##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939015_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3635736 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735286610468968 33.0 31.0 34.0 30.0 34.0 2 31.823698420347352 33.0 31.0 34.0 30.0 34.0 3 31.87512157098315 34.0 31.0 34.0 30.0 34.0 4 35.40294757375123 37.0 35.0 37.0 33.0 37.0 5 35.434556304418145 37.0 35.0 37.0 33.0 37.0 6 35.50786718287576 37.0 35.0 37.0 33.0 37.0 7 35.427948563922136 37.0 35.0 37.0 33.0 37.0 8 35.41039943494247 37.0 35.0 37.0 33.0 37.0 9 37.01132865532591 39.0 37.0 39.0 33.0 39.0 10-11 36.888604398119114 39.0 37.0 39.0 33.0 39.0 12-13 36.77503262063032 39.0 37.0 39.0 32.0 39.0 14-15 37.98773563317029 40.0 38.0 41.0 33.0 41.0 16-17 37.89220078135486 40.0 38.0 41.0 33.0 41.0 18-19 37.778098849861486 40.0 38.0 41.0 32.0 41.0 20-21 37.891487170685664 40.0 38.0 41.0 32.0 41.0 22-23 37.82714806575615 40.0 38.0 41.0 32.0 41.0 24-25 37.74771201759424 40.0 38.0 41.0 32.0 41.0 26-27 37.631702356826786 40.0 37.5 41.0 32.0 41.0 28-29 37.49580635117621 40.0 37.0 41.0 32.0 41.0 30-31 37.30339730387465 40.0 37.0 41.0 31.0 41.0 32-33 37.11456057315493 40.0 36.5 41.0 30.5 41.0 34-35 36.802520590053845 40.0 36.0 41.0 30.0 41.0 36-37 36.54357302070338 39.5 36.0 41.0 30.0 41.0 38-39 36.30749936188986 39.0 35.5 41.0 30.0 41.0 40-41 36.15537005437138 39.0 35.0 41.0 29.5 41.0 42-43 35.95428766005013 39.0 35.0 41.0 28.5 41.0 44-45 35.915806593217994 39.0 35.0 41.0 28.0 41.0 46-47 35.73410266862061 39.0 35.0 41.0 27.0 41.0 48-49 35.514816944904695 39.0 35.0 41.0 26.0 41.0 50-51 34.41990975142309 38.0 34.0 40.0 25.0 40.5 52-53 34.51900275487549 38.0 34.0 39.5 24.5 40.5 54-55 35.0007988753859 39.0 35.0 40.0 26.0 41.0 56-57 34.90240229213562 38.5 34.5 40.0 25.0 41.0 58-59 34.67850264705688 38.0 34.0 40.0 25.0 41.0 60-61 34.36130524878594 38.0 34.0 40.0 23.5 41.0 62-63 33.97206851652595 37.5 34.0 40.0 22.5 41.0 64-65 33.57115629407636 37.0 33.0 40.0 22.0 41.0 66-67 33.162682604017455 36.0 33.0 39.0 21.5 41.0 68-69 32.717833885628664 36.0 32.0 39.0 20.0 40.5 70-71 32.27359439739299 35.0 32.0 38.5 18.5 40.0 72-73 31.829360685154256 35.0 31.0 37.5 17.0 40.0 74-75 31.3536189096238 35.0 31.0 37.0 12.5 39.0 76-77 30.920249985147436 35.0 31.0 36.5 8.0 39.0 78-79 30.518720831215468 35.0 30.5 36.0 7.0 38.5 80-81 30.07089843707024 34.0 30.0 36.0 3.5 37.5 82-83 29.60409941755947 34.0 29.5 35.0 2.0 37.0 84-85 29.1933193169141 34.0 29.0 35.0 2.0 36.5 86-87 28.733579940897798 34.0 29.0 35.0 2.0 36.0 88-89 28.26053376812838 34.0 28.5 35.0 2.0 36.0 90-91 27.806905534395234 34.0 27.0 35.0 2.0 35.0 92-93 27.294554115040256 33.5 26.0 35.0 2.0 35.0 94-95 26.75379139189424 33.0 25.0 35.0 2.0 35.0 96-97 26.097072092143105 33.0 24.5 35.0 2.0 35.0 98-99 25.215025788451086 32.5 19.5 35.0 2.0 35.0 100-101 22.009149041624582 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21479670289392772 1101 2 0.36055210328607856 1101 3 0.43228167953591523 1101 4 0.3788221180476228 1101 5 0.21805255015340208 1101 6 0.35008188363496373 1101 7 0.43463679548165857 1101 8 0.3408433766058039 1101 9 0.47792448390961795 1101 10-11 0.42125785993330567 1101 12-13 0.4642564813122121 1101 14-15 0.5801771401929372 1101 16-17 0.5743204965642192 1101 18-19 0.6387660893965474 1101 20-21 0.5017819947980442 1101 22-23 0.6412158938485462 1101 24-25 0.6253987142454562 1101 26-27 0.673829308335776 1101 28-29 0.6083590410114823 1101 30-31 0.5182668843880052 1101 32-33 0.5959901726751866 1101 34-35 0.5083636306455332 1101 36-37 