##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939024_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2341425 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11283769499343 33.0 31.0 34.0 30.0 34.0 2 32.329934548405355 34.0 31.0 34.0 30.0 34.0 3 32.41304718280534 34.0 31.0 34.0 30.0 34.0 4 35.99896387883447 37.0 35.0 37.0 35.0 37.0 5 35.91625954279979 37.0 35.0 37.0 35.0 37.0 6 35.972299774709846 37.0 35.0 37.0 35.0 37.0 7 35.914194988094856 37.0 35.0 37.0 35.0 37.0 8 35.89571265361906 37.0 35.0 37.0 35.0 37.0 9 37.54291724056931 39.0 37.0 39.0 35.0 39.0 10-11 37.46606959437095 39.0 37.0 39.0 34.0 39.0 12-13 37.39150218349936 39.0 37.0 39.0 34.0 39.0 14-15 38.58200177242492 40.0 38.0 41.0 34.0 41.0 16-17 38.58170772072563 40.0 38.0 41.0 34.0 41.0 18-19 38.7051981165316 40.0 38.0 41.0 34.0 41.0 20-21 38.70589149769905 40.0 38.0 41.0 34.0 41.0 22-23 38.644877585231214 40.0 38.0 41.0 34.0 41.0 24-25 38.55695206978656 40.0 38.0 41.0 34.0 41.0 26-27 38.365746927618865 40.0 38.0 41.0 34.0 41.0 28-29 38.16934964818434 40.0 38.0 41.0 33.0 41.0 30-31 37.977455609297756 40.0 37.0 41.0 33.0 41.0 32-33 37.77712546846472 40.0 37.0 41.0 33.0 41.0 34-35 37.71432097974524 40.0 37.0 41.0 33.0 41.0 36-37 37.56530916002008 40.0 36.5 41.0 33.0 41.0 38-39 37.40830669357336 40.0 36.0 41.0 32.0 41.0 40-41 37.22812774272078 40.0 36.0 41.0 32.0 41.0 42-43 37.02635126045037 40.0 36.0 41.0 31.0 41.0 44-45 36.73377003320627 39.5 35.0 41.0 31.0 41.0 46-47 36.34019197710796 39.0 35.0 41.0 30.0 41.0 48-49 35.9716907438846 39.0 35.0 41.0 29.0 41.0 50-51 35.66076513234462 39.0 35.0 40.5 28.0 41.0 52-53 35.39347277832944 38.5 35.0 40.0 27.5 41.0 54-55 35.12591434703226 38.0 34.5 40.0 27.0 41.0 56-57 34.834674610547 38.0 34.0 40.0 26.0 41.0 58-59 34.51652284399458 38.0 34.0 40.0 26.0 41.0 60-61 34.05590377654633 37.0 33.5 40.0 25.0 41.0 62-63 33.7257509849771 36.5 33.0 39.5 24.5 41.0 64-65 33.444019133647245 36.0 33.0 39.0 23.5 41.0 66-67 32.97301429684914 35.5 32.5 39.0 22.5 40.0 68-69 32.55557940143289 35.0 32.0 38.0 22.0 40.0 70-71 32.07328037413114 35.0 31.5 37.5 20.5 39.5 72-73 31.558000149481618 35.0 31.0 37.0 19.5 39.0 74-75 31.115201853572078 35.0 31.0 36.0 18.0 39.0 76-77 29.166924202141857 32.5 28.5 34.5 12.5 36.5 78-79 30.145328379085385 34.0 30.0 35.0 11.5 37.0 80-81 30.17526420876176 34.0 30.0 35.0 10.5 37.0 82-83 29.881887952849226 34.0 30.0 35.0 7.0 36.0 84-85 29.516304387285523 34.0 30.0 35.0 3.5 36.0 86-87 28.96827572952369 34.0 29.0 35.0 2.0 35.5 88-89 28.40133465731339 34.0 29.0 35.0 2.0 35.0 90-91 27.935498681358574 33.0 27.5 35.0 2.0 35.0 92-93 27.609294339985265 33.0 27.0 35.0 2.0 35.0 94-95 27.222830968320572 33.0 26.5 35.0 2.0 35.0 96-97 26.58676169426738 33.0 25.0 34.5 2.0 35.0 98-99 25.70404112879977 32.5 24.0 34.0 2.0 35.0 100-101 21.480126418740724 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1939791852462065 1101 2 0.17196033845670655 1101 3 0.1795649271406461 1101 4 0.09579548852238418 1101 5 0.12887593513936935 1101 6 0.09316298749815388 1101 7 0.20557973023617393 1101 8 0.21478254550400067 1101 9 0.29380164168357936 1101 10-11 0.2609111108861768 1101 12-13 0.16978109415251197 1101 14-15 0.2646169312355653 1101 16-17 0.3404465175797071 1101 18-19 0.34393387471688186 1101 20-21 0.277789840272483 1101 22-23 0.2838929277737705 1101 24-25 0.3958585170036244 1101 26-27 0.36625374131401855 1101 28-29 0.36457625366587365 1101 30-31 0.2639613289185192 1101 32-33 0.35751781830715146 1101 34-35 0.48085940627090906 