0.0 0.0 0.029897302551265083 0.0 32-33 4.2894264779433404E-5 0.0 0.0 0.061832082679553244 0.0 34-35 4.2894264779433404E-5 0.0 0.0 0.10828657143567962 0.0 36-37 4.2894264779433404E-5 0.0 0.0 0.1787618484682887 0.0 38-39 4.2894264779433404E-5 0.0 0.0 0.27632485370911 0.0 40-41 4.2894264779433404E-5 0.0 0.0 0.38111554256526575 0.0 42-43 4.2894264779433404E-5 0.0 0.0 0.49188998135815254 0.0 44-45 4.2894264779433404E-5 0.0 0.0 0.6270069154133677 0.0 46-47 4.2894264779433404E-5 0.0 0.0 0.7963534727625707 0.0 48-49 4.2894264779433404E-5 0.0 0.0 0.9720269341667404 0.0 50-51 4.2894264779433404E-5 0.0 0.0 1.1588314572811726 0.0 52-53 4.2894264779433404E-5 0.0 0.0 1.334890967068357 0.0 54-55 4.2894264779433404E-5 0.0 0.0 1.5329766818197808 0.0 56-57 4.2894264779433404E-5 0.0 0.0 1.772562597745306 0.0 58-59 4.2894264779433404E-5 0.0 0.0 2.041145036661728 2.1447132389716702E-5 60-61 4.2894264779433404E-5 0.0 0.0 2.3521927977097894 4.2894264779433404E-5 62-63 4.2894264779433404E-5 0.0 0.0 2.6546831529343535 4.2894264779433404E-5 64-65 4.2894264779433404E-5 0.0 0.0 2.9606264964736626 4.2894264779433404E-5 66-67 4.2894264779433404E-5 0.0 0.0 3.3172923081146513 4.2894264779433404E-5 68-69 4.2894264779433404E-5 0.0 0.0 3.7244446694010334 4.2894264779433404E-5 70-71 4.2894264779433404E-5 0.0 0.0 4.169601349281993 4.2894264779433404E-5 72-73 4.2894264779433404E-5 0.0 0.0 4.622693468147148 4.2894264779433404E-5 74-75 4.2894264779433404E-5 0.0 0.0 5.103795541913273 4.2894264779433404E-5 76-77 4.2894264779433404E-5 0.0 0.0 5.590881365616129 4.2894264779433404E-5 78-79 4.2894264779433404E-5 0.0 0.0 6.170575906977781 4.2894264779433404E-5 80-81 4.2894264779433404E-5 0.0 0.0 6.8032019710772555 4.2894264779433404E-5 82-83 4.2894264779433404E-5 0.0 0.0 7.42435381934823 4.2894264779433404E-5 84-85 4.2894264779433404E-5 0.0 0.0 8.097086021016473 4.2894264779433404E-5 86-87 4.2894264779433404E-5 0.0 0.0 8.799608289574035 4.2894264779433404E-5 88-89 4.2894264779433404E-5 0.0 0.0 9.534687305099183 4.2894264779433404E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 670 0.0 26.86365 3 GTACATG 10590 0.0 23.97785 1 TACATGG 10870 0.0 22.87631 2 CTTTGCG 795 0.0 22.639805 2 GAGTACT 11445 0.0 22.380024 12-13 TTGCGTT 830 0.0 22.255775 4 ACATGGG 10995 0.0 21.84082 3 GTATATG 610 0.0 21.786158 1 GTAAGCG 210 0.0 21.517984 94-95 AGTACTT 11720 0.0 20.461191 12-13 GTACTTT 12395 0.0 20.41689 14-15 GTATTAG 400 0.0 20.171648 1 CATGGGG 4015 0.0 20.05486 4 CATGGGA 6740 0.0 19.60652 4 ACTTTTT 14175 0.0 18.188263 16-17 TGTACCG 235 2.5994686E-6 18.139732 5 TACTTTT 13235 0.0 18.08363 14-15 GTGTAGG 1315 0.0 16.963854 1 ATGGGAG 2635 0.0 16.89676 5 ACTTGAC 1265 0.0 16.841202 8 >>END_MODULE