FastQCFastQC Report
Fri 27 May 2016
SRR939016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025222
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126590.4184486295551203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105420.3484702940808972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.19211813215691279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115150.034.4607161
GTATCAA168550.029.3934361
ATCAACG201000.024.5577563
TATCAAC203850.024.33052
TCAACGC203900.024.2781144
CAACGCA203950.024.2025475
AACGCAG207950.023.7789786
GAGTACT103450.023.1702912-13
ACGCAGA227150.021.3944287
GTACTTT110300.021.30281614-15
CGCAGAG225000.021.115858
GTACATG119400.020.5281141
TACATGG117950.020.1421682
GCAGAGT233450.019.724139
AGTACTT108750.019.62590812-13
ACATGGG122050.018.7287943
CAGAGTA222650.017.80086110-11
ACTTTTT135300.017.78751216-17
AGAGTAC199200.017.73451610-11
TAATACC9000.016.8270474
Produced by FastQC (version 0.11.5)