##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939017_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3013135 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20766643379736 33.0 31.0 34.0 31.0 34.0 2 32.46115192316309 34.0 31.0 34.0 31.0 34.0 3 32.54086922756531 34.0 31.0 34.0 31.0 34.0 4 36.08828711624272 37.0 35.0 37.0 35.0 37.0 5 36.01536008177529 37.0 35.0 37.0 35.0 37.0 6 36.05038207713893 37.0 35.0 37.0 35.0 37.0 7 35.99316094366831 37.0 35.0 37.0 35.0 37.0 8 35.96317390359211 37.0 35.0 37.0 35.0 37.0 9 37.64304818735304 39.0 37.0 39.0 35.0 39.0 10-11 37.55944887965524 39.0 37.0 39.0 35.0 39.0 12-13 37.46620413622357 39.0 37.0 39.0 34.0 39.0 14-15 38.79265084372257 40.0 38.0 41.0 34.5 41.0 16-17 38.87566969286142 40.0 38.0 41.0 35.0 41.0 18-19 38.918307676224266 40.0 38.0 41.0 35.0 41.0 20-21 38.896921810672275 40.0 38.0 41.0 35.0 41.0 22-23 38.82662276997213 40.0 38.0 41.0 34.5 41.0 24-25 38.756528997207226 40.0 38.0 41.0 34.0 41.0 26-27 38.60035693720992 40.0 38.0 41.0 34.0 41.0 28-29 38.44517537382161 40.0 38.0 41.0 34.0 41.0 30-31 38.24745522520564 40.0 38.0 41.0 34.0 41.0 32-33 38.14200176892174 40.0 38.0 41.0 33.0 41.0 34-35 38.047310691356344 40.0 37.5 41.0 33.0 41.0 36-37 37.92095325964486 40.0 37.0 41.0 33.0 41.0 38-39 37.78230563847953 40.0 37.0 41.0 33.0 41.0 40-41 37.60729041347301 40.0 37.0 41.0 33.0 41.0 42-43 37.42261896662446 40.0 37.0 41.0 32.0 41.0 44-45 37.16218788736648 40.0 36.5 41.0 31.0 41.0 46-47 36.83550720429055 40.0 36.0 41.0 31.0 41.0 48-49 36.49015427453466 39.0 36.0 41.0 30.0 41.0 50-51 36.213020160065845 39.0 35.0 41.0 30.0 41.0 52-53 35.937574652313955 39.0 35.0 40.0 29.0 41.0 54-55 35.630105521325795 38.5 35.0 40.0 28.0 41.0 56-57 35.49184835727573 38.0 35.0 40.0 28.0 41.0 58-59 35.2109563627252 38.0 34.5 40.0 27.5 41.0 60-61 34.84908508911815 38.0 34.0 40.0 26.5 41.0 62-63 34.42037960463105 37.0 34.0 40.0 26.0 41.0 64-65 34.00024360010421 37.0 33.0 39.5 26.0 41.0 66-67 33.54233215571158 36.0 33.0 39.0 25.0 40.5 68-69 33.0542901330342 35.5 32.0 39.0 23.0 40.0 70-71 32.52120167201271 35.0 32.0 38.0 22.0 40.0 72-73 32.001061353042594 35.0 31.0 37.5 20.5 39.5 74-75 31.46361945282903 35.0 31.0 37.0 19.5 39.0 76-77 29.58819999767684 32.5 28.5 35.0 16.5 37.0 78-79 30.531814870558406 34.0 30.0 36.0 18.0 38.0 80-81 30.50118979069972 34.0 30.0 35.5 17.0 37.0 82-83 30.080950571414824 34.0 30.0 35.0 10.5 37.0 84-85 29.61556302654876 34.0 29.5 35.0 7.0 36.0 86-87 28.896214241977212 34.0 29.0 35.0 2.0 36.0 88-89 28.19207403584639 33.0 27.5 35.0 2.0 35.0 90-91 27.604387954738172 33.0 26.5 35.0 2.0 35.0 92-93 27.17313230240265 33.0 26.0 34.5 2.0 35.0 94-95 26.602587836256923 32.0 25.0 34.0 2.0 35.0 96-97 25.724554824128358 32.0 23.5 34.0 2.0 35.0 98-99 24.4792626284584 32.0 10.5 34.0 2.0 35.0 100-101 18.907952846453945 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12136142317038434 1101 2 0.1161516539140095 1101 3 0.14878497323729079 1101 4 0.1433153775050755 1101 5 0.12930332525881028 1101 6 0.13075916663825637 1101 7 0.10024982589390419 1101 8 0.10096220693023383 1101 9 0.12990991653504835 1101 10-11 0.25873725633074685 1101 12-13 0.23760835391932034 1101 14-15 0.2545717808735404 1101 16-17 0.21895800612426797 1101 18-19 0.2754503379299038 1101 20-21 0.3078770774908577 1101 22-23 0.23630201326212585 1101 24-25 0.2293286968112227 