##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939025_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2331314 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13700042122168 33.0 31.0 34.0 30.0 34.0 2 32.365365197480905 34.0 31.0 34.0 30.0 34.0 3 32.43948305547858 34.0 31.0 34.0 30.0 34.0 4 35.999131391138214 37.0 35.0 37.0 35.0 37.0 5 35.930397192313 37.0 35.0 37.0 35.0 37.0 6 35.98549187282365 37.0 35.0 37.0 35.0 37.0 7 35.92128387681797 37.0 35.0 37.0 35.0 37.0 8 35.9038516476116 37.0 35.0 37.0 35.0 37.0 9 37.549124227796 39.0 37.0 39.0 35.0 39.0 10-11 37.45983552623113 39.0 37.0 39.0 34.0 39.0 12-13 37.36793756653973 39.0 37.0 39.0 34.0 39.0 14-15 38.60918928123796 40.0 38.0 41.0 34.0 41.0 16-17 38.70981064755756 40.0 38.0 41.0 34.0 41.0 18-19 38.7758894769216 40.0 38.0 41.0 34.0 41.0 20-21 38.750600734178235 40.0 38.0 41.0 34.0 41.0 22-23 38.67686613643636 40.0 38.0 41.0 34.0 41.0 24-25 38.57339315939423 40.0 38.0 41.0 34.0 41.0 26-27 38.37400431687881 40.0 38.0 41.0 34.0 41.0 28-29 38.1985225928382 40.0 38.0 41.0 33.5 41.0 30-31 37.98240541600145 40.0 37.0 41.0 33.0 41.0 32-33 37.847723858733744 40.0 37.0 41.0 33.0 41.0 34-35 37.7122521891088 40.0 37.0 41.0 33.0 41.0 36-37 37.53627739549456 40.0 36.5 41.0 33.0 41.0 38-39 37.36299378805258 40.0 36.0 41.0 32.0 41.0 40-41 37.16414755798661 40.0 36.0 41.0 31.5 41.0 42-43 36.94627815043361 40.0 35.5 41.0 31.0 41.0 44-45 36.64801588288836 39.0 35.0 41.0 30.5 41.0 46-47 36.2505644885245 39.0 35.0 41.0 30.0 41.0 48-49 35.83920184067869 39.0 35.0 41.0 28.5 41.0 50-51 35.53513361992421 39.0 35.0 40.0 28.0 41.0 52-53 35.23228252393285 38.0 35.0 40.0 27.0 41.0 54-55 34.91214589712068 38.0 34.0 40.0 26.0 41.0 56-57 34.76932686888166 38.0 34.0 40.0 26.0 41.0 58-59 34.479951006170765 38.0 34.0 40.0 26.0 41.0 60-61 34.07118539158603 37.0 33.5 40.0 25.5 41.0 62-63 33.632827452672615 36.5 33.0 39.5 23.5 41.0 64-65 33.193386219102194 36.0 33.0 39.0 22.5 40.5 66-67 32.718737587472134 35.0 32.0 39.0 22.0 40.0 68-69 32.2299441430884 35.0 31.5 38.0 20.5 40.0 70-71 31.681757798391807 35.0 31.0 37.0 19.0 39.0 72-73 31.167887937875378 35.0 31.0 36.5 17.5 39.0 74-75 30.659793361168852 34.0 30.0 36.0 13.5 38.5 76-77 28.788019546058575 32.5 28.0 34.5 8.0 36.0 78-79 29.751368327046464 34.0 29.5 35.0 7.5 37.0 80-81 29.757977046421033 34.0 30.0 35.0 7.0 37.0 82-83 29.374368703658106 34.0 29.0 35.0 2.0 36.0 84-85 28.94679180925435 34.0 29.0 35.0 2.0 36.0 86-87 28.267693884221515 33.5 28.0 35.0 2.0 35.0 88-89 27.574075392675546 33.0 26.5 35.0 2.0 35.0 90-91 26.981661843921497 32.5 25.5 34.5 2.0 35.0 92-93 26.599644663910567 32.0 25.0 34.0 2.0 35.0 94-95 26.045000373180102 32.0 24.5 34.0 2.0 35.0 96-97 25.159889229850634 32.0 21.0 34.0 2.0 35.0 98-99 23.943552863320857 31.0 4.5 34.0 2.0 35.0 100-101 18.478057653323404 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10847873100286165 1101 2 0.12792681403859518 1101 3 0.09568614784790697 1101 4 0.10447203708555008 1101 5 0.09461045849451466 1101 6 0.06908925905878505 1101 7 0.12716047653385942 1101 8 0.13907826174100535 1101 9 0.17887073654758012 1101 10-11 0.1581941718864357 1101 12-13 0.16649154247960496 1101 14-15 0.3104925188145913 1101 16-17 0.19583401207688667 1101 18-19 0.22976417495861057 1101 20-21 0.20542748039670045 1101 22-23 0.2139091700618465 1101 24-25 0.17907611893409126 1101 26-27 0.17062309061000036 1101 28-29 0.12094724393038092 1101 30-31 0.14678149253209227 1101 32-33 0.2540833129583504 1101 34-35 0.2527610944474432 1101 36-37 0.24199795638909194 1101 38-39 0.34972347407597937 