##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939019_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 246331 Sequences flagged as poor quality 0 Sequence length 101 %GC 16 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.828726388477293 33.0 31.0 34.0 30.0 34.0 2 31.706975573516935 33.0 31.0 34.0 30.0 34.0 3 31.604682317694483 33.0 31.0 34.0 28.0 34.0 4 35.24796310655175 37.0 35.0 37.0 33.0 37.0 5 35.41393490872038 37.0 35.0 37.0 33.0 37.0 6 35.60523847993147 37.0 35.0 37.0 35.0 37.0 7 35.612781176547 37.0 35.0 37.0 35.0 37.0 8 35.60565661650381 37.0 36.0 37.0 35.0 37.0 9 36.97072638035814 39.0 37.0 39.0 34.0 39.0 10-11 36.92335921991142 39.0 37.0 39.0 33.0 39.0 12-13 36.740075345774585 39.0 37.0 39.0 32.5 39.0 14-15 37.64103381222826 40.0 37.5 41.0 32.5 41.0 16-17 37.60405308304679 39.5 37.0 41.0 33.0 41.0 18-19 37.3335857037888 39.0 36.0 41.0 34.0 41.0 20-21 37.27730573902595 39.0 35.0 41.0 33.0 41.0 22-23 37.0846665665304 39.0 35.0 41.0 33.0 41.0 24-25 36.92619280561521 39.0 35.0 41.0 33.0 41.0 26-27 36.580676000990536 37.5 35.0 41.0 33.0 41.0 28-29 36.2013753851525 36.5 35.0 40.5 33.0 41.0 30-31 35.54632790838343 35.0 35.0 39.5 33.0 41.0 32-33 34.846079056229215 35.0 35.0 38.0 33.0 41.0 34-35 34.403388124109426 35.0 35.0 36.5 31.5 41.0 36-37 34.018290430355904 35.0 35.0 35.0 31.0 40.0 38-39 33.7458399470631 35.0 35.0 35.0 31.0 40.0 40-41 33.55711217832916 35.0 35.0 35.0 31.0 40.0 42-43 33.38234935919556 35.0 35.0 35.0 29.5 40.0 44-45 32.85643301086749 35.0 33.0 35.0 22.5 40.0 46-47 32.46720672590945 35.0 33.0 35.0 22.5 40.0 48-49 32.25731637512128 35.0 33.0 35.0 21.0 40.0 50-51 31.179898185774427 34.0 30.0 35.0 20.0 39.0 52-53 31.631855105528743 35.0 33.0 35.0 20.0 39.0 54-55 32.161693006564334 35.0 33.0 35.0 21.0 40.0 56-57 32.1218462150521 35.0 33.0 35.0 20.5 40.0 58-59 32.02595288453341 35.0 33.0 35.0 21.0 40.0 60-61 31.822949608453666 35.0 33.0 35.0 20.0 39.5 62-63 31.50503184739233 35.0 33.0 35.0 19.0 39.0 64-65 31.244922482350983 35.0 33.0 35.0 12.0 39.0 66-67 30.928821382611204 35.0 33.0 35.0 2.0 38.0 68-69 30.62293418205585 35.0 33.0 35.0 2.0 37.0 70-71 30.264674360920875 35.0 33.0 35.0 2.0 37.0 72-73 29.907798856010814 35.0 31.5 35.0 2.0 36.0 74-75 29.575463502360645 35.0 31.0 35.0 2.0 35.0 76-77 29.255932058896363 35.0 31.0 35.0 2.0 35.0 78-79 29.126853704974202 35.0 31.0 35.0 2.0 35.0 80-81 28.81983388205301 35.0 31.0 35.0 2.0 35.0 82-83 28.4554481571544 35.0 30.5 35.0 2.0 35.0 84-85 28.104966488180537 35.0 29.0 35.0 2.0 35.0 86-87 27.662797211881575 35.0 29.0 35.0 2.0 35.0 88-89 27.176315607860968 34.0 27.0 35.0 2.0 35.0 90-91 26.731389877847285 34.0 26.0 35.0 2.0 35.0 92-93 26.21358050752849 33.0 24.0 35.0 2.0 35.0 94-95 25.76120545120184 33.0 20.0 35.0 2.0 35.0 96-97 25.212074403952407 33.0 8.5 35.0 2.0 35.0 98-99 24.551471394181 33.0 2.0 35.0 2.0 35.0 100-101 22.1616402320455 29.0 2.0 33.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13743443034107017 1101 2 0.5864184163272661 1101 3 0.15782732670742305 1101 4 -0.11639255614643673 1101 5 -0.01445774584894366 1101 6 0.09660551456221356 1101 7 0.18334435451506437 1101 8 -0.01395393325996963 1101 9 0.1093525171811578 1101 10-11 0.23517711800038654 1101 12-13 -0.19577501982762868 1101 14-15 0.2867299917185875 1101 16-17 0.079264113387012 1101 18-19 0.5189536527305663 1101 20-21 0.48365824220014986 1101 22-23 0.6657274596795375 1101 24-25 0.7912370873698151 1101 26-27 0.6789273578774484 1101 28-29 0.4671501843187187 1101 30-31 0.4834712525812961 1101 32-33 0.2632434845491076 1101 34-35 0.5670128176226328 1101 36-37 0.5351647212870674 1101 38-39 0.8105553555834746 1101 40-41 0.5867521867321344 1101 42-43 0.8443741058953904 1101 44-45 0.7101021155540579 1101 46-47 0.6541506622061917 1101 48-49 0.61745701815871 1101 50-51 1.0120288452709083 1101 52-53 1.104937230462152 1101 54-55 0.8478943115567859 1101 56-57 0.40199138047614014 1101 58-59 0.7054816161658977 1101 60-61 0.6416457190745177 1101 62-63 0.3836901668380861 