##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939020_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3723494 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15621402908129 33.0 31.0 34.0 30.0 34.0 2 32.385890778929685 34.0 31.0 34.0 30.0 34.0 3 32.48278767200914 34.0 31.0 34.0 30.0 34.0 4 36.0509626173696 37.0 35.0 37.0 35.0 37.0 5 35.96979825937681 37.0 35.0 37.0 35.0 37.0 6 36.01554427105294 37.0 35.0 37.0 35.0 37.0 7 35.961293344369565 37.0 35.0 37.0 35.0 37.0 8 35.93130001015176 37.0 35.0 37.0 35.0 37.0 9 37.60354172720568 39.0 37.0 39.0 35.0 39.0 10-11 37.52460578693023 39.0 37.0 39.0 35.0 39.0 12-13 37.45358767866955 39.0 37.0 39.0 34.0 39.0 14-15 38.6857227378371 40.0 38.0 41.0 34.0 41.0 16-17 38.69115177841027 40.0 38.0 41.0 34.0 41.0 18-19 38.80955441313992 40.0 38.0 41.0 34.0 41.0 20-21 38.80686191517967 40.0 38.0 41.0 34.0 41.0 22-23 38.745340532306486 40.0 38.0 41.0 34.0 41.0 24-25 38.679983639022915 40.0 38.0 41.0 34.0 41.0 26-27 38.513349021107594 40.0 38.0 41.0 34.0 41.0 28-29 38.33950370270504 40.0 38.0 41.0 34.0 41.0 30-31 38.17819137079313 40.0 38.0 41.0 33.5 41.0 32-33 38.01326643738381 40.0 37.0 41.0 33.0 41.0 34-35 37.97605541998993 40.0 37.0 41.0 33.0 41.0 36-37 37.86754524110956 40.0 37.0 41.0 33.0 41.0 38-39 37.73929768652776 40.0 37.0 41.0 33.0 41.0 40-41 37.59878678467052 40.0 37.0 41.0 33.0 41.0 42-43 37.429531912767956 40.0 37.0 41.0 32.0 41.0 44-45 37.17041426681499 40.0 36.5 41.0 31.5 41.0 46-47 36.85348814849708 40.0 36.0 41.0 31.0 41.0 48-49 36.54351866821861 39.5 36.0 41.0 30.0 41.0 50-51 36.26984748733314 39.0 35.5 41.0 30.0 41.0 52-53 36.05812618470716 39.0 35.0 41.0 29.0 41.0 54-55 35.824395312574694 39.0 35.0 40.0 28.5 41.0 56-57 35.537088148926784 38.5 35.0 40.0 28.0 41.0 58-59 35.23608551537883 38.0 34.5 40.0 28.0 41.0 60-61 34.79927119528057 38.0 34.0 40.0 26.5 41.0 62-63 34.496090231379455 37.0 34.0 40.0 26.0 41.0 64-65 34.24898670442332 37.0 34.0 39.5 26.0 41.0 66-67 33.789967030966075 36.0 33.0 39.0 25.5 41.0 68-69 33.36286455678457 36.0 33.0 39.0 25.0 40.0 70-71 32.88457239356368 35.0 32.0 38.5 24.0 40.0 72-73 32.360141442419405 35.0 32.0 37.5 22.5 39.5 74-75 31.902645337954084 35.0 31.0 37.0 21.5 39.0 76-77 29.929554740789165 33.0 29.0 35.0 18.5 37.5 78-79 30.91572740549602 34.0 30.5 36.0 20.0 38.0 80-81 30.909329248281317 34.0 31.0 35.5 19.5 37.0 82-83 30.585058415563445 34.0 31.0 35.0 18.0 37.0 84-85 30.181931272079396 34.0 30.0 35.0 15.0 36.5 86-87 29.642925569371133 34.0 30.0 35.0 7.5 36.0 88-89 29.071884230241814 34.0 29.0 35.0 2.0 35.5 90-91 28.610354280146552 34.0 29.0 35.0 2.0 35.0 92-93 28.2687905499512 33.0 29.0 35.0 2.0 35.0 94-95 27.870133804432072 33.0 27.5 35.0 2.0 35.0 96-97 27.251574999180875 33.0 27.0 35.0 2.0 35.0 98-99 26.37940748662412 33.0 25.5 34.5 2.0 35.0 100-101 22.066759473763085 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14636703168196874 1101 2 0.19044660904334876 1101 3 0.22730778783025585 1101 4 0.15698335573847544 1101 5 0.17479997201093767 1101 6 0.1308113988048163 1101 7 0.120732515995563 1101 8 0.18015919139701708 1101 9 0.2513239272867551 1101 10-11 0.2809661925670639 1101 12-13 0.2488216117691593 1101 14-15 0.3184695327701874 1101 16-17 0.27438829149122057 1101 18-19 0.30390020153490127 1101 20-21 0.26304582030275014 1101 22-23 0.2964245317148553 1101 24-25 0.37418748363180043 1101 26-27 0.2575365296803298 1101 28-29 0.34796524440753274 1101 30-31 0.37494690198501957 