##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939017_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3013135 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.651781284277007 33.0 31.0 34.0 30.0 34.0 2 31.75169582511238 33.0 31.0 34.0 30.0 34.0 3 31.783753466074373 34.0 31.0 34.0 30.0 34.0 4 35.320359691816 37.0 35.0 37.0 33.0 37.0 5 35.35616824337443 37.0 35.0 37.0 33.0 37.0 6 35.431233250418586 37.0 35.0 37.0 33.0 37.0 7 35.35241069517297 37.0 35.0 37.0 33.0 37.0 8 35.33727164564482 37.0 35.0 37.0 33.0 37.0 9 36.91496298705501 39.0 37.0 39.0 33.0 39.0 10-11 36.788697983993416 39.0 37.0 39.0 32.0 39.0 12-13 36.665441475406844 39.0 37.0 39.0 32.0 39.0 14-15 37.839354526099896 40.0 38.0 41.0 32.0 41.0 16-17 37.73771288043848 40.0 38.0 41.0 32.0 41.0 18-19 37.64672475677326 40.0 37.0 41.0 32.0 41.0 20-21 37.77899214605387 40.0 38.0 41.0 32.0 41.0 22-23 37.71561662520929 40.0 38.0 41.0 32.0 41.0 24-25 37.62532096968772 40.0 37.5 41.0 32.0 41.0 26-27 37.49767451508147 40.0 37.0 41.0 32.0 41.0 28-29 37.35719292364929 40.0 37.0 41.0 31.0 41.0 30-31 37.150678280262916 40.0 37.0 41.0 31.0 41.0 32-33 36.94113406800558 40.0 36.0 41.0 30.0 41.0 34-35 36.61170873525415 39.5 36.0 41.0 30.0 41.0 36-37 36.33714138264631 39.0 35.0 41.0 30.0 41.0 38-39 36.100961623027175 39.0 35.0 41.0 29.5 41.0 40-41 35.937078823219004 39.0 35.0 41.0 28.5 41.0 42-43 35.716607121818306 39.0 35.0 40.5 27.0 41.0 44-45 35.62016172524629 39.0 35.0 41.0 26.5 41.0 46-47 35.38304838648119 39.0 35.0 41.0 25.5 41.0 48-49 35.127282050090685 39.0 35.0 41.0 24.0 41.0 50-51 34.027233429633924 38.0 33.5 40.0 23.0 40.5 52-53 34.12634531808233 38.0 33.5 39.5 23.0 40.5 54-55 34.60007782591885 38.5 34.0 40.0 23.0 41.0 56-57 34.50039145275602 38.0 34.0 40.0 23.0 41.0 58-59 34.276987423397884 38.0 34.0 40.0 22.5 41.0 60-61 33.96644707256728 38.0 34.0 40.0 22.0 41.0 62-63 33.57109223449995 37.0 33.0 40.0 20.5 41.0 64-65 33.17510250951251 37.0 33.0 40.0 18.5 41.0 66-67 32.76134955785254 36.0 32.0 39.0 17.5 41.0 68-69 32.31349242566297 36.0 32.0 39.0 13.5 40.5 70-71 31.87180544515928 35.0 31.0 38.5 10.0 40.0 72-73 31.420387569757082 35.0 31.0 37.5 7.5 40.0 74-75 30.944356791182607 35.0 31.0 37.0 6.5 39.0 76-77 30.497426102713618 35.0 30.0 36.5 2.0 39.0 78-79 30.08346871281904 34.5 30.0 36.0 2.0 38.5 80-81 29.62105962726529 34.0 29.5 36.0 2.0 37.5 82-83 29.14538213521797 34.0 29.0 35.0 2.0 37.0 84-85 28.72043204171071 34.0 29.0 35.0 2.0 36.5 86-87 28.25175473385693 34.0 28.0 35.0 2.0 36.0 88-89 27.770414037207097 34.0 27.0 35.0 2.0 36.0 90-91 27.303742281709916 33.5 26.0 35.0 2.0 35.0 92-93 26.788150547519443 33.0 25.0 35.0 2.0 35.0 94-95 26.235200878818905 33.0 24.5 35.0 2.0 35.0 96-97 25.570544300205597 32.5 21.5 35.0 2.0 35.0 98-99 24.667630557542225 32.0 10.0 35.0 2.0 35.0 100-101 21.39610621495552 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23039264909701274 1101 2 0.3248409915106798 1101 3 0.34669550633774904 1101 4 0.355182846189777 1101 5 0.24220675371768863 1101 6 0.28048919743787337 1101 7 0.3863538250971317 1101 8 0.26407521667777445 1101 9 0.45240744085697315 1101 10-11 0.24676336883843675 1101 12-13 0.3169673785767557 1101 14-15 0.40271440413515336 1101 16-17 0.46708432028326996 1101 18-19 0.5570522455498548 