FastQCFastQC Report
Fri 27 May 2016
SRR939020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723494
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT269160.7228694339241584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153870.41324089685655463No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT147240.39543504031428545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124770.33508849483845016No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT110680.29724769262418577No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC97290.26128684509764216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80180.2153353812306398No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT75970.20402879660877657No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63420.17032389470749784No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62940.1690347829216322No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC61520.16522116055511302No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55800.14985924510688078No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA55710.14961753664703098No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55450.14891926776302045No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC54030.14510564539650123No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44790.12029024351858765No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA42930.11529493534835827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG151500.024.0348661
TACATGG151350.023.1397882
TTTGCGT10700.023.0000953
ACATGGG149950.022.188043
GAGTACT134250.021.27402712-13
GTACTTT140100.020.1162914-15
AGTACTT139400.019.80910312-13
CTTTGCG12500.019.688082
CATGGGG76200.018.8190524
CATGGGA75200.018.251154
GTATAGG10050.017.4553591
ACTTTTT170100.016.91736416-17
AGTACAT112200.016.9145952
TACTTTT166300.016.39209614-15
AGAGTAC276900.015.9966110-11
TTGCGTT14500.015.9933024
TATGACG2205.8651477E-415.0586284
ACCGTGC5409.094947E-1214.8980788
TTAGCGC2601.25621E-414.562194
ATGGGAT33850.014.540295
Produced by FastQC (version 0.11.5)