1101 26-27 0.2676068926127897 1101 28-29 0.26762757843759033 1101 30-31 0.1346364129486659 1101 32-33 0.3062869718438961 1101 34-35 0.25666404643761354 1101 36-37 0.36470410548999865 1101 38-39 0.3556145899374812 1101 40-41 0.32935147637086715 1101 42-43 0.24867726655321576 1101 44-45 0.13825151483768394 1101 46-47 0.14094064049189825 1101 48-49 0.1509468106778442 1101 50-51 0.11644302434391562 1101 52-53 0.061709804589781925 1101 54-55 0.013228278680934125 1101 56-57 0.07927893022498722 1101 58-59 0.2549073839324336 1101 60-61 0.1201005911631583 1101 62-63 0.19232515640061365 1101 64-65 0.0872675153916802 1101 66-67 -0.026413077309712207 1101 68-69 0.11871593337949804 1101 70-71 0.07683550945334616 1101 72-73 0.03142097326453097 1101 74-75 0.20952189379897135 1101 76-77 -0.1567414826872202 1101 78-79 0.12142891718788462 1101 80-81 0.13426371024225503 1101 82-83 0.08559697161630453 1101 84-85 0.18203978554211275 1101 86-87 0.49874802333362567 1101 88-89 0.6200715886836647 1101 90-91 0.686042414307213 1101 92-93 0.43289979517147614 1101 94-95 0.17337193518026695 1101 96-97 0.4508724641141484 1101 98-99 0.5401347513727153 1101 100-101 0.8101289835423202 1102 1 0.18774727721962137 1102 2 0.1835266708326273 1102 3 0.1416473796634108 1102 4 0.11766918345353616 1102 5 0.12139632888412422 1102 6 0.08335514915253128 1102 7 0.14197719339628634 1102 8 0.1720613808167215 1102 9 0.19763409969948498 1102 10-11 0.2842531952423002 1102 12-13 0.2167265387496613 1102 14-15 0.34450106955434734 1102 16-17 0.3644978105911676 1102 18-19 0.27884740168312305 1102 20-21 0.319316425894975 1102 22-23 0.27272257533367394 1102 24-25 0.3527607839157696 1102 26-27 0.2859808718563812 1102 28-29 0.3459014576976145 1102 30-31 0.28983043974387357 1102 32-33 0.29637800005420445 1102 34-35 0.41144699334736146 1102 36-37 0.3459170452345006 1102 38-39 0.35667585399934154 1102 40-41 0.3866292943328773 1102 42-43 0.3368518705363499 1102 44-45 0.2959539233207096 1102 46-47 0.36869411772198646 1102 48-49 0.29577300132381623 1102 50-51 0.26337330849415963 1102 52-53 0.17490243083903323 1102 54-55 0.3039528999101364 1102 56-57 0.34811729208589526 1102 58-59 0.2245301024663604 1102 60-61 0.16216481495168011 1102 62-63 0.2669186002737547 1102 64-65 0.19477651803510554 1102 66-67 0.061443772048882295 1102 68-69 0.01057924407220412 1102 70-71 0.012158010992393997 1102 72-73 0.13722701729707865 1102 74-75 0.18075849049655446 1102 76-77 -0.17743179611941073 1102 78-79 0.19969357913820218 1102 80-81 0.19196649469449412 1102 82-83 0.21385801941096716 1102 84-85 0.529456864925951 1102 86-87 0.6868511203982024 1102 88-89 0.49167410044907456 1102 90-91 0.6040409215881581 1102 92-93 0.7056681874640596 1102 94-95 0.9071748176755747 1102 96-97 0.9175680804101596 1102 98-99 1.0785406734456977 1102 100-101 1.1630002240321176 1103 1 0.1364756515535106 1103 2 0.1541476407331075 1103 3 0.10749744171173603 1103 4 0.1135423615443969 1103 5 0.06943292522934996 1103 6 0.11837206036259573 1103 7 0.1184803444778737 1103 8 0.17419097665096928 1103 9 0.19379975656008241 1103 10-11 0.1478700042551182 1103 12-13 0.10219092787782103 1103 14-15 0.14846364589812566 1103 16-17 0.1949210774962964 1103 18-19 0.2271601739049416 1103 20-21 0.24541504623223176 1103 22-23 0.19304121173048827 1103 24-25 0.141261282735627 1103 26-27 0.1498607171005517 