1101 32-33 0.3282648461591009 1101 34-35 0.4596014580983905 1101 36-37 0.31096892273230736 1101 38-39 0.33697749137323996 1101 40-41 0.40041031604702226 1101 42-43 0.35329606930483237 1101 44-45 0.5056869621660169 1101 46-47 0.47189370484547766 1101 48-49 0.23514963278012857 1101 50-51 0.4212565784356386 1101 52-53 0.5215824754850686 1101 54-55 0.5815094174048241 1101 56-57 0.3990985968347047 1101 58-59 0.45033551926287885 1101 60-61 0.5213170075867097 1101 62-63 0.49336507012645825 1101 64-65 0.4540121783412516 1101 66-67 0.4908423591366855 1101 68-69 0.47288092830179096 1101 70-71 0.4647327677134072 1101 72-73 0.37209415493422426 1101 74-75 0.3132199426205844 1101 76-77 0.18376599668325255 1101 78-79 0.48022218661432703 1101 80-81 0.5947645682732556 1101 82-83 0.5723017970096542 1101 84-85 0.526739405694375 1101 86-87 0.7076385574226194 1101 88-89 0.7608497713938043 1101 90-91 0.854440974193384 1101 92-93 0.7912470005515644 1101 94-95 0.6826410336838364 1101 96-97 0.41074849891404597 1101 98-99 0.6375100952561503 1101 100-101 1.13334844905879 1102 1 0.16474511462246255 1102 2 0.09220243332428169 1102 3 0.09450735058218385 1102 4 0.12113926200341751 1102 5 0.15461140878076662 1102 6 0.08909178412967833 1102 7 0.16326855480132707 1102 8 0.1772009349236967 1102 9 0.23364270845775792 1102 10-11 0.2317638286946888 1102 12-13 0.22366276774374683 1102 14-15 0.23348456317259547 1102 16-17 0.264965253983533 1102 18-19 0.12724685727036444 1102 20-21 0.20620186320772405 1102 22-23 0.14810691198403703 1102 24-25 0.18969954211372908 1102 26-27 0.2372693984375971 1102 28-29 0.18983857910725987 1102 30-31 0.1326533992180643 1102 32-33 0.11580669216579764 1102 34-35 0.19027543500622812 1102 36-37 0.2690392240235582 1102 38-39 0.24773276909408537 1102 40-41 0.2140504062294255 1102 42-43 0.17337019651669294 1102 44-45 0.10128032146095478 1102 46-47 0.21997391253830756 1102 48-49 0.12253605076195839 1102 50-51 0.22165625049958493 1102 52-53 0.23333180936495523 1102 54-55 0.17473378032071452 1102 56-57 0.23134734999450757 1102 58-59 0.09983165918226433 1102 60-61 0.09752797978046601 1102 62-63 0.255242504026846 1102 64-65 0.17412558955940227 1102 66-67 0.19776659224455528 1102 68-69 0.19751712424358203 1102 70-71 0.27393205718529856 1102 72-73 0.2552635202413214 1102 74-75 0.2508506046414922 1102 76-77 0.22443177518977464 1102 78-79 0.16052381946258976 1102 80-81 0.2387182473058438 1102 82-83 0.26706848417232365 1102 84-85 0.20003311342136598 1102 86-87 0.2282336247178307 1102 88-89 0.37076812898074607 1102 90-91 0.33761887950185 1102 92-93 0.3652273243884139 1102 94-95 0.3563736974810645 1102 96-97 0.25288998965668696 1102 98-99 0.3350556988634459 1102 100-101 1.0194829028402985 1103 1 -0.0041700218613272 1103 2 0.04626296344166292 1103 3 0.0779091657524873 1103 4 0.05032768323859926 1103 5 0.10042528400418149 1103 6 0.007618458680504148 1103 7 0.013187756531728212 1103 8 0.0736273158161751 1103 9 0.09631918048771837 1103 10-11 0.18914501734165157 1103 12-13 0.08596463897647055 1103 14-15 0.08874934381857713 1103 16-17 0.053214841753771225 1103 18-19 0.11473513297391236 1103 20-21 0.08678939411723263 1103 22-23 0.1683033487865302 1103 24-25 0.0633139125483595 1103 26-27 0.0664543595378504 1103 28-29 0.16460224983258342 1103 30-31 0.21122960312875705 1103 32-33 0.12177933815156905 1103 34-35 0.15579168919596498 1103 36-37 0.11122609520067783 1103 38-39 0.17840801854303123 1103 40-41 