##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939015_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3635736 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19186651616069 33.0 31.0 34.0 30.0 34.0 2 32.4372553452726 34.0 31.0 34.0 31.0 34.0 3 32.52887090811874 34.0 31.0 34.0 31.0 34.0 4 36.07533990366737 37.0 35.0 37.0 35.0 37.0 5 35.99549142181941 37.0 35.0 37.0 35.0 37.0 6 36.03249960943259 37.0 35.0 37.0 35.0 37.0 7 35.96580389775275 37.0 35.0 37.0 35.0 37.0 8 35.933573009701476 37.0 35.0 37.0 35.0 37.0 9 37.61769418901702 39.0 37.0 39.0 35.0 39.0 10-11 37.52431364653539 39.0 37.0 39.0 35.0 39.0 12-13 37.429664447583654 39.0 37.0 39.0 34.0 39.0 14-15 38.76748903110677 40.0 38.0 41.0 34.5 41.0 16-17 38.85319822451355 40.0 38.0 41.0 35.0 41.0 18-19 38.87410279514244 40.0 38.0 41.0 35.0 41.0 20-21 38.84345246739588 40.0 38.0 41.0 34.0 41.0 22-23 38.76988785764423 40.0 38.0 41.0 34.0 41.0 24-25 38.69672137911003 40.0 38.0 41.0 34.0 41.0 26-27 38.542375188957614 40.0 38.0 41.0 34.0 41.0 28-29 38.38151436187886 40.0 38.0 41.0 34.0 41.0 30-31 38.197655165281525 40.0 38.0 41.0 33.5 41.0 32-33 38.126882562430275 40.0 38.0 41.0 33.0 41.0 34-35 38.049194165913036 40.0 38.0 41.0 33.0 41.0 36-37 37.92876738024984 40.0 37.0 41.0 33.0 41.0 38-39 37.79347042799587 40.0 37.0 41.0 33.0 41.0 40-41 37.619680719392164 40.0 37.0 41.0 32.5 41.0 42-43 37.44419548063996 40.0 37.0 41.0 32.0 41.0 44-45 37.21214837931027 40.0 36.5 41.0 31.0 41.0 46-47 36.924851116802756 40.0 36.0 41.0 31.0 41.0 48-49 36.617506881687774 39.0 36.0 41.0 30.0 41.0 50-51 36.35305533735122 39.0 35.5 41.0 30.0 41.0 52-53 36.078809902589185 39.0 35.0 40.0 29.5 41.0 54-55 35.76636559970251 38.5 35.0 40.0 28.5 41.0 56-57 35.62169337927726 38.0 35.0 40.0 28.0 41.0 58-59 35.32953011439774 38.0 34.0 40.0 28.0 41.0 60-61 34.95562507838853 38.0 34.0 40.0 27.0 41.0 62-63 34.51936375468406 37.0 34.0 40.0 26.0 41.0 64-65 34.08837179047104 36.5 33.0 39.5 26.0 41.0 66-67 33.62515966505819 36.0 33.0 39.0 25.5 40.0 68-69 33.126491444923396 35.5 32.0 39.0 24.5 40.0 70-71 32.59580783643257 35.0 32.0 38.0 23.0 40.0 72-73 32.0716299258252 35.0 31.0 37.0 22.0 39.0 74-75 31.5245864936288 35.0 31.0 37.0 20.0 39.0 76-77 29.646199146472682 32.5 28.5 35.0 18.0 37.0 78-79 30.604978606807535 34.0 30.0 36.0 18.0 37.5 80-81 30.586443707683948 34.0 30.0 35.0 18.0 37.0 82-83 30.162624293953137 34.0 30.0 35.0 15.0 37.0 84-85 29.6951692862188 34.0 29.5 35.0 9.0 36.0 86-87 28.963895205812523 34.0 29.0 35.0 4.5 36.0 88-89 28.258218005927823 33.0 27.5 35.0 2.0 35.0 90-91 27.657096939931833 33.0 26.5 35.0 2.0 35.0 92-93 27.21063905630112 33.0 26.0 34.0 2.0 35.0 94-95 26.617710966912888 32.0 25.0 34.0 2.0 35.0 96-97 25.721666672167615 32.0 23.5 34.0 2.0 35.0 98-99 24.440453597290894 32.0 10.5 34.0 2.0 35.0 100-101 18.80659830636768 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16040115793160226 1101 2 0.20445314451527707 1101 3 0.20560482385157997 1101 4 0.15260471332243952 1101 5 0.16450405485678488 1101 6 0.1918418765337293 1101 7 0.19058357652470193 1101 8 0.17254013037353388 1101 9 0.21475240627297154 1101 10-11 0.2705317125570801 1101 12-13 0.23893949445859164 1101 14-15 0.32492567194810107 1101 16-17 0.32542766087833286 1101 18-19 0.32704788059920276 1101 20-21 0.3287603699172337 1101 22-23 0.32331861232248826 1101 24-25 0.2920446093427387 1101 26-27 0.29398388199308556 1101 28-29 0.31967840016473303 1101 