1101 40-41 0.30833708627385903 1101 42-43 0.3703788332370621 1101 44-45 0.3626718241039839 1101 46-47 0.38051594411523837 1101 48-49 0.3668332964703467 1101 50-51 0.12970271732631744 1101 52-53 0.08326925277066977 1101 54-55 0.217019537898679 1101 56-57 0.13680531280001418 1101 58-59 0.1566269984329054 1101 60-61 0.12972085682681467 1101 62-63 0.18929760077878655 1101 64-65 0.19523054126931783 1101 66-67 0.04562446235494377 1101 68-69 0.1593477727171404 1101 70-71 0.12566438420631343 1101 72-73 0.10364949865509843 1101 74-75 0.2214940375008574 1101 76-77 -0.16141860944482644 1101 78-79 0.1461072596769526 1101 80-81 0.1878602875145141 1101 82-83 0.06040051117138745 1101 84-85 0.08812545141051231 1101 86-87 0.3727667664237835 1101 88-89 0.4031390074647234 1101 90-91 0.3289808756540644 1101 92-93 0.32223599267478065 1101 94-95 0.17147565523744746 1101 96-97 0.47062819039101456 1101 98-99 0.6364113944210636 1101 100-101 1.020983649834271 1102 1 0.18161643963365037 1102 2 0.18057059266153885 1102 3 0.22664029754576376 1102 4 0.16623638030948484 1102 5 0.15604541047790121 1102 6 0.12550469809679043 1102 7 0.23642120669315858 1102 8 0.08691244897901385 1102 9 0.14692828241727085 1102 10-11 0.2749948461799363 1102 12-13 0.31265465737048004 1102 14-15 0.3769083151288086 1102 16-17 0.3569262621022631 1102 18-19 0.33446288000661895 1102 20-21 0.43432193272494146 1102 22-23 0.38754475616693895 1102 24-25 0.28948045975294434 1102 26-27 0.36783803538686755 1102 28-29 0.3806372719591309 1102 30-31 0.20668516737935505 1102 32-33 0.33233181424999003 1102 34-35 0.2656627959971942 1102 36-37 0.29410407788129334 1102 38-39 0.3733037041531446 1102 40-41 0.29464403969681285 1102 42-43 0.3546659778725001 1102 44-45 0.21985033106411578 1102 46-47 0.2875513663089677 1102 48-49 0.38928342267163174 1102 50-51 0.24757456670563727 1102 52-53 0.25913367603317994 1102 54-55 0.2190260577538723 1102 56-57 0.23513927281906888 1102 58-59 0.10346821921958593 1102 60-61 0.1615059133570611 1102 62-63 0.3317636693418393 1102 64-65 0.241807159806811 1102 66-67 0.21819769635387587 1102 68-69 0.2568948536615494 1102 70-71 0.30266381300006984 1102 72-73 0.3856943429197095 1102 74-75 0.37242570058241853 1102 76-77 0.28771713068116966 1102 78-79 0.30596690676647853 1102 80-81 0.40614603267287563 1102 82-83 0.4781129369330941 1102 84-85 0.6194159853772376 1102 86-87 0.610544870010358 1102 88-89 0.44611149327497657 1102 90-91 0.48538953951509356 1102 92-93 0.5256667810831708 1102 94-95 0.5894613755171854 1102 96-97 0.8076320284960907 1102 98-99 1.140437396286746 1102 100-101 1.0989655010740407 1103 1 0.10651142232970301 1103 2 0.08080339846576123 1103 3 0.0924251397424527 1103 4 0.11262240798976109 1103 5 0.1030215466958424 1103 6 0.11368115911185583 1103 7 0.17223103892879976 1103 8 0.21381600227850583 1103 9 0.08754424003996775 1103 10-11 0.15578957604016352 1103 12-13 0.2252807137489441 1103 14-15 0.12272963872455733 1103 16-17 0.19781808575348236 1103 18-19 0.23817741251935587 1103 20-21 0.26923680298524033 1103 22-23 0.21515730307452685 1103 24-25 0.1880034832670674 1103 26-27 0.1378890989852053 1103 28-29 0.059272342709562054 1103 30-31 0.13247696828096878 1103 32-33 0.13346566873794785 1103 34-35 0.12507936557151567 1103 36-37 0.04728136124951732 1103 38-39 -0.03323251413607409 1103 40-41 0.10367701713756361 1103 42-43 0.24990412141780638 1103 44-45 0.0754363753209617 1103 46-47 -0.037744562114788494 1103 48-49 -0.09788840769655138 1103 50-51 -0.04933129177278062 1103 52-53 0.0016939700600531182 1103 54-55 0.0917414376572907 