##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939016_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025222 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.649459114074933 33.0 31.0 34.0 30.0 34.0 2 31.735202243008942 33.0 31.0 34.0 30.0 34.0 3 31.76759094043346 34.0 31.0 34.0 30.0 34.0 4 35.30750139989726 37.0 35.0 37.0 33.0 37.0 5 35.34127313631859 37.0 35.0 37.0 33.0 37.0 6 35.41134105199552 37.0 35.0 37.0 33.0 37.0 7 35.32133245097385 37.0 35.0 37.0 33.0 37.0 8 35.30164728406709 37.0 35.0 37.0 33.0 37.0 9 36.87763807085893 39.0 37.0 39.0 33.0 39.0 10-11 36.76251065211082 39.0 37.0 39.0 32.0 39.0 12-13 36.626434853376054 39.0 37.0 39.0 32.0 39.0 14-15 37.80622066744193 40.0 38.0 41.0 32.0 41.0 16-17 37.71829720265157 40.0 38.0 41.0 32.0 41.0 18-19 37.65943276228984 40.0 37.5 41.0 32.0 41.0 20-21 37.539723531033424 40.0 37.0 41.0 32.0 41.0 22-23 37.62897351004323 40.0 37.5 41.0 32.0 41.0 24-25 37.56602209689074 40.0 37.0 41.0 32.0 41.0 26-27 37.47654287850611 40.0 37.0 41.0 32.0 41.0 28-29 37.33829897442237 40.0 37.0 41.0 31.0 41.0 30-31 37.143499386160755 40.0 37.0 41.0 31.0 41.0 32-33 36.94624411034959 40.0 36.0 41.0 30.0 41.0 34-35 36.623096420692434 39.5 36.0 41.0 30.0 41.0 36-37 36.35347472020236 39.0 35.0 41.0 30.0 41.0 38-39 36.146656509836305 39.0 35.0 41.0 29.0 41.0 40-41 35.982038177694065 39.0 35.0 41.0 28.5 41.0 42-43 35.76986531897494 39.0 35.0 41.0 27.5 41.0 44-45 35.66768554506082 39.0 35.0 41.0 27.0 41.0 46-47 35.420169164444786 39.0 35.0 41.0 25.5 41.0 48-49 35.21926407384318 39.0 35.0 41.0 24.5 41.0 50-51 34.06705772336708 38.0 33.5 40.0 23.5 40.5 52-53 34.211317219033845 38.0 33.5 39.5 23.5 40.5 54-55 34.695493752194054 39.0 34.0 40.0 23.5 41.0 56-57 34.587584316126225 38.5 34.0 40.0 23.0 41.0 58-59 34.37777194533162 38.0 34.0 40.0 23.0 41.0 60-61 34.10843303400544 38.0 34.0 40.0 22.0 41.0 62-63 33.74382739514654 37.5 33.5 40.0 21.5 41.0 64-65 33.32699567172261 37.0 33.0 40.0 20.0 41.0 66-67 32.948278837057245 36.0 32.5 39.0 18.5 41.0 68-69 32.5545862419353 36.0 32.0 39.0 17.5 41.0 70-71 32.1423241005123 35.0 32.0 39.0 14.0 40.0 72-73 31.70067419845552 35.0 31.0 38.0 11.5 40.0 74-75 31.22875759200482 35.0 31.0 37.0 7.5 39.0 76-77 30.807552801083688 35.0 31.0 37.0 7.0 39.0 78-79 30.37684474065044 35.0 30.0 36.0 2.0 39.0 80-81 29.93477404302891 34.0 30.0 36.0 2.0 37.5 82-83 29.54566689651206 34.0 29.5 35.0 2.0 37.0 84-85 29.189311891821493 34.0 29.0 35.0 2.0 36.5 86-87 28.78222689111741 34.0 29.0 35.0 2.0 36.0 88-89 28.36418368635426 34.0 29.0 35.0 2.0 36.0 90-91 27.934398368119762 34.0 27.5 35.0 2.0 35.5 92-93 27.502118191656677 34.0 27.0 35.0 2.0 35.0 94-95 27.02009951666357 33.5 25.5 35.0 2.0 35.0 96-97 26.418970905275714 33.0 25.0 35.0 2.0 35.0 98-99 25.67116033137403 33.0 22.0 35.0 2.0 35.0 100-101 22.972500034708197 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2238607513354296 1101 2 0.3543609462430304 1101 3 0.32898455006365523 1101 4 0.15942055874072736 1101 5 -0.007035647084499885 1101 6 0.24865938589085346 1101 7 0.20140276670865376 1101 8 0.2472361851922642 1101 9 0.24599164944307006 1101 10-11 0.3159839283624706 1101 12-13 0.3728307897977885 1101 14-15 0.16575878071252248 1101 16-17 0.4051446980536113 1101 18-19 0.3470810600014289 1101 20-21 0.42916968041455306 1101 22-23 0.42494767858210025 1101 24-25 0.6013237800371698 1101 26-27 0.47347790919776855 1101 28-29 0.4204350875122458 1101 30-31 0.5473421185068759 