##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939022_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1715566 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09229956760626 33.0 31.0 34.0 30.0 34.0 2 32.30134544517669 34.0 31.0 34.0 30.0 34.0 3 32.384146689780515 34.0 31.0 34.0 30.0 34.0 4 35.96766548182932 37.0 35.0 37.0 35.0 37.0 5 35.90206205998487 37.0 35.0 37.0 35.0 37.0 6 35.971688643864475 37.0 35.0 37.0 35.0 37.0 7 35.91827128772662 37.0 35.0 37.0 35.0 37.0 8 35.90151471875754 37.0 35.0 37.0 35.0 37.0 9 37.53161289043965 39.0 37.0 39.0 35.0 39.0 10-11 37.45499677657403 39.0 37.0 39.0 34.0 39.0 12-13 37.39595620337545 39.0 37.0 39.0 34.0 39.0 14-15 38.52866138638793 40.0 38.0 41.0 34.0 41.0 16-17 38.56189211024234 40.0 38.0 41.0 34.0 41.0 18-19 38.72429274070482 40.0 38.0 41.0 34.0 41.0 20-21 38.729065218126266 40.0 38.0 41.0 34.0 41.0 22-23 38.65729648407581 40.0 38.0 41.0 34.0 41.0 24-25 38.564099544989816 40.0 38.0 41.0 34.0 41.0 26-27 38.36677807790549 40.0 38.0 41.0 34.0 41.0 28-29 38.192540537641804 40.0 38.0 41.0 33.5 41.0 30-31 37.99774214457503 40.0 37.0 41.0 33.0 41.0 32-33 37.73440776979726 40.0 36.5 41.0 33.0 41.0 34-35 37.596795751373016 40.0 36.0 41.0 33.0 41.0 36-37 37.44027073280772 40.0 36.0 41.0 32.5 41.0 38-39 37.2711367560327 40.0 36.0 41.0 32.0 41.0 40-41 37.14582971450821 40.0 36.0 41.0 32.0 41.0 42-43 36.8961873224347 40.0 35.0 41.0 31.0 41.0 44-45 36.55142938248951 39.5 35.0 41.0 30.5 41.0 46-47 36.10774257591955 39.0 35.0 41.0 29.5 41.0 48-49 35.67706721863222 39.0 35.0 41.0 27.5 41.0 50-51 35.35895383797534 39.0 35.0 40.5 27.0 41.0 52-53 35.17452636622549 39.0 35.0 40.0 26.0 41.0 54-55 34.947155341152715 38.5 35.0 40.0 26.0 41.0 56-57 34.6137904341774 38.0 34.0 40.0 24.5 41.0 58-59 34.31095743329024 38.0 34.0 40.0 24.0 41.0 60-61 33.81205881907196 37.0 33.0 40.0 22.0 41.0 62-63 33.55997262710965 37.0 33.0 40.0 22.0 41.0 64-65 33.335020045862414 36.5 33.0 39.5 22.0 41.0 66-67 32.841349152408014 36.0 32.5 39.0 20.5 40.0 68-69 32.389120849911926 35.5 32.0 39.0 19.0 40.0 70-71 31.87716269732555 35.0 31.5 37.5 17.5 40.0 72-73 31.348082790169542 35.0 31.0 37.0 12.5 39.0 74-75 30.918307427402965 35.0 31.0 36.5 9.0 39.0 76-77 28.973956991453548 32.5 28.0 35.0 7.0 36.5 78-79 29.9417693635803 34.0 30.0 35.0 4.5 37.0 80-81 29.90547551070609 34.0 30.0 35.0 2.0 37.0 82-83 29.57337957268913 34.0 30.0 35.0 2.0 37.0 84-85 29.167653998738608 34.0 29.0 35.0 2.0 36.0 86-87 28.68009974550673 34.0 29.0 35.0 2.0 36.0 88-89 28.111178176764987 34.0 28.5 35.0 2.0 35.0 90-91 27.63189553768261 33.0 27.0 35.0 2.0 35.0 92-93 27.3002568831511 33.0 26.0 35.0 2.0 35.0 94-95 26.89546044862162 33.0 25.5 35.0 2.0 35.0 96-97 26.274971933461025 33.0 25.0 34.5 2.0 35.0 98-99 25.475510997536674 32.5 22.0 34.0 2.0 35.0 100-101 21.353402608818314 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17983827400860264 1101 2 0.17491373426993562 1101 3 0.1852222692911596 1101 4 0.12478509235784685 1101 5 0.10657821440430126 1101 6 0.13494147337577544 1101 7 0.16835051010544078 1101 8 0.1508482654327281 1101 9 0.16147989648025884 1101 10-11 0.27511709454157085 1101 12-13 0.20931876740185373 1101 14-15 0.30503492893397066 1101 16-17 0.20063658057662082 1101 18-19 0.2611385113128506 1101 20-21 0.28671607723098447 1101 22-23 0.34522186990022163 1101 24-25 0.2590481926502335 1101 26-27 0.30207654983361465 1101 28-29 0.4035191268395053 