##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939023_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1705297 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11464513219691 33.0 31.0 34.0 30.0 34.0 2 32.3369588992416 34.0 31.0 34.0 30.0 34.0 3 32.4092067246937 34.0 31.0 34.0 30.0 34.0 4 35.971234922714345 37.0 35.0 37.0 35.0 37.0 5 35.91330659703266 37.0 35.0 37.0 35.0 37.0 6 35.98328795511867 37.0 35.0 37.0 35.0 37.0 7 35.92175146030281 37.0 35.0 37.0 35.0 37.0 8 35.91035755062021 37.0 35.0 37.0 35.0 37.0 9 37.5389342736192 39.0 37.0 39.0 35.0 39.0 10-11 37.453229847938516 39.0 37.0 39.0 34.0 39.0 12-13 37.37400142028045 39.0 37.0 39.0 34.0 39.0 14-15 38.56340068621478 40.0 38.0 41.0 34.0 41.0 16-17 38.70307899445082 40.0 38.0 41.0 34.0 41.0 18-19 38.79250564564413 40.0 38.0 41.0 34.0 41.0 20-21 38.77559803365631 40.0 38.0 41.0 34.0 41.0 22-23 38.69176718190438 40.0 38.0 41.0 34.0 41.0 24-25 38.576678725172215 40.0 38.0 41.0 34.0 41.0 26-27 38.37724425715872 40.0 38.0 41.0 34.0 41.0 28-29 38.22612805863143 40.0 38.0 41.0 34.0 41.0 30-31 38.00179792728187 40.0 37.0 41.0 33.0 41.0 32-33 37.78915285724422 40.0 37.0 41.0 33.0 41.0 34-35 37.59970609225255 40.0 36.0 41.0 33.0 41.0 36-37 37.41002095236196 40.0 36.0 41.0 32.5 41.0 38-39 37.22971804911403 40.0 36.0 41.0 32.0 41.0 40-41 37.089258352064185 40.0 36.0 41.0 31.5 41.0 42-43 36.82282880929246 40.0 35.0 41.0 31.0 41.0 44-45 36.471222021735805 39.0 35.0 41.0 30.0 41.0 46-47 36.01382222568854 39.0 35.0 41.0 29.5 41.0 48-49 35.53777670399936 39.0 35.0 41.0 27.0 41.0 50-51 35.219026656353705 39.0 35.0 40.0 26.5 41.0 52-53 35.00407846844274 38.5 35.0 40.0 26.0 41.0 54-55 34.72616617515893 38.0 34.0 40.0 25.0 41.0 56-57 34.56605007808024 38.0 34.0 40.0 24.5 41.0 58-59 34.293393467530876 38.0 34.0 40.0 24.0 41.0 60-61 33.854895657471985 37.5 33.5 40.0 22.5 41.0 62-63 33.49254821887331 37.0 33.0 40.0 22.0 41.0 64-65 33.10255339685697 36.0 33.0 39.0 21.5 40.5 66-67 32.597173102398 36.0 32.0 39.0 19.5 40.0 68-69 32.07279230538727 35.0 31.5 38.5 17.5 40.0 70-71 31.492216018675926 35.0 31.0 37.5 13.0 39.5 72-73 30.96960294892913 35.0 30.5 37.0 8.0 39.0 74-75 30.474844264664746 34.0 30.0 36.5 7.0 39.0 76-77 28.61396460557897 32.5 27.5 34.5 2.0 36.5 78-79 29.569624821951834 34.0 29.0 35.0 2.0 37.0 80-81 29.515209667289625 34.0 29.0 35.0 2.0 37.0 82-83 29.09435423858718 34.0 29.0 35.0 2.0 36.5 84-85 28.635650564095286 34.0 29.0 35.0 2.0 36.0 86-87 28.01774676200099 33.5 27.5 35.0 2.0 35.5 88-89 27.320975466443677 33.0 26.0 35.0 2.0 35.0 90-91 26.72847662313368 32.5 25.0 34.5 2.0 35.0 92-93 26.344017493726902 32.0 25.0 34.0 2.0 35.0 94-95 25.793789293008786 32.0 23.5 34.0 2.0 35.0 96-97 24.928220128223998 32.0 19.0 34.0 2.0 35.0 98-99 23.81089745657208 31.0 2.0 34.0 2.0 35.0 100-101 18.46349198995835 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29005083738463355 1101 2 0.28344537319305374 1101 3 0.2537317657965872 1101 4 0.20133329627314112 1101 5 0.1633603727071673 1101 6 0.14555672464167202 1101 7 0.191934308265445 1101 8 0.167358491731477 1101 9 0.253198920809929 1101 10-11 0.31432205354281706 1101 12-13 0.3489322108701529 1101 14-15 0.41074167259240824 1101 16-17 0.39547186950480295 1101 18-19 0.46155985855775583 1101 20-21 0.4170468677476933 1101 22-23 0.408656982455156 1101 24-25 0.3891519922678981 1101 26-27 0.2376390729907527 1101 28-29 0.40598400560391923 