##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939020_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3723494 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.890839893927584 33.0 31.0 34.0 30.0 34.0 2 31.954905795470598 33.0 31.0 34.0 30.0 34.0 3 32.00768391193863 34.0 31.0 34.0 30.0 34.0 4 35.535672408764455 37.0 35.0 37.0 33.0 37.0 5 35.57809976328685 37.0 35.0 37.0 33.0 37.0 6 35.66301275092695 37.0 35.0 37.0 33.0 37.0 7 35.58386880709355 37.0 35.0 37.0 33.0 37.0 8 35.56940309290145 37.0 35.0 37.0 33.0 37.0 9 37.18417593797653 39.0 37.0 39.0 34.0 39.0 10-11 37.076009656521535 39.0 37.0 39.0 33.0 39.0 12-13 36.97099055349626 39.0 37.0 39.0 33.0 39.0 14-15 38.17649833731436 40.0 38.0 41.0 33.0 41.0 16-17 38.11717153297414 40.0 38.0 41.0 33.0 41.0 18-19 38.08093768917044 40.0 38.0 41.0 33.0 41.0 20-21 37.95990311787799 40.0 38.0 41.0 33.0 41.0 22-23 38.03924861971041 40.0 38.0 41.0 33.0 41.0 24-25 37.97704884175992 40.0 38.0 41.0 33.0 41.0 26-27 37.88506265888974 40.0 38.0 41.0 33.0 41.0 28-29 37.76767694536368 40.0 38.0 41.0 32.0 41.0 30-31 37.594530030127615 40.0 37.0 41.0 32.0 41.0 32-33 37.39638831162344 40.0 37.0 41.0 31.5 41.0 34-35 37.06780821991387 40.0 36.0 41.0 31.0 41.0 36-37 36.80278979904359 40.0 36.0 41.0 30.0 41.0 38-39 36.60207818248129 39.0 36.0 41.0 30.0 41.0 40-41 36.47186594096835 39.0 35.5 41.0 30.0 41.0 42-43 36.26443227785515 39.0 35.0 41.0 30.0 41.0 44-45 36.18390186743956 39.0 35.0 41.0 29.5 41.0 46-47 35.94679043393114 39.0 35.0 41.0 28.0 41.0 48-49 35.74476526617204 39.0 35.0 41.0 27.0 41.0 50-51 34.568628551570114 38.0 34.0 40.0 25.5 40.5 52-53 34.72313974455176 38.0 34.5 39.5 26.0 40.5 54-55 35.23475625313214 39.0 35.0 40.5 26.0 41.0 56-57 35.117928617583374 39.0 35.0 41.0 26.0 41.0 58-59 34.92300699289431 38.5 35.0 40.5 26.0 41.0 60-61 34.637962220430595 38.0 34.0 40.0 25.5 41.0 62-63 34.28921706332815 38.0 34.0 40.0 24.5 41.0 64-65 33.889491966416486 37.0 34.0 40.0 23.0 41.0 66-67 33.48985858443709 36.5 33.0 39.0 22.0 41.0 68-69 33.07861782508579 36.0 33.0 39.0 22.0 41.0 70-71 32.64067835210692 35.5 32.5 39.0 20.5 40.0 72-73 32.20335886132756 35.0 32.0 38.0 19.5 40.0 74-75 31.74263554607581 35.0 31.5 37.0 18.0 39.0 76-77 31.340722987602504 35.0 31.0 36.5 16.0 39.0 78-79 30.90779923373047 35.0 31.0 36.0 11.5 39.0 80-81 30.450888332302938 34.5 31.0 36.0 7.5 37.5 82-83 30.081374510070383 34.0 30.0 35.0 4.5 37.0 84-85 29.72472709772058 34.0 30.0 35.0 2.0 36.5 86-87 29.363549665985765 34.0 30.0 35.0 2.0 36.0 88-89 28.942569129962344 34.0 29.0 35.0 2.0 36.0 90-91 28.50673480338628 34.0 29.0 35.0 2.0 35.5 92-93 28.09408891218839 34.0 29.0 35.0 2.0 35.0 94-95 27.622792866055377 34.0 27.0 35.0 2.0 35.0 96-97 27.043864982728586 33.5 26.0 35.0 2.0 35.0 98-99 26.35589650473453 33.0 25.0 35.0 2.0 35.0 100-101 23.72884097570723 31.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30870669374685633 1101 2 0.4057029565313819 1101 3 0.3808342922959511 1101 4 0.23105322629528047 1101 5 0.10127420214273286 1101 6 0.20813698565675054 1101 7 0.23096036076920257 1101 8 0.23015727339854664 1101 9 0.2866542268288015 1101 10-11 0.34027581296813025 1101 12-13 0.5213370204580272 1101 14-15 0.3016898777843906 1101 16-17 0.5437705125943495 1101 18-19 0.5215936076967438 1101 20-21 0.529283523870582 1101 22-23 0.41510925460799086 1101 24-25 0.49582763426243304 1101 26-27 0.4241037676434658 1101 28-29 0.39915052555369357 1101 30-31 0.5163592437427482 1101 32-33 0.5091491209933778 1101 34-35 0.3588221220111478 1101 36-37 0.28434989649356623 1101 