0.1696055910562464 1103 42-43 0.1473156277174681 1103 44-45 0.16376187255705332 1103 46-47 0.3467970432107137 1103 48-49 0.33410851319580814 1103 50-51 0.25842611569026275 1103 52-53 0.30974456391658123 1103 54-55 0.2766775111123323 1103 56-57 0.22520231514394595 1103 58-59 0.12085902267067183 1103 60-61 0.10050101832678138 1103 62-63 0.1536248085673222 1103 64-65 0.1805565386538106 1103 66-67 0.20423848626886354 1103 68-69 0.20675856792720992 1103 70-71 0.18288169615728833 1103 72-73 0.1347599552725498 1103 74-75 0.15505849943362193 1103 76-77 0.1124200815814298 1103 78-79 0.3006895187332006 1103 80-81 0.2314981402970524 1103 82-83 0.3537084501311867 1103 84-85 0.30046764869846854 1103 86-87 0.24583578417155394 1103 88-89 0.28892728777251264 1103 90-91 0.496525699304204 1103 92-93 0.38457162058255534 1103 94-95 0.3253787562807027 1103 96-97 0.194063000109999 1103 98-99 0.4051158928534413 1103 100-101 1.024880589740107 1104 1 0.022423401392430264 1104 2 0.03668926238007941 1104 3 0.06618186237479762 1104 4 0.05428579429974434 1104 5 0.04623588720827598 1104 6 0.0389723603224752 1104 7 -0.051055436981826574 1104 8 0.022746334971998294 1104 9 0.027966839177075542 1104 10-11 0.03786954195414438 1104 12-13 0.08647495015712536 1104 14-15 0.04720316104515376 1104 16-17 0.048316696692594974 1104 18-19 0.0493540530604335 1104 20-21 0.0072207148022371825 1104 22-23 -0.027476814014228523 1104 24-25 -0.018755336527569 1104 26-27 -0.10370635025467578 1104 28-29 -0.06929678026951791 1104 30-31 -0.10413154435138239 1104 32-33 -0.0037989579594253087 1104 34-35 0.020491762702697258 1104 36-37 0.011739497441752178 1104 38-39 0.16113504200802709 1104 40-41 0.05210702435152825 1104 42-43 0.03287598422958382 1104 44-45 0.05724666948706414 1104 46-47 0.14793616439934 1104 48-49 -0.06723595907635627 1104 50-51 -0.14188767600808205 1104 52-53 -0.08535036475719693 1104 54-55 0.023947626372084585 1104 56-57 -0.06849855345221556 1104 58-59 0.041273416119977924 1104 60-61 0.20330519073500852 1104 62-63 0.15118315703499974 1104 64-65 0.11098490484111068 1104 66-67 0.25258263054517016 1104 68-69 0.2747192522552737 1104 70-71 0.09995578176150843 1104 72-73 0.05725181936434609 1104 74-75 0.03463605941429648 1104 76-77 0.06899955499393329 1104 78-79 0.1159317666929951 1104 80-81 0.22459669368894808 1104 82-83 0.3102268383018121 1104 84-85 0.27691282780130066 1104 86-87 0.21313588108202453 1104 88-89 0.2262712474847106 1104 90-91 0.30539390262041977 1104 92-93 0.21117138950534553 1104 94-95 0.3693101664674465 1104 96-97 0.3665690724491526 1104 98-99 0.4530404542969748 1104 100-101 0.9505846216849889 1105 1 -0.12876799983478548 1105 2 -0.10213032227381547 1105 3 -0.04344308888794757 1105 4 -0.01861004998753657 1105 5 0.027514697493835172 1105 6 0.022044780625456895 1105 7 -0.052019871722492894 1105 8 -0.0014911812786877476 1105 9 -0.0776275064716927 1105 10-11 -0.11373431373064591 1105 12-13 -0.11901269694840977 1105 14-15 -0.04064410795012918 1105 16-17 0.04044407444850151 1105 18-19 -0.03538833178038914 1105 20-21 -0.01063092721368264 1105 22-23 -0.08272564362878398 1105 24-25 -0.05702199281802933 1105 26-27 -0.014623298020580933 1105 28-29 -0.018930327460807916 1105 30-31 -0.05912332567980627 1105 32-33 -0.11308749870970303 1105 34-35 0.05890600350167574 1105 36-37 -9.04122022404863E-5 1105 38-39 -0.005530373719281556 1105 40-41 0.041395059458942285 1105 42-43 0.07555710016756478 1105 44-45 0.028494039057868292 