##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939019_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 246331 Sequences flagged as poor quality 0 Sequence length 101 %GC 16 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.515887159959565 31.0 31.0 34.0 30.0 34.0 2 31.607012515680122 31.0 31.0 34.0 30.0 34.0 3 31.542712853843 31.0 31.0 34.0 28.0 34.0 4 35.32939824869789 37.0 35.0 37.0 33.0 37.0 5 35.343286066309155 37.0 35.0 37.0 33.0 37.0 6 35.547125615533574 37.0 35.0 37.0 33.0 37.0 7 35.44347646053481 37.0 35.0 37.0 32.0 37.0 8 35.552585748444166 37.0 35.0 37.0 33.0 37.0 9 36.89271346277975 39.0 37.0 39.0 32.0 39.0 10-11 36.83481778582477 39.0 37.0 39.0 32.0 39.0 12-13 36.63981390892742 39.0 36.0 39.0 32.0 39.0 14-15 37.32479062724545 39.0 36.0 41.0 32.0 41.0 16-17 37.19170952904831 38.5 36.0 40.5 32.0 41.0 18-19 36.93384511084679 38.0 35.0 40.0 32.0 41.0 20-21 36.8899184430705 38.5 35.0 41.0 32.0 41.0 22-23 36.55459929931678 38.0 35.0 41.0 31.5 41.0 24-25 36.2314162651067 38.0 35.0 40.5 31.0 41.0 26-27 35.66930471601219 36.5 35.0 40.0 30.5 41.0 28-29 35.13209056107432 35.0 35.0 39.5 30.0 41.0 30-31 34.37287024369649 35.0 34.0 38.0 29.0 40.0 32-33 33.23096362211821 35.0 33.0 36.0 24.5 40.0 34-35 32.75026488748878 35.0 33.0 35.0 23.0 39.0 36-37 32.322957727610415 35.0 33.0 35.0 21.0 39.0 38-39 31.97054775890976 35.0 33.0 35.0 21.0 38.0 40-41 31.61364992631865 35.0 33.0 35.0 20.5 38.0 42-43 31.31934673264835 35.0 33.0 35.0 20.0 38.0 44-45 30.99749524014436 35.0 31.0 35.0 19.0 38.0 46-47 30.49457843308394 35.0 31.0 35.0 20.0 38.0 48-49 30.083499437748394 35.0 29.0 35.0 15.5 37.5 50-51 29.99542485517454 35.0 28.0 35.0 15.0 37.0 52-53 29.817199215689456 35.0 28.0 35.0 15.0 37.0 54-55 29.513059663623338 34.0 27.0 35.0 12.0 36.0 56-57 29.704947814120025 34.5 28.5 35.0 11.5 36.0 58-59 29.619668251255426 35.0 29.0 35.0 6.5 36.0 60-61 29.2773849008042 34.0 28.0 35.0 2.0 35.0 62-63 28.754328525439348 33.0 27.0 35.0 2.0 35.0 64-65 28.351835538360987 33.0 27.0 35.0 2.0 35.0 66-67 27.886977278539852 33.0 26.0 35.0 2.0 35.0 68-69 27.46983936248381 33.0 26.0 35.0 2.0 35.0 70-71 26.98955267505917 33.0 25.0 35.0 2.0 35.0 72-73 26.49821581530542 33.0 24.0 35.0 2.0 35.0 74-75 25.93391818325749 33.0 22.5 35.0 2.0 35.0 76-77 24.03964584238281 30.0 20.0 33.0 2.0 35.0 78-79 25.275188668904846 32.0 20.0 35.0 2.0 35.0 80-81 25.37554550584376 33.0 20.0 35.0 2.0 35.0 82-83 24.938257872537356 33.0 17.5 35.0 2.0 35.0 84-85 24.423738790489217 33.0 8.5 35.0 2.0 35.0 86-87 23.52252863017647 31.0 2.0 35.0 2.0 35.0 88-89 22.578270700805014 31.0 2.0 35.0 2.0 35.0 90-91 21.805054986989052 29.0 2.0 34.0 2.0 35.0 92-93 21.27002488521542 29.0 2.0 34.0 2.0 35.0 94-95 20.65547576228733 29.0 2.0 33.5 2.0 35.0 96-97 19.73414836135119 28.0 2.0 33.0 2.0 35.0 98-99 18.600486743446826 25.5 2.0 33.0 2.0 35.0 100-101 13.131167006994655 2.0 2.0 25.5 2.0 30.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34511126844484963 1101 2 0.39302636385090395 1101 3 0.19360824179299385 1101 4 0.04688463351129002 1101 5 0.15387448033516904 1101 6 0.20732016027856304 1101 7 0.22792191417054397 1101 8 0.308549306662357 1101 9 0.34830961948308214 1101 10-11 0.45177982130610417 1101 12-13 0.1857693746502136 1101 14-15 0.7541457395135183 1101 16-17 0.15208764251205054 1101 18-19 0.28971550248725464 1101 20-21 0.6169338223886527 1101 22-23 0.35417284455470366 1101 24-25 0.6268961638750383 1101 26-27 0.6482961976534725 1101 28-29 0.798800767424197 1101 30-31 0.7764812252615343 1101 32-33 0.27529054679869347 1101 34-35 0.8106317517083639 1101 36-37 0.8166722089927063 1101 38-39 0.7319686554635574 1101 40-41 1.0090988632553142 1101 42-43 1.1429654588311742 1101 44-45 1.1916384396044073 1101 46-47 0.65841116047417 1101 48-49 0.9391318424732269 1101 50-51 0.4701596387590108 1101 52-53 0.9548096303478317 1101 54-55 0.9125664857514799 1101 56-57 0.6904770209707607 1101 58-59 