1101 32-33 0.46645682078250417 1101 34-35 0.387846431402302 1101 36-37 0.4358861847268827 1101 38-39 0.42409955966198964 1101 40-41 0.47122277577276606 1101 42-43 0.24399835536348746 1101 44-45 0.4268624179833793 1101 46-47 0.3171263813011649 1101 48-49 0.4922801961701637 1101 50-51 0.588907247003327 1101 52-53 0.5546236053303915 1101 54-55 0.32160668214655885 1101 56-57 0.4692988145358967 1101 58-59 0.4462941796413631 1101 60-61 0.36039957425669655 1101 62-63 0.4053904421072687 1101 64-65 0.3661471955517186 1101 66-67 0.3686699258513002 1101 68-69 0.23712681163027582 1101 70-71 0.15768108399424818 1101 72-73 0.16497417051727936 1101 74-75 0.1434513197377747 1101 76-77 0.16975056861600635 1101 78-79 -0.005763056462289029 1101 80-81 -0.0849750266946181 1101 82-83 0.01565183356865063 1101 84-85 0.019390977436799517 1101 86-87 -0.023321781237594763 1101 88-89 -0.01628147054499607 1101 90-91 -0.15933737734638598 1101 92-93 0.06575400194349967 1101 94-95 -0.0031824273148011173 1101 96-97 -0.34749425656168853 1101 98-99 -0.1569053329153185 1101 100-101 0.15111569388336932 1102 1 0.36854059810703177 1102 2 0.1779484273355152 1102 3 0.18009619289390955 1102 4 0.19986164775750836 1102 5 0.30281260773396923 1102 6 0.3142683978773917 1102 7 0.43719420500427475 1102 8 0.29751827619179494 1102 9 0.38307980614536064 1102 10-11 0.2747761609718822 1102 12-13 0.4066520920526031 1102 14-15 0.48432291513672254 1102 16-17 0.41927904062720245 1102 18-19 0.5068021453241656 1102 20-21 0.589734578368784 1102 22-23 0.504704617633557 1102 24-25 0.5237414090455275 1102 26-27 0.6410006320289057 1102 28-29 0.5558787045270748 1102 30-31 0.5054929065939362 1102 32-33 0.4978733182552091 1102 34-35 0.39957993376635415 1102 36-37 0.48712612812211376 1102 38-39 0.5134144418745379 1102 40-41 0.47925937250903416 1102 42-43 0.3480240756447017 1102 44-45 0.4765507559366071 1102 46-47 0.6065968496664453 1102 48-49 0.5049191952508423 1102 50-51 0.4011626387054221 1102 52-53 0.3900905200393723 1102 54-55 0.4300657857147243 1102 56-57 0.46382962836003827 1102 58-59 0.5009478532376406 1102 60-61 0.3258811485558155 1102 62-63 0.3584842429616586 1102 64-65 0.45778951507853805 1102 66-67 0.2866587191706671 1102 68-69 0.19736902835806802 1102 70-71 0.23060653976850176 1102 72-73 0.16446530596620335 1102 74-75 0.2079659930873099 1102 76-77 0.0759350219877355 1102 78-79 0.10293823584844475 1102 80-81 0.01968444217392573 1102 82-83 0.0387997485066478 1102 84-85 0.05158458943760991 1102 86-87 0.09438299709848863 1102 88-89 -0.027413523927730665 1102 90-91 -0.13173269980373092 1102 92-93 -0.1591405754074131 1102 94-95 -0.024335229404737646 1102 96-97 -0.23277789141242877 1102 98-99 -0.3058950781985246 1102 100-101 0.11981795868043577 1103 1 0.09060721986681486 1103 2 0.25909239408023055 1103 3 0.3276996928216356 1103 4 0.3564283429640369 1103 5 0.30478671585343875 1103 6 0.28084503619341916 1103 7 0.32531204284907034 1103 8 0.29722343578192323 1103 9 0.40147613884099087 1103 10-11 0.45356895394392893 1103 12-13 0.458453468637785 1103 14-15 0.4649137781272259 1103 16-17 0.5176161594429729 1103 18-19 0.49563683513016343 1103 20-21 0.5285234151654308 1103 22-23 0.5318569145854894 1103 24-25 0.5972875292137729 1103 26-27 0.4782692674585647 1103 28-29 0.4092029160279651 1103 30-31 0.5952507430107659 1103 32-33 0.6155465955193122 1103 34-35 0.5204303827278878 1103 36-37 0.5775913357247617 1103 38-39 0.5264207196694457 