FastQCFastQC Report
Fri 27 May 2016
SRR939016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025222
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121790.40258202538524446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109060.3605024689097197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62380.20619974335767755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122300.032.4530261
GTATCAA177450.029.4018541
TATCAAC214600.024.2340762
ATCAACG214550.024.1291433
TCAACGC218400.023.7037894
CAACGCA220050.023.5476135
AACGCAG225200.023.0091136
GAGTACT117100.021.88321912-13
GTACTTT122250.020.70903814-15
ACGCAGA247350.020.660917
CGCAGAG247650.020.290998
AGTACTT121550.019.93035912-13
GTACATG117850.019.1235351
GTATAGC11800.018.978091
GCAGAGT256350.018.9730079
TACATGG119100.018.6457442
AGAGTAC216350.017.71168910-11
ACATGGG120750.017.5264243
ACTTTTT146150.017.51671216-17
TACTTTT142750.016.97047414-15
Produced by FastQC (version 0.11.5)