##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939022_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1715566 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912565299149087 33.0 31.0 34.0 30.0 34.0 2 31.937993641748555 33.0 31.0 34.0 30.0 34.0 3 31.976355908195895 34.0 31.0 34.0 30.0 34.0 4 35.51877048157868 37.0 35.0 37.0 33.0 37.0 5 35.5790794408376 37.0 35.0 37.0 33.0 37.0 6 35.69602976510376 37.0 35.0 37.0 33.0 37.0 7 35.62854125110896 37.0 35.0 37.0 33.0 37.0 8 35.624796714320524 37.0 35.0 37.0 33.0 37.0 9 37.21579059039407 39.0 37.0 39.0 34.0 39.0 10-11 37.10839571313491 39.0 37.0 39.0 33.0 39.0 12-13 37.00752433890622 39.0 37.0 39.0 33.0 39.0 14-15 38.15048794392055 40.0 38.0 41.0 33.0 41.0 16-17 38.11610045897389 40.0 38.0 41.0 33.0 41.0 18-19 38.135288295524624 40.0 38.0 41.0 33.0 41.0 20-21 38.03471099333981 40.0 38.0 41.0 33.0 41.0 22-23 38.08518908628406 40.0 38.0 41.0 33.0 41.0 24-25 37.99707589215454 40.0 38.0 41.0 33.0 41.0 26-27 37.89031345923153 40.0 37.5 41.0 33.0 41.0 28-29 37.77666874955554 40.0 37.0 41.0 33.0 41.0 30-31 37.5730790887672 40.0 37.0 41.0 32.5 41.0 32-33 37.29635525535013 40.0 36.0 41.0 32.0 41.0 34-35 36.9024053869102 39.5 35.5 41.0 31.0 41.0 36-37 36.60343845704566 39.0 35.0 41.0 30.0 41.0 38-39 36.381177990237624 39.0 35.0 41.0 30.0 41.0 40-41 36.26884713266642 39.0 35.0 41.0 30.0 41.0 42-43 36.00302553209845 39.0 35.0 41.0 29.5 41.0 44-45 35.78161522203168 39.0 35.0 41.0 28.5 41.0 46-47 35.405451611887855 39.0 35.0 41.0 26.0 41.0 48-49 35.10067056586573 39.0 35.0 41.0 24.0 41.0 50-51 33.8661418447323 38.0 34.0 40.0 22.5 40.5 52-53 34.049153748675366 38.0 33.5 39.5 23.0 40.5 54-55 34.55654402104028 39.0 35.0 40.0 23.0 41.0 56-57 34.39078531516712 38.5 34.5 40.5 22.5 41.0 58-59 34.200279091565115 38.0 34.0 40.0 22.0 41.0 60-61 33.87564891120482 38.0 34.0 40.0 20.5 41.0 62-63 33.55954273983047 37.0 34.0 40.0 19.5 41.0 64-65 33.16621861239964 37.0 33.0 39.5 17.5 41.0 66-67 32.71602841278039 36.0 33.0 39.0 13.5 41.0 68-69 32.276952329435304 36.0 32.0 39.0 10.0 40.5 70-71 31.790610795504225 35.0 31.5 38.5 7.5 40.0 72-73 31.349164065970065 35.0 31.0 37.0 4.5 40.0 74-75 30.899645947751353 35.0 31.0 37.0 2.0 39.0 76-77 30.49897031067298 35.0 31.0 36.5 2.0 39.0 78-79 30.043774765879014 35.0 30.0 36.0 2.0 38.5 80-81 29.53786068271346 34.0 29.5 35.5 2.0 37.0 82-83 29.170277914111146 34.0 29.0 35.0 2.0 37.0 84-85 28.804697400158318 34.0 29.0 35.0 2.0 36.5 86-87 28.469025674325557 34.0 29.0 35.0 2.0 36.0 88-89 28.021437822852633 34.0 28.5 35.0 2.0 36.0 90-91 27.541287248639808 34.0 27.0 35.0 2.0 35.0 92-93 27.0963215638454 34.0 26.0 35.0 2.0 35.0 94-95 26.58855415647081 33.5 25.0 35.0 2.0 35.0 96-97 25.987545801210796 33.0 24.0 35.0 2.0 35.0 98-99 25.31989850579925 33.0 19.0 35.0 2.0 35.0 100-101 22.79625441399515 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2509998974713987 1101 2 0.30035417675265563 1101 3 0.3123327236845199 1101 4 0.21689288463981882 1101 5 -8.248895629421327E-4 1101 6 0.052345331873219436 1101 7 0.13918564599939032 1101 8 0.266336772596496 1101 9 0.25063824121067313 1101 10-11 0.3470211328420163 1101 12-13 0.41555302121094684 1101 14-15 0.1710165962588448 1101 16-17 0.41443373962358265 1101 18-19 0.4044952025228383 1101 20-21 0.45861149535152634 1101 22-23 0.39437938969559383 1101 24-25 0.5007259530068353 1101 26-27 0.32058240187379283 1101 28-29 0.44494862912949174 1101 30-31 0.2591091133764891 1101 32-33 0.37732268355293286 1101 34-35 0.32084574138247746 1101 36-37 0.28528574497238424 1101 38-39 0.39190700469924167 1101 40-41 0.39595219289966366 1101 