1103 40-41 0.5113812344162696 1103 42-43 0.4698801305536051 1103 44-45 0.5931967736305452 1103 46-47 0.5218264278129041 1103 48-49 0.4005434077466248 1103 50-51 0.26696887623630516 1103 52-53 0.3275917270825346 1103 54-55 0.5223535260772465 1103 56-57 0.5046300867145632 1103 58-59 0.49003805579264537 1103 60-61 0.4761407871978207 1103 62-63 0.39202675809431753 1103 64-65 0.26877410530713775 1103 66-67 0.28830702805199593 1103 68-69 0.2342724783654191 1103 70-71 0.18117559112329218 1103 72-73 0.26235747209513605 1103 74-75 0.187346477086745 1103 76-77 0.24554439648479942 1103 78-79 0.2641289158950997 1103 80-81 0.3252716035653975 1103 82-83 0.31963313207248234 1103 84-85 0.21811048804832467 1103 86-87 0.25059398765809604 1103 88-89 0.29913516365789405 1103 90-91 0.3544107958283824 1103 92-93 0.3541473165304758 1103 94-95 0.2908804201951689 1103 96-97 0.32846183116369687 1103 98-99 0.348436729042092 1103 100-101 0.7111089886379318 1104 1 0.06036156191031594 1104 2 0.10886898963995861 1104 3 0.047428246285129205 1104 4 0.19408198784660158 1104 5 0.1502470564945 1104 6 0.04198773107105325 1104 7 -0.0020452941468178665 1104 8 0.07198365993973965 1104 9 0.1378235486823769 1104 10-11 0.1133526775830731 1104 12-13 0.13843747392299122 1104 14-15 0.1786469213784443 1104 16-17 0.13730269780420912 1104 18-19 0.22276381346195961 1104 20-21 0.23014548787184452 1104 22-23 0.18984355088537797 1104 24-25 0.21322052526724633 1104 26-27 0.19785439005573835 1104 28-29 0.09264172175592478 1104 30-31 0.13349375836222066 1104 32-33 0.18091311209064997 1104 34-35 0.12396689530054772 1104 36-37 0.11435918634803954 1104 38-39 0.1321460741899898 1104 40-41 0.20782646700607899 1104 42-43 0.0564999141613427 1104 44-45 0.17270354530599263 1104 46-47 0.25029759965757137 1104 48-49 0.29758322647318636 1104 50-51 0.2683217625289487 1104 52-53 0.1837325964764176 1104 54-55 0.20955717472668312 1104 56-57 0.15462429152804447 1104 58-59 0.15622372197831425 1104 60-61 0.15716039231381274 1104 62-63 0.23714315441553424 1104 64-65 0.16398670172455354 1104 66-67 0.19329581436607413 1104 68-69 0.3008629383293666 1104 70-71 0.19743978208627766 1104 72-73 0.16090259051977185 1104 74-75 0.07235143197122085 1104 76-77 0.10177451175106 1104 78-79 0.12512296423854252 1104 80-81 0.026828190664151208 1104 82-83 0.06675234485435055 1104 84-85 0.0922367188010611 1104 86-87 0.16107776982862632 1104 88-89 0.19481717018327416 1104 90-91 0.2750465857412827 1104 92-93 0.24713572036225528 1104 94-95 0.0322739886971668 1104 96-97 0.24134163320012547 1104 98-99 0.20570534173239352 1104 100-101 0.4451423805026522 1105 1 0.13653939867209175 1105 2 0.14364743098774468 1105 3 0.18290220730653317 1105 4 0.14140927531987302 1105 5 0.17735512009891607 1105 6 0.21620680747990662 1105 7 0.23787704358574047 1105 8 0.18507565040398788 1105 9 0.27751486574595674 1105 10-11 0.12187217916826398 1105 12-13 0.30677781160639483 1105 14-15 0.29933433075198934 1105 16-17 0.3542458519736371 1105 18-19 0.3099409375004001 1105 20-21 0.2944343620147336 1105 22-23 0.27198000054007565 1105 24-25 0.33381700097321243 1105 26-27 0.3043414012872745 1105 28-29 0.43718078636797486 1105 30-31 0.3474987720022682 1105 32-33 0.29525974140078404 1105 34-35 0.1793700755212413 1105 36-37 0.1803954615083967 1105 38-39 0.2563577775987653 1105 40-41 0.22025120096366635 1105 42-43 0.1034487209641668 1105 44-45 0.11211223070007748 1105 46-47 0.18089677879689248 