1103 28-29 0.11730228987514835 1103 30-31 0.07132811597328015 1103 32-33 0.13687219671088968 1103 34-35 0.21119673177108922 1103 36-37 0.18584988704777317 1103 38-39 0.16418236299026034 1103 40-41 0.21130652138108275 1103 42-43 0.2764644943288346 1103 44-45 0.2657136381417402 1103 46-47 0.4147572123517662 1103 48-49 0.4502014267563297 1103 50-51 0.27509253374174847 1103 52-53 0.39171643186100624 1103 54-55 0.4635012854370686 1103 56-57 0.5186127053763983 1103 58-59 0.4058473519006256 1103 60-61 0.3735066403890599 1103 62-63 0.35374063107892084 1103 64-65 0.36845342875852083 1103 66-67 0.404542538382195 1103 68-69 0.4154709636624361 1103 70-71 0.36213051506812377 1103 72-73 0.5573816514552874 1103 74-75 0.39155112742869136 1103 76-77 0.16344150363483223 1103 78-79 0.40004031977620613 1103 80-81 0.4998042930739395 1103 82-83 0.4642475855593453 1103 84-85 0.4586705544896681 1103 86-87 0.5386721778963199 1103 88-89 0.3269092757660097 1103 90-91 0.43284144230743493 1103 92-93 0.5019176703946862 1103 94-95 0.48645140720765667 1103 96-97 0.3718612161621593 1103 98-99 0.603448939255852 1103 100-101 0.7498114267963096 1104 1 0.04655083952344086 1104 2 0.07467167809925002 1104 3 0.0482629919509705 1104 4 0.00969182611490993 1104 5 0.054390774397731434 1104 6 0.056722481870913555 1104 7 0.02946234493355604 1104 8 0.06260747243588582 1104 9 0.09035022363686807 1104 10-11 0.1293646499602943 1104 12-13 0.09070539586597448 1104 14-15 0.13789176164203099 1104 16-17 0.1417517906428003 1104 18-19 0.14935885241940383 1104 20-21 0.14186070583578214 1104 22-23 0.032521731211005545 1104 24-25 0.16249390975772826 1104 26-27 0.045294756745121845 1104 28-29 0.09283885856961405 1104 30-31 0.019945113011331728 1104 32-33 0.09284622268491205 1104 34-35 0.10262302477633511 1104 36-37 0.10749718711612388 1104 38-39 0.1107214379503958 1104 40-41 0.27746737806825195 1104 42-43 0.2180525553905852 1104 44-45 0.2962832439745071 1104 46-47 0.2998312611100218 1104 48-49 0.2503176263462237 1104 50-51 0.18771499899579425 1104 52-53 0.20656947355615074 1104 54-55 0.4376935843662082 1104 56-57 0.27101644199066754 1104 58-59 0.2639266410325405 1104 60-61 0.24678529156244622 1104 62-63 0.2662491751664078 1104 64-65 0.3837302571932426 1104 66-67 0.2975227160992091 1104 68-69 0.2789888492855894 1104 70-71 0.3937800935598048 1104 72-73 0.4743012231117021 1104 74-75 0.35570241473787334 1104 76-77 0.2398962769317059 1104 78-79 0.5312091509450347 1104 80-81 0.4947872048989552 1104 82-83 0.5904448970198182 1104 84-85 0.6932934754283764 1104 86-87 0.5993397559323199 1104 88-89 0.5776110301519743 1104 90-91 0.7274233516261859 1104 92-93 0.830562008512711 1104 94-95 1.0214001426827117 1104 96-97 0.7403346725303521 1104 98-99 0.9424532682601843 1104 100-101 1.1771502283456385 1105 1 -0.09846492569606369 1105 2 0.018516627716692824 1105 3 0.03350762846894639 1105 4 0.05213885277246533 1105 5 0.031142036717753285 1105 6 0.06854749895909151 1105 7 0.08559975590967639 1105 8 0.05216494382697334 1105 9 0.011502076877007994 1105 10-11 0.07862269242716735 1105 12-13 0.05808792844376143 1105 14-15 -0.03291722563555055 1105 16-17 -0.08172295193211454 1105 18-19 0.0196276328749434 1105 20-21 0.060864961342581125 1105 22-23 0.012786542800100165 1105 24-25 -0.06611279762655897 1105 26-27 -0.12974879053950872 1105 28-29 0.07889821517226636 