##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939014_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3643035 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16540274798348 33.0 31.0 34.0 30.0 34.0 2 32.39810268086911 34.0 31.0 34.0 31.0 34.0 3 32.4988958382228 34.0 31.0 34.0 30.0 34.0 4 36.06823074716548 37.0 35.0 37.0 35.0 37.0 5 35.97964746427086 37.0 35.0 37.0 35.0 37.0 6 36.016880430739754 37.0 35.0 37.0 35.0 37.0 7 35.956961434628 37.0 35.0 37.0 35.0 37.0 8 35.91860824834239 37.0 35.0 37.0 35.0 37.0 9 37.60490579969723 39.0 37.0 39.0 35.0 39.0 10-11 37.52408911800188 39.0 37.0 39.0 35.0 39.0 12-13 37.44983962547711 39.0 37.0 39.0 34.0 39.0 14-15 38.72624707146652 40.0 38.0 41.0 34.0 41.0 16-17 38.71104408823961 40.0 38.0 41.0 34.0 41.0 18-19 38.79505124161585 40.0 38.0 41.0 34.0 41.0 20-21 38.793214037197004 40.0 38.0 41.0 34.0 41.0 22-23 38.73371145212714 40.0 38.0 41.0 34.0 41.0 24-25 38.676751252732956 40.0 38.0 41.0 34.0 41.0 26-27 38.53193395067574 40.0 38.0 41.0 34.0 41.0 28-29 38.349249046468124 40.0 38.0 41.0 34.0 41.0 30-31 38.193777715558596 40.0 38.0 41.0 33.5 41.0 32-33 38.05999997255036 40.0 37.5 41.0 33.0 41.0 34-35 38.052408362807384 40.0 38.0 41.0 33.0 41.0 36-37 37.96253453507858 40.0 37.0 41.0 33.0 41.0 38-39 37.84726498647419 40.0 37.0 41.0 33.0 41.0 40-41 37.69551116033746 40.0 37.0 41.0 33.0 41.0 42-43 37.53896558775856 40.0 37.0 41.0 32.0 41.0 44-45 37.3086316766103 40.0 37.0 41.0 31.5 41.0 46-47 37.03030152057282 40.0 36.0 41.0 31.0 41.0 48-49 36.76728167585543 39.5 36.0 41.0 30.5 41.0 50-51 36.511882263003244 39.0 36.0 41.0 30.0 41.0 52-53 36.27885979684521 39.0 35.0 41.0 30.0 41.0 54-55 36.0226227582222 39.0 35.0 40.0 29.0 41.0 56-57 35.74322522292539 38.5 35.0 40.0 28.5 41.0 58-59 35.42897474221357 38.0 34.5 40.0 28.0 41.0 60-61 35.020240541197104 38.0 34.0 40.0 27.5 41.0 62-63 34.69509392580636 37.0 34.0 40.0 27.0 41.0 64-65 34.421946673583975 37.0 34.0 39.5 26.0 41.0 66-67 33.96832476218318 36.0 33.0 39.0 26.0 41.0 68-69 33.55008612324614 36.0 33.0 39.0 26.0 40.0 70-71 33.088997223468894 35.0 32.0 38.5 25.0 40.0 72-73 32.57012545858055 35.0 32.0 37.5 24.0 39.5 74-75 32.09825859482547 35.0 31.5 37.0 22.5 39.0 76-77 30.13528500275183 33.0 29.5 35.0 20.0 37.5 78-79 31.11594275103039 34.0 31.0 36.0 20.5 38.0 80-81 31.122468079499647 34.0 31.0 35.5 20.5 37.0 82-83 30.796088426270952 34.0 31.0 35.0 20.0 37.0 84-85 30.393171627502895 34.0 30.5 35.0 18.0 36.5 86-87 29.801164413737446 34.0 30.0 35.0 12.5 36.0 88-89 29.227901461281597 34.0 29.0 35.0 7.0 35.5 90-91 28.7656609118496 34.0 29.0 35.0 2.0 35.0 92-93 28.392396861408137 33.0 29.0 35.0 2.0 35.0 94-95 27.982878149674654 33.0 28.0 35.0 2.0 35.0 96-97 27.343990793390674 33.0 27.0 35.0 2.0 35.0 98-99 26.41087650818617 33.0 25.5 34.5 2.0 35.0 100-101 22.017957417373154 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11198879671403716 1101 2 0.1312411989034814 1101 3 0.13049771911787644 1101 4 0.06732846621965649 1101 5 0.1257469000453071 1101 6 0.12309242455935276 1101 7 0.03086444557729351 1101 8 0.11498792290213089 1101 9 0.1814208357364251 1101 10-11 0.2236605100983411 1101 12-13 0.23633929643937535 1101 14-15 0.2587268795428912 1101 16-17 0.21341270930886225 1101 18-19 0.22718415704802908 1101 20-21 0.23664065948417345 1101 22-23 0.21978472584289221 1101 24-25 0.21049777950911164 1101 26-27 0.2287158004531804 