1101 36-37 0.48734025814572846 1101 38-39 0.49702928529890045 1101 40-41 0.402984892573123 1101 42-43 0.3893349304402065 1101 44-45 0.49946255137570716 1101 46-47 0.5468562253456568 1101 48-49 0.23972715734588235 1101 50-51 0.22252867463675585 1101 52-53 0.4780036477943952 1101 54-55 0.41236872760398313 1101 56-57 0.22028756803202043 1101 58-59 0.3472177139109647 1101 60-61 0.4535635630501744 1101 62-63 0.32433143131597575 1101 64-65 0.2528723521798 1101 66-67 0.24044391768786966 1101 68-69 0.2620270239703615 1101 70-71 0.34540436360794047 1101 72-73 0.3384772899348114 1101 74-75 0.4174570970171061 1101 76-77 0.17453742274664918 1101 78-79 0.30537954812845314 1101 80-81 0.5319131346472759 1101 82-83 0.5039001060354167 1101 84-85 0.33771360124613636 1101 86-87 0.5670309963954203 1101 88-89 0.6200374230266021 1101 90-91 0.4287618969682363 1101 92-93 0.6373321212873684 1101 94-95 0.4442333964690981 1101 96-97 0.4422430729073419 1101 98-99 0.3842735345234978 1101 100-101 1.1291679528316756 1102 1 0.0245503687153672 1102 2 0.07673998140457172 1102 3 0.09969939429010566 1102 4 0.023460157684887406 1102 5 0.13910714034648208 1102 6 0.11122068885531178 1102 7 0.1017889143133317 1102 8 0.16399652275826782 1102 9 0.18134479485286903 1102 10-11 0.21964335510084254 1102 12-13 0.17023771610784877 1102 14-15 0.22765085410331665 1102 16-17 0.27329654235764167 1102 18-19 0.11675914238105634 1102 20-21 0.11621729505795031 1102 22-23 0.15254363384082836 1102 24-25 0.20792391409607092 1102 26-27 0.18719619820249278 1102 28-29 0.15675860677852427 1102 30-31 0.1747460815347779 1102 32-33 0.19597067568202675 1102 34-35 0.19818355580471092 1102 36-37 0.15075875955952966 1102 38-39 0.15472325072639848 1102 40-41 0.1651320152093234 1102 42-43 0.15741970013034035 1102 44-45 0.08940343830087016 1102 46-47 0.1738776051643356 1102 48-49 0.1198604584748324 1102 50-51 0.21703442769860004 1102 52-53 0.3017421593692262 1102 54-55 0.24733736998133793 1102 56-57 0.1348654269685241 1102 58-59 0.10236913000121461 1102 60-61 0.0014261017266576914 1102 62-63 -0.020735885545931865 1102 64-65 0.17382168026048817 1102 66-67 -0.022071230167696854 1102 68-69 0.002360818159417022 1102 70-71 0.14002651050154213 1102 72-73 0.14241188034929309 1102 74-75 0.12424250343771703 1102 76-77 -0.09756251770302171 1102 78-79 -0.03813508751096606 1102 80-81 0.09646288416108462 1102 82-83 0.0715677110709585 1102 84-85 0.25789522976033297 1102 86-87 0.19936505710344576 1102 88-89 0.2612574969023278 1102 90-91 0.2781164213359837 1102 92-93 0.24547687856771105 1102 94-95 0.36886745342919625 1102 96-97 0.34856436152656656 1102 98-99 0.4589598541880129 1102 100-101 0.7367894584139982 1103 1 0.026345090576043617 1103 2 0.16372076212000763 1103 3 0.07431891657804357 1103 4 0.05280322677347726 1103 5 0.053305398243217894 1103 6 0.01300062601201546 1103 7 0.04484537776230724 1103 8 -0.03680965890919197 1103 9 0.05613911719429865 1103 10-11 0.0928227807732327 1103 12-13 0.08530685951698302 1103 14-15 0.1734110287621604 1103 16-17 0.1527425437181691 1103 18-19 0.1561198866663176 1103 20-21 0.1651218564713659 1103 22-23 0.10208294080367608 1103 24-25 0.17898991617091298 1103 26-27 0.115015176114305 1103 28-29 0.09980894454255917 1103 30-31 0.16444278329989004 1103 32-33 0.20422976017660943 1103 34-35 0.2103958874106695 1103 36-37 0.08207913744991657 1103 38-39 0.19939143379425417 1103 40-41 0.2187850206987676 1103 42-43 0.0869128588823429 1103 44-45 0.20026452687037732 1103 46-47 