1103 56-57 0.11053960340071001 1103 58-59 0.04790369428285857 1103 60-61 0.011142739755932496 1103 62-63 -0.009587139607610595 1103 64-65 -0.03138158038700567 1103 66-67 0.04489475483332939 1103 68-69 0.09958193252931835 1103 70-71 0.06631476746933629 1103 72-73 -0.11332930156817511 1103 74-75 0.031454663385723336 1103 76-77 -0.11453065619597425 1103 78-79 0.053808715373605764 1103 80-81 -0.012155015924523838 1103 82-83 -0.04650013619417592 1103 84-85 0.14788634807167256 1103 86-87 0.13850066842030273 1103 88-89 0.19855491818377047 1103 90-91 0.12244678004325138 1103 92-93 0.29599897722367885 1103 94-95 0.39464289109031014 1103 96-97 0.09661987795840687 1103 98-99 0.28866223584154227 1103 100-101 1.2542039770140612 1104 1 0.02938677033195347 1104 2 0.0502182898349659 1104 3 0.02541452768107888 1104 4 0.11210684909856639 1104 5 0.05503611419264587 1104 6 0.09952272870977197 1104 7 0.10588558235491519 1104 8 0.09652783359440065 1104 9 0.06459722090005471 1104 10-11 0.14170383098995387 1104 12-13 0.16967737039179553 1104 14-15 0.19880204347717267 1104 16-17 0.14702634365463751 1104 18-19 0.21171392254063903 1104 20-21 0.15506501876269851 1104 22-23 0.002441085282164579 1104 24-25 0.13124615430161413 1104 26-27 0.04211854128570991 1104 28-29 0.12038561606902931 1104 30-31 0.04445954999705748 1104 32-33 0.11863225764819418 1104 34-35 0.2635922908864714 1104 36-37 0.21112153968361014 1104 38-39 0.30740368976154997 1104 40-41 0.3658361777738861 1104 42-43 0.24125699443310822 1104 44-45 0.3603080180606213 1104 46-47 0.34587287797473465 1104 48-49 0.15631540456563897 1104 50-51 0.13988431495551623 1104 52-53 0.22343352492143254 1104 54-55 0.5412600873662967 1104 56-57 0.3266299851002401 1104 58-59 0.4512280560463111 1104 60-61 0.20980385021907466 1104 62-63 0.2770865293630109 1104 64-65 0.3482632037628548 1104 66-67 0.17924380257070283 1104 68-69 0.25542228507018194 1104 70-71 0.17576527888685334 1104 72-73 0.14697268493939575 1104 74-75 0.14606667996474698 1104 76-77 0.017059623236942656 1104 78-79 0.3239482627974155 1104 80-81 0.2811288998213186 1104 82-83 0.21274284164017487 1104 84-85 0.20502263395161435 1104 86-87 0.31086838107989223 1104 88-89 0.3639712488919784 1104 90-91 0.43521491292559844 1104 92-93 0.40111287770560367 1104 94-95 0.6511419968914431 1104 96-97 0.7291993331901487 1104 98-99 0.8456105751502854 1104 100-101 1.2233731327314388 1105 1 6.144449499601023E-5 1105 2 0.00269507661407431 1105 3 0.03296414860279384 1105 4 0.016634491998303247 1105 5 0.05595186510839767 1105 6 -0.05572899630939787 1105 7 0.04311405156012427 1105 8 0.0538176368144363 1105 9 0.07809922277414927 1105 10-11 0.06134136399958123 1105 12-13 0.10615513681142374 1105 14-15 -0.021361513895691076 1105 16-17 -0.021168349249357732 1105 18-19 0.011553133717058017 1105 20-21 0.10087599073646203 1105 22-23 0.09006566999977395 1105 24-25 -0.07435057455092675 1105 26-27 -0.06300114522932887 1105 28-29 0.02487599003614349 1105 30-31 0.09174317722254699 1105 32-33 0.05783598028214243 1105 34-35 0.14595024301186044 1105 36-37 0.21440120575396548 1105 38-39 0.2102435824270259 1105 40-41 0.32098567943501166 1105 42-43 0.3790189417880825 1105 44-45 0.25806336349039327 1105 46-47 0.26838544292915856 1105 48-49 0.29518477163267676 1105 50-51 0.15860396739609683 1105 52-53 0.08993620828457694 1105 54-55 0.2858028508044015 1105 56-57 0.19051319491367735 1105 58-59 0.1873693372457339 1105 60-61 0.18951890260854753 1105 62-63 0.18459036273893759 1105 64-65 -0.0743824645223441 1105 66-67 0.04974490999041592 1105 68-69 