1.2610766326103544 1101 60-61 1.4966115052266673 1101 62-63 1.4383870164820642 1101 64-65 1.0076920167983872 1101 66-67 0.524208857684016 1101 68-69 0.7824446140113253 1101 70-71 0.6805808119449956 1101 72-73 0.29565993232471044 1101 74-75 0.6512803781271614 1101 76-77 0.18083360189885767 1101 78-79 0.8540458470639258 1101 80-81 0.9612218153336265 1101 82-83 0.7990211835677279 1101 84-85 0.825163835536376 1101 86-87 0.7202927435685034 1101 88-89 0.8103246666645703 1101 90-91 0.7048492041047218 1101 92-93 0.6129896204827467 1101 94-95 0.7690490102594012 1101 96-97 0.4766345727226522 1101 98-99 0.603617521406516 1101 100-101 0.8288671319304459 1102 1 -0.27584477514048444 1102 2 -0.13792529421339594 1102 3 -0.4417356842603759 1102 4 -0.14029277768190695 1102 5 -0.3168268656824438 1102 6 -0.11690478321568776 1102 7 -0.05624298142031847 1102 8 -0.02618401992402397 1102 9 -0.08587383023523643 1102 10-11 0.20063283888192274 1102 12-13 -0.09751841673392647 1102 14-15 -0.0882285141045287 1102 16-17 -0.5067609895427339 1102 18-19 -0.1495450311969222 1102 20-21 0.3075943493258677 1102 22-23 0.16788097126252666 1102 24-25 -0.2740585303192091 1102 26-27 -0.41520108669697464 1102 28-29 -0.17502947917702016 1102 30-31 0.17760459334169099 1102 32-33 0.09339262823444727 1102 34-35 0.282598151870296 1102 36-37 0.313863788792311 1102 38-39 0.4171894495802029 1102 40-41 0.8347322336969079 1102 42-43 0.4742409707564157 1102 44-45 0.3277076633671854 1102 46-47 0.2677624407089638 1102 48-49 0.28462656298059485 1102 50-51 0.5376544942054942 1102 52-53 0.36401247756302624 1102 54-55 -0.050881883172042564 1102 56-57 0.15225551842378593 1102 58-59 0.10485087948145377 1102 60-61 -0.2572005245948752 1102 62-63 -0.6078633630341805 1102 64-65 -0.7701307406823048 1102 66-67 -1.3109039851456572 1102 68-69 -0.9308013462491047 1102 70-71 -0.6023527642787379 1102 72-73 -0.37380672084804445 1102 74-75 -0.4090964393339611 1102 76-77 -0.9298586357289089 1102 78-79 -0.5132668405857714 1102 80-81 -0.4007776449099403 1102 82-83 -0.8579973368249441 1102 84-85 -0.18480479112084325 1102 86-87 -0.013868778544640747 1102 88-89 0.06530897999317986 1102 90-91 0.028880172630177725 1102 92-93 -0.11746107614040113 1102 94-95 -0.3842165161717972 1102 96-97 -0.05729417942794157 1102 98-99 0.1209234621007802 1102 100-101 -0.17929668414755007 1103 1 0.0034183757094687905 1103 2 0.07038015351783145 1103 3 -0.05458871598303716 1103 4 -0.14971253407328788 1103 5 0.020711616448465975 1103 6 0.1728656625077747 1103 7 0.03676665584377048 1103 8 -0.0976269325193897 1103 9 0.07191963784132582 1103 10-11 0.07641921286131037 1103 12-13 -0.20209779031176112 1103 14-15 -0.33535071996720234 1103 16-17 0.09488203013334129 1103 18-19 -0.05284612877421324 1103 20-21 -0.2600003501788848 1103 22-23 0.022150814656981765 1103 24-25 -0.25716822112365634 1103 26-27 -0.14274759988128238 1103 28-29 -0.12225876575177352 1103 30-31 -0.2608909226534948 1103 32-33 0.16414118933737853 1103 34-35 0.3001577155163915 1103 36-37 -0.49513935670360354 1103 38-39 -0.9896252338624372 1103 40-41 -0.42917152751310894 1103 42-43 -0.786207764352671 1103 44-45 -0.9527852425356365 1103 46-47 -1.2340947403912885 1103 48-49 -1.5733911053368992 1103 50-51 -1.6665601356967983 1103 52-53 -0.9361063151019415 1103 54-55 -0.8766414869734085 1103 56-57 -1.4253110723940452 1103 58-59 -1.4294065827056706 1103 60-61 -1.8442454315685062 1103 62-63 -1.4641995295446897 1103 64-65 -1.6137510726325104 1103 66-67 -1.5854488921192917 1103 68-69 -1.6931323595003427 1103 70-71 -1.1755692011680416 1103 72-73 -1.6054022234581353 1103 74-75 -1.8224279879887604 1103 76-77 -1.802348931539381 1103 78-79 -1.2838213178980453 1103 80-81 -1.1020977493136321 1103 82-83 -1.3802667786667335 1103 84-85 -0.9971709402307347 1103 86-87 -0.8661057615455796 1103 88-89 -1.4529031067421982 1103 90-91 -2.093685414305977 1103 92-93 -1.8639642216363193 1103 94-95 -1.7907300351117001 