1101 28-29 0.26243772595030634 1101 30-31 0.22477963429111725 1101 32-33 0.25736846457184726 1101 34-35 0.3768476165932597 1101 36-37 0.3238399417323734 1101 38-39 0.39268407715359643 1101 40-41 0.41815396248721015 1101 42-43 0.3811916245037281 1101 44-45 0.5174040184444308 1101 46-47 0.4805412138888556 1101 48-49 0.28621439086360567 1101 50-51 0.4165096399324 1101 52-53 0.541724080083192 1101 54-55 0.5732736738074564 1101 56-57 0.28376168038077765 1101 58-59 0.35070102797737945 1101 60-61 0.6025549883224883 1101 62-63 0.41409710975087677 1101 64-65 0.23997242689367226 1101 66-67 0.38474860195576355 1101 68-69 0.3314374102295474 1101 70-71 0.3377992464096664 1101 72-73 0.21282917683438285 1101 74-75 0.07506877753750274 1101 76-77 -0.08264365554026654 1101 78-79 0.22803253843533255 1101 80-81 0.33282571666859795 1101 82-83 0.36464122865914916 1101 84-85 0.3432288670369239 1101 86-87 0.4390432030102467 1101 88-89 0.5644901666073459 1101 90-91 0.5686144863051901 1101 92-93 0.45741194146804176 1101 94-95 0.43374096030799336 1101 96-97 0.32396394896900205 1101 98-99 0.41790511226071914 1101 100-101 0.7979510560900493 1102 1 0.07236567643452929 1102 2 0.08470671354567116 1102 3 0.11125518025642833 1102 4 0.09513215795764296 1102 5 0.04263988070555769 1102 6 0.10476154344485877 1102 7 0.12981695694454487 1102 8 0.17303336200453145 1102 9 0.21821924465231035 1102 10-11 0.1467381554991647 1102 12-13 0.15809899814170336 1102 14-15 0.16101757259483662 1102 16-17 0.1532687917945097 1102 18-19 0.13277591283389256 1102 20-21 0.22177625327775985 1102 22-23 0.24030836988371362 1102 24-25 0.22598380916994643 1102 26-27 0.1820032834976857 1102 28-29 0.18415774232751403 1102 30-31 0.1148144876942041 1102 32-33 0.2086602516470677 1102 34-35 0.3275688527630862 1102 36-37 0.2870403006168871 1102 38-39 0.3390080603297463 1102 40-41 0.3879221152092569 1102 42-43 0.2843420104711285 1102 44-45 0.2931388895402094 1102 46-47 0.3855052277119242 1102 48-49 0.33011838500886626 1102 50-51 0.4464060739807394 1102 52-53 0.38812249661322795 1102 54-55 0.3065572715991536 1102 56-57 0.31991467164304055 1102 58-59 0.23178701052570005 1102 60-61 0.1294440905282599 1102 62-63 0.27814555338080993 1102 64-65 0.2388044716294857 1102 66-67 0.2933838907587045 1102 68-69 0.5259116918444349 1102 70-71 0.3054208224456403 1102 72-73 0.3139398645598206 1102 74-75 0.42525735506419693 1102 76-77 0.12307572213264706 1102 78-79 0.17523219063212636 1102 80-81 0.39896279810951185 1102 82-83 0.3502154855110682 1102 84-85 0.40300665131089275 1102 86-87 0.39352744929584205 1102 88-89 0.4260976653152362 1102 90-91 0.6184328685817739 1102 92-93 0.708059016217689 1102 94-95 0.7480483103191347 1102 96-97 0.5363070368476066 1102 98-99 0.5090447558270164 1102 100-101 0.9704724695946538 1103 1 0.08403871201591784 1103 2 0.1253076533662636 1103 3 0.11751224711452579 1103 4 0.0641749033576886 1103 5 0.13556903357161332 1103 6 0.016464650233466216 1103 7 0.11066700511984351 1103 8 0.09965082556371385 1103 9 0.19523790920210615 1103 10-11 0.16411315141475313 1103 12-13 0.08997166639989018 1103 14-15 0.23827523938430062 1103 16-17 0.14806023644482735 1103 18-19 0.23550101439678173 1103 20-21 0.1676900439532929 1103 22-23 0.18888316384285986 1103 24-25 0.18919401752376785 1103 26-27 0.2455722896202417 1103 28-29 0.2846676010558653 1103 30-31 0.34248802617631213 1103 