0.27992971958465773 1103 48-49 0.17135628844077644 1103 50-51 0.1391333656086644 1103 52-53 0.20929207643368386 1103 54-55 0.2491008404663333 1103 56-57 0.18529839868572395 1103 58-59 0.2675378417468295 1103 60-61 0.16497140313943248 1103 62-63 0.27461660920674547 1103 64-65 0.3438230382936922 1103 66-67 0.23027314213440775 1103 68-69 0.07006219953251502 1103 70-71 0.05931365520143572 1103 72-73 0.13580621166121887 1103 74-75 0.056538111134731395 1103 76-77 -0.03162678714345191 1103 78-79 0.20751235378005717 1103 80-81 0.23091948814780494 1103 82-83 0.11584011259693838 1103 84-85 0.3086589884639963 1103 86-87 0.1824472650411728 1103 88-89 0.22797945393199726 1103 90-91 0.3921988934049345 1103 92-93 0.3720809774192375 1103 94-95 0.29935795674869325 1103 96-97 0.2886969005835205 1103 98-99 0.21990136573295516 1103 100-101 0.20875157551715873 1104 1 -0.04884541612053539 1104 2 -0.038518021452176754 1104 3 -0.03911388834909957 1104 4 -0.03313389644344511 1104 5 -0.015087618391383728 1104 6 -0.028021522752418093 1104 7 -0.04453416498022733 1104 8 0.003848604729512317 1104 9 0.06510004715055118 1104 10-11 0.018997671250637893 1104 12-13 0.07456469324134929 1104 14-15 0.049127181805047826 1104 16-17 -0.007617263741479974 1104 18-19 0.022977154899123775 1104 20-21 -0.011048428520233244 1104 22-23 -0.015651491360621606 1104 24-25 -0.07726221192576332 1104 26-27 -0.026456964836327757 1104 28-29 -0.043396411482191866 1104 30-31 -0.03171065285824426 1104 32-33 0.0087706656875568 1104 34-35 0.029606558890499457 1104 36-37 -0.06915176918912636 1104 38-39 0.02800878415995811 1104 40-41 0.12530835498770188 1104 42-43 0.02851488488212084 1104 44-45 0.25003202745770636 1104 46-47 0.27373549641627193 1104 48-49 0.10236952636637398 1104 50-51 -0.009817566821105572 1104 52-53 -0.07484373261562638 1104 54-55 -0.0012986983641241068 1104 56-57 0.04693904866983445 1104 58-59 0.11828832894513397 1104 60-61 0.2114223329818401 1104 62-63 0.15918336753465212 1104 64-65 0.09114114489050706 1104 66-67 0.11265348488605298 1104 68-69 0.22512497385645958 1104 70-71 0.1939237257947326 1104 72-73 0.13301031954756937 1104 74-75 0.0851108555592468 1104 76-77 0.02601806055985989 1104 78-79 0.009935356784488647 1104 80-81 0.1398857997953371 1104 82-83 0.10686138166184023 1104 84-85 0.07689355568804856 1104 86-87 -0.23547469836539747 1104 88-89 -0.044882850321918966 1104 90-91 0.19049310425753063 1104 92-93 0.22343690488645862 1104 94-95 0.20118100466955013 1104 96-97 0.2978239385109873 1104 98-99 0.3362097532023114 1104 100-101 0.7754094106904432 1105 1 0.022159941681209716 1105 2 0.014870932998888975 1105 3 0.034113671345515684 1105 4 -0.036644979500046304 1105 5 0.030466402297804507 1105 6 0.0818497009426622 1105 7 0.007921672035259064 1105 8 0.010059629542503501 1105 9 -0.048484536692285474 1105 10-11 -0.004689890944483466 1105 12-13 0.03467188993499093 1105 14-15 0.14649757962004628 1105 16-17 0.06971347150017948 1105 18-19 0.08070205602221847 1105 20-21 0.036217425582073304 1105 22-23 0.032477343899422806 1105 24-25 0.11487693438360225 1105 26-27 0.07874074323209612 1105 28-29 -0.027408796125797608 1105 30-31 -0.0355965075907676 1105 32-33 -0.15578001997079127 1105 34-35 0.0563205522976844 1105 36-37 0.08052280864638561 1105 38-39 0.068898844019742 1105 40-41 0.14792760953054795 1105 42-43 0.06150322904591832 1105 44-45 0.05690690738745019 1105 46-47 0.018651480270698073 1105 48-49 -0.1807902450910035 1105 50-51 -0.018244898013755062 1105 52-53 