FastQCFastQC Report
Fri 27 May 2016
SRR939017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013135
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123210.4089096572174828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110300.3660639168175339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61290.2034094058181927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118300.034.4798471
GTATCAA171600.029.6294381
ATCAACG205900.024.5450613
TATCAAC208350.024.3247622
TCAACGC208600.024.2046154
CAACGCA210750.024.002725
AACGCAG214600.023.5721026
CTATGCG1503.057433E-522.1458349
GAGTACT114500.021.86389212-13
ACGCAGA238400.020.9401767
CGCAGAG238150.020.7429738
GTACTTT122350.020.40308614-15
GTACATG116850.020.1045551
AGTACTT120000.019.45782912-13
GCAGAGT246050.019.3640239
TACATGG119850.019.1240522
ACATGGG120500.018.19393
AGAGTAC208450.018.07696510-11
GTACCGT3451.0986696E-917.8811366
CAGAGTA237450.017.47811310-11
Produced by FastQC (version 0.11.5)