1101 30-31 0.25946518554706444 1101 32-33 0.3652531080217045 1101 34-35 0.6013774177130529 1101 36-37 0.46694120019480323 1101 38-39 0.5166504662697875 1101 40-41 0.3560427147693588 1101 42-43 0.38783391425680946 1101 44-45 0.3252902472118606 1101 46-47 0.514804686273699 1101 48-49 0.1076295174370685 1101 50-51 0.08040489690015562 1101 52-53 0.10829843130142791 1101 54-55 0.24038056748857883 1101 56-57 -0.0861018115215586 1101 58-59 0.01882961034004893 1101 60-61 0.1608774031065181 1101 62-63 -0.01165318067710075 1101 64-65 0.05410864527228654 1101 66-67 0.018151456007949207 1101 68-69 0.014137315194062694 1101 70-71 -0.03563193705263501 1101 72-73 0.010092422480344254 1101 74-75 0.05448412593677432 1101 76-77 -0.07581523944664781 1101 78-79 0.17902346893088605 1101 80-81 0.2561845471437785 1101 82-83 0.28696854699658303 1101 84-85 0.0945806816367174 1101 86-87 0.2135644264120664 1101 88-89 0.29476310910006376 1101 90-91 0.4486598689000445 1101 92-93 0.45838806445957303 1101 94-95 0.24382339352526117 1101 96-97 0.13521950415192663 1101 98-99 0.4174254939410922 1101 100-101 0.845623890716098 1102 1 0.0984800941199282 1102 2 0.028939418890786328 1102 3 0.1562042508826167 1102 4 0.09338372480189605 1102 5 0.05144048748843488 1102 6 0.11314173571275887 1102 7 0.12501032133198464 1102 8 0.04082808442984032 1102 9 0.11069705980509781 1102 10-11 0.18151866278370932 1102 12-13 0.13817109929505733 1102 14-15 0.2869844106016828 1102 16-17 0.24285132121782738 1102 18-19 0.041496712072884634 1102 20-21 0.03738937938571496 1102 22-23 0.0994117960958647 1102 24-25 0.1274865571059749 1102 26-27 0.08969015828362359 1102 28-29 0.17474931710606967 1102 30-31 0.07402332272970824 1102 32-33 0.0550617015647461 1102 34-35 0.24182443415166688 1102 36-37 0.3067568980288371 1102 38-39 0.3129565961892098 1102 40-41 0.297597478818588 1102 42-43 0.14088374222860978 1102 44-45 0.00601297436052306 1102 46-47 0.09083324311524876 1102 48-49 -0.060300669457966194 1102 50-51 0.05824753645331526 1102 52-53 0.1065580806946329 1102 54-55 0.14727642580734113 1102 56-57 0.1978404438379684 1102 58-59 -0.12657274940839613 1102 60-61 -0.08347348143791322 1102 62-63 -0.11947123215873034 1102 64-65 -0.12296119191502441 1102 66-67 -0.13408667661511942 1102 68-69 -0.05940132813520904 1102 70-71 0.15909366873868436 1102 72-73 0.2424533108297915 1102 74-75 0.2713580612965565 1102 76-77 0.05913397892079075 1102 78-79 0.18518634102314735 1102 80-81 0.2368821483819339 1102 82-83 0.3577367175252526 1102 84-85 0.27536361464124326 1102 86-87 0.4030515206848051 1102 88-89 0.5345684515268907 1102 90-91 0.5546926160691612 1102 92-93 0.5547914870420634 1102 94-95 0.5315816967155307 1102 96-97 0.43499750509274193 1102 98-99 0.33248847729377573 1102 100-101 1.0049598923729661 1103 1 -0.01323890291892127 1103 2 0.029094018963377266 1103 3 0.03229949698879864 1103 4 0.03297209139955726 1103 5 0.02011352819537393 1103 6 0.0045638071512357214 1103 7 0.03371450012911481 1103 8 0.07470419252683058 1103 9 -0.005758462883335369 1103 10-11 0.017621362404725005 1103 12-13 -0.02942375193838842 1103 14-15 0.077491187303103 1103 16-17 0.05331280048739018 1103 18-19 0.09355507379499528 1103 20-21 0.029923987215561 1103 22-23 0.10900595727559192 1103 24-25 0.19379986330446997 1103 26-27 0.040815896041401345 1103 28-29 0.14472399696320082 1103 30-31 0.26664995070353115 1103 32-33 0.028191079628513194 