0.3897680041504472 1101 38-39 0.5673826106320021 1101 40-41 0.5123369168093319 1101 42-43 0.4407584371077107 1101 44-45 0.5714816190657004 1101 46-47 0.4595662874550257 1101 48-49 0.5095105852897106 1101 50-51 0.42282829968969793 1101 52-53 0.6046788992673484 1101 54-55 0.5995429529271306 1101 56-57 0.5413847707934707 1101 58-59 0.5730853780478711 1101 60-61 0.6508922903658387 1101 62-63 0.5410837086408904 1101 64-65 0.4741345326765938 1101 66-67 0.5590677579991308 1101 68-69 0.5198911311513115 1101 70-71 0.44849913505345995 1101 72-73 0.37456719972999153 1101 74-75 0.27181503737175916 1101 76-77 0.17304849102141873 1101 78-79 0.167950948605597 1101 80-81 0.1876511455728327 1101 82-83 0.1950246662702142 1101 84-85 0.0637915358705925 1101 86-87 0.084453569196004 1101 88-89 0.05236191159901438 1101 90-91 0.0487786049604928 1101 92-93 -0.021855929967859566 1101 94-95 -0.15430072719585297 1101 96-97 -0.28001983992752244 1101 98-99 -0.3174596029690342 1101 100-101 0.2168885890788843 1102 1 0.21032621973226284 1102 2 0.2798863015461528 1102 3 0.37829749230123966 1102 4 0.20534889171372583 1102 5 0.2869622709406272 1102 6 0.29626550420626074 1102 7 0.3592066666302287 1102 8 0.3151205895539064 1102 9 0.43066318357941213 1102 10-11 0.4205395627803554 1102 12-13 0.369972677400483 1102 14-15 0.452654562415411 1102 16-17 0.6344886094752908 1102 18-19 0.6314396664626329 1102 20-21 0.5135255075008232 1102 22-23 0.4437718078255486 1102 24-25 0.48812308784388136 1102 26-27 0.549649104941615 1102 28-29 0.5999346730991135 1102 30-31 0.5993692811272737 1102 32-33 0.5296883204098464 1102 34-35 0.5817512888667977 1102 36-37 0.48873291775716865 1102 38-39 0.5910736750743624 1102 40-41 0.5414843352734593 1102 42-43 0.4924949878667988 1102 44-45 0.49886019070229537 1102 46-47 0.6439081896189052 1102 48-49 0.5985162442959293 1102 50-51 0.5522908951910637 1102 52-53 0.4716324456287637 1102 54-55 0.49536793631970255 1102 56-57 0.5087255232658592 1102 58-59 0.43707992498760007 1102 60-61 0.5293755158260325 1102 62-63 0.5798899571933731 1102 64-65 0.4597534210400269 1102 66-67 0.41433699244882405 1102 68-69 0.6084265642549411 1102 70-71 0.5329610683380679 1102 72-73 0.4783704561722182 1102 74-75 0.3993181993700894 1102 76-77 0.4413964023689694 1102 78-79 0.3161444837058589 1102 80-81 0.22785359667627247 1102 82-83 0.31041168862471125 1102 84-85 0.3108133807024558 1102 86-87 0.29231604099111763 1102 88-89 0.0636017408511016 1102 90-91 0.10986402676136109 1102 92-93 0.2010436294326361 1102 94-95 0.05018305230746023 1102 96-97 0.010392024223044416 1102 98-99 -0.06876791444805974 1102 100-101 0.1356435210335114 1103 1 0.24557102544221365 1103 2 0.2967070842846802 1103 3 0.3824538776000104 1103 4 0.37013187173518247 1103 5 0.20644031579957556 1103 6 0.26787974928347325 1103 7 0.2577546768082115 1103 8 0.2293802480965681 1103 9 0.40915095485659236 1103 10-11 0.38951813528575485 1103 12-13 0.40835917727743976 1103 14-15 0.5076037590123477 1103 16-17 0.5254109015757109 1103 18-19 0.5144164574330787 1103 20-21 0.5328853129512723 1103 22-23 0.4756547871761967 1103 24-25 0.5709759375054162 1103 26-27 0.5670562301634661 1103 28-29 0.5618710455394762 1103 30-31 0.6082853410328823 1103 32-33 0.5706451769715741 1103 34-35 0.5213016119332963 1103 36-37 0.5658896042151369 1103 38-39 0.7234339370299097 1103 40-41 0.7312304294716796 1103 42-43 0.6587432503759345 1103 44-45 0.69505428692284 1103 46-47 0.6032058884872527 1103 48-49 0.647884286609326 1103 50-51 0.6184326751513751 1103 