1105 30-31 -0.015151793477762965 1105 32-33 -0.07837398325515466 1105 34-35 0.0015249717258214446 1105 36-37 -0.009124646059184727 1105 38-39 0.035563234523934284 1105 40-41 0.05094432556897743 1105 42-43 0.1214503460549281 1105 44-45 0.019765703789730082 1105 46-47 0.093291451174629 1105 48-49 0.018979015890458584 1105 50-51 0.0768469197066608 1105 52-53 0.07302373237033777 1105 54-55 0.12011701831765009 1105 56-57 0.11824485025472597 1105 58-59 0.05289319230809042 1105 60-61 -0.06604627415536157 1105 62-63 -0.003220319685084405 1105 64-65 -0.07670786156405995 1105 66-67 -0.10031314922392198 1105 68-69 -0.08017702728266585 1105 70-71 0.0965560951473492 1105 72-73 0.2082758223664385 1105 74-75 0.17945718288992296 1105 76-77 -0.05620761917218786 1105 78-79 -0.027718593636361533 1105 80-81 0.08474487032351163 1105 82-83 0.03421928901152782 1105 84-85 0.049547747472121983 1105 86-87 0.0998832701430672 1105 88-89 0.32599812692616936 1105 90-91 0.16244529063793678 1105 92-93 0.32119327877046544 1105 94-95 0.3005261100939087 1105 96-97 0.41390422691485185 1105 98-99 0.44548063138941174 1105 100-101 0.48652336793949047 1106 1 -0.004159439749820137 1106 2 0.04062076941794146 1106 3 0.03681199469275498 1106 4 0.008958378604411621 1106 5 0.020393626266930198 1106 6 0.05186739215479719 1106 7 0.039884211653472335 1106 8 0.0406680477761725 1106 9 0.030688812704021018 1106 10-11 0.13676056569447326 1106 12-13 0.028259956424683708 1106 14-15 0.010741659624976307 1106 16-17 -0.035594665384905966 1106 18-19 -0.08605219818975485 1106 20-21 0.017380711160633666 1106 22-23 0.024649010594352205 1106 24-25 0.07915225633919931 1106 26-27 0.010824643771165654 1106 28-29 0.01908642334468169 1106 30-31 0.0926031370294993 1106 32-33 0.21857103010694345 1106 34-35 0.09789977641027292 1106 36-37 0.08602944728647088 1106 38-39 0.10355784459519413 1106 40-41 0.2168933177898822 1106 42-43 0.25491565286870355 1106 44-45 0.3435694881856435 1106 46-47 0.4870638983722344 1106 48-49 0.36701002127633586 1106 50-51 0.3870219998994813 1106 52-53 0.37268573458666054 1106 54-55 0.3686669575335415 1106 56-57 0.3723633997835094 1106 58-59 0.3908565807951305 1106 60-61 0.34823721043009925 1106 62-63 0.4955385630897453 1106 64-65 0.4275380657852068 1106 66-67 0.3382661733122134 1106 68-69 0.2710201276858797 1106 70-71 0.4003107204334242 1106 72-73 0.47175847707398333 1106 74-75 0.5486586306095198 1106 76-77 0.2379288967857356 1106 78-79 0.3780280623706851 1106 80-81 0.42122805165322674 1106 82-83 0.4409470461618241 1106 84-85 0.6013066134829081 1106 86-87 0.6765507865732729 1106 88-89 0.6560297531596966 1106 90-91 0.8214063940125413 1106 92-93 0.7318755579634555 1106 94-95 0.6352163481606539 1106 96-97 0.5960016745604548 1106 98-99 0.6668421260020843 1106 100-101 1.1255336997923848 1107 1 0.057935433082811016 1107 2 0.06323710968851515 1107 3 0.019516167772167137 1107 4 0.04021517565514188 1107 5 0.024995889243157876 1107 6 -0.01935858701055082 1107 7 0.02525317277503092 1107 8 0.09641304124641437 1107 9 -0.037145294747432445 1107 10-11 -0.0059785321803076386 1107 12-13 0.0757306734682146 1107 14-15 0.044946598562802365 1107 16-17 0.011563272606714747 1107 18-19 0.09951216236665772 1107 20-21 0.1050405250235471 1107 22-23 0.08955029103252343 1107 24-25 0.04971437300848436 1107 26-27 0.08079382818014125 1107 28-29 