1103 34-35 -0.05290424856225684 1103 36-37 -0.21573682840146802 1103 38-39 0.02521237091081474 1103 40-41 0.1877262836548823 1103 42-43 0.1753371667175756 1103 44-45 0.2704704176725343 1103 46-47 0.3687652315447778 1103 48-49 0.4372989404981169 1103 50-51 0.4743310454154539 1103 52-53 0.48484771322155495 1103 54-55 0.5073734850569522 1103 56-57 0.4183873776161704 1103 58-59 0.212847621039856 1103 60-61 0.3089897066102978 1103 62-63 0.5504521609740749 1103 64-65 0.33938356546800463 1103 66-67 0.37181927981024643 1103 68-69 0.5084994521083814 1103 70-71 0.3954880835092638 1103 72-73 0.2086408730397693 1103 74-75 0.1871218557508918 1103 76-77 0.0885395358003791 1103 78-79 0.2189874856088707 1103 80-81 0.3558478777905272 1103 82-83 0.3020861432852868 1103 84-85 0.23163223869280714 1103 86-87 0.07889430526187269 1103 88-89 0.14764399605404677 1103 90-91 0.17513347161116855 1103 92-93 0.16345333579412724 1103 94-95 0.024266426278334308 1103 96-97 0.10331921031986013 1103 98-99 0.1622461484948552 1103 100-101 0.8081777546394875 1104 1 0.03914335346278364 1104 2 0.048480831188577156 1104 3 0.008001919938742219 1104 4 0.026113848810837226 1104 5 0.06917451652886086 1104 6 0.0076578949575463184 1104 7 0.022184146707026287 1104 8 0.05249789756885548 1104 9 0.0981049780788652 1104 10-11 0.03177270269165433 1104 12-13 -0.047263520260436565 1104 14-15 0.043623738177707594 1104 16-17 -0.04689969335503008 1104 18-19 0.014665778650382322 1104 20-21 0.11664526383083995 1104 22-23 -0.0996923687808291 1104 24-25 0.0653642402186776 1104 26-27 0.027623378761937545 1104 28-29 0.12678631931130724 1104 30-31 0.07616199508093047 1104 32-33 -0.01684912439004904 1104 34-35 0.11861798144511226 1104 36-37 0.19268276203157342 1104 38-39 0.2777671924863583 1104 40-41 0.14290675808678088 1104 42-43 0.09080355169093934 1104 44-45 0.14019937711053387 1104 46-47 0.11882871962584574 1104 48-49 0.07339673356063514 1104 50-51 0.004515038463885901 1104 52-53 0.001920652612618312 1104 54-55 0.07106503395194608 1104 56-57 0.00430510930177519 1104 58-59 -0.03650449456658578 1104 60-61 0.10199829203465782 1104 62-63 0.12366845186268449 1104 64-65 0.1088900871472731 1104 66-67 0.09180701054735607 1104 68-69 -0.02580973637626016 1104 70-71 -0.16208336735402185 1104 72-73 -0.2215340939923216 1104 74-75 0.01839310131055072 1104 76-77 0.0012181820815087008 1104 78-79 0.11098087366944043 1104 80-81 0.306432733052997 1104 82-83 0.3304133011916157 1104 84-85 0.16515677568876796 1104 86-87 0.033974958072327865 1104 88-89 0.16006509490269494 1104 90-91 0.08110150421497053 1104 92-93 0.14651463927327413 1104 94-95 0.19006134294831512 1104 96-97 0.0036490423747608247 1104 98-99 -0.03332008869661607 1104 100-101 0.39411001974123394 1105 1 -0.009907407651937206 1105 2 0.03922635974087996 1105 3 0.012976824440322332 1105 4 0.03072046011566698 1105 5 0.12021921701074234 1105 6 0.04764348732769008 1105 7 0.05034900063458281 1105 8 0.039987714261968677 1105 9 -0.1421429863646324 1105 10-11 4.23954078286215E-4 1105 12-13 -0.022496539366017032 1105 14-15 0.021705873453278457 1105 16-17 0.0971736588643779 1105 18-19 0.027878665376647405 1105 20-21 0.21475304704463127 1105 22-23 0.06411118657437243 1105 24-25 0.01906868050877364 1105 26-27 0.027811576585470732 1105 28-29 0.031051080870931003 1105 30-31 -0.07440326131742836 1105 32-33 -0.07332762269663817 1105 34-35 0.03347338217021445 1105 