52-53 0.7063091619978863 1103 54-55 0.7457814564964877 1103 56-57 0.679339711500603 1103 58-59 0.7374310596657807 1103 60-61 0.8423788674136858 1103 62-63 0.6352472015549324 1103 64-65 0.623274039147212 1103 66-67 0.6832651834564842 1103 68-69 0.5466039297227496 1103 70-71 0.5858750572004752 1103 72-73 0.47872501552123126 1103 74-75 0.5086981256443366 1103 76-77 0.6048938951215561 1103 78-79 0.5798460797789993 1103 80-81 0.6087480465558599 1103 82-83 0.6475664720954839 1103 84-85 0.607692534660476 1103 86-87 0.641002697379065 1103 88-89 0.7092528149090818 1103 90-91 0.7461347685274156 1103 92-93 0.6209175415259232 1103 94-95 0.49880539902608234 1103 96-97 0.33379807826435126 1103 98-99 0.31863014422255276 1103 100-101 0.5463870664999 1104 1 0.23353810252838514 1104 2 0.27572393034212794 1104 3 0.20569252924353165 1104 4 0.24853333962433055 1104 5 0.21654980313797978 1104 6 0.2743333669960819 1104 7 0.27452791816989475 1104 8 0.30126400972874734 1104 9 0.37771224236271905 1104 10-11 0.41071835215820585 1104 12-13 0.3641348348170723 1104 14-15 0.4228155758940062 1104 16-17 0.4026510784607851 1104 18-19 0.4475263120554658 1104 20-21 0.4716455572046456 1104 22-23 0.4867000174802243 1104 24-25 0.5313661520626525 1104 26-27 0.39444267127171173 1104 28-29 0.47477032401086916 1104 30-31 0.4300262411435867 1104 32-33 0.5554629800453554 1104 34-35 0.6060523047250967 1104 36-37 0.5460028908041821 1104 38-39 0.6527393893355438 1104 40-41 0.656946940609771 1104 42-43 0.5636770466152328 1104 44-45 0.6392175539182077 1104 46-47 0.7906344469036739 1104 48-49 0.8160507827945764 1104 50-51 0.7310115368757906 1104 52-53 0.7983877850136878 1104 54-55 0.7822963418877791 1104 56-57 0.7967860384269798 1104 58-59 0.738470875085234 1104 60-61 0.7660832822502996 1104 62-63 0.7656205047713271 1104 64-65 0.8120639812805095 1104 66-67 0.6405602815053797 1104 68-69 0.6661878979240257 1104 70-71 0.5463430663498414 1104 72-73 0.5982439167931908 1104 74-75 0.6339152968026553 1104 76-77 0.5989686918729014 1104 78-79 0.6228220289985664 1104 80-81 0.6396724142810726 1104 82-83 0.7925972375405905 1104 84-85 0.6886910778192856 1104 86-87 0.5992637557699467 1104 88-89 0.5665432894396396 1104 90-91 0.5455451140912508 1104 92-93 0.5811927742040517 1104 94-95 0.5511892989308933 1104 96-97 0.5949049121880918 1104 98-99 0.5484443307352862 1104 100-101 0.727959168363455 1105 1 0.17488240368079033 1105 2 0.20566573052714077 1105 3 0.15306517318534318 1105 4 0.20883677219783436 1105 5 0.1841792220228271 1105 6 0.19302847988450367 1105 7 0.16711251775952007 1105 8 0.18624209188362073 1105 9 0.12272732735434033 1105 10-11 0.2098408983631117 1105 12-13 0.20771944388523167 1105 14-15 0.2734886260987679 1105 16-17 0.2864973913057085 1105 18-19 0.32263857854905353 1105 20-21 0.37086678817255603 1105 22-23 0.20279079715221116 1105 24-25 0.28574897278230793 1105 26-27 0.2203758114093901 1105 28-29 0.3558973806640182 1105 30-31 0.36457177822931897 1105 32-33 0.3139508341452313 1105 34-35 0.32421534801717655 1105 36-37 0.4060291263675069 1105 38-39 0.46487495454589833 1105 40-41 0.48034174800694274 1105 42-43 0.4112059282671865 1105 44-45 0.4092145424074545 1105 46-47 0.4807116603100354 1105 48-49 0.4523792223813601 1105 50-51 0.25579410031166105 1105 52-53 0.37506952087442613 1105 54-55 0.3897385565504621 1105 56-57 0.35866834406355963 1105 58-59 0.25420988502488484 1105 60-61 0.539438496013517 1105 62-63 0.4055361093025951 1105 64-65 0.42843284761382705 1105 66-67 0.4640842546109809 1105 68-69 