1103 96-97 -1.6577576381349388 1103 98-99 -1.1434320458633493 1103 100-101 -0.45062080407794625 1104 1 0.11774576074721921 1104 2 -0.08318882383288795 1104 3 0.0767599456504584 1104 4 -0.016270529955235702 1104 5 -0.2221504288500995 1104 6 0.0775156281906888 1104 7 0.19701030718213275 1104 8 0.18737649918959676 1104 9 0.29297317250158983 1104 10-11 0.020894161385669463 1104 12-13 -0.029971846591784868 1104 14-15 -0.0029897041813384817 1104 16-17 0.19369809053142006 1104 18-19 0.08334982180917194 1104 20-21 -0.20907378875129012 1104 22-23 -0.006984394677267858 1104 24-25 0.04865710488870434 1104 26-27 0.17907806930197268 1104 28-29 -0.08811326129055175 1104 30-31 -0.14489915875507364 1104 32-33 -0.08701701244711302 1104 34-35 -0.3849572467348068 1104 36-37 -0.3394512990508929 1104 38-39 -0.20843957840601846 1104 40-41 -0.4417487409654086 1104 42-43 -0.16068442873153188 1104 44-45 -0.17540878405931792 1104 46-47 -0.1974269298216278 1104 48-49 -0.2754158136416329 1104 50-51 -0.2618157980166309 1104 52-53 -0.24961866925431409 1104 54-55 0.006097016801472677 1104 56-57 -0.28001251371182434 1104 58-59 0.27839197597655385 1104 60-61 0.14950027879525507 1104 62-63 -0.01106809509032658 1104 64-65 -0.1395489449729368 1104 66-67 -0.5084585009044638 1104 68-69 -0.9433554205847656 1104 70-71 -0.5564888991776478 1104 72-73 -0.11689844857824383 1104 74-75 0.3848472350723249 1104 76-77 0.11832711513186212 1104 78-79 0.15213441305597186 1104 80-81 -0.1329573924159 1104 82-83 -0.028430814356504186 1104 84-85 -0.4241355936281259 1104 86-87 -0.3952495501523039 1104 88-89 -0.2309830655616878 1104 90-91 -0.33046475541785014 1104 92-93 0.2781600062291609 1104 94-95 0.675596320413355 1104 96-97 0.824766383827999 1104 98-99 0.6056413927090603 1104 100-101 0.5074694498685179 1105 1 0.3023530630850182 1105 2 0.06802385456655102 1105 3 0.12911963394395087 1105 4 -0.034867850680214474 1105 5 0.04908676579135118 1105 6 0.17570317744206676 1105 7 0.11726247424281411 1105 8 0.10936231479243474 1105 9 0.15825642265481576 1105 10-11 0.23525367456315394 1105 12-13 0.06452075475660735 1105 14-15 0.1010292083482085 1105 16-17 0.028756316606198595 1105 18-19 0.1670114976694279 1105 20-21 0.4398536256607599 1105 22-23 0.25031356522116965 1105 24-25 0.36196592198002264 1105 26-27 0.49610810169843234 1105 28-29 0.8970894354960635 1105 30-31 0.5517999043742563 1105 32-33 0.8024410034884468 1105 34-35 0.8091763667841505 1105 36-37 0.3722026717047342 1105 38-39 0.8129683543494259 1105 40-41 0.6645992145551283 1105 42-43 0.5458561544047953 1105 44-45 0.714171813086292 1105 46-47 0.39302924739577705 1105 48-49 0.8204426926187658 1105 50-51 0.7247265362798352 1105 52-53 0.7620965921063387 1105 54-55 1.4149753987294993 1105 56-57 0.7957164066979487 1105 58-59 1.0763431186085448 1105 60-61 1.0060715072340969 1105 62-63 1.4795894655435902 1105 64-65 1.1884608615238683 1105 66-67 1.6313803923623276 1105 68-69 1.118513067288454 1105 70-71 1.4109833011550705 1105 72-73 1.5269155782145027 1105 74-75 1.3466281649383625 1105 76-77 1.4565286736643905 1105 78-79 1.4354721242896638 1105 80-81 1.4655628772627658 1105 82-83 1.6660792985933242 1105 84-85 1.9986557556407618 1105 86-87 1.9245735826731938 1105 88-89 2.027076648999209 1105 90-91 2.137173805128512 1105 92-93 2.5581617573893034 1105 94-95 2.640398001527089 1105 96-97 1.86961359510547 1105 98-99 2.360417682000719 1105 100-101 1.4799762356140764 1106 1 0.07093725010891916 1106 2 0.0019175591458342467 1106 3 0.008674839267477807 1106 4 0.10287840621497679 1106 5 0.003381486043522841 1106 6 -0.003335435639101547 1106 7 0.02679754122468836 1106 8 -0.3998575433635878 1106 9 -0.7921332104771324 1106 10-11 -0.24293562584836792 1106 12-13 -0.5661420593577375 1106 14-15 -0.21788332120262055 1106 16-17 -0.008429569043038043 1106 18-19 0.05994556649002192 1106 20-21 -0.22183974619809987 1106 22-23 -0.1048567351027927 1106 24-25 -0.04070459723895681 1106 26-27 -0.5039006541022815 1106 28-29 -0.747687729375663 1106 30-31 -0.6278778821593249 1106 32-33 -0.16148509755349494 1106 34-35 -0.1487870339688513 1106 36-37 0.007357211587404322 1106 38-39 -0.42120553585283105 1106 40-41 -0.23323810266753497 1106 42-43 -0.7287695351145125 1106 44-45 -0.43498325214442346 1106 46-47 -0.6250865042897118 1106 48-49 -0.2817987923650378 1106 50-51 -0.6447945214208879 1106 52-53 -1.51983143521176 1106 54-55 -0.6790093661772509 1106 56-57 -0.9821401732862896 1106 58-59 -1.2641612155128108 1106 60-61 -0.8696486573749596 1106 62-63 -1.3621319248775627 1106 64-65 -1.8757191577388923 1106 66-67 -1.8595223306916964 1106 68-69 -1.7646320163294433 1106 70-71 -1.6203186864116894 1106 72-73 -1.4530686613442043 1106 74-75 -1.1896208500340606 1106 76-77 -1.9157154380596246 1106 78-79 -1.8372272890716879 1106 80-81 -1.1308297328414305 1106 82-83 -1.0816223037182056 1106 84-85 -1.0709441042664274 1106 86-87 -0.36546231610975255 1106 88-89 -0.39694051237020034 1106 90-91 0.17591822330555473 1106 92-93 0.4568834104844761 1106 94-95 0.44580908637080086 1106 96-97 0.058808937019485086 1106 98-99 0.07585415866650536 1106 100-101 0.5691715775280937 1107 1 -0.1493133595958973 1107 2 -0.14079541705465104 1107 3 0.021928560569303812 1107 4 0.09176075420926111 1107 5 -0.0025894163196085174 1107 6 0.14642488729420933 1107 7 0.010835889555359302 1107 8 0.067201576979528 1107 9 -0.22839593018110804 1107 10-11 -0.3014336495637835 1107 12-13 -0.13406738102959537 1107 14-15 -0.09045080921876547 1107 16-17 0.1583940790942009 1107 18-19 -0.030812149429578994 1107 20-21 0.002590495160916362 1107 22-23 -0.195017360786089 1107 24-25 0.06516538846451425 1107 26-27 0.034161746032275175 1107 28-29 0.04295225044100448 1107 30-31 -0.027625590510176323 1107 32-33 0.4720192334531461 1107 34-35 -0.08399853846805883 1107 36-37 0.272591422335033 1107 38-39 0.04780463991034978 1107 40-41 -0.03306990823476852 1107 42-43 -0.21779484837664143 1107 44-45 -0.14674004299464727 1107 46-47 0.16554506580531836 1107 48-49 0.5443890808163623 1107 50-51 0.1493186734323615 1107 52-53 0.11265531947666219 1107 54-55 0.09425612873486955 1107 56-57 0.26753605243063916 1107 58-59 0.22910259745497896 1107 60-61 0.5920114216764247 1107 62-63 0.7024379178606388 1107 64-65 0.7027014965374505 1107 66-67 0.690481874169123 1107 68-69 0.8771054321610094 1107 70-71 0.363828988300277 1107 72-73 1.0145455006270332 1107 74-75 0.7958303315318318 1107 76-77 0.6486105227510706 1107 78-79 1.102390909565937 1107 80-81 0.12145350658334664 1107 82-83 -0.33584818884141043 1107 84-85 0.010789980335371041 1107 86-87 0.44758957187275783 1107 88-89 0.8291716733164556 1107 90-91 0.64900711406327 1107 92-93 0.43019936372642675 1107 94-95 0.2497280166542062 1107 96-97 0.16773165167763437 1107 98-99 0.11176367006168064 1107 100-101 -0.443249581331548 1108 1 -0.12564776687659673 1108 2 0.1369971228862248 1108 3 0.10626110781745268 1108 4 -0.05385322260676162 1108 5 0.2131079298125158 1108 6 0.14504647031477447 1108 7 0.34819929341492184 1108 8 0.13177127837786884 1108 9 0.2650275261579438 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-0.0864370736188107 2316 98-99 -0.4680288200568974 2316 100-101 0.06494119188979575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 52.0 5 279.0 6 584.0 7 710.0 8 731.0 9 1043.0 10 1280.0 11 1450.0 12 1541.0 13 1768.0 14 2108.0 15 2643.0 16 2982.0 17 3421.0 18 3707.0 19 3495.0 20 3860.0 21 4352.0 22 5018.0 23 5510.0 24 6132.0 25 6812.0 26 7473.0 27 8452.0 28 9412.0 29 10596.0 30 11798.0 31 13476.0 32 16177.0 33 21692.0 34 39617.0 35 42509.0 36 3614.0 37 1518.0 38 470.0 39 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.423058595452304 24.974850426219092 22.596332031393185 6.005758946935417 2 13.120963256756154 33.520750534849455 39.88210984407159 13.4761763643228 3 20.631183245308144 39.148949990054035 34.61155924345697 