FastQCFastQC Report
Fri 27 May 2016
SRR939015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635736
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93960.2584346058129633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75520.2077158517560131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46390.1275945228146378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106550.032.6067051
GTATCAA157000.029.323881
ATCAACG182350.024.9344023
CAACGCA183750.024.7444295
TATCAAC185200.024.6274152
TCAACGC185450.024.5942154
AACGCAG187700.024.2628086
GTACATG121950.023.2349571
TACATGG122650.022.4744432
ACGCAGA209750.021.3510577
CGCAGAG210300.021.2276868
GAGTACT97300.021.140412-13
ACATGGG126350.020.9166683
GTACTTT100350.020.38034614-15
GCAGAGT219500.019.2811649
AGTACTT99300.018.30704512-13
CAGAGTA210150.017.21017510-11
AGTACAT95200.017.1638892
AGAGTAC183100.016.9087210-11
CATGGGG70800.016.5902124
Produced by FastQC (version 0.11.5)