38-39 0.3525709212387156 1101 40-41 0.3829811489202086 1101 42-43 0.44498878074873005 1101 44-45 0.6273086760349926 1101 46-47 0.6161939170783839 1101 48-49 0.6578292531437668 1101 50-51 0.621769551638593 1101 52-53 0.517837017982167 1101 54-55 0.4808808880158537 1101 56-57 0.5708605375029592 1101 58-59 0.4833933546698219 1101 60-61 0.48255439187729365 1101 62-63 0.421017186649415 1101 64-65 0.45786483221463925 1101 66-67 0.39412609385649233 1101 68-69 0.3740298977926031 1101 70-71 0.4158753758619227 1101 72-73 0.3838901602723368 1101 74-75 0.2886378396023268 1101 76-77 0.2827165480044407 1101 78-79 0.3356319234896219 1101 80-81 0.14843055217578538 1101 82-83 0.10190530341305504 1101 84-85 -0.05137944214153478 1101 86-87 -0.05193766205065842 1101 88-89 -0.22357138840324708 1101 90-91 -0.27507187102217756 1101 92-93 -0.236296335647026 1101 94-95 -0.06485790809593439 1101 96-97 -0.12154181851914458 1101 98-99 -0.3590499557747542 1101 100-101 -0.3119238187550053 1102 1 0.30271502878819234 1102 2 0.18200616073989195 1102 3 0.2293824920636638 1102 4 0.18418569879647606 1102 5 0.2713056293477649 1102 6 0.2794904050733038 1102 7 0.33278792106161603 1102 8 0.16564951907730574 1102 9 0.3493651648625544 1102 10-11 0.26211942846311587 1102 12-13 0.3227068366771917 1102 14-15 0.47364792929767674 1102 16-17 0.3339341390205348 1102 18-19 0.4344463097531701 1102 20-21 0.5054142200514917 1102 22-23 0.4960701755617336 1102 24-25 0.5379554610028876 1102 26-27 0.5740183676298045 1102 28-29 0.5271958900409501 1102 30-31 0.5186155121037572 1102 32-33 0.5244476794411455 1102 34-35 0.5236373163815742 1102 36-37 0.4335744643694923 1102 38-39 0.3642058191002988 1102 40-41 0.3756435797461961 1102 42-43 0.26749680835000333 1102 44-45 0.4375805896784968 1102 46-47 0.36556708097808865 1102 48-49 0.34808169558415614 1102 50-51 0.3848767457357454 1102 52-53 0.28234989630421836 1102 54-55 0.32301725475767995 1102 56-57 0.3039649987996711 1102 58-59 0.3269490696387294 1102 60-61 0.2325663478792066 1102 62-63 0.09088806182874976 1102 64-65 0.13503740856225477 1102 66-67 0.26540606926128874 1102 68-69 0.23086360688599683 1102 70-71 0.22040157658611292 1102 72-73 0.20035699090703218 1102 74-75 0.08883084363320748 1102 76-77 0.03495573636271132 1102 78-79 0.09797905746516378 1102 80-81 -0.05697697668942325 1102 82-83 -0.078821621503252 1102 84-85 -0.12549469799997937 1102 86-87 -0.003971890376131881 1102 88-89 0.017598250183894493 1102 90-91 -0.030569923901868634 1102 92-93 -0.11630077644773706 1102 94-95 -0.2331967070301424 1102 96-97 -0.19299430195154343 1102 98-99 -0.3098185558977278 1102 100-101 -0.08063154574823983 1103 1 0.07056493802143748 1103 2 0.18238306829373485 1103 3 0.24044108326972946 1103 4 0.23686542912015085 1103 5 0.23533876261114983 1103 6 0.13361474172630494 1103 7 0.24116247737139673 1103 8 0.2111620742180591 1103 9 0.2578127959264904 1103 10-11 0.2853006023633142 1103 12-13 0.3033666596912994 1103 14-15 0.27636953137309916 1103 16-17 0.3189999495509568 1103 18-19 0.27613570415130084 1103 20-21 0.3450598280898802 1103 22-23 0.383560922790231 1103 24-25 0.43189552899453787 1103 26-27 0.34719812040317066 1103 28-29 0.24960734868965773 1103 30-31 0.23518280287165538 1103 32-33 0.31210006440092997 1103 34-35 0.22038215326298882 1103 36-37 0.11374673789645584 1103 38-39 0.32630646701056065 1103 40-41 0.37572089728484315 1103 42-43 0.11378900603411068 1103 44-45 0.15357638149536257 1103 46-47 0.14225116777640068 1103 48-49 0.07698823939955446 1103 50-51 -0.07435577986205288 1103 52-53 