5.60830752118085 4 8.603464444182828 27.966435406018732 35.7855893086944 27.644510841104044 5 4.793550141882264 49.70507163126038 31.872155757902988 13.629222468954374 6 15.140603496920809 40.2024105776374 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41.23841497822036 47.52690485566169 4.924065586548181 30-31 5.903641847757692 42.941814063191394 46.24164234302625 4.912901746024658 32-33 5.409794138780747 42.96048812370347 46.57838436899944 5.051333368516346 34-35 5.550525576428841 42.18618682019156 46.515848494321986 5.747439109057616 36-37 6.461737734006549 42.04798999514781 46.31954214807912 5.17073012276652 38-39 7.281939685272098 40.880117576367795 47.02486317943388 4.813079558926223 40-41 5.965304730508778 42.123735364328745 46.797812565972166 5.113147339190308 42-43 5.466196822870783 42.78244418913378 46.6389659106133 5.112393077382136 44-45 6.216292471232521 42.176199668329076 45.32179589450673 6.285711965931673 46-47 5.735836494762077 42.675329894835556 45.34960428858212 6.239229321820252 48-49 6.548828485769903 41.47077151666589 45.98429320434464 5.996106793219568 50-51 6.306783582998417 42.28676978037591 45.85332683798157 5.553119798644095 52-53 5.73354551396292 42.689714246278385 45.82614449663258 5.750595743126119 54-55 4.78258928027735 42.40940035967864 45.832639822028085 6.975370538015922 56-57 5.92556357096752 42.48572043307582 44.596295228777535 6.992420767179121 58-59 6.249568666295889 42.01169979986116 45.05498743550292 6.683744098340025 60-61 5.707442327795313 42.24782048289371 45.382874425307776 6.661862764003207 62-63 6.320992164981935 41.595217797263835 45.76340681200016 6.32038322575407 64-65 5.7948686721065235 42.33081638452482 45.571793934965285 6.302521008403361 66-67 5.4090371191315665 41.99980919788007 45.616727256488794 6.974426426499574 68-69 5.698748812549223 41.61071100898809 45.28754577267524 7.402994405787452 70-71 5.673869711891723 42.35540796732851 44.37362735506291 7.5970949657168605 72-73 6.2257693915909895 40.59943734243762 44.65130251572072 8.523490750250678 74-75 6.26738006990594 41.30925462081508 44.47897341382124 7.944391895457738 76-77 6.333461983790042 40.37193524142274 45.51240122691909 7.782201547868127 78-79 6.107403257498254 39.90313570744223 46.15547003134083 7.83399100371868 80-81 5.609322415773898 40.79673285132606 45.2482635153513 8.345681217548744 82-83 5.289427640045305 40.74253748005732 44.96977643901904 8.998258440878331 84-85 5.832611065215229 39.805667588869426 44.5746669616633 9.787054384252052 86-87 6.367309790282244 39.49044456588648 44.38081152624875 9.761434117582526 88-89 6.7613830879835675 38.5608728256794 45.04919222053765 9.628551865799384 90-91 6.510766748955101 38.464750405331635 45.021753476889984 10.002729368823275 92-93 5.652150967600504 39.20111557213668 45.137234046871896 10.009499413390925 94-95 5.490196078431373 39.1959972394755 44.41014086794138 10.903665814151747 96-97 5.558983643958738 38.49028339916617 44.11219050789385 11.838542448981249 98-99 6.839171683628938 37.65664898043689 43.729575246314916 11.774604089619253 100-101 6.8757218452445 37.2590889069766 43.974863039696665 11.890326208082232 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 33573.0 1 20673.0 2 7506.5 3 6656.0 4 4632.0 5 4325.0 6 9823.5 7 18692.5 8 22524.5 9 19697.5 10 13159.5 11 7652.0 12 6095.0 13 5281.0 14 4110.0 15 2665.0 16 1677.5 17 1246.0 18 1076.0 19 923.0 20 799.5 21 769.0 22 762.5 23 758.5 24 726.0 25 718.5 26 761.5 27 814.5 28 858.5 29 906.5 30 1074.5 31 1189.5 32 1277.0 33 1491.5 34 1672.5 35 1637.5 36 1515.5 37 1390.0 38 1243.5 39 1136.5 40 1063.5 41 989.0 42 895.0 43 902.0 44 1041.0 45 1214.0 46 1825.0 47 3638.5 48 7229.0 49 8678.0 50 8011.5 51 6374.5 52 3321.5 53 1577.0 54 798.5 55 444.5 56 297.5 57 217.5 58 180.5 59 152.5 60 117.5 61 98.0 62 86.5 63 98.5 64 90.5 65 65.0 66 60.0 67 45.5 68 34.0 69 25.5 70 13.5 71 8.0 72 10.0 73 9.0 74 5.0 75 3.5 76 2.5 77 1.0 78 0.