1105 48-49 0.20221101285279985 1105 50-51 0.07307611874315967 1105 52-53 0.11303904293615119 1105 54-55 0.12370841953485723 1105 56-57 0.054689419695357344 1105 58-59 0.029302441042439398 1105 60-61 0.14572364036611418 1105 62-63 0.17781630932226733 1105 64-65 0.2958282313762979 1105 66-67 0.27177402715086174 1105 68-69 0.1060596745479998 1105 70-71 -0.15616192844974108 1105 72-73 0.08312727040868495 1105 74-75 0.07005105594821615 1105 76-77 0.02505475948386149 1105 78-79 0.01367515279073217 1105 80-81 -0.05976277986842504 1105 82-83 -0.0064099348772757025 1105 84-85 -0.009397629548615782 1105 86-87 -0.009630529711792235 1105 88-89 0.08286307939706816 1105 90-91 0.1203131196939431 1105 92-93 0.19733110486420813 1105 94-95 0.08334553137455103 1105 96-97 0.029158979411633368 1105 98-99 0.1438176079468505 1105 100-101 0.31473121286520467 1106 1 0.19957548330245345 1106 2 0.26363446618761444 1106 3 0.23587042383621437 1106 4 0.1960521848663035 1106 5 0.19631833028854828 1106 6 0.18208507413869057 1106 7 0.338125389588221 1106 8 0.2910022284632561 1106 9 0.4408971893797755 1106 10-11 0.2949358358989045 1106 12-13 0.21807043721932473 1106 14-15 0.32418480850049036 1106 16-17 0.3904830158360113 1106 18-19 0.3648213525492068 1106 20-21 0.40746321964501675 1106 22-23 0.3942206552624796 1106 24-25 0.46136533974869565 1106 26-27 0.40378831678174265 1106 28-29 0.32260305122220245 1106 30-31 0.3479302020065802 1106 32-33 0.4702803821509889 1106 34-35 0.48906673861820593 1106 36-37 0.361013851814576 1106 38-39 0.3391551084267377 1106 40-41 0.348562207246772 1106 42-43 0.529768907727366 1106 44-45 0.5669179667481288 1106 46-47 0.538023038868161 1106 48-49 0.5622013549527054 1106 50-51 0.42702096578261006 1106 52-53 0.3412616827183683 1106 54-55 0.29703925626817096 1106 56-57 0.46326348878110224 1106 58-59 0.6146962358963677 1106 60-61 0.5680348130892199 1106 62-63 0.5500844485449505 1106 64-65 0.4008770574267899 1106 66-67 0.582554740258665 1106 68-69 0.5724634317120447 1106 70-71 0.6128363217402466 1106 72-73 0.6309529288869449 1106 74-75 0.7004492417521995 1106 76-77 0.649164250724958 1106 78-79 0.7630450897844341 1106 80-81 0.6250196764794005 1106 82-83 0.7298286524851108 1106 84-85 0.686449473409855 1106 86-87 0.8507950748924635 1106 88-89 1.0291272678597103 1106 90-91 0.9333574168243892 1106 92-93 0.9607070774979682 1106 94-95 0.9670157962112533 1106 96-97 0.8505442201254851 1106 98-99 0.872838721737061 1106 100-101 1.3049266922671592 1107 1 0.018507076960986524 1107 2 -0.05664471647274638 1107 3 0.11259348715752182 1107 4 0.12349928897771179 1107 5 0.056491419144855115 1107 6 -0.011133049670362993 1107 7 -0.0355999197147554 1107 8 0.14099042195814349 1107 9 0.0620939846000681 1107 10-11 0.09332713093523637 1107 12-13 0.11463035111420083 1107 14-15 0.036685151712845254 1107 16-17 0.0677090911889664 1107 18-19 0.11613709144422302 1107 20-21 0.14398101451934764 1107 22-23 0.07497495365405626 1107 24-25 0.24395129385159464 1107 26-27 0.3146280613219119 1107 28-29 0.22899723473372546 1107 30-31 0.233651744210718 1107 32-33 0.1708459961167179 1107 34-35 -0.02556811089004185 1107 36-37 -0.03385086760245315 1107 38-39 0.09196752777184258 1107 40-41 0.07960974627687989 1107 42-43 0.0765924087130685 1107 44-45 0.24890398564952676 1107 46-47 0.29349384385214705 1107 48-49 0.028949019735826198 1107 50-51 0.1149110609485362 1107 52-53 0.19452549495650118 1107 54-55 