1101 64-65 0.22489028509601283 1101 66-67 -0.01442220224129187 1101 68-69 0.09112903082606039 1101 70-71 0.36931479804726663 1101 72-73 0.46716155021285033 1101 74-75 0.6805295298157858 1101 76-77 0.4374941000032777 1101 78-79 0.30731058925859855 1101 80-81 0.24807789791396573 1101 82-83 0.5760879408080051 1101 84-85 0.38273686863468726 1101 86-87 0.24427362704364342 1101 88-89 0.301424981753847 1101 90-91 0.3223080179916238 1101 92-93 0.7036897750244826 1101 94-95 0.4980210462713899 1101 96-97 0.38828918378940713 1101 98-99 0.18741132779747716 1101 100-101 0.7532791558850427 1102 1 -0.27893705702525295 1102 2 -0.27972198781536406 1102 3 -0.20052034390621287 1102 4 -0.18490788860265894 1102 5 -0.13151742266978061 1102 6 -0.022427646723755856 1102 7 -0.0953217723951667 1102 8 0.05594452506070979 1102 9 -0.31778036410616295 1102 10-11 -0.1646137263810843 1102 12-13 0.23203255700332903 1102 14-15 0.03798661216860211 1102 16-17 0.0347510748956239 1102 18-19 0.04437280733704796 1102 20-21 -0.03202842918938131 1102 22-23 -0.03460651461456621 1102 24-25 -0.1983219990924212 1102 26-27 -0.16629748441247472 1102 28-29 -0.03802810397869649 1102 30-31 0.12861513140597225 1102 32-33 0.4344643461353215 1102 34-35 0.39147390455715225 1102 36-37 9.49223530433585E-4 1102 38-39 -0.08427647655227588 1102 40-41 0.4379565182775238 1102 42-43 0.5207057862246387 1102 44-45 0.5429378609239564 1102 46-47 0.4760985082406606 1102 48-49 0.6746712002590343 1102 50-51 0.6511848011458241 1102 52-53 0.7217776312313013 1102 54-55 0.6381820692061453 1102 56-57 0.35626389000621117 1102 58-59 0.31554976041580574 1102 60-61 0.4972271379592499 1102 62-63 0.736269190553628 1102 64-65 0.16777730732723128 1102 66-67 0.5766616961495572 1102 68-69 0.47696211114856624 1102 70-71 0.035154962672979906 1102 72-73 -0.07245994558716973 1102 74-75 -0.2786345224056497 1102 76-77 -0.17891618235680795 1102 78-79 -0.5305600716056844 1102 80-81 -0.4730704339013023 1102 82-83 -0.4576131015966105 1102 84-85 -0.30745137142198686 1102 86-87 -0.24059632539032094 1102 88-89 -0.5456299721981814 1102 90-91 -1.0101060426635051 1102 92-93 -0.7568321019785209 1102 94-95 -1.0381089000903145 1102 96-97 -0.9456092070617252 1102 98-99 -0.6869350608946263 1102 100-101 -0.34231676895387153 1103 1 0.29524979881733415 1103 2 0.07228912180851665 1103 3 0.3737334373814356 1103 4 0.41911098437284267 1103 5 0.3536251285281864 1103 6 0.49448775951369583 1103 7 0.18521296191082115 1103 8 0.13065005310247102 1103 9 0.35925416942348676 1103 10-11 0.1776764623486784 1103 12-13 0.08833526079131104 1103 14-15 -0.12787137203698506 1103 16-17 0.20705063318769135 1103 18-19 0.3066536265044917 1103 20-21 0.503868706401569 1103 22-23 0.40569598839707766 1103 24-25 0.26338828065593844 1103 26-27 0.5740066917352991 1103 28-29 0.8691630350923774 1103 30-31 0.5701975835542754 1103 32-33 0.4745108593196363 1103 34-35 0.36185939512066767 1103 36-37 0.4621742938021569 1103 38-39 0.8321754709781786 1103 40-41 0.7933808255991721 1103 42-43 0.7056709849932119 1103 44-45 0.9012901564667217 1103 46-47 0.8064257736669731 1103 48-49 0.48957215060035253 1103 50-51 -0.02593338919014343 1103 52-53 0.13129442951801096 1103 54-55 0.10602439285759857 1103 56-57 0.21049190499751091 1103 58-59 0.14939061170746726 1103 60-61 -0.27923350987906304 1103 62-63 -0.49247361496102826 1103 64-65 -0.3190383799970853 1103 66-67 -0.3320854595190248 1103 68-69 -0.46691253749284556 1103 70-71 -0.4983746852991793 1103 72-73 -0.5259049328713346 1103 74-75 -0.3841341208529805 1103 76-77 -0.18298190314384755 1103 78-79 -0.35933968611512057 1103 80-81 -0.21797573439654983 1103 82-83 -0.11524762174116887 1103 84-85 -0.042125444504574006 1103 86-87 -0.1737043430586347 1103 88-89 -0.29028430227447544 1103 90-91 -0.09485038746339569 1103 92-93 -0.29875252762435167 1103 94-95 -0.3056768294170418 1103 96-97 -0.11780837718620418 1103 98-99 0.21639113372457075 1103 100-101 0.41342208795952473 1104 1 -0.41899179349563553 