FastQCFastQC Report
Fri 27 May 2016
SRR939020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723494
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT283330.7609250881027336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156050.4190956128840277No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT137760.369975082543439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131520.3532166293271857No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT106490.2859948209934003No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC92330.24796602331036388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85770.2303481622368668No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77550.20827212290391767No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC70320.18885487662931644No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67460.18117391890520035No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63920.17166671948444123No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG62350.16745024968483901No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA59850.16073612580012214No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52550.14113088405674884No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC51280.13772010912331267No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46310.12437243084049551No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA44800.12031710001412652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT9200.022.6946753
GTACATG144350.021.423331
TACATGG141200.021.373792
GAGTACT143350.020.62344212-13
ACATGGG143050.020.201733
GTACTTT148950.019.57727214-15
CATGGGG70650.019.276534
AGTACTT152050.018.92845512-13
CTTTGCG12300.016.5890852
ACTTTTT179300.016.46138216-17
AGTACAT110550.016.2682042
TACTTTT174900.016.12994414-15
AGAGTAC282900.015.53266310-11
TTGCGTT13750.015.5299094
TTAGCGC2154.8275891E-415.4496524
TAGTGCG1950.004493712414.6007694
CATGGGA75750.014.5333074
GGACCGA8550.014.4296136
GTGTACG3708.0324935E-714.1660921
ATGGGGA35200.014.1548655
Produced by FastQC (version 0.11.5)