0.2614011833094594 1107 56-57 0.3386158992301347 1107 58-59 0.292724055317251 1107 60-61 0.14231420442269638 1107 62-63 -0.007463477628697035 1107 64-65 0.09766513889717032 1107 66-67 0.20003962518642737 1107 68-69 0.25742551361074817 1107 70-71 0.31879476284225916 1107 72-73 0.15978688549391862 1107 74-75 0.1865348636255355 1107 76-77 0.2566174502878411 1107 78-79 0.24025963320217159 1107 80-81 0.17991031529370005 1107 82-83 0.18083577482796187 1107 84-85 0.22238792582414746 1107 86-87 0.33604737716096267 1107 88-89 0.25604060811220464 1107 90-91 0.18548121489471114 1107 92-93 0.17311598893819635 1107 94-95 0.2944585072044852 1107 96-97 0.22843777487197414 1107 98-99 0.2696515144972018 1107 100-101 0.3737759149598112 1108 1 0.2507191252740135 1108 2 0.1518943351192945 1108 3 0.1558168356553118 1108 4 0.28324973897753836 1108 5 0.2835948493514948 1108 6 0.2202777891157126 1108 7 0.2362538527412852 1108 8 0.20525708953218214 1108 9 0.34696196763674436 1108 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2316 94-95 -2.985867664723674 2316 96-97 -3.124567884993656 2316 98-99 -3.4742225326117264 2316 100-101 -4.005814737205739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11715.0 3 3325.0 4 3461.0 5 3695.0 6 4273.0 7 5373.0 8 6468.0 9 7698.0 10 9473.0 11 11566.0 12 14402.0 13 19985.0 14 19457.0 15 18822.0 16 22867.0 17 29742.0 18 26364.0 19 19425.0 20 19595.0 21 21070.0 22 22226.0 23 24879.0 24 28051.0 25 32195.0 26 37142.0 27 43142.0 28 50587.0 29 59565.0 30 71081.0 31 85461.0 32 106020.0 33 134474.0 34 178975.0 35 254329.0 36 389488.0 37 597740.0 38 545486.0 39 85583.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.891726599743194 19.39726437954554 15.310476709563863 22.4005323111474 2 15.224325623606475 20.563686719985284 39.96672907721825 24.24525857918999 3 18.82744265409618 24.93861497652298 30.41719316669959 25.816749202681255 4 12.967586659002544 17.460230790638786 37.53959878525669 32.032583765101975 5 13.980608291661248 37.06709263695531 34.102939069239035 14.849360002144413 6 30.945672779485278 36.35391267811593 17.845438213993866 14.85497632840492 7 27.65279014419156 33.38494966910951 20.98395956190966 17.978300624789277 8 26.065400149351063 34.65424434614905 20.05463191778479 19.2257235867151 9 26.264975169186695 17.240085459423817 20.7879353581583 35.707004013231185 10-11 24.698209804980305 26.909402394867232 27.186576912082327 21.205810888070133 12-13 25.860516147561107 24.37883687796834 27.650965452299133 22.109681522171424 14-15 23.02833036295134 25.47140061507754 26.41769284375085 25.082576178220272 16-17 21.984113303222635 28.726198988023764 26.942469286855523 22.34721842189807 18-19 21.8464604624283 27.287253746895544 29.44675145517052 21.41953433550563 20-21 22.83023029220467 26.41438632618009 29.306651520007303 21.448731861607932 22-23 22.535950779662343 26.31473130181346 29.146330424992218 22.00298749353198 24-25 22.313171211734797 26.52311514567956 29.254740343016287 21.908973299569357 26-27 22.217437314490578 26.586762385417366 29.205020865103226 21.990779434988827 28-29 21.975376513027484 26.63898140661289 29.408974750632417 21.976667329727213 30-31 22.133140984049273 26.59518435100236 29.2481615204463 22.02351314450207 32-33 22.035368988025734 26.653041105192766 29.111433475363707 22.200156431417795 34-35 22.452499560714994 26.679060117584026 28.947894576244693 21.920545745456284 36-37 22.473767256414988 26.427225855151693 