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32476626977522116 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001014894593047566 16-17 0.0022327681047046456 18-19 6.089367558285396E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012178735116570793 36-37 0.020500870779560833 38-39 0.009337030256037607 40-41 0.006089367558285396 42-43 0.0032476626977522116 44-45 0.001014894593047566 46-47 0.001014894593047566 48-49 0.0018268102674856188 50-51 4.0595783721902645E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.119156744380529E-4 60-61 0.0014208524302665925 62-63 4.0595783721902645E-4 64-65 4.0595783721902645E-4 66-67 0.001014894593047566 68-69 0.002029789186095132 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.07550815772273892 78-79 0.002841704860533185 80-81 0.0 82-83 0.0 84-85 2.0297891860951322E-4 86-87 0.08707795608348116 88-89 0.39256122859079856 90-91 0.3462820351478295 92-93 0.0 94-95 4.0595783721902645E-4 96-97 0.0 98-99 0.0 100-101 2.0297891860951322E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 246331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.658731544141826 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60090282201114 11.521895335950408 2 9.50178625827356 2.7856826789969595 3 3.650059541942452 1.6051572883640306 4 1.9773463679415106 1.1594155830975394 5 1.1769918856794706 0.8626604040904312 6 0.7366584508017392 0.6479087082015662 7 0.5815724611592678 0.5967580207119688 8 0.40710072281148746 0.4774064165695751 9 0.2935556232518209 0.3872837767069512 >10 2.1961283890442824 6.403578924292923 >50 0.38217618876180454 3.884610544348864 >100 0.3683292254008696 12.1340797544767 >500 0.07477360214904871 7.739992124417958 >1k 0.036002104738430865 10.819588277561493 >5k 0.0027693926721869895 2.3387231002188114 >10k+ 0.013846963360934949 36.63525906199382 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26569 10.785893777072312 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24562 9.971136397773728 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15590 6.328882682244622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12391 5.030223560980956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11132 4.519122643922202 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5761 2.3387231002188114 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4317 1.752519983274537 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 1.3640183330559288 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2544 1.0327567378852032 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2295 0.9316732364176658 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2178 0.8841761694630395 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1870 0.7591411555995795 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1833 0.7441207156224755 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1598 0.6487206238760043 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1587 0.6442550876665949 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1551 0.62964060552671 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1368 0.5553503213156281 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1133 0.45995022956915693 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1018 0.41326507828896886 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 905 0.36739184268321895 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 900 0.3653620534971238 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 891 0.36170843296215255 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 885 0.3592726859388384 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.3393807519151061 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 834 0.3385688362406681 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.32436031193800213 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 798 0.3239543541007831 