0.0345962447291015 1103 54-55 0.16386698974842062 1103 56-57 0.2680510226968522 1103 58-59 0.21197661272461943 1103 60-61 0.08220828570304661 1103 62-63 0.08337371977545871 1103 64-65 -0.08658513932456913 1103 66-67 -0.03245445517158174 1103 68-69 -0.13027285795181598 1103 70-71 -0.12850269342633425 1103 72-73 -0.021913014130767294 1103 74-75 0.019404925327815903 1103 76-77 -0.03788533510925873 1103 78-79 -0.044707350095066545 1103 80-81 0.05680457593078714 1103 82-83 -0.027430214748328297 1103 84-85 -0.11122324794393634 1103 86-87 -0.00949970306420056 1103 88-89 -0.018449147073784644 1103 90-91 -0.1706869341310977 1103 92-93 -0.07323816159906116 1103 94-95 -0.1314690287106437 1103 96-97 -0.1488484177202416 1103 98-99 -0.2127274995638473 1103 100-101 -0.12889832709366544 1104 1 0.12371302361241021 1104 2 0.07603464347115008 1104 3 0.16167473039476477 1104 4 0.15277427489283468 1104 5 0.14670971905638908 1104 6 0.11603388575933593 1104 7 0.11567270170797173 1104 8 0.11160138913766815 1104 9 0.1953918561345418 1104 10-11 0.20141866713378676 1104 12-13 0.18377316357683782 1104 14-15 0.21049457687015405 1104 16-17 0.14216688325090132 1104 18-19 0.22486098108537078 1104 20-21 0.16938075768459981 1104 22-23 0.17374219385880707 1104 24-25 0.28896305468162353 1104 26-27 0.18344416488843507 1104 28-29 0.22982521611473317 1104 30-31 0.3283704654845394 1104 32-33 0.30225675417941034 1104 34-35 0.31645717706129517 1104 36-37 0.30340631483168323 1104 38-39 0.3469870105759938 1104 40-41 0.2997390337957171 1104 42-43 0.38547903024169017 1104 44-45 0.40409341859454884 1104 46-47 0.47280753493975425 1104 48-49 0.4633880477865233 1104 50-51 0.4199349988096017 1104 52-53 0.4701988252902751 1104 54-55 0.4569292027367098 1104 56-57 0.5305810409414633 1104 58-59 0.45435945887657425 1104 60-61 0.3775571474445769 1104 62-63 0.4513955792969071 1104 64-65 0.5253385608742889 1104 66-67 0.46629660922035754 1104 68-69 0.48232985336611733 1104 70-71 0.5062279627706303 1104 72-73 0.41359070156102007 1104 74-75 0.38462581798518514 1104 76-77 0.35314738409251234 1104 78-79 0.26707556783817665 1104 80-81 0.15537992416777513 1104 82-83 0.10817595569519511 1104 84-85 0.08550017893322703 1104 86-87 0.22054928043076316 1104 88-89 0.23525421726746387 1104 90-91 0.31701142220160605 1104 92-93 0.36309764629987384 1104 94-95 0.22689228567039876 1104 96-97 0.12019809562491801 1104 98-99 -0.019213582881992153 1104 100-101 0.5208983866184482 1105 1 0.084885893819024 1105 2 0.08664800800685768 1105 3 0.12452135746063675 1105 4 0.0976364496864619 1105 5 0.11856105531639827 1105 6 0.20018281180156094 1105 7 0.15744401603571134 1105 8 0.2022060313341143 1105 9 0.18282260182739662 1105 10-11 0.1123547345201743 1105 12-13 0.22262644458665903 1105 14-15 0.22087111883396915 1105 16-17 0.17150609889612412 1105 18-19 0.24103448137039152 1105 20-21 0.16029933298845123 1105 22-23 0.22358297561427065 1105 24-25 0.14084161664914063 1105 26-27 0.13349000852390702 1105 28-29 0.23938822612765165 1105 30-31 0.20814882009898383 1105 32-33 0.10403286421360747 1105 34-35 0.290839532337813 1105 36-37 0.29074458318661556 1105 38-39 0.2216833779666061 1105 40-41 0.1459188032620773 1105 42-43 0.11780375092338602 1105 44-45 4.922382881744625E-5 1105 46-47 0.219259047937733 1105 48-49 0.2902760016750534 1105 50-51 0.23464462352384174 1105 52-53 0.14533411438170418 1105 54-55 0.12793331068987612 1105 56-57 0.18698933332628087 1105 58-59 0.20987390900379665 1105 60-61 0.42558377744160225 1105 62-63 0.29001349935872867 1105 64-65 