1104 2 -0.6613587848489999 1104 3 -0.6804532475876819 1104 4 -0.5373577870753365 1104 5 -0.47856430180812026 1104 6 -0.5234429199646868 1104 7 -0.34239513692239854 1104 8 -0.47667664559347855 1104 9 -0.49433196647219546 1104 10-11 -0.5034148211104892 1104 12-13 -0.6416321379732679 1104 14-15 -0.8195837948235365 1104 16-17 -0.7603138143071604 1104 18-19 -0.484973003557279 1104 20-21 -0.2730101542710486 1104 22-23 -0.4654090218980471 1104 24-25 -0.5779723919590225 1104 26-27 -0.6876182224719756 1104 28-29 -0.42155419208675227 1104 30-31 -0.2933147705980872 1104 32-33 -0.29598502262957993 1104 34-35 -0.2081817853872252 1104 36-37 -0.017775946417479815 1104 38-39 -0.18620990994151754 1104 40-41 -0.21576467886205108 1104 42-43 -0.20587671576176803 1104 44-45 -0.6113042155305308 1104 46-47 -0.2722461549534785 1104 48-49 -0.2700537931196152 1104 50-51 -0.020820142793436247 1104 52-53 0.04344267934642687 1104 54-55 0.18793928647462366 1104 56-57 0.27839684665845965 1104 58-59 -0.19129229838175377 1104 60-61 0.021770265000832012 1104 62-63 0.1638493088550348 1104 64-65 0.1883741114236379 1104 66-67 0.061291342128193094 1104 68-69 0.46631498481325906 1104 70-71 0.1992231184070654 1104 72-73 -0.05499724584319665 1104 74-75 -0.0622312382174286 1104 76-77 -0.3408919923682845 1104 78-79 -0.4179618549552053 1104 80-81 -0.309593785185843 1104 82-83 -0.14633032592785256 1104 84-85 -0.08423251382509633 1104 86-87 -0.22116487840524002 1104 88-89 -0.07052623775834732 1104 90-91 0.09785723093056475 1104 92-93 0.3816953103989853 1104 94-95 0.35225041835234805 1104 96-97 0.0864469419627305 1104 98-99 0.40750300743767554 1104 100-101 0.578024009716561 1105 1 0.2559228374473612 1105 2 0.3302706696646176 1105 3 0.33141961818589394 1105 4 0.39098473984874715 1105 5 0.40559645469306105 1105 6 0.3311232301097391 1105 7 0.30805578681457746 1105 8 0.6144712398806504 1105 9 0.16711624296655714 1105 10-11 0.2902810023725664 1105 12-13 0.12134833659111877 1105 14-15 0.24653125639789408 1105 16-17 0.4700600583834955 1105 18-19 0.4900466969943338 1105 20-21 0.23975580317576117 1105 22-23 0.20783325110782158 1105 24-25 0.4933348490881784 1105 26-27 0.4187647562514272 1105 28-29 0.4072786596775373 1105 30-31 0.5883593384991883 1105 32-33 0.7474349931308666 1105 34-35 0.8296345179137106 1105 36-37 0.6921706431981391 1105 38-39 0.7661410225512526 1105 40-41 0.9031478506887751 1105 42-43 0.7912776490048898 1105 44-45 1.1344973780251948 1105 46-47 1.1597212734678521 1105 48-49 1.082778708412647 1105 50-51 0.8294031301248701 1105 52-53 0.5152052220309535 1105 54-55 0.7099338578781769 1105 56-57 0.9225173264763313 1105 58-59 1.0454645530323035 1105 60-61 0.9968600119581907 1105 62-63 1.318599330744533 1105 64-65 1.1995465131397758 1105 66-67 1.2105602327682448 1105 68-69 1.1923284687463678 1105 70-71 1.4239670112360656 1105 72-73 1.40202052843226 1105 74-75 1.4204672995638497 1105 76-77 1.5673997509116404 1105 78-79 1.419425414110549 1105 80-81 1.5201846930559952 1105 82-83 1.540807402817439 1105 84-85 1.3345837019178326 1105 86-87 1.371795457817509 1105 88-89 1.6763873424885674 1105 90-91 1.7870525728445799 1105 92-93 1.5963174975980046 1105 94-95 1.689278699588666 1105 96-97 1.4644961495627662 1105 98-99 1.703746768929669 1105 100-101 1.9494800993759611 1106 1 0.040582674589472845 1106 2 0.04076887472546886 1106 3 0.29827015666763756 1106 4 -0.043740765798744974 1106 5 0.04483995351102976 1106 6 0.05953580343956588 1106 7 0.15122188435419304 1106 8 0.161621226746945 1106 9 0.36949782076185045 1106 10-11 0.2582873065490858 1106 12-13 0.19028275564374297 1106 14-15 0.06765024772965234 1106 16-17 0.0013883133524146274 1106 18-19 0.304388698570385 1106 20-21 0.6440111223246987 1106 22-23 0.5665058717189666 1106 24-25 0.3816020761260859 1106 26-27 0.19748816050674378 1106 28-29 0.08270112706218669 1106 30-31 0.16200437833808223 1106 32-33 0.29975965822148964 1106 34-35 0.4428820444000081 1106 36-37 0.15243681953996457 1106 38-39 0.12230072835635042 