0.49051916710037347 1105 70-71 0.39179556870575283 1105 72-73 0.3210115284356796 1105 74-75 0.29512702995453566 1105 76-77 0.2874574622303214 1105 78-79 0.37542446650971684 1105 80-81 0.4055163115983653 1105 82-83 0.46773654277790655 1105 84-85 0.4186686469697065 1105 86-87 0.39418461256886417 1105 88-89 0.42159533569551755 1105 90-91 0.5813919031905463 1105 92-93 0.4415055689009968 1105 94-95 0.38063078258301175 1105 96-97 0.3892882367662587 1105 98-99 0.4049371961486905 1105 100-101 0.6156490236629395 1106 1 0.1854277246740743 1106 2 0.16145348746971067 1106 3 0.231274460006194 1106 4 0.19141493868512072 1106 5 0.26189494137157965 1106 6 0.20702182835218252 1106 7 0.21002010177258512 1106 8 0.263351026876137 1106 9 0.30390131376443463 1106 10-11 0.22344816272017454 1106 12-13 0.24179877581193665 1106 14-15 0.2649810475123573 1106 16-17 0.36563922347467326 1106 18-19 0.3937906257558126 1106 20-21 0.29672369947382293 1106 22-23 0.39423206908333697 1106 24-25 0.3578810485953099 1106 26-27 0.3578718221696775 1106 28-29 0.25048527443589563 1106 30-31 0.2833563489483666 1106 32-33 0.32829215091089026 1106 34-35 0.30990253735715356 1106 36-37 0.36307034441967545 1106 38-39 0.5270327197705811 1106 40-41 0.5540322114306235 1106 42-43 0.3268853523178734 1106 44-45 0.4539000029692133 1106 46-47 0.45136272684215584 1106 48-49 0.3263820739609642 1106 50-51 0.20298641848954446 1106 52-53 0.31953357055677145 1106 54-55 0.3982539682593824 1106 56-57 0.3500206575641869 1106 58-59 0.34927500169157355 1106 60-61 0.577728817698052 1106 62-63 0.5658806923995883 1106 64-65 0.29072590782259766 1106 66-67 0.4460218008829244 1106 68-69 0.44572711146937394 1106 70-71 0.38055277679354305 1106 72-73 0.35943743581813337 1106 74-75 0.24323784231232892 1106 76-77 0.3056049497522402 1106 78-79 0.39628641362047645 1106 80-81 0.42059725278760496 1106 82-83 0.49486934860562215 1106 84-85 0.5635870662100544 1106 86-87 0.5997861511556337 1106 88-89 0.7175380527973694 1106 90-91 0.614971611107137 1106 92-93 0.5854830443938219 1106 94-95 0.6009223330179836 1106 96-97 0.5828705352660464 1106 98-99 0.5315962030444226 1106 100-101 0.8110926174284856 1107 1 0.1268317175553051 1107 2 0.2171815693818111 1107 3 0.199273050119519 1107 4 0.24523716969594744 1107 5 0.13737967016741948 1107 6 0.11835498020647606 1107 7 0.10661139899672634 1107 8 0.12907216207887728 1107 9 0.20373419468423037 1107 10-11 0.24132037023289143 1107 12-13 0.22215201148900832 1107 14-15 0.31605444893457246 1107 16-17 0.29167506644051855 1107 18-19 0.2531730491686588 1107 20-21 0.2649035011138139 1107 22-23 0.30303805044407284 1107 24-25 0.15573180605627357 1107 26-27 0.2770376777448931 1107 28-29 0.3329782506835457 1107 30-31 0.4171774499953784 1107 32-33 0.28393256432356395 1107 34-35 0.20555423114723226 1107 36-37 0.2269376327376449 1107 38-39 0.35516409989296704 1107 40-41 0.21396189686410594 1107 42-43 0.35978752313244655 1107 44-45 0.16125638842041923 1107 46-47 0.4191602026570749 1107 48-49 0.5191782978273451 1107 50-51 0.39776810434420184 1107 52-53 0.41597646372640185 1107 54-55 0.52100094239605 1107 56-57 0.3688180229508191 1107 58-59 0.33284349804358015 1107 60-61 0.3438662739440588 1107 62-63 0.32074258642438735 1107 64-65 0.2917117370491127 1107 66-67 0.17512019890240538 1107 68-69 0.34677245640367005 1107 70-71 0.17492616691611573 1107 72-73 0.3598072421577392 1107 74-75 0.4093999844155505 1107 76-77 0.47303188089892245 1107 78-79 0.6028588866746922 1107 80-81 0.5418060957817019 1107 82-83 