32-33 0.299481392982905 1103 34-35 0.15960121522049064 1103 36-37 0.2193517630213151 1103 38-39 0.3294263669110933 1103 40-41 0.3296694347998468 1103 42-43 0.41444708719748746 1103 44-45 0.4026753351633232 1103 46-47 0.5269927456729633 1103 48-49 0.3402977706240833 1103 50-51 0.3673586478886506 1103 52-53 0.4901665591420965 1103 54-55 0.4512039249623925 1103 56-57 0.28051550199316466 1103 58-59 0.34804079658687925 1103 60-61 0.4105536915365846 1103 62-63 0.5307766181486784 1103 64-65 0.42857029272521174 1103 66-67 0.5095712398532442 1103 68-69 0.49550307505859337 1103 70-71 0.3922428912949414 1103 72-73 0.4299854908578098 1103 74-75 0.3170714184704835 1103 76-77 0.33546369942183674 1103 78-79 0.5511755234076041 1103 80-81 0.558753032779375 1103 82-83 0.6193416772671796 1103 84-85 0.625395212442605 1103 86-87 0.5308853595244187 1103 88-89 0.6707177306226662 1103 90-91 0.8696877982776776 1103 92-93 0.8578314586183922 1103 94-95 0.7318090290956256 1103 96-97 0.5364973661928438 1103 98-99 0.6951348984587469 1103 100-101 1.2330848779400831 1104 1 0.0011549085215634136 1104 2 0.005030893030408379 1104 3 0.015373380510538937 1104 4 -0.02226460251039697 1104 5 0.06030575913092662 1104 6 0.005390062161318099 1104 7 -0.05479839439298928 1104 8 0.07412926809710285 1104 9 0.0680521232993172 1104 10-11 0.04407416392778174 1104 12-13 0.11042648411342526 1104 14-15 0.17146016131857067 1104 16-17 0.06669563809943924 1104 18-19 0.0403008495893431 1104 20-21 0.09255246241172443 1104 22-23 0.03910351211656149 1104 24-25 0.03305348832490296 1104 26-27 0.042836903575143026 1104 28-29 -0.035187702367700524 1104 30-31 0.00492953587054501 1104 32-33 -0.011033612117408609 1104 34-35 -0.044342546963100915 1104 36-37 -0.09399655060651213 1104 38-39 0.04960084862073444 1104 40-41 0.07988088927995562 1104 42-43 0.023436443686151165 1104 44-45 -0.025190099924692788 1104 46-47 0.026284418541081322 1104 48-49 0.04962772310582153 1104 50-51 0.021036140782690893 1104 52-53 -0.02322132279188338 1104 54-55 0.06142207701359581 1104 56-57 0.016321279691887014 1104 58-59 0.07169202556395504 1104 60-61 0.19006792705149422 1104 62-63 0.17528397458652023 1104 64-65 0.08803011274386563 1104 66-67 0.025152621694658706 1104 68-69 0.07246116169503836 1104 70-71 -0.01733944526798581 1104 72-73 -0.002850939572752509 1104 74-75 0.09295508039016198 1104 76-77 -0.25184249755572097 1104 78-79 -0.05921823569038409 1104 80-81 0.036153721776116754 1104 82-83 0.22258636912445695 1104 84-85 0.10944628096838116 1104 86-87 -0.13827777080051717 1104 88-89 0.08564314981075327 1104 90-91 0.25388153051278195 1104 92-93 0.1688950655780559 1104 94-95 0.19553278786690242 1104 96-97 0.2828096182102833 1104 98-99 0.27238384844658725 1104 100-101 0.7727484747354154 1105 1 -0.08097033380937546 1105 2 -0.003906842173151404 1105 3 0.017242374072566236 1105 4 0.021360149975109266 1105 5 0.022060235125906047 1105 6 0.08094029593939922 1105 7 0.030155446098824257 1105 8 0.0859412814913938 1105 9 -0.1627587004703699 1105 10-11 -0.009525033658761117 1105 12-13 0.0036678807171597327 1105 14-15 0.04485536679447222 1105 16-17 -0.030481498041304178 1105 18-19 -0.021075940031117568 1105 20-21 -0.03344925852670144 1105 22-23 -0.10792236157070789 1105 24-25 -0.05014896038387917 1105 26-27 -0.054526678361220604 1105 28-29 -0.03035064076640026 1105 30-31 -0.0701868996098085 1105 32-33 -0.13098645477595028 1105 34-35 -0.04304892739416033 1105 36-37 -0.020259263261628746 1105 38-39 0.02896254633686368 1105 40-41 0.06282166593117466 1105 42-43 0.053355891327115046 1105 44-45 -0.0748554694080994 1105 46-47 0.09410063449812611 1105 48-49 -0.0277025739634027 1105 50-51 0.11280570090668363 1105 52-53 0.05478720933056991 1105 54-55 0.14512311838868186 1105 56-57 0.07685412266143743 1105 58-59 0.10095186653265387 1105 60-61 -0.015427736909352063 1105 62-63 -0.07777917918866706 1105 64-65 -0.10361008542322736 1105 66-67 0.03745674831841228 1105 68-69 0.017055335126237026 1105 70-71 -0.045179479634924746 1105 72-73 -0.060786338291386244 1105 74-75 0.043738735881099444 1105 76-77 -0.08701372315416478 1105 78-79 0.1457922804356393 1105 80-81 0.17222529752332605 1105 82-83 0.20511651146472687 1105 84-85 0.08645432324540891 1105 86-87 0.08604551681391115 1105 88-89 0.1605122327890136 1105 90-91 0.2548042023215267 1105 92-93 0.31102783053518834 1105 94-95 0.1608183949642168 1105 96-97 0.08346731344867564 1105 98-99 0.2839676236775155 1105 100-101 0.8305179998704446 1106 1 -0.06974998418773026 1106 2 -0.11316297938844144 1106 3 -0.12586630664204534 1106 4 -0.026584223962593967 1106 5 0.003560038624875972 1106 6 0.002533255945806445 1106 7 -0.03147688295153728 1106 8 0.02270643647688786 1106 9 0.016399158068914232 1106 10-11 0.05787450675411776 1106 12-13 0.05409538779495193 1106 14-15 -0.011922753191306867 1106 16-17 -0.02421704989630058 1106 18-19 -0.058311242505027394 1106 20-21 -0.02158065889067018 1106 22-23 0.05966837622182197 1106 24-25 0.0985219041455565 1106 26-27 0.09149713268239168 1106 28-29 0.16501335079531998 1106 30-31 0.09086689348586674 1106 32-33 0.09768841174102505 1106 34-35 0.08113886313323349 1106 36-37 -0.02680856749778826 1106 38-39 0.14376702155394838 1106 40-41 0.1906075295594647 1106 42-43 0.23344063708982787 1106 44-45 0.1323514640118404 1106 46-47 0.14672954762214374 1106 48-49 0.04018505091487867 1106 50-51 0.06402963948821849 1106 52-53 0.22821407465102084 1106 54-55 0.17426464356208982 1106 56-57 0.11429460913290512 1106 58-59 0.1829290924387479 1106 60-61 0.26291808425217766 1106 62-63 0.2055234430362276 1106 64-65 0.057466666690089596 1106 66-67 0.21264484799073813 1106 68-69 0.2084105335797588 1106 70-71 0.0878608309243134 1106 72-73 0.19653469401489332 1106 74-75 0.19075299190566852 1106 76-77 -0.006642689784033706 1106 78-79 0.1367198701189558 1106 80-81 0.292875717497342 1106 82-83 0.3803384825327676 1106 84-85 0.399299842771601 1106 86-87 0.3595558637821661 1106 88-89 0.4483846382244572 1106 90-91 0.5947697583193126 1106 92-93 0.5242212398030297 1106 94-95 0.6230105250979676 1106 96-97 0.4828255013462126 1106 98-99 0.418544350133061 1106 100-101 1.0107121432010508 1107 1 -0.0023938720092289145 1107 2 0.006134213920539366 1107 3 -0.03727854722466617 1107 4 -0.042348295312280015 1107 5 -0.13123497737983314 1107 6 -0.04919171577155623 1107 7 -0.08146980697380002 1107 8 -0.06763240497702583 1107 9 -0.019511835184026438 1107 10-11 0.014789694603337011 1107 12-13 0.03534017801445799 1107 14-15 0.08061892640671431 1107 16-17 0.03611693780054992 1107 18-19 0.04402840303374944 1107 20-21 0.02042886771985053 1107 22-23 0.057163542002037104 1107 24-25 -0.0021827611018920834 1107 26-27 -0.05409979471065185 1107 28-29 0.03991358492545771 1107 30-31 0.03516022382941486 1107 32-33 -0.04867646621595867 1107 34-35 -0.08072217394899184 1107 36-37 0.05734747881879798 1107 38-39 0.003334474656163877 1107 40-41 0.012223252668285056 1107 42-43 2.0217369971220478E-4 1107 44-45 -0.11038594819515168 1107 46-47 -0.2096578937669804 1107 48-49 -0.15327492733180748 1107 50-51 -0.061846394798052984 1107 52-53 -0.0037760531800543617 1107 54-55 -0.12359931645217159 1107 56-57 -0.04439353477042829 1107 58-59 -0.0684882502758839 1107 60-61 -0.030169687506564458 1107 62-63 -0.13910483755380199 1107 64-65 -0.12085536171456113 1107 66-67 -0.03526893789412355 1107 68-69 -0.007703328612691962 1107 70-71 -0.0920954040179538 1107 72-73 -0.04156963568652117 1107 74-75 -0.10091919742814781 1107 76-77 -0.33307533928157085 1107 78-79 -0.0061828855197916255 1107 80-81 0.2056161837102657 1107 82-83 0.21593748281201286 1107 84-85 0.09760104327370911 1107 86-87 0.06897675632409772 1107 88-89 0.1680165651288732 1107 90-91 0.4255200309320948 1107 92-93 0.41720367273076775 1107 94-95 0.5584718049531574 1107 96-97 0.5522978686279139 1107 98-99 0.4575002963878205 1107 100-101 1.1229851615810276 1108 1 0.0641719239695604 1108 2 0.009348567454026124 1108 3 0.0026218443551186965 1108 4 0.017668656720992715 1108 5 0.011660919309100848 1108 6 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-3.0839025465099503 2316 100-101 -3.7345921495844685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 32.0 5 71.0 6 169.0 7 411.0 8 986.0 9 1778.0 10 2960.0 11 4529.0 12 6438.0 13 9100.0 14 11027.0 15 14543.0 16 19092.0 17 26610.0 18 25883.0 19 19354.0 20 18494.0 21 19559.0 22 21655.0 23 24049.0 24 28107.0 25 33546.0 26 40510.0 27 50076.0 28 61310.0 29 73728.0 30 90175.0 31 109159.0 32 135922.0 33 174219.0 34 234239.0 35 334985.0 36 531353.0 37 835812.0 38 635995.0 39 47139.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.351232703944895 19.509939133761304 14.648467429782233 23.49036073251157 2 15.655929739900934 20.606444901023462 39.70450462320565 24.033120735869954 3 19.34436534373126 24.149836606016688 29.855546268427286 26.650251781824768 4 13.436351833018348 16.84905580100109 36.21049482093913 33.504097545041425 5 14.114620161711807 37.10722191234535 34.13315421474028 14.64500371120256 6 32.67747907994296 35.778657081252305 17.045128580977124 14.498735257827608 7 28.895714699419578 32.4247502425862 20.54553414941114 18.13400090858309 8 25.90658612942231 36.07387796164462 19.405248645703377 18.6142872632297 9 26.150256585511805 16.952376246728345 20.26115587689934 36.6362112908605 10-11 25.15209159395943 26.585333382742686 26.952595843849974 21.309979179447904 12-13 25.852963257284106 23.74378780330137 27.73965937741471 22.663589561999817 14-15 22.74554509815655 25.181324692425694 25.82674383605846 26.246386373359293 16-17 22.46926515824882 28.48411472730497 26.529591258035417 22.517028856410796 18-19 22.4312009080341 27.164054147160265 28.444977882452406 21.95976706235323 20-21 23.049847174128164 26.41920816022904 28.437113560534005 22.09383110510879 22-23 22.827175692794608 26.32513824325048 28.33025211122045 22.517433952734464 24-25 22.68125340547099 26.41329276276511 28.41169519370525 22.493758638058654 26-27 22.6013200531974 26.56265997993431 28.37881052474105 22.457209442127237 28-29 22.460613197512515 26.72636963411002 28.456424382417406 22.356592785960057 30-31 22.601704348160258 26.51168599807578 28.369244325130012 