1101 30-31 0.3962324473222907 1101 32-33 0.5441421751244704 1101 34-35 0.5179544594603058 1101 36-37 0.606848689312919 1101 38-39 0.5749530672339844 1101 40-41 0.7546766634386586 1101 42-43 0.7304884628472976 1101 44-45 0.7777337525174062 1101 46-47 0.6835224782053118 1101 48-49 0.6203397965722601 1101 50-51 0.5093923090415089 1101 52-53 0.4942616855908142 1101 54-55 0.4680721551236715 1101 56-57 0.3388889999326423 1101 58-59 0.38938940544723977 1101 60-61 0.43761527463590255 1101 62-63 0.4672770207836834 1101 64-65 0.4400321003460661 1101 66-67 0.13985751288756632 1101 68-69 0.2354895084341493 1101 70-71 0.5273033087353056 1101 72-73 0.4713830707480007 1101 74-75 0.5010853392372461 1101 76-77 0.21758478566693995 1101 78-79 0.6770652043840002 1101 80-81 0.6464320850894403 1101 82-83 0.6133145284102213 1101 84-85 0.5501648426631043 1101 86-87 0.5971917502663118 1101 88-89 0.7220176356563677 1101 90-91 0.6237109662352829 1101 92-93 0.7544469722608049 1101 94-95 0.7210758346397803 1101 96-97 0.7454134946578357 1101 98-99 1.0836565245826826 1101 100-101 1.3648017536084787 1102 1 0.033397756588655625 1102 2 0.06094843667369787 1102 3 0.04231663752998571 1102 4 9.017450863737508E-4 1102 5 -0.01082181115495473 1102 6 -0.010407738982820547 1102 7 0.005578118702587176 1102 8 0.05972709505069673 1102 9 -0.0011632092414828321 1102 10-11 0.07249438764868188 1102 12-13 0.13571395785329088 1102 14-15 0.15492189183106575 1102 16-17 0.142554903149815 1102 18-19 0.06079748475983848 1102 20-21 0.12335314255523144 1102 22-23 0.11990561720834592 1102 24-25 0.04449543508737008 1102 26-27 0.08136992623671091 1102 28-29 0.02624476774463602 1102 30-31 -0.15207148325657727 1102 32-33 -0.004012743759894022 1102 34-35 0.07842823340207872 1102 36-37 0.10472756195203914 1102 38-39 0.20667441037046075 1102 40-41 0.08288948876698754 1102 42-43 0.1503326582174509 1102 44-45 0.1825718875236646 1102 46-47 0.3469325317496299 1102 48-49 0.4445201490057258 1102 50-51 0.47328236338498897 1102 52-53 0.30347593610362367 1102 54-55 0.3375695445053779 1102 56-57 0.41695364603403107 1102 58-59 0.30409810839617535 1102 60-61 0.35229567368755 1102 62-63 0.48639047612346786 1102 64-65 0.3505168628597204 1102 66-67 0.33766512630294443 1102 68-69 0.27584337374756274 1102 70-71 0.3950758253180595 1102 72-73 0.3818819852102955 1102 74-75 0.48632901914347926 1102 76-77 0.1110256072125324 1102 78-79 0.40594550220760084 1102 80-81 0.3812170753662194 1102 82-83 0.34174945608543794 1102 84-85 0.5935056016071378 1102 86-87 0.579318671601456 1102 88-89 0.4855835271292328 1102 90-91 0.5337886521086403 1102 92-93 0.8176104489117542 1102 94-95 0.806013332974846 1102 96-97 0.7451679167255527 1102 98-99 0.7855224173874937 1102 100-101 1.1863065552814085 1103 1 0.08474195453072753 1103 2 0.09692093150243863 1103 3 0.11166773489512138 1103 4 0.11936662345430449 1103 5 0.1268527396225565 1103 6 0.12656629565967847 1103 7 0.12507890182676107 1103 8 0.09372691000869793 1103 9 0.16001332743317676 1103 10-11 0.12733207938192237 1103 12-13 0.14860328952125457 1103 14-15 0.2454776456459058 1103 16-17 0.1634043450499263 1103 18-19 0.28933509124159684 1103 20-21 0.2974264456209781 1103 22-23 0.16214888795783367 1103 24-25 0.22072811218247068 1103 26-27 0.13707252409418658 1103 28-29 0.20298465817332811 1103 30-31 0.17936764783907222 1103 32-33 0.25714181917751233 1103 34-35 0.24631749898360766 