30-31 0.20687279581326123 1101 32-33 0.3384896479703343 1101 34-35 0.39891294363589935 1101 36-37 0.41380026306180895 1101 38-39 0.34180791306093283 1101 40-41 0.3390110289283541 1101 42-43 0.3055382661852306 1101 44-45 0.2612045782499379 1101 46-47 0.19543345023820535 1101 48-49 0.16972796253021727 1101 50-51 0.12582674373722114 1101 52-53 0.08421722124263908 1101 54-55 0.22639118817426862 1101 56-57 0.09923398473999612 1101 58-59 0.08664944522512741 1101 60-61 0.2477651950712385 1101 62-63 0.28576949905576754 1101 64-65 0.16450728375928492 1101 66-67 0.06445983809408773 1101 68-69 0.22273245949783416 1101 70-71 0.20808049428115538 1101 72-73 0.24358890153657597 1101 74-75 0.33003934955379677 1101 76-77 0.06176954901644649 1101 78-79 0.41805589646598307 1101 80-81 0.5959821761374009 1101 82-83 0.5436089484430191 1101 84-85 0.5645662192704144 1101 86-87 0.749132963500454 1101 88-89 0.6601540639231374 1101 90-91 0.6332858906188648 1101 92-93 0.6438201651100606 1101 94-95 0.5920793186891409 1101 96-97 0.6457552196109333 1101 98-99 1.0310039486124616 1101 100-101 1.4982089482133318 1102 1 0.10018311873114527 1102 2 0.13086300745374757 1102 3 0.13944174240099727 1102 4 0.11965804292054827 1102 5 0.06102211222486176 1102 6 0.05458098532172784 1102 7 0.15653367381162298 1102 8 0.1276075100615799 1102 9 0.12271155824720381 1102 10-11 0.2785283265838743 1102 12-13 0.21280854265548754 1102 14-15 0.2718706684324559 1102 16-17 0.27971485561721465 1102 18-19 0.16918927462440791 1102 20-21 0.2290884272437097 1102 22-23 0.2560631332589409 1102 24-25 0.29122403263862395 1102 26-27 0.3004842675349906 1102 28-29 0.18936393735080514 1102 30-31 0.17298732048030985 1102 32-33 0.2962737632279442 1102 34-35 0.13521034414043953 1102 36-37 0.1721547688400804 1102 38-39 0.22219882550547254 1102 40-41 0.2648962324403925 1102 42-43 0.23063721731709563 1102 44-45 0.3063887369156859 1102 46-47 0.3740715338730567 1102 48-49 0.33815013923322823 1102 50-51 0.25687779267469324 1102 52-53 0.24099296413889704 1102 54-55 0.36071734968859204 1102 56-57 0.338643717315378 1102 58-59 0.2518039636236651 1102 60-61 0.15925541853299308 1102 62-63 0.30524664599022344 1102 64-65 0.3038893488493031 1102 66-67 0.17760485801606762 1102 68-69 0.1995673546456942 1102 70-71 0.20577062329900997 1102 72-73 0.35548585090160856 1102 74-75 0.3494253116680639 1102 76-77 0.10254337876502362 1102 78-79 0.35423666361115735 1102 80-81 0.31140154656689134 1102 82-83 0.3695364332813753 1102 84-85 0.6062382021623343 1102 86-87 0.7136998598742785 1102 88-89 0.4803537205412134 1102 90-91 0.431033192172265 1102 92-93 0.6720871126583354 1102 94-95 0.7565652061913433 1102 96-97 0.7348038706996718 1102 98-99 0.854870327139718 1102 100-101 0.8534787027285411 1103 1 0.05904275983269969 1103 2 0.07999481809134323 1103 3 0.061946207712793466 1103 4 0.0336001250286202 1103 5 0.08292959032903724 1103 6 0.0026573265305032123 1103 7 0.08821360901826125 1103 8 0.084555690555149 1103 9 0.03929505013338286 1103 10-11 0.05502759981222738 1103 12-13 0.10828061587707083 1103 14-15 0.1425502431660135 1103 16-17 0.02123041015438787 1103 18-19 0.09020736218229786 1103 20-21 0.12030066892378954 1103 22-23 0.05765641723230175 1103 24-25 0.12472580446445392 1103 26-27 0.08466036410929689 1103 28-29 0.09792910971943058 1103 30-31 0.1380640631170067 1103 32-33 0.18507602520538313 1103 34-35 0.1465136997176515 1103 36-37 0.16071998710635427 1103 