##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4491983 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71068367801036 33.0 31.0 34.0 30.0 34.0 2 31.79183069036548 33.0 31.0 34.0 30.0 34.0 3 31.855062007135825 34.0 31.0 34.0 30.0 34.0 4 35.38276636398668 37.0 35.0 37.0 33.0 37.0 5 35.41097350546518 37.0 35.0 37.0 33.0 37.0 6 35.48580326328038 37.0 35.0 37.0 33.0 37.0 7 35.39798681339622 37.0 35.0 37.0 33.0 37.0 8 35.380109853487866 37.0 35.0 37.0 33.0 37.0 9 36.981900866499274 39.0 37.0 39.0 33.0 39.0 10-11 36.85921574057605 39.0 37.0 39.0 33.0 39.0 12-13 36.73893567718311 39.0 37.0 39.0 32.0 39.0 14-15 37.950231556976064 40.0 38.0 41.0 33.0 41.0 16-17 37.842866502388816 40.0 38.0 41.0 32.5 41.0 18-19 37.723287910929315 40.0 38.0 41.0 32.0 41.0 20-21 37.83449258378761 40.0 38.0 41.0 32.0 41.0 22-23 37.7681159968771 40.0 38.0 41.0 32.0 41.0 24-25 37.68430434843587 40.0 38.0 41.0 32.0 41.0 26-27 37.558340603693296 40.0 37.0 41.0 32.0 41.0 28-29 37.42801631261739 40.0 37.0 41.0 31.5 41.0 30-31 37.2512340318296 40.0 37.0 41.0 31.0 41.0 32-33 37.05811097237011 40.0 36.5 41.0 30.5 41.0 34-35 36.73438356734654 40.0 36.0 41.0 30.0 41.0 36-37 36.47237222402667 39.0 36.0 41.0 30.0 41.0 38-39 36.231305194164804 39.0 35.5 41.0 29.5 41.0 40-41 36.08995548291256 39.0 35.0 41.0 29.0 41.0 42-43 35.88596806800025 39.0 35.0 40.5 28.0 41.0 44-45 35.86349302746693 39.0 35.0 41.0 28.0 41.0 46-47 35.701067657646966 39.0 35.0 41.0 27.0 41.0 48-49 35.487537686585185 39.0 35.0 41.0 26.0 41.0 50-51 34.390333066710184 38.0 34.0 40.0 25.0 40.5 52-53 34.49892475105093 38.0 33.5 39.5 25.0 40.5 54-55 34.98562639261992 39.0 34.5 40.0 26.0 41.0 56-57 34.85927072742706 38.0 34.0 40.0 25.5 41.0 58-59 34.62847043276878 38.0 34.0 40.0 25.0 41.0 60-61 34.28851901264987 38.0 34.0 40.0 23.5 41.0 62-63 33.89928824307661 37.0 33.5 40.0 23.0 41.0 64-65 33.49237530507128 37.0 33.0 39.5 22.0 41.0 66-67 33.060827256024794 36.0 32.5 39.0 21.5 41.0 68-69 32.59847577339451 35.5 32.0 39.0 19.5 40.5 70-71 32.14366249827749 35.0 31.5 38.5 18.0 40.0 72-73 31.699170722596236 35.0 31.0 37.5 17.0 40.0 74-75 31.223533682117676 35.0 31.0 37.0 12.5 39.0 76-77 30.781103356802554 35.0 30.5 36.5 8.0 39.0 78-79 30.370426268309565 34.5 30.0 36.0 7.0 38.5 80-81 29.908914726525012 34.0 30.0 35.5 2.0 37.0 82-83 29.433514663791023 34.0 29.0 35.0 2.0 37.0 84-85 29.003579488168143 34.0 29.0 35.0 2.0 36.5 86-87 28.54926777327519 34.0 29.0 35.0 2.0 36.0 88-89 28.06155822495321 34.0 27.5 35.0 2.0 36.0 90-91 27.583852053758886 33.5 26.5 35.0 2.0 35.0 92-93 27.055887232876884 33.0 25.5 35.0 2.0 35.0 94-95 26.486955204416404 33.0 25.0 35.0 2.0 35.0 96-97 25.799361662766756 32.5 23.5 35.0 2.0 35.0 98-99 24.877726273674675 32.0 10.5 34.5 2.0 35.0 100-101 21.59162590330373 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16695750176884872 1101 2 0.28129656054093743 1101 3 0.2723014101119503 1101 4 0.24681900195082562 1101 5 0.12139186046432826 1101 6 0.2337222162668624 1101 7 0.25669615569826476 1101 8 0.18030585466571125 1101 9 0.30082948701936374 1101 10-11 0.20997091572518656 1101 12-13 0.24664378688081 1101 14-15 0.29516218088981105 1101 16-17 0.34562747091319324 1101 18-19 0.47445874007783573 1101 20-21 0.3453282450293642 1101 22-23 0.41559005215994205 1101 24-25 0.5034941949962644 1101 26-27 