##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939014_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3643035 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738145255261067 33.0 31.0 34.0 30.0 34.0 2 31.813530476649277 33.0 31.0 34.0 30.0 34.0 3 31.865032864081734 34.0 31.0 34.0 30.0 34.0 4 35.397827635474265 37.0 35.0 37.0 33.0 37.0 5 35.423049737375564 37.0 35.0 37.0 33.0 37.0 6 35.48988521932949 37.0 35.0 37.0 33.0 37.0 7 35.395455712064255 37.0 35.0 37.0 33.0 37.0 8 35.37506886428486 37.0 35.0 37.0 33.0 37.0 9 36.98188515894028 39.0 37.0 39.0 33.0 39.0 10-11 36.8629005760307 39.0 37.0 39.0 33.0 39.0 12-13 36.735277316852574 39.0 37.0 39.0 32.0 39.0 14-15 37.95355452253409 40.0 38.0 41.0 33.0 41.0 16-17 37.86996830939039 40.0 38.0 41.0 32.5 41.0 18-19 37.78356315544594 40.0 38.0 41.0 32.0 41.0 20-21 37.65729632023849 40.0 37.5 41.0 32.0 41.0 22-23 37.74172990377528 40.0 38.0 41.0 32.0 41.0 24-25 37.68511213864264 40.0 38.0 41.0 32.0 41.0 26-27 37.60370748565413 40.0 37.0 41.0 32.0 41.0 28-29 37.466990984165676 40.0 37.0 41.0 31.5 41.0 30-31 37.28891473730008 40.0 37.0 41.0 31.0 41.0 32-33 37.113596630282174 40.0 36.5 41.0 30.5 41.0 34-35 36.8007670801955 40.0 36.0 41.0 30.0 41.0 36-37 36.54837601066144 39.5 36.0 41.0 30.0 41.0 38-39 36.344427105421715 39.0 35.5 41.0 30.0 41.0 40-41 36.19061743848192 39.0 35.0 41.0 29.5 41.0 42-43 35.99006666145124 39.0 35.0 41.0 28.5 41.0 44-45 35.952296368275356 39.0 35.0 41.0 28.0 41.0 46-47 35.75042485180626 39.0 35.0 41.0 27.0 41.0 48-49 35.59019018483215 39.0 35.0 41.0 26.5 41.0 50-51 34.43969643442899 38.0 34.0 40.0 25.0 40.5 52-53 34.58369161976209 38.0 34.0 39.5 25.0 40.5 54-55 35.074183064395484 39.0 35.0 40.0 26.0 41.0 56-57 34.96981829710667 39.0 35.0 41.0 25.5 41.0 58-59 34.75608894781411 38.0 34.0 40.0 25.0 41.0 60-61 34.48492740256407 38.0 34.0 40.0 24.5 41.0 62-63 34.11946838281817 37.5 34.0 40.0 23.5 41.0 64-65 33.697284269846435 37.0 33.5 40.0 22.0 41.0 66-67 33.32126880471914 36.5 33.0 39.0 22.0 41.0 68-69 32.92653542993685 36.0 32.5 39.0 20.5 41.0 70-71 32.51553635910717 35.0 32.0 39.0 20.0 40.0 72-73 32.07496318317008 35.0 32.0 38.0 18.5 40.0 74-75 31.605465635109187 35.0 31.0 37.0 17.5 39.0 76-77 31.190562264705115 35.0 31.0 37.0 14.0 39.0 78-79 30.763219266353467 35.0 31.0 36.0 9.5 39.0 80-81 30.3281443905974 34.0 30.0 36.0 7.0 37.5 82-83 29.945570520184404 34.0 30.0 35.0 4.0 37.0 84-85 29.59294901092084 34.0 30.0 35.0 2.0 36.5 86-87 29.192545363961642 34.0 29.0 35.0 2.0 36.0 88-89 28.78206124838219 34.0 29.0 35.0 2.0 36.0 90-91 28.357684458150963 34.0 29.0 35.0 2.0 35.5 92-93 27.92897254624235 34.0 27.5 35.0 2.0 35.0 94-95 27.447843624889686 34.0 27.0 35.0 2.0 35.0 96-97 26.856531985007006 33.0 25.5 35.0 2.0 35.0 98-99 26.118041276023973 33.0 24.5 35.0 2.0 35.0 100-101 23.472473500803588 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18845006245582496 1101 2 0.24828038350419135 1101 3 0.29570881040408636 1101 4 0.21353608992376394 1101 5 0.05276638043320503 1101 6 0.12372442139180606 1101 7 0.12451352020628548 1101 8 0.1560941712056021 1101 9 0.1274024063717647 1101 10-11 0.2711840327602246 1101 12-13 0.2813677253428324 1101 14-15 0.07116762954267841 1101 16-17 0.2899883618071186 1101 18-19 0.30147187285237464 1101 20-21 0.46728824140298997 1101 22-23 0.3707907498691725 1101 24-25 0.41149303896015965 1101 26-27 0.4616537339740674 1101 28-29 0.4986942032937449 1101 30-31 0.5024046164219769 1101 32-33 0.4344341532455047 1101 34-35 