0.19888763252586728 1105 66-67 0.015306872585227893 1105 68-69 0.06381739934307262 1105 70-71 -0.030218576755828508 1105 72-73 0.20853609015743046 1105 74-75 0.09385952808828435 1105 76-77 0.16583704460469306 1105 78-79 0.16652425911200908 1105 80-81 0.13107604229226766 1105 82-83 0.09100467984371363 1105 84-85 0.15732149264867346 1105 86-87 0.007734710331874339 1105 88-89 0.03304487401457834 1105 90-91 0.16822419474014438 1105 92-93 0.30887945903741354 1105 94-95 0.2242096133005056 1105 96-97 0.09601904432942732 1105 98-99 0.15035094405948968 1105 100-101 0.4071054766003641 1106 1 0.13501419262455983 1106 2 0.21929428295358733 1106 3 0.15819116116824006 1106 4 0.1585288751920686 1106 5 0.20952977330131262 1106 6 0.1910090179534194 1106 7 0.18721887786410463 1106 8 0.21323763004216545 1106 9 0.21945253083856642 1106 10-11 0.20173369198983693 1106 12-13 0.238563159412287 1106 14-15 0.26196966185556647 1106 16-17 0.3441634771744191 1106 18-19 0.2864925128756113 1106 20-21 0.3248299119833078 1106 22-23 0.3272199844301653 1106 24-25 0.40081811268952094 1106 26-27 0.33141013703257016 1106 28-29 0.33945633419244103 1106 30-31 0.25113531104557296 1106 32-33 0.37217965712503087 1106 34-35 0.27696055865194324 1106 36-37 0.2115656192304769 1106 38-39 0.24639515875403362 1106 40-41 0.44257692939363125 1106 42-43 0.43869919073551245 1106 44-45 0.3835226206660849 1106 46-47 0.3825407882991101 1106 48-49 0.3604305516562647 1106 50-51 0.2609473094998762 1106 52-53 0.1618403042028831 1106 54-55 0.09455177388958447 1106 56-57 0.1319232952124736 1106 58-59 0.22259695337705665 1106 60-61 0.25506560289036173 1106 62-63 0.12415738861539438 1106 64-65 0.02908144480324637 1106 66-67 0.19583674188260858 1106 68-69 0.30400611782401654 1106 70-71 0.2875316822265148 1106 72-73 0.2200219334096758 1106 74-75 0.22274707728539056 1106 76-77 0.17399532240546733 1106 78-79 0.20671545771855904 1106 80-81 0.21301309670431223 1106 82-83 0.25206689856408104 1106 84-85 0.33652468552422476 1106 86-87 0.3591650750820392 1106 88-89 0.3902203543481342 1106 90-91 0.43134644394698185 1106 92-93 0.5549422596334068 1106 94-95 0.6463596151129032 1106 96-97 0.6239221744387251 1106 98-99 0.6664919830794958 1106 100-101 1.2503147972939566 1107 1 0.02415289361002948 1107 2 0.011375080375906066 1107 3 0.038517139574523185 1107 4 0.14325725792463828 1107 5 0.03719309225139966 1107 6 0.006615658114711209 1107 7 0.08918303908209424 1107 8 0.04389090584250255 1107 9 0.0464458040009319 1107 10-11 0.0979954922197166 1107 12-13 0.0907483452953457 1107 14-15 0.04342996974499869 1107 16-17 0.12661962083103617 1107 18-19 0.16770843117374312 1107 20-21 0.18231384859652877 1107 22-23 0.058194151112537895 1107 24-25 0.16310787546014893 1107 26-27 0.1817528854262278 1107 28-29 0.11051736564088088 1107 30-31 0.11448685897193656 1107 32-33 0.15247680622717752 1107 34-35 0.005517586030364896 1107 36-37 0.02602587059185879 1107 38-39 0.09154392173192605 1107 40-41 0.07277590812024215 1107 42-43 -0.022694225631241238 1107 44-45 0.23351188033709036 1107 46-47 0.2821477299433255 1107 48-49 0.3081575588730061 1107 50-51 0.4140734989442123 1107 52-53 0.239169730820052 1107 54-55 0.3077243163327239 1107 56-57 0.3996612311850427 1107 58-59 0.2880581280298813 1107 60-61 0.38222751806060984 1107 62-63 0.26519693940583977 1107 64-65 0.30385523840050155 1107 66-67 0.2834819718575474 1107 68-69 0.3864554781149465 1107 70-71 0.4412973923130039 1107 72-73 0.32357076422123043 1107 74-75 0.41557442178615034 1107 76-77 0.4454899239769503 1107 78-79 