0.5596537253567178 1101 28-29 0.47642110671690574 1101 30-31 0.35738568362106093 1101 32-33 0.25500946551387216 1101 34-35 0.23576127530478885 1101 36-37 0.24040300756905708 1101 38-39 0.4369208089532677 1101 40-41 0.1905186529330649 1101 42-43 0.22306610023706952 1101 44-45 0.35181655764407793 1101 46-47 0.26160384056179 1101 48-49 0.34596516171853153 1101 50-51 0.28521798592876024 1101 52-53 0.32089500047931807 1101 54-55 0.4174969302193645 1101 56-57 0.5176021692992592 1101 58-59 0.4595857895472335 1101 60-61 0.5193986948888138 1101 62-63 0.39047469977693794 1101 64-65 0.18876937286466244 1101 66-67 0.3091328037626866 1101 68-69 0.28060742483280876 1101 70-71 0.23397577169388484 1101 72-73 0.19160425986169116 1101 74-75 0.11555883067781636 1101 76-77 0.09906152455386774 1101 78-79 0.006223067732157972 1101 80-81 0.07888722372068102 1101 82-83 0.1061819096749268 1101 84-85 0.02036282766202291 1101 86-87 0.02877267225573732 1101 88-89 -0.11029475859486482 1101 90-91 -0.1269843509428057 1101 92-93 -0.06100173733041103 1101 94-95 -0.29218327302170977 1101 96-97 -0.4016677065062524 1101 98-99 -0.6402658269297916 1101 100-101 -0.07811129054308097 1102 1 0.22131817300496337 1102 2 0.2524617697113456 1102 3 0.3507698572875526 1102 4 0.11777149545117993 1102 5 0.27171093396940904 1102 6 0.25570283550580797 1102 7 0.3254742223491718 1102 8 0.2762642569346738 1102 9 0.3412454643296883 1102 10-11 0.42658637306386993 1102 12-13 0.32924837208525304 1102 14-15 0.4587168960871608 1102 16-17 0.5325809098165308 1102 18-19 0.5471365940130752 1102 20-21 0.4986017028407588 1102 22-23 0.5231296409096444 1102 24-25 0.5091666128643908 1102 26-27 0.5874249378360403 1102 28-29 0.4993471289338771 1102 30-31 0.5262582905697712 1102 32-33 0.4875785981066443 1102 34-35 0.4515495050377112 1102 36-37 0.5071248227427745 1102 38-39 0.5308702298939423 1102 40-41 0.5279093407137267 1102 42-43 0.4220734271101563 1102 44-45 0.5075716982727769 1102 46-47 0.6390559795360389 1102 48-49 0.5488722758759437 1102 50-51 0.5111692976753872 1102 52-53 0.4726321784514269 1102 54-55 0.48651134761477266 1102 56-57 0.4959760761770653 1102 58-59 0.35452788361482845 1102 60-61 0.4111500539055939 1102 62-63 0.3771208827124184 1102 64-65 0.33093434568432656 1102 66-67 0.38334693753721183 1102 68-69 0.3989009724574828 1102 70-71 0.25737449539986557 1102 72-73 0.11963545815996568 1102 74-75 0.10905918520393598 1102 76-77 0.1783571993352453 1102 78-79 0.1792318127097019 1102 80-81 0.22672461440846448 1102 82-83 0.20202213657164236 1102 84-85 0.08618651000662325 1102 86-87 0.08809670912645018 1102 88-89 -0.11171286306189288 1102 90-91 -0.09696780639069047 1102 92-93 -0.04256637925903206 1102 94-95 -0.13784623686004593 1102 96-97 -0.21849587951499672 1102 98-99 -0.40580588838098564 1102 100-101 -0.19628666279550444 1103 1 0.3079124931941344 1103 2 0.34290815897765725 1103 3 0.3162932845749751 1103 4 0.3484996685719395 1103 5 0.2150154310183865 1103 6 0.3619470877581463 1103 7 0.35562131988361756 1103 8 0.2171251561204457 1103 9 0.4703495381876195 1103 10-11 0.4211150493540785 1103 12-13 0.4508178138382348 1103 14-15 0.5609365488195905 1103 16-17 0.6152731744263136 1103 18-19 0.7052850099590202 1103 20-21 0.628952526875949 1103 22-23 0.5493429827411518 1103 24-25 0.5772963671828251 1103 26-27 