0.41986004671633737 1101 36-37 0.34957269056024387 1101 38-39 0.34925794126095155 1101 40-41 0.2353521015904576 1101 42-43 0.16243656685320929 1101 44-45 0.2821629833082895 1101 46-47 0.4306906023768633 1101 48-49 0.40914081532742586 1101 50-51 0.4083346390909668 1101 52-53 0.33123768445919666 1101 54-55 0.363263035285442 1101 56-57 0.25463073086877586 1101 58-59 0.23911460621510372 1101 60-61 0.19391582851525158 1101 62-63 0.18241272895058103 1101 64-65 0.1852100143798765 1101 66-67 0.1999160981185426 1101 68-69 0.19521640967313658 1101 70-71 0.11344092113138515 1101 72-73 0.21451987050371457 1101 74-75 0.15222507859301615 1101 76-77 -0.036342596616908196 1101 78-79 -0.015675408242668 1101 80-81 -0.13845766491621347 1101 82-83 -0.0447998859533989 1101 84-85 -0.20699898547236373 1101 86-87 -0.26491469158701975 1101 88-89 -0.27755018035659873 1101 90-91 -0.28094187974008733 1101 92-93 -0.27486908326072523 1101 94-95 -0.32080016248173493 1101 96-97 -0.34215577047066503 1101 98-99 -0.5429184991217149 1101 100-101 0.08274664016064648 1102 1 0.22480029063369855 1102 2 0.15793091979994855 1102 3 0.19655931705906937 1102 4 0.2567019115281184 1102 5 0.2727075509752339 1102 6 0.2674703625688082 1102 7 0.42946439623221977 1102 8 0.23087228604898513 1102 9 0.3866705443923948 1102 10-11 0.3294216100212637 1102 12-13 0.3182953877480159 1102 14-15 0.40952777072386226 1102 16-17 0.42259394389225946 1102 18-19 0.4382265684251436 1102 20-21 0.5011782343616034 1102 22-23 0.4813356676236751 1102 24-25 0.47771650184213854 1102 26-27 0.46455165498989714 1102 28-29 0.5367543846021405 1102 30-31 0.4105354682016227 1102 32-33 0.3457493912036469 1102 34-35 0.43295119022646844 1102 36-37 0.3915646508402446 1102 38-39 0.44933345385174306 1102 40-41 0.3049275722864664 1102 42-43 0.27868248975966736 1102 44-45 0.4290270693127667 1102 46-47 0.5048917078893496 1102 48-49 0.43578840641119143 1102 50-51 0.4156583661395459 1102 52-53 0.32194056850309494 1102 54-55 0.39164080456906447 1102 56-57 0.4444086324141594 1102 58-59 0.41124800431398256 1102 60-61 0.28888009217741484 1102 62-63 0.22979496763461071 1102 64-65 0.21161902612691108 1102 66-67 0.1839805280511655 1102 68-69 0.1408791265593834 1102 70-71 0.20433471963225003 1102 72-73 0.1478638205821241 1102 74-75 0.12464955622961327 1102 76-77 0.09435320355396826 1102 78-79 0.02929524586156873 1102 80-81 -0.06373661366129824 1102 82-83 -0.06327055798826464 1102 84-85 -0.03861848495458631 1102 86-87 0.08762139614015396 1102 88-89 0.0820778959146864 1102 90-91 0.05036243158185627 1102 92-93 0.2019902082126066 1102 94-95 0.06973844811038887 1102 96-97 -0.20379619865067866 1102 98-99 -0.127291497328482 1102 100-101 -0.010686296823646302 1103 1 0.12998210864965287 1103 2 0.27759972849415604 1103 3 0.313472704186637 1103 4 0.3245065127047937 1103 5 0.33268791595310887 1103 6 0.2460810761122758 1103 7 0.31521503941272044 1103 8 0.21016486794823663 1103 9 0.3446724461930728 1103 10-11 0.42241528412069584 1103 12-13 0.4208048656518173 1103 14-15 0.45566144777236417 1103 16-17 0.5639420528644763 1103 18-19 0.6128904401668862 1103 20-21 0.6317697912357474 1103 22-23 0.6594796637570468 1103 24-25 0.66602317275715 1103 26-27 0.5753996729855189 1103 28-29 0.5009619353114374 1103 30-31 0.5991827000997247 1103 32-33 0.48350243178749963 1103 34-35 0.4133403013643715 1103 36-37 0.4655462478585335 1103 38-39 0.4618117608676542 1103 40-41 0.4778044629534932 1103 42-43 0.49952594150963847 1103 44-45 0.530620395461959 