FastQCFastQC Report
Fri 27 May 2016
SRR939013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491983
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124720.27765020482045455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91580.2038743245466423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79790.17762756448543995No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59630.13274760835025423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50350.11208858092294649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48560.10810370386530849No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45960.10231561428438177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG111150.024.2911742
GTACATG118450.023.7620771
ACATGGG115400.022.5756343
GAGTACT101200.019.58072512-13
CATGGGG81900.018.681074
AGAGTAC143750.018.64276110-11
GTACTTT106100.018.61000614-15
TACCGTA2854.4412445E-818.2743637
AGTACTT105800.017.90144512-13
AGTACAT83850.017.5122412
GAGTACA92900.017.2178521
GTGTAGC25450.017.1579281
GTACCGT4559.094947E-1216.649576
GTATCAA252300.016.4421651
TAGCCCT29750.016.2403854
GTATAGG11400.015.821351
CAACGCA269400.015.1035385
GTATAGC15750.015.0679531
TCAACGC272850.014.8952044
GGTATCA216700.014.894121
Produced by FastQC (version 0.11.5)