0.6619569969662891 1103 28-29 0.6423795985975289 1103 30-31 0.6191596191745958 1103 32-33 0.631635244995266 1103 34-35 0.5469356808714636 1103 36-37 0.5296825690544154 1103 38-39 0.7531132432651262 1103 40-41 0.6549950520230539 1103 42-43 0.6256066467113541 1103 44-45 0.8577053596594482 1103 46-47 0.8653962338763179 1103 48-49 0.8733616014474208 1103 50-51 0.7786970296827675 1103 52-53 0.753598242517036 1103 54-55 0.8667865338110019 1103 56-57 0.6791314930336867 1103 58-59 0.6867123581712846 1103 60-61 0.8125942857712758 1103 62-63 0.7340709107720684 1103 64-65 0.6405250052373219 1103 66-67 0.7214434571324801 1103 68-69 0.6292986465869319 1103 70-71 0.6944005477487565 1103 72-73 0.7000622560778318 1103 74-75 0.6406979247711782 1103 76-77 0.7108133372296628 1103 78-79 0.659659478740501 1103 80-81 0.5286856815040686 1103 82-83 0.7384480148498866 1103 84-85 0.6675359719446305 1103 86-87 0.8307228459343285 1103 88-89 0.6907056715410853 1103 90-91 0.8149101066882416 1103 92-93 0.8327731649088754 1103 94-95 0.7230803924758966 1103 96-97 0.6690107153428606 1103 98-99 0.7347296084764459 1103 100-101 0.7427434422405597 1104 1 0.3038585869708932 1104 2 0.33415790068437445 1104 3 0.3243701018270926 1104 4 0.3593402516130908 1104 5 0.31581511808295204 1104 6 0.31654890724787776 1104 7 0.2850578592950299 1104 8 0.3202856714473157 1104 9 0.41162607609623336 1104 10-11 0.43743147769109214 1104 12-13 0.3919916145562681 1104 14-15 0.5386511421968905 1104 16-17 0.4799990686183335 1104 18-19 0.5371447271458365 1104 20-21 0.5089735273632527 1104 22-23 0.47776035485696866 1104 24-25 0.5066668000878991 1104 26-27 0.519418689447555 1104 28-29 0.5025238118886648 1104 30-31 0.5466163060148403 1104 32-33 0.5909020069864326 1104 34-35 0.737667896757138 1104 36-37 0.6699783383485141 1104 38-39 0.7415847437087777 1104 40-41 0.6955475123563843 1104 42-43 0.6991502869505268 1104 44-45 0.6939636319508438 1104 46-47 0.7964556562496483 1104 48-49 0.760353923921194 1104 50-51 0.6306167703618328 1104 52-53 0.7343640116674877 1104 54-55 0.8684470695803341 1104 56-57 0.8705893611168136 1104 58-59 0.8502893030506655 1104 60-61 0.9649665783880153 1104 62-63 0.9678055333434159 1104 64-65 0.9334182933688737 1104 66-67 0.8813014483123922 1104 68-69 0.8853857491605837 1104 70-71 0.9026192743711405 1104 72-73 0.8841331571705702 1104 74-75 0.824920100092374 1104 76-77 0.8594448800610444 1104 78-79 0.8520528657693731 1104 80-81 0.8240986413628058 1104 82-83 1.0231798981311577 1104 84-85 0.909612307417607 1104 86-87 0.8323436570249747 1104 88-89 0.8021479536271912 1104 90-91 0.7600715966233693 1104 92-93 0.8537305572903584 1104 94-95 0.8242258334198311 1104 96-97 0.6723323670054988 1104 98-99 0.7452047686735348 1104 100-101 0.9108900787923488 1105 1 0.24671959211517702 1105 2 0.2675448171535635 1105 3 0.2947542935582348 1105 4 0.3217581487399386 1105 5 0.26720578486838775 1105 6 0.2668957705207262 1105 7 0.2619907075402992 1105 8 0.30266513796978955 1105 9 0.33632189132737267 1105 10-11 0.3409391546453904 1105 12-13 0.385989960043041 1105 14-15 0.49533860764699256 1105 16-17 0.45933420708433204 1105 18-19 0.476454411645193 1105 20-21 0.5654382935217157 1105 22-23 0.3800658082800794 1105 24-25 0.443902897032757 1105 26-27 0.4063141833524284 1105 28-29 0.44786863586774217 1105 30-31 