##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939024_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2341425 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94118709760082 33.0 31.0 34.0 30.0 34.0 2 31.994793768751936 33.0 31.0 34.0 30.0 34.0 3 32.03107979115283 34.0 31.0 34.0 30.0 34.0 4 35.5708489488239 37.0 35.0 37.0 33.0 37.0 5 35.60921789081436 37.0 35.0 37.0 33.0 37.0 6 35.70464866480882 37.0 35.0 37.0 33.0 37.0 7 35.631402671450076 37.0 35.0 37.0 33.0 37.0 8 35.627338479771936 37.0 35.0 37.0 33.0 37.0 9 37.242211900872334 39.0 37.0 39.0 34.0 39.0 10-11 37.134312865028775 39.0 37.0 39.0 33.0 39.0 12-13 37.022343658242306 39.0 37.0 39.0 33.0 39.0 14-15 38.220633161429475 40.0 38.0 41.0 33.0 41.0 16-17 38.16125970295867 40.0 38.0 41.0 33.0 41.0 18-19 38.13529474572109 40.0 38.0 41.0 33.0 41.0 20-21 38.030254011979885 40.0 38.0 41.0 33.0 41.0 22-23 38.09000309640497 40.0 38.0 41.0 33.0 41.0 24-25 38.00257151093885 40.0 38.0 41.0 33.0 41.0 26-27 37.89690551693947 40.0 38.0 41.0 33.0 41.0 28-29 37.766603030205964 40.0 37.5 41.0 33.0 41.0 30-31 37.56333130465422 40.0 37.0 41.0 32.5 41.0 32-33 37.344896804296525 40.0 36.5 41.0 32.0 41.0 34-35 36.99987998761438 40.0 36.0 41.0 31.0 41.0 36-37 36.702168124112454 39.0 35.5 41.0 30.0 41.0 38-39 36.47334956276626 39.0 35.0 41.0 30.0 41.0 40-41 36.30511483391524 39.0 35.0 41.0 30.0 41.0 42-43 36.07160212263899 39.0 35.0 41.0 30.0 41.0 44-45 35.955434831352704 39.0 35.0 41.0 29.0 41.0 46-47 35.646995312683515 39.0 35.0 41.0 27.0 41.0 48-49 35.38942631090042 39.0 35.0 41.0 26.0 41.0 50-51 34.1596538432792 38.0 34.0 40.0 24.5 40.5 52-53 34.285896409237964 38.0 33.5 39.5 24.0 40.5 54-55 34.76277822266355 38.0 35.0 40.0 24.5 41.0 56-57 34.63862242010741 38.0 34.5 40.0 24.5 41.0 58-59 34.430771859017476 38.0 34.0 40.0 24.0 41.0 60-61 34.12105790277288 37.5 34.0 40.0 23.0 41.0 62-63 33.747800377974954 37.0 34.0 40.0 22.5 41.0 64-65 33.31453452491539 36.5 33.0 39.5 21.5 41.0 66-67 32.907922312267104 36.0 33.0 39.0 20.0 41.0 68-69 32.50118346733293 35.0 32.5 39.0 19.0 40.0 70-71 32.04694149930064 35.0 32.0 37.5 17.5 40.0 72-73 31.605270081253938 35.0 31.5 37.0 14.0 39.5 74-75 31.153768538390082 35.0 31.0 37.0 9.5 39.0 76-77 30.739587644276455 35.0 31.0 36.0 7.0 39.0 78-79 30.315210395378884 34.5 30.5 36.0 4.0 37.5 80-81 29.885442412206242 34.0 30.0 35.0 2.0 37.0 82-83 29.53819404849611 34.0 30.0 35.0 2.0 37.0 84-85 29.210794067715174 34.0 29.5 35.0 2.0 36.0 86-87 28.828314381199483 34.0 29.0 35.0 2.0 36.0 88-89 28.39762772670489 34.0 29.0 35.0 2.0 35.5 90-91 27.966210107092905 34.0 28.0 35.0 2.0 35.0 92-93 27.547733538336697 34.0 27.0 35.0 2.0 35.0 94-95 27.075902068184973 33.5 26.0 35.0 2.0 35.0 96-97 26.47549099373245 33.0 25.0 35.0 2.0 35.0 98-99 25.771278174615887 33.0 23.5 35.0 2.0 35.0 100-101 23.265029842937526 29.5 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.251948440019099 1101 2 0.3641961014892843 1101 3 0.40362018022956647 1101 4 0.34287189146116503 1101 5 0.18941451245495244 1101 6 0.31040144220290244 1101 7 0.3107273202392591 1101 8 0.34405790474429665 1101 9 0.29723246243857915 1101 10-11 0.5194646142601798 1101 12-13 0.5117929185817758 1101 14-15 0.2736424254604586 1101 16-17 0.5590487690833541 1101 18-19 0.6156682860148379 1101 20-21 0.6711096753826027 1101 22-23 0.6162599937786126 1101 24-25 0.6509067523309113 1101 26-27 0.5818025837285532 1101 28-29 0.4643140639127168 1101 30-31 0.5758875059523447 1101 32-33 0.5811114194074207 1101 34-35 0.4247188708170029 1101 36-37 0.3333203841956447 1101 38-39 0.3810194371415534 1101 40-41 0.2718877351425135 1101 42-43 0.10526646457484645 1101 44-45 