42-43 0.31493951871466663 1101 44-45 0.43055142586121775 1101 46-47 0.4737066745764551 1101 48-49 0.5223743387897812 1101 50-51 0.49042427744196715 1101 52-53 0.47741502194614327 1101 54-55 0.3928824850486592 1101 56-57 0.3838056498409088 1101 58-59 0.23603282915784263 1101 60-61 0.22559096335717754 1101 62-63 0.26083143003396714 1101 64-65 0.2163788008108014 1101 66-67 0.33702619228904496 1101 68-69 0.3868440947384002 1101 70-71 0.422317666828647 1101 72-73 0.1804889197989219 1101 74-75 0.21867389892366873 1101 76-77 0.09257783939247233 1101 78-79 0.1337479865299933 1101 80-81 -0.008992135321573613 1101 82-83 -0.004789779721797061 1101 84-85 -0.2693520064340724 1101 86-87 -0.36830294122152907 1101 88-89 -0.44309409769167374 1101 90-91 -0.33233791843635885 1101 92-93 -0.4278723845616774 1101 94-95 -0.5130080484841955 1101 96-97 -0.6872426583167481 1101 98-99 -0.631820302416461 1101 100-101 -0.274628311744717 1102 1 0.1062650199782702 1102 2 0.06094438679884462 1102 3 0.038488395156413446 1102 4 -0.028573614413744508 1102 5 0.04292911412647271 1102 6 0.17342452585327806 1102 7 0.11019773051877735 1102 8 0.11655142654126394 1102 9 0.298300809062674 1102 10-11 0.058949959887449666 1102 12-13 0.13555605558607908 1102 14-15 0.26870420974687903 1102 16-17 0.2025211681592296 1102 18-19 0.26129758121606983 1102 20-21 0.5253958443977851 1102 22-23 0.3504658404881411 1102 24-25 0.20190040870576098 1102 26-27 0.31168939490436287 1102 28-29 0.3616094758967634 1102 30-31 0.22906779610446648 1102 32-33 0.2054375739863019 1102 34-35 0.27453198538798773 1102 36-37 0.42736173118444043 1102 38-39 0.37313140717619575 1102 40-41 0.36248516242907414 1102 42-43 0.29955170470098835 1102 44-45 0.20844580231317877 1102 46-47 0.34737492104478207 1102 48-49 0.34933387884915135 1102 50-51 -0.0015751670586681144 1102 52-53 0.17785075179363474 1102 54-55 0.2706178377666717 1102 56-57 0.27641345444641985 1102 58-59 0.07742291012267088 1102 60-61 0.008039110060380494 1102 62-63 0.143823958085882 1102 64-65 0.265251907738012 1102 66-67 0.30391658391609866 1102 68-69 0.3764818641997394 1102 70-71 0.37396975565074797 1102 72-73 0.25384092497501953 1102 74-75 0.2851590248662923 1102 76-77 0.11343615815598795 1102 78-79 -0.00100696760920016 1102 80-81 0.09161486011723596 1102 82-83 0.015555585149769513 1102 84-85 -0.04384675889167866 1102 86-87 -0.3055248905105401 1102 88-89 -0.19714755728070443 1102 90-91 -0.07215931575808199 1102 92-93 -0.0978618124133881 1102 94-95 -0.1400235398522831 1102 96-97 -0.1519389478699864 1102 98-99 -0.213594705455332 1102 100-101 -0.038056425150070794 1103 1 -0.018359383623714365 1103 2 0.09364522280750265 1103 3 0.12481170168202027 1103 4 0.08261226549821998 1103 5 0.039104222658060905 1103 6 0.09787026317490444 1103 7 0.07568491935205657 1103 8 0.021274166693203256 1103 9 0.23494710123110707 1103 10-11 0.1677391492534568 1103 12-13 0.18008438127898074 1103 14-15 0.1916824146414342 1103 16-17 0.2832691365507216 1103 18-19 0.23110889903176002 1103 20-21 0.2986602346660092 1103 22-23 0.3337392419545395 1103 24-25 0.2865799698752056 1103 26-27 0.25118916826143334 1103 28-29 0.046676082436853505 1103 30-31 0.12471664131592064 1103 32-33 0.18276841346858674 1103 34-35 0.13006035936906102 1103 36-37 -0.04607178455361094 1103 38-39 -0.009610863209594811 1103 40-41 -0.141964360063092 1103 42-43 -0.03622232373296441 1103 44-45 0.09321968120952562 1103 46-47 0.16542971722969924 1103 48-49 -0.14225015271574648 1103 50-51 -0.1830429409266543 1103 52-53 -0.05442339580309152 1103 54-55 -0.05146473814313168 1103 56-57 0.021092018698517734 1103 