0.07565560718853703 1105 54-55 0.06845823369211956 1105 56-57 0.03446922505749228 1105 58-59 -0.16916398600944405 1105 60-61 -0.1669036258776444 1105 62-63 -0.13048240768976171 1105 64-65 -0.14632494320268563 1105 66-67 0.0012091756318213243 1105 68-69 0.012832422227525342 1105 70-71 -0.014073537873969144 1105 72-73 -0.05276441695856704 1105 74-75 -0.1523483205529459 1105 76-77 -0.3559773470386425 1105 78-79 -0.09099572771549802 1105 80-81 -0.10726600662039942 1105 82-83 -0.055040998804091856 1105 84-85 -0.26459662675775775 1105 86-87 -0.16058795828942962 1105 88-89 0.046089695899411254 1105 90-91 0.06442619424847251 1105 92-93 0.07600404512546888 1105 94-95 0.26506793685793895 1105 96-97 0.2840595415152585 1105 98-99 0.5031918298256848 1105 100-101 1.0839146033161704 1106 1 -0.03155169412060843 1106 2 -0.1062826832172945 1106 3 -0.11075437839772917 1106 4 -0.02779697803710235 1106 5 0.0023846258829394174 1106 6 -0.05240946063065621 1106 7 -0.008743939623279573 1106 8 -0.08696477865655083 1106 9 -0.126329287948721 1106 10-11 -0.043901710005116 1106 12-13 0.014419641400074568 1106 14-15 0.04448171307934956 1106 16-17 -0.024311489992030033 1106 18-19 -0.07030242893453931 1106 20-21 -0.09359214154418538 1106 22-23 0.02140923413794127 1106 24-25 0.046276398752070236 1106 26-27 0.0033398987566997107 1106 28-29 0.053389489446921345 1106 30-31 0.05211891920690448 1106 32-33 0.03367482216483353 1106 34-35 0.010790211436287223 1106 36-37 -0.2710292450636871 1106 38-39 0.013193828980014644 1106 40-41 0.10166931306388705 1106 42-43 0.04168773433698192 1106 44-45 -0.0019678209479607744 1106 46-47 0.05400988216002389 1106 48-49 0.07290716763833416 1106 50-51 0.00104416717871203 1106 52-53 0.07537368557329671 1106 54-55 0.1815322460286879 1106 56-57 0.15457730868603647 1106 58-59 0.03910253014273479 1106 60-61 0.19662911962079477 1106 62-63 0.17622965167828397 1106 64-65 0.03569043100087299 1106 66-67 0.10228403353785609 1106 68-69 0.09504676314832494 1106 70-71 0.00769108434325716 1106 72-73 0.1391533807158858 1106 74-75 0.1432992434332938 1106 76-77 -0.18475919949224107 1106 78-79 -0.019752299048501953 1106 80-81 0.06187924533507427 1106 82-83 0.07269870099530351 1106 84-85 -0.03042555836275085 1106 86-87 0.033776286000438915 1106 88-89 0.22808299308113789 1106 90-91 0.3995521622184235 1106 92-93 0.4109908106346758 1106 94-95 0.4051203629468034 1106 96-97 0.3438406976634951 1106 98-99 0.3956060088222735 1106 100-101 0.7537707158378701 1107 1 -0.054840679025382144 1107 2 -0.07161167563634763 1107 3 -0.10811074342236537 1107 4 -0.015020418974209804 1107 5 -0.14943274060212985 1107 6 0.00513164069706562 1107 7 -0.12276717331152298 1107 8 0.013782711026458117 1107 9 -0.03939763159399945 1107 10-11 -0.059598008613164666 1107 12-13 0.021276806332600984 1107 14-15 0.009313414277400511 1107 16-17 -0.07495603698359332 1107 18-19 -0.039665805273024546 1107 20-21 -0.06332327856476638 1107 22-23 -0.02512252887117228 1107 24-25 -0.07068210299621569 1107 26-27 -0.12975992611195153 1107 28-29 -0.002175703046063404 1107 30-31 -0.14500762379722687 1107 32-33 -0.09049118296184844 1107 34-35 0.04199305777519413 1107 36-37 0.007948099969311784 1107 38-39 0.013154818320636252 1107 40-41 -0.01613984830541426 1107 42-43 -0.021479991803644793 1107 44-45 -0.09760908043929106 1107 46-47 -0.27552792863752273 1107 48-49 -0.2358106921232661 1107 50-51 -0.28667762546334785 1107 52-53 -0.20710287773153624 1107 54-55 -0.27161746703465894 1107 56-57 -0.11512231681164309 