0.1768556282032563 1101 46-47 0.23601940279908007 1101 48-49 0.24647969206512244 1101 50-51 0.2236672484436184 1101 52-53 0.2974003045869722 1101 54-55 0.21541560165874785 1101 56-57 0.16977510377665794 1101 58-59 0.24183826224502525 1101 60-61 0.2739479221171308 1101 62-63 0.2503494868931355 1101 64-65 0.1605042929332825 1101 66-67 0.182260008338055 1101 68-69 0.11433936647184595 1101 70-71 0.15312395470402862 1101 72-73 -0.08954637975865154 1101 74-75 -0.26862978696924955 1101 76-77 -0.156638528401718 1101 78-79 -0.1173102302776563 1101 80-81 -0.1500812741027815 1101 82-83 -0.14018617524722998 1101 84-85 -0.42619800011729225 1101 86-87 -0.30043878923837397 1101 88-89 -0.4292537881105041 1101 90-91 -0.44399521318240076 1101 92-93 -0.4094915315365206 1101 94-95 -0.5088435018239821 1101 96-97 -0.7503769377765117 1101 98-99 -0.8701813547555872 1101 100-101 -0.4173157673958272 1102 1 0.2388946323375265 1102 2 0.19493635793050856 1102 3 0.18980242090452037 1102 4 0.22791184848497892 1102 5 0.312204174850379 1102 6 0.2583894321917839 1102 7 0.2845362094798034 1102 8 0.21102912485982017 1102 9 0.33284751742893803 1102 10-11 0.17789535888919517 1102 12-13 0.2644589411609317 1102 14-15 0.3836199171005106 1102 16-17 0.2817491059799906 1102 18-19 0.3802561394127437 1102 20-21 0.355591191875547 1102 22-23 0.3391371077113092 1102 24-25 0.4160996870795657 1102 26-27 0.4346172739210701 1102 28-29 0.32181667704794137 1102 30-31 0.2868711067069327 1102 32-33 0.3506226902448262 1102 34-35 0.3514236552489649 1102 36-37 0.20711834199402546 1102 38-39 0.11532094922028335 1102 40-41 0.09213599339939549 1102 42-43 0.06594096578876218 1102 44-45 0.14198901261939056 1102 46-47 0.34589455531872204 1102 48-49 0.5516445382128836 1102 50-51 0.45030332095343795 1102 52-53 0.25341301421038054 1102 54-55 0.29220242396138474 1102 56-57 0.25223492812400394 1102 58-59 0.2530293521826863 1102 60-61 0.2465216625026585 1102 62-63 0.09710201570009502 1102 64-65 0.1829407135835055 1102 66-67 0.10565277401374118 1102 68-69 0.1072857753369405 1102 70-71 0.20458616522902418 1102 72-73 0.27617688609818813 1102 74-75 0.12616216165921657 1102 76-77 -0.050264560642187206 1102 78-79 0.0801283766609977 1102 80-81 -0.10175857376588482 1102 82-83 -0.06056300808569759 1102 84-85 -0.07886579254898507 1102 86-87 -0.15066106189561168 1102 88-89 -0.29378490211815844 1102 90-91 -0.2695031608613938 1102 92-93 -0.11855171901596151 1102 94-95 -0.2922011619539937 1102 96-97 -0.5732298984274813 1102 98-99 -0.7104722560477867 1102 100-101 -0.22142442801306927 1103 1 -0.018488998337247153 1103 2 0.11463693445089973 1103 3 0.09979721303407985 1103 4 0.17860859842497234 1103 5 0.09406936492934648 1103 6 0.12041076701797948 1103 7 0.06641219737628035 1103 8 0.06693723377158989 1103 9 0.18302759180097894 1103 10-11 0.1859877001633521 1103 12-13 0.2233850815973497 1103 14-15 0.19976142914381256 1103 16-17 0.24282172794757173 1103 18-19 0.21396715410027412 1103 20-21 0.30949655481926897 1103 22-23 0.2891729066293678 1103 24-25 0.3669155244876947 1103 26-27 0.2658259982253881 1103 28-29 0.24261001346557265 1103 30-31 0.27490141451948347 1103 32-33 0.18641135037687917 1103 34-35 0.2090519395505197 1103 36-37 0.2975107139173758 1103 38-39 0.3815769596592773 1103 40-41 0.31568476672579493 1103 42-43 0.20669861541951917 1103 44-45 0.3045072754665128 1103 46-47 0.16355617165233127 1103 48-49 0.028383755138541744 1103 50-51 0.0108238422056246 1103 52-53 0.10673795293276811 1103 54-55 0.2126858422513891 1103 56-57 0.18950428051243762 1103 58-59 0.07656165883738453 1103 60-61 0.01971620511957184 1103 62-63 -0.011198328916414368 1103 64-65 -0.15075327377640235 1103 66-67 -0.038438390298317415 1103 68-69 -0.12801154256825242 1103 70-71 -0.1557678412181005 1103 72-73 -0.009434419881230838 1103 74-75 -0.007114157813319366 1103 76-77 -0.07605680897205147 1103 78-79 -0.00521663852655152 1103 80-81 -0.06920118128930142 1103 82-83 -0.0074408606821982914 1103 84-85 -0.12846172575867243 1103 86-87 0.01751525732774084 1103 88-89 0.0655579510140818 1103 90-91 -0.18422573585846536 1103 92-93 -0.194554026426502 1103 94-95 -0.18396394303987407 1103 96-97 -0.2271463498991828 1103 98-99 -0.13711593305350078 1103 100-101 -0.04032229062662296 1104 1 0.060604476465570656 1104 2 0.11727127233211121 1104 3 0.08380241257580678 1104 4 0.03489922631310094 1104 5 0.12563319127044537 1104 6 0.19787296839879787 1104 7 0.1362398487130605 1104 8 0.09804423731832657 1104 9 0.1710365626663659 1104 10-11 0.1392823819139366 1104 12-13 0.22204348805785656 1104 14-15 0.30371076259029195 1104 16-17 0.04014216316080166 1104 18-19 0.14209197621984515 1104 20-21 0.16814839975618412 1104 22-23 0.24815065437086758 1104 24-25 0.2383782785268096 1104 26-27 0.2790690985349329 1104 28-29 0.2681865012703497 1104 30-31 0.4519695105081567 1104 32-33 0.390906408017905 1104 34-35 0.4030787797008344 1104 36-37 0.4506324570885738 1104 38-39 0.4678075920390512 1104 40-41 0.5283797624773712 1104 42-43 0.5792983552354443 1104 44-45 0.7171745348091818 1104 46-47 0.5972950292227637 1104 48-49 0.6125389175776519 1104 50-51 0.5043050616554652 1104 52-53 0.5411360676849171 1104 54-55 0.6240716381052138 1104 56-57 0.6294561971707395 1104 58-59 0.4894464627006059 1104 60-61 0.45891375355220987 1104 62-63 0.5539941338179659 1104 64-65 0.5926227439583442 1104 66-67 0.6695037441011493 1104 68-69 0.6300569063351773 1104 70-71 0.5665635902393262 1104 72-73 0.47605726488827216 1104 74-75 0.39014014469362124 1104 76-77 0.46682388617686854 1104 78-79 0.4576328221141388 1104 80-81 0.10937202885849473 1104 82-83 0.08122612543614238 1104 84-85 0.3202712481742793 1104 86-87 0.2956887734039917 1104 88-89 0.3896528178120455 1104 90-91 0.25258793032557136 1104 92-93 0.3253604046821579 1104 94-95 0.09594009726485453 1104 96-97 -0.005957803380152171 1104 98-99 0.13801910082528224 1104 100-101 0.5571717041303685 1105 1 0.12992636030566018 1105 2 0.15336258773291433 1105 3 0.14756929991762746 1105 4 0.21101101337529826 1105 5 0.225461306566352 1105 6 0.2018341433949118 1105 7 0.09609628280173155 1105 8 0.15728418368949093 1105 9 0.21030799920981025 1105 10-11 0.09151551083559895 1105 12-13 0.35909770340062863 1105 14-15 0.33997030341113543 1105 16-17 0.3046442616585665 1105 18-19 0.2740326470276031 1105 20-21 0.2936827822875685 1105 22-23 0.2501838949006867 1105 24-25 0.12339107077400513 1105 26-27 0.14283446117506315 1105 28-29 0.3932410093558403 1105 30-31 0.31397780691829524 1105 32-33 0.18509749786450413 1105 34-35 0.31580810754117294 1105 36-37 0.3750736285067262 1105 38-39 0.43750068991778335 1105 40-41 0.3450153760529133 1105 42-43 0.2754731836302611 1105 44-45 0.10012044144254872 1105 46-47 0.24936074589863466 1105 48-49 0.31904095712027924 1105 50-51 0.19469628994498578 1105 52-53 0.16756240070619555 1105 54-55 0.18102788628192457 1105 56-57 0.14303854478443156 1105 58-59 0.11342869890943064 1105 60-61 0.16098165563383304 1105 62-63 0.1605331727883268 1105 64-65 0.16470006869641907 1105 66-67 -0.02068734167958297 1105 68-69 -0.008378180506753097 1105 70-71 -0.1447385151704097 1105 72-73 -0.004017933399179441 1105 74-75 -0.10996465313601789 1105 76-77 -0.035221247026154145 1105 78-79 -0.04204414442068938 1105 80-81 0.05189595905155642 1105 82-83 -0.0013395205952129174 1105 84-85 