1106 40-41 0.1331714913507156 1106 42-43 -0.15055756682559007 1106 44-45 0.20997238021414688 1106 46-47 -0.16160785708113679 1106 48-49 -0.3423942186515312 1106 50-51 -0.6420967385381182 1106 52-53 -0.30549349086634336 1106 54-55 -0.4588692241636778 1106 56-57 -0.3535180469585626 1106 58-59 -0.6274625111477121 1106 60-61 -0.5420595222332132 1106 62-63 -0.6957251592300722 1106 64-65 -0.8435407821933829 1106 66-67 -1.096155466382445 1106 68-69 -0.8209190577399283 1106 70-71 -0.5156704986142095 1106 72-73 -0.33584830967298274 1106 74-75 -0.445209961621682 1106 76-77 -0.21536007747466357 1106 78-79 -0.05838738687009837 1106 80-81 -0.14150867880286455 1106 82-83 -0.6420750067789207 1106 84-85 -0.39274315212296784 1106 86-87 -0.12116487840524215 1106 88-89 -0.21520708882217932 1106 90-91 -0.42554702438858527 1106 92-93 -0.35021958321803837 1106 94-95 0.007569567288516765 1106 96-97 0.3907022611116666 1106 98-99 0.2564391776504422 1106 100-101 0.022704860780390135 1107 1 0.09982075380904831 1107 2 0.18814083671352577 1107 3 0.23650916095659724 1107 4 0.007390341761599473 1107 5 0.31556571248840726 1107 6 0.08617780158942878 1107 7 0.2688066122996986 1107 8 0.19164393299536897 1107 9 -0.05304023322856466 1107 10-11 0.22960174604113348 1107 12-13 0.24437408522073412 1107 14-15 0.4690462615216049 1107 16-17 0.5077965212407349 1107 18-19 0.415094274215825 1107 20-21 0.4788862379583705 1107 22-23 0.48506612737450894 1107 24-25 0.3626886121617403 1107 26-27 0.26461455862296646 1107 28-29 0.20409545890979786 1107 30-31 0.28002641180877674 1107 32-33 0.5028880746709063 1107 34-35 0.5311504827147004 1107 36-37 0.5634030965562005 1107 38-39 0.7295919570166802 1107 40-41 0.5851856196830667 1107 42-43 0.6590211061203277 1107 44-45 0.5567472519658949 1107 46-47 0.47697594579499025 1107 48-49 0.8644765239268892 1107 50-51 0.6881109815863446 1107 52-53 0.5392378185273223 1107 54-55 0.2473287406836846 1107 56-57 -0.07813330219361347 1107 58-59 0.17411010679863637 1107 60-61 0.24807587428516342 1107 62-63 0.3905669944996397 1107 64-65 0.3291192127607836 1107 66-67 0.3502746067821363 1107 68-69 0.6864646778584209 1107 70-71 0.5841192583448311 1107 72-73 0.8200006517263958 1107 74-75 0.8984028547940959 1107 76-77 0.9342404607475174 1107 78-79 0.5428740713756355 1107 80-81 0.5196804558367809 1107 82-83 0.7427706748290213 1107 84-85 0.7363713041885269 1107 86-87 0.5691555319891783 1107 88-89 0.8207781100677387 1107 90-91 0.9276696941380322 1107 92-93 0.4364173690988373 1107 94-95 0.296006200035972 1107 96-97 0.2833773935647841 1107 98-99 0.1870337449702646 1107 100-101 0.044797199523980424 1108 1 0.07208633988537727 1108 2 -0.23284099475423758 1108 3 0.3251430334779357 1108 4 0.2272229388320426 1108 5 -0.09066871804269994 1108 6 0.26173498098292924 1108 7 -0.07654496706722114 1108 8 0.034587434506647696 1108 9 0.13497252128035342 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-1.1663749140944617 2316 96-97 -1.5787458771862042 2316 98-99 -1.740577112243173 2316 100-101 -1.4026691418791835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2203.0 3 357.0 4 307.0 5 294.0 6 308.0 7 366.0 8 414.0 9 442.0 10 524.0 11 675.0 12 984.0 13 1468.0 14 1275.0 15 1588.0 16 1953.0 17 2396.0 18 2179.0 19 1832.0 20 2043.0 21 2144.0 22 2353.0 23 2680.0 24 3122.0 25 3495.0 26 3945.0 27 4462.0 28 5117.0 29 6118.0 30 7261.0 31 8678.0 32 11011.0 33 15305.0 34 29230.0 35 79050.0 36 16695.0 37 14341.0 38 9003.0 39 713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.903451644415505 23.522468251383913 20.404591338326277 7.169488765874308 2 12.101540823912222 31.580593662026185 41.794797840490396 14.523067673571195 3 19.10971898905938 41.74009839648665 32.713784852141195 6.436397762312765 4 7.677837952034842 27.120668570755313 37.11347745361323 28.088016023596612 5 5.161919466905043 48.21949534760879 31.585876234204218 15.032708951281947 6 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5.941985712747829 28-29 5.706840205630673 