1103 46-47 0.5927332205191718 1103 48-49 0.4636699968446649 1103 50-51 0.4373376249514678 1103 52-53 0.5346579109315428 1103 54-55 0.7324808959072513 1103 56-57 0.7235082062588774 1103 58-59 0.6926690156453432 1103 60-61 0.5912485687946045 1103 62-63 0.5598860122366958 1103 64-65 0.45774737892967465 1103 66-67 0.49292735077850125 1103 68-69 0.4205236062702795 1103 70-71 0.42594504521899523 1103 72-73 0.4095339950034145 1103 74-75 0.3055143497459092 1103 76-77 0.2541309569312524 1103 78-79 0.31522229407485014 1103 80-81 0.41079213668514925 1103 82-83 0.44990661847963054 1103 84-85 0.2970932049825734 1103 86-87 0.3883473595342224 1103 88-89 0.2955362788069067 1103 90-91 0.2376499075128642 1103 92-93 0.14015697254573212 1103 94-95 0.2876000319855869 1103 96-97 0.2497183674738821 1103 98-99 0.24090717267161565 1103 100-101 0.49218871294161914 1104 1 0.17509633037010985 1104 2 0.21321773824466206 1104 3 0.1719788009728873 1104 4 0.1956708373391507 1104 5 0.19907681795076826 1104 6 0.18288522751731762 1104 7 0.23552392630907093 1104 8 0.23711487756806093 1104 9 0.37110516373707725 1104 10-11 0.23222150832415878 1104 12-13 0.24893911111325906 1104 14-15 0.2626003310748999 1104 16-17 0.15059437450761948 1104 18-19 0.2481741158810209 1104 20-21 0.29160033724206613 1104 22-23 0.1928291208726236 1104 24-25 0.3329678461008143 1104 26-27 0.2088424241807303 1104 28-29 0.27745863371745116 1104 30-31 0.32121988704726334 1104 32-33 0.3672982121362125 1104 34-35 0.29704986636711794 1104 36-37 0.221913931805787 1104 38-39 0.16576051446600815 1104 40-41 0.2745100384545367 1104 42-43 0.17884478582469399 1104 44-45 0.15929180790528363 1104 46-47 0.219967105509717 1104 48-49 0.22565801381910688 1104 50-51 0.2741483105722011 1104 52-53 0.20665661860888918 1104 54-55 0.31445308555923646 1104 56-57 0.4105356100334774 1104 58-59 0.3360641941201905 1104 60-61 0.2689796894488907 1104 62-63 0.15755075614989522 1104 64-65 0.15502397228001996 1104 66-67 0.1999308502140167 1104 68-69 0.22370486178352422 1104 70-71 0.2207883692471242 1104 72-73 0.08135346551414102 1104 74-75 0.13274605666455486 1104 76-77 0.08275753739467362 1104 78-79 0.0903195812863018 1104 80-81 0.061846414236853775 1104 82-83 -0.004432826765455644 1104 84-85 0.07632020726591904 1104 86-87 0.16169946663727686 1104 88-89 0.23212579937698052 1104 90-91 0.19126741267670866 1104 92-93 0.20950950437280724 1104 94-95 0.20744925495843702 1104 96-97 0.17993158256211217 1104 98-99 0.2255948734626152 1104 100-101 0.581953408574222 1105 1 0.10182621042277518 1105 2 0.15368661621353752 1105 3 0.2054191746031364 1105 4 0.13214708631539906 1105 5 0.19287146127072674 1105 6 0.21420326160433945 1105 7 0.17860596116326377 1105 8 0.22214057501464168 1105 9 0.24137063922407265 1105 10-11 0.18888185106045086 1105 12-13 0.3249778944305248 1105 14-15 0.31121603178711865 1105 16-17 0.29613580921044047 1105 18-19 0.26391947142067806 1105 20-21 0.262512687307904 1105 22-23 0.2603837295664704 1105 24-25 0.24896032736228335 1105 26-27 0.14497493903160574 1105 28-29 0.21362921525987133 1105 30-31 0.28165142402152554 1105 32-33 0.2628375410220585 1105 34-35 0.16731145419198867 1105 36-37 0.21786979129011996 1105 38-39 0.19050030028174092 1105 40-41 0.08031629422124098 1105 42-43 0.10992981936225732 1105 44-45 0.13505531234758195 1105 46-47 0.161510760067614 1105 48-49 0.2549381030279889 1105 50-51 0.20462605889013474 1105 52-53 0.25199032256600873 1105 54-55 0.2811142771017501 1105 56-57 0.1901730401851438 