FastQCFastQC Report
Fri 27 May 2016
SRR939014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643035
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.25476011073184857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82500.22645953168168848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50460.13851088446858184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105750.030.6375641
GTATCAA154500.029.327681
ATCAACG184600.024.0606783
TCAACGC186400.023.7774164
TATCAAC188900.023.638582
CAACGCA187250.023.5934565
GTACATG122900.023.2220541
AACGCAG191750.023.1882486
TACATGG123400.022.1498642
CGCGAAA550.002855741721.62088672-73
ACGCAGA214300.020.4160867
CGCAGAG215850.020.1375758
ACATGGG129650.019.6546573
GAGTACT101750.019.58828712-13
AGTACTT105300.019.53630312-13
GTACTTT107250.018.53951314-15
CATGGGG70550.018.496924
AGAGTAC185950.018.2087210-11
GCAGAGT231850.018.1750499
GGACCGT3953.6561687E-1016.8187876
Produced by FastQC (version 0.11.5)