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 779 0.3162411551936216 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 771 0.3129934924958694 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 770 0.31258753465865036 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 745 0.3024385887281747 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 737 0.29919092603042247 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.2886360222627278 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 686 0.2784870763322521 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 647 0.2626547206807101 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 642 0.26062493149461496 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 622 0.25250577475023445 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 615 0.24966406988970125 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 597 0.24235682881975878 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 588 0.23870320828478755 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 570 0.23139596721484507 RNA PCR Primer, Index 26 (100% over 22bp) CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 568 0.230584051540407 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 559 0.22693043100543578 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 545 0.22124702128436938 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 540 0.21921723209827426 No Hit GAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 526 0.21353382237720792 No Hit GGTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 500 0.2029789186095132 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 496 0.2013550872606371 No Hit GCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 496 0.2013550872606371 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 485 0.19688955105122782 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 481 0.1952657197023517 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 463 0.18795847863240922 No Hit GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 454 0.18430485809743802 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 451 0.18308698458578093 No Hit GTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 435 0.1765916591902765 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 434 0.17618570135305747 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 412 0.16725462893423887 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 400 0.16238313488761058 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 373 0.15142227328269686 No Hit ACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 360 0.14614482139884952 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 355 0.14411503221275437 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 355 0.14411503221275437 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 348 0.1412733273522212 No Hit GTATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 338 0.13721374898003094 No Hit GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 336 0.13640183330559288 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 330 0.13396608628227874 No Hit GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 329 0.1335601284450597 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 329 0.1335601284450597 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 328 0.13315417060784068 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 319 0.12950055007286942 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 313 0.12706480304955528 No Hit TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.12503501386346014 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 300 0.12178735116570794 No Hit GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.12016351981683183 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 296 0.12016351981683183 No Hit ACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 