1105 58-59 0.04848119253401961 1105 60-61 0.2925034469520824 1105 62-63 0.15640340986805512 1105 64-65 0.2313403994667027 1105 66-67 0.12612066920446807 1105 68-69 0.08444475515791083 1105 70-71 -0.06258640008492478 1105 72-73 0.20501691967600522 1105 74-75 0.11117487745888255 1105 76-77 0.07159897520763181 1105 78-79 -0.012991799714953345 1105 80-81 -0.04419361961172541 1105 82-83 -0.0936935489375692 1105 84-85 -0.07977639206644227 1105 86-87 0.09388649039866692 1105 88-89 0.06808346662420206 1105 90-91 0.08744415616358125 1105 92-93 0.19496576039868074 1105 94-95 0.25082859877290886 1105 96-97 0.09757939795517956 1105 98-99 0.11653833778424527 1105 100-101 0.21216886928810297 1106 1 0.1602807298213058 1106 2 0.21110856650503607 1106 3 0.18758608032519675 1106 4 0.206374568173473 1106 5 0.20508691025680292 1106 6 0.230569247276037 1106 7 0.1603413781584706 1106 8 0.16098549627655245 1106 9 0.2542287842543445 1106 10-11 0.13734163343243466 1106 12-13 0.24483175774090427 1106 14-15 0.3107350657905599 1106 16-17 0.32235250416324845 1106 18-19 0.21519011028683366 1106 20-21 0.3197778357224976 1106 22-23 0.33241043117778446 1106 24-25 0.2687520957213678 1106 26-27 0.34300268510221343 1106 28-29 0.29130817669560116 1106 30-31 0.4261108537555103 1106 32-33 0.36762926401067375 1106 34-35 0.33116499584144776 1106 36-37 0.21479437807474966 1106 38-39 0.25648459521853084 1106 40-41 0.23387203198873152 1106 42-43 0.16035400188042814 1106 44-45 0.18047704955436927 1106 46-47 0.25196486988040334 1106 48-49 0.3335182156844141 1106 50-51 0.27704204687729117 1106 52-53 0.14662939585713275 1106 54-55 0.016707395748326803 1106 56-57 0.17931631916153634 1106 58-59 0.3093942746213898 1106 60-61 0.26569455014891474 1106 62-63 0.20401552340559448 1106 64-65 -0.020507821009957183 1106 66-67 0.22544278758569192 1106 68-69 0.1834097376104893 1106 70-71 0.2216573854135504 1106 72-73 0.04040666914345792 1106 74-75 0.1003252749041188 1106 76-77 0.05087320115628202 1106 78-79 0.13403117838957712 1106 80-81 0.06647318692111881 1106 82-83 0.17899293304315833 1106 84-85 0.15434085678146303 1106 86-87 0.3821144281516453 1106 88-89 0.36693137764303074 1106 90-91 0.35032670737274785 1106 92-93 0.3127358992510665 1106 94-95 0.36193025770469234 1106 96-97 0.45214411219011197 1106 98-99 0.46902007852053984 1106 100-101 0.8426402730689304 1107 1 0.15147595415146853 1107 2 0.1067773949622115 1107 3 0.13539144010172066 1107 4 0.10695789336304529 1107 5 0.06590850207092558 1107 6 0.08323841517896824 1107 7 0.012910190204330263 1107 8 0.10291350019546286 1107 9 0.11752545945783766 1107 10-11 0.1266711441129047 1107 12-13 0.18443509599298835 1107 14-15 0.16855139816853892 1107 16-17 0.15982162848836623 1107 18-19 0.19953154025643727 1107 20-21 0.21579597529513705 1107 22-23 0.11727915883032125 1107 24-25 0.19229675589608064 1107 26-27 0.21917860698527392 1107 28-29 0.17710526179512698 1107 30-31 0.2919162706350491 1107 32-33 0.3185392718882696 1107 34-35 0.23185014702015394 1107 36-37 0.22494618159019808 1107 38-39 0.3576298358323342 1107 40-41 0.286575067091853 1107 42-43 0.3178348578594026 1107 44-45 0.3840106821911107 1107 46-47 0.37877513440435706 1107 48-49 0.3533761792621206 1107 50-51 0.2620531993764885 1107 52-53 0.22926764688280343 1107 54-55 0.4326460955743556 1107 56-57 0.4520934427305505 1107 58-59 0.43787975462995377 1107 60-61 0.3024075004416602 1107 62-63 0.21068736957964518 1107 64-65 0.40953903983639606 1107 66-67 0.37242171649235445 