1105 46-47 0.09930876748431672 1105 48-49 -0.13749534991102763 1105 50-51 0.027574023718280216 1105 52-53 0.06655304481628121 1105 54-55 0.0807806982590975 1105 56-57 0.0430503688767061 1105 58-59 -0.03395431080600986 1105 60-61 -0.031266914923804734 1105 62-63 -0.008400770000740465 1105 64-65 -0.11642634989978973 1105 66-67 0.009556768888266731 1105 68-69 -0.04846814649270215 1105 70-71 0.07690139078660962 1105 72-73 0.016521497265905793 1105 74-75 0.07086007658087112 1105 76-77 0.07691547290755452 1105 78-79 0.2544689419112842 1105 80-81 0.10664001227041453 1105 82-83 0.11269398040444045 1105 84-85 -0.004629627829526584 1105 86-87 -0.00449849251931056 1105 88-89 0.14444330115712134 1105 90-91 0.3081189620828333 1105 92-93 0.34010360215502544 1105 94-95 0.2829542278698689 1105 96-97 0.2569577498415043 1105 98-99 0.36551912742691783 1105 100-101 0.8835581848593357 1106 1 -0.0633548776809647 1106 2 -0.11315161892164127 1106 3 -0.12066160893976274 1106 4 -0.04784157352870011 1106 5 -0.01739121989508874 1106 6 0.012577187282111879 1106 7 0.007795797711104058 1106 8 0.007742517141849703 1106 9 -0.05072081452046007 1106 10-11 0.027939618432192503 1106 12-13 0.013537234657363229 1106 14-15 -0.12319833208098885 1106 16-17 -0.11907836782658165 1106 18-19 -0.1264325982069323 1106 20-21 -0.11222393097011718 1106 22-23 0.05778663585466148 1106 24-25 0.014757611773639212 1106 26-27 -0.05333348008087313 1106 28-29 0.03326639610247639 1106 30-31 0.10952578805684965 1106 32-33 0.038446458979358056 1106 34-35 0.04873939629442958 1106 36-37 -0.061020039591980435 1106 38-39 0.03201281385262433 1106 40-41 0.17776343060783262 1106 42-43 0.17683779887618556 1106 44-45 0.028998840008711113 1106 46-47 0.032663786018481744 1106 48-49 -0.07224152813285656 1106 50-51 -0.06574623490158871 1106 52-53 0.08053351287320965 1106 54-55 0.08195494234542622 1106 56-57 0.06882650510809185 1106 58-59 0.10454334356749939 1106 60-61 0.11619868287231583 1106 62-63 0.12184016466321879 1106 64-65 0.03315593971402109 1106 66-67 0.11836617505080937 1106 68-69 0.05526234398075047 1106 70-71 -0.04405128269340963 1106 72-73 0.21703621871917989 1106 74-75 0.31778119141640815 1106 76-77 -0.014700367082372878 1106 78-79 0.2495788973262556 1106 80-81 0.3515304765690068 1106 82-83 0.3002115082152379 1106 84-85 0.22203820894768356 1106 86-87 0.21238105159544673 1106 88-89 0.3227278936629432 1106 90-91 0.4552436379971887 1106 92-93 0.3631052409457176 1106 94-95 0.5602673742526072 1106 96-97 0.5210001256492163 1106 98-99 0.6078275901878314 1106 100-101 1.1310342754945708 1107 1 -0.11173647968582401 1107 2 -0.054347752403202776 1107 3 -0.09345239863657895 1107 4 -0.10816755193498295 1107 5 -0.11997832019468291 1107 6 -0.11853660367717112 1107 7 -0.1269116696439454 1107 8 -0.09043727973403293 1107 9 -0.017159359111666106 1107 10-11 -0.022781679451775005 1107 12-13 -0.049429545693492116 1107 14-15 0.024389616139323778 1107 16-17 -0.014568560210364012 1107 18-19 -0.07824281470934835 1107 20-21 -0.04753116090736853 1107 22-23 -0.0767275055785106 1107 24-25 -0.16945259719170735 1107 26-27 -0.07491570012572168 1107 28-29 -0.05068303013783293 1107 30-31 -0.08807968385813325 1107 32-33 -0.04089875780570651 1107 34-35 -0.14997986657983375 1107 36-37 -0.15090378024130047 1107 38-39 -0.13047442023002276 1107 40-41 -0.30160533953028335 1107 42-43 -0.34191330813140297 1107 44-45 -0.3007518713025519 1107 46-47 -0.38469993428073224 