1103 36-37 0.21896538717987113 1103 38-39 0.27398508927633003 1103 40-41 0.3350984276172113 1103 42-43 0.18013268068683175 1103 44-45 0.3007079859131423 1103 46-47 0.4316834189599277 1103 48-49 0.3991162948452498 1103 50-51 0.5344527870650779 1103 52-53 0.4974643841960358 1103 54-55 0.4684264541718406 1103 56-57 0.49200139347814087 1103 58-59 0.4652159933661366 1103 60-61 0.29805275980477575 1103 62-63 0.4440910319103182 1103 64-65 0.4321732530868516 1103 66-67 0.2622241646132508 1103 68-69 0.16521882396135368 1103 70-71 0.2952473195982819 1103 72-73 0.349032074096538 1103 74-75 0.23735666309623937 1103 76-77 0.1855264894663442 1103 78-79 0.4181648827134232 1103 80-81 0.2887968582062186 1103 82-83 0.4769003314001736 1103 84-85 0.8402706709648449 1103 86-87 0.998088538833823 1103 88-89 0.7210585563724798 1103 90-91 0.5687871982072288 1103 92-93 0.7730971093663008 1103 94-95 1.0606220352356601 1103 96-97 0.8778562465232618 1103 98-99 1.1389856766379438 1103 100-101 1.3027376238328578 1104 1 0.01893081862864676 1104 2 0.07121906076265816 1104 3 0.06412171766925923 1104 4 0.02592414882376204 1104 5 0.04786047365082169 1104 6 0.07392417259056572 1104 7 0.056725973575339594 1104 8 0.0176452042895221 1104 9 0.06863320648700011 1104 10-11 0.06484732690476136 1104 12-13 0.06543845309587226 1104 14-15 0.10914338440151283 1104 16-17 0.16505168337771892 1104 18-19 0.03662062826944634 1104 20-21 0.15129741805345986 1104 22-23 0.043859211681926524 1104 24-25 0.0661701328205524 1104 26-27 0.014888569900570303 1104 28-29 0.07645566485719968 1104 30-31 0.0946703848804944 1104 32-33 0.19999470946159192 1104 34-35 0.3636563760873557 1104 36-37 0.32372939995628514 1104 38-39 0.3248671837164565 1104 40-41 0.4308015747059315 1104 42-43 0.3134033005885115 1104 44-45 0.4067810641597873 1104 46-47 0.4785759132203893 1104 48-49 0.3572686101260629 1104 50-51 0.3517555548034963 1104 52-53 0.3554313865370844 1104 54-55 0.6660442458296885 1104 56-57 0.4227225063897819 1104 58-59 0.33094953856262777 1104 60-61 0.4830696350764043 1104 62-63 0.35313986114291396 1104 64-65 0.3785439791355216 1104 66-67 0.35631491140662064 1104 68-69 0.3458020023286892 1104 70-71 0.2541404019486393 1104 72-73 0.22524884661399724 1104 74-75 0.22425752632818075 1104 76-77 0.30350737816063855 1104 78-79 0.367762024984323 1104 80-81 0.3970066091889244 1104 82-83 0.5143512767869822 1104 84-85 0.7141199373873341 1104 86-87 0.7605198139064058 1104 88-89 0.7243075914365313 1104 90-91 0.6900710436688406 1104 92-93 0.7471682423925543 1104 94-95 1.0828919006373958 1104 96-97 0.9259701582419204 1104 98-99 1.1945787507485797 1104 100-101 0.9958231650613527 1105 1 0.035870028502792195 1105 2 0.023144421567359075 1105 3 -0.005228979643540299 1105 4 0.04665129785918509 1105 5 0.01155783503864427 1105 6 -0.058349698831762 1105 7 0.07285659216710627 1105 8 0.1396320841184746 1105 9 0.16072786609144885 1105 10-11 0.13309029303528064 1105 12-13 0.09593458993439441 1105 14-15 0.13299682723157957 1105 16-17 0.032491695040093305 1105 18-19 0.08836774912079903 1105 20-21 0.14182620953992142 1105 22-23 0.05212764066292408 1105 24-25 0.02147039036464804 1105 26-27 -0.08258574857943302 1105 28-29 0.11360751868877372 1105 30-31 0.09808435057367149 1105 32-33 0.09024962952573645 1105 34-35 0.14152702091946878 1105 36-37 0.20503267028040284 1105 38-39 0.19487019649647408 1105 40-41 0.2720824921460405 