0.03944349641697187 1105 70-71 0.25586686334890274 1105 72-73 0.29404121718234677 1105 74-75 0.275629462143808 1105 76-77 -0.0625042237687623 1105 78-79 0.1734669611533235 1105 80-81 0.3227316768892088 1105 82-83 0.4726617018497308 1105 84-85 0.3475656529481199 1105 86-87 0.2752765504299255 1105 88-89 0.5788053489004987 1105 90-91 0.48477726917399977 1105 92-93 0.841977729907665 1105 94-95 0.9600535310936777 1105 96-97 0.8391799533451874 1105 98-99 0.925813318143728 1105 100-101 1.0197889262402562 1106 1 -0.045047561425946014 1106 2 -0.0063906028258600145 1106 3 -0.0932410621795654 1106 4 -0.06525373971341963 1106 5 -0.030155547230705793 1106 6 -0.04953371861565614 1106 7 -0.03176543728678638 1106 8 -0.040473258965946 1106 9 -0.04527215960847286 1106 10-11 0.0594959811389657 1106 12-13 -0.015865532111831726 1106 14-15 -0.09078375909131609 1106 16-17 -0.1630708771449818 1106 18-19 -0.13910982650214976 1106 20-21 -0.047809780650048594 1106 22-23 -0.1256225953780543 1106 24-25 -0.03950724897509161 1106 26-27 -0.0739855055773262 1106 28-29 -0.2746996304642906 1106 30-31 -0.17613856582409682 1106 32-33 -0.1450537068533535 1106 34-35 -0.1342728266857094 1106 36-37 -0.21236936133725948 1106 38-39 -0.09543312610789201 1106 40-41 -0.1031770548878157 1106 42-43 -0.07485601483902116 1106 44-45 -0.013993889530226511 1106 46-47 0.1568081145091682 1106 48-49 0.06060815464733338 1106 50-51 -0.08076044197419208 1106 52-53 -0.061959455378335804 1106 54-55 0.027458282202971418 1106 56-57 0.013896169869752839 1106 58-59 -0.09781064936723283 1106 60-61 -0.11059495869504588 1106 62-63 0.04912399589115779 1106 64-65 0.05117245053028796 1106 66-67 0.04723086987534231 1106 68-69 -0.02452557443826464 1106 70-71 0.007006468899682261 1106 72-73 0.17645321883071574 1106 74-75 0.049114447776165804 1106 76-77 -0.050072479014996674 1106 78-79 -0.02850792504492361 1106 80-81 -0.021991359856865955 1106 82-83 -0.09375600239195947 1106 84-85 -0.012003321672878542 1106 86-87 0.15371680540301824 1106 88-89 0.338372596545657 1106 90-91 0.40729213109136353 1106 92-93 0.43686770765151195 1106 94-95 0.6511685183061147 1106 96-97 0.5981369036090776 1106 98-99 0.605814918077666 1106 100-101 1.039607165010743 1107 1 -0.052629447205418955 1107 2 0.051236878021015286 1107 3 2.713143019335007E-4 1107 4 -0.02246580313653368 1107 5 -0.03868398312535959 1107 6 -0.031214392869046037 1107 7 0.017381031955466142 1107 8 0.05507642630129794 1107 9 0.045943139119067666 1107 10-11 0.06256026776439683 1107 12-13 0.012955467131511966 1107 14-15 -0.035490811509099274 1107 16-17 0.04667843418657469 1107 18-19 0.05897911010782764 1107 20-21 0.13717892172265067 1107 22-23 0.11881293846169427 1107 24-25 0.05597226769977226 1107 26-27 0.029553624010802082 1107 28-29 0.09993048426958495 1107 30-31 0.07895127029885174 1107 32-33 0.1282601225148241 1107 34-35 0.10977808648350873 1107 36-37 0.1220279214251363 1107 38-39 0.07075745875748396 1107 40-41 -0.024038883928476196 1107 42-43 -0.007620082480379153 1107 44-45 -0.05686864159314098 1107 46-47 -0.1283173633873318 1107 48-49 -0.2585609950196641 1107 50-51 -0.3223718109508482 1107 52-53 -0.27902797999318807 1107 54-55 -0.19490230925728724 1107 56-57 -0.1927846931129622 1107 58-59 -0.06598740221254928 1107 60-61 -0.005625454449223355 1107 62-63 -0.11893510600118873 1107 64-65 -0.03250596710117293 1107 66-67 -0.12251479462509707 1107 68-69 -0.18661463570638404 1107 70-71 0.026206241108230444 1107 72-73 0.006676389796830051 1107 74-75 -0.15988488228315134 1107 76-77 -0.17425104675885095 1107 78-79 -0.03441166844738319 