38-39 0.23997816301879027 1103 40-41 0.28901200537976734 1103 42-43 0.2680211183278658 1103 44-45 0.3190973549762717 1103 46-47 0.34725185973930905 1103 48-49 0.24733563599938435 1103 50-51 0.20444864187655298 1103 52-53 0.25704663826345353 1103 54-55 0.41333521044147403 1103 56-57 0.5156980520130503 1103 58-59 0.4119092669559379 1103 60-61 0.4349473344386041 1103 62-63 0.4006762462322726 1103 64-65 0.3488102619018534 1103 66-67 0.3197531469693118 1103 68-69 0.39615228701197225 1103 70-71 0.3514152540329931 1103 72-73 0.17306855228902407 1103 74-75 0.21456865885660292 1103 76-77 0.13508357716291286 1103 78-79 0.38900192713957793 1103 80-81 0.3172918921373622 1103 82-83 0.3318323961593528 1103 84-85 0.4935064234412643 1103 86-87 0.5570077529638233 1103 88-89 0.47246965165095745 1103 90-91 0.48470775805993327 1103 92-93 0.6320242893667576 1103 94-95 0.8031616482760988 1103 96-97 0.49835276882065926 1103 98-99 0.7065761662741039 1103 100-101 1.2072803874547553 1104 1 0.08933892648577313 1104 2 0.007214665348996618 1104 3 -0.013453246088651838 1104 4 0.05116282781622061 1104 5 0.023325079506797408 1104 6 0.06243881247388572 1104 7 0.0701472544304238 1104 8 0.06237535706267039 1104 9 0.11612156066020418 1104 10-11 0.09859374028095402 1104 12-13 0.09892660623903282 1104 14-15 0.17648344769886393 1104 16-17 0.1087617509418024 1104 18-19 0.12757296628826253 1104 20-21 0.1549855415164174 1104 22-23 0.02946291242704291 1104 24-25 0.031644033819709705 1104 26-27 -0.032029532706587815 1104 28-29 0.11596983581614495 1104 30-31 -0.05365999491905882 1104 32-33 0.05960555624035635 1104 34-35 0.14365915335320523 1104 36-37 0.11983007584847627 1104 38-39 0.12298345166573199 1104 40-41 0.17434463808556444 1104 42-43 0.022196311229890853 1104 44-45 0.13370872921233 1104 46-47 0.25837788039830656 1104 48-49 0.23680622237059623 1104 50-51 0.22029298030908961 1104 52-53 0.08107470804152683 1104 54-55 0.2457082978936782 1104 56-57 0.08139062821841492 1104 58-59 0.267800564403494 1104 60-61 0.36938099586857476 1104 62-63 0.241816572091615 1104 64-65 0.22122769357032013 1104 66-67 0.23156936194248345 1104 68-69 0.20342529780381824 1104 70-71 0.07774677969187849 1104 72-73 0.14205340256062726 1104 74-75 0.20946928178478785 1104 76-77 0.22096006203526386 1104 78-79 0.32018496945966035 1104 80-81 0.26243428005124514 1104 82-83 0.2564223792636007 1104 84-85 0.5015868009697151 1104 86-87 0.5180360823169501 1104 88-89 0.557254155002834 1104 90-91 0.6531875105995368 1104 92-93 0.6581437912112342 1104 94-95 0.9649565206037884 1104 96-97 0.7197384718976174 1104 98-99 0.9684692580623704 1104 100-101 0.950504595365544 1105 1 -0.015913208136574042 1105 2 0.011016552174396566 1105 3 0.06420082860513077 1105 4 0.016208101082966664 1105 5 -0.006957398871769271 1105 6 -0.1057028459323277 1105 7 0.024658226489890467 1105 8 0.03473156740148653 1105 9 -9.462222775553641E-4 1105 10-11 0.11049639980579684 1105 12-13 0.12731732872390467 1105 14-15 0.11710455391605734 1105 16-17 -0.028716084688241494 1105 18-19 0.03647588838492055 1105 20-21 0.0249338403121655 1105 22-23 0.05892100844384629 1105 24-25 -0.08120464084117884 1105 26-27 -0.12102986079663935 1105 28-29 0.08976312378891294 1105 30-31 0.04908610878300834 1105 32-33 0.07702534171635733 1105 34-35 0.06749316573149855 1105 36-37 0.04850707477407212 1105 38-39 0.16204674544034958 1105 40-41 0.2784248859506988 1105 42-43 0.2634983913673352 1105 