1101 20-21 0.5326654968463771 1101 22-23 0.620720818307781 1101 24-25 0.6300519603499666 1101 26-27 0.5963821847050212 1101 28-29 0.6426811759211901 1101 30-31 0.6862596196697197 1101 32-33 0.5168317154171049 1101 34-35 0.511688858878955 1101 36-37 0.35822809387926924 1101 38-39 0.6206452377975253 1101 40-41 0.5798299845334682 1101 42-43 0.5460817421801991 1101 44-45 0.6940857751165552 1101 46-47 0.5447097445033435 1101 48-49 0.6166419555336162 1101 50-51 0.6346726511568761 1101 52-53 0.6408365849970252 1101 54-55 0.7222222232444011 1101 56-57 0.7376505830122326 1101 58-59 0.6859225350627369 1101 60-61 0.621687268159846 1101 62-63 0.6208709001799875 1101 64-65 0.6922120410274673 1101 66-67 0.5515601713196574 1101 68-69 0.6013473495797186 1101 70-71 0.5829457043906565 1101 72-73 0.5499686190361146 1101 74-75 0.3547191055401093 1101 76-77 0.20023997204323152 1101 78-79 0.2718033214011477 1101 80-81 0.141141838895134 1101 82-83 0.27885912180479266 1101 84-85 0.2046498203247289 1101 86-87 0.28674840441976457 1101 88-89 0.24498113920377662 1101 90-91 0.26030856022483917 1101 92-93 0.2738590790602089 1101 94-95 0.06137574240296573 1101 96-97 -0.14406576691083828 1101 98-99 -0.01846072541804844 1101 100-101 0.44334792122032596 1102 1 0.2669316414704177 1102 2 0.3797778553647042 1102 3 0.38345741206989103 1102 4 0.32631277678343196 1102 5 0.40150551534787127 1102 6 0.3493192152078777 1102 7 0.3988420669632191 1102 8 0.4236540147308787 1102 9 0.49916467001724385 1102 10-11 0.575528736883193 1102 12-13 0.5561871284577862 1102 14-15 0.6323406931637905 1102 16-17 0.672271882833634 1102 18-19 0.586205262108507 1102 20-21 0.7103056132949561 1102 22-23 0.6482728124242172 1102 24-25 0.6629171271177867 1102 26-27 0.6933197214226539 1102 28-29 0.6795237331133848 1102 30-31 0.6657683273592383 1102 32-33 0.6239442524622021 1102 34-35 0.590066250952745 1102 36-37 0.5913919499941542 1102 38-39 0.6860862569603157 1102 40-41 0.6944587525333006 1102 42-43 0.5288080186441917 1102 44-45 0.7182006829507301 1102 46-47 0.8467405482672987 1102 48-49 0.7237338749122344 1102 50-51 0.6029064060123375 1102 52-53 0.5698277043616713 1102 54-55 0.755081993034814 1102 56-57 0.6439705769579191 1102 58-59 0.4346518804057382 1102 60-61 0.5515327472392215 1102 62-63 0.50925176572418 1102 64-65 0.46327007660530484 1102 66-67 0.4153590782437533 1102 68-69 0.5517780526324501 1102 70-71 0.5574375274241383 1102 72-73 0.5484912419525756 1102 74-75 0.3374176694347426 1102 76-77 0.32503828275291724 1102 78-79 0.2922370661660807 1102 80-81 0.28863662262551415 1102 82-83 0.2538335801544349 1102 84-85 0.1688768349011731 1102 86-87 0.14555741157482416 1102 88-89 0.07141786310023335 1102 90-91 0.04659625962799652 1102 92-93 0.191386593942525 1102 94-95 0.35687377790319275 1102 96-97 0.15461891086414425 1102 98-99 0.017792772914827282 1102 100-101 0.12457023593906058 1103 1 0.3079869496437624 1103 2 0.2714804127289945 1103 3 0.41386474107200755 1103 4 0.4335035692987006 1103 5 0.24452593046333249 1103 6 0.29124636317487784 1103 7 0.21822547694448247 1103 8 0.21869939517227976 1103 9 0.3593038529259758 1103 10-11 0.38927657735816723 1103 12-13 0.390726145904317 1103 14-15 0.4806487205137202 1103 16-17 0.5040127641631713 1103 18-19 0.6478635340018357 