FastQCFastQC Report
Fri 27 May 2016
SRR939019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246331
Sequences flagged as poor quality0
Sequence length101
%GC16

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2777711.276290844432896No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227539.236758670244509No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161876.57123951106438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109954.4635064202231955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96133.9024726891865007No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48321.9615882694423357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45241.8365532555788755No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32781.3307297904039685No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32241.3088080671941413No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20830.845610174927232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.7481802939946657No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17450.7083964259472011No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16800.6820091665279644No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.6592755276436989No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15520.6300465633639291No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14990.6085307979913206No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.553726489966752No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.46969321766241356No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11130.4518310728247764No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10870.44127616905708167No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10850.44046425338264367No Hit
GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10440.4238199820566636No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.4092054999167786No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9860.40027442749796005No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9490.3852539875208561No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9380.3807884513114468No Hit
GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.37551099942759947No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8750.35521310756664815No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA8600.3491237400083627No Hit
GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8100.3288258481474114No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7540.3060922092631459No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7420.3012207152165176No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7380.2995968838676415No Hit
GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.29391347414657515No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7170.29107176928604195No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6980.28335857037888046No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.2756453714717189No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.2744274979600619No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6650.26996196175065257No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.26021897365739594No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.25737726879686273No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.2561593952852057No Hit
GAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.2520998169130154No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5820.23626746126147335No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5260.21353382237720792No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5080.2062265813072654No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4930.20013721374898003No Hit
GCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.19891934023732297No Hit
GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.1903942256557234No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.1826810267485619No Hit
GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.17943336405080967No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4370.17740357486471456No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.1749678278414004No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG4290.17415591216696233No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG4200.1705022916319911No Hit
GTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.168878460283115No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG4090.1660367554225818No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.1660367554225818No Hit
GTACATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.16035334570151544No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT3910.15872951435263932No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT3760.15264014679435392No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.14898652625938272No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA3660.1485805684221637No Hit
GTACATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.14655077923606852No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.1412733273522212No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3470.14086736951500217No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3460.14046141167778314No Hit
ACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.1396494960033451No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3430.13924353816612606No Hit
GTACATGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.13396608628227874No Hit
GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3300.13396608628227874No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3290.1335601284450597No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3160.12828267656121237No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3110.12625288737511722No Hit
ACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.12381714035180305No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3040.12341118251458404No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.12219330900292695No Hit
GTACATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.12219330900292695No Hit
GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.12219330900292695No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.1213813933284889No Hit
GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.12097543549126988No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2900.11772777279351765No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2890.11732181495629863No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.11569798360742253No Hit
GTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAA2840.1152920257702035No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC2760.1120443630724513No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2680.10879670037469907No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA2630.10676691118860396No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG2620.10636095335138493No Hit
GAGTACATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.10554903767694687No Hit
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2590.10514307983972786No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2550.10351924849085173No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT2510.10189541714197563No Hit
GTACATGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.1014894593047566No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.1014894593047566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT156.683771E-494.741214
AGCACAT301.31818E-463.1479877
GGATGGG250.005075135356.8331878
ATGGGTT1150.053.549385
GCATCAA558.319348E-751.677032
GGCATCA601.4945927E-647.4670261
CTTGGTA252.8739738E-547.3609916-17
GCACTTT205.463535E-447.3609914-15
CATCAAC751.386361E-744.212573
TATGGGA652.6299476E-643.7267154
CGCAGAA2600.040.074689
TTGGTAT308.428137E-539.4674916-17
GGAGTGA608.415852E-539.467498
TGGGAGT853.709465E-739.0031666
TGGGTGG1600.038.4808046
GGGTGAG757.040342E-637.8887947
GGTGAGA757.040342E-637.8887948
GAGCACT250.001638338137.8887912-13
TGGGTTG1251.382432E-1037.8887866
TGGGAAT1302.1100277E-1036.431536
Produced by FastQC (version 0.11.5)