22.517365328633954 32-33 22.43644378931303 26.630666463539328 28.27292353765473 22.65996620949291 34-35 22.785641454147477 26.645526264392373 28.18676535237763 22.382066929082526 36-37 22.80892833906803 26.475744343952268 28.204375390159818 22.51095192681988 38-39 22.6512779290461 26.616868915979918 28.346777270987722 22.385075883986257 40-41 22.76044283035908 26.546872984108944 28.227566821212598 22.465117364319383 42-43 22.614604998664568 26.641148412042448 27.97814611147421 22.766100477818775 44-45 22.659075715034895 26.68418234698862 27.990652813879908 22.66608912409658 46-47 22.77437523023468 26.86316470227191 27.619020692161616 22.743439375331796 48-49 22.875999632722856 26.832395216558325 27.550822798248326 22.740782352470497 50-51 22.769809326759106 27.058608775017927 27.506315171852965 22.665266726370003 52-53 22.931964694272768 27.083640426183113 27.27339978891227 22.71099509063185 54-55 22.725063031236317 26.873472255962405 27.389319619493087 23.012145093308188 56-57 22.71102254027205 27.25439091307111 27.27254885006595 22.76203769659089 58-59 22.831149937208775 26.946726964541828 27.379924650530807 22.842198447718587 60-61 22.820583029836374 26.84815407282966 27.582668179176096 22.748594718157868 62-63 22.77809573611014 26.853049723650745 27.65341260789424 22.71544193234487 64-65 22.615716566587388 26.849309578555314 27.528634486389553 23.006339368467746 66-67 22.53176628054904 27.027831240652382 27.404172057751158 23.036230421047417 68-69 22.456488870637635 26.962873211352477 27.564647694632082 23.01599022337781 70-71 22.550266817401813 26.876522854779445 27.396419111595016 23.176791216223727 72-73 22.572964574866834 26.82277277050591 27.278628945371096 23.325633709256156 74-75 22.522210190129933 27.046789284209456 27.111460636529706 23.3195398891309 76-77 22.599792166359258 26.819032196624587 27.319405016425787 23.261770620590372 78-79 22.51091020711174 26.895868511777344 27.309328887587775 23.283892393523146 80-81 22.475971271206564 26.99458006854175 27.268458853675575 23.26098980657611 82-83 22.49488407330701 26.930649856507006 27.246005047988834 23.328461022197153 84-85 22.529731391545784 26.768120536860064 27.144317855853707 23.557830215740445 86-87 22.404714912747917 26.66306910547594 27.452534940488356 23.479681041287787 88-89 22.467302928051346 26.588375388427 27.335983204706633 23.60833847881502 90-91 22.34572459527801 26.700971283196388 27.361507757857183 23.59179636366841 92-93 22.345695278799134 26.697300465134155 27.26328459649715 23.693719659569563 94-95 22.275890843760767 26.548084220986073 27.27298804430921 23.90303689094395 96-97 22.210087884659224 26.459138842060288 27.31642361468054 24.014349658599947 98-99 22.08197286054073 26.460025775212152 27.21283215780249 24.24516920644463 100-101 22.270396479628396 25.953764516269377 27.781270754561216 23.994568249541008 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 651.0 1 450.0 2 303.0 3 383.0 4 364.5 5 361.0 6 625.0 7 1277.5 8 2103.5 9 2620.5 10 2566.0 11 2461.5 12 2719.0 13 2972.0 14 3174.0 15 3462.5 16 3669.5 17 3877.0 18 4162.5 19 4628.0 20 5332.5 21 6288.0 22 7724.0 23 9499.0 24 11541.5 25 14548.0 26 18427.0 27 22803.0 28 27460.0 29 32952.0 30 39120.0 31 45286.0 32 50347.5 33 55450.5 34 62758.5 35 70285.0 36 78076.0 37 86271.0 38 92695.0 39 96471.5 40 100042.5 41 105954.0 42 112035.5 43 117890.5 44 124863.0 45 130310.0 46 133505.5 47 139158.0 48 152173.5 49 154768.0 50 146465.0 51 142614.0 52 138698.0 53 136135.0 54 132730.5 55 124728.5 56 113593.5 57 101544.0 58 91338.0 59 80413.5 60 67746.0 61 54678.0 62 42899.5 63 33834.5 64 25429.5 65 18021.5 66 13028.0 67 10111.5 68 7474.5 69 5051.0 70 3380.5 71 2283.5 72 1516.5 73 1027.5 74 723.0 75 464.0 76 281.0 77 117.5 78 55.5 79 34.0 80 21.0 81 15.5 82 11.0 83 8.0 84 5.0 85 2.5 86 3.5 87 3.0 88 3.0 89 2.