0.0463993249155763 1107 30-31 0.009214222749655221 1107 32-33 0.06960308052419606 1107 34-35 0.02137358900852604 1107 36-37 0.10341940045392306 1107 38-39 0.07287354850121375 1107 40-41 0.09575478827473205 1107 42-43 0.10783785698075121 1107 44-45 -0.05245335954113983 1107 46-47 -0.05465003217464215 1107 48-49 -0.03660734044468228 1107 50-51 -0.10480473301003457 1107 52-53 -0.033380745043523063 1107 54-55 -0.07874572359495602 1107 56-57 0.0030383922350551984 1107 58-59 -0.12248858227443549 1107 60-61 -0.1329163746958244 1107 62-63 -0.11934065704705432 1107 64-65 -0.19563544909649266 1107 66-67 -0.2518870970270868 1107 68-69 -0.29544063136285814 1107 70-71 -0.07923268065704292 1107 72-73 -0.09001076827328092 1107 74-75 -0.09745180442925516 1107 76-77 -0.10374450383667977 1107 78-79 -0.07371304949603186 1107 80-81 -0.0916902451584356 1107 82-83 -0.15228537419853794 1107 84-85 0.18537729776294398 1107 86-87 0.15160297186236704 1107 88-89 0.3504688823295865 1107 90-91 0.3752787856658273 1107 92-93 0.3193402362432707 1107 94-95 0.505876502965922 1107 96-97 0.744498706113454 1107 98-99 0.7007160457012098 1107 100-101 0.9660008390060781 1108 1 -0.05381544746398248 1108 2 0.015001336387925335 1108 3 -0.004295538309449398 1108 4 0.005741979256264074 1108 5 -0.01486720747575987 1108 6 0.08241213974216777 1108 7 0.03937348642364924 1108 8 0.09062425276282937 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21.0 5 64.0 6 136.0 7 361.0 8 861.0 9 1586.0 10 2689.0 11 4153.0 12 5969.0 13 8178.0 14 10280.0 15 14079.0 16 19057.0 17 27259.0 18 27723.0 19 19564.0 20 18068.0 21 19465.0 22 20739.0 23 23109.0 24 26655.0 25 31169.0 26 37652.0 27 44829.0 28 54195.0 29 65443.0 30 79747.0 31 96217.0 32 118628.0 33 151656.0 34 203112.0 35 290662.0 36 452551.0 37 672377.0 38 441688.0 39 23177.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.692524541655835 20.13768651991656 15.891863433099413 22.277925505328188 2 15.612078449853723 21.0868414458695 39.308228804882624 23.992851299394154 3 18.84346370142725 25.021746453444667 30.670281948867213 25.46450789626087 4 13.329505647772171 17.996505300957306 37.12372661696207 31.55026243430845 5 13.886998093347957 37.27323867002308 34.15339837079985 14.68636486582911 6 30.95161683761265 36.712294669837235 17.5486329022762 14.787455590273918 7 27.67954306727047 33.44124972827304 20.86763454010524 18.01157266435125 8 26.10981585624275 34.96594078924443 19.79781855111039 19.126424803402436 9 26.045497463605184 17.222925623976355 20.69226901549383 36.03930789692463 10-11 24.726489188171126 27.053567131907464 27.075703544647023 21.14424013527439 12-13 25.79150950753949 24.42061175486661 27.670515924444146 22.11736281314976 14-15 22.920668625220205 25.608657240298054 26.330851731833043 25.139822402648694 16-17 21.90871675017532 28.83142757481511 26.989463538906115 22.27039213610345 18-19 21.784267102582554 27.348035814709636 29.45980071484055 21.407896367867256 20-21 22.737713378258857 26.4550708813246 29.383001425425675 21.424214314990863 22-23 22.438921588312503 26.353050892177084 29.2434125918686 21.964614927641808 24-25 22.260421670802728 26.59240839890048 29.319852346353837 21.827317583942957 26-27 22.1570464506391 26.64582789879242 29.299924928662318 21.897200721906163 28-29 21.934596358941764 26.69765875076955 29.488008336831907 21.879736553456784 30-31 