0.544653391397091 1105 32-33 0.4055318476670067 1105 34-35 0.5167929965919384 1105 36-37 0.5360014286323889 1105 38-39 0.660853136553321 1105 40-41 0.6786418640410616 1105 42-43 0.535612083138048 1105 44-45 0.57323150931024 1105 46-47 0.5939273903007276 1105 48-49 0.45748844117365906 1105 50-51 0.47347411426743236 1105 52-53 0.5187712436437835 1105 54-55 0.657947737014986 1105 56-57 0.5664353542118974 1105 58-59 0.5887571560808809 1105 60-61 0.6161832042292659 1105 62-63 0.6117077969271563 1105 64-65 0.687644247118854 1105 66-67 0.7140516705209947 1105 68-69 0.7342728674023107 1105 70-71 0.6361537295928485 1105 72-73 0.594084598037842 1105 74-75 0.5437379395021402 1105 76-77 0.634253762323155 1105 78-79 0.7227911893296977 1105 80-81 0.7578271206665086 1105 82-83 0.7096497501222139 1105 84-85 0.7167493341553275 1105 86-87 0.7873534232039283 1105 88-89 0.84943047874523 1105 90-91 0.939444064626084 1105 92-93 0.9783263651666694 1105 94-95 0.8642926717854742 1105 96-97 0.8829603012537497 1105 98-99 1.0603121610541457 1105 100-101 1.267567196794662 1106 1 0.17426944856340754 1106 2 0.1487295111850777 1106 3 0.2678526791629636 1106 4 0.1874698910688224 1106 5 0.18166113923804517 1106 6 0.2736909956328475 1106 7 0.22329445854743568 1106 8 0.19117809629627658 1106 9 0.26028883764435307 1106 10-11 0.2165623471140421 1106 12-13 0.2849722050994288 1106 14-15 0.34043913381434976 1106 16-17 0.34406345932481486 1106 18-19 0.4491943381187795 1106 20-21 0.4379627032068285 1106 22-23 0.5152288575374016 1106 24-25 0.45953435250186203 1106 26-27 0.4605544139094704 1106 28-29 0.3810519168673423 1106 30-31 0.386415724219475 1106 32-33 0.46536436639850365 1106 34-35 0.3958192129152778 1106 36-37 0.36150669682055536 1106 38-39 0.5486306836358281 1106 40-41 0.6282027818141245 1106 42-43 0.5781875502644667 1106 44-45 0.5480191657493094 1106 46-47 0.4980734852764783 1106 48-49 0.4310541948614386 1106 50-51 0.31382383546472425 1106 52-53 0.35546991437666264 1106 54-55 0.41857363618368026 1106 56-57 0.4285870399522551 1106 58-59 0.4660792336328896 1106 60-61 0.5670257591859951 1106 62-63 0.5819703468319375 1106 64-65 0.40220749122494937 1106 66-67 0.3696298157673965 1106 68-69 0.46107168813848887 1106 70-71 0.5122949882335845 1106 72-73 0.5428194259123167 1106 74-75 0.5635293176537779 1106 76-77 0.46073921107847227 1106 78-79 0.49566417070516167 1106 80-81 0.5114578918952972 1106 82-83 0.5905512703713995 1106 84-85 0.6938359519192652 1106 86-87 0.8336075751935397 1106 88-89 0.7835772335841931 1106 90-91 0.729829111621374 1106 92-93 0.8387990773011289 1106 94-95 0.722694958022366 1106 96-97 0.9134067890792714 1106 98-99 0.8820338282300675 1106 100-101 1.052992499648763 1107 1 0.09061434311942307 1107 2 0.19843160520738223 1107 3 0.23244748640135526 1107 4 0.23913637509698304 1107 5 0.17015142471639422 1107 6 0.22938073918786728 1107 7 0.14769053046514102 1107 8 0.2091643979978528 1107 9 0.17290800926996042 1107 10-11 0.18955201018498968 1107 12-13 0.341257904258093 1107 14-15 0.35231166280770054 1107 16-17 0.41166879557051317 1107 18-19 0.38416328136417377 1107 20-21 0.36748660771438324 1107 22-23 0.28231294553715003 1107 24-25 0.28448093586895595 1107 26-27 0.27650102313324965 1107 28-29 0.3105975738262501 1107 30-31 0.3813931652857292 1107 32-33 0.3512959992647353 1107 