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40490964264685897 2 0.0 3 0.0 4 0.0 5 3.019460422422513E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001468555750905495 16-17 0.0027724136605879438 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024567427982437723 36-37 0.008152543140540786 38-39 0.013752269742124356 40-41 0.002882212221403308 42-43 6.038920844845026E-4 44-45 3.705701427518539E-4 46-47 3.5684532264993336E-4 48-49 2.6077158193648975E-4 50-51 6.176169045864232E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.7449640203841026E-4 60-61 0.0022645953168168847 62-63 0.0 64-65 1.7842266132496668E-4 66-67 0.0016607032323323822 68-69 0.0016195287720266209 70-71 1.3724820101920514E-5 72-73 0.0 74-75 0.0 76-77 0.124840963647069 78-79 4.117446030576154E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14783003731778585 88-89 0.44101964433501184 90-91 0.3410755043528267 92-93 0.0 94-95 0.0 96-97 5.901672643825822E-4 98-99 0.0 100-101 2.882212221403308E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3643035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.37585873578009 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18328768360858 35.14735957014253 2 14.864368236658008 14.678818924996847 3 5.6181683057061536 8.322056538491521 4 2.6352908955157313 5.204790039386886 5 1.4889819897494907 3.6759882192995805 6 0.92014197659832 2.725968015202807 7 0.6370515485520503 2.201847708810119 8 0.42259455522864997 1.6692775249183711 9 0.3328753983937687 1.4792407782936516 >10 1.7378329978648428 15.316507098211964 >50 0.10673023670051435 3.6104118016515017 >100 0.049836637158856764 4.303147329861989 >500 0.002338443284374593 0.7539985621855786 >1k 3.34063320450816E-4 0.2899211989437259 >5k 1.6703166022540795E-4 0.6206666896029298 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9281 0.25476011073184857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8250 0.22645953168168848 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5046 0.13851088446858184 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.489928040768206E-5 2 0.0 0.0 0.0 0.0 5.489928040768206E-5 3 0.0 0.0 0.0 0.0 5.489928040768206E-5 4 0.0 0.0 0.0 2.744964020384103E-5 5.489928040768206E-5 5 0.0 0.0 0.0 2.744964020384103E-5 5.489928040768206E-5 6 5.489928040768206E-5 0.0 0.0 2.744964020384103E-5 5.489928040768206E-5 7 5.489928040768206E-5 0.0 0.0 2.744964020384103E-5 5.489928040768206E-5 8 5.489928040768206E-5 0.0 0.0 2.744964020384103E-5 5.489928040768206E-5 9 5.489928040768206E-5 0.0 0.0 8.234892061152308E-5 5.489928040768206E-5 10-11 5.489928040768206E-5 0.0 0.0 9.60737407134436E-5 5.489928040768206E-5 12-13 6.862410050960257E-5 0.0 0.0 1.2352338091728462E-4 1.2352338091728462E-4 14-15 1.0979856081536411E-4 0.0 0.0 1.6469784122304616E-4 1.5097302112112565E-4 16-17 1.2352338091728462E-4 0.0 0.0 1.7842266132496668E-4 1.6469784122304616E-4 18-19 1.5097302112112565E-4 0.0 0.0 