22.09240541827698 26.597231786826676 29.31204542776875 21.998317367127594 32-33 22.01627208870495 26.67721492730993 29.22670242123237 22.079810562752748 34-35 22.396040957942397 26.659223851704873 29.092997790390108 21.851737399962627 36-37 22.46656426840737 26.439697505760318 29.12567665674884 21.96806156908347 38-39 22.238248299421333 26.622362335609623 29.19880456638646 21.94058479858258 40-41 22.37722931836282 26.545195240071628 29.173537713688386 21.904037727877164 42-43 22.17723527181214 26.67214851778435 28.888547771345408 22.262068439058105 44-45 22.303392219240965 26.887777699047138 28.650770563672832 22.15805951803906 46-47 22.365000466296706 26.975695819226893 28.252552269205534 22.406751445270874 48-49 22.532100847531737 26.999693167390383 28.151501961388874 22.316704023689006 50-51 22.468206081048006 27.20572775919144 28.010575764520144 22.31549039524041 52-53 22.652552905860507 27.271313764567466 27.772569101616757 22.30356422795527 54-55 22.378021562259907 27.205070466474286 27.892029397952633 22.524878573313178 56-57 22.375748182540775 27.50221944917835 27.758215280762393 22.36381708751848 58-59 22.502943384954925 27.141933797692587 27.8893834863451 22.465739331007388 60-61 22.52302827380335 26.99775429837049 28.174669222404447 22.30454820542171 62-63 22.476274775019203 27.03191291466353 28.13299169075738 22.358820619559882 64-65 22.340713634512635 27.010770595573813 28.08902493137491 22.55949083853864 66-67 22.2484341046165 27.22187880894873 27.91232479354409 22.61736229289068 68-69 22.14794001710554 27.17724744188547 28.072218300405005 22.602594240603985 70-71 22.231304681437997 27.083909975460767 27.9018789074764 22.782906435624835 72-73 22.378220690410487 27.084232867096897 27.71437389960954 22.82317254288308 74-75 22.256404044292736 27.32278839149258 27.541845951143905 22.87896161307077 76-77 22.332379345699906 27.08892481314028 27.75275205030384 22.82594379085597 78-79 22.320180124386457 27.133890845391797 27.688083984588268 22.85784504563348 80-81 22.12113860451285 27.37178021371154 27.64604678130062 22.86103440047499 82-83 22.237320929862086 27.286281563886117 27.646089537972905 22.830307968278884 84-85 22.254606424086056 27.09386156384277 27.567024179847593 23.08450783222358 86-87 22.204311844534157 27.049431645860178 27.75957950530153 22.986677004304138 88-89 22.294145421230706 26.944488842052422 27.631763813540267 23.1296019231766 90-91 22.09755715627936 27.08019870594585 27.725776465806184 23.0964676719686 92-93 22.189878647986234 27.083635482645153 27.577705612260985 23.14878025710763 94-95 22.021622249510468 27.07493943115064 27.53036739570542 23.373070923633467 96-97 22.02264784396448 26.958653853653065 27.48078454513066 23.53791375725179 98-99 21.857600140717224 26.899956357746994 27.454395505013878 23.788047996521893 100-101 22.169471455012925 26.2728579436405 28.189093634142143 23.368576967204437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 865.0 1 638.5 2 449.0 3 522.0 4 491.5 5 507.5 6 921.5 7 1861.0 8 3256.0 9 4150.0 10 3776.5 11 3187.0 12 3193.5 13 3398.0 14 3721.0 15 4049.5 16 4204.0 17 4424.0 18 4729.5 19 5135.0 20 5743.0 21 6579.5 22 7698.0 23 9253.5 24 11190.5 25 13722.5 26 17480.5 27 21671.5 28 25467.0 29 29913.0 30 34933.5 31 40386.0 32 45648.5 33 50593.0 34 56787.5 35 62467.0 36 69075.0 37 76724.5 38 80618.5 39 82326.0 40 85343.0 41 90300.0 42 95631.0 43 100060.5 44 104974.5 45 108650.0 46 109602.0 47 113046.5 48 124674.5 49 126905.5 50 118534.5 51 113203.5 52 108195.5 53 105553.0 54 102297.0 55 95406.5 56 86374.0 57 77076.5 58 68544.0 59 60044.5 60 50703.5 61 40947.0 62 32636.0 63 25573.0 64 19148.0 65 14230.5 66 10243.5 67 7424.5 68 5310.5 69 3556.0 70 2494.5 71 1685.0 72 1104.5 73 794.0 74 583.5 75 437.5 76 269.0 77 115.0 78 57.5 79 28.5 80 13.5 81 8.0 82 6.5 83 3.5 84 3.0 85 4.0 86 4.0 87 3.0 88 1.5 89 2.