0.37841261812631544 1107 80-81 0.2987505050817738 1107 82-83 0.38318050109653967 1107 84-85 0.30954215269084884 1107 86-87 0.3528508921949616 1107 88-89 0.41030495799231304 1107 90-91 0.3551211985210436 1107 92-93 0.2705664234893703 1107 94-95 0.31120218450108084 1107 96-97 0.15297570649955006 1107 98-99 0.25610683865033224 1107 100-101 0.40610515360908295 1108 1 0.10884851784994964 1108 2 0.09457754628229864 1108 3 0.054318826854149904 1108 4 0.12110647356141868 1108 5 0.10330477700698992 1108 6 0.16897096585585558 1108 7 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-1.771611433324395 2316 68-69 -1.952686759427543 2316 70-71 -1.8533922228946835 2316 72-73 -1.8516233046841322 2316 74-75 -2.0450732910265152 2316 76-77 -1.990970715611077 2316 78-79 -1.749337771684246 2316 80-81 -1.6819294081741774 2316 82-83 -1.8243398258066605 2316 84-85 -2.0158582021711773 2316 86-87 -2.0637230762259016 2316 88-89 -2.1227804896774884 2316 90-91 -2.4028628984028906 2316 92-93 -2.6626138332777955 2316 94-95 -2.812794553635836 2316 96-97 -2.926300709513452 2316 98-99 -3.221847805920099 2316 100-101 -3.9494570398444893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10432.0 3 2475.0 4 2410.0 5 2782.0 6 3353.0 7 4184.0 8 5298.0 9 6566.0 10 8233.0 11 10768.0 12 14568.0 13 21990.0 14 21356.0 15 19918.0 16 24953.0 17 33907.0 18 29444.0 19 20856.0 20 20746.0 21 22235.0 22 24305.0 23 27316.0 24 30454.0 25 36171.0 26 41617.0 27 49222.0 28 58471.0 29 70099.0 30 84345.0 31 102583.0 32 128685.0 33 165023.0 34 224274.0 35 321921.0 36 493619.0 37 760170.0 38 702365.0 39 116330.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89944743167232 19.20745929535418 14.36031247205646 22.53278080091703 2 14.998711095305564 21.144326951171884 40.125525070741915 23.73143688278064 3 19.972489668841096 25.071658848238076 30.36380894163718 24.592042541283643 4 12.558717253839205 16.613084484019446 38.447138340431025 32.381059921710325 5 13.035826389010246 38.12669295180893 34.668077263800036 14.169403395380787 6 30.524670792240883 36.60535468102509 17.73272074209803 15.137253784635995 7 27.181676857928338 33.551325168675305 21.57436377306878 17.69263420032758 8 25.37883705833256 34.28340638175765 20.352057116206804 19.985699443702984 9 25.849918251501503 17.570201287868155 21.671685556725823 34.90819490390452 10-11 24.732393851308235 26.324483106580658 27.567062146558552 21.37606089555256 12-13 26.435712218716723 24.14494896743343 27.67199373445324 21.747345079396613 14-15 23.041612232561583 25.466491151262673 27.209475788646515 24.282420827529233 16-17 21.33818208014679 28.54549826330297 27.803684085247948 22.312635571302287 18-19 21.251297307447718 28.13389220999894 29.494490652329404 21.12031983022394 20-21 22.52711077153289 26.455252339805362 29.158936318419464 21.858700570242284 22-23 21.85311213884924 26.467124860234293 29.311616662909106 22.368146338007357 24-25 22.409843575270266 26.61304472987056 29.254781825694238 21.72232986916493 26-27 22.027287460356327 26.151896906210126 30.240874610017304 21.57994102341624 28-29 21.347056075237557 27.480839363922676 29.611785782710555 21.56031877812921 30-31 21.80707722732079 26.73233970099612 29.2657862312484 22.19479684043469 32-33 21.77927970013294 26.772179729344785 29.430000660055978 22.0185399104663 34-35 22.517542375680144 26.594099992383867 29.25491998681668 21.633437645119308 36-37 22.254619269483356 26.26091053412669 29.86115210269609 21.623318093693864 38-39 21.877301786706433 26.99241036688944 29.59355933238023 21.5367285140239 40-41 