34-35 0.4006678548440661 1107 36-37 0.4188301520784279 1107 38-39 0.5468428270854488 1107 40-41 0.35734320702118794 1107 42-43 0.39304725516167593 1107 44-45 0.1791529979115012 1107 46-47 0.302012638744948 1107 48-49 0.5174512378414065 1107 50-51 0.5424576953305049 1107 52-53 0.6126127216823676 1107 54-55 0.6111320815318777 1107 56-57 0.5091684829770458 1107 58-59 0.5190031016948282 1107 60-61 0.4665999241688965 1107 62-63 0.5409371690081102 1107 64-65 0.5580921244314254 1107 66-67 0.4603946381134918 1107 68-69 0.5973015963636641 1107 70-71 0.5196673762464172 1107 72-73 0.6107454569066881 1107 74-75 0.6786360565221621 1107 76-77 0.7240050256236401 1107 78-79 0.7315797662688119 1107 80-81 0.7823250595250215 1107 82-83 0.7228546559147198 1107 84-85 0.7601505059098734 1107 86-87 0.8554385713696213 1107 88-89 0.7912368668214462 1107 90-91 0.8994786857863595 1107 92-93 0.7540116644186732 1107 94-95 0.7713423146685798 1107 96-97 0.8056945497957422 1107 98-99 0.9350769243508701 1107 100-101 1.0677605672143393 1108 1 0.2913270327024584 1108 2 0.27100327829253246 1108 3 0.28620016077020694 1108 4 0.25375007737435595 1108 5 0.2485577386040987 1108 6 0.2640296242732063 1108 7 0.30632030272777655 1108 8 0.32638543161169764 1108 9 0.4705374487222471 1108 10-11 0.46093864071796276 1108 12-13 0.46735546523370886 1108 14-15 0.5705373082220504 1108 16-17 0.5212720380941676 1108 18-19 0.5187049539738808 1108 20-21 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13775.0 11 16788.0 12 20902.0 13 27812.0 14 24396.0 15 24051.0 16 28100.0 17 34028.0 18 32157.0 19 27558.0 20 29508.0 21 31540.0 22 35067.0 23 39056.0 24 44383.0 25 50700.0 26 58604.0 27 68085.0 28 79708.0 29 93797.0 30 111880.0 31 137233.0 32 170187.0 33 216403.0 34 288951.0 35 409299.0 36 618275.0 37 883787.0 38 712160.0 39 99498.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19380399660889 18.30113748538278 13.060736162887407 24.444322355120917 2 15.851494563885376 19.91708354388864 39.01168682204534 25.219735070180644 3 19.991871094454183 22.857956511692322 29.218268021692236 27.93190437216126 4 13.115454407900028 15.482018069396233 36.13023531931194 35.27229220339179 5 14.267142923048432 36.65378557631383 34.3630702356328 14.716001265004941 6 33.900861728129414 34.78960865510315 16.706214791199045 14.603314825568381 7 29.429431235493542 31.5319041827602 20.597899932730133 18.440764649016124 8 26.512954562413892 34.83546040680767 19.627406670683587 19.024178360094844 9 26.037778635143333 16.389542555874048 20.15896359354576 37.41371521543685 10-11 25.742944553573928 25.71492246423759 27.028073656217742 21.51405932597074 12-13 26.11387169648589 23.00750035750991 27.514386154364985 23.364241791639213 14-15 22.869009045952343 24.66141578292209 25.601115410929115 26.86845976019645 16-17 22.937256788753977 27.54259468393397 26.29149487349117 23.22865365382088 18-19 22.75308046699843 26.9279156187003 27.697443435433968 22.621560478867305 20-21 23.50478863274466 26.054352407370608 27.630094605347693 22.81076435453704 22-23 23.056163838609994 25.886262871188357 27.734736576148876 23.322836714052777 24-25 23.15325889263661 25.994368205708113 27.85762868594252 22.99474421571276 26-27 23.065842599043748 25.949284592759188 28.006950758921896 22.97792204927517 28-29 22.847049780258345 26.58577722819155 