0.08277171829063334 1105 86-87 0.08113010847345592 1105 88-89 0.050049301240061794 1105 90-91 0.020129470823174955 1105 92-93 0.22456786474259616 1105 94-95 0.14583118274064688 1105 96-97 -0.09062652127883197 1105 98-99 -0.16908499259217535 1105 100-101 0.07659182958282429 1106 1 0.06574591796810836 1106 2 0.07136950546025389 1106 3 0.15875902867113467 1106 4 0.0940852445283582 1106 5 0.1416864327961207 1106 6 0.09782968422724281 1106 7 0.051923678783794 1106 8 0.14215399959447694 1106 9 0.18212169884196072 1106 10-11 0.20419467661331936 1106 12-13 0.1454456638354813 1106 14-15 0.06494609683586106 1106 16-17 0.07547471325674593 1106 18-19 0.14505145665021502 1106 20-21 0.1297690030218419 1106 22-23 0.1707504772448516 1106 24-25 0.13698690185078988 1106 26-27 0.17091252552430802 1106 28-29 0.20038493825298076 1106 30-31 0.23267643876813082 1106 32-33 0.2710996110067043 1106 34-35 0.12183069956328296 1106 36-37 0.039229571211180314 1106 38-39 0.0705051733115667 1106 40-41 0.213025849548373 1106 42-43 0.24755649644955469 1106 44-45 0.20010092379225597 1106 46-47 -0.05008318667150036 1106 48-49 0.006871184431545885 1106 50-51 -0.053965705770906425 1106 52-53 -0.21271771958036112 1106 54-55 -0.24107373288202893 1106 56-57 -0.14624732021673026 1106 58-59 0.030194321430641935 1106 60-61 0.0024799703560347552 1106 62-63 -0.048675621915691636 1106 64-65 -0.275421968391413 1106 66-67 -0.12913729248494832 1106 68-69 -0.17793859769603415 1106 70-71 -0.1307351383629296 1106 72-73 -0.1355510712043433 1106 74-75 -0.1620889901415623 1106 76-77 -0.2282181812506927 1106 78-79 4.5842081202707163E-4 1106 80-81 0.05263475779585747 1106 82-83 0.03580965989792162 1106 84-85 0.16882980547493887 1106 86-87 0.14692696001661787 1106 88-89 0.2538443412952027 1106 90-91 0.24200764317399148 1106 92-93 0.1856553553940543 1106 94-95 0.13672216445305807 1106 96-97 0.23569403629687002 1106 98-99 0.3176800281836698 1106 100-101 0.6423657249307055 1107 1 -0.017532371198594632 1107 2 -0.03305937848643836 1107 3 0.054602083107290866 1107 4 0.04265631816230098 1107 5 0.06672158026398733 1107 6 0.06892013441783007 1107 7 0.010604951849316535 1107 8 0.09237942818148781 1107 9 0.15466501203619742 1107 10-11 0.12276806816216634 1107 12-13 0.07685772850681616 1107 14-15 0.05902935391534214 1107 16-17 0.1967838503864101 1107 18-19 0.1947938348006062 1107 20-21 0.1912202601498123 1107 22-23 0.03465781518775657 1107 24-25 0.22756700171807864 1107 26-27 0.16060575781877873 1107 28-29 0.1828793782871614 1107 30-31 0.028343149874125118 1107 32-33 0.0855103758277238 1107 34-35 0.07574964223049108 1107 36-37 0.07848338440845737 1107 38-39 0.08654650794576213 1107 40-41 0.06567955912120738 1107 42-43 0.09063289977280675 1107 44-45 0.08959130065795051 1107 46-47 0.10708962373215059 1107 48-49 0.2096853300753878 1107 50-51 0.10098879946788486 1107 52-53 -0.028132914194316072 1107 54-55 0.05509557186469749 1107 56-57 0.22990330486280186 1107 58-59 0.14718463769725787 1107 60-61 0.06720130547502379 1107 62-63 0.02943621672107355 1107 64-65 0.19553061888642276 1107 66-67 0.14754334732017327 1107 68-69 0.0778645219447256 1107 70-71 0.14069686340656062 1107 72-73 0.06686160551773312 1107 74-75 0.003480959379402293 1107 76-77 0.1076465525072372 1107 78-79 0.18848601157430167 1107 80-81 0.07378686755291852 1107 82-83 0.15181751353901873 1107 84-85 0.25100443385106885 1107 86-87 0.252514801416698 1107 88-89 0.42566508617912646 1107 90-91 0.09068302297874098 1107 92-93 0.12706134500736965 1107 94-95 0.11503981272386454 1107 96-97 0.12604832883288353 1107 98-99 0.1459524543891355 1107 100-101 0.2735888719622608 1108 1 0.1385279396944128 