44.1484424349889 44.58655519875533 5.5581621606250975 30-31 5.638499893371788 45.561727580148876 43.6958354066597 5.103937119819646 32-33 4.963464514478125 45.74389010515883 43.17665790681522 6.115987473547821 34-35 5.031117587440624 45.1465518294048 43.39143035073938 6.430900232415206 36-37 6.121579617834395 44.86542675159236 43.60152866242038 5.411464968152866 38-39 6.8876844078572015 44.27907734941723 43.40940581954519 5.423832423180373 40-41 5.302897564513697 45.03530782802867 44.319575572606176 5.342219034851454 42-43 5.2913352449311075 45.72386990537795 43.36820267012303 5.616592179567913 44-45 5.46500655254571 45.637716575393 42.51226436821434 6.385012503846946 46-47 5.075070362542874 45.50912725990629 42.741499939878494 6.67430243767234 48-49 5.624814000986559 44.95305690652556 42.96628169117437 6.45584740131351 50-51 5.651501285126974 45.246456983406475 43.28849826645442 5.813543465012138 52-53 4.770756643715834 45.30262079761279 43.28902151181988 6.637601046851496 54-55 4.381330887598084 44.832365229797205 43.82696423112198 6.959339651482726 56-57 5.571970005705436 45.07172548699975 42.24610807726791 7.110196430026897 58-59 5.158928240349294 44.281904327257095 43.369144226283005 7.190023206110603 60-61 5.353833244398098 43.70012668179245 43.80644162657792 7.139598447231535 62-63 5.857981050825281 43.81767380436577 43.28392580923248 7.040419335576474 64-65 5.232840189647652 44.24004370212437 44.189900406446064 6.337215701781922 66-67 5.269756234787607 43.96396726965839 43.729538440212494 7.0367380553415115 68-69 4.964332241248198 44.41640044118337 43.57093490506607 7.04833241250237 70-71 5.15445211902762 44.09489753480065 42.76933301801398 7.981317328157747 72-73 5.355737751703162 43.137202940325096 43.10336392434738 8.403695383624363 74-75 5.609633288417671 43.1958886879802 43.311881178027065 7.88259684557506 76-77 5.773773251512736 42.368449477955515 43.94304079956468 7.914736470967071 78-79 5.576736158515587 42.2546576383639 44.65403166154868 7.51457454157183 80-81 5.434100375777436 42.9356287547176 43.717954988221294 7.912315881283676 82-83 4.8693054213896465 42.65069718720322 43.447948382468624 9.032049008938506 84-85 5.461719055693231 41.83799073953306 43.20280911699491 9.497481087778791 86-87 6.041831828955386 41.35514256774796 43.62730012900491 8.975725474291748 88-89 6.425492453964599 40.33567374224251 43.932090776614935 9.306743027177957 90-91 5.884463215225531 40.32535869563002 44.50006828415176 9.290109804992692 92-93 5.665723534543913 40.909850702456765 44.089863911742405 9.33456185125692 94-95 5.450712810612346 40.90409624013553 43.92332265765316 9.721868291598968 96-97 5.681280780144157 39.9927839926943 43.40632236391507 10.919612863246469 98-99 6.348957197285513 39.47504143283192 43.144981276233146 11.031020093649412 100-101 6.766983187505478 38.66792215177103 44.46312725849745 10.101967402226041 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 36379.0 1 21344.0 2 6800.5 3 6578.0 4 4405.0 5 4221.5 6 10114.5 7 18831.5 8 22696.0 9 19335.5 10 12171.0 11 7398.5 12 6610.0 13 5895.0 14 4323.0 15 2692.5 16 1658.5 17 1243.0 18 1065.0 19 925.0 20 883.0 21 864.0 22 824.5 23 826.5 24 829.0 25 852.0 26 921.5 27 980.0 28 1016.5 29 1084.0 30 1240.5 31 1308.5 32 1347.0 33 1462.0 34 1560.5 35 1456.5 36 1237.0 37 1104.5 38 972.5 39 871.0 40 794.5 41 710.5 42 642.5 43 625.0 44 782.5 45 876.0 46 1450.5 47 3323.0 48 9945.0 49 10828.0 50 6872.5 51 5098.5 52 1735.5 53 582.5 54 411.5 55 349.5 56 331.0 57 303.0 58 272.5 59 261.5 60 236.0 61 199.5 62 181.0 63 191.0 64 173.5 65 144.0 66 133.0 67 109.5 68 96.0 69 84.5 70 77.5 71 69.0 72 54.5 73 44.5 74 38.0 75 36.5 76 31.0 77 25.0 78 18.0 79 14.5 80 13.0 81 11.0 82 8.5 83 3.5 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26427855202958617 2 0.06657708530392034 3 0.07469624204830086 4 0.07875582042049113 5 0.08971668202540484 6 0.04221961507077875 7 0.04506131993131193 8 0.04506131993131193 9 0.04709110911740706 10-11 0.04627919344296901 