27.781216405149028 22.78595658640108 30-31 23.040963410909153 26.187192113836588 27.506227624608748 23.26561685064551 32-33 22.921144438206753 26.17396699369993 27.78706748291318 23.117821085180136 34-35 23.383046300704905 26.172502753601567 27.599026926784127 22.845424018909405 36-37 23.292964600621595 26.03164663443253 27.796839050372768 22.878549714573104 38-39 22.918170797715064 26.425860318227162 27.80688746639469 22.849081417663083 40-41 23.09972886093641 26.330577218728163 27.649923130677852 22.91977078965758 42-43 22.954725093511144 26.30077040230679 27.38731491962845 23.357189584553613 44-45 23.044341251407282 26.230526633106248 27.482638607307862 23.242493508178605 46-47 23.34171939318377 26.563013281724746 27.238849249007146 22.85641807608434 48-49 23.26974870245626 26.376459124924978 27.330972518753487 23.022819653865277 50-51 22.80977903145769 26.641748436019892 27.536833683618937 23.01163884890348 52-53 23.184658199176997 26.840690127777496 26.946658663187854 23.02799300985766 54-55 23.07152483765251 26.43419855648122 27.106339574666755 23.387937031199506 56-57 22.96231136840895 26.65667356208775 27.186399805147882 23.194615264355413 58-59 23.445522396069997 26.434527408753773 27.07492146437631 23.045028730799917 60-61 23.094634790122388 26.55599670196397 27.444626737272444 22.9047417706412 62-63 22.85322286674487 26.523915041118517 27.51470084277077 23.108161249365843 64-65 22.84940525753081 26.73974491971114 27.238161337549954 23.1726884852081 66-67 22.903196933521595 26.608133417767405 26.993703247056928 23.49496640165407 68-69 22.73536881723053 26.506992187736028 27.318810595174607 23.43882839985883 70-71 23.0404918069106 26.286733400722333 27.188685988377078 23.484088803989987 72-73 22.935012363259307 26.271140076997806 27.39519479195814 23.39865276778475 74-75 22.6605195937669 26.845439275651735 27.042999787089332 23.451041343492033 76-77 22.939356953378383 26.510203889884878 26.97387877564954 23.5765603810872 78-79 22.849469039354766 26.54531477750066 27.131933526979253 23.473282656165324 80-81 22.948844554144294 26.295628172681084 27.168204241537 23.587323031637624 82-83 22.95840148122183 26.32282587310853 27.305229205633946 23.413543440035692 84-85 22.848932794980023 26.367523332054894 27.163678219698305 23.61986565326678 86-87 22.710278398193964 26.398464188921583 27.344040718142004 23.54721669474245 88-89 22.761317572102264 26.31022317473669 27.024545641649738 23.903913611511307 90-91 22.797835586848937 26.17320898816773 27.131416455992735 23.897538968990602 92-93 22.884684792329963 26.244877921511844 27.196852275374216 23.67358501078397 94-95 22.860246874903847 26.00685297433072 27.347328319007353 23.78557183175808 96-97 22.577252046338476 25.883647684754457 27.61658569024179 23.922514578665275 98-99 22.597875777524656 26.035626423883894 27.206910251247997 24.15958754734346 100-101 22.911374508199785 25.171871410140856 27.740529930602676 24.176224151056687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2994.0 1 1719.0 2 534.5 3 646.5 4 595.5 5 616.5 6 1013.0 7 1825.5 8 2865.0 9 3515.5 10 3467.0 11 3334.5 12 3580.0 13 4007.0 14 4415.5 15 4933.5 16 5378.0 17 5617.0 18 5909.5 19 6321.0 20 7014.5 21 8101.0 22 9875.0 23 12517.5 24 14896.5 25 17788.0 26 22514.0 27 28450.5 28 33388.0 29 38035.5 30 43460.5 31 