44-45 0.20808788989048566 1105 46-47 0.24122939695421053 1105 48-49 0.2549849135457478 1105 50-51 0.22423089230279913 1105 52-53 0.22464578110882627 1105 54-55 0.41842816769531055 1105 56-57 0.3725424404544526 1105 58-59 0.17481148117155243 1105 60-61 0.10139033703948996 1105 62-63 0.17161386632154318 1105 64-65 0.07770101991615519 1105 66-67 0.028757291022486697 1105 68-69 0.12777626402318276 1105 70-71 0.38173126949688907 1105 72-73 0.2985538092226605 1105 74-75 0.22937119400265615 1105 76-77 -0.10454255345600671 1105 78-79 0.09430141698113559 1105 80-81 -0.03077054774471577 1105 82-83 -0.0477909670669554 1105 84-85 0.03138167568105743 1105 86-87 0.19866366220138687 1105 88-89 0.4194122691286175 1105 90-91 0.21594225638961362 1105 92-93 0.4992461488200668 1105 94-95 0.41786412509248194 1105 96-97 0.5516486128067228 1105 98-99 0.7506989916010376 1105 100-101 0.7459563743602295 1106 1 0.009052649139739799 1106 2 0.026522258563765888 1106 3 0.034171382524796456 1106 4 -0.008424888863501678 1106 5 0.024373960622362745 1106 6 0.049789965683821436 1106 7 0.06395772891702478 1106 8 0.06111836925223457 1106 9 0.02608273411149753 1106 10-11 0.08860524500003919 1106 12-13 0.03180313995113693 1106 14-15 -0.04415230531514425 1106 16-17 -0.12158001317522604 1106 18-19 -0.07514328102630685 1106 20-21 0.007277578400980644 1106 22-23 0.013184052127151347 1106 24-25 0.05614673979108176 1106 26-27 0.051121822048799004 1106 28-29 0.1045310630930345 1106 30-31 0.07877800381076128 1106 32-33 0.07011644690695817 1106 34-35 0.03477857370635462 1106 36-37 0.13125060897558427 1106 38-39 0.12255004213513132 1106 40-41 0.25823263043779576 1106 42-43 0.1598769207946873 1106 44-45 0.23570400745256137 1106 46-47 0.24672461503480037 1106 48-49 0.2852126910579287 1106 50-51 0.15593696576683413 1106 52-53 0.20406518037778199 1106 54-55 0.3305937498917402 1106 56-57 0.32669178273057753 1106 58-59 0.2073090068738921 1106 60-61 0.29224808172038763 1106 62-63 0.33880080306349925 1106 64-65 0.28392185970441375 1106 66-67 0.3856853083412801 1106 68-69 0.43757755143320765 1106 70-71 0.5612970431768289 1106 72-73 0.5296360108178462 1106 74-75 0.46236887669045856 1106 76-77 0.24123973806664623 1106 78-79 0.4111538067133793 1106 80-81 0.44555460662432367 1106 82-83 0.4233338822463786 1106 84-85 0.5009361858153447 1106 86-87 0.5978052650294536 1106 88-89 0.7626825509401378 1106 90-91 0.9500407754287821 1106 92-93 0.9498180959695866 1106 94-95 0.9114893361830241 1106 96-97 0.7789561376433731 1106 98-99 0.8375327078152459 1106 100-101 1.1260259389484446 1107 1 0.024895255452641152 1107 2 0.05156187149979985 1107 3 0.05647404333276995 1107 4 0.03950580069691512 1107 5 0.013883594847939662 1107 6 0.04774383234295243 1107 7 0.015348419042425121 1107 8 0.020785081288657636 1107 9 -0.010536439833593647 1107 10-11 0.06305313569144744 1107 12-13 0.04201390388372772 1107 14-15 0.07668641032690005 1107 16-17 0.04945750547560124 1107 18-19 0.12739518910541392 1107 20-21 0.10973399775108561 1107 22-23 0.058454351028267126 1107 24-25 0.023234136071955902 1107 26-27 0.09907145134361883 1107 28-29 0.07418029921181812 1107 30-31 0.02359660609688774 1107 32-33 0.061076896889368015 1107 34-35 -0.016867032403666826 1107 36-37 0.10159339248736643 1107 38-39 9.232409342843084E-4 1107 40-41 -4.8205498284659143E-4 1107 42-43 0.04573404329046582 1107 44-45 0.024549242215393008 1107 46-47 -0.0420002400601831 1107 48-49 -0.015137469319888908 1107 50-51 -0.11274644020091529 1107 52-53 -0.032236576459148125 1107 54-55 -0.1380133193159807 1107 56-57 0.037073871006434445 1107 58-59 -0.01261581843113646 1107 60-61 -0.02897350546787436 1107 62-63 0.19868079577009468 1107 64-65 0.1344040525019139 1107 66-67 0.137698216893817 1107 68-69 0.09386406659912438 1107 70-71 0.18296798358921507 1107 72-73 0.2164686259393278 1107 74-75 0.11495395782955953 1107 76-77 0.0675559533042076 1107 78-79 0.2819668413596119 1107 80-81 0.2406140003473709 1107 82-83 0.1388536142395047 1107 84-85 0.27345649306277764 1107 86-87 0.3091831339176956 1107 88-89 0.49669051333289005 1107 90-91 0.5503884640107586 1107 92-93 0.7770487151640779 1107 94-95 0.8680481662082151 1107 96-97 0.9323522567312885 1107 98-99 0.9225841532700976 1107 100-101 1.1398262640698498 1108 1 0.06015101186705607 1108 2 0.03433200360123578 1108 3 0.0940032555867063 1108 4 0.08702636510860629 1108 5 0.05496878608116873 1108 6 0.10736990239472988 1108 7 0.09643217278881622 1108 8 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-0.567834116330932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 37.0 5 75.0 6 164.0 7 447.0 8 917.0 9 1847.0 10 3068.0 11 4660.0 12 6855.0 13 9255.0 14 11788.0 15 15705.0 16 20417.0 17 27806.0 18 28126.0 19 21637.0 20 21284.0 21 23091.0 22 24979.0 23 28529.0 24 32493.0 25 38534.0 26 46195.0 27 56074.0 28 67521.0 29 81438.0 30 98378.0 31 119308.0 32 148796.0 33 188189.0 34 252775.0 35 362542.0 36 563698.0 37 808789.0 38 496271.0 39 24031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34145350087823 19.524602807086723 14.626021371942993 23.50792232009205 2 15.670664756737013 20.585845616953485 39.74898067406434 23.99450895224516 3 19.324010324181952 24.157997170311596 29.833766808151086 26.684225697355362 4 13.391951450820411 16.85501917630983 36.21387801534544 33.53915135752431 5 14.073849146362663 37.114906032781256 34.13699454525851 14.674250275597567 6 32.67082648465125 35.727841625464556 17.0899097184174 14.511422171466796 7 28.89398460174226 32.41715020012454 20.541535469021955 18.147329729111245 8 25.976336015596292 35.98143539574931 19.419121740412397 18.623106848242006 9 26.12898736321889 16.96685347891046 20.238900734266736 36.665258423603916 10-11 25.180596170899094 26.587257710680863 26.944489368865064 21.287656749554973 12-13 25.852482138417095 23.7366519461259 27.73116639932052 22.679699516136484 14-15 22.71564811537136 25.185266783342865 25.83862048137595 26.260464619909822 16-17 22.451025654672538 28.46364484404248 26.55379562436299 22.531533876921987 18-19 22.403175272896505 27.167569880950648 28.4759493239164 21.953305522236455 20-21 23.04485254154867 26.407252891849133 28.476888861017414 22.071005705584785 22-23 22.844439200205954 26.315744597517533 28.307239579551428 22.53257662272508 24-25 22.674588590526312 26.443622235186137 28.410034510234937 22.47175466405261 26-27 22.60994747693452 26.571098671630722 28.37103684095875 22.447917010476008 28-29 22.420109710936106 26.708801189085236 28.491452624723028 22.37963647525563 30-31 22.61495334094665 26.48412866060682 28.3706792792436 22.530238719202934 32-33 22.47301509240495 26.608697661216326 28.288880160715742 22.629407085662987 34-35 22.786490390421204 26.63150476208291 28.17844242491429 22.403562422581597 36-37 22.78363028720397 26.46785008038718 28.211470551661833 22.537049080747025 38-39 22.685496047557496 26.601669661795697 