1103 20-21 0.5196152952668811 1103 22-23 0.5126276315107745 1103 24-25 0.5090968969306644 1103 26-27 0.5729560695745874 1103 28-29 0.5500315560688236 1103 30-31 0.567998173820051 1103 32-33 0.5296497784129954 1103 34-35 0.5404977909678266 1103 36-37 0.6098701844151648 1103 38-39 0.7290440154341695 1103 40-41 0.6383169416955283 1103 42-43 0.56847271436407 1103 44-45 0.6563324200604939 1103 46-47 0.7390885298718004 1103 48-49 0.7342745469807497 1103 50-51 0.6909958042652562 1103 52-53 0.7690766763060921 1103 54-55 0.8739778978453856 1103 56-57 0.7679180670423449 1103 58-59 0.6905678840069811 1103 60-61 0.7979599803705923 1103 62-63 0.776592812515922 1103 64-65 0.5556775516034804 1103 66-67 0.6844897933811112 1103 68-69 0.6302262361981548 1103 70-71 0.5980417077779272 1103 72-73 0.47813488990741604 1103 74-75 0.5228605241443312 1103 76-77 0.6801309902498183 1103 78-79 0.5739523981854511 1103 80-81 0.49385938827750664 1103 82-83 0.6419126370198995 1103 84-85 0.6077027832672393 1103 86-87 0.7856221364939167 1103 88-89 0.7788619587763144 1103 90-91 0.6124224756534957 1103 92-93 0.5833419678140999 1103 94-95 0.4873975387616447 1103 96-97 0.5013214682284293 1103 98-99 0.37128670191208 1103 100-101 0.47546993664714066 1104 1 0.23393819138447824 1104 2 0.24833163168547046 1104 3 0.19643480258944024 1104 4 0.3373222068015522 1104 5 0.26176903823275666 1104 6 0.3623195005638067 1104 7 0.40826876698777426 1104 8 0.32276410239487774 1104 9 0.49095570984098913 1104 10-11 0.4421041007120081 1104 12-13 0.3297555375652834 1104 14-15 0.4498945623911439 1104 16-17 0.4830717753274456 1104 18-19 0.4831585898232049 1104 20-21 0.47332912529650173 1104 22-23 0.5212446285488213 1104 24-25 0.5088348782475975 1104 26-27 0.47345048743743234 1104 28-29 0.490822168964705 1104 30-31 0.49360081679110834 1104 32-33 0.46531621723733707 1104 34-35 0.46197876613302213 1104 36-37 0.5710686350872933 1104 38-39 0.5609601694555906 1104 40-41 0.6283192201188612 1104 42-43 0.5939454872052679 1104 44-45 0.7744572776590317 1104 46-47 0.7067577028041327 1104 48-49 0.7249831366367161 1104 50-51 0.6343154670585989 1104 52-53 0.6528953138089406 1104 54-55 0.738769422110586 1104 56-57 0.7261796300407539 1104 58-59 0.6123473326285307 1104 60-61 0.662099875563122 1104 62-63 0.6481512540743637 1104 64-65 0.5323145987668454 1104 66-67 0.5990876516631829 1104 68-69 0.5579273070571205 1104 70-71 0.5751959570374403 1104 72-73 0.4614386037374416 1104 74-75 0.5135212669103382 1104 76-77 0.5367748726831714 1104 78-79 0.4893408693633958 1104 80-81 0.5030915596149406 1104 82-83 0.6658087409160061 1104 84-85 0.5799834850216214 1104 86-87 0.6748611431013423 1104 88-89 0.7209991198608456 1104 90-91 0.6163823754028712 1104 92-93 0.6265112546675979 1104 94-95 0.6427017082763449 1104 96-97 0.6645773351685058 1104 98-99 0.7208583583258381 1104 100-101 0.7882177151843948 1105 1 0.1588075495305752 1105 2 0.17515896394574426 1105 3 0.22422127757456067 1105 4 0.16592009448262957 1105 5 0.1554144420649166 1105 6 0.2446249709847237 1105 7 0.1567087819812656 1105 8 0.2419138830047629 1105 9 0.20896995366168625 1105 10-11 0.3155810482127137 1105 12-13 0.3150894735783325 1105 14-15 0.35331991462803813 1105 16-17 0.33110387904715566 1105 18-19 0.32068752947438384 1105 