1107 68-69 0.2941790430171949 1107 70-71 0.3806033827430184 1107 72-73 0.3044625824440388 1107 74-75 0.3308916572085572 1107 76-77 0.34486730933123155 1107 78-79 0.40149176279492593 1107 80-81 0.38601490187969034 1107 82-83 0.43874302297268386 1107 84-85 0.455426456458774 1107 86-87 0.5673838364289097 1107 88-89 0.6080509482035268 1107 90-91 0.6639798083571264 1107 92-93 0.5331921446776988 1107 94-95 0.5576141201965861 1107 96-97 0.7277366934085308 1107 98-99 0.7718420202260852 1107 100-101 0.81543884509097 1108 1 0.16992997522137188 1108 2 0.27653910872308884 1108 3 0.1934219814429241 1108 4 0.20998107978060432 1108 5 0.22008287106165625 1108 6 0.21320658721054997 1108 7 0.24770360183147488 1108 8 0.2017462901447118 1108 9 0.28758834585430293 1108 10-11 0.31620478603746704 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-2.1788309734926834 2316 92-93 -2.4128318264071957 2316 94-95 -2.673889733502005 2316 96-97 -2.750703609914474 2316 98-99 -2.993912591289263 2316 100-101 -3.487588842380031 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13096.0 3 3656.0 4 3604.0 5 4057.0 6 4654.0 7 5567.0 8 6930.0 9 8434.0 10 10283.0 11 12775.0 12 15755.0 13 22194.0 14 21137.0 15 19394.0 16 23155.0 17 29404.0 18 26208.0 19 20779.0 20 21326.0 21 23214.0 22 25306.0 23 28281.0 24 31995.0 25 36985.0 26 42870.0 27 49906.0 28 59444.0 29 70692.0 30 84398.0 31 102121.0 32 127330.0 33 163163.0 34 218646.0 35 311504.0 36 481837.0 37 740580.0 38 667906.0 39 104409.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.522776634769485 18.771007021196827 14.06127760126451 23.64493874276918 2 15.25473973525563 20.009407076682237 40.62821359229169 24.10763959577044 3 19.334499191546474 23.909041079330105 29.597092040064126 27.159367689059295 4 12.954221142244288 16.25753022187208 36.68623248879071 34.102016147092925 5 14.16473876695574 36.95005395554459 34.11861756194583 14.766589715553843 6 32.74439608978344 35.28859728961762 17.391606545379222 14.575400075219719 7 28.96969706960673 32.24449177502879 20.69543538276229 18.090375772602187 8 25.94731713106757 35.67961131706073 19.71094948347139 18.662122068400308 9 26.32481309605256 16.996018760457964 20.394716958321997 36.28445118516748 10-11 25.194644384386912 26.36608227332882 27.13779138276019 21.30148195952408 12-13 25.95581929163545 23.641004922018247 27.760504365030247 22.642671421316052 14-15 22.8852753523711 25.007161385971706 25.91187971234653 26.19568354931066 16-17 22.570864328864555 28.276853605115924 26.592187642522664 22.560094423496857 18-19 22.492837680047185 27.067407621425588 28.463615222298106 21.976139476229122 20-21 23.15397104079187 26.34444094406837 28.37013742922214 22.131450585917616 22-23 22.94649494409581 26.262066084700653 28.24610484457165 22.545334126631882 24-25 22.736349495569424 26.32102021501428 28.377550685265085 22.565079604151208 26-27 22.663358363200597 26.49717452010818 28.303763825062116 22.5357032916291 28-29 22.512309401976953 26.60768395807101 28.459999716922574 22.42000692302946 30-31 22.649897199255093 26.451456791602695 28.319320837669082 22.579325171473126 32-33 22.501466401777737 26.580254874300273 28.248799570322642 22.66947915359935 34-35 22.852808048949953 26.59899046442577 28.080737495262248 22.46746399136203 36-37 22.845157195837267 26.43159989270257 28.13314489556517 22.590098015894984 38-39 22.698442802596443 26.50145306584885 28.301380240016055 22.49872389153865 40-41 22.796608497356367 26.48692146032367 28.186398252506034 