58-59 -0.0770062067175914 1103 60-61 -0.07583123460821639 1103 62-63 -0.020621452122234984 1103 64-65 -0.10998739136679347 1103 66-67 -0.033143506834534264 1103 68-69 -0.18084516674675655 1103 70-71 -0.18042201573118177 1103 72-73 -0.1270367984396117 1103 74-75 -0.04999601914605378 1103 76-77 -0.2743060810761335 1103 78-79 -0.28832146699313554 1103 80-81 -0.24195796703131478 1103 82-83 -0.005983670807204078 1103 84-85 -0.024068074542189066 1103 86-87 0.11435430335717456 1103 88-89 0.12360514646817577 1103 90-91 0.0020356734861159964 1103 92-93 -0.15891053830629076 1103 94-95 -0.15695244251108775 1103 96-97 -0.3038687617735576 1103 98-99 -0.27235675218894784 1103 100-101 -0.2828268927198856 1104 1 0.3150410074016712 1104 2 0.22836922558568062 1104 3 0.15174824113795182 1104 4 0.2797435221338702 1104 5 0.30028500917594414 1104 6 0.23961926036918868 1104 7 0.16609347662748775 1104 8 0.17927381789121455 1104 9 0.24438234797791836 1104 10-11 0.2891945255465487 1104 12-13 0.27525072005137474 1104 14-15 0.21123406640242592 1104 16-17 0.19115787483601565 1104 18-19 0.2893733643703271 1104 20-21 0.24238149292327904 1104 22-23 0.22643329365678966 1104 24-25 0.32929673960164507 1104 26-27 0.26643127967613367 1104 28-29 0.19524545884620892 1104 30-31 0.2907295592281258 1104 32-33 0.31158534749911837 1104 34-35 0.24497914052029302 1104 36-37 0.3448751975442761 1104 38-39 0.4015746657914505 1104 40-41 0.42454284698148115 1104 42-43 0.493098843346246 1104 44-45 0.4915079507539204 1104 46-47 0.42654701566651454 1104 48-49 0.37668574115053843 1104 50-51 0.3834512625350541 1104 52-53 0.4004040695997304 1104 54-55 0.4326265255909405 1104 56-57 0.3753891673952481 1104 58-59 0.3671479943575733 1104 60-61 0.3281679025658164 1104 62-63 0.463182967872072 1104 64-65 0.42242671614376803 1104 66-67 0.46003037707559713 1104 68-69 0.34583665546826126 1104 70-71 0.24596967360250943 1104 72-73 0.15071055265406486 1104 74-75 0.08878586640406283 1104 76-77 0.046618902019208264 1104 78-79 -0.10073369280876676 1104 80-81 -0.13104275957062939 1104 82-83 -0.2114356906460806 1104 84-85 -0.1399070504105957 1104 86-87 -0.047396116582063996 1104 88-89 0.0847022511057105 1104 90-91 0.16299440470358917 1104 92-93 -0.012389226198884273 1104 94-95 -0.010955863218303108 1104 96-97 0.11052406269642745 1104 98-99 0.18443540160213345 1104 100-101 0.4348156036008639 1105 1 0.059184040693903484 1105 2 0.014217780476833752 1105 3 0.07336881959397346 1105 4 -0.043892651329279886 1105 5 0.08422875165355492 1105 6 0.2011466164512541 1105 7 0.10996485685780044 1105 8 0.09015570331667533 1105 9 0.09330111281092712 1105 10-11 0.03327310752935375 1105 12-13 0.1873906950369033 1105 14-15 0.01069031832675904 1105 16-17 0.08356322716738873 1105 18-19 0.12431724385300669 1105 20-21 0.11439408265791684 1105 22-23 0.11796173988671654 1105 24-25 0.1695410947718159 1105 26-27 0.06718723829176554 1105 28-29 0.3152979921877943 1105 30-31 0.3297299672969842 1105 32-33 0.24915707259917497 1105 34-35 0.2709021937592837 1105 36-37 0.2771177200875101 1105 38-39 0.2187918571286076 1105 40-41 0.19938753774041373 1105 42-43 0.13185735644348995 1105 44-45 0.11194498219289528 1105 46-47 0.28878694542564176 1105 48-49 0.2859618682382177 1105 50-51 0.1925199668943165 1105 52-53 0.06793438381275507 1105 54-55 0.20547396234395165 1105 56-57 -0.0935114184855621 1105 58-59 0.13935717900063338 1105 60-61 0.16824100139950815 1105 62-63 -0.07574046691866698 1105 64-65 -0.013351052794966733 1105 66-67 -0.0784518424654479 1105 68-69 0.003398009431904825 1105 70-71 -0.02906873937790877 1105 72-73 0.15643898503617137 