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4020729240475452 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.960766776131837E-4 16-17 0.002455913857162059 18-19 5.14414389000161E-4 20-21 0.0 22-23 0.0 24-25 8.297006274196145E-5 26-27 3.3188025096784576E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012810577687358849 36-37 0.02369624991910419 38-39 0.011184364457616404 40-41 0.006322318780937462 42-43 0.0053930540782274935 44-45 0.001609619217194052 46-47 0.0010620168030971064 48-49 0.0030367042963557887 50-51 6.471664893872993E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.46730564677653E-4 60-61 0.0015100551419036982 62-63 0.0014768671168069137 64-65 7.965126023228298E-4 66-67 0.0027380120704847276 68-69 0.0025222899073556277 70-71 3.3188025096784576E-5 72-73 0.0 74-75 0.0 76-77 0.0925448079823838 78-79 0.0035179306602591653 80-81 2.655042007742766E-4 82-83 0.0 84-85 1.9912815058070746E-4 86-87 0.09212995766867399 88-89 0.4347133467302328 90-91 0.38879771400883134 92-93 0.0 94-95 0.0011615808783874604 96-97 1.6594012548392288E-5 98-99 0.0 100-101 4.6463235135498415E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3013135.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.205669141368524 #Duplication Level Percentage of deduplicated Percentage of total 1 73.63963402094932 38.444063693891465 2 14.266397785902877 14.89573685299996 3 4.984062158938386 7.805889001486569 4 2.2742214911803487 4.749090188910042 5 1.3171792991145979 3.43821133447182 6 0.8224658704596142 2.5762428667969366 7 0.5264185513155317 1.9237422903900026 8 0.3621742373224999 1.5126038724148734 9 0.27277485945155117 1.2816354656349886 >10 1.3853042437564491 13.120285242758978 >50 0.09781451414175467 3.5335472880948284 >100 0.04849440479074578 4.452341790374907 >500 0.002421362118921404 0.8521285737420595 >1k 4.460403902361161E-4 0.4338151439872672 >5k 6.372005574801659E-5 0.20388413599401584 >10k+ 1.2744011149603318E-4 0.776782258051275 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12321 0.4089096572174828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11030 0.3660639168175339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6129 0.2034094058181927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.3188025096784576E-5 0.0 0.0 0.0 0.0 9 3.3188025096784576E-5 0.0 0.0 0.0 0.0 10-11 3.3188025096784576E-5 0.0 0.0 0.0 0.0 12-13 6.637605019356915E-5 0.0 0.0 3.3188025096784576E-5 0.0 14-15 1.327521003871383E-4 0.0 0.0 6.637605019356915E-5 0.0 16-17 1.327521003871383E-4 0.0 0.0 9.956407529035373E-5 0.0 18-19 1.493461129355306E-4 0.0 0.0 1.493461129355306E-4 0.0 20-21 1.659401254839229E-4 0.0 0.0 2.3231617567749205E-4 0.0 22-23 1.9912815058070746E-4 0.0 0.0 2.655042007742766E-4 0.0 24-25 2.3231617567749205E-4 0.0 0.0 4.314443262581995E-4 0.0 26-27 2.3231617567749205E-4 0.0 0.0 