21.972525230925164 27.052649399018115 29.349724018404356 21.625101351652365 42-43 21.50425393889733 27.165630571968563 28.768683058730876 22.561432430403226 44-45 21.888405756581548 26.786019390934534 28.779706660215897 22.54586819226802 46-47 22.53734183377943 27.22589417352465 28.39656695924483 21.84019703345109 48-49 22.22505442420568 27.108617152391556 28.53049511573485 22.13583330766791 50-51 21.60843692780813 27.508945710894288 28.994935941524254 21.88768141977333 52-53 22.05934376367204 27.904719748751205 27.974526088836406 22.061410398740353 54-55 21.58953776412644 27.281726000418416 28.42918840525294 22.6995478302022 56-57 22.238791037015325 27.337149171587015 27.99410327823542 22.42995651316224 58-59 22.722301671826088 27.05342271773812 28.1069010622004 22.11737454823539 60-61 22.090785038939952 27.302714677134137 28.934532947280466 21.67196733664544 62-63 21.844409179492978 27.2358683719706 28.828139000447944 22.091583448088475 64-65 21.618658720722646 27.844356475395454 28.444584700185118 22.092400103696786 66-67 21.881836123143746 27.228365880558524 27.905643243932666 22.98415475236506 68-69 21.57273615936649 27.37972560244443 28.33292236977128 22.714615868417802 70-71 22.357659428897538 27.15876579265083 27.915540325099737 22.568034453351896 72-73 21.944649939018095 26.749318715689235 28.675814582591435 22.63021676270123 74-75 21.42277213544833 28.104542052691162 27.999981087163857 22.47270472469665 76-77 21.7674427022918 27.041598074021167 28.03577497037932 23.15518425330771 78-79 21.838331697347975 27.04027899930901 28.361085667701463 22.760303635641552 80-81 22.142439993977625 27.178280557650407 27.95716551339067 22.722113934981294 82-83 21.878671268654045 26.88036818059843 28.617376675422 22.623583875325522 84-85 21.712258598671916 27.072159894001878 28.2940556368565 22.921525870469704 86-87 21.537147607808905 27.274873336794208 28.264666813240712 22.92331224215617 88-89 21.648633747370567 27.039248849423842 27.915096839653568 23.397020563552026 90-91 21.71016948992269 26.525246524103686 28.335186054996992 23.429397930976627 92-93 22.047649386630873 26.942875963852263 28.217830476564114 22.79164417295275 94-95 21.76827306100977 26.7230522690373 28.291466532723163 23.217208137229765 96-97 21.05948711450473 26.58413299591092 28.874959759634482 23.481420129949868 98-99 21.53522064264202 26.74632672060407 28.033337309716234 23.68511532703768 100-101 21.53423830109326 26.015617001493574 28.641457017723237 23.80868767968993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5526.0 1 3309.0 2 1232.0 3 1376.0 4 1181.0 5 1196.5 6 1972.5 7 3741.0 8 5810.0 9 6657.5 10 5905.0 11 5096.5 12 5197.0 13 5584.5 14 6008.0 15 6462.0 16 7061.0 17 7455.5 18 7537.0 19 7799.0 20 8304.5 21 9200.5 22 10620.0 23 12591.5 24 14732.5 25 17544.5 26 22755.5 27 28642.5 28 32028.5 29 35812.5 30 41408.5 31 48048.0 32 55337.0 33 63309.0 34 71871.0 35 79054.0 36 89102.5 37 98698.0 38 103347.5 39 106288.0 40 109103.5 41 110864.5 42 110898.5 43 114899.0 44 120462.0 45 124800.0 46 132463.0 47 155105.0 48 203767.5 49 201122.5 50 150556.0 51 127126.5 52 118537.5 53 115115.5 54 110884.0 55 103236.0 56 94789.0 57 85604.0 58 76479.0 59 68684.0 60 58517.0 61 47580.0 62 37675.5 63 28176.0 64 20731.0 65 15418.0 66 11041.5 67 7910.0 68 6039.0 69 4595.5 70 3225.0 71 2010.0 72 1284.5 73 886.0 74 594.5 75 377.0 76 259.0 77 160.5 78 96.5 79 74.0 80 55.0 81 41.5 82 34.5 83 26.5 84 23.0 85 22.5 86 18.5 87 17.0 88 16.5 89 11.5 90 8.0 91 7.0 92 5.0 93 4.0 94 3.5 95 5.0 96 4.