1105 42-43 0.26670793152295147 1105 44-45 -0.04485526413603935 1105 46-47 0.1087548334789048 1105 48-49 0.1700818663581387 1105 50-51 0.15117204734443845 1105 52-53 0.09088519867567157 1105 54-55 0.1305824644020177 1105 56-57 0.1766401894647629 1105 58-59 0.0920444411332042 1105 60-61 -0.039622235276866036 1105 62-63 -0.029399426480388513 1105 64-65 -0.013417146283593695 1105 66-67 0.08477265959652414 1105 68-69 0.1045436321455 1105 70-71 0.32472400659415257 1105 72-73 0.12453394307155108 1105 74-75 0.09070429244898648 1105 76-77 -0.11485930632354524 1105 78-79 0.03623610049819703 1105 80-81 0.01610238643757711 1105 82-83 0.02938213348556218 1105 84-85 0.2009834390152072 1105 86-87 0.2878424243345137 1105 88-89 0.34624840488713815 1105 90-91 0.054261917860976894 1105 92-93 0.356489200650973 1105 94-95 0.3778024405081375 1105 96-97 0.5636060006054961 1105 98-99 0.8727098552726069 1105 100-101 0.9618281215704165 1106 1 0.06719550782827355 1106 2 0.05897902540196753 1106 3 0.07529030887575061 1106 4 0.06519045239834043 1106 5 0.07907273455354868 1106 6 -0.0034030598851231275 1106 7 0.15066855797907408 1106 8 0.07551381200020302 1106 9 0.061163070526653485 1106 10-11 0.12813025607524509 1106 12-13 0.13481654881635308 1106 14-15 -0.021838007603250276 1106 16-17 -0.05989638534798303 1106 18-19 0.05337029012334682 1106 20-21 0.15887587458958308 1106 22-23 0.10055545309074176 1106 24-25 0.17191529680955142 1106 26-27 0.015225293231615922 1106 28-29 0.08361260069386134 1106 30-31 0.21710583359515567 1106 32-33 0.013128816404922361 1106 34-35 0.02766700705945624 1106 36-37 0.016388641636375212 1106 38-39 0.02589537552165666 1106 40-41 0.1883402884038361 1106 42-43 0.09335242216744888 1106 44-45 -0.14545032073110065 1106 46-47 -0.0645766518525761 1106 48-49 -0.058928066651795064 1106 50-51 -0.10780046362807383 1106 52-53 0.014307698098171784 1106 54-55 0.16132120314075138 1106 56-57 0.043336424160990816 1106 58-59 -0.09432007723130909 1106 60-61 -0.08206252171714823 1106 62-63 -0.030906471987442785 1106 64-65 -0.02492834979479852 1106 66-67 -0.06529248246862096 1106 68-69 -0.0050299414280772226 1106 70-71 0.13792797979812121 1106 72-73 0.22024011077771988 1106 74-75 0.24316259690728614 1106 76-77 0.0399958565316183 1106 78-79 0.2542938505559462 1106 80-81 0.05618739452258481 1106 82-83 -0.06299947889605306 1106 84-85 -0.048234394202626873 1106 86-87 0.16234646683855303 1106 88-89 0.3657864044251369 1106 90-91 0.39316605276511396 1106 92-93 0.5998211479829187 1106 94-95 0.6516153303210253 1106 96-97 0.6963830853825819 1106 98-99 0.994063516626273 1106 100-101 1.0916927554350515 1107 1 -0.08836004772728501 1107 2 0.03255259897553486 1107 3 0.05006508365052298 1107 4 0.12044570765359452 1107 5 0.119312974793786 1107 6 0.07227261579055266 1107 7 0.06598387329439248 1107 8 0.004642941129333167 1107 9 0.043745653109240834 1107 10-11 0.05545758340257123 1107 12-13 0.03511684911665469 1107 14-15 0.0670508812856383 1107 16-17 0.09445796900637049 1107 18-19 0.022139058892115315 1107 20-21 -0.09517817946446883 1107 22-23 -0.1835286309933437 1107 24-25 -0.07102764613338053 1107 26-27 -0.012702634696317716 1107 28-29 -0.015786798705541116 1107 30-31 -0.04145272496339203 1107 32-33 -0.057141453865348524 1107 34-35 0.04178112117357102 1107 36-37 -0.009737484489754422 1107 38-39 -0.046777297151017194 1107 40-41 -0.02367172360817449 1107 42-43 