29.049236586010647 22.049770302422672 38-39 22.287287751555404 26.589592108618536 29.090292801531515 22.03282733829454 40-41 22.44356223068985 26.495465868132122 29.095633041914752 21.965338859263273 42-43 22.23561032692167 26.61610805635526 28.817393934961405 22.330887681761666 44-45 22.318926696165143 26.833360654947302 28.60488191243761 22.242830736449946 46-47 22.36930627215854 26.93826919550751 28.224239654609782 22.46818487772417 48-49 22.54045220808183 26.91872179768525 28.163140362353083 22.377685631879835 50-51 22.470676235950787 27.151671557216606 28.045354509589153 22.332297697243455 52-53 22.639686781314886 27.232271713351963 27.829915124493386 22.298126380839765 54-55 22.38455009970815 27.10089932677257 27.995670862527444 22.518879710991836 56-57 22.344185972299876 27.44787695097864 27.863867446803276 22.34406962991821 58-59 22.454036324208904 27.066441705331894 27.9553181850061 22.524203785453096 60-61 22.492287263773836 26.943989082157398 28.232858606729273 22.3308650473395 62-63 22.486935333537357 26.93649384364424 28.21183336206394 22.36473746075447 64-65 22.38225996154416 26.942085598249715 28.123431173508408 22.55222326669772 66-67 22.244480179136914 27.135420902400213 28.014956681802634 22.605142236660235 68-69 22.157921320910077 27.065946879465024 28.18679053330923 22.589341266315664 70-71 22.227305648340558 26.975763366237015 28.005148839889536 22.791782145532892 72-73 22.280715203775806 26.982427882045023 27.926827990668247 22.810028923510924 74-75 22.2379291078282 27.190969196692848 27.746622677779182 22.824479017699762 76-77 22.357989663511297 26.986581851041176 27.864526657150957 22.790901828296576 78-79 22.304493285512137 27.043339775575916 27.847243219397004 22.804923719514942 80-81 22.168115697284104 27.258169901506253 27.760420363208677 22.813294038000965 82-83 22.30585411224143 27.150825822892973 27.792815743955423 22.75050432091018 84-85 22.233281767074015 26.925767030789295 27.77369256257154 23.067258639565146 86-87 22.26287109924689 26.889656058634127 27.966628725621412 22.880844116497574 88-89 22.332518827650496 26.770818318015007 27.869893898123777 23.02676895621072 90-91 22.129654679759298 26.90200830846442 28.0215042999787 22.946832711797587 92-93 22.21632609756333 26.85374547300131 27.920319501590292 23.009608927845072 94-95 22.131133501368357 26.794795448653343 27.849817466872896 23.224253583105416 96-97 22.127796923359362 26.689411883652514 27.814915993356358 23.367875199631765 98-99 22.009346827913834 26.568698420205482 27.81631685490532 23.60563789697536 100-101 22.290509926600414 26.13452799685041 28.290611676499168 23.28435040005001 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3401.0 1 1991.5 2 631.5 3 702.0 4 662.0 5 649.0 6 1073.5 7 2196.0 8 3855.5 9 4922.0 10 4506.0 11 3933.0 12 4093.0 13 4438.0 14 4721.0 15 4982.0 16 5297.0 17 5554.0 18 5809.5 19 6104.5 20 6430.0 21 7083.5 22 8185.5 23 9650.0 24 11245.5 25 13652.5 26 17460.0 27 21519.0 28 25308.0 29 29787.0 30 34646.5 31 40162.5 32 45418.0 33 50486.0 34 56860.0 35 62405.0 36 68764.5 37 75821.0 38 80195.0 39 82605.5 40 85076.0 41 89941.0 42 95111.5 43 99754.5 44 104831.5 45 108098.0 46 109227.0 47 112437.0 48 123952.5 49 125869.0 50 117377.5 51 112926.5 52 108205.5 53 105319.5 54 101971.5 55 94657.0 56 85906.5 57 77003.5 58 68330.0 59 59926.0 60 50924.5 61 41436.5 62 32960.5 63 25988.5 64 19776.0 65 14752.5 66 10788.5 67 8023.5 68 5782.0 69 3987.0 70 2853.5 71 1977.5 72 1382.0 73 997.5 74 712.0 75 495.0 76 316.5 77 177.0 78 114.0 79 81.5 80 51.5 81 35.0 82 28.0 83 23.0 84 21.0 85 19.5 86 13.0 87 13.5 88 14.5 89 11.5 90 9.5 91 7.5 92 5.5 93 3.5 94 3.5 95 2.5 96 1.