36-37 -0.0068176114177376235 1105 38-39 0.18930574468161154 1105 40-41 0.2170434420043037 1105 42-43 0.16673271859699668 1105 44-45 0.11930094795467738 1105 46-47 0.16896741884374222 1105 48-49 0.09330482231045778 1105 50-51 0.18606480150404536 1105 52-53 0.3087379806602044 1105 54-55 0.3328261088966684 1105 56-57 0.3595003267936363 1105 58-59 0.2616023310848874 1105 60-61 0.42082455634837146 1105 62-63 0.13031614406385472 1105 64-65 0.06630434480813818 1105 66-67 0.3072342232897043 1105 68-69 0.11612972506123498 1105 70-71 0.13744654320966276 1105 72-73 0.1111429408644149 1105 74-75 0.24930593809626345 1105 76-77 0.165577491870593 1105 78-79 0.20581084743223244 1105 80-81 0.2823481994521373 1105 82-83 0.3613867590375186 1105 84-85 0.16090114780042697 1105 86-87 0.007014422898116379 1105 88-89 0.10625303934051189 1105 90-91 0.11006592267264992 1105 92-93 0.2536909058704069 1105 94-95 -0.011575867628476288 1105 96-97 -0.1702310148432673 1105 98-99 0.03787798380411189 1105 100-101 0.5989598518731967 1106 1 0.012253991283657228 1106 2 -0.11363776538131276 1106 3 -0.03361598537829735 1106 4 -0.07761667467308087 1106 5 -0.09976992168807897 1106 6 -0.08812060521059806 1106 7 -0.012872461296559834 1106 8 -0.10533866783860901 1106 9 -0.006381066086582621 1106 10-11 0.04630209027899923 1106 12-13 0.04090522016478104 1106 14-15 -0.057930272957065654 1106 16-17 -0.11721430681390643 1106 18-19 -0.09531890169188273 1106 20-21 -0.060854642756616784 1106 22-23 -0.02701404422828091 1106 24-25 0.047299374545922035 1106 26-27 -0.10294980062752046 1106 28-29 -0.0802218636278269 1106 30-31 -0.02231463309976789 1106 32-33 0.032973018120131314 1106 34-35 -0.06836217772986686 1106 36-37 -0.19826107834506956 1106 38-39 -0.08494102408128867 1106 40-41 -0.02624318773393952 1106 42-43 -0.0017727663600979326 1106 44-45 -0.08646205845349186 1106 46-47 -0.11911881988766737 1106 48-49 -0.31794205506545126 1106 50-51 -0.2383101306128239 1106 52-53 -0.17046697209313066 1106 54-55 -0.1633724356889772 1106 56-57 -0.30575654297935984 1106 58-59 -0.11782310608248281 1106 60-61 -0.08986622240692554 1106 62-63 -0.1129563659828392 1106 64-65 -0.25968892118512343 1106 66-67 -0.14738291584355778 1106 68-69 -0.29584485949399664 1106 70-71 -0.3704800343943404 1106 72-73 -0.2090721128990225 1106 74-75 -0.21340938719970737 1106 76-77 -0.3483173707339482 1106 78-79 -0.12241224369408599 1106 80-81 -0.08504617439384887 1106 82-83 -0.24355187461079453 1106 84-85 -0.16947573935065563 1106 86-87 -0.0545995664578065 1106 88-89 0.14113410809254745 1106 90-91 0.05133017813045626 1106 92-93 -0.1570335429185583 1106 94-95 0.04408069802808967 1106 96-97 -0.21106081825371703 1106 98-99 -0.27374903911323756 1106 100-101 0.2342699420219958 1107 1 -0.11922497750874683 1107 2 -0.09532026198917976 1107 3 -0.15921526172384404 1107 4 -0.02301694604349791 1107 5 -0.07861207456008401 1107 6 -0.0479035088741 1107 7 0.03125236909692575 1107 8 -0.05432102876126521 1107 9 -0.05104863732132259 1107 10-11 -0.029120795293138713 1107 12-13 -0.010496860341781655 1107 14-15 0.043743177970121394 1107 16-17 -0.1704074320965816 1107 18-19 -0.128109494183974 1107 20-21 -0.047914796533184756 1107 22-23 -0.07634014101706299 1107 24-25 -0.07204029076389418 1107 26-27 0.029049294804416093 1107 28-29 0.05239686093120355 1107 30-31 -0.07527257068456805 1107 32-33 0.00266998781710015 1107 34-35 -0.04244630256025772 