1107 80-81 -0.11552483530821434 1107 82-83 -0.22748524144115834 1107 84-85 0.13649597054379115 1107 86-87 0.07737695790791932 1107 88-89 0.23716161246066747 1107 90-91 0.3321716685554854 1107 92-93 0.4365203489231497 1107 94-95 0.5545784989705531 1107 96-97 0.6591694484797124 1107 98-99 0.5831716584920059 1107 100-101 0.7258400291146039 1108 1 0.06876405970924537 1108 2 0.0717384363551119 1108 3 0.09363797090452408 1108 4 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Count 2 3.0 3 8.0 4 25.0 5 84.0 6 200.0 7 446.0 8 920.0 9 1764.0 10 2952.0 11 4411.0 12 6283.0 13 8566.0 14 10613.0 15 13706.0 16 18076.0 17 25237.0 18 24881.0 19 16995.0 20 15731.0 21 17035.0 22 17825.0 23 19354.0 24 22477.0 25 26351.0 26 31080.0 27 36863.0 28 44772.0 29 54125.0 30 65319.0 31 79357.0 32 98047.0 33 124788.0 34 171293.0 35 248688.0 36 368830.0 37 486158.0 38 257960.0 39 10091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35951617008028 18.989407123611404 14.677414423519552 21.973662282788762 2 16.17662828773816 21.758802117604066 37.70290059597291 24.361668998684863 3 20.407160940139338 24.702077883974447 30.843163983916366 24.04759719196985 4 13.259775388471908 16.96022071672885 36.4765964601937 33.303407434605546 5 13.526706398194325 37.82948157133702 33.66350478742889 14.980307243039762 6 31.143123577518946 35.80830381493012 16.772901462437062 16.275671145113872 7 27.611038238521278 34.06216408428895 20.37426103905351 17.95253663813626 8 26.152676130285325 32.99465451672319 19.653251342376016 21.199418010615474 9 26.03814844332424 17.347513033422356 21.093211811021597 35.52112671223181 10-11 25.387592576546957 25.622996301656492 26.57825157829447 22.411159543502077 12-13 27.562117329540335 23.60878886327625 27.04781509483493 21.781278712348488 14-15 23.639120978391208 24.90000735642163 27.240271668575833 24.220599996611327 16-17 21.765091563754897 28.26355193545765 27.649266303899054 22.3220901968884 18-19 21.41135695603638 27.302806049408 29.676457688352325 21.60937930620329 20-21 22.789229593268 25.60195666478218 29.206855018242933 22.401958723706887 22-23 22.166276185876292 25.622009733566564 29.405090862921085 22.806623217636062 24-25 22.918045985664303 25.76901390568021 29.447605394984418 21.865334713671068 26-27 22.814858916473714 25.275359732751575 30.464021577530954 21.445759773243758 28-29 21.50029983091081 26.47547691988295 30.28217134199855 21.742051907207696 30-31 21.93477583886169 26.06276975130763 29.782753417171605 22.219700992659075 32-33 21.951676179184787 26.289079892283922 29.72184356118481 22.037400367346482 34-35 22.921650740154355 25.568664328723727 29.307113594296613 22.202571336825304 36-37 22.95586380291238 25.33497944858714 29.906102782807174 21.8030539656933 38-39 22.683548658884174 25.65316227407119 29.86672495242475 21.796564114619887 40-41 22.26507404039191 26.06925307572196 29.839414711988884 21.826258171897255 42-43 21.969362732744205 26.6539206209043 28.725081562753292 22.651635083598205 44-45 22.26116147596167 26.258859722815902 28.548108935636492 22.931869865585938 46-47 22.85067062958345 26.72661085897251 27.8951537128002 22.527564798643834 48-49 23.071307023033775 26.339797591742382 28.07903041700322 22.509864968220626 50-51 22.256671341461715 26.915259384638546 28.476107607934043 22.351961665965696 52-53 22.587819572996175 27.379580785771456 27.740450235360832 22.292149405871537 54-55 21.785632480223597 26.43534933518179 28.196008774450803 23.583009410143806 56-57 22.63822033411201 27.029349113847385 27.410507550677426 22.92192300136318 58-59 