22.53007178981393 42-43 22.633053406857854 26.59282194650067 27.95719093050365 22.81693371613783 44-45 22.69271880840704 26.657954028247005 27.930848689853217 22.71847847349274 46-47 22.78675583638991 26.80073228088546 27.597142265976753 22.815369616747883 48-49 22.919508453291247 26.788765467142778 27.516133984693838 22.775592094872138 50-51 22.795956472370722 27.01739103424712 27.527684497164767 22.658967996217388 52-53 22.918530706280553 27.006788060887537 27.35394293301827 22.72073829981364 54-55 22.767186102116575 26.79284189817221 27.44481075992955 22.99516123978167 56-57 22.71429346221792 27.181879317537682 27.34988524287234 22.753941977372055 58-59 22.852596049777762 26.81254708288617 27.46864050361214 22.86621636372393 60-61 22.815665274616485 26.717681382014952 27.723411089811307 22.74324225355726 62-63 22.802491979988414 26.767073356225435 27.72075149185604 22.709683171930113 64-65 22.658432025131958 26.747898352095962 27.602351260878283 22.9913183618938 66-67 22.528474842159337 26.937877339991275 27.463115274778083 23.070532543071305 68-69 22.464971349296636 26.863580008059262 27.707620587188202 22.963828055455895 70-71 22.608342021500626 26.703071813744106 27.548473068982897 23.14011309577237 72-73 22.587637298756906 26.719565614818407 27.438100608707032 23.254696477717655 74-75 22.541455947140516 26.94667976168828 27.320365616759084 23.191498674412113 76-77 22.669317060624934 26.672656236734166 27.43182595973358 23.22620074290732 78-79 22.556273194128995 26.76429961873572 27.484815126022582 23.1946120611127 80-81 22.53309190378333 26.904866728404176 27.421101807547345 23.14093956026515 82-83 22.6112600314159 26.81361926031852 27.383053084030625 23.192067624234962 84-85 22.612455018268783 26.63565915871394 27.30495308426831 23.446932738748977 86-87 22.527526981756342 26.53707325759071 27.627248019589466 23.30815174106348 88-89 22.620293518795002 26.46128164637127 27.524776148743722 23.393648686090003 90-91 22.500210211109945 26.50601245195347 27.636347620279068 23.357429716657517 92-93 22.503906995420415 26.5021599975035 27.506628567420282 23.487304439655805 94-95 22.465705039869256 26.35317139664731 27.523124278186156 23.657999285297286 96-97 22.438076796632757 26.176451460887908 27.650297639799852 23.735174102679483 98-99 22.319546354018012 26.222780253801268 27.50578801271395 23.951885379466766 100-101 22.56104968516403 25.703459468354335 27.969544044180296 23.765946802301343 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3561.0 1 2013.5 2 516.0 3 577.0 4 534.5 5 544.0 6 845.5 7 1613.5 8 2598.0 9 3177.5 10 3210.0 11 3299.0 12 3593.0 13 3943.0 14 4319.5 15 4633.0 16 4950.0 17 5211.5 18 5541.0 19 5769.5 20 6060.5 21 6853.5 22 8063.0 23 9719.5 24 11778.0 25 14601.5 26 18478.0 27 22955.0 28 27356.5 29 32688.5 30 38532.5 31 44701.5 32 50285.0 33 55482.5 34 62515.0 35 69785.0 36 77904.0 37 86089.0 38 91773.5 39 95793.5 40 99428.0 41 105014.0 42 111324.0 43 117341.0 44 124012.0 45 129063.5 46 132631.0 47 137598.0 48 149906.5 49 153091.0 50 144873.5 51 141432.0 52 137752.0 53 135012.0 54 131463.5 55 122975.5 56 111868.0 57 100596.0 58 90947.5 59 80194.5 60 67814.0 61 54911.5 62 43533.0 63 34695.5 64 26178.5 65 18814.0 66 13687.0 67 10645.5 68 8087.0 69 5636.0 70 3849.0 71 2675.0 72 1867.5 73 1286.5 74 946.0 75 612.5 76 375.5 77 210.0 78 131.5 79 97.5 80 69.5 81 50.5 82 44.0 83 34.0 84 24.5 85 25.5 86 26.0 87 20.5 88 20.0 89 17.5 90 10.0 91 7.0 92 7.0 93 4.0 94 3.0 95 1.5 96 1.0 97 2.0 98 3.