50679.0 32 58377.0 33 64859.5 34 72710.0 35 80403.5 36 89211.0 37 97488.5 38 103260.0 39 109165.0 40 115262.0 41 120951.0 42 126892.0 43 131749.0 44 137217.0 45 145114.0 46 153670.5 47 167424.5 48 187224.5 49 190217.0 50 175664.0 51 168850.0 52 170309.0 53 172052.5 54 171328.5 55 163403.0 56 150198.5 57 137422.0 58 127918.5 59 116037.5 60 99466.5 61 82993.0 62 66158.5 63 51871.0 64 38479.0 65 26875.5 66 19517.0 67 14706.5 68 10997.0 69 8497.0 70 6480.0 71 4273.5 72 2906.0 73 2060.0 74 1334.5 75 803.5 76 541.5 77 325.5 78 202.0 79 132.0 80 90.0 81 60.0 82 47.5 83 39.5 84 34.5 85 36.0 86 29.0 87 22.0 88 18.5 89 15.5 90 12.0 91 10.5 92 11.0 93 10.0 94 9.0 95 6.5 96 6.0 97 6.0 98 4.5 99 3.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3202817107722803 2 0.0857305114467263 3 0.09559252561730532 4 0.10031204481406096 5 0.1122889378699786 6 0.056010897636967906 7 0.057947681458278 8 0.05872684736340276 9 0.061865772866905325 10-11 0.0593835729120079 12-13 0.05781411016025662 14-15 0.05602202857846968 16-17 0.05727982496817108 18-19 0.0652607100249489 20-21 0.06720862478776078 22-23 0.08264724065073265 24-25 0.0906169947660087 26-27 0.0854856307336871 28-29 0.08366015632739483 30-31 0.07822825687452513 32-33 0.06996909828020276 34-35 0.316363619363653 36-37 0.4939243982000822 38-39 0.4666758534037195 40-41 0.447641943435672 42-43 0.4792204244762279 44-45 0.4883255346246858 46-47 0.4823593499797305 48-49 0.5004582608616284 50-51 0.4963732053304743 52-53 0.49554951565934247 54-55 0.4867449409314327 56-57 0.46527335477449494 58-59 0.4480649192127397 60-61 0.4098746589201251 62-63 0.4784301276296014 64-65 0.5015045693627959 66-67 0.5123127135610264 68-69 0.523065203051748 70-71 0.5209280622834058 72-73 0.509674680425104 74-75 0.511411107299382 76-77 0.485865596552792 78-79 0.4660747825626232 80-81 0.4719073959095571 82-83 0.4812907795955595 84-85 0.48273780199079114 86-87 0.48178054102163786 88-89 0.48849249874721257 90-91 0.5030295083485401 92-93 0.5086840266314454 94-95 0.5147058659839095 96-97 0.5071479567041994 98-99 0.5100753943191682 100-101 0.48811404673615194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4491983.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.256823684602956 #Duplication Level Percentage of deduplicated Percentage of total 1 70.16547794447115 33.157976199677705 2 14.81751679308008 14.004575570684574 3 5.878989527854763 8.334671146843792 4 2.9053008968893543 5.4918116894007625 5 1.6232363175626336 3.835449622875078 6 1.0332114206683438 2.9295773961265237 7 0.7047214363827871 2.3312027666131057 8 0.5088405056848998 1.923694884858841 9 0.3711058894815217 1.5783557028791422 >10 1.808826229513899 14.885139415478266 >50 0.11823155639411477 3.8344181676589573 >100 0.06119142076416466 5.073757046778819 >500 0.002028910661025907 0.6353044488384063 >1k 0.001085230842958748 1.0786258397161708 >5k 1.8873579877537613E-4 0.6273439864474696 >10k+ 4.718394969384403E-5 0.27809611512254634 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12472 0.27765020482045455 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9158 0.2038743245466423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7979 0.17762756448543995 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5963 0.13274760835025423 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5035 