1107 58-59 -0.13994503407715086 1107 60-61 -0.2374966477150906 1107 62-63 -0.20972363248899484 1107 64-65 -0.2709996319615726 1107 66-67 -0.15576049466798736 1107 68-69 -0.17537967259544018 1107 70-71 -0.14091490951199503 1107 72-73 -0.1119082394611901 1107 74-75 -0.22006989890789086 1107 76-77 -0.5489551405656918 1107 78-79 -0.20312436529541955 1107 80-81 -0.019339811258408446 1107 82-83 -0.240300105202774 1107 84-85 -0.26944879205099426 1107 86-87 -0.2952706722286145 1107 88-89 -0.08851880731760531 1107 90-91 0.03612961693576011 1107 92-93 0.048471191681706216 1107 94-95 0.24668519790520094 1107 96-97 0.24052380688122454 1107 98-99 0.1735160525142092 1107 100-101 0.8247515574379278 1108 1 -0.02588927035908739 1108 2 -0.061940333514890256 1108 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#Quality Count 2 2.0 3 9.0 4 28.0 5 91.0 6 244.0 7 508.0 8 953.0 9 1743.0 10 2783.0 11 4236.0 12 6072.0 13 8650.0 14 10284.0 15 13550.0 16 17591.0 17 24726.0 18 23880.0 19 15813.0 20 14678.0 21 15590.0 22 15828.0 23 17230.0 24 20137.0 25 23317.0 26 28166.0 27 34064.0 28 41050.0 29 50026.0 30 60556.0 31 73267.0 32 90975.0 33 115293.0 34 159638.0 35 232696.0 36 351696.0 37 509625.0 38 335845.0 39 20585.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34122740703928 18.973563164667528 14.71634634556944 21.968863082723757 2 16.157767171700993 21.787885582497836 37.69939246399095 24.35495478181022 3 20.450964690306115 24.66378380686975 30.84373832174851 24.041513181075626 4 13.228653490929668 17.0016549750686 36.552783027429875 33.216908506571855 5 13.559199697276943 37.8013029733315 33.67008141234314 14.969415917048416 6 31.090468411330708 35.895277448562304 16.7320755522812 16.28217858782579 7 27.499663666357026 34.09359684807329 20.446394823665077 17.96034466190461 8 26.131223507052333 33.02937313815305 19.667552879122756 21.171850475671867 9 26.033291692025156 17.358830626648302 21.119062109612734 35.48881557171381 10-11 25.353855024183993 25.634175769029543 26.61377152802247 22.398197678764 12-13 27.542863854276778 23.60069188635126 27.057112655754505 21.799331603617457 14-15 23.58666017765901 24.914116389136307 27.259726090701037 24.23949734250365 16-17 21.774269841320688 28.269113110663362 27.645628657733816 22.310988390282134 18-19 21.42631090041321 27.287762794025006 29.74297703321695 21.54294927234483 20-21 22.75035501884536 25.643721238134898 29.209946934025222 22.395976808994522 22-23 22.182068612063166 25.561741247317336 29.436262105341832 22.819928035277663 24-25 22.912884247840523 25.76356278761865 29.481490972377934 21.842061992162893 26-27 22.818988436529036 25.239437522021845 30.451754807435645 21.489819234013474 28-29 21.522000491153893 26.492819543653972 30.270134640229774 21.71504532496236 30-31 21.9017265127006 26.046467429022925 29.82886490064811 22.222941157628366 32-33 21.932028572343764 26.3003726363219 29.739667083079745 22.027931708254588 34-35 22.916836171946155 25.56750367572866 29.32085774476295 22.194802407562232 36-37 22.992139235142968 25.325552867530483 29.924232716909955 21.758075180416597 38-39 22.653173720577442 25.645368283489407 29.900139868682764 21.801318127250386 40-41 22.250816561653476 26.055593460673173 29.9187122083308 21.774877769342556 42-43 21.943660492044973 26.596728811503485 28.77577718060726 22.683833515844285 44-45 22.234674331547378 26.26053049117734 28.573469461842514 22.931325715432763 46-47 22.852879267094483 26.71132758938431 27.94030376004652 22.49548938347469 48-49 