23.28534992613538 26.460413367351666 27.496126611338262 22.758110095174693 60-61 22.50400693355536 26.45191690457332 28.611553823739534 22.43252233813179 62-63 22.613122771211653 26.34947658660991 28.38172620361273 22.65567443856571 64-65 22.121536690321605 27.03822251657447 28.276203450784454 22.564037342319466 66-67 22.056905215882498 26.603209872836185 27.86371659778359 23.476168313497723 68-69 21.4680599166109 26.998121171565348 28.23220689418507 23.301612017638682 70-71 22.271472029483828 26.964837825224595 27.447849108141682 23.315841037149898 72-73 22.10648158077376 26.260683888999935 27.954642746536933 23.67819178368937 74-75 21.554132133209 27.606791706308115 27.30809749351653 23.530978666966355 76-77 22.099743065682148 26.496971377873336 27.49398987504049 23.90929568140402 78-79 21.820477519528815 26.48954877769697 27.997891246032953 23.692082456741268 80-81 21.945606106782474 27.188946812939253 27.538642981551785 23.326804098726488 82-83 21.605884063665385 26.77457862818994 27.85851669916622 23.761020608978455 84-85 21.538302829375507 26.516963963815186 27.42443678113095 24.52029642567836 86-87 21.64582271600634 26.581564899900524 27.62242430981829 24.150188074274848 88-89 21.69823736972629 26.30872535265422 27.337150724931753 24.655886552687736 90-91 21.5293529238383 26.014359375373413 27.778783718232948 24.67750398255534 92-93 21.399348178752412 26.55869179355505 27.642737100193283 24.399222927499256 94-95 21.26151051401744 26.274909733549094 27.57629083437621 24.88728891805726 96-97 20.533184290061314 26.10896687447508 27.818646480053737 25.539202355409866 98-99 21.041073832182196 26.20901774707311 27.092468024470318 25.657440396274378 100-101 21.167330279961526 25.134769082948267 28.029245453989777 25.668655183100437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5585.0 1 3692.5 2 1952.5 3 2007.0 4 1676.5 5 1713.5 6 2814.0 7 5366.0 8 7851.5 9 8426.5 10 6767.0 11 4924.0 12 4542.0 13 4700.0 14 4901.5 15 4988.0 16 5172.0 17 5326.0 18 5422.0 19 5729.0 20 6210.0 21 7052.0 22 8081.5 23 9112.5 24 10566.0 25 12334.5 26 14399.0 27 16344.0 28 17622.5 29 18925.5 30 20844.0 31 23072.5 32 25790.0 33 28783.0 34 31644.0 35 34153.5 36 38049.5 37 42929.0 38 46023.5 39 47010.5 40 48896.5 41 53686.0 42 57941.5 43 61628.5 44 64914.5 45 68167.0 46 73617.0 47 92967.0 48 144400.5 49 147936.0 50 103614.5 51 85465.0 52 78154.0 53 77467.0 54 78580.0 55 75476.0 56 68389.5 57 61244.5 58 56318.0 59 51783.0 60 46085.0 61 39405.5 62 34123.5 63 29329.5 64 23732.5 65 18278.5 66 12452.0 67 9438.5 68 6524.5 69 3343.0 70 2363.5 71 1810.5 72 1337.5 73 943.5 74 687.0 75 509.5 76 302.0 77 142.0 78 75.5 79 31.5 80 16.5 81 8.5 82 4.0 83 2.5 84 2.5 85 1.0 86 2.0 87 2.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3900375496393879 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.506496336400846E-4 16-17 0.0029811514021706215 18-19 4.932840449634841E-4 20-21 0.0 22-23 0.0 24-25 2.14471323897167E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.014905757010853107 36-37 0.024621307983394774 38-39 0.01158145149044702 40-41 0.0066057167760327435 42-43 0.005726384348054359 44-45 0.0020374775770230866 46-47 0.0014798521348904524 48-49 0.003646012506251839 50-51 7.506496336400846E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.436738251475348E-4 60-61 0.0018230062531259196 62-63 0.0013082750757727186 64-65 7.720967660298012E-4 66-67 0.0029597042697809047 68-69 0.0031741755936780715 70-71 8.57885295588668E-5 72-73 0.0 74-75 0.0 76-77 0.09091439420000909 78-79 0.0034100940499649556 80-81 1.930241915074503E-4 82-83 0.0 84-85 1.930241915074503E-4 86-87 0.09355239148394424 88-89 0.4330390500807699 90-91 0.3859840416177315 92-93 0.0 94-95 9.222266927578181E-4 96-97 0.0 98-99 0.0 100-101 4.932840449634841E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2331314.