20-21 0.35928705531215854 1105 22-23 0.29752293428384036 1105 24-25 0.37010425403310165 1105 26-27 0.36452935763345096 1105 28-29 0.37806777565004523 1105 30-31 0.40051656657613677 1105 32-33 0.4454222741685143 1105 34-35 0.5234576098699293 1105 36-37 0.582394236480809 1105 38-39 0.665371123526981 1105 40-41 0.5407561041177118 1105 42-43 0.5203119899724484 1105 44-45 0.696966262731685 1105 46-47 0.6021615349651697 1105 48-49 0.6257063523005044 1105 50-51 0.6055345705357666 1105 52-53 0.6656188381623451 1105 54-55 0.6278318876586226 1105 56-57 0.6449865446880736 1105 58-59 0.6393058579741506 1105 60-61 0.6914121592047806 1105 62-63 0.659733729324067 1105 64-65 0.723436465016043 1105 66-67 0.7094530078689445 1105 68-69 0.7559704354058496 1105 70-71 0.6203903212470898 1105 72-73 0.6669068197319774 1105 74-75 0.5762447912637434 1105 76-77 0.5503517033228214 1105 78-79 0.6012981819998267 1105 80-81 0.7436103786566797 1105 82-83 0.8733727388874755 1105 84-85 0.7984894272966621 1105 86-87 0.7251014800140076 1105 88-89 0.8081885009381686 1105 90-91 0.8279516210977533 1105 92-93 0.7396294607399625 1105 94-95 0.6971258919025658 1105 96-97 0.6852716663042635 1105 98-99 0.7879873244361342 1105 100-101 1.0292899706029992 1106 1 0.21998092846210682 1106 2 0.2254219570904077 1106 3 0.19980635711415928 1106 4 0.22721035925623312 1106 5 0.19977672343144803 1106 6 0.3023816067997771 1106 7 0.24088913302031756 1106 8 0.18432812481047023 1106 9 0.2771692006671316 1106 10-11 0.2899220623679284 1106 12-13 0.3233202716681589 1106 14-15 0.2950724970131873 1106 16-17 0.39082763074740257 1106 18-19 0.3871377665699782 1106 20-21 0.4352327351894658 1106 22-23 0.4927790570613695 1106 24-25 0.40751992078243404 1106 26-27 0.45722376127575615 1106 28-29 0.24026792328923108 1106 30-31 0.3810358241255898 1106 32-33 0.44745043429171716 1106 34-35 0.3872714411300038 1106 36-37 0.34805003137679336 1106 38-39 0.4491721447462069 1106 40-41 0.6387468468977531 1106 42-43 0.5083254104556758 1106 44-45 0.5635130375900204 1106 46-47 0.4501157147901722 1106 48-49 0.5080759555071737 1106 50-51 0.39581830484766556 1106 52-53 0.3998207223251242 1106 54-55 0.3111343231271775 1106 56-57 0.3340277744057474 1106 58-59 0.42406572462486736 1106 60-61 0.6143886670003482 1106 62-63 0.5733741683569988 1106 64-65 0.370762224470532 1106 66-67 0.5847476648217125 1106 68-69 0.5820049936126352 1106 70-71 0.520737799385909 1106 72-73 0.48225545690202054 1106 74-75 0.6212292498400807 1106 76-77 0.49323458683064914 1106 78-79 0.6868814521789623 1106 80-81 0.7048837059665196 1106 82-83 0.8119126370199012 1106 84-85 0.7091073672501764 1106 86-87 0.7614341896093428 1106 88-89 0.7962728506638506 1106 90-91 0.8437677438290443 1106 92-93 0.8406299272428015 1106 94-95 0.8707373233610447 1106 96-97 0.9624267851468709 1106 98-99 0.9429188722255013 1106 100-101 1.079627419219765 1107 1 0.16852272303951565 1107 2 0.22471609627748634 1107 3 0.17710420766301738 1107 4 0.25435428098357704 1107 5 0.23245636976913886 1107 6 0.2762321767654612 1107 7 0.08420764935664238 1107 8 0.17385355415463977 1107 9 0.24342382266163298 1107 10-11 0.29224000006587403 1107 12-13 0.32250192970343505 1107 14-15 0.30192466982094857 1107 16-17 0.2697452557695428 1107 18-19 0.24320353494759672 