0.17713620595122848 1107 44-45 0.06806319278241801 1107 46-47 0.04172965445372512 1107 48-49 -0.004273411997139931 1107 50-51 -0.10750016332777079 1107 52-53 -0.04124785745738535 1107 54-55 -0.1593995175799634 1107 56-57 0.019913000737567188 1107 58-59 0.0651393822281463 1107 60-61 0.22094048128585086 1107 62-63 0.3042286631476898 1107 64-65 0.14414071927426875 1107 66-67 0.1668396496635154 1107 68-69 0.22139648499834408 1107 70-71 0.3021922440623861 1107 72-73 0.336456326993936 1107 74-75 0.33595538970007865 1107 76-77 0.24870456524032747 1107 78-79 0.3873268835889796 1107 80-81 0.32105225938745363 1107 82-83 0.31507859918202286 1107 84-85 0.49020404423581354 1107 86-87 0.5296137341058227 1107 88-89 0.671492110130842 1107 90-91 0.6018747614738196 1107 92-93 0.6730944212561916 1107 94-95 0.7323961111018065 1107 96-97 0.7023890913885857 1107 98-99 0.647817270380024 1107 100-101 0.6010020647443604 1108 1 -0.004553628242476293 1108 2 0.004081414222945057 1108 3 0.02113514869044053 1108 4 0.07864511308918054 1108 5 0.0191017082508651 1108 6 6.634438044983426E-4 1108 7 -0.007943949000264183 1108 8 0.06128689664263476 1108 9 -0.0040085080871321566 1108 10-11 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1388.0 10 2375.0 11 3643.0 12 5236.0 13 7302.0 14 8880.0 15 11916.0 16 15892.0 17 22645.0 18 21726.0 19 13445.0 20 12083.0 21 12923.0 22 13327.0 23 14568.0 24 16488.0 25 19111.0 26 22748.0 27 26500.0 28 32099.0 29 39023.0 30 46393.0 31 55810.0 32 68362.0 33 87496.0 34 119275.0 35 170772.0 36 252709.0 37 354726.0 38 214932.0 39 10126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76094824764457 20.082855104237066 15.3044103829679 19.851786265150462 2 15.346593584577937 23.554606616911894 37.81059838843322 23.288201410076955 3 21.42811486796728 26.001980886613886 31.740863908163796 20.829040337255037 4 12.093377282666891 17.723481598806544 39.23961632489824 30.943524793628324 5 11.73830716878057 39.706162621525756 34.374070909642136 14.181459300051547 6 28.289969430544943 38.02915269304995 17.0285879820348 16.652289894370305 7 25.078798590509454 35.81511021247325 21.67469948050105 17.43139171651624 8 25.34520379734439 32.08438178217636 20.162235669211874 22.408178751267375 9 24.662390187750287 18.575180745641376 23.04630806246654 33.716121004141804 10-11 24.85830913911184 26.419444823980808 27.025908096947337 21.69633793996002 12-13 27.734640945242973 25.09451432800269 27.231649384242157 19.939195342512186 14-15 22.81142438946297 26.337440475370194 29.288536819287607 21.562598315879224 16-17 19.68630086417679 29.596962817205192 29.266105052911147 21.450631265706875 18-19 19.455036493824945 29.01327253423688 31.576064695211397 19.955626276726782 20-21 21.815466748607427 25.928005502853757 30.8047219927086 21.451805755830218 22-23 20.490301689383138 26.47233883599162 31.823224927974426 21.21413454665082 24-25 22.06279084018527 26.54086840616866 31.19677428529291 20.199566468353158 26-27 21.221470870314928 25.3570273556534 33.673880172744155 19.74762160128752 28-29 19.503523433161497 28.870689387244568 31.809239094421677 19.81654808517226 30-31 20.51390461602876 26.409945012511017 31.329322692762613 21.74682767869761 32-33 20.613066228346145 26.900475401059172 32.02301417289774 20.46344419769694 34-35 22.040046366822796 26.383930312530328 31.451555437999623 20.12446788264725 36-37 21.251534174141412 25.770723631633974 