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28602167748893914 2 0.08811616186302434 3 0.09230577516708768 4 0.10672771327145955 5 0.10820482052609727 6 0.08387283556788327 7 0.09338003498864239 8 0.09021096851505601 9 0.09528684617190199 10-11 0.08830415733179643 12-13 0.09312489828102315 14-15 0.10158469437576642 16-17 0.09017068377174771 18-19 0.10126241642930002 20-21 0.10331693833802338 22-23 0.11611405846229375 24-25 0.129032032816489 26-27 0.13158339989268145 28-29 0.12371444669979327 30-31 0.11579178051582735 32-33 0.1104473379035927 34-35 0.3830139111275592 36-37 0.5869084252586415 38-39 0.5695457008927637 40-41 0.5318928941472714 42-43 0.5588299591727555 44-45 0.5803554403471578 46-47 0.5687802907699059 48-49 0.5912726057837074 50-51 0.5833230831042027 52-53 0.5861295868880143 54-55 0.5762195400341722 56-57 0.5483962106559054 58-59 0.543521756715601 60-61 0.4915275813523534 62-63 0.5652083768632364 64-65 0.5993966956842148 66-67 0.6121803875607158 68-69 0.6134426428510426 70-71 0.610233291634148 72-73 0.6014780740884771 74-75 0.5990072764989013 76-77 0.582463675246959 78-79 0.5594879433134577 80-81 0.5542240701878397 82-83 0.5688340037609836 84-85 0.5785291986505148 86-87 0.5681760196202814 88-89 0.5815236979030985 90-91 0.5957442122909289 92-93 0.6031968898029647 94-95 0.6024180514323375 96-97 0.5988327092778987 98-99 0.603613165483817 100-101 0.5809328550012435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3723494.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.533279901383246 #Duplication Level Percentage of deduplicated Percentage of total 1 68.85725954081683 29.975823528325712 2 15.232207314429857 13.262158890699444 3 6.048057909420255 7.898753934917002 4 3.0979277254379016 5.394518191429749 5 1.7751180272304072 3.863835496870629 6 1.1203812406051308 2.92643220861133 7 0.7729200092623458 2.3553420173218202 8 0.5427393872299999 1.8901780526231056 9 0.41053052300885395 1.6084566149585127 >10 1.94050844564828 14.721434215252785 >50 0.12435312485491251 3.7389126223306555 >100 0.07182294521686479 5.604204024258932 >500 0.0032103795638860027 0.9133745835945069 >1k 0.0020373562511373016 1.901009221977986 >5k 6.173806821628187E-4 1.780064494787632 >10k+ 3.0869034108140935E-4 2.1655019020402695 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26916 0.7228694339241584 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15387 0.41324089685655463 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14724 0.39543504031428545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12477 0.33508849483845016 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11068 0.29724769262418577 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9729 0.26128684509764216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8018 0.2153353812306398 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7597 0.20402879660877657 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6342 0.17032389470749784 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6294 0.1690347829216322 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6152 0.16522116055511302 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5580 0.14985924510688078 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5571 0.14961753664703098 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5545 0.14891926776302045 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5403 0.14510564539650123 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4479 0.12029024351858765 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4293 