1107 20-21 0.235478656500149 1107 22-23 0.19026441214159462 1107 24-25 0.24607436418370554 1107 26-27 0.27799803770246 1107 28-29 0.3121781962542016 1107 30-31 0.37195961043044434 1107 32-33 0.29989059271290586 1107 34-35 0.3479611427434932 1107 36-37 0.30266821723305526 1107 38-39 0.47104654534384593 1107 40-41 0.3288763591069781 1107 42-43 0.474119496413536 1107 44-45 0.33569178545511136 1107 46-47 0.32983367102657013 1107 48-49 0.3759611928406912 1107 50-51 0.3336361314984302 1107 52-53 0.30874672406700654 1107 54-55 0.41564089968961326 1107 56-57 0.37732211962664053 1107 58-59 0.47066056854851723 1107 60-61 0.2747601175058847 1107 62-63 0.2859835062313394 1107 64-65 0.3067837368779891 1107 66-67 0.24560827354378745 1107 68-69 0.3678108817743606 1107 70-71 0.33571324511183676 1107 72-73 0.3738173744205824 1107 74-75 0.2838658676918797 1107 76-77 0.3360162221976104 1107 78-79 0.40273283985566266 1107 80-81 0.2832425159079044 1107 82-83 0.41046384995790675 1107 84-85 0.37079601162114173 1107 86-87 0.41593660190272175 1107 88-89 0.409642093074325 1107 90-91 0.53880931539436 1107 92-93 0.6428183710864346 1107 94-95 0.6261342390028162 1107 96-97 0.6191663161974077 1107 98-99 0.5724894729907177 1107 100-101 0.875468163345964 1108 1 0.2231713367044037 1108 2 0.2181938305553075 1108 3 0.13897157477667577 1108 4 0.12656370054284594 1108 5 0.13140322084403522 1108 6 0.07125439853044213 1108 7 0.2491348113491938 1108 8 0.25452880213868667 1108 9 0.29163714973496013 1108 10-11 0.28434372127625096 1108 12-13 0.2685485645463359 1108 14-15 0.2917924194557244 1108 16-17 0.3174521398403911 1108 18-19 0.42349607475691187 1108 20-21 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18673.0 15 19631.0 16 23697.0 17 31100.0 18 27819.0 19 20976.0 20 21049.0 21 22365.0 22 24238.0 23 26732.0 24 30144.0 25 33812.0 26 39287.0 27 45474.0 28 52897.0 29 61955.0 30 74130.0 31 88888.0 32 109558.0 33 139017.0 34 185022.0 35 262023.0 36 394734.0 37 585210.0 38 500631.0 39 70692.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89673438182612 19.388425822331616 15.317258308559495 22.397581487282764 2 15.234526948267977 20.551598320344382 39.99124362746352 24.222631103924126 3 18.841373188316723 24.944236732594785 30.372957755462878 25.841432323625618 4 12.95792401577733 17.47467015383961 37.51574809428125 32.05165773610181 5 13.962700872319916 37.09325047739521 34.0773288861731 14.866719764111776 6 30.92094142760317 36.3856926861132 17.845023028267867 14.848342858015759 7 27.64246541769421 33.397604029194795 20.97281435908744 17.987116194023557 8 26.104352452295583 34.569286187993555 20.07359858979762 19.252762769913247 9 26.29546468218768 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29.162638195519484 22.1951959921099 34-35 22.4480849753182 26.630205862031648 28.989269868224994 21.93243929442516 36-37 22.471425490618934 26.416808478025956 29.03552764416682 22.076238387188294 38-39 22.28498938771917 26.60466873659917 29.118484543953716 21.991857331727942 40-41 22.449522936905506 26.525598296069862 29.05813714663722 21.966741620387413 42-43 22.201519418647933 26.634785868566084 28.854045623916868 22.309649088869115 44-45 22.31438866054828 26.82123965712662 28.622198843346037 22.242172838979066 46-47 22.350361525833137 