FastQCFastQC Report
Fri 27 May 2016
SRR939021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709740
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT269660.7268973027759358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156300.4213233272412622No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT147370.39725155940847606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124300.33506391283486175No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT111100.29948190439222155No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC99000.26686506331980137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77960.2101494983475931No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77110.20785823265242306No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63190.1703353873856389No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62430.16828672629348687No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC61130.16478243758322686No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA56180.15143918441723678No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55870.15060354634017478No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54610.14720708189792275No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC53390.14391844172367874No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA45240.12194924711704863No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA40680.10965728056413658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT129250.022.96739412-13
GTACATG153500.022.2651161
TACATGG150550.021.864962
TTTGCGT9700.020.9962333
ACATGGG153500.020.7659263
GTACTTT141300.020.72477514-15
AGTACTT136950.019.96478712-13
TATACCG2451.8127139E-719.3320775
CATGGGA77750.018.0316684
CATGGGG75300.017.486164
ACTTTTT169900.017.29333516-17
CTTTGCG11850.017.1867892
GTATCGC2253.6437334E-516.8326666
GTAAGCG5550.016.28321894-95
TACTTTT164700.016.27103414-15
GTATAGG11400.016.2390481
AGAGTAC272450.016.17705710-11
GGACCGA8850.016.0480926
GTGTAGC22600.015.9626861
AGTACAT117200.015.8417472
Produced by FastQC (version 0.11.5)