FastQCFastQC Report
Fri 27 May 2016
SRR939017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013135
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126350.41933069709787313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105330.349569468344432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.19803294575251357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116050.033.7827721
GTATCAA171950.030.9983041
ATCAACG206950.025.5874213
TATCAAC208150.025.4171542
TCAACGC210500.025.1784024
CAACGCA211400.025.02645
AACGCAG215400.024.5477876
GAGTACT107800.023.25233312-13
ACGCAGA234950.022.1022287
GTACTTT113350.021.96819314-15
CGCAGAG233600.021.8655648
GTACATG115750.021.2407171
GCAGAGT236900.020.3620389
AGTACTT111650.020.20335612-13
TACATGG118150.020.1242712
ACATGGG120800.018.6241663
AGAGTAC201300.018.49569310-11
ACTTTTT140300.018.10490616-17
CAGAGTA226150.017.92860410-11
TACTTTT132800.016.89703214-15
Produced by FastQC (version 0.11.5)