32.95680264178556 20.020939552439064 38-39 20.37848804766098 27.40937402591538 32.1352110825795 20.076926843844138 40-41 20.556597129166402 27.868062694586442 31.12459455354415 20.450745622703007 42-43 20.035444044561913 27.83087607896445 29.930278913667006 22.203400962806626 44-45 21.017301598099074 26.33303934377778 30.351890251196874 22.297768806926268 46-47 22.370532524357387 27.938932148232244 30.132526351190286 19.558008976220083 48-49 21.629077090883502 27.491995355546955 30.07553100406976 20.803396549499784 50-51 19.217300971710703 28.344303598534324 31.501857600298834 20.936537829456135 52-53 20.981447806452483 29.490991891734986 28.318791389417797 21.20876891239473 54-55 19.982794785893603 27.46671107730794 29.50606258030126 23.044431556497198 56-57 21.638019652881578 27.244843566839087 28.987971010328405 22.129165769950923 58-59 23.199020692991652 26.964280216679004 28.78001510009309 21.056683990236248 60-61 21.17452748035065 27.811763864202387 30.917336989443918 20.096371666003037 62-63 20.04675475772028 28.00726097341142 30.556225044435493 21.389759224432805 64-65 19.710729468515463 29.644057504605527 29.39166634169741 21.253546685181608 66-67 20.898555585984298 27.691365358576032 28.194954549426214 23.215124506013456 68-69 19.98335155631282 27.908192541391674 29.263905980567824 22.844549921727687 70-71 21.82294345207902 27.968090015991347 28.384732981996684 21.824233549932945 72-73 21.159950436786083 26.724934131708434 30.45182158884933 21.66329384265615 74-75 19.788165932385972 30.025737452185748 28.421412809616154 21.764683805812126 76-77 20.374351795603214 27.698423179525104 28.461976410789173 23.46524861408251 78-79 20.56383216101649 27.9973247191087 29.189267326651386 22.24957579322342 80-81 21.56751269869008 27.687747684563487 28.58858941625315 22.15615020049329 82-83 20.62291788468519 27.26557309371916 29.934756233078463 22.17675278851719 84-85 20.13433444985504 28.077794119026844 29.27987281973335 22.507998611384767 86-87 20.044673371096252 28.359813453017978 28.69778385214059 22.897729323745178 88-89 19.694872118811755 28.2114892079403 27.8146333102747 24.27900536297325 90-91 20.44041216673049 26.72772971272131 28.50384253905161 24.32801558149659 92-93 21.3198639298609 27.898747256343032 28.482135369967814 22.29925344382826 94-95 20.52981142166182 27.220302049040455 28.9179938145273 23.331892714770426 96-97 18.70498218198941 27.52315285841704 30.31941063638768 23.45245432320587 98-99 19.878971229058635 28.179988588498073 27.82899401101392 24.112046171429373 100-101 19.538853170014285 26.415651982182524 29.253406454730335 24.79208839307286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5373.0 1 3605.5 2 1949.5 3 1981.5 4 1656.5 5 1702.5 6 2803.5 7 5099.0 8 7416.0 9 8156.0 10 6716.5 11 5056.0 12 4736.0 13 4763.5 14 4907.0 15 4975.0 16 4949.0 17 5100.5 18 5289.0 19 5473.0 20 5820.0 21 6519.0 22 7514.5 23 8617.5 24 9619.0 25 10891.0 26 12868.0 27 15010.5 28 17074.0 29 19300.0 30 21887.0 31 24984.5 32 27231.5 33 29282.5 34 32236.0 35 34173.0 36 36640.5 37 39916.0 38 40680.5 39 40735.5 40 41996.0 41 43881.0 42 45533.5 43 46747.0 44 48140.0 45 49723.0 46 53681.0 47 79507.5 48 125575.0 49 122336.5 50 79133.0 51 55413.5 52 45833.5 53 43144.0 54 41212.0 55 37811.0 56 34113.0 57 30229.0 58 27820.5 59 25503.0 60 22534.0 61 18887.0 62 15255.0 63 11812.0 64 8552.5 65 6229.0 66 4270.5 67 3309.5 68 2365.0 69 1353.5 70 956.0 71 710.0 72 494.5 73 381.0 74 299.5 75 230.5 76 159.0 77 74.0 78 33.0 79 14.5 80 5.0 81 6.0 82 3.5 83 1.5 84 2.