1107 36-37 0.05559238162778968 1107 38-39 0.04632084837461292 1107 40-41 0.13531266934430164 1107 42-43 0.08010421238255105 1107 44-45 0.04185092209829833 1107 46-47 -0.1942205837953992 1107 48-49 -0.29405693521018605 1107 50-51 -0.30952677466528655 1107 52-53 -0.11457823396558808 1107 54-55 -0.45514086626337047 1107 56-57 -0.284114751934581 1107 58-59 -0.4157968736084925 1107 60-61 -0.3088983345733638 1107 62-63 -0.3650594867426804 1107 64-65 -0.3357630957667652 1107 66-67 -0.16840960060158494 1107 68-69 -0.100033009652293 1107 70-71 -0.24200423159017959 1107 72-73 -0.18857460046121233 1107 74-75 -0.23023435327840147 1107 76-77 -0.45573030605732967 1107 78-79 -0.3689387023100963 1107 80-81 -0.1903333747556779 1107 82-83 -0.10041302341224068 1107 84-85 -0.31576248742844726 1107 86-87 -0.2779193312701089 1107 88-89 -0.09123586024756847 1107 90-91 -0.01103074452897701 1107 92-93 0.05928034220129419 1107 94-95 -0.16021600029845828 1107 96-97 -0.05064471694209516 1107 98-99 -0.3003885687378407 1107 100-101 0.448779213844702 1108 1 0.04832824735790808 1108 2 0.00636769462414577 1108 3 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6 185.0 7 380.0 8 742.0 9 1319.0 10 2316.0 11 3701.0 12 5297.0 13 7450.0 14 8666.0 15 11697.0 16 15244.0 17 22049.0 18 21262.0 19 12941.0 20 11181.0 21 11772.0 22 12075.0 23 13173.0 24 14948.0 25 17452.0 26 20718.0 27 24491.0 28 29856.0 29 35647.0 30 43233.0 31 52316.0 32 63940.0 33 81228.0 34 111128.0 35 160975.0 36 240682.0 37 366031.0 38 272180.0 39 19210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77395045919239 20.076294061049296 15.343557377394578 19.806198102363734 2 15.369155135972617 23.63086001937553 37.701668137512634 23.298316707139218 3 21.479266900836226 26.044174342461908 31.690707323413964 20.785851433287906 4 12.176156440498355 17.75017690954472 39.21603715625047 30.85762949370645 5 11.721914061680296 39.76897285777905 34.32362452755841 14.185488552982239 6 28.361077335410005 38.03899121339546 16.969559900347758 16.630371550846775 7 25.08169315549504 35.812903729731175 21.67733564316383 17.428067471609953 8 25.27469068517329 32.086553359066336 20.19706615775785 22.441689798002525 9 24.700944178189587 18.55556708398278 23.02726913450138 33.716219603326245 10-11 24.876046739093688 26.399071793215768 27.012950827890037 21.71193063980051 12-13 27.761799895777838 25.09174814609289 27.221045415915214 19.925406542214056 14-15 22.803907687456118 26.378289303989437 29.275945816524985 21.541857192029457 16-17 19.738418649657323 29.599816965918645 29.201338946767102 21.46042543765693 18-19 19.452472245311462 29.102989917030296 31.480747461770637 19.96379037588761 20-21 21.818309525835787 25.97740337591209 30.746179395021816 21.458107703230304 22-23 20.476449171876805 26.543251614918923 31.728362534580423 21.25193667862385 24-25 22.075425836138045 26.58874680426168 31.189997936541058 20.14582942305921 26-27 21.182804975151058 25.42621502174792 33.62205243050981 19.768927572591203 28-29 19.574735102001323 28.93654339151044 31.675726844668173 19.812994661820063 30-31 20.479742545608854 26.455379740563757 31.28702130958529 21.777856404242097 32-33 20.649307575459062 26.946442165442775 31.9715475825471 20.432702676551063 34-35 22.040065798984337 26.420145867627927 31.426622007918265 20.113166325469475 36-37 21.25198636852776 25.80603133186666 32.9800133143528 19.96196898525278 38-39 