28.367635881801657 22.345198408845153 40-41 22.776602262810915 26.505103461744493 28.236161377078535 22.482132898366054 42-43 22.589401007029622 26.65223232784864 28.022632654512318 22.735734010609416 44-45 22.671328428975283 26.703272402011397 27.95799022905769 22.66740893995563 46-47 22.772404813168993 26.87526886215884 27.58976833021059 22.762557994461577 48-49 22.878385797446825 26.8661700261963 27.559282742898887 22.69616143345799 50-51 22.775146319385673 27.05535450975064 27.500535658637332 22.668963512226355 52-53 22.94209480556344 27.097470773455502 27.259597506529627 22.700836914451433 54-55 22.732151069274558 26.9134640138888 27.3875220863121 22.966862830524548 56-57 22.687359588264936 27.2357921477247 27.280294828887463 22.796553435122902 58-59 22.82767641364086 26.922888091031478 27.40388086561921 22.84555462970845 60-61 22.806332568781475 26.80143004845585 27.64641617347617 22.74582120928651 62-63 22.782954399489725 26.832427359591033 27.6341749266919 22.75044331422734 64-65 22.64381258760339 26.85948856122501 27.467458847034855 23.02924000413675 66-67 22.54936517958557 27.00320948104107 27.40016907640193 23.04725626297143 68-69 22.465341107098684 26.974263452969993 27.556566960433727 23.0038284794976 70-71 22.542253841692787 26.84533207801615 27.40900084975952 23.20341323053155 72-73 22.59780413099301 26.839957576677737 27.24155439228811 23.320683900041146 74-75 22.53989288551204 27.031583151251905 27.096439345431023 23.332084617805034 76-77 22.626070361984972 26.805120889237354 27.29480874437289 23.274000004404783 78-79 22.511563735564156 26.89246361994399 27.289673277796595 23.30629936669526 80-81 22.45121197185268 27.01948550606646 27.26284804583841 23.266454476242448 82-83 22.50925259699824 26.912171290764785 27.267518873757613 23.31105723847936 84-85 22.520126632065637 26.764900033830898 27.15072351357224 23.564249820531227 86-87 22.427793628552813 26.639570995297902 27.407125677030443 23.525509699118842 88-89 22.479699749109507 26.58783128622677 27.297297222635063 23.63517174202866 90-91 22.35368636014854 26.624331907019073 27.41804997612992 23.60393175670247 92-93 22.309691902822426 26.63865033104714 27.2841729982595 23.767484767870933 94-95 22.243300253018887 26.522438476033617 27.278923408633887 23.95533786231361 96-97 22.227521470205758 26.404653693227452 27.312750430724343 24.055074405842447 98-99 22.052550020133474 26.411557384804617 27.18991422919596 24.345978365865946 100-101 22.35065616971159 25.673518402539692 27.954661537560227 24.021163890188493 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 623.0 1 432.5 2 299.5 3 362.5 4 354.0 5 399.0 6 630.0 7 1232.5 8 2047.5 9 2515.0 10 2523.0 11 2499.5 12 2695.0 13 2981.0 14 3248.5 15 3458.5 16 3637.5 17 3884.5 18 4270.0 19 4616.5 20 5191.5 21 6255.5 22 7682.0 23 9539.5 24 11712.0 25 14572.5 26 18481.5 27 23048.0 28 27477.5 29 32654.5 30 38684.5 31 45040.0 32 50329.5 33 55286.0 34 62604.0 35 70192.5 36 78174.0 37 86542.5 38 92234.5 39 95514.0 40 99379.0 41 105387.5 42 112186.0 43 118244.5 44 124855.5 45 130185.5 46 133039.5 47 138303.0 48 150506.0 49 153972.5 50 146578.0 51 142407.5 52 138388.0 53 136133.5 54 132675.5 55 124010.5 56 113362.5 57 101521.0 58 90914.0 59 80198.5 60 67701.5 61 54785.0 62 43336.0 63 34243.5 64 25722.0 65 18279.5 66 13109.5 67 10122.0 68 7500.0 69 5101.0 70 3455.5 71 2323.5 72 1517.0 73 999.5 74 699.5 75 446.5 76 256.5 77 117.5 78 61.5 79 34.5 80 18.5 81 12.5 82 10.0 83 5.0 84 2.5 85 2.5 86 3.5 87 3.5 88 2.