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2833077365438432 2 0.0884427407367758 3 0.09250528748694427 4 0.10612030902804942 5 0.10782218672068757 6 0.083227309098046 7 0.09297193137040957 8 0.08937602850370639 9 0.09511300330630916 10-11 0.08761925153066055 12-13 0.09249156266684234 14-15 0.10226363457940975 16-17 0.08956817598513328 18-19 0.10091860220942153 20-21 0.1027577281030789 22-23 0.11583748166020914 24-25 0.1269683107628667 26-27 0.12942505356111045 28-29 0.12227442228800985 30-31 0.1152747640360304 32-33 0.11049852664056206 34-35 0.38266170926164583 36-37 0.5854047518072156 38-39 0.5680428543782862 40-41 0.5306427196005529 42-43 0.5571727968575653 44-45 0.5790089856397207 46-47 0.5669585935902345 48-49 0.5898104190599321 50-51 0.5818637482209202 52-53 0.5849106582835465 54-55 0.5745072446462908 56-57 0.5475791476063228 58-59 0.5426931116500391 60-61 0.4887820182896953 62-63 0.5636509119456716 64-65 0.5991981960096459 66-67 0.6119897283446357 68-69 0.61366415639707 70-71 0.6096565089273093 72-73 0.6016137643475838 74-75 0.5987178273060786 76-77 0.5814794532580664 78-79 0.5586276277883687 80-81 0.5536592429114735 82-83 0.5672468148123748 84-85 0.5766894910424962 86-87 0.5676585594154324 88-89 0.580683413692155 90-91 0.5949846762383563 92-93 0.602958796717572 94-95 0.6024921528341067 96-97 0.5982786330628171 98-99 0.6031646690191008 100-101 0.5805324406710338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3643035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.1297771235537 #Duplication Level Percentage of deduplicated Percentage of total 1 72.83718806455492 37.24149192047059 2 14.048533550771335 14.365967787274094 3 5.186458280226714 7.955473677858044 4 2.5692121336891938 5.2545297511463325 5 1.3886688899966393 3.5501165421970433 6 0.9029949195729156 2.7701957388878684 7 0.5904323574283059 2.113207238929057 8 0.4213438837429916 1.7234575094521345 9 0.3088723210643575 1.4213315642089785 >10 1.6017510976635378 14.626398855948874 >50 0.09743382005625215 3.399580924009139 >100 0.044315470939760085 3.9520560866714565 >500 0.0023114239054469886 0.7532353544160899 >1k 3.762783014618222E-4 0.406410675543683 >5k 1.0750808613194922E-4 0.4665463729866788 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9336 0.2562698409430598 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7639 0.20968780151714161 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4686 0.12862901399519905 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.744964020384103E-5 2 0.0 0.0 0.0 0.0 2.744964020384103E-5 3 0.0 0.0 0.0 0.0 2.744964020384103E-5 4 0.0 0.0 0.0 2.744964020384103E-5 2.744964020384103E-5 5 0.0 0.0 0.0 2.744964020384103E-5 2.744964020384103E-5 6 0.0 0.0 0.0 2.744964020384103E-5 1.0979856081536411E-4 7 0.0 0.0 0.0 2.744964020384103E-5 1.0979856081536411E-4 8 0.0 0.0 0.0 2.744964020384103E-5 1.0979856081536411E-4 9 0.0 0.0 0.0 2.744964020384103E-5 1.0979856081536411E-4 10-11 0.0 0.0 0.0 4.117446030576154E-5 1.0979856081536411E-4 12-13 0.0 0.0 0.0 9.60737407134436E-5 1.7842266132496668E-4 14-15 0.0 0.0 0.0 1.2352338091728462E-4 2.4704676183456923E-4 16-17 0.0 0.0 0.0 1.3724820101920513E-4 3.019460422422513E-4 18-19 0.0 0.0 0.0 2.0587230152880768E-4 3.019460422422513E-4 20-21 0.0 0.0 0.0 2.6077158193648975E-4 3.705701427518539E-4 22-23 0.0 0.0 0.0 3.980197829556949E-4 4.117446030576154E-4 24-25 1.3724820101920513E-4 0.0 0.0 4.666438834652975E-4 4.117446030576154E-4 26-27 1.3724820101920513E-4 0.0 0.0 6.725161849941052E-4 