1108 2 0.19112280345430577 1108 3 0.13710863090855696 1108 4 0.21073226956478663 1108 5 0.12561120613484178 1108 6 0.17625730521108096 1108 7 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-1.5993913745376958 2316 70-71 -1.4139408062836907 2316 72-73 -1.4582935004412718 2316 74-75 -1.5492640871144836 2316 76-77 -1.3438613454499908 2316 78-79 -1.1795580978861473 2316 80-81 -1.1848259021049543 2316 82-83 -1.2631290551793946 2316 84-85 -1.3962329790828072 2316 86-87 -1.43629043243536 2316 88-89 -1.577573703924653 2316 90-91 -1.7850850782247498 2316 92-93 -1.8950061199448385 2316 94-95 -1.8738148743232195 2316 96-97 -2.1400319945564483 2316 98-99 -2.486967106396147 2316 100-101 -2.8657432309537505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7412.0 3 1563.0 4 1541.0 5 1630.0 6 2040.0 7 2678.0 8 3148.0 9 4080.0 10 5101.0 11 6923.0 12 9563.0 13 14792.0 14 14495.0 15 13977.0 16 17992.0 17 24942.0 18 21344.0 19 14503.0 20 14634.0 21 15330.0 22 16461.0 23 18003.0 24 20360.0 25 23321.0 26 27406.0 27 31884.0 28 37579.0 29 45249.0 30 54797.0 31 67353.0 32 83818.0 33 108297.0 34 149561.0 35 223316.0 36 329112.0 37 472087.0 38 379972.0 39 55151.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.451045156338736 18.2794644317918 14.156872385824283 22.112618026045176 2 15.821693155844937 21.28172257385815 38.43047504406076 24.46610922623615 3 20.434984452627322 24.695315098264352 30.498670116128658 24.371030332979668 4 12.927843461174401 16.41724362312409 36.95458482649408 33.70032808920743 5 13.638023280571677 37.56076454950221 33.6404089705363 15.160803199389811 6 30.996273054039648 35.44518388731706 17.1890475064855 16.369495552157794 7 27.58220322644217 33.9459985388373 20.70191383243995 17.769884402280574 8 26.033089116960962 32.624280827027015 19.944223083726502 21.398406972285525 9 26.257467814920027 17.396978888126522 21.30882073182983 35.03673256512362 10-11 25.271416843479017 25.415388798015854 26.869407220697934 22.443787137807195 12-13 27.69012226458585 23.491741657978064 27.040774668689522 21.77736140874657 14-15 23.729312089915787 24.74932959681755 27.36268892330417 24.158669389962494 16-17 21.834190310758245 28.095671980803022 27.704619256867307 22.365518451571425 18-19 21.439205976551836 27.25261636600288 29.71863444281061 21.589543214634677 20-21 22.848642994883132 25.599741260210703 29.105832141065342 22.445783603840823 22-23 22.24798155418991 25.565704646834785 29.29877915161673 22.88753464735857 24-25 22.964575904225498 25.68310018460607 29.372613046094216 21.979710865074217 26-27 22.834269043685275 25.148374789040208 30.38007081278616 21.637285354488363 28-29 21.571798880350023 26.40852086814779 30.256633489952055 21.763046761550132 30-31 21.956563706770066 26.03438961312166 29.7098011615992 22.29924551850907 32-33 21.961979881711958 26.25801296097641 29.64165982931052 22.13834732800112 34-35 22.945917923102826 25.570227344975354 29.179850520233913 22.304004211687907 36-37 22.998498373040565 25.314926034508424 29.80498610603063 21.88158948642038 38-39 22.710259199326526 25.638323574478868 29.759205232306552 21.892211993888054 40-41 22.284595630965626 26.047101142815187 29.812590674487232 21.855712551731955 42-43 21.913113725123036 26.55000602336033 28.67881832192402 22.85806192959261 44-45 22.2349373265634 26.273787125097726 28.504128113267523 22.98714743507135 46-47 22.802384772538822 26.63237963764532 27.96702242967011 22.598213160145754 48-49 23.072782301805002 26.34026365138044 28.053589495187296 22.53336455162725 50-51 22.273759626301675 26.830678041314215 28.534061439077064 22.36150089330705 52-53 22.511844320192832 27.325781579072288 