0.5849778636533998 1107 84-85 0.5677470665182263 1107 86-87 0.6319926879963091 1107 88-89 0.6714600865781541 1107 90-91 0.5621355303352686 1107 92-93 0.4622964437101942 1107 94-95 0.4027839805052267 1107 96-97 0.4839101019762886 1107 98-99 0.5241959030903942 1107 100-101 0.6129764147746144 1108 1 0.31931456185386864 1108 2 0.2510644276295686 1108 3 0.3566603408366653 1108 4 0.29720665764610743 1108 5 0.24081216935453398 1108 6 0.2845600575147813 1108 7 0.33165733144925724 1108 8 0.34343362158316637 1108 9 0.4647357988409553 1108 10-11 0.4319159809535975 1108 12-13 0.4527401679072227 1108 14-15 0.5093102698558027 1108 16-17 0.5478875309249318 1108 18-19 0.5451716093897048 1108 20-21 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-1.7576804689691343 2316 76-77 -1.8342053567041958 2316 78-79 -1.7694425183833715 2316 80-81 -1.7362172637849476 2316 82-83 -1.5052130807329895 2316 84-85 -1.4942624197818013 2316 86-87 -1.5545023219361305 2316 88-89 -1.5648584777589711 2316 90-91 -1.8081719021032931 2316 92-93 -1.824993128747856 2316 94-95 -1.863496116540496 2316 96-97 -2.1010154390971856 2316 98-99 -2.106529376056052 2316 100-101 -1.869650926632385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12042.0 3 3609.0 4 3707.0 5 4173.0 6 4887.0 7 5976.0 8 7180.0 9 8691.0 10 10632.0 11 13333.0 12 16901.0 13 22737.0 14 20304.0 15 20141.0 16 23990.0 17 30740.0 18 27687.0 19 22070.0 20 22669.0 21 24494.0 22 27226.0 23 29813.0 24 34045.0 25 39473.0 26 45221.0 27 52439.0 28 61796.0 29 72780.0 30 87755.0 31 106371.0 32 131974.0 33 168305.0 34 225726.0 35 319589.0 36 487614.0 37 729810.0 38 621507.0 39 88301.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54460090657959 18.72133480833488 14.104964443503789 23.629099841581745 2 15.239842412907143 19.98620245773539 40.655592478082085 24.118362651275376 3 19.34033222913326 23.960875416277553 29.555513585184922 27.14327876940426 4 12.98928215168645 16.19926767197718 36.676781446148226 34.134668730188146 5 14.194875816590319 36.922080954652046 34.13909268843947 14.743950540318165 6 32.722055829859094 35.27230355617924 17.43479760141808 14.570843012543596 7 28.93894692808155 32.30892017745682 20.684177500853156 18.06795539360847 8 25.976155484733898 35.66772348728788 19.725474330817878 18.630646697160344 9 26.382247214946386 17.004342748814956 20.398286679769132 36.215123356469526 10-11 25.219266831327502 26.352600137139387 27.100110237167467 21.328022794365644 12-13 25.93732119872093 23.683561607266455 27.721102617175987 22.65801457683663 14-15 22.854866121475425 25.014554930120507 25.910224943331748 26.22035400507231 16-17 22.589551771564288 28.268946693793247 26.549238939806653 22.592262594835812 18-19 22.529576004900218 27.05155645329869 28.43457966753993 21.98428787426116 20-21 23.156360369564645 26.32014109689182 28.381629880481597 22.141868653061945 22-23 22.94147166765584 26.226330069249375 28.2479984408154 22.584199822279384 24-25 22.7442289426955 26.322958965958676 28.354963040455907 22.57784905088992 26-27 22.656001378652686 26.482423279409513 28.305036266714232 22.556539075223565 28-29 22.492077695362596 26.620058478397223 28.44060222591193 22.447261600328247 30-31 22.651123561793007 26.47037206887648 28.312548756861744 22.565955612468763 32-33 22.462672424957102 26.596439965060515 28.219967391829403 22.720920218152983 34-35 22.86525329549382 26.592905366952003 28.06990192676619 22.471939410787986 36-37 22.894009147801555 26.394695585362687 28.13121053916965 22.580084727666108 38-39 