1105 74-75 -0.0605419248532435 1105 76-77 0.1221992952679507 1105 78-79 0.19827213653595166 1105 80-81 0.1181422075849845 1105 82-83 -0.001388504921713718 1105 84-85 0.13487871343685853 1105 86-87 0.17031193592770677 1105 88-89 0.11259972417242992 1105 90-91 -0.10109133689896055 1105 92-93 3.624967275328572E-5 1105 94-95 0.05929606405999621 1105 96-97 -0.02161069725742948 1105 98-99 0.12631984003603236 1105 100-101 0.6296581729909896 1106 1 0.004638586148448809 1106 2 0.02284817039754472 1106 3 0.16361537113085234 1106 4 0.03790815240700596 1106 5 0.12929446252572063 1106 6 0.0847091232395698 1106 7 0.04964987912347141 1106 8 -0.08633392414069618 1106 9 0.022313820533312878 1106 10-11 0.014164820356548091 1106 12-13 0.050609984707804756 1106 14-15 0.08508154239539323 1106 16-17 0.17736794097210407 1106 18-19 0.15372638906860914 1106 20-21 0.0760058997536035 1106 22-23 0.12871442805876399 1106 24-25 0.19008850435148616 1106 26-27 0.14133842760424642 1106 28-29 0.16341681482274595 1106 30-31 0.18807253273632085 1106 32-33 0.2696023859477208 1106 34-35 0.19109878331071428 1106 36-37 0.0967408329364261 1106 38-39 -0.05228775620907555 1106 40-41 0.1303531074156652 1106 42-43 -0.04275326590907014 1106 44-45 0.23345578918757326 1106 46-47 0.16350400852979874 1106 48-49 0.13763668088076741 1106 50-51 0.16777140601671903 1106 52-53 0.021924825867706943 1106 54-55 0.052380663766783186 1106 56-57 0.019301658521058584 1106 58-59 0.18722945009870529 1106 60-61 0.016162148352904637 1106 62-63 0.08639486489408199 1106 64-65 0.05515660442236481 1106 66-67 0.01481197854030114 1106 68-69 -0.033053603471323356 1106 70-71 -0.0918112179268391 1106 72-73 -0.21368917831779655 1106 74-75 -0.09714994049351944 1106 76-77 0.015786783048991282 1106 78-79 0.06205186934794327 1106 80-81 0.03060989521396351 1106 82-83 0.05939568102376214 1106 84-85 0.07685003960173376 1106 86-87 0.03025437103143247 1106 88-89 0.05628279140388415 1106 90-91 -0.10470054728236278 1106 92-93 -0.3322446235758605 1106 94-95 -0.2808527357662207 1106 96-97 -0.24783748122614924 1106 98-99 -0.3843057709121602 1106 100-101 0.07242237414228825 1107 1 0.06515418994763778 1107 2 0.005281458502473413 1107 3 0.10317213277716775 1107 4 0.10148119627856289 1107 5 0.11247854212771102 1107 6 0.06925910062536644 1107 7 0.08920664076978113 1107 8 0.23299669548843127 1107 9 0.16355307878749414 1107 10-11 0.09974148250037018 1107 12-13 0.13322870926682384 1107 14-15 0.004005106393591973 1107 16-17 0.1807329341878443 1107 18-19 0.11459025157425629 1107 20-21 0.06293489116020368 1107 22-23 0.18110248776025628 1107 24-25 0.21688181054777544 1107 26-27 0.31316113226277054 1107 28-29 0.3107483390199448 1107 30-31 0.414847747571244 1107 32-33 0.2371736206831372 1107 34-35 0.18182696060605252 1107 36-37 0.14254363709743956 1107 38-39 0.2930401497158428 1107 40-41 0.3358031300298663 1107 42-43 0.23952172278383443 1107 44-45 0.44363670280132084 1107 46-47 0.24477492666639478 1107 48-49 0.23676377269443094 1107 50-51 0.22918253220396423 1107 52-53 0.11829298507169028 1107 54-55 0.15485465743480376 1107 56-57 0.2137611790999827 1107 58-59 0.27346192409237347 1107 60-61 0.2804407553180823 1107 62-63 0.20693308289498447 1107 64-65 0.14474502595108873 1107 66-67 0.034585836523113755 1107 68-69 -0.09111330496386216 1107 70-71 0.09523536732870141 1107 72-73 0.07761339970119963 1107 74-75 0.10550044394790703 1107 76-77 0.1433082665406218 1107 78-79 0.2070722221294865 1107 80-81 0.12311554876439246 1107 82-83 0.18268378595365675 1107 84-85 0.22476048736292498 1107 86-87 0.19351081607892695 1107 88-89 