27.8418474755279 22.32052662520698 54-55 21.80873215254882 26.394006774401603 28.310542590006953 23.486718483042626 56-57 22.665928051591404 26.968060265837014 27.488762422528616 22.877249260042966 58-59 23.304942006074747 26.32048012267149 27.5634263939937 22.811151477260065 60-61 22.45288031367917 26.277626324879055 28.776055525608317 22.49343783583345 62-63 22.663707397421483 26.2077222324229 28.527999776658923 22.600570593496695 64-65 22.20369735450281 26.937441944195967 28.43756954881242 22.421291152488806 66-67 22.146523551059758 26.481844457922676 28.04747488354615 23.324157107471414 68-69 21.515051185439173 26.914665438854957 28.43189093388101 23.138392441824866 70-71 22.387308707860946 26.808335105800158 27.650009775423083 23.154346410915817 72-73 22.190088743254673 26.1216755114891 28.208470706820293 23.479765038435932 74-75 21.656385640003307 27.507301265409456 27.61017955903472 23.226133535552517 76-77 22.282627606284336 26.36326653873928 27.69101797641892 23.66308787855747 78-79 22.070472671659864 26.37572583639351 28.222589039975144 23.331212451971485 80-81 22.174355005780427 27.154067685098184 27.790172244677798 22.881405064443594 82-83 21.91338776127301 26.701281152607837 28.07321142024623 23.312119665872927 84-85 21.894015233342305 26.442540127505087 27.666989334770342 23.996455304382266 86-87 22.089701736560986 26.4926648064261 27.92070332642025 23.496930130592666 88-89 22.194887259387176 26.179491475185895 27.698708283395835 23.926912982031094 90-91 21.977356528874665 25.866551526731907 28.20217369589524 23.953918248498187 92-93 22.039055330031246 26.400050528139364 28.04771471881262 23.51317942301677 94-95 21.940854303807747 26.158236585362708 27.877959422352205 24.022949688477336 96-97 21.214936025075687 25.917572551136654 28.18973982320618 24.67775160058148 98-99 21.873588151244274 26.06813911056563 27.461066541834978 24.597206196355117 100-101 22.006173741067148 25.173115617176506 28.303630187239794 24.517080454516556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7939.0 1 4893.5 2 2153.0 3 2312.0 4 1826.5 5 1829.0 6 3085.0 7 5740.0 8 8435.0 9 9088.5 10 7173.0 11 5337.0 12 5165.0 13 5246.0 14 5466.0 15 5796.5 16 6206.5 17 6377.5 18 6331.0 19 6567.0 20 6854.5 21 7532.5 22 8593.5 23 9585.0 24 10888.5 25 12489.5 26 14569.5 27 16401.0 28 17659.0 29 19045.5 30 20868.5 31 23079.0 32 25640.5 33 28635.5 34 31693.5 35 34165.0 36 37782.0 37 42789.0 38 45863.5 39 46771.5 40 48948.5 41 53589.0 42 57418.5 43 61400.0 44 64909.0 45 67908.0 46 73999.0 47 92617.0 48 146366.0 49 149795.0 50 100325.5 51 82250.0 52 77353.0 53 77269.0 54 77885.5 55 74478.0 56 67417.5 57 60845.5 58 56164.5 59 51365.5 60 45758.0 61 39633.0 62 34056.5 63 29057.0 64 23791.5 65 18612.0 66 13124.0 67 10123.5 68 7175.0 69 3855.0 70 2761.5 71 2098.0 72 1534.5 73 1084.0 74 819.0 75 613.0 76 411.5 77 248.0 78 133.0 79 80.5 80 51.0 81 40.0 82 29.0 83 25.5 84 20.0 85 16.5 86 15.0 87 11.0 88 8.0 89 6.0 90 4.5 91 5.5 92 5.0 93 2.0 94 1.0 95 1.0 96 1.0 97 1.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28012855419242555 2 0.0872118474860395 3 0.09067127924234174 4 0.10365482558698229 5 0.10587569535646028 6 0.08439305124016358 7 0.09361820259030292 8 0.08994522566385856 9 0.09481405554309875 10-11 0.08885614529613377 12-13 0.09199525929722284 14-15 0.10025945738172266 16-17 0.08962490790864537 18-19 0.10038758448380795 20-21 0.10230949101508696 22-23 0.11456698378124433 24-25 0.12522288777133583 26-27 0.12836200177242493 28-29 0.12116552953863564 30-31 0.11388363923678956 32-33 0.10924970904470567 34-35 0.37876506828106815 36-37 0.5815475618480199 38-39 0.5636524765901106 40-41 0.5275419883190792 