22.729418215060683 26.535750961424732 28.25820458145553 22.47662624205906 40-41 22.827887106328802 26.459316037735846 28.188452127283337 22.52434472865201 42-43 22.650585826513982 26.5444693068475 27.944002104951277 22.860942761687234 44-45 22.678982716574907 26.667182637935362 27.918574205536693 22.735260439953038 46-47 22.7871960557154 26.766002546438365 27.611719697196886 22.83508170064935 48-49 22.903119597183114 26.78996861217374 27.53840820539813 22.76850358524501 50-51 22.800910744370288 26.962522672893467 27.5343156652814 22.70225091745484 52-53 22.946160210464104 26.964897862601795 27.343305333557794 22.745636593376307 54-55 22.733165240494195 26.810139856853894 27.446154364950154 23.010540537701758 56-57 22.711239630458973 27.199279510513723 27.336278077106634 22.753202781920667 58-59 22.879197407766338 26.79368574761917 27.453735522815702 22.873381321798785 60-61 22.797324281701655 26.71401465458432 27.704069091421296 22.784591972292727 62-63 22.82199885355269 26.722241354245035 27.708979269424518 22.74678052277776 64-65 22.694368155506965 26.734242331631364 27.5869413980704 22.984448114791274 66-67 22.56738352205807 26.94348321826267 27.43281001341477 23.05632324626449 68-69 22.52567215461941 26.846366910025726 27.668840888082208 22.959120047272656 70-71 22.596426031036334 26.73511554809913 27.513809710450587 23.15464871041395 72-73 22.595910470382442 26.704972887699814 27.462950578007757 23.236166063909995 74-75 22.57450157412197 26.903509792954644 27.31022132815695 23.211767304766436 76-77 22.707543074586606 26.645565920276827 27.40055195284865 23.24633905228792 78-79 22.602075634979073 26.72941370890732 27.452178720326774 23.21633193578683 80-81 22.572399207659203 26.853222805430864 27.420463645113124 23.153914341796806 82-83 22.59926629411087 26.791265290261023 27.394708395716943 23.21476001991116 84-85 22.6780952154572 26.581298504663675 27.313896250582676 23.426710029296448 86-87 22.529936613111122 26.480495751888565 27.651440242375696 23.33812739262461 88-89 22.63918417135199 26.425550328731557 27.522192887081392 23.413072612835062 90-91 22.50449443265373 26.477993106923957 27.638307301148135 23.37920515927418 92-93 22.494136050269063 26.434663269391123 27.52719368672961 23.544006993610203 94-95 22.471221686878902 26.282340177162816 27.57666613396535 23.669772001992932 96-97 22.46771497111343 26.135619376850766 27.61850100592218 23.778164646113616 98-99 22.35780140544942 26.15355160399144 27.524469569677017 23.964177420882127 100-101 22.680657152207758 25.492496528269175 28.07755393344891 23.74929238607416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2583.0 1 1489.5 2 432.0 3 472.0 4 458.5 5 504.0 6 832.5 7 1642.5 8 2666.0 9 3179.0 10 3113.0 11 3124.0 12 3459.5 13 3887.5 14 4255.0 15 4537.5 16 4839.0 17 5111.0 18 5323.0 19 5549.0 20 6021.5 21 6872.5 22 7999.0 23 9592.0 24 11590.5 25 14464.0 26 18296.0 27 22712.5 28 27176.5 29 32475.0 30 38419.0 31 44420.5 32 49757.0 33 55248.0 34 62356.0 35 69697.5 36 77778.0 37 85903.0 38 92213.0 39 95875.5 40 98931.0 41 104593.5 42 110906.0 43 116855.0 44 123838.0 45 129017.5 46 131732.5 47 136646.5 48 149317.0 49 152806.5 50 144838.5 51 141135.0 52 137535.0 53 135022.0 54 131299.0 55 122801.0 56 112473.0 57 101444.5 58 91164.5 59 80140.5 60 67777.0 61 55208.0 62 43846.0 63 34705.0 64 26334.5 65 19004.5 66 13807.0 67 10776.0 68 8167.5 69 5676.5 70 3929.0 71 2798.5 72 1961.0 73 1302.0 74 910.0 75 623.5 76 413.0 77 237.0 78 162.5 79 118.0 80 74.5 81 55.5 82 56.0 83 45.5 84 33.5 85 27.0 