1107 48-49 -0.247482620254722 1107 50-51 -0.2437489105624948 1107 52-53 -0.12537065324386987 1107 54-55 -0.19616380423934032 1107 56-57 -0.18912925227353128 1107 58-59 -0.2350752805398315 1107 60-61 -0.24700494999685674 1107 62-63 -0.28973263404040495 1107 64-65 -0.3508517104150499 1107 66-67 -0.2105710976593329 1107 68-69 -0.303032393258043 1107 70-71 -0.35542585637772817 1107 72-73 -0.3416137030836879 1107 74-75 -0.41596529913398683 1107 76-77 -0.6621353127212295 1107 78-79 -0.46420584386950026 1107 80-81 -0.21212293113011427 1107 82-83 -0.2494459318179274 1107 84-85 -0.3078059620685032 1107 86-87 -0.1933243242218552 1107 88-89 -0.12285896168178212 1107 90-91 0.006049727952156303 1107 92-93 0.0900122146224902 1107 94-95 0.10584461982928772 1107 96-97 0.11516077835973704 1107 98-99 0.06344704337318419 1107 100-101 0.6137947284726586 1108 1 0.027831068863619635 1108 2 -0.009459230646783112 1108 3 0.011134003694706962 1108 4 -0.02029531403314877 1108 5 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quality scores pass #Quality Count 2 2.0 3 7.0 4 25.0 5 80.0 6 181.0 7 464.0 8 1092.0 9 2045.0 10 3430.0 11 5204.0 12 7581.0 13 10699.0 14 12821.0 15 17221.0 16 23038.0 17 32473.0 18 31644.0 19 21323.0 20 19682.0 21 20689.0 22 22373.0 23 24327.0 24 28674.0 25 34392.0 26 41006.0 27 50218.0 28 60989.0 29 74935.0 30 90492.0 31 111355.0 32 137981.0 33 175197.0 34 235981.0 35 338697.0 36 533602.0 37 845719.0 38 657651.0 39 50203.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7158269258721 19.927934769154763 14.932986939862314 22.423251365110815 2 15.30224031514486 21.70840076551755 39.30603889787388 23.68332002146371 3 19.922255816714085 25.206083318517503 30.581921174037074 24.28973969073134 4 12.893185808812904 17.206473274832724 37.92325165556867 31.9770892607857 5 12.8880344903433 38.41672716119906 34.636523872713596 14.058714475744042 6 30.530302989611368 37.120672142885155 17.3242658642662 15.024759003237282 7 27.061491169315705 33.72778363547786 21.353250468511565 17.85747472669487 8 25.4403256726075 34.71411529063831 19.991733570673137 19.85382546608105 9 25.657997568950027 17.53841955969313 21.474212124418624 35.329370746938224 10-11 24.739693416989528 26.571279556244754 27.3994130244335 21.28961400233222 12-13 26.388171969660757 24.2513617586063 27.61570181125577 21.744764460477175 14-15 22.857878348567382 25.64962462313258 27.12132109453094 24.37117593376909 16-17 21.246428338347112 28.72961510788372 27.78145123538738 22.242505318381795 18-19 21.18020063950687 28.25148905839515 29.49317227313915 21.075138028958822 20-21 22.43003480064692 26.530941099945377 29.259426764216624 21.779597335191088 22-23 21.76239574979844 26.549821216309198 29.3628511285368 22.324931905355562 24-25 22.341932067031664 26.728886900314595 29.310185540785078 21.61899549186866 26-27 21.977355140091536 26.275576112114052 30.263080858999636 21.483987888794772 28-29 21.29959119042491 27.630808589996388 29.609501183565754 21.46009903601295 30-31 21.798880298987992 26.760107576378527 29.323143799882583 22.11786832475089 32-33 21.732155335821677 26.869816360654802 29.46888057292425 21.929147730599272 34-35 22.469564488745586 26.667764228204526 29.31133877306675 21.55133250998313 36-37 22.21730415474938 26.31629554207325 29.95457173913102 21.511828564046354 38-39 21.823170177313838 27.062784722611134 29.6761926403255 21.437852459749536 40-41 21.932748339724217 27.09136334307129 29.37449013368821 