0.11529493534835827 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6856495538867527E-5 0.0 4 0.0 0.0 0.0 2.6856495538867527E-5 0.0 5 0.0 0.0 0.0 2.6856495538867527E-5 0.0 6 0.0 0.0 0.0 2.6856495538867527E-5 0.0 7 2.6856495538867527E-5 0.0 0.0 5.3712991077735054E-5 0.0 8 2.6856495538867527E-5 0.0 0.0 5.3712991077735054E-5 0.0 9 2.6856495538867527E-5 0.0 0.0 5.3712991077735054E-5 0.0 10-11 2.6856495538867527E-5 0.0 0.0 1.2085422992490387E-4 0.0 12-13 2.6856495538867527E-5 0.0 0.0 1.3428247769433762E-4 0.0 14-15 2.6856495538867527E-5 0.0 0.0 1.879954687720727E-4 0.0 16-17 2.6856495538867527E-5 0.0 0.0 2.81993203158109E-4 0.0 18-19 2.6856495538867527E-5 0.0 0.0 3.3570619423584406E-4 0.0 20-21 2.6856495538867527E-5 0.0 0.0 4.834169196996155E-4 0.0 22-23 2.6856495538867527E-5 0.0 0.0 7.519818750882908E-4 0.0 24-25 2.6856495538867527E-5 0.0 0.0 0.0011145445648630024 0.0 26-27 2.6856495538867527E-5 0.0 0.0 0.0018396699444124254 0.0 28-29 2.6856495538867527E-5 0.0 0.0 0.005411583851081807 0.0 30-31 2.6856495538867527E-5 0.0 0.0 0.014636790068682803 0.0 32-33 2.6856495538867527E-5 0.0 0.0 0.03381232788343422 0.0 34-35 2.6856495538867527E-5 0.0 0.0 0.061501374784006635 0.0 36-37 2.6856495538867527E-5 0.0 0.0 0.10447176764619467 0.0 38-39 2.6856495538867527E-5 0.0 0.0 0.16892735693947675 0.0 40-41 2.6856495538867527E-5 0.0 0.0 0.24219187676950737 0.0 42-43 2.6856495538867527E-5 0.0 0.0 0.31862546307312434 0.0 44-45 8.056948661660258E-5 0.0 0.0 0.4137511702717931 0.0 46-47 9.399773438603635E-5 0.0 0.0 0.5294220965576955 0.0 48-49 1.0742598215547011E-4 0.0 0.0 0.6488260757235005 0.0 50-51 1.0742598215547011E-4 0.0 0.0 0.7787309446450028 0.0 52-53 1.0742598215547011E-4 0.0 0.0 0.9103412010332231 0.0 54-55 1.0742598215547011E-4 0.0 0.0 1.060952428015192 0.0 56-57 1.0742598215547011E-4 0.0 0.0 1.2477393544880158 0.0 58-59 1.0742598215547011E-4 0.0 0.0 1.4544000876596015 0.0 60-61 1.0742598215547011E-4 0.0 0.0 1.6781146954983681 0.0 62-63 1.0742598215547011E-4 0.0 0.0 1.9087314226906233 0.0 64-65 1.0742598215547011E-4 0.0 0.0 2.1466531166694507 0.0 66-67 1.3428247769433762E-4 0.0 0.0 2.427196069068461 0.0 68-69 1.3428247769433762E-4 0.0 0.0 2.746304949061285 0.0 70-71 1.3428247769433762E-4 0.0 0.0 3.0901218049498667 0.0 72-73 1.3428247769433762E-4 0.0 0.0 3.4342877952804542 0.0 74-75 1.3428247769433762E-4 0.0 0.0 3.8088419103132702 0.0 76-77 1.3428247769433762E-4 0.0 0.0 4.20181958128575 0.0 78-79 1.3428247769433762E-4 0.0 0.0 4.673795633885807 0.0 80-81 2.1485196431094022E-4 0.0 0.0 5.185586441122236 0.0 82-83 2.4170845984980774E-4 1.3428247769433763E-5 0.0 5.690273705288635 0.0 84-85 2.551367076192415E-4 2.6856495538867527E-5 0.0 6.227900461233455 0.0 86-87 2.6856495538867523E-4 2.6856495538867527E-5 0.0 6.785696445327963 0.0 88-89 2.6856495538867523E-4 2.6856495538867527E-5 0.0 7.394962903122712 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15150 0.0 24.034866 1 TACATGG 15135 0.0 23.139788 2 TTTGCGT 1070 0.0 23.000095 3 ACATGGG 14995 0.0 22.18804 3 GAGTACT 13425 0.0 21.274027 12-13 GTACTTT 14010 0.0 20.11629 14-15 AGTACTT 13940 0.0 19.809103 12-13 CTTTGCG 1250 0.0 19.68808 2 CATGGGG 7620 0.0 18.819052 4 CATGGGA 7520 0.0 18.25115 4 GTATAGG 1005 0.0 17.455359 1 ACTTTTT 17010 0.0 16.917364 16-17 AGTACAT 11220 0.0 16.914595 2 TACTTTT 16630 0.0 16.392096 14-15 AGAGTAC 27690 0.0 15.99661 10-11 TTGCGTT 1450 0.0 15.993302 4 TATGACG 220 5.8651477E-4 15.058628 4 ACCGTGC 540 9.094947E-12 14.898078 8 TTAGCGC 260 1.25621E-4 14.56219 4 ATGGGAT 3385 0.0 14.54029 5 >>END_MODULE