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.838119046358976 #Duplication Level Percentage of deduplicated Percentage of total 1 63.50263456759697 21.48809708255777 2 15.579592262146685 10.543681953205052 3 7.090123042482832 7.19749282694601 4 3.8828624006676034 5.255550406176863 5 2.4519395806692077 4.148451171258208 6 1.5964711619765863 3.2412948739825658 7 1.1291013553647615 2.6744666254766654 8 0.7925160697953358 2.145380249270969 9 0.593724273476568 1.8081461381951787 >10 3.0530484429461247 18.186462698597282 >50 0.1955302444150427 4.569835517003461 >100 0.11723472983290871 7.296719976632763 >500 0.007230387598942308 1.6242170501250086 >1k 0.006722991977963897 5.37038954660255 >5k 7.610934314676109E-4 1.9901380425223572 >10k+ 5.073956209784073E-4 2.4596758414472375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17714 0.7598290063028833 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15199 0.6519499303826083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13806 0.5921982195448575 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10585 0.4540357926903026 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9470 0.4062086874612343 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9215 0.39527064994247885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9113 0.39089543493497664 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6905 0.29618489830198763 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5935 0.25457746146593724 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5727 0.2456554543918151 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4942 0.21198345653995987 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4812 0.20640720211863353 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4686 0.20100252475642488 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4680 0.2007451591677483 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4663 0.20001595666649793 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4271 0.18320140487296005 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4209 0.18054196045663518 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4176 0.1791264497189139 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4153 0.17813988162898692 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3796 0.1628266291027292 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3708 0.15905193380213906 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3509 0.15051597511103182 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3272 0.1403500343583061 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3139 0.13464509714264145 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.12400731947734196 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2819 0.12091893241322277 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2627 0.11268323357557154 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2565 0.11002378915924667 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2516 0.10792197018505445 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2489 0.10676382503600973 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2463 0.10564857415174447 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2462 0.10560567988696504 