42-43 0.5548117065462271 44-45 0.5759313238732823 46-47 0.5653181289172192 48-49 0.5863950372102459 50-51 0.5790490833573572 52-53 0.5817611070181621 54-55 0.5721515743617669 56-57 0.5442198661071783 58-59 0.5382833103772275 60-61 0.48692569695804905 62-63 0.5615170248886896 64-65 0.5946378807777315 66-67 0.6071302732310452 68-69 0.6085823803880116 70-71 0.6047599218424677 72-73 0.5970936502343659 74-75 0.5944029810905752 76-77 0.5783657388129024 78-79 0.5548117065462271 80-81 0.5499642311840012 82-83 0.5641436304814376 84-85 0.573988062824989 86-87 0.5651045837470771 88-89 0.5772125948941349 90-91 0.5903456228578751 92-93 0.5983108577041759 94-95 0.5973285499215222 96-97 0.5941467268864046 98-99 0.5989728477316164 100-101 0.5766146684177371 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2341425.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.2509655166078 #Duplication Level Percentage of deduplicated Percentage of total 1 65.25125984535477 23.001699107238164 2 15.14511339184298 10.677597398419438 3 6.648494828345526 7.030975857940604 4 3.6827070229480228 5.192759130948406 5 2.194549683477885 3.867999760838074 6 1.5087878125691425 3.1911736291651773 7 1.0140639951523651 2.5022714447324694 8 0.7770763372597284 2.1914152934811666 9 0.5641884305988542 1.7899368220718352 >10 2.9098309858495335 17.948594029878056 >50 0.18073495827894545 4.377379396276155 >100 0.10981855692689403 7.1153946418077165 >500 0.005957576530613241 1.4311989341814193 >1k 0.00607915972511555 4.937814188813262 >5k 8.51082361516177E-4 2.252553600568168 >10k+ 4.86332778009244E-4 2.491236763639959 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17562 0.7500560556071623 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15820 0.6756569183296497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13431 0.5736250360357474 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11313 0.4831673019635478 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9812 0.4190610418868851 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9079 0.38775531994405116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8782 0.3750707368376096 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7348 0.3138259820408512 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6436 0.2748753430069295 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6031 0.2575781842254183 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5069 0.21649209349007548 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4708 0.20107413220581483 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4646 0.19842617209605262 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4337 0.18522908058126994 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4222 0.18031754166800132 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4118 0.17587580212904533 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4079 0.17421014980193686 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3967 0.16942673799075347 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3832 0.1636610183969164 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3807 0.16259329254620583 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3674 0.1569129910204256 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3539 0.15114727142658851 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3017 0.12885315566375177 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2917 0.12458225226090948 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2899 0.12381348964839788 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2803 0.1197134223816693 