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39107557217305844 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.08932578899746E-4 16-17 0.0019058263751123704 18-19 4.984468981063122E-4 20-21 0.0 22-23 0.0 24-25 2.9320405770959544E-5 26-27 2.9320405770959544E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011640201091070938 36-37 0.022840596095577485 38-39 0.009998258367897205 40-41 0.0045739833002696895 42-43 0.004339420054102013 44-45 0.001231457042380301 46-47 7.916509558159076E-4 48-49 0.0021697100270510064 50-51 3.2252446348055496E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.570877096482314E-4 60-61 0.0014073794770060582 62-63 9.382529846707054E-4 64-65 4.3980608656439313E-4 66-67 0.0022283508385929256 68-69 0.0019058263751123704 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09101053951305843 78-79 0.0032545650405765097 80-81 1.7592243462575727E-4 82-83 0.0 84-85 5.864081154191909E-5 86-87 0.09367869643821575 88-89 0.4369620072046101 90-91 0.38793828875556574 92-93 0.0 94-95 6.450489269611099E-4 96-97 0.0 98-99 0.0 100-101 2.9320405770959544E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1705297.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.21318258571951 #Duplication Level Percentage of deduplicated Percentage of total 1 67.78315982261611 25.2242710271602 2 14.96182604171448 11.135543286121878 3 6.256045249515912 6.984220624042764 4 3.2191508753204925 4.79179397177121 5 1.9241364378607813 3.5801620290974596 6 1.2672172257782426 2.8294311599192814 7 0.8771098067980533 2.284803317167078 8 0.6269399966141733 1.8664346051434881 9 0.45387983454618097 1.5201281839448875 >10 2.3957304740730443 15.95790794120692 >50 0.15530082340561022 3.982697896132739 >100 0.07019655274815555 4.524259240118409 >500 0.0029971240875793143 0.7583037734825543 >1k 0.003943584325762256 4.073285248641775 >5k 0.001419690357274412 4.279855226880287 >10k+ 9.464602381829415E-4 6.206902469168977 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34634 2.0309658669428257 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18136 1.0635097581242445 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15152 0.888525576483158 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13498 0.7915336741928239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13012 0.7630342397834512 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11305 0.6629343744813952 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9311 0.5460045962668086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8959 0.5253630306040531 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8955 0.5251284673578854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8939 0.5241902143732148 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8056 0.4724103777817002 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8026 0.4706511534354426 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7964 0.4670154231198436 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6847 0.40151363662752004 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5852 0.3431660291433105 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4764 0.2793648261857025 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4676 0.27420443477001366 