0.34474954852735706 1107 90-91 0.43178068293021 1107 92-93 0.4543089720822131 1107 94-95 0.47742406206281274 1107 96-97 0.3121399045721347 1107 98-99 0.4848529807839057 1107 100-101 0.35296511498353667 1108 1 0.038163019672882825 1108 2 -0.02979720224782767 1108 3 0.05097545849093876 1108 4 0.17501980951866614 1108 5 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-1.2489562602154436 2316 60-61 -1.2441050333628425 2316 62-63 -1.2928608013775218 2316 64-65 -1.8091308866154918 2316 66-67 -1.675636494602518 2316 68-69 -1.8337151196470813 2316 70-71 -1.6545523911267281 2316 72-73 -1.5241711589156637 2316 74-75 -1.6336833089438372 2316 76-77 -1.813610375401769 2316 78-79 -1.5763116792232346 2316 80-81 -1.4486624516247772 2316 82-83 -1.4149705305853288 2316 84-85 -1.5613392409390698 2316 86-87 -1.791457581911807 2316 88-89 -1.9338370453021447 2316 90-91 -2.074982507395326 2316 92-93 -2.2261812374207324 2316 94-95 -2.2581035149242936 2316 96-97 -2.442756785324594 2316 98-99 -3.0887572875268816 2316 100-101 -3.5897847282676167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5404.0 3 1030.0 4 1048.0 5 1188.0 6 1420.0 7 1761.0 8 2363.0 9 2881.0 10 3687.0 11 5401.0 12 7915.0 13 12013.0 14 12022.0 15 12010.0 16 15997.0 17 22190.0 18 18834.0 19 11791.0 20 11280.0 21 11898.0 22 12989.0 23 13872.0 24 15651.0 25 17988.0 26 20626.0 27 23808.0 28 28291.0 29 33380.0 30 40217.0 31 48810.0 32 60813.0 33 77506.0 34 105739.0 35 156830.0 36 223029.0 37 328986.0 38 296147.0 39 48738.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.94050631653464 19.382300141055925 14.775389043701617 19.901804498707815 2 15.05934722428846 23.02701472814625 38.35262956772351 23.56100847984178 3 21.519587022576534 25.904088233784055 31.51508291753987 21.06124182609954 4 11.887952338021924 17.123826029111854 39.83083732315673 31.157384309709496 5 11.823131236505805 39.404213106144596 34.422419326603254 14.350236330746338 6 28.469460857065513 37.49789980678223 17.37116481382954 16.66147452232272 7 25.16765405677892 35.63507835135996 22.009619906344774 17.187647685516346 8 25.102449691134016 31.49552045143208 20.711873662494778 22.690156194939128 9 24.971819053820358 18.549830680940346 23.228859622713728 33.24949064252557 10-11 24.81243912421126 26.11249364441451 27.23306030447885 21.84200692689538 12-13 27.72449342753957 24.982730152803143 27.332333704790635 19.960442714866655 14-15 23.048132351787068 26.111511287752876 29.380366098902076 21.459990261557984 16-17 19.79851372145769 29.460170214627958 29.226828859182763 21.514487204731587 18-19 19.49090558997332 29.00262716055146 31.557522562801683 19.948944686673542 20-21 21.926533155832367 25.898802860437968 30.640885264181478 21.533778719548184 22-23 20.532550490667514 26.426921586569435 31.715661444084446 21.324866478678604 24-25 22.173192619898632 26.388730007167187 31.037012567090237 20.40106480584394 26-27 21.242123135459746 25.25913800917456 33.63936603953308 19.85937281583261 28-29 19.60308838081198 28.806659295592617 31.65485281342458 19.93539951017083 30-31 20.49258249245294 26.364029479785316 31.271792812828807 21.87159521493294 32-33 20.64400813690352 26.936716084856275 31.866679426775452 20.552596351464747 34-35 22.11933650708946 26.394779217982745 31.207197592064674 20.27868668286312 36-37 21.240742961191163 25.72528189975354 32.88321817339103 20.150756965664268 38-39 20.438304303374792 27.402416661976854 31.935709662896056 20.223569371752305 40-41 20.534376665550315 27.88367466951233 31.10936675465607 20.47258191028128 42-43 20.035322764249564 27.86708769494637 29.722763780626053 22.37482576017801 44-45 