12-13 0.04404642533826437 14-15 0.04323450966382632 16-17 0.04404642533826437 18-19 0.05115068748959733 20-21 0.051759624245425864 22-23 0.06393835936199667 24-25 0.0722604950249867 26-27 0.06799793773418693 28-29 0.06556219071087277 30-31 0.060284738827025425 32-33 0.053586434512911486 34-35 0.26387259419236714 36-37 0.4134680572075784 38-39 0.38606590319529416 40-41 0.37266929456706627 42-43 0.40047740641656954 44-45 0.4085965631609501 46-47 0.4037250691143218 48-49 0.41976040368447337 50-51 0.41590380423089257 52-53 0.4157008253122831 54-55 0.40839358424234057 56-57 0.38606590319529416 58-59 0.37409014699733284 60-61 0.3385688362406681 62-63 0.4021012377654457 64-65 0.41976040368447337 66-67 0.4278795604288539 68-69 0.4390434009523771 70-71 0.43721659068489144 72-73 0.42706764475441583 74-75 0.4288944550219014 76-77 0.4049429426259789 78-79 0.38748675562556073 80-81 0.39499697561411273 82-83 0.4010863431723981 84-85 0.4006803853351791 86-87 0.40250719560266474 88-89 0.407378689649293 90-91 0.4191514669286448 92-93 0.42584977124275875 94-95 0.4341719069057488 96-97 0.4234140242194446 98-99 0.4280825393474633 100-101 0.40900252099816914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 246331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.298115137761792 #Duplication Level Percentage of deduplicated Percentage of total 1 80.49835473941195 12.314730992039166 2 8.703959239995754 2.6630834121568134 3 3.16579980893748 1.4529230994068956 4 1.7434454941089057 1.0668571962116016 5 1.0083855217068252 0.7713198907161503 6 0.6952552807557584 0.6381657201083095 7 0.5519583908289991 0.5910746109909025 8 0.36354951703640803 0.444929789592053 9 0.29720836429253794 0.4092054999167786 >10 2.1680288716696743 6.655272783368719 >50 0.29986201040229277 3.2521282339616207 >100 0.38212503980469165 12.928133284077115 >500 0.06103386052436047 6.852162334419948 >1k 0.047765629975586456 14.509745017882441 >5k 0.002653646109754803 3.9024726891865007 >10k+ 0.010614584439019211 31.547795445964983 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27777 11.276290844432896 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22753 9.236758670244509 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16187 6.57123951106438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10995 4.4635064202231955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9613 3.9024726891865007 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4832 1.9615882694423357 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4524 1.8365532555788755 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3278 1.3307297904039685 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3224 1.3088080671941413 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2083 0.845610174927232 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1843 0.7481802939946657 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1745 0.7083964259472011 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1680 0.6820091665279644 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1624 0.6592755276436989 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1552 0.6300465633639291 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1499 0.6085307979913206 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.553726489966752 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1157 0.46969321766241356 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1113 0.4518310728247764 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1087 0.44127616905708167 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1085 0.44046425338264367 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1044 0.4238199820566636 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.4092054999167786 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 986 0.40027442749796005 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 949 0.3852539875208561 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 