21.601398183516288 42-43 21.460032072207856 27.2710182133723 28.83363193448697 22.435317779932877 44-45 21.938961108544067 26.82197574123229 28.797816342970695 22.44124680725295 46-47 22.52988061718541 27.30628089176696 28.376810535509218 21.787027955538417 48-49 22.229346678551604 27.20303930649841 28.49484125204345 22.07277276290654 50-51 21.588421339715193 27.603777175084375 28.943642765532857 21.86415871966758 52-53 22.09448974538431 28.002287636290003 27.903442304459197 21.999780313866495 54-55 21.562825131449117 27.416856318285998 28.315488087264274 22.70483046300061 56-57 22.19737697979371 27.418561705752715 27.965937369578143 22.418123944875433 58-59 22.757932514092634 27.119245836263495 27.997212828776025 22.12560882086785 60-61 22.08507619440117 27.460802999191465 28.794945269831757 21.65917553657561 62-63 21.827025906312727 27.34877510209497 28.72679531644203 22.097403675150275 64-65 21.56481776530916 27.989745618448758 28.33261021976341 22.11282639647867 66-67 21.868018892197057 27.33821286863069 27.79639108115555 22.997377158016697 68-69 21.55346011607577 27.526111676599264 28.168355521656107 22.75207268566886 70-71 22.320621437821572 27.33659568136809 27.761035736864354 22.581747143945982 72-73 22.011113754983892 26.8694672262128 28.418321608682596 22.70109741012071 74-75 21.433376822951775 28.24556720112883 27.704113394569724 22.616942581349665 76-77 21.683869898205792 27.174446024841437 27.850734633627034 23.290949443325736 78-79 21.785832166641466 27.20776013692527 28.108272586129523 22.898135110303745 80-81 22.08094064338495 27.27359034283391 27.752925075211614 22.892543938569528 82-83 21.82525337760716 27.01145751812679 28.346990756531365 22.81629834773468 84-85 21.53839914875652 27.24626385862311 28.035428551784964 23.17990844083541 86-87 21.387468327346333 27.4115697357834 27.974882699727843 23.226079237142425 88-89 21.496246543241465 27.201727411516895 27.596043699982964 23.705982345258676 90-91 21.621947185492147 26.62359439665249 27.931379002066258 23.82307941578911 92-93 21.846684861047176 27.122777692135397 27.837845314105515 23.192692132711908 94-95 21.565712204719546 26.90928198084917 27.879392312704145 23.64561350172714 96-97 20.80281865276068 26.82590565119038 28.40759762168788 23.963678074361063 98-99 21.175353042062106 26.993114531673747 27.561599400992726 24.269933025271424 100-101 21.18919631705689 26.259775944043888 28.244540504276973 24.306487234622253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2348.0 1 1612.5 2 994.0 3 1131.5 4 975.5 5 1002.0 6 1745.0 7 3386.0 8 5198.0 9 5864.5 10 5113.5 11 4298.0 12 4380.5 13 4703.5 14 5020.0 15 5282.5 16 5498.0 17 5774.5 18 6254.0 19 6879.5 20 7616.5 21 8659.0 22 10049.0 23 12195.5 24 14661.5 25 17640.5 26 22758.5 27 28653.5 28 31977.5 29 35875.5 30 41921.0 31 48611.5 32 56018.0 33 63709.5 34 72001.5 35 79447.0 36 89569.0 37 98857.5 38 103401.5 39 106686.5 40 109247.0 41 110977.0 42 111149.5 43 115191.0 44 121356.0 45 126189.5 46 132761.5 47 155845.0 48 204452.5 49 200055.5 50 152663.5 51 132142.5 52 122373.0 53 117063.0 54 112176.0 55 104459.0 56 95297.0 57 86004.5 58 77261.0 59 68997.0 60 58457.0 61 47788.5 62 37504.0 63 27492.5 64 20104.5 65 14802.5 66 10288.5 67 7474.0 68 5676.5 69 4107.5 70 2862.5 71 1727.5 72 1068.5 73 743.5 74 494.5 75 300.5 76 168.0 77 75.0 78 32.5 79 23.0 80 14.0 81 11.0 82 6.5 83 1.5 84 3.0 85 2.5 86 1.0 87 1.0 88 0.5 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4078695977487811 