292 0.11853968846795573 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 292 0.11853968846795573 RNA PCR Primer, Index 26 (100% over 24bp) GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 285 0.11569798360742253 No Hit GTACATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 282 0.11448011009576545 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 280 0.1136681944213274 No Hit ATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 279 0.11326223658410838 No Hit GAGTACATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 279 0.11326223658410838 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 275 0.11163840523523227 No Hit GTACATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 275 0.11163840523523227 No Hit GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 273 0.1108264895607942 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 267 0.10839074253748006 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 265 0.107578826863042 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 264 0.10717286902582297 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 260 0.10554903767694687 No Hit GAGTACATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.10473712200250883 No Hit TATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.10473712200250883 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 258 0.10473712200250883 RNA PCR Primer, Index 26 (100% over 29bp) TATCAACGCAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250 0.1014894593047566 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 247 0.10027158579309953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.0595783721902645E-4 0.0 18-19 0.0 0.0 0.0 4.0595783721902645E-4 0.0 20-21 0.0 0.0 0.0 0.0014208524302665925 0.0 22-23 0.0 0.0 0.0 0.004059578372190264 0.0 24-25 0.0 0.0 0.0 0.007916177825771016 0.0 26-27 0.0 0.0 0.0 0.012381714035180305 0.0 28-29 0.0 0.0 0.0 0.019080018349294244 0.0 30-31 0.0 0.0 0.0 0.03410045832639822 0.0 32-33 0.0 0.0 0.0 0.05663111829205419 0.0 34-35 0.0 0.0 0.0 0.09093455553706192 0.0 36-37 0.0 0.0 0.0 0.14675375815467806 0.0 38-39 0.0 0.0 0.0 0.21008318076084617 0.0 40-41 0.0 0.0 0.0 0.28802708550689926 0.0 42-43 0.0 0.0 0.0 0.34465820379895346 0.0 44-45 0.0 0.0 0.0 0.40149230100961714 0.0 46-47 0.0 0.0 0.0 0.4713170490112897 0.0 48-49 0.0 0.0 0.0 0.5460132910595905 0.0 50-51 0.0 0.0 0.0 0.6233482590498151 0.0 52-53 0.0 0.0 0.0 0.6915491757026115 0.0 54-55 0.0 0.0 0.0 0.7562994507390461 0.0 56-57 0.0 0.0 0.0 0.8338373976478803 0.0 58-59 0.0 0.0 0.0 0.9345149412781988 0.0 60-61 0.0 0.0 0.0 1.0792389102467816 0.0 62-63 0.0 0.0 0.0 1.1985905143891755 0.0 64-65 0.0 0.0 0.0 1.2935846482984277 0.0 66-67 0.0 0.0 0.0 1.4040051800220028 0.0 68-69 0.0 0.0 0.0 1.529040193885463 0.0 70-71 0.0 0.0 0.0 1.6910173709358545 0.0 72-73 0.0 0.0 0.0 1.8493409274512749 0.0 74-75 0.0 0.0 0.0 2.0192342823274374 0.0 76-77 0.0 0.0 0.0 2.174919112900934 0.0 78-79 0.0 0.0 0.0 2.331415859148869 0.0 80-81 0.0 0.0 0.0 2.495016867548136 0.0 82-83 0.0 0.0 0.0 2.6831783250991554 0.0 84-85 0.0 0.0 0.0 2.9064551355696198 0.0 86-87 0.0 0.0 0.0 3.143534512505531 0.0 88-89 0.0 0.0 0.0 3.338597253289273 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGATG 20 0.0020790356 71.190315 8 GGGTGTT 20 0.0020790356 71.190315 9 CTGGGTG 25 0.0050332723 56.952255 5 TATGGGG 40 5.402161E-4 47.460213 6 GTAAGCG 35 7.960989E-8 47.460213 94-95 AGTATAT 60 1.4960879E-6 47.46021 2 ATGAGAG 185 0.0 41.04667 5 TGGGAGT 85 3.6490928E-7 39.084877 6 GGGAGTT 50 0.001620007 37.96817 7 GCTTGGT 25 0.0016215815 37.96817 14-15 CATGAGA 215 0.0 37.52668 4 TGAGAGA 215 0.0 37.52668 6 TACATGA 230 0.0 37.142776 2 GTATATG 80 1.08036365E-5 35.595158 3 ACATGAG 230 0.0 35.07929 3 TGGGGAG 230 0.0 33.0158 6 TGGGTGT 60 0.0039611957 31.64014 8 TATATGG 75 3.0866155E-4 31.64014 4 ATATGGG 75 3.0866155E-4 31.64014 5 GGAGTTG 60 0.0039611957 31.64014 8 >>END_MODULE