23.06266971553136 26.32231835579978 28.09882729183768 22.516184636831184 50-51 22.22900824651434 26.862598169866192 28.526593017423025 22.381800566196443 52-53 22.57454328026736 27.36436144655498 27.738898320467236 22.32219695271042 54-55 21.74684220079652 26.434628484790245 28.22983866662396 23.588690647789274 56-57 22.647789273626103 27.041139477027876 27.38509668257578 22.925974566770236 58-59 23.305364526966137 26.46460392804702 27.430578393093413 22.799453151893427 60-61 22.48432484561887 26.444237964656935 28.5632587322478 22.5081784574764 62-63 22.53672443063519 26.401806592139405 28.341843108363495 22.71962586886191 64-65 22.102964057266128 27.07191664541887 28.287119904639024 22.53799939267598 66-67 22.100426178193608 26.590021361228995 27.87039245679332 23.439160003784075 68-69 21.454000478775733 26.97057750134162 28.306921299049687 23.268500720832957 70-71 22.32988457883554 26.917368696413508 27.44119499877212 23.311551725978838 72-73 22.078627331646327 26.253157799203475 27.9801616536938 23.6880532154564 74-75 21.543141462997962 27.59328186894733 27.38475501030355 23.478821657751155 76-77 22.075747876542483 26.473057116014783 27.564580348006285 23.886614659436457 78-79 21.839966550255976 26.45466026429227 28.010274094001808 23.695099091449944 80-81 21.897946763189083 27.217207469810052 27.56058810339857 23.324257663602292 82-83 21.603873709386377 26.771218381968247 27.895106612426197 23.729801296219183 84-85 21.552387968865112 26.54883244178225 27.426033291692026 24.472746297660613 86-87 21.65014396945076 26.600387682442573 27.64095832499286 24.108510023113812 88-89 21.69079567271549 26.37827254805693 27.32953386479563 24.601397914431953 90-91 21.498752914152497 26.033131342567195 27.814342772901814 24.653772970378498 92-93 21.44354399564368 26.54817045175481 27.694822597349905 24.313462955251612 94-95 21.28221061960131 26.278078520559063 27.60464247199889 24.83506838784074 96-97 20.4984603294596 26.11708329596269 27.89801871521861 25.486437659359108 98-99 21.06253670307612 26.25945738172267 27.096832057400945 25.58117385780027 100-101 21.071854061179554 25.444500654410714 27.942387086124466 25.54125819828527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5905.0 1 3854.0 2 1980.5 3 2104.5 4 1761.0 5 1737.0 6 2894.5 7 5464.5 8 7852.5 9 8416.5 10 6794.5 11 4941.5 12 4624.5 13 4682.5 14 4774.0 15 4992.0 16 5071.5 17 5184.5 18 5465.5 19 5814.5 20 6353.5 21 7113.5 22 8034.0 23 9166.5 24 10679.5 25 12413.0 26 14514.5 27 16402.5 28 17686.5 29 19077.0 30 21175.0 31 23335.0 32 25741.5 33 28810.5 34 31875.5 35 34328.0 36 38393.5 37 43437.5 38 46059.5 39 46875.5 40 49016.0 41 54114.0 42 58554.0 43 62339.0 44 65008.0 45 67916.0 46 73786.5 47 93103.5 48 145812.0 49 149514.0 50 104074.0 51 85752.5 52 78309.5 53 77748.5 54 78793.0 55 75529.5 56 68444.5 57 61746.0 58 56977.5 59 51987.0 60 45858.5 61 38991.5 62 33662.5 63 29162.0 64 23854.0 65 18440.5 66 12676.0 67 9525.0 68 6463.0 69 3258.5 70 2334.5 71 1749.0 72 1285.5 73 967.5 74 701.5 75 526.0 76 326.0 77 154.5 78 72.5 79 28.0 80 12.5 81 5.0 82 3.0 83 1.0 84 2.5 85 2.