2 0.0 3 0.0 4 0.0 5 4.56560424160748E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.66833839399231E-4 16-17 0.002336515111881475 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023767998551897764 36-37 0.007009545335644425 38-39 0.012421129186726231 40-41 0.002457369341806379 42-43 5.63986406316218E-4 44-45 4.162756808524467E-4 46-47 2.685649553886753E-4 48-49 3.4913444200527783E-4 50-51 6.311276451633869E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.477107254637714E-4 60-61 0.002430512846267511 62-63 0.0 64-65 3.357061942358441E-4 66-67 0.0013965377680211113 68-69 0.0017188157144875215 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12535269292766418 78-79 8.056948661660257E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1498189603635725 88-89 0.44164164088890706 90-91 0.34044636569845416 92-93 0.0 94-95 0.0 96-97 3.4913444200527783E-4 98-99 0.0 100-101 1.2085422992490387E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3723494.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.081064348075174 #Duplication Level Percentage of deduplicated Percentage of total 1 67.22316722187375 28.288224255450867 2 15.853014366956081 13.342234353736778 3 6.469300133216771 8.167051055787185 4 3.2700155619570648 5.504229411276897 5 1.9237044428653334 4.04757652234471 6 1.178786789910816 2.9762761655338807 7 0.8017047451039677 2.361561227681409 8 0.57386943869149 1.9319229421576292 9 0.4245137497096377 1.6077591376356546 >10 2.0684020136875283 15.173637779229757 >50 0.13052308591644468 3.8189151232626886 >100 0.07648733036083293 5.836266307623168 >500 0.003255559881130612 0.9107911144350488 >1k 0.002298042260618688 2.0081673429805167 >5k 6.3834507239408E-4 1.8357117617459344 >10k+ 3.1917253619704E-4 2.1896754991179117 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28333 0.7609250881027336 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15605 0.4190956128840277 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13776 0.369975082543439 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13152 0.3532166293271857 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10649 0.2859948209934003 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9233 0.24796602331036388 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8577 0.2303481622368668 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7755 0.20827212290391767 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7032 0.18885487662931644 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6746 0.18117391890520035 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6392 0.17166671948444123 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6235 0.16745024968483901 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5985 0.16073612580012214 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5255 0.14113088405674884 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5128 0.13772010912331267 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4631 0.12437243084049551 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4480 0.12031710001412652 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.6856495538867527E-5 0.0 8 0.0 0.0 0.0 2.6856495538867527E-5 0.0 9 0.0 0.0 0.0 5.3712991077735054E-5 0.0 10-11 0.0 0.0 0.0 6.714123884716882E-5 0.0 12-13 2.6856495538867527E-5 0.0 0.0 8.056948661660258E-5 0.0 14-15 2.6856495538867527E-5 0.0 0.0 1.0742598215547011E-4 