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3921901920271439 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.076566615397818E-4 16-17 0.0022691416538494543 18-19 4.675807044295846E-4 20-21 0.0 22-23 0.0 24-25 8.251424195816198E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012569669524960008 36-37 0.022773930780452707 38-39 0.01069934670724167 40-41 0.005872263552689194 42-43 0.00529466385898206 44-45 0.001595275344524465 46-47 0.0012102088820530423 48-49 0.0029705127104938315 50-51 6.876186829846831E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.288758039637642E-4 60-61 0.001512761102566303 62-63 0.0014577516079275284 64-65 8.801519142203944E-4 66-67 0.0027917318529178136 68-69 0.0024479225114254722 70-71 4.125712097908099E-5 72-73 0.0 74-75 0.0 76-77 0.09131576110036592 78-79 0.003424341041263722 80-81 2.200379785550986E-4 82-83 0.0 84-85 1.6502848391632396E-4 86-87 0.09410749295328374 88-89 0.4354001500659014 90-91 0.38635918559543375 92-93 0.0 94-95 0.0010726851454561058 96-97 0.0 98-99 0.0 100-101 4.125712097908099E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3635736.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.29277432166664 #Duplication Level Percentage of deduplicated Percentage of total 1 71.19347385519576 35.093238399196395 2 14.811346942729578 14.601847644957521 3 5.572732279795555 8.240863038690874 4 2.671976364186104 5.268365116506118 5 1.496106963131016 3.6873631447345603 6 0.92601779710515 2.738759177433063 7 0.6452628924568669 2.226475869521553 8 0.44296999288065314 1.7468175912269048 9 0.3514239189241817 1.5590393934088824 >10 1.7281082436262867 15.235759187231643 >50 0.1084465137346408 3.6575723313848023 >100 0.049283202621993726 4.278279583145089 >500 0.0023479100394347297 0.7516161532972079 >1k 3.354157151851296E-4 0.29016417372744563 >5k 1.6770785759256476E-4 0.6238391955380477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9337 0.25681182572111944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8292 0.22806936477235973 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5010 0.1377987840701305 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7504747319387325E-5 2 0.0 0.0 0.0 0.0 2.7504747319387325E-5 3 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 4 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 5 0.0 0.0 0.0 2.7504747319387325E-5 2.7504747319387325E-5 6 0.0 0.0 0.0 2.7504747319387325E-5 5.500949463877465E-5 7 0.0 0.0 0.0 2.7504747319387325E-5 5.500949463877465E-5 8 0.0 0.0 0.0 2.7504747319387325E-5 8.251424195816198E-5 9 0.0 0.0 0.0 2.7504747319387325E-5 8.251424195816198E-5 10-11 0.0 0.0 0.0 5.500949463877465E-5 8.251424195816198E-5 12-13 8.251424195816198E-5 0.0 0.0 5.500949463877465E-5 8.251424195816198E-5 14-15 8.251424195816198E-5 0.0 0.0 8.251424195816198E-5 9.626661561785565E-5 16-17 9.626661561785565E-5 0.0 0.0 1.3752373659693662E-4 1.100189892775493E-4 18-19 1.3752373659693662E-4 0.0 0.0 1.925332312357113E-4 1.3752373659693662E-4 20-21 1.3752373659693662E-4 0.0 0.0 2.3379035221479227E-4 1.512761102566303E-4 22-23 1.3752373659693662E-4 0.0 0.0 3.5756171515203525E-4 1.925332312357113E-4 24-25 2.4754272587448596E-4 0.0 0.0 4.6758070442958453E-4 2.200379785550986E-4 26-27 2.612950995341796E-4 0.0 0.0 8.388947932413135E-4 2.4754272587448596E-4 28-29 2.8879984685356694E-4 0.0 0.0 0.002970512710493831 