26.937193433498123 28.223963902784604 22.48848113788413 48-49 22.512252733148042 26.92065126176922 28.14246347031697 22.42463253476577 50-51 22.466643004254564 27.14179300148579 28.071498958208934 22.320065036050707 52-53 22.64781571182364 27.178679824929368 27.868427825228924 22.30507663801807 54-55 22.372935007286493 27.119795678004067 27.945359142872295 22.561910171837145 56-57 22.405810208783745 27.431516751021206 27.803417067596687 22.359255972598362 58-59 22.49910059079265 27.078835592664824 27.926397485188502 22.495666331354023 60-61 22.510789903060434 26.8892933198839 28.201896129966443 22.398020647089222 62-63 22.4613497865579 26.912849117664816 28.224853472223842 22.400947623553442 64-65 22.35142543411551 26.93323454188831 28.157496737763925 22.557843286232256 66-67 22.239747449884753 27.106665356584074 27.996909851025926 22.656677342505247 68-69 22.16464607940002 27.121816836792405 28.115743563100683 22.59779352070689 70-71 22.252330168161834 26.967337939281826 27.980653281254487 22.799678611301857 72-73 22.354007810271458 26.931640941035955 27.88965864676454 22.824692601928046 74-75 22.238366564729322 27.18537493836049 27.735993401987013 22.840265094923172 76-77 22.344165710911156 27.00310483647033 27.853753646727746 22.798975805890766 78-79 22.33721062074393 26.9839194840362 27.843103252786243 22.83576664243362 80-81 22.154624604213762 27.238597654397488 27.775856422392547 22.830921318996207 82-83 22.294878948753468 27.179070860348215 27.72703425029676 22.799015940601556 84-85 22.3052275690859 26.915996235657953 27.727217617227517 23.05155857802863 86-87 22.262906285198543 26.86376127312285 27.981426009390393 22.891906432288216 88-89 22.339311297200926 26.74708259463534 27.852119194584308 23.061486913579422 90-91 22.150172424664085 26.817448034905944 28.0265724472159 23.00580709321407 92-93 22.250480550046518 26.776367029971343 27.872202963521236 23.100949456460906 94-95 22.091378095114962 26.79577767304909 27.834643600405386 23.27820063143056 96-97 22.180348846073585 26.587206597458827 27.824296852284974 23.408147704182614 98-99 22.032172181055678 26.512456169031566 27.83095508973046 23.624416560182297 100-101 22.420763909991123 25.85008327298696 28.39597180893344 23.33318100808847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2583.0 1 1551.0 2 553.5 3 637.0 4 620.0 5 613.0 6 1064.5 7 2235.0 8 3840.0 9 4785.0 10 4431.5 11 3868.5 12 3903.0 13 4209.5 14 4526.5 15 4852.0 16 5281.0 17 5471.0 18 5565.5 19 5853.5 20 6190.0 21 6806.5 22 7883.5 23 9372.5 24 11272.5 25 13693.0 26 17354.0 27 21548.5 28 25440.0 29 29784.0 30 34312.5 31 40033.0 32 45410.5 33 50170.5 34 56320.0 35 61838.0 36 68725.0 37 76007.5 38 79833.5 39 82177.0 40 84970.0 41 89621.5 42 95119.5 43 99844.5 44 104385.5 45 107773.5 46 109047.5 47 111815.5 48 122818.0 49 124909.5 50 116750.5 51 112389.0 52 107379.0 53 104467.0 54 101499.0 55 94665.5 56 85648.5 57 76564.0 58 68439.0 59 59898.0 60 51067.0 61 41776.0 62 33137.0 63 26307.0 64 19923.5 65 14875.5 66 10874.0 67 8044.0 68 5909.0 69 4096.5 70 2947.0 71 2001.0 72 1446.5 73 1052.5 74 740.0 75 528.5 76 345.0 77 197.0 78 120.0 79 92.0 80 63.0 81 45.0 82 44.0 83 36.0 84 26.5 85 21.0 86 15.5 87 12.5 88 8.5 89 7.5 90 7.5 91 7.5 92 8.0 93 5.0 94 3.5 95 4.0 96 4.5 97 3.