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3988169597574127 2 0.0 3 0.0 4 0.0 5 4.697993743126515E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012599165038384744 16-17 0.002882859796918543 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022422242864922003 36-37 0.007559499023030847 38-39 0.01283406472554107 40-41 0.0022422242864922003 42-43 5.552174423694971E-4 44-45 4.2709034028422867E-4 46-47 1.9219065312790285E-4 48-49 2.9896323819896003E-4 50-51 4.911538913268629E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.9896323819896003E-4 60-61 0.002583896558719583 62-63 0.0 64-65 2.5625420417053717E-4 66-67 0.0014307526399521659 68-69 0.0015588797420374345 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12402703481854 78-79 8.541806805684572E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.146449277683462 88-89 0.4403087863160255 90-91 0.3410316367169566 92-93 0.0 94-95 0.0 96-97 6.406355104263429E-4 98-99 0.0 100-101 2.3489968715632574E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2341425.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.715282698691745 #Duplication Level Percentage of deduplicated Percentage of total 1 63.43324054194094 21.386696373656534 2 15.547243766662493 10.48359437556998 3 7.156763955219529 7.238769598741011 4 3.9196676214191624 5.286108077642228 5 2.382661018584855 4.016604490837061 6 1.5810434002848377 3.19831951197025 7 1.1562788857693402 2.7288988658569124 8 0.8105890132635869 2.186339018770834 9 0.6042819058684147 1.8336181757452052 >10 3.0813025090285113 18.307203794503433 >50 0.19435610849702362 4.530855122927959 >100 0.11785804908826093 7.371106874512906 >500 0.006595583339746626 1.4993856829821783 >1k 0.006722421480895598 5.228050233509304 >5k 8.878669880428148E-4 2.2277821332199124 >10k+ 5.073525645958942E-4 2.476667669554299 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18100 0.7730335159144538 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15401 0.6577618330717405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13773 0.5882315256734681 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10641 0.45446683109644764 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9596 0.4098358905367458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9309 0.3975783977705884 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9152 0.39087307942812605 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7203 0.30763317210672986 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6110 0.2609521979136637 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5667 0.24203209583907237 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5058 0.21602229411576282 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4757 0.20316687487320753 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4716 0.20141580447804222 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4693 0.2004334966953885 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4580 0.1956073758501767 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4342 0.18544262575141207 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4329 0.18488740830904257 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4229 0.1806165049062003 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4224 0.1804029597360582 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3906 0.1668214869150197 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3677 0.15704111812251087 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3560 0.15204416114118538 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3327 0.14209295621256285 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3121 0.13329489520270774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2987 0.1275718846428991 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2809 0.11996967658583983 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2786 