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29554855808929065 2 0.09133214025284757 3 0.09602601065310248 4 0.1107026195102376 5 0.11288427758359552 6 0.08634077102440747 7 0.09662100830947283 8 0.09298491152054296 9 0.09814155787575259 10-11 0.09116686312607802 12-13 0.09612517692916421 14-15 0.10458736581976462 16-17 0.0931832440726664 18-19 0.10424028385354861 20-21 0.10622360937478308 22-23 0.11984244462059314 24-25 0.1339901666720657 26-27 0.13714695979336392 28-29 0.1282219949478088 30-31 0.12017299887413223 32-33 0.11405774518365924 34-35 0.3901366577394981 36-37 0.5952290443478198 38-39 0.5779741123130798 40-41 0.5413487010209499 42-43 0.5672972099237675 44-45 0.5895435111869476 46-47 0.5782550834285881 48-49 0.6001212473001981 50-51 0.5914111427194434 52-53 0.594799323818219 54-55 0.5848992239247236 56-57 0.5573144714668874 58-59 0.5521908805370316 60-61 0.49925261683274813 62-63 0.5750156517439051 64-65 0.6081041325231669 66-67 0.6216568569182691 68-69 0.6237724041409192 70-71 0.6187810349124792 72-73 0.6114757859092654 74-75 0.6082859373626134 76-77 0.5921714175025833 78-79 0.5689334534787861 80-81 0.5639751396756998 82-83 0.5772964760933247 84-85 0.5872131036994971 86-87 0.5779906400257568 88-89 0.5909153113391348 90-91 0.6045341465849449 92-93 0.6128145306360988 94-95 0.6117732847374506 96-97 0.6094098218246463 98-99 0.6126327257966523 100-101 0.5899732317165485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025222.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.38805912329545 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33400002670211 40.972700474466166 2 13.650264646305835 14.848228012638229 3 4.598890113379809 7.503741221641201 4 2.0743846869884397 4.51287028001544 5 1.1492523514991364 3.125280242046068 6 0.7110745029699448 2.3204377265158374 7 0.4760604606615108 1.8124403136505065 8 0.3364901274437058 1.4640835956650795 9 0.2567689016920954 1.256864598562799 >10 1.275201133898336 12.497503565212313 >50 0.09138580037600469 3.4121413485706187 >100 0.04342583173066858 4.106038334292251 >500 0.0021924127976375805 0.7915288926490122 >1k 4.263024882868294E-4 0.41515768246395796 >5k 6.090035546954707E-5 0.19250809402270017 >10k+ 1.2180071093909414E-4 0.7684756175878642 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12659 0.4184486295551203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10542 0.3484702940808972 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5812 0.19211813215691279 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.305542535390791E-5 2 0.0 0.0 0.0 0.0 3.305542535390791E-5 3 0.0 0.0 0.0 0.0 3.305542535390791E-5 4 0.0 0.0 0.0 0.0 3.305542535390791E-5 5 0.0 0.0 0.0 0.0 3.305542535390791E-5 6 0.0 0.0 0.0 0.0 3.305542535390791E-5 7 0.0 0.0 0.0 0.0 3.305542535390791E-5 8 3.305542535390791E-5 0.0 0.0 0.0 3.305542535390791E-5 9 3.305542535390791E-5 0.0 0.0 3.305542535390791E-5 3.305542535390791E-5 10-11 6.611085070781583E-5 0.0 0.0 6.611085070781583E-5 3.305542535390791E-5 12-13 6.611085070781583E-5 0.0 0.0 9.916627606172374E-5 3.305542535390791E-5 14-15 6.611085070781583E-5 0.0 0.0 9.916627606172374E-5 3.305542535390791E-5 16-17 8.263856338476978E-5 0.0 0.0 9.916627606172374E-5 4.958313803086187E-5 