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3307850461396519 2 0.09146619716673829 3 0.10251780952396757 4 0.10776151748925952 5 0.11861400169590809 6 0.06093321407769648 7 0.0634886920101489 8 0.06425201658737495 9 0.06750444304685983 10-11 0.0645673028257944 12-13 0.06265899138272928 14-15 0.06055155178908347 16-17 0.06232711113176144 18-19 0.07015948505460261 20-21 0.07234989471099038 22-23 0.0888609371966407 24-25 0.09767235785983701 26-27 0.09217973970631917 28-29 0.08987317196209264 30-31 0.08426439572073605 32-33 0.07546956907008813 34-35 0.3288933287091351 36-37 0.5082580103447074 38-39 0.4807783255645698 40-41 0.4630061381252416 42-43 0.49393737751544486 44-45 0.5036946568938996 46-47 0.4981190686776397 48-49 0.5156921279663872 50-51 0.5115270308167408 52-53 0.5102161038254177 54-55 0.5018693155135764 56-57 0.48062897945163424 58-59 0.4627738219495641 60-61 0.4234957942475196 62-63 0.4944351978918966 64-65 0.5168868968698714 66-67 0.526644176248326 68-69 0.5387743994212009 70-71 0.5356879130871999 72-73 0.5243707965291964 74-75 0.5266607702608745 76-77 0.500160132221092 78-79 0.48039666327595676 80-81 0.4869678922451201 82-83 0.4958954709961552 84-85 0.49787015848941385 86-87 0.49662560754828444 88-89 0.5037942209691899 90-91 0.5175340633592587 92-93 0.5239891342405832 94-95 0.5300957308583917 96-97 0.522329732985744 98-99 0.525200497156616 100-101 0.5032632125676413 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3013135.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.02160365042087 #Duplication Level Percentage of deduplicated Percentage of total 1 75.22707081898534 40.638870035653675 2 13.460004273195834 14.54262031959113 3 4.71462175166748 7.640742828907007 4 2.1804278049853636 4.711608266771059 5 1.1689871518766188 3.1575280295556523 6 0.7126420586880662 2.309884010344001 7 0.4812630544825222 1.8199021386592005 8 0.3438470685661603 1.4860136043552161 9 0.2542764490477436 1.2362779393284278 >10 1.3214131625549055 12.822218597680385 >50 0.08907504103177297 3.332350502872227 >100 0.04353031503088749 4.11648936089489 >500 0.002223430346737751 0.8031240535121309 >1k 4.3233367839982316E-4 0.410286921344058 >5k 6.176195405711759E-5 0.19908774983003938 >10k+ 1.2352390811423518E-4 0.7729956407009136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12635 0.41933069709787313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10533 0.349569468344432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5967 0.19803294575251357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.637605019356915E-5 0.0 0.0 0.0 3.3188025096784576E-5 7 9.956407529035373E-5 0.0 0.0 0.0 3.3188025096784576E-5 8 9.956407529035373E-5 0.0 0.0 0.0 3.3188025096784576E-5 9 9.956407529035373E-5 0.0 0.0 0.0 3.3188025096784576E-5 10-11 9.956407529035373E-5 0.0 0.0 0.0 3.3188025096784576E-5 12-13 1.8253413803231516E-4 0.0 0.0 3.3188025096784576E-5 3.3188025096784576E-5 14-15 3.318802509678458E-4 0.0 0.0 6.637605019356915E-5 3.3188025096784576E-5 16-17 3.318802509678458E-4 0.0 0.0 9.956407529035373E-5 3.3188025096784576E-5 18-19 3.8166228861302265E-4 0.0 0.0 1.493461129355306E-4 3.3188025096784576E-5 20-21 3.982563011614149E-4 0.0 0.0 2.3231617567749205E-4 3.3188025096784576E-5 22-23 