20.949390020825128 26.316755314159206 30.319582418716344 22.41427224629932 46-47 22.39679496405092 27.906476403808036 30.125180595439545 19.571548036701508 48-49 21.600844821262662 27.437018803547097 30.091978683625637 20.8701576915646 50-51 19.21952901217793 28.283249359237633 31.62764341043002 20.869578218154412 52-53 21.00053473176635 29.383070814434003 28.416859764248958 21.199534689550685 54-55 19.934525664292185 27.403659422063527 29.79618709314879 22.865627820495497 56-57 21.589433162502168 27.124527010235806 29.13086519029779 22.155174636964237 58-59 23.18534036507257 26.913369476637104 28.867874158944385 21.03341599934594 60-61 21.26446712817767 27.579861359251055 31.13357029802958 20.022101214541696 62-63 20.07887447790723 27.830526855719206 30.793844801055176 21.296753865318387 64-65 19.748516481212768 29.46548757257067 29.618215461551067 21.167780484665492 66-67 20.863263151721306 27.608351416339218 28.402850272711277 23.125535159228196 68-69 19.951532696369117 27.720934133380098 29.627222859864155 22.70031031038663 70-71 21.875674433007102 27.73734763678674 28.658007337831116 21.728970592375035 72-73 21.092362539879687 26.544763325554577 30.860462954736906 21.502411179828833 74-75 19.77834489625622 29.81427977484406 28.837388223938564 21.56998710496116 76-77 20.345143283976753 27.48819097768122 28.84016389718111 23.32650184116092 78-79 20.5672426471558 27.751196592945032 29.558476180539213 22.123084579359954 80-81 21.579315246140656 27.52531742949878 28.918973968216754 21.97639335614381 82-83 20.649175238271663 26.958435385555152 30.419603171718567 21.972786204454618 84-85 20.27724824854726 27.78292568458285 29.71880898575357 22.22101708111632 86-87 20.12906330908218 28.14390301023637 29.244071579252207 22.482962101429248 88-89 19.895580339671703 27.928301626830166 28.304059966551705 23.872058066946426 90-91 20.521589884260536 26.48658775570517 29.0451933936209 23.946628966413392 92-93 21.512771952108807 27.404609496431735 29.229044231590795 21.853574319868663 94-95 20.80537207929693 26.74469575487396 29.440792511881607 23.009139653947503 96-97 18.996720846199945 26.971157285620627 31.056993475703653 22.97512839247577 98-99 20.28863377335924 27.67741559035221 28.549697242119223 23.484253394169325 100-101 19.832924247794146 25.89996620024173 29.885611416520607 24.381498135443515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7364.0 1 4655.0 2 2151.0 3 2271.5 4 1905.0 5 1932.0 6 3047.5 7 5401.5 8 8044.0 9 8799.0 10 7070.0 11 5413.5 12 5163.5 13 5192.5 14 5375.0 15 5566.0 16 5752.5 17 5853.5 18 5793.5 19 5829.0 20 6105.0 21 6698.0 22 7642.5 23 8849.5 24 9839.0 25 10929.5 26 12941.5 27 15250.0 28 16997.0 29 19114.5 30 21982.5 31 25089.0 32 27288.0 33 29304.5 34 32109.0 35 33819.0 36 36232.5 37 39857.0 38 41055.5 39 40837.5 40 41780.0 41 43539.0 42 44973.0 43 46398.5 44 47904.0 45 49497.0 46 54798.5 47 79148.5 48 125534.0 49 123559.5 50 77106.5 51 53289.5 52 45692.5 53 43458.0 54 41493.0 55 38095.0 56 34203.0 57 30223.5 58 27799.0 59 25568.0 60 22637.0 61 19007.5 62 15417.5 63 12067.5 64 8801.0 65 6472.0 66 4516.0 67 3494.5 68 2587.0 69 1572.5 70 1090.5 71 803.0 72 612.5 73 455.0 74 328.5 75 269.0 76 203.0 77 107.5 78 60.5 79 43.0 80 30.5 81 24.0 82 17.5 83 11.0 84 7.0 85 8.0 86 4.5 87 3.5 88 4.0 89 0.5 90 0.5 91 2.5 92 2.5 93 0.5 94 0.5 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28544515337795223 2 0.08749299065148178 3 0.09174814609289296 4 0.10672862483868298 5 0.10876876785853765 6 0.08382073321574338 7 0.09442947691898766 8 0.09058235008154743 9 0.09571185253146774 10-11 0.08874622136367823 