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4644 0.27232792880067225 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4637 0.2719174431198788 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4560 0.26740210063115105 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4515 0.2647632641117647 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4225 0.24775742876460816 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3386 0.19855778788093803 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3261 0.19122768643819815 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3098 0.18166923415686537 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2931 0.17187621862936484 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2880 0.168885537240727 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2658 0.15586727707842093 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2620 0.15363892623982803 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2153 0.12625366724975182 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.10080355504055892 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.9320405770959544E-5 0.0 16-17 0.0 0.0 0.0 1.7592243462575727E-4 0.0 18-19 0.0 0.0 0.0 2.638836519386359E-4 0.0 20-21 0.0 0.0 0.0 6.4504892696111E-4 0.0 22-23 0.0 0.0 0.0 0.0017005835347156536 0.0 24-25 0.0 0.0 0.0 0.002990681388637874 0.0 26-27 0.0 0.0 0.0 0.004691264923353527 0.0 28-29 0.0 0.0 0.0 0.013399425437328513 0.0 30-31 0.0 0.0 0.0 0.03386506866545828 0.0 32-33 0.0 0.0 0.0 0.07819752219114912 0.0 34-35 0.0 0.0 0.0 0.13622260521187804 0.0 36-37 0.0 0.0 0.0 0.21491857430113348 0.0 38-39 0.0 0.0 0.0 0.34181729047784637 0.0 40-41 0.0 0.0 0.0 0.48164630559955246 0.0 42-43 0.0 0.0 0.0 0.6239089144002482 0.0 44-45 0.0 0.0 0.0 0.7925305679890364 0.0 46-47 0.0 0.0 0.0 0.9959262228221829 0.0 48-49 0.0 0.0 0.0 1.189411580504745 0.0 50-51 0.0 0.0 0.0 1.4190196780971291 0.0 52-53 0.0 0.0 0.0 1.6419427231737345 0.0 54-55 0.0 0.0 0.0 1.8900813172133653 0.0 56-57 0.0 0.0 0.0 2.218440541442341 0.0 58-59 0.0 0.0 0.0 2.5392937417939514 0.0 60-61 0.0 0.0 0.0 2.8985566737055186 0.0 62-63 0.0 0.0 0.0 3.2534508651572134 0.0 64-65 0.0 0.0 0.0 3.618753800657598 0.0 66-67 0.0 0.0 0.0 4.045570947465456 0.0 68-69 0.0 0.0 0.0 4.544633574092959 0.0 70-71 0.0 0.0 0.0 5.079144571297551 0.0 72-73 0.0 0.0 0.0 5.599024686022434 0.0 74-75 0.0 0.0 0.0 6.166579780530899 0.0 76-77 0.0 0.0 0.0 6.718565739574983 0.0 78-79 0.0 0.0 0.0 7.433954319980625 0.0 80-81 0.0 0.0 0.0 8.19402719878121 0.0 82-83 0.0 0.0 0.0 8.905926650900106 0.0 84-85 0.0 0.0 0.0 9.648700490295825 0.0 86-87 0.0 0.0 0.0 10.396488119078377 0.0 88-89 0.0 0.0 0.0 11.224085892369482 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 415 0.0 34.30438 94-95 GTACATG 9010 0.0 25.85272 1 GACCGGT 130 1.00997695E-5 25.552362 7 TACATGG 9190 0.0 24.630844 2 GAGTACT 11445 0.0 24.049593 12-13 ACATGGG 9160 0.0 23.882612 3 TTTGCGT 720 0.0 23.727194 3 GTACTTT 12380 0.0 22.003569 14-15 AGTACTT 11845 0.0 21.955383 12-13 ATTCGCG 230 0.0 21.684626 80-81 CATGGGG 2555 0.0 21.544851 4 AGAGTAC 17050 0.0 21.27856 10-11 GGATACG 135 3.7251305E-4 21.170918 1 CTTTGCG 830 0.0 21.154367 2 CATGGGA 6510 0.0 20.774963 4 TACACCG 115 0.0037447892 20.632343 5 TCGTATC 105 8.6977343E-7 20.337597 94-95 CGCGGAA 240 0.0 19.896927 82-83 GGACCGA 120 0.004790976 19.772661 6 ACTTTTT 14055 0.0 19.498907 16-17 >>END_MODULE