20.358476096201084 27.40082240039829 32.13578963292797 20.104911870472666 40-41 20.508305185964993 27.89409303102346 31.103927690919015 20.493674092092533 42-43 20.005024608632826 27.908524143623364 29.88260660607797 22.20384464166584 44-45 21.022156626499 26.277586489301736 30.348067306341548 22.35218957785771 46-47 22.34063512788827 27.99858355289235 30.13249318006948 19.5282881391499 48-49 21.632921194697836 27.496300778140824 30.033484624731177 20.837293402430163 50-51 19.215426597724992 28.411538763077754 31.47713293033485 20.895901708862404 52-53 20.994820368321594 29.517255529661927 28.268862870912574 21.219061231103904 54-55 19.969852515146606 27.498417431914596 29.56965806037191 22.962071992566884 56-57 21.60482313125814 27.264675331639822 28.983670695269083 22.14683084183296 58-59 23.247593719261175 27.037341979671393 28.691318445116398 21.023745855951038 60-61 21.164141293437215 27.931877294491127 30.78995780876025 20.114023603311413 62-63 20.03854704511514 28.019848842889168 30.522229981242344 21.41937413075335 64-65 19.679498613282412 29.68989170895602 29.391563814073756 21.239045863687817 66-67 20.88316566547822 27.694239350830014 28.21223815529122 23.210356828400545 68-69 19.989659259786034 27.91271226132578 29.29107930455899 22.8065491743292 70-71 21.831191571761156 28.00760798477004 28.36238885592277 21.798811587546034 72-73 21.13795097361454 26.719199377931247 30.44965917953608 21.69319046891813 74-75 19.751003458916767 30.080422437842667 28.413159272216866 21.755414831023696 76-77 20.310290882382038 27.763931238289167 28.44966323289996 23.476114646428833 78-79 20.59968581779181 27.990661968506004 29.182718229854306 22.22693398384789 80-81 21.529949882429474 27.69957553367227 28.624809538076647 22.145665045821612 82-83 20.6066685863441 27.31608693574016 29.948570908959493 22.128673568956252 84-85 20.155796978956218 28.12494535331197 29.256292092522234 22.46296557520958 86-87 20.028458470335327 28.43938126907265 28.647142696607897 22.88501756398413 88-89 19.734358126789502 28.246572190592612 27.808324280620344 24.21074540199754 90-91 20.43329339769434 26.798061449806987 28.52194174348453 24.24670340901415 92-93 21.360122548476713 27.926497727280676 28.466989903040744 22.246389821201866 94-95 20.56469992993566 27.25345454503062 28.862923373393972 23.31892215163975 96-97 18.634640101891225 27.600484974250318 30.3460979100058 23.418777013852658 98-99 19.91590530472159 28.190200785047033 27.839033881529478 24.054860028701896 100-101 19.41855239358741 26.67235187515702 29.122276477168136 24.786819254087433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5493.0 1 3690.0 2 1986.0 3 2060.0 4 1762.0 5 1794.0 6 2898.0 7 5102.5 8 7502.5 9 8353.5 10 6844.5 11 5147.0 12 4757.0 13 4803.5 14 4927.5 15 4874.5 16 4953.0 17 5170.5 18 5288.5 19 5486.5 20 5851.5 21 6508.5 22 7490.5 23 8675.5 24 9701.0 25 10957.0 26 12951.0 27 15065.0 28 17145.5 29 19499.0 30 22190.5 31 25235.5 32 27632.5 33 29667.5 34 32107.5 35 33992.0 36 36622.5 37 39897.0 38 40742.5 39 40876.0 40 42091.5 41 43999.5 42 45602.0 43 46789.0 44 48539.5 45 50125.5 46 53823.0 47 79910.5 48 127001.0 49 123890.0 50 79720.5 51 55924.0 52 46313.0 53 43443.5 54 41542.0 55 37957.0 56 34137.5 57 30244.0 58 27987.0 59 25701.0 60 22453.0 61 18807.0 62 15413.5 63 11926.5 64 8514.5 65 6186.5 66 4291.5 67 3307.5 68 2332.0 69 1315.5 70 938.5 71 687.5 72 494.5 73 392.5 74 286.0 75 217.5 76 155.5 77 80.0 78 29.5 79 10.0 80 9.5 81 8.5 82 4.5 83 3.