FastQCFastQC Report
Fri 27 May 2016
SRR939014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643035
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93360.2562698409430598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76390.20968780151714161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.12862901399519905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.030.931961
GTATCAA163750.028.4048481
ATCAACG187500.024.3869823
TCAACGC188500.024.2576074
CAACGCA188900.024.2065735
TATCAAC191400.023.964262
AACGCAG194400.023.6415166
GTACATG122950.023.0609631
TACATGG123400.022.516722
ACGCAGA214050.021.1395157
GAGTACT98300.020.89765212-13
ACATGGG127750.020.8236833
CGCAGAG214650.020.8158178
GTACTTT102450.019.98211314-15
AGTACTT99700.019.2986412-13
GCAGAGT226150.018.7741439
CATGGGG72800.017.6855964
CAGAGTA216000.016.74186710-11
AGAGTAC186500.016.72518210-11
ACTTTTT127200.016.42989316-17
Produced by FastQC (version 0.11.5)