12-13 0.09303052170537303 14-15 0.10139510808677718 16-17 0.0905532051812638 18-19 0.10133681828620991 20-21 0.10331867150549731 22-23 0.1155303847243417 24-25 0.12835414084914248 26-27 0.13065658797154991 28-29 0.123078913897804 30-31 0.11570525412604353 32-33 0.11028430267328683 34-35 0.38179819371566004 36-37 0.5852004527951709 38-39 0.5678592371264061 40-41 0.531049228068171 42-43 0.5586494486367765 44-45 0.5790508788353231 46-47 0.5683547004312279 48-49 0.5896887674388511 50-51 0.5817613545617015 52-53 0.5851130180943199 54-55 0.574708288693061 56-57 0.547807545731263 58-59 0.5422408697770881 60-61 0.4908001207764668 62-63 0.564886457297475 64-65 0.5972081517120298 66-67 0.6100610527371142 68-69 0.6119846161558343 70-71 0.6079626199166922 72-73 0.5992482947318845 74-75 0.5979950640196879 76-77 0.5819945137639706 78-79 0.5582122751325218 80-81 0.5524998746769287 82-83 0.5677426575252715 84-85 0.5776810685219922 86-87 0.5675386432232861 88-89 0.5798960809435486 90-91 0.5936233289771422 92-93 0.6018713357574118 94-95 0.600647249945499 96-97 0.5973538762134479 98-99 0.6018713357574118 100-101 0.5789634441344722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1715566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.70534991749113 #Duplication Level Percentage of deduplicated Percentage of total 1 69.45251277175592 26.881838094798344 2 14.277325272616482 11.052177411249206 3 5.90303784675707 6.854374363047773 4 3.0428711826700967 4.711015755163846 5 1.838248305618532 3.5575021952100223 6 1.1470925508832173 2.6639171141809452 7 0.8269975720781824 2.2406461285741144 8 0.6021631621535454 1.864554871886074 9 0.41993291705256525 1.4628285446753084 >10 2.2717176168640574 15.589549891459207 >50 0.14534105389240815 3.873988110877781 >100 0.06430642972110383 4.293042257805017 >500 0.0024152336962614323 0.6149977688970647 >1k 0.0036228505443921485 3.730359353103828 >5k 0.001509521060163395 4.348629008288013 >10k+ 9.057126360980371E-4 6.260579130783504 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 33228 1.936853493249458 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19474 1.1351355762471393 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15711 0.9157910567124786 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14703 0.8570349377406641 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12621 0.7356755729595946 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11416 0.6654363632760267 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9145 0.5330602261877421 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8474 0.4939477700070997 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8183 0.47698543804202226 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8174 0.4764608298369168 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7962 0.46410339211665425 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7618 0.4440517007215112 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7481 0.4360659980437943 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6918 0.40324884032441777 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5461 0.3183206008978961 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5013 0.2922067702437563 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4918 0.28666923918986503 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4663 0.27180534004520956 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4603 0.26830795201117297 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4468 0.26043882893459064 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4420 