3.982563011614149E-4 0.0 0.0 2.986922258710612E-4 4.9782037645176864E-5 24-25 4.314443262581995E-4 0.0 0.0 4.646323513549841E-4 6.637605019356915E-5 26-27 4.314443262581995E-4 0.0 0.0 7.46730564677653E-4 6.637605019356915E-5 28-29 4.646323513549841E-4 0.0 0.0 0.0018087473677747595 6.637605019356915E-5 30-31 4.978203764517687E-4 0.0 0.0 0.004978203764517687 6.637605019356915E-5 32-33 5.310084015485532E-4 0.0 0.0 0.01144986865839068 6.637605019356915E-5 34-35 5.641964266453379E-4 0.0 0.0 0.02289973731678136 6.637605019356915E-5 36-37 5.641964266453379E-4 0.0 0.0 0.042961898487787635 6.637605019356915E-5 38-39 6.969485270324761E-4 0.0 0.0 0.07183548032199022 6.637605019356915E-5 40-41 7.135425395808684E-4 0.0 0.0 0.10354663830196789 6.637605019356915E-5 42-43 8.131066148712222E-4 0.0 0.0 0.1368674154991396 6.637605019356915E-5 44-45 9.624527278067527E-4 0.0 0.0 0.18210269370605697 6.637605019356915E-5 46-47 0.001095204828193891 0.0 0.0 0.23424108113310554 6.637605019356915E-5 48-49 0.001095204828193891 0.0 0.0 0.29711579467896393 6.637605019356915E-5 50-51 0.001095204828193891 0.0 0.0 0.3644045155626947 6.637605019356915E-5 52-53 0.001095204828193891 0.0 0.0 0.4302329633421669 6.637605019356915E-5 54-55 0.001095204828193891 0.0 0.0 0.5093200271478046 6.637605019356915E-5 56-57 0.0011283928532906757 0.0 0.0 0.6025617836572208 6.637605019356915E-5 58-59 0.0011283928532906757 0.0 0.0 0.7116508221503517 6.637605019356915E-5 60-61 0.0011283928532906757 0.0 0.0 0.8342473868578739 6.637605019356915E-5 62-63 0.0011283928532906757 0.0 0.0 0.9583540067072999 9.956407529035373E-5 64-65 0.0011283928532906757 0.0 0.0 1.0932135466880841 9.956407529035373E-5 66-67 0.0011615808783874602 0.0 0.0 1.252300344989521 1.327521003871383E-4 68-69 0.0011781748909358526 0.0 0.0 1.4347681069716425 1.659401254839229E-4 70-71 0.001211362916032637 0.0 0.0 1.6388744613168678 1.659401254839229E-4 72-73 0.0012777389662262063 0.0 0.0 1.8507136255096437 1.9912815058070746E-4 74-75 0.0013275210038713832 0.0 0.0 2.069970313311551 1.9912815058070746E-4 76-77 0.0013275210038713832 0.0 0.0 2.312425430656111 1.9912815058070746E-4 78-79 0.0013441150164197756 0.0 0.0 2.5957847889324572 1.9912815058070746E-4 80-81 0.001493461129355306 0.0 0.0 2.9111705914272012 2.1572216312909975E-4 82-83 0.001493461129355306 0.0 0.0 3.232895306715431 2.3231617567749205E-4 84-85 0.0015432431670004828 0.0 0.0 3.56938869317173 2.3231617567749205E-4 86-87 0.0015930252046456597 0.0 0.0 3.9280682744052293 2.3231617567749205E-4 88-89 0.0015930252046456597 0.0 0.0 4.3233376533079335 2.3231617567749205E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11605 0.0 33.782772 1 GTATCAA 17195 0.0 30.998304 1 ATCAACG 20695 0.0 25.587421 3 TATCAAC 20815 0.0 25.417154 2 TCAACGC 21050 0.0 25.178402 4 CAACGCA 21140 0.0 25.0264 5 AACGCAG 21540 0.0 24.547787 6 GAGTACT 10780 0.0 23.252333 12-13 ACGCAGA 23495 0.0 22.102228 7 GTACTTT 11335 0.0 21.968193 14-15 CGCAGAG 23360 0.0 21.865564 8 GTACATG 11575 0.0 21.240717 1 GCAGAGT 23690 0.0 20.362038 9 AGTACTT 11165 0.0 20.203356 12-13 TACATGG 11815 0.0 20.124271 2 ACATGGG 12080 0.0 18.624166 3 AGAGTAC 20130 0.0 18.495693 10-11 ACTTTTT 14030 0.0 18.104906 16-17 CAGAGTA 22615 0.0 17.928604 10-11 TACTTTT 13280 0.0 16.897032 14-15 >>END_MODULE