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4030156811221486 2 0.0 3 0.0 4 0.0 5 5.828980056727634E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014281001138982702 16-17 0.002244157321840139 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002215012421556501 36-37 0.007227935270342266 38-39 0.01291119082565171 40-41 0.002419026723541968 42-43 5.537531053891252E-4 44-45 4.080286039709344E-4 46-47 3.49738803403658E-4 48-49 1.4572450141819084E-4 50-51 5.537531053891252E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.040143019854672E-4 60-61 0.002564751224960159 62-63 0.0 64-65 2.3315920226910534E-4 66-67 0.0012532307121964413 68-69 0.0012532307121964413 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1248276079148223 78-79 5.8289800567276334E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14826010774286738 88-89 0.43801870636279805 90-91 0.33408799195134437 92-93 0.0 94-95 0.0 96-97 6.411878062400398E-4 98-99 0.0 100-101 1.74869401701829E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1715566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.19607918829719 #Duplication Level Percentage of deduplicated Percentage of total 1 67.69053151957058 25.178223706998743 2 14.996023364295072 11.155865451357487 3 6.311614599648867 7.043019493636557 4 3.234274533834172 4.812093267087554 5 1.9667907027973248 3.6578451364027984 6 1.2730068371033099 2.8410517872083076 7 0.8259868900017637 2.1506431638300643 8 0.610572374955919 1.8168718727237636 9 0.4649041363618054 1.5563349963972566 >10 2.3879023451929817 15.835049014444744 >50 0.15849370587410322 4.046669469861049 >100 0.07032376281118594 4.505467263162787 >500 0.0031394188698416524 0.7531343830902826 >1k 0.004081244530794149 4.141567656077449 >5k 0.0014127384914287438 4.2795362231944605 >10k+ 9.418256609524957E-4 6.226627114526715 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35254 2.0549486291987598 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18085 1.0541710432591924 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15312 0.8925334262861353 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13589 0.7921000999087182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13243 0.7719318289124405 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11161 0.6505724641313713 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9273 0.5405213206603535 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9081 0.5293296789514365 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9046 0.5272895359315817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8906 0.519128963852163 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8256 0.48124059348343345 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8150 0.4750618746233021 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7871 0.45879902026503205 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6806 0.3967203826608827 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5907 0.3443178519509013 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4807 0.28019907132689736 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4766 0.27780918950363903 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4694 0.2736123238627951 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4685 0.27308771565768963 