0.25764091850736137 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4410 0.25705802050168863 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3463 0.20185757936447796 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3138 0.18291339418011315 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3071 0.17900797754210562 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2729 0.15907286574809715 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2707 0.15779049013561705 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2572 0.14992136705903475 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2526 0.14724003623294002 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2245 0.13086060227353538 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.1130822131005161 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1772 0.10328952660521368 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.828980056727634E-5 0.0 8 0.0 0.0 0.0 5.828980056727634E-5 0.0 9 0.0 0.0 0.0 5.828980056727634E-5 0.0 10-11 0.0 0.0 0.0 8.743470085091451E-5 0.0 12-13 0.0 0.0 0.0 2.040143019854672E-4 0.0 14-15 0.0 0.0 0.0 3.2059390312001983E-4 0.0 16-17 0.0 0.0 0.0 3.788837036872962E-4 0.0 18-19 0.0 0.0 0.0 5.24608205105487E-4 0.0 20-21 0.0 0.0 0.0 7.869123076582306E-4 0.0 22-23 0.0 0.0 0.0 0.0015738246153164611 0.0 24-25 0.0 0.0 0.0 0.002739620626661988 0.0 26-27 0.0 0.0 0.0 0.004255155441411173 0.0 28-29 0.0 0.0 0.0 0.012532307121964414 0.0 30-31 0.0 0.0 0.0 0.03748034176475869 0.0 32-33 0.0 0.0 0.0 0.07976959207631767 0.0 34-35 0.0 0.0 0.0 0.1388171600509686 0.0 36-37 0.0 0.0 0.0 0.22071432984799186 0.0 38-39 0.0 0.0 0.0 0.35233269952890184 0.0 40-41 0.0 0.0 0.0 0.5023706461890711 0.0 42-43 0.0 0.0 0.0 0.6500187110259821 0.0 44-45 0.0 0.0 0.0 0.8287060946649678 0.0 46-47 0.0 0.0 0.0 1.0398317523196425 0.0 48-49 0.0 0.0 0.0 1.2464982402309208 0.0 50-51 0.0 0.0 0.0 1.4772967055770514 0.0 52-53 0.0 0.0 0.0 1.7079203015214803 0.0 54-55 0.0 0.0 0.0 1.9601694134763687 0.0 56-57 0.0 0.0 0.0 2.285397355741487 0.0 58-59 0.0 0.0 0.0 2.6067781711691653 0.0 60-61 0.0 0.0 0.0 2.9718763370223007 0.0 62-63 0.0 0.0 0.0 3.3334479699411155 0.0 64-65 0.0 0.0 0.0 3.696943166278651 2.914490028363817E-5 66-67 0.0 0.0 0.0 4.126130967855507 5.828980056727634E-5 68-69 0.0 0.0 0.0 4.6273941078337995 5.828980056727634E-5 70-71 0.0 0.0 0.0 5.161066376927498 5.828980056727634E-5 72-73 0.0 0.0 0.0 5.681914889896396 5.828980056727634E-5 74-75 0.0 0.0 0.0 6.247646549302097 5.828980056727634E-5 76-77 0.0 0.0 0.0 6.80859844506128 5.828980056727634E-5 78-79 0.0 0.0 0.0 7.524892659332256 5.828980056727634E-5 80-81 0.0 0.0 0.0 8.294084867618034 5.828980056727634E-5 82-83 0.0 0.0 0.0 9.003413450721219 5.828980056727634E-5 84-85 0.0 0.0 0.0 9.748153087669026 5.828980056727634E-5 86-87 0.0 0.0 0.0 10.497147880058243 5.828980056727634E-5 88-89 0.0 0.0 0.0 11.33185782418164 5.828980056727634E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 80 4.5868978E-4 29.58457 3 CGTCACT 160 6.352457E-8 26.672031 1 TTTGCGT 850 0.0 25.616758 3 GTACATG 9410 0.0 24.036007 1 GTAAGCG 320 0.0 23.783552 94-95 GAGTACT 10880 0.0 23.666964 12-13 CTTTGCG 940 0.0 23.16409 2 TACATGG 9530 0.0 23.046783 2 AGTACTT 11165 0.0 22.257334 12-13 ACATGGG 9490 0.0 22.146341 3 GTACTTT 11865 0.0 21.821562 14-15 CATGGGG 2850 0.0 21.591547 4 AGAGTAC 16650 0.0 20.710371 10-11 TTGCGTT 1075 0.0 20.695438 4 GTACGGA 140 4.910679E-4 20.321548 1 TATCACG 120 0.004861116 19.72305 2 TACTTTT 12695 0.0 19.66781 14-15 ACTTTTT 13510 0.0 19.306646 16-17 GCTTTGC 1180 0.0 18.88641 1 ATGGGAG 2145 0.0 18.536907 5 >>END_MODULE