7.135425395808684E-4 0.0 28-29 2.986922258710612E-4 0.0 0.0 0.0018087473677747595 0.0 30-31 3.318802509678458E-4 0.0 0.0 0.005144143890001609 1.6594012548392288E-5 32-33 3.318802509678458E-4 0.0 0.0 0.01194768903484245 3.3188025096784576E-5 34-35 3.484742635162381E-4 0.0 0.0 0.023663061894007405 3.3188025096784576E-5 36-37 3.650682760646304E-4 0.0 0.0 0.0438911631904976 3.3188025096784576E-5 38-39 3.982563011614149E-4 0.0 0.0 0.07293068515018411 3.3188025096784576E-5 40-41 3.982563011614149E-4 0.0 0.0 0.10517285153171033 3.3188025096784576E-5 42-43 4.314443262581995E-4 0.0 0.0 0.1387923209547531 3.3188025096784576E-5 44-45 4.978203764517687E-4 0.0 0.0 0.18435947941263833 3.3188025096784576E-5 46-47 6.471664893872993E-4 0.0 0.0 0.23702887524123545 3.3188025096784576E-5 48-49 6.637605019356916E-4 0.0 0.0 0.3004345971886424 3.3188025096784576E-5 50-51 6.637605019356916E-4 0.0 0.0 0.367889258197857 3.3188025096784576E-5 52-53 6.637605019356916E-4 0.0 0.0 0.43416574431613586 3.3188025096784576E-5 54-55 6.637605019356916E-4 0.0 0.0 0.5138004105358704 3.3188025096784576E-5 56-57 6.969485270324761E-4 0.0 0.0 0.6075897694593837 3.3188025096784576E-5 58-59 6.969485270324761E-4 3.3188025096784576E-5 0.0 0.717541696605031 3.3188025096784576E-5 60-61 6.969485270324761E-4 3.3188025096784576E-5 0.0 0.8415985344168118 3.3188025096784576E-5 62-63 6.969485270324761E-4 3.3188025096784576E-5 0.0 0.9670824573077542 3.3188025096784576E-5 64-65 6.969485270324761E-4 3.3188025096784576E-5 0.0 1.1035682105182807 3.3188025096784576E-5 66-67 6.969485270324761E-4 3.3188025096784576E-5 0.0 1.2640986879114278 3.3188025096784576E-5 68-69 6.969485270324761E-4 3.3188025096784576E-5 0.0 1.4482258511483885 3.3188025096784576E-5 70-71 7.135425395808684E-4 3.3188025096784576E-5 0.0 1.6545889912001952 3.3188025096784576E-5 72-73 7.301365521292608E-4 3.3188025096784576E-5 0.0 1.8690666033881655 3.3188025096784576E-5 74-75 7.301365521292608E-4 3.3188025096784576E-5 0.0 2.090447324796267 3.3188025096784576E-5 76-77 7.301365521292608E-4 3.3188025096784576E-5 0.0 2.3343295272199884 3.3188025096784576E-5 78-79 7.301365521292608E-4 3.3188025096784576E-5 0.0 2.6186679322366904 3.3188025096784576E-5 80-81 9.956407529035374E-4 3.3188025096784576E-5 0.0 2.9352816916600153 4.9782037645176864E-5 82-83 0.001028828778000322 3.3188025096784576E-5 0.0 3.2584334920274065 6.637605019356915E-5 84-85 0.001028828778000322 3.3188025096784576E-5 0.0 3.5963705575754155 6.637605019356915E-5 86-87 0.001028828778000322 3.3188025096784576E-5 0.0 3.9567261340763027 6.637605019356915E-5 88-89 0.001028828778000322 3.3188025096784576E-5 0.0 4.353024341757007 6.637605019356915E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11830 0.0 34.479847 1 GTATCAA 17160 0.0 29.629438 1 ATCAACG 20590 0.0 24.545061 3 TATCAAC 20835 0.0 24.324762 2 TCAACGC 20860 0.0 24.204615 4 CAACGCA 21075 0.0 24.00272 5 AACGCAG 21460 0.0 23.572102 6 CTATGCG 150 3.057433E-5 22.145834 9 GAGTACT 11450 0.0 21.863892 12-13 ACGCAGA 23840 0.0 20.940176 7 CGCAGAG 23815 0.0 20.742973 8 GTACTTT 12235 0.0 20.403086 14-15 GTACATG 11685 0.0 20.104555 1 AGTACTT 12000 0.0 19.457829 12-13 GCAGAGT 24605 0.0 19.364023 9 TACATGG 11985 0.0 19.124052 2 ACATGGG 12050 0.0 18.1939 3 AGAGTAC 20845 0.0 18.076965 10-11 GTACCGT 345 1.0986696E-9 17.881136 6 CAGAGTA 23745 0.0 17.478113 10-11 >>END_MODULE