18-19 1.3222170141563165E-4 0.0 0.0 9.916627606172374E-5 6.611085070781583E-5 20-21 1.3222170141563165E-4 0.0 0.0 1.3222170141563165E-4 6.611085070781583E-5 22-23 1.6527712676953955E-4 0.0 0.0 3.47081966216033E-4 9.916627606172374E-5 24-25 2.1486026480040143E-4 0.0 0.0 4.462482422777568E-4 1.3222170141563165E-4 26-27 2.3138797747735538E-4 0.0 0.0 7.933302084937898E-4 1.3222170141563165E-4 28-29 3.140265408621252E-4 0.0 0.0 0.0018511038198188432 1.3222170141563165E-4 30-31 3.63609678892987E-4 0.0 0.0 0.005239284918594404 1.3222170141563165E-4 32-33 3.80137391569941E-4 0.0 0.0 0.012164396530238111 1.3222170141563165E-4 34-35 4.2972052960080285E-4 0.0 0.0 0.023733795404105883 1.9833255212344748E-4 36-37 4.6277595495471075E-4 0.0 0.0 0.0449057953432839 1.9833255212344748E-4 38-39 5.288868056625266E-4 0.0 0.0 0.07500276012801704 1.9833255212344748E-4 40-41 5.288868056625266E-4 0.0 0.0 0.10895068196648047 1.9833255212344748E-4 42-43 5.288868056625266E-4 0.0 0.0 0.14413818225571545 1.9833255212344748E-4 44-45 6.280530817242504E-4 0.0 0.0 0.18849856308065988 1.9833255212344748E-4 46-47 6.941639324320662E-4 0.0 0.0 0.2454200055400893 1.9833255212344748E-4 48-49 8.263856338476978E-4 0.0 0.0 0.30880378365620775 1.9833255212344748E-4 50-51 8.263856338476978E-4 0.0 0.0 0.377245041851474 1.9833255212344748E-4 52-53 8.263856338476978E-4 0.0 0.0 0.4480001798215139 1.9833255212344748E-4 54-55 8.263856338476978E-4 0.0 0.0 0.5279612537526172 1.9833255212344748E-4 56-57 9.255519099094215E-4 0.0 0.0 0.6238715704169809 1.9833255212344748E-4 58-59 9.255519099094215E-4 0.0 0.0 0.7350700213075272 1.9833255212344748E-4 60-61 9.255519099094215E-4 0.0 0.0 0.8602178616974225 1.9833255212344748E-4 62-63 9.255519099094215E-4 0.0 0.0 0.9883241626564926 1.9833255212344748E-4 64-65 9.255519099094215E-4 0.0 0.0 1.1232564089511448 1.9833255212344748E-4 66-67 9.255519099094215E-4 0.0 0.0 1.280005235979376 2.1486026480040143E-4 68-69 9.586073352633295E-4 0.0 0.0 1.4692971292685297 2.3138797747735538E-4 70-71 9.586073352633295E-4 0.0 0.0 1.6768852004910713 2.3138797747735538E-4 72-73 9.586073352633295E-4 0.0 0.0 1.8966376682438513 2.644434028312633E-4 74-75 9.586073352633295E-4 0.0 0.0 2.1220756691575033 2.644434028312633E-4 76-77 9.586073352633295E-4 0.0 0.0 2.3703549689907053 2.644434028312633E-4 78-79 9.586073352633295E-4 0.0 0.0 2.6496567855185504 2.644434028312633E-4 80-81 0.001090829036678961 0.0 0.0 2.9605761163974083 2.644434028312633E-4 82-83 0.001090829036678961 0.0 0.0 3.2859571958686007 2.974988281851712E-4 84-85 0.001123884462032869 0.0 0.0 3.626560298715268 2.974988281851712E-4 86-87 0.001156939887386777 0.0 0.0 3.9867322133714485 2.974988281851712E-4 88-89 0.001156939887386777 0.0 0.0 4.388487853122845 2.974988281851712E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11515 0.0 34.460716 1 GTATCAA 16855 0.0 29.393436 1 ATCAACG 20100 0.0 24.557756 3 TATCAAC 20385 0.0 24.3305 2 TCAACGC 20390 0.0 24.278114 4 CAACGCA 20395 0.0 24.202547 5 AACGCAG 20795 0.0 23.778978 6 GAGTACT 10345 0.0 23.17029 12-13 ACGCAGA 22715 0.0 21.394428 7 GTACTTT 11030 0.0 21.302816 14-15 CGCAGAG 22500 0.0 21.11585 8 GTACATG 11940 0.0 20.528114 1 TACATGG 11795 0.0 20.142168 2 GCAGAGT 23345 0.0 19.72413 9 AGTACTT 10875 0.0 19.625908 12-13 ACATGGG 12205 0.0 18.728794 3 CAGAGTA 22265 0.0 17.800861 10-11 ACTTTTT 13530 0.0 17.787512 16-17 AGAGTAC 19920 0.0 17.734516 10-11 TAATACC 900 0.0 16.827047 4 >>END_MODULE