86 25.5 87 26.5 88 23.5 89 16.5 90 11.5 91 10.0 92 9.0 93 12.0 94 10.0 95 2.5 96 2.5 97 3.0 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32414344715897964 2 0.08815271515863637 3 0.09747682449990869 4 0.10333533567893818 5 0.11474980581648393 6 0.05745741715020013 7 0.05985033016698683 8 0.06040042511337456 9 0.06389352802293675 10-11 0.06068922496022814 12-13 0.05949276845183479 14-15 0.05741616002922105 16-17 0.059231473352300605 18-19 0.06676777411781273 20-21 0.06843181133063567 22-23 0.08438456477588031 24-25 0.09313107442344548 26-27 0.08812521041131699 28-29 0.08560852603159305 30-31 0.08014883368869466 32-33 0.07144358116210858 34-35 0.32066409662307716 36-37 0.49908739248394274 38-39 0.47165140703285385 40-41 0.453058197844948 42-43 0.4840835528212169 44-45 0.49408152847181425 46-47 0.48797547456691026 48-49 0.5063623981499207 50-51 0.5021129146890753 52-53 0.500778934444085 54-55 0.49216994853311685 56-57 0.46991860795173246 58-59 0.4528244074927332 60-61 0.4132863332211139 62-63 0.4841385623158557 64-65 0.5076688736475916 66-67 0.5178593825294245 68-69 0.5299889760972744 70-71 0.5272247489916759 72-73 0.5155214790072766 74-75 0.5170617448571623 76-77 0.49108351101400105 78-79 0.4712250834494034 80-81 0.4770285851337941 82-83 0.4858713613969772 84-85 0.48739787487320313 86-87 0.4872878558839256 88-89 0.4941640427137724 90-91 0.508370244704236 92-93 0.5148888698189308 94-95 0.5212149617023898 96-97 0.5131560707378093 98-99 0.5155352313809364 100-101 0.49354518589908614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3635736.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.12294259325536 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93058979282705 37.284263552709525 2 13.952832465031548 14.26619706246235 3 5.264622056793064 8.074289135538534 4 2.517034639449384 5.147128695112242 5 1.3773998705575818 3.52083672552363 6 0.8602847586285841 2.638817299753299 7 0.5937074609255208 2.124645070945796 8 0.41714977826353694 1.7060739333564803 9 0.3234565267722523 1.4882444504632457 >10 1.616604738771572 14.669149320726754 >50 0.09839941146273287 3.438961550594949 >100 0.04538394637735552 4.071757457784173 >500 0.002049213990890648 0.6927515105138875 >1k 3.7748678248175757E-4 0.40964639358189875 >5k 1.078533664233593E-4 0.4672378409331731 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9396 0.2584346058129633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7552 0.2077158517560131 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4639 0.1275945228146378 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7504747319387325E-5 2 0.0 0.0 0.0 0.0 2.7504747319387325E-5 3 0.0 0.0 0.0 0.0 2.7504747319387325E-5 4 0.0 0.0 0.0 0.0 2.7504747319387325E-5 5 0.0 0.0 0.0 0.0 2.7504747319387325E-5 6 0.0 0.0 0.0 0.0 2.7504747319387325E-5 7 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 8 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 9 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 10-11 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 12-13 0.0 0.0 0.0 5.500949463877465E-5 5.500949463877465E-5 14-15 0.0 0.0 0.0 8.251424195816198E-5 1.3752373659693662E-4 16-17 0.0 0.0 0.0 1.2377136293724295E-4 1.6502848391632396E-4 18-19 0.0 0.0 0.0 1.6502848391632396E-4 1.6502848391632396E-4 20-21 0.0 0.0 0.0 1.7878085757601762E-4 2.0628560489540493E-4 22-23 0.0 0.0 0.0 3.025522205132606E-4 2.3379035221479227E-4 24-25 8.251424195816198E-5 0.0 0.0 4.125712097908099E-4 2.4754272587448596E-4 26-27 8.251424195816198E-5 