0.1189873688031861 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2727 0.11646753579550914 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2502 0.106858003139114 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2466 0.10532047791409077 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2443 0.10433817013143704 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2439 0.10416733399532337 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2383 0.10177562808973167 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.270903402842286E-5 0.0 8 0.0 0.0 0.0 4.270903402842286E-5 0.0 9 0.0 0.0 0.0 4.270903402842286E-5 0.0 10-11 0.0 0.0 0.0 4.270903402842286E-5 0.0 12-13 0.0 0.0 0.0 4.270903402842286E-5 0.0 14-15 0.0 0.0 0.0 8.541806805684572E-5 0.0 16-17 0.0 0.0 0.0 1.9219065312790288E-4 0.0 18-19 0.0 0.0 0.0 2.9896323819896003E-4 0.0 20-21 0.0 0.0 0.0 5.765719593837087E-4 0.0 22-23 0.0 0.0 0.0 0.0011531439187674173 0.0 24-25 0.0 0.0 0.0 0.0020073245993358748 0.0 26-27 0.0 0.0 0.0 0.003950585647629115 0.0 28-29 0.0 0.0 0.0 0.011659566289759442 0.0 30-31 0.0 0.0 0.0 0.030835922568521308 0.0 32-33 0.0 0.0 0.0 0.0629531161578953 0.0 34-35 0.0 0.0 0.0 0.1173003619590634 0.0 36-37 2.135451701421143E-5 0.0 0.0 0.1997074431169053 0.0 38-39 4.270903402842286E-5 0.0 0.0 0.3054550113712803 0.0 40-41 4.270903402842286E-5 0.0 0.0 0.42029960387370935 0.0 42-43 4.270903402842286E-5 0.0 0.0 0.5377708019688865 0.0 44-45 4.270903402842286E-5 0.0 0.0 0.6807606478960462 0.0 46-47 4.270903402842286E-5 0.0 0.0 0.857383858120589 0.0 48-49 4.270903402842286E-5 0.0 0.0 1.0376373362375477 0.0 50-51 4.270903402842286E-5 0.0 0.0 1.2284399457595268 0.0 52-53 4.270903402842286E-5 0.0 0.0 1.4089496780806559 0.0 54-55 4.270903402842286E-5 0.0 0.0 1.6112196632392668 0.0 56-57 4.270903402842286E-5 0.0 0.0 1.8617935658840237 0.0 58-59 4.270903402842286E-5 0.0 0.0 2.132227169351997 0.0 60-61 4.270903402842286E-5 0.0 0.0 2.4541465133412346 0.0 62-63 4.270903402842286E-5 0.0 0.0 2.76596517078275 0.0 64-65 4.270903402842286E-5 0.0 0.0 3.073961369678721 0.0 66-67 4.270903402842286E-5 0.0 0.0 3.4321406835580897 0.0 68-69 4.270903402842286E-5 0.0 0.0 3.8422755373330344 0.0 70-71 4.270903402842286E-5 0.0 0.0 4.296422050674269 0.0 72-73 4.270903402842286E-5 0.0 0.0 4.752810788301995 0.0 74-75 4.270903402842286E-5 0.0 0.0 5.241316719519096 0.0 76-77 4.270903402842286E-5 0.0 0.0 5.742272334155482 0.0 78-79 4.270903402842286E-5 0.0 0.0 6.327813190685159 0.0 80-81 1.2812710208526858E-4 0.0 0.0 6.963024653789892 0.0 82-83 1.2812710208526858E-4 0.0 0.0 7.5876229219385625 0.0 84-85 1.2812710208526858E-4 0.0 0.0 8.265479355520675 0.0 86-87 1.2812710208526858E-4 0.0 0.0 8.987646411907278 0.0 88-89 1.2812710208526858E-4 0.0 0.0 9.726726245769136 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGT 85 6.445779E-4 27.91398 3 TTTGCGT 725 0.0 25.526852 3 GTAAGCG 240 0.0 23.72739 94-95 GAGTACT 11520 0.0 22.862816 12-13 GTACATG 10555 0.0 22.61164 1 TACATGG 10835 0.0 21.591793 2 CATGGGG 4095 0.0 21.554092 4 CGTATAG 155 3.8222322E-5 21.51389 1 GTGTACG 200 5.242746E-7 21.437056 1 CTTTGCG 895 0.0 21.208387 2 ACATGGG 10835 0.0 20.847248 3 TTGCGTT 935 0.0 20.808603 4 GTACTTT 12860 0.0 20.462082 14-15 TGTACGC 120 0.0047918633 19.772404 2 AGTACTT 12135 0.0 19.768358 12-13 GAGTGCA 1830 0.0 19.44868 9 AGAGTGC 1970 0.0 18.066154 8 ACTTTTT 14525 0.0 18.034258 16-17 CGGTTGC 185 1.5335798E-4 17.955479 6 TACTTTT 13575 0.0 17.531551 14-15 >>END_MODULE