0.0 16-17 2.6856495538867527E-5 0.0 0.0 1.7456722100263892E-4 0.0 18-19 4.028474330830129E-5 0.0 0.0 2.2828021208037396E-4 0.0 20-21 5.3712991077735054E-5 0.0 0.0 3.759909375441454E-4 0.0 22-23 5.3712991077735054E-5 0.0 0.0 6.445558929328206E-4 0.0 24-25 8.056948661660258E-5 0.0 0.0 0.0010339750782463997 0.0 26-27 8.056948661660258E-5 0.0 0.0 0.001759100457795823 0.0 28-29 8.056948661660258E-5 0.0 0.0 0.005357870860004071 0.0 30-31 8.056948661660258E-5 0.0 0.0 0.0145965053253745 0.0 32-33 8.056948661660258E-5 0.0 0.0 0.03401375159997572 0.0 34-35 1.074259821554701E-4 0.0 0.0 0.062172787172478323 0.0 36-37 1.3428247769433762E-4 1.3428247769433763E-5 0.0 0.10586830541421578 0.0 38-39 1.879954687720727E-4 2.6856495538867527E-5 0.0 0.1713310132902054 0.0 40-41 1.879954687720727E-4 2.6856495538867527E-5 0.0 0.2460726403748737 0.0 42-43 2.0142371654150647E-4 2.6856495538867527E-5 0.0 0.3238087667121258 0.0 44-45 2.1485196431094022E-4 2.6856495538867527E-5 0.0 0.42037129642212395 0.0 46-47 2.2828021208037396E-4 2.6856495538867527E-5 0.0 0.5375998994492808 0.0 48-49 2.551367076192415E-4 2.6856495538867527E-5 0.0 0.6584004163831069 0.0 50-51 2.6856495538867523E-4 2.6856495538867527E-5 0.0 0.7892855473917777 0.0 52-53 2.6856495538867523E-4 2.6856495538867527E-5 0.0 0.9220909178314777 0.0 54-55 2.6856495538867523E-4 2.6856495538867527E-5 0.0 1.0736286939095376 0.0 56-57 3.2227794646641034E-4 2.6856495538867527E-5 0.0 1.2613421694784521 0.0 58-59 3.2227794646641034E-4 2.6856495538867527E-5 0.0 1.4693860121702895 0.0 60-61 3.2227794646641034E-4 2.6856495538867527E-5 0.0 1.6944165882904607 0.0 62-63 3.2227794646641034E-4 2.6856495538867527E-5 0.0 1.9261747165431178 0.0 64-65 3.2227794646641034E-4 2.6856495538867527E-5 0.0 2.1651303856001918 0.0 66-67 3.4913444200527783E-4 2.6856495538867527E-5 0.0 2.447190729997148 0.0 68-69 3.4913444200527783E-4 2.6856495538867527E-5 0.0 2.767320156820449 0.0 70-71 3.4913444200527783E-4 2.6856495538867527E-5 0.0 3.1124664092382046 0.0 72-73 3.4913444200527783E-4 2.6856495538867527E-5 0.0 3.4600431745022284 0.0 74-75 3.4913444200527783E-4 2.6856495538867527E-5 0.0 3.8385451943792575 0.0 76-77 3.4913444200527783E-4 2.6856495538867527E-5 0.0 4.2349739250284815 0.0 78-79 3.4913444200527783E-4 2.6856495538867527E-5 0.0 4.709393918722576 0.0 80-81 4.2970392862188043E-4 2.6856495538867527E-5 0.0 5.222070990311788 0.0 82-83 4.2970392862188043E-4 2.6856495538867527E-5 0.0 5.727349097380042 2.6856495538867527E-5 84-85 4.2970392862188043E-4 2.6856495538867527E-5 0.0 6.265499554987869 2.6856495538867527E-5 86-87 4.4313217639131415E-4 2.6856495538867527E-5 0.0 6.823644673524383 2.6856495538867527E-5 88-89 4.565604241607479E-4 2.6856495538867527E-5 0.0 7.434065960627303 2.6856495538867527E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 920 0.0 22.694675 3 GTACATG 14435 0.0 21.42333 1 TACATGG 14120 0.0 21.37379 2 GAGTACT 14335 0.0 20.623442 12-13 ACATGGG 14305 0.0 20.20173 3 GTACTTT 14895 0.0 19.577272 14-15 CATGGGG 7065 0.0 19.27653 4 AGTACTT 15205 0.0 18.928455 12-13 CTTTGCG 1230 0.0 16.589085 2 ACTTTTT 17930 0.0 16.461382 16-17 AGTACAT 11055 0.0 16.268204 2 TACTTTT 17490 0.0 16.129944 14-15 AGAGTAC 28290 0.0 15.532663 10-11 TTGCGTT 1375 0.0 15.529909 4 TTAGCGC 215 4.8275891E-4 15.449652 4 TAGTGCG 195 0.0044937124 14.600769 4 CATGGGA 7575 0.0 14.533307 4 GGACCGA 855 0.0 14.429613 6 GTGTACG 370 8.0324935E-7 14.166092 1 ATGGGGA 3520 0.0 14.154865 5 >>END_MODULE