4.117446030576154E-4 28-29 2.1959712163072823E-4 0.0 0.0 0.0028135881208937053 4.117446030576154E-4 30-31 2.1959712163072823E-4 0.0 0.0 0.008454489182783038 4.117446030576154E-4 32-33 2.1959712163072823E-4 0.0 0.0 0.019297097063300243 4.117446030576154E-4 34-35 2.1959712163072823E-4 0.0 0.0 0.035588458524279894 4.666438834652975E-4 36-37 2.1959712163072823E-4 0.0 0.0 0.06346356815128046 4.666438834652975E-4 38-39 2.1959712163072823E-4 1.3724820101920514E-5 0.0 0.10428118313439207 4.666438834652975E-4 40-41 2.1959712163072823E-4 2.744964020384103E-5 0.0 0.1512063430628583 4.666438834652975E-4 42-43 2.4704676183456923E-4 2.744964020384103E-5 0.0 0.19836482493305718 4.666438834652975E-4 44-45 3.019460422422513E-4 2.744964020384103E-5 0.0 0.2564894380646906 4.666438834652975E-4 46-47 3.019460422422513E-4 2.744964020384103E-5 0.0 0.3295054810069077 4.940935236691385E-4 48-49 3.019460422422513E-4 4.117446030576154E-5 0.0 0.4102211480263023 5.764424442806616E-4 50-51 3.019460422422513E-4 5.489928040768206E-5 0.0 0.4980050973981858 6.038920844845026E-4 52-53 3.019460422422513E-4 5.489928040768206E-5 0.0 0.5867909586375096 6.176169045864231E-4 54-55 3.019460422422513E-4 5.489928040768206E-5 0.0 0.6886977478942695 6.313417246883436E-4 56-57 3.019460422422513E-4 5.489928040768206E-5 0.0 0.8109309957219736 6.313417246883436E-4 58-59 3.019460422422513E-4 5.489928040768206E-5 0.0 0.9468753388314963 6.313417246883436E-4 60-61 3.019460422422513E-4 5.489928040768206E-5 0.0 1.1027481207290075 6.313417246883436E-4 62-63 3.019460422422513E-4 5.489928040768206E-5 0.0 1.2623540536942413 6.587913648921847E-4 64-65 3.019460422422513E-4 5.489928040768206E-5 0.0 1.4313477636091885 6.862410050960257E-4 66-67 3.5684532264993336E-4 6.862410050960257E-5 0.0 1.6246069554643312 6.862410050960257E-4 68-69 3.842949628537744E-4 8.234892061152308E-5 0.0 1.8519586004526447 6.862410050960257E-4 70-71 3.980197829556949E-4 8.234892061152308E-5 0.0 2.1055109270155246 6.862410050960257E-4 72-73 4.117446030576154E-4 8.234892061152308E-5 0.0 2.364690429820191 7.411402855037077E-4 74-75 4.117446030576154E-4 8.234892061152308E-5 0.0 2.6256953336984137 7.960395659113898E-4 76-77 4.117446030576154E-4 8.234892061152308E-5 0.0 2.9184182968321744 7.960395659113898E-4 78-79 4.117446030576154E-4 8.234892061152308E-5 0.0 3.251025587182116 7.960395659113898E-4 80-81 4.940935236691385E-4 8.234892061152308E-5 0.0 3.62130750871183 7.960395659113898E-4 82-83 4.940935236691385E-4 8.234892061152308E-5 0.0 4.0079219661628285 8.509388463190719E-4 84-85 5.07818343771059E-4 8.234892061152308E-5 0.0 4.400794941580303 8.509388463190719E-4 86-87 5.215431638729795E-4 8.234892061152308E-5 0.0 4.813514006865155 8.646636664209924E-4 88-89 5.215431638729795E-4 8.234892061152308E-5 0.0 5.26205485261602 8.783884865229129E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11435 0.0 30.93196 1 GTATCAA 16375 0.0 28.404848 1 ATCAACG 18750 0.0 24.386982 3 TCAACGC 18850 0.0 24.257607 4 CAACGCA 18890 0.0 24.206573 5 TATCAAC 19140 0.0 23.96426 2 AACGCAG 19440 0.0 23.641516 6 GTACATG 12295 0.0 23.060963 1 TACATGG 12340 0.0 22.51672 2 ACGCAGA 21405 0.0 21.139515 7 GAGTACT 9830 0.0 20.897652 12-13 ACATGGG 12775 0.0 20.823683 3 CGCAGAG 21465 0.0 20.815817 8 GTACTTT 10245 0.0 19.982113 14-15 AGTACTT 9970 0.0 19.29864 12-13 GCAGAGT 22615 0.0 18.774143 9 CATGGGG 7280 0.0 17.685596 4 CAGAGTA 21600 0.0 16.741867 10-11 AGAGTAC 18650 0.0 16.725182 10-11 ACTTTTT 12720 0.0 16.429893 16-17 >>END_MODULE