0.0 0.0 7.838852986025389E-4 3.5756171515203525E-4 28-29 1.3752373659693662E-4 0.0 0.0 0.0028742460948759756 3.5756171515203525E-4 30-31 1.6502848391632396E-4 0.0 0.0 0.008347690811434053 3.5756171515203525E-4 32-33 1.6502848391632396E-4 0.0 0.0 0.0191020470133145 3.5756171515203525E-4 34-35 2.0628560489540496E-4 0.0 0.0 0.03469723874340711 3.850664624714226E-4 36-37 2.7504747319387324E-4 0.0 0.0 0.060647967839249056 3.850664624714226E-4 38-39 3.025522205132606E-4 2.7504747319387325E-5 0.0 0.10260645987497442 3.988188361311163E-4 40-41 3.025522205132606E-4 2.7504747319387325E-5 0.0 0.14676533169625078 4.125712097908099E-4 42-43 3.163045941729542E-4 5.500949463877465E-5 0.0 0.19473361102126227 4.125712097908099E-4 44-45 3.850664624714226E-4 8.251424195816198E-5 0.0 0.2547764744194848 4.125712097908099E-4 46-47 4.125712097908099E-4 8.251424195816198E-5 0.0 0.3262200555815934 4.6758070442958453E-4 48-49 4.125712097908099E-4 9.626661561785565E-5 0.0 0.4055987563453452 5.088378254086655E-4 50-51 4.125712097908099E-4 1.100189892775493E-4 0.0 0.489061912086026 5.500949463877465E-4 52-53 4.125712097908099E-4 1.100189892775493E-4 0.0 0.5785211027423334 5.913520673668275E-4 54-55 4.400759571101972E-4 1.100189892775493E-4 0.0 0.6784871068746465 6.601139356652958E-4 56-57 4.400759571101972E-4 1.100189892775493E-4 0.0 0.8014608321396273 6.738663093249894E-4 58-59 4.400759571101972E-4 1.100189892775493E-4 0.0 0.9388057878789879 6.876186829846831E-4 60-61 4.400759571101972E-4 1.100189892775493E-4 0.0 1.0886378989013503 6.876186829846831E-4 62-63 4.400759571101972E-4 1.100189892775493E-4 0.0 1.2439709593875903 6.876186829846831E-4 64-65 4.400759571101972E-4 1.100189892775493E-4 0.0 1.4080642818950553 7.151234303040705E-4 66-67 4.950854517489719E-4 1.100189892775493E-4 0.0 1.6000061610633995 7.151234303040705E-4 68-69 4.950854517489719E-4 1.100189892775493E-4 0.0 1.8206905011805037 7.151234303040705E-4 70-71 4.950854517489719E-4 1.100189892775493E-4 0.0 2.067449341756387 7.426281776234578E-4 72-73 5.088378254086655E-4 1.100189892775493E-4 0.0 2.3215381974928873 7.426281776234578E-4 74-75 5.225901990683592E-4 1.100189892775493E-4 0.0 2.587041523366933 7.426281776234578E-4 76-77 5.225901990683592E-4 1.100189892775493E-4 0.0 2.8802146250442826 7.563805512831515E-4 78-79 5.225901990683592E-4 1.100189892775493E-4 0.0 3.207823670365505 7.701329249428452E-4 80-81 6.326091883459085E-4 1.100189892775493E-4 0.0 3.5722478199737275 7.976376722622326E-4 82-83 6.463615620056022E-4 1.100189892775493E-4 0.0 3.951070704803649 8.801519142203944E-4 84-85 6.601139356652958E-4 1.100189892775493E-4 0.0 4.34115678366086 8.801519142203944E-4 86-87 6.601139356652958E-4 1.100189892775493E-4 0.0 4.7541955741561 8.939042878800881E-4 88-89 6.601139356652958E-4 1.2377136293724295E-4 0.0 5.200597623149756 9.076566615397818E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10655 0.0 32.606705 1 GTATCAA 15700 0.0 29.32388 1 ATCAACG 18235 0.0 24.934402 3 CAACGCA 18375 0.0 24.744429 5 TATCAAC 18520 0.0 24.627415 2 TCAACGC 18545 0.0 24.594215 4 AACGCAG 18770 0.0 24.262808 6 GTACATG 12195 0.0 23.234957 1 TACATGG 12265 0.0 22.474443 2 ACGCAGA 20975 0.0 21.351057 7 CGCAGAG 21030 0.0 21.227686 8 GAGTACT 9730 0.0 21.1404 12-13 ACATGGG 12635 0.0 20.916668 3 GTACTTT 10035 0.0 20.380346 14-15 GCAGAGT 21950 0.0 19.281164 9 AGTACTT 9930 0.0 18.307045 12-13 CAGAGTA 21015 0.0 17.210175 10-11 AGTACAT 9520 0.0 17.163889 2 AGAGTAC 18310 0.0 16.90872 10-11 CATGGGG 7080 0.0 16.590212 4 >>END_MODULE