FastQCFastQC Report
Fri 27 May 2016
SRR939015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635736
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93370.25681182572111944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82920.22806936477235973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.1377987840701305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118550.030.1792341
GTATCAA167000.026.1020411
GTACATG126700.025.0043131
TACATGG127900.024.0813272
ACATGGG132400.022.4384383
ATCAACG196150.021.9444793
TCAACGC198000.021.7394434
CAACGCA199200.021.656135
TATCAAC200950.021.632852
AACGCAG204300.021.161986
GAGTACT102550.019.71506312-13
ACGCAGA225850.018.911627
GTATAGG9150.018.7435251
GTACTTT107450.018.48473514-15
CGCAGAG227600.018.4742898
AGTACTT104250.018.4375512-13
CATGGGG74000.018.3417434
CATGGGA73750.017.4386794
GCAGAGT240500.016.8913759
AGAGTAC193900.016.85195710-11
Produced by FastQC (version 0.11.5)