938 0.3807884513114468 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 925 0.37551099942759947 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 875 0.35521310756664815 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 860 0.3491237400083627 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 810 0.3288258481474114 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 754 0.3060922092631459 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 742 0.3012207152165176 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 738 0.2995968838676415 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 724 0.29391347414657515 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 717 0.29107176928604195 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 698 0.28335857037888046 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.2756453714717189 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 676 0.2744274979600619 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 665 0.26996196175065257 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.26021897365739594 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 634 0.25737726879686273 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 631 0.2561593952852057 No Hit GAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 621 0.2520998169130154 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 582 0.23626746126147335 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 526 0.21353382237720792 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 508 0.2062265813072654 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 493 0.20013721374898003 No Hit GCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 490 0.19891934023732297 No Hit GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 469 0.1903942256557234 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 450 0.1826810267485619 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 442 0.17943336405080967 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 437 0.17740357486471456 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.1749678278414004 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 429 0.17415591216696233 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 420 0.1705022916319911 No Hit GTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 416 0.168878460283115 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 409 0.1660367554225818 No Hit GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 409 0.1660367554225818 No Hit GTACATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 395 0.16035334570151544 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 391 0.15872951435263932 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 376 0.15264014679435392 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 367 0.14898652625938272 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 366 0.1485805684221637 No Hit GTACATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 361 0.14655077923606852 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 348 0.1412733273522212 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 347 0.14086736951500217 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 346 0.14046141167778314 No Hit ACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 344 0.1396494960033451 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 343 0.13924353816612606 No Hit GTACATGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 330 0.13396608628227874 No Hit GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 330 0.13396608628227874 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 329 0.1335601284450597 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 316 0.12828267656121237 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 311 0.12625288737511722 No Hit ACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 305 0.12381714035180305 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 