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2428 0.1041472748844643 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.2894264779433404E-5 0.0 0.0 0.0 0.0 8 8.578852955886681E-5 0.0 0.0 4.2894264779433404E-5 0.0 9 8.578852955886681E-5 0.0 0.0 4.2894264779433404E-5 0.0 10-11 8.578852955886681E-5 0.0 0.0 4.2894264779433404E-5 0.0 12-13 8.578852955886681E-5 0.0 0.0 1.286827943383002E-4 0.0 14-15 8.578852955886681E-5 0.0 0.0 1.501299267280169E-4 0.0 16-17 8.578852955886681E-5 0.0 0.0 2.7881272106631714E-4 0.0 18-19 8.578852955886681E-5 0.0 0.0 4.932840449634841E-4 0.0 20-21 8.578852955886681E-5 0.0 0.0 8.149910308092347E-4 0.0 22-23 1.286827943383002E-4 0.0 0.0 0.001372616472941869 0.0 24-25 1.286827943383002E-4 0.0 0.0 0.0024020788276482704 0.0 26-27 1.286827943383002E-4 0.0 0.0 0.004246532213163907 0.0 28-29 2.359184562868837E-4 0.0 0.0 0.011988947005851635 0.0 30-31 2.573655886766004E-4 0.0 0.0 0.0303047980666697 0.0 32-33 2.7881272106631714E-4 0.0 0.0 0.06288299216664936 0.0 34-35 3.002598534560338E-4 0.0 0.0 0.10993800062968781 0.0 36-37 3.002598534560338E-4 0.0 0.0 0.18073498464814264 0.0 38-39 3.002598534560338E-4 0.0 0.0 0.27930600511128056 0.0 40-41 3.002598534560338E-4 0.0 0.0 0.38585535882339317 0.0 42-43 3.002598534560338E-4 0.0 0.0 0.49783083703010406 0.0 44-45 3.002598534560338E-4 0.0 0.0 0.6342345990287023 0.0 46-47 3.002598534560338E-4 0.0 0.0 0.804975219983237 0.0 48-49 3.002598534560338E-4 0.0 0.0 0.9819140621983997 0.0 50-51 3.002598534560338E-4 0.0 0.0 1.169876730461877 0.0 52-53 3.002598534560338E-4 0.0 0.0 1.3470085968685472 0.0 54-55 3.002598534560338E-4 0.0 0.0 1.5467457408139786 0.0 56-57 3.002598534560338E-4 0.0 0.0 1.7884978171108652 0.0 58-59 3.002598534560338E-4 0.0 0.0 2.0593322049282077 0.0 60-61 3.002598534560338E-4 2.1447132389716702E-5 0.0 2.3730179632602044 0.0 62-63 3.002598534560338E-4 4.2894264779433404E-5 0.0 2.678532364151719 0.0 64-65 3.002598534560338E-4 4.2894264779433404E-5 0.0 2.986620420929999 0.0 66-67 3.002598534560338E-4 4.2894264779433404E-5 0.0 3.345902782722533 0.0 68-69 3.002598534560338E-4 4.2894264779433404E-5 0.0 3.7562936609997624 0.0 70-71 3.002598534560338E-4 4.2894264779433404E-5 0.0 4.205117800519364 0.0 72-73 3.002598534560338E-4 4.2894264779433404E-5 0.0 4.660547656814998 0.0 74-75 3.002598534560338E-4 4.2894264779433404E-5 0.0 5.144673776248073 0.0 76-77 3.002598534560338E-4 4.2894264779433404E-5 0.0 5.634226020175746 0.0 78-79 3.002598534560338E-4 4.2894264779433404E-5 0.0 6.214992918156885 0.0 80-81 3.002598534560338E-4 4.2894264779433404E-5 0.0 6.850299873805072 0.0 82-83 3.002598534560338E-4 4.2894264779433404E-5 0.0 7.4738966951684755 0.0 84-85 3.002598534560338E-4 4.2894264779433404E-5 0.0 8.149138211326317 0.0 86-87 3.002598534560338E-4 4.2894264779433404E-5 0.0 8.852625600841414 0.0 88-89 3.002598534560338E-4 4.2894264779433404E-5 0.0 9.589120127104286 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 800 0.0 26.100134 3 GTAAGCG 330 0.0 25.167038 94-95 GAGTACT 11585 0.0 22.921339 12-13 GTACATG 10900 0.0 22.384306 1 CTTTGCG 985 0.0 21.679853 2 GTACTTT 12425 0.0 21.35263 14-15 AGTACTT 11940 0.0 21.206354 12-13 TACATGG 11175 0.0 21.02024 2 TTGCGTT 1000 0.0 20.880108 4 ACATGGG 11225 0.0 20.08109 3 CTATACG 120 0.0047912607 19.77283 4 CATGGGG 4145 0.0 19.46276 4 GTACGTA 150 7.557872E-4 19.061636 1 ACTTTTT 14165 0.0 18.72791 16-17 TACTTTT 13475 0.0 18.649755 14-15 GTGTAGG 1390 0.0 18.5131 1 GAGTGCA 1685 0.0 18.30718 9 CATGGGA 6915 0.0 17.293718 4 GTATAGG 450 7.2759576E-12 16.943678 1 AGAGTGC 1870 0.0 16.49498 8 >>END_MODULE