FastQCFastQC Report
Fri 27 May 2016
SRR939021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709740
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT282570.761697585275518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153840.41469213475877015No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT137420.37043027274148593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129840.34999757395396985No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT106170.2861925633602355No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC93530.2521200946697073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84080.22664661135281716No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77240.20820866152344908No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC69020.18605077444780496No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67540.18206127653150894No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT64140.1728962137508289No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA61080.16464765724821684No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG60870.16408157984117486No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53460.14410713419269275No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC53040.14297497937860873No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46910.12645091030638267No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA46060.12415964461121264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT9150.024.3745143
TACATGG147600.022.472432
GTACATG150100.022.4402711
GAGTACT144250.020.85846712-13
ACATGGG152800.020.8381123
CTTTGCG10850.020.5554642
GTAAGCG6350.019.80408594-95
AGTACTT151700.019.75589612-13
GTACTTT151250.019.61085514-15
CATGGGG71950.017.8070584
TTGCGTT12850.016.986894
TACTTTT176100.016.7087614-15
AGAGTAC279000.016.56753310-11
ACTTTTT182850.016.33752616-17
GTATAGG10000.016.198261
CATGGGA82250.016.1540474
AGTACAT118300.016.0849172
TATAGGG8350.015.9122192
GTGTACG3755.642505E-815.24541951
TCTATAC8300.014.8646423
Produced by FastQC (version 0.11.5)