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.11326435824337744 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2583 0.11031743489541626 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2481 0.10596111342451713 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2472 0.10557673211826131 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2417 0.10322773524669805 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.270903402842286E-5 0.0 8 0.0 0.0 0.0 4.270903402842286E-5 0.0 9 0.0 0.0 0.0 4.270903402842286E-5 0.0 10-11 0.0 0.0 0.0 4.270903402842286E-5 0.0 12-13 0.0 0.0 0.0 4.270903402842286E-5 0.0 14-15 0.0 0.0 0.0 8.541806805684572E-5 0.0 16-17 2.135451701421143E-5 0.0 0.0 1.9219065312790288E-4 0.0 18-19 4.270903402842286E-5 0.0 0.0 2.9896323819896003E-4 0.0 20-21 4.270903402842286E-5 0.0 0.0 5.765719593837087E-4 0.0 22-23 4.270903402842286E-5 0.0 0.0 0.0011531439187674173 0.0 24-25 4.270903402842286E-5 0.0 0.0 0.001985970082321663 0.0 26-27 4.270903402842286E-5 0.0 0.0 0.0038438130625580574 0.0 28-29 4.270903402842286E-5 0.0 0.0 0.01148873015364575 0.0 30-31 4.270903402842286E-5 0.0 0.0 0.030643731915393404 0.0 32-33 4.270903402842286E-5 0.0 0.0 0.06241925323254001 0.0 34-35 4.270903402842286E-5 0.0 0.0 0.11565606414896912 0.0 36-37 4.270903402842286E-5 0.0 0.0 0.19708083752415728 0.0 38-39 4.270903402842286E-5 0.0 0.0 0.30129088055350906 0.0 40-41 4.270903402842286E-5 0.0 0.0 0.4148542020350855 0.0 42-43 4.270903402842286E-5 0.0 0.0 0.5308946474903105 0.0 44-45 4.270903402842286E-5 0.0 0.0 0.6712792423417364 0.0 46-47 1.2812710208526858E-4 0.0 0.0 0.8454893921436732 0.0 48-49 1.2812710208526858E-4 0.0 0.0 1.0240131543824809 0.0 50-51 1.2812710208526858E-4 0.0 0.0 1.213619910951664 0.0 52-53 1.2812710208526858E-4 0.0 0.0 1.392165027707486 0.0 54-55 1.2812710208526858E-4 0.0 0.0 1.593153741845244 0.0 56-57 1.2812710208526858E-4 0.0 0.0 1.8429161728434607 0.0 58-59 1.2812710208526858E-4 0.0 0.0 2.1123461140117663 0.0 60-61 1.2812710208526858E-4 0.0 0.0 2.432706578258966 0.0 62-63 1.2812710208526858E-4 0.0 0.0 2.743265319196643 0.0 64-65 1.2812710208526858E-4 0.0 0.0 3.0488484576700086 0.0 66-67 1.2812710208526858E-4 0.0 0.0 3.406344427004922 0.0 68-69 1.2812710208526858E-4 0.0 0.0 3.8161803175416678 0.0 70-71 1.2812710208526858E-4 0.0 0.0 4.267166362364799 0.0 72-73 1.4948161909948002E-4 0.0 0.0 4.720095668236223 0.0 74-75 1.7083613611369145E-4 0.0 0.0 5.205868221275505 0.0 76-77 1.7083613611369145E-4 0.0 0.0 5.702446159923978 0.0 78-79 1.7083613611369145E-4 4.270903402842286E-5 0.0 6.284997384071666 0.0 80-81 1.7083613611369145E-4 4.270903402842286E-5 0.0 6.9187567400194325 0.0 82-83 1.7083613611369145E-4 4.270903402842286E-5 0.0 7.542564891038577 0.0 84-85 1.9219065312790288E-4 4.270903402842286E-5 0.0 8.21909734456581 0.0 86-87 2.135451701421143E-4 4.270903402842286E-5 0.0 8.94045292930587 4.270903402842286E-5 88-89 2.135451701421143E-4 4.270903402842286E-5 0.0 9.676585839819769 4.270903402842286E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 805 0.0 26.462358 3 GTACATG 11130 0.0 24.202202 1 TAACACG 100 0.0016873297 23.66911 5 GAGTACT 10720 0.0 23.184105 12-13 TACATGG 11370 0.0 22.85724 2 ACATGGG 11415 0.0 22.062138 3 CTTTGCG 980 0.0 21.736935 2 CATGGGG 4270 0.0 21.618156 4 GTACTTT 11875 0.0 20.88995 14-15 AGTACTT 11240 0.0 20.76378 12-13 TTGCGTT 1075 0.0 20.256353 4 ACTTTTT 13605 0.0 18.461164 16-17 TACTTTT 12610 0.0 18.320803 14-15 CATGGGA 6885 0.0 17.876451 4 GAGTGCA 1770 0.0 17.652292 9 GCTTATA 1250 0.0 17.452173 1 AGAGTGC 1950 0.0 17.235966 8 ATGGGGA 2480 0.0 16.988312 5 ACTTGAC 1180 0.0 16.849195 8 GCTTTGC 1530 0.0 16.428057 1 >>END_MODULE