2.7504747319387324E-4 30-31 3.300569678326479E-4 0.0 0.0 0.008622738284627927 3.025522205132606E-4 32-33 3.438093414923416E-4 0.0 0.0 0.01963838958604255 3.300569678326479E-4 34-35 4.2632358345050356E-4 0.0 0.0 0.03542611454737088 3.5756171515203525E-4 36-37 4.6758070442958453E-4 0.0 0.0 0.0618444243476424 3.5756171515203525E-4 38-39 4.950854517489719E-4 2.7504747319387325E-5 0.0 0.10427049708779736 3.5756171515203525E-4 40-41 4.950854517489719E-4 2.7504747319387325E-5 0.0 0.14885569249252423 3.5756171515203525E-4 42-43 5.088378254086655E-4 2.7504747319387325E-5 0.0 0.19741532388490252 3.713140888117289E-4 44-45 5.775996937071339E-4 2.7504747319387325E-5 0.0 0.25813205359245006 3.988188361311163E-4 46-47 6.738663093249894E-4 2.7504747319387325E-5 0.0 0.33022199631656424 4.125712097908099E-4 48-49 7.426281776234578E-4 2.7504747319387325E-5 0.0 0.4104258394998977 4.400759571101972E-4 50-51 7.426281776234578E-4 2.7504747319387325E-5 0.0 0.49454910917624384 4.400759571101972E-4 52-53 7.426281776234578E-4 2.7504747319387325E-5 0.0 0.5847371756365148 5.225901990683592E-4 54-55 7.426281776234578E-4 2.7504747319387325E-5 0.0 0.685198265220577 5.500949463877465E-4 56-57 7.426281776234578E-4 2.7504747319387325E-5 0.0 0.8094922183568884 5.500949463877465E-4 58-59 7.426281776234578E-4 2.7504747319387325E-5 0.0 0.9482949257041766 5.500949463877465E-4 60-61 7.426281776234578E-4 2.7504747319387325E-5 0.0 1.0995985407081261 5.775996937071339E-4 62-63 7.426281776234578E-4 2.7504747319387325E-5 0.0 1.2566093907808487 6.326091883459085E-4 64-65 7.426281776234578E-4 2.7504747319387325E-5 0.0 1.4228068264582467 6.326091883459085E-4 66-67 7.426281776234578E-4 2.7504747319387325E-5 0.0 1.6164952570813722 6.326091883459085E-4 68-69 7.701329249428452E-4 2.7504747319387325E-5 0.0 1.8390224152688752 6.326091883459085E-4 70-71 8.113900459219261E-4 2.7504747319387325E-5 0.0 2.0877065881571157 6.326091883459085E-4 72-73 8.251424195816198E-4 2.7504747319387325E-5 0.0 2.34431212827334 7.013710566443768E-4 74-75 8.251424195816198E-4 2.7504747319387325E-5 0.0 2.6123321385271097 7.151234303040705E-4 76-77 8.251424195816198E-4 2.7504747319387325E-5 0.0 2.907801886605628 7.151234303040705E-4 78-79 8.251424195816198E-4 2.7504747319387325E-5 0.0 3.237515045096784 7.151234303040705E-4 80-81 9.626661561785565E-4 2.7504747319387325E-5 0.0 3.6038920317646825 7.426281776234578E-4 82-83 9.626661561785565E-4 2.7504747319387325E-5 0.0 3.984557734665003 7.976376722622324E-4 84-85 9.764185298382502E-4 2.7504747319387325E-5 0.0 4.3749188609954075 7.976376722622324E-4 86-87 0.001017675650817331 2.7504747319387325E-5 0.0 4.78828770845848 8.526471669010071E-4 88-89 0.0010451803981367184 2.7504747319387325E-5 0.0 5.236546327896194 8.801519142203944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11855 0.0 30.179234 1 GTATCAA 16700 0.0 26.102041 1 GTACATG 12670 0.0 25.004313 1 TACATGG 12790 0.0 24.081327 2 ACATGGG 13240 0.0 22.438438 3 ATCAACG 19615 0.0 21.944479 3 TCAACGC 19800 0.0 21.739443 4 CAACGCA 19920 0.0 21.65613 5 TATCAAC 20095 0.0 21.63285 2 AACGCAG 20430 0.0 21.16198 6 GAGTACT 10255 0.0 19.715063 12-13 ACGCAGA 22585 0.0 18.91162 7 GTATAGG 915 0.0 18.743525 1 GTACTTT 10745 0.0 18.484735 14-15 CGCAGAG 22760 0.0 18.474289 8 AGTACTT 10425 0.0 18.43755 12-13 CATGGGG 7400 0.0 18.341743 4 CATGGGA 7375 0.0 17.438679 4 GCAGAGT 24050 0.0 16.891375 9 AGAGTAC 19390 0.0 16.851957 10-11 >>END_MODULE