304 0.12341118251458404 No Hit TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 301 0.12219330900292695 No Hit GTACATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 301 0.12219330900292695 No Hit GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 301 0.12219330900292695 No Hit GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 299 0.1213813933284889 No Hit GTACATGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 298 0.12097543549126988 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 290 0.11772777279351765 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 289 0.11732181495629863 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 285 0.11569798360742253 No Hit GTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.1152920257702035 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 276 0.1120443630724513 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 268 0.10879670037469907 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 263 0.10676691118860396 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 262 0.10636095335138493 No Hit GAGTACATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 260 0.10554903767694687 No Hit GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 259 0.10514307983972786 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 255 0.10351924849085173 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 251 0.10189541714197563 No Hit GTACATGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250 0.1014894593047566 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250 0.1014894593047566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.0297891860951322E-4 0.0 16-17 0.0 0.0 0.0 8.119156744380529E-4 0.0 18-19 0.0 0.0 0.0 0.0012178735116570793 0.0 20-21 0.0 0.0 0.0 0.0024357470233141586 0.0 22-23 0.0 0.0 0.0 0.005277451883847343 0.0 24-25 0.0 0.0 0.0 0.009540009174647122 0.0 26-27 0.0 0.0 0.0 0.014005545384056413 0.0 28-29 0.0 0.0 0.0 0.021312786453998887 0.0 30-31 0.0 0.0 0.0 0.037145142105540915 0.0 32-33 0.0 0.0 0.0 0.062314528013120554 0.0 34-35 0.0 0.0 0.0 0.09966264903727098 0.0 36-37 0.0 0.0 0.0 0.161368240294563 0.0 38-39 0.0 0.0 0.0 0.2287572412729214 0.0 40-41 0.0 0.0 0.0 0.3115726400656028 0.0 42-43 0.0 0.0 0.0 0.3704365264623616 0.0 44-45 0.0 0.0 0.0 0.430315307452168 0.0 46-47 0.0 0.0 0.0 0.5015609078841071 0.0 48-49 0.0 0.0 0.0 0.5764601288510176 0.0 50-51 0.0 0.0 0.0 0.6594785065623084 0.0 52-53 0.0 0.0 0.0 0.7345807064478282 0.0 54-55 0.0 0.0 0.0 0.8058263068797674 0.0 56-57 0.0 0.0 0.0 0.8878297899980108 0.0 58-59 0.0 0.0 0.0 0.9954086168610528 0.0 60-61 0.0 0.0 0.0 1.1525142998648161 0.0 62-63 0.0 0.0 0.0 1.281811871019076 0.0 64-65 0.0 0.0 0.0 1.3863460141029753 0.0 66-67 0.0 0.0 0.0 1.5081333652686832 0.0 68-69 0.0 0.0 0.0 1.6455500931673237 0.0 70-71 0.0 0.0 0.0 1.820111963171505 0.0 72-73 0.0 0.0 0.0 2.000560221815362 0.0 74-75 0.0 0.0 0.0 2.187909763691943 0.0 76-77 0.0 0.0 0.0 2.3594269499169815 0.0 78-79 0.0 0.0 0.0 2.535612651270039 0.0 80-81 0.0 0.0 0.0 2.7280366661118576 0.0 82-83 0.0 0.0 0.0 2.953140286849808 0.0 84-85 0.0 0.0 0.0 3.226958848054041 0.0 86-87 0.0 0.0 0.0 3.528991478944997 0.0 88-89 0.0 0.0 0.0 3.7800764012649646 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATGCT 15 6.683771E-4 94.74121 4 AGCACAT 30 1.31818E-4 63.147987 7 GGATGGG 25 0.0050751353 56.833187 8 ATGGGTT 115 0.0 53.54938 5 GCATCAA 55 8.319348E-7 51.67703 2 GGCATCA 60 1.4945927E-6 47.467026 1 CTTGGTA 25 2.8739738E-5 47.36099 16-17 GCACTTT 20 5.463535E-4 47.36099 14-15 CATCAAC 75 1.386361E-7 44.21257 3 TATGGGA 65 2.6299476E-6 43.726715 4 CGCAGAA 260 0.0 40.07468 9 TTGGTAT 30 8.428137E-5 39.46749 16-17 GGAGTGA 60 8.415852E-5 39.46749 8 TGGGAGT 85 3.709465E-7 39.003166 6 TGGGTGG 160 0.0 38.480804 6 GGGTGAG 75 7.040342E-6 37.888794 7 GGTGAGA 75 7.040342E-6 37.888794 8 GAGCACT 25 0.0016383381 37.88879 12-13 TGGGTTG 125 1.382432E-10 37.888786 6 TGGGAAT 130 2.1100277E-10 36.43153 6 >>END_MODULE