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4678 0.2726796870537187 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4463 0.2601473799317543 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4268 0.2487808688211354 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3335 0.1943964848918666 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3298 0.19223976227087736 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3126 0.18221391657330582 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2916 0.16997305845417782 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2910 0.16962331965077415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 0.16082155976511542 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2618 0.15260269788512945 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2265 0.1320263982848809 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1772 0.10328952660521368 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.828980056727634E-5 0.0 8 0.0 0.0 0.0 5.828980056727634E-5 0.0 9 0.0 0.0 0.0 5.828980056727634E-5 0.0 10-11 0.0 0.0 0.0 8.743470085091451E-5 0.0 12-13 0.0 0.0 0.0 2.040143019854672E-4 0.0 14-15 0.0 0.0 0.0 3.2059390312001983E-4 0.0 16-17 0.0 0.0 0.0 4.080286039709344E-4 0.0 18-19 0.0 0.0 0.0 6.411878062400397E-4 0.0 20-21 0.0 0.0 0.0 9.034919087927832E-4 0.0 22-23 0.0 0.0 0.0 0.0016904042164510137 0.0 24-25 0.0 0.0 0.0 0.002856200227796541 0.0 26-27 0.0 0.0 0.0 0.004400879942829364 0.0 28-29 0.0 0.0 0.0 0.01291119082565171 0.0 30-31 0.0 0.0 0.0 0.03820896427184964 0.0 32-33 0.0 0.0 0.0 0.08128512689106684 0.0 34-35 0.0 0.0 0.0 0.14178993987989968 0.0 36-37 0.0 0.0 0.0 0.22540665879365762 0.0 38-39 0.0 0.0 0.0 0.35836569388761497 0.0 40-41 0.0 0.0 0.0 0.5104437835676389 0.0 42-43 0.0 0.0 0.0 0.6599862669229863 0.0 44-45 0.0 0.0 0.0 0.8404223445789902 0.0 46-47 0.0 0.0 0.0 1.0553951290711052 0.0 48-49 0.0 0.0 0.0 1.2641600498028056 0.0 50-51 0.0 0.0 0.0 1.4962408907614164 0.0 52-53 0.0 0.0 0.0 1.7280302827171907 0.0 54-55 0.0 0.0 0.0 1.9823778274925008 0.0 56-57 0.0 0.0 0.0 2.3097042025780414 0.0 58-59 0.0 0.0 0.0 2.632717132421603 0.0 60-61 0.0 0.0 0.0 2.999359977989771 0.0 62-63 0.0 0.0 0.0 3.3623888559227684 0.0 64-65 0.0 0.0 0.0 3.7292065708926385 0.0 66-67 0.0 0.0 0.0 4.160580239990766 0.0 68-69 0.0 0.0 0.0 4.6656030721056485 0.0 70-71 0.0 0.0 0.0 5.203209902737639 0.0 72-73 0.0 0.0 0.0 5.728546730350216 0.0 74-75 0.0 0.0 0.0 6.297630053288536 0.0 76-77 0.0 0.0 0.0 6.86315769839225 0.0 78-79 0.0 0.0 0.0 7.5844065457114445 0.0 80-81 0.0 0.0 0.0 8.356396664424452 0.0 82-83 0.0 0.0 0.0 9.068319143652882 0.0 84-85 0.0 0.0 0.0 9.813758258207496 0.0 86-87 0.0 0.0 0.0 10.562782195496997 0.0 88-89 0.0 0.0 0.0 11.399269978537696 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 775 0.0 30.003832 3 CTTTGCG 860 0.0 27.03834 2 TTGCGTT 885 0.0 26.274546 4 GTAAGCG 335 0.0 24.082016 94-95 GTACATG 9335 0.0 23.530544 1 TACATGG 9345 0.0 23.207014 2 GAGTACT 10565 0.0 22.998959 12-13 ACATGGG 9350 0.0 21.824245 3 AGTACTT 10875 0.0 21.143272 12-13 GCTTTGC 1155 0.0 21.03941 1 GTACTTT 11580 0.0 20.983072 14-15 GTACACG 185 6.381013E-6 20.604574 1 CATGGGA 6545 0.0 20.15661 4 AGAGTAC 16645 0.0 19.616083 10-11 CATATAG 345 4.9112714E-11 19.335451 1 ATGGGAT 2495 0.0 19.210258 5 CATGGGG 2595 0.0 19.201464 4 ATGGGAG 2090 0.0 18.845781 5 ACTTTTT 13260 0.0 18.43088 16-17 TACTTTT 12755 0.0 17.859467 14-15 >>END_MODULE