2.058723015288077E-4 1.6469784122304616E-4 20-21 1.6469784122304616E-4 0.0 0.0 2.3332194173264872E-4 2.3332194173264872E-4 22-23 1.6469784122304616E-4 0.0 0.0 3.156708623441718E-4 3.156708623441718E-4 24-25 1.6469784122304616E-4 0.0 0.0 3.980197829556949E-4 3.293956824460923E-4 26-27 1.6469784122304616E-4 0.0 0.0 6.450665447902641E-4 3.293956824460923E-4 28-29 2.1959712163072823E-4 0.0 0.0 0.0028135881208937053 3.293956824460923E-4 30-31 2.1959712163072823E-4 0.0 0.0 0.00853683810339456 3.4312050254801284E-4 32-33 2.1959712163072823E-4 0.0 0.0 0.019351996343707926 3.5684532264993336E-4 34-35 2.1959712163072823E-4 0.0 0.0 0.03576688118560486 3.842949628537744E-4 36-37 2.7449640203841026E-4 0.0 0.0 0.06394393685484767 4.117446030576154E-4 38-39 2.7449640203841026E-4 0.0 0.0 0.10547524248325915 4.117446030576154E-4 40-41 2.7449640203841026E-4 0.0 0.0 0.153155267517331 4.117446030576154E-4 42-43 3.156708623441718E-4 0.0 0.0 0.20131566125497008 4.117446030576154E-4 44-45 3.5684532264993336E-4 0.0 0.0 0.2601127905715976 4.6664388346529743E-4 46-47 4.940935236691385E-4 0.0 0.0 0.3341032957410511 5.215431638729795E-4 48-49 5.489928040768205E-4 0.0 0.0 0.41557382786605124 5.215431638729795E-4 50-51 5.489928040768205E-4 0.0 0.0 0.5040302934229289 5.489928040768205E-4 52-53 5.489928040768205E-4 0.0 0.0 0.5930494766039854 5.901672643825821E-4 54-55 5.489928040768205E-4 0.0 0.0 0.6951072388818664 6.038920844845026E-4 56-57 5.489928040768205E-4 0.0 0.0 0.8180953518151761 6.038920844845026E-4 58-59 5.489928040768205E-4 0.0 0.0 0.954863184130814 6.038920844845026E-4 60-61 5.489928040768205E-4 0.0 0.0 1.1115457304143386 6.038920844845026E-4 62-63 5.489928040768205E-4 0.0 0.0 1.2725378702098662 6.038920844845026E-4 64-65 5.489928040768205E-4 0.0 0.0 1.442519767172152 6.038920844845026E-4 66-67 5.764424442806616E-4 0.0 0.0 1.63689066945555 6.038920844845026E-4 68-69 5.764424442806616E-4 0.0 0.0 1.864489361205698 6.038920844845026E-4 70-71 5.901672643825821E-4 0.0 0.0 2.1194141697787696 6.587913648921847E-4 72-73 6.038920844845026E-4 0.0 0.0 2.3806798452389284 7.411402855037077E-4 74-75 6.038920844845026E-4 0.0 0.0 2.644635585439064 7.411402855037077E-4 76-77 6.038920844845026E-4 0.0 0.0 2.9399525395720874 7.411402855037077E-4 78-79 6.038920844845026E-4 0.0 0.0 3.2756341896248595 7.411402855037077E-4 80-81 6.862410050960257E-4 0.0 0.0 3.6482767802121034 7.548651056056283E-4 82-83 6.862410050960257E-4 0.0 0.0 4.036798987657269 7.685899257075488E-4 84-85 6.862410050960257E-4 0.0 0.0 4.430207231058719 7.685899257075488E-4 86-87 6.862410050960257E-4 0.0 0.0 4.843639986988871 7.685899257075488E-4 88-89 6.862410050960257E-4 0.0 0.0 5.292825899284525 7.685899257075488E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10575 0.0 30.637564 1 GTATCAA 15450 0.0 29.32768 1 ATCAACG 18460 0.0 24.060678 3 TCAACGC 18640 0.0 23.777416 4 TATCAAC 18890 0.0 23.63858 2 CAACGCA 18725 0.0 23.593456 5 GTACATG 12290 0.0 23.222054 1 AACGCAG 19175 0.0 23.188248 6 TACATGG 12340 0.0 22.149864 2 CGCGAAA 55 0.0028557417 21.620886 72-73 ACGCAGA 21430 0.0 20.416086 7 CGCAGAG 21585 0.0 20.137575 8 ACATGGG 12965 0.0 19.654657 3 GAGTACT 10175 0.0 19.588287 12-13 AGTACTT 10530 0.0 19.536303 12-13 GTACTTT 10725 0.0 18.539513 14-15 CATGGGG 7055 0.0 18.49692 4 AGAGTAC 18595 0.0 18.20872 10-11 GCAGAGT 23185 0.0 18.175049 9 GGACCGT 395 3.6561687E-10 16.818787 6 >>END_MODULE