0.11208858092294649 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4856 0.10810370386530849 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4596 0.10231561428438177 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.226188300356435E-5 2 0.0 0.0 0.0 0.0 2.226188300356435E-5 3 0.0 0.0 0.0 0.0 2.226188300356435E-5 4 0.0 0.0 0.0 0.0 2.226188300356435E-5 5 0.0 0.0 0.0 0.0 2.226188300356435E-5 6 0.0 0.0 0.0 0.0 2.226188300356435E-5 7 0.0 0.0 0.0 0.0 2.226188300356435E-5 8 0.0 0.0 0.0 0.0 2.226188300356435E-5 9 0.0 0.0 0.0 0.0 4.45237660071287E-5 10-11 0.0 0.0 0.0 0.0 4.45237660071287E-5 12-13 0.0 0.0 0.0 2.226188300356435E-5 4.45237660071287E-5 14-15 0.0 0.0 0.0 2.226188300356435E-5 5.565470750891088E-5 16-17 0.0 0.0 0.0 2.226188300356435E-5 1.1130941501782175E-4 18-19 0.0 0.0 0.0 2.226188300356435E-5 1.4470223952316828E-4 20-21 0.0 0.0 0.0 4.45237660071287E-5 1.5583318102495045E-4 22-23 0.0 0.0 0.0 1.4470223952316828E-4 1.8922600553029697E-4 24-25 0.0 1.1130941501782175E-5 0.0 2.8940447904633656E-4 2.226188300356435E-4 26-27 0.0 2.226188300356435E-5 0.0 5.120233090819801E-4 2.226188300356435E-4 28-29 0.0 2.226188300356435E-5 0.0 0.0021148788853386133 2.226188300356435E-4 30-31 0.0 2.226188300356435E-5 0.0 0.007324159508172671 2.226188300356435E-4 32-33 0.0 2.226188300356435E-5 0.0 0.017831768285855046 2.5601165454099004E-4 34-35 2.226188300356435E-5 2.226188300356435E-5 0.0 0.03450591865552474 2.8940447904633656E-4 36-37 4.45237660071287E-5 2.226188300356435E-5 0.0 0.06350202126766731 2.8940447904633656E-4 38-39 4.45237660071287E-5 2.226188300356435E-5 0.0 0.10599995592147166 2.8940447904633656E-4 40-41 4.45237660071287E-5 2.226188300356435E-5 0.0 0.15183717302581065 2.8940447904633656E-4 42-43 4.45237660071287E-5 2.226188300356435E-5 0.0 0.2010693272881932 3.0053542054811873E-4 44-45 4.45237660071287E-5 2.226188300356435E-5 0.0 0.26643021578665815 3.116663620499009E-4 46-47 4.45237660071287E-5 2.226188300356435E-5 0.0 0.3438347829900514 3.227973035516831E-4 48-49 4.45237660071287E-5 2.226188300356435E-5 0.0 0.4274949393174462 3.3392824505346525E-4 50-51 4.45237660071287E-5 2.226188300356435E-5 0.0 0.516932054284266 3.3392824505346525E-4 52-53 4.45237660071287E-5 2.226188300356435E-5 0.0 0.6097974992336347 3.450591865552474E-4 54-55 4.45237660071287E-5 2.226188300356435E-5 0.0 0.7181460838119824 3.561901280570296E-4 56-57 4.45237660071287E-5 2.226188300356435E-5 0.0 0.8513277988808061 3.6732106955881177E-4 58-59 4.45237660071287E-5 4.45237660071287E-5 0.0 0.9949614680198032 4.007138940641583E-4 60-61 4.45237660071287E-5 4.45237660071287E-5 0.0 1.1560707153165986 4.007138940641583E-4 62-63 4.45237660071287E-5 5.565470750891088E-5 0.0 1.3215432916820924 4.45237660071287E-4 64-65 4.45237660071287E-5 6.678564901069305E-5 0.0 1.4961988947865565 4.45237660071287E-4 66-67 4.45237660071287E-5 6.678564901069305E-5 0.0 1.701175182541875 4.45237660071287E-4 68-69 4.45237660071287E-5 6.678564901069305E-5 0.0 1.9412695907353166 4.45237660071287E-4 70-71 4.45237660071287E-5 6.678564901069305E-5 0.0 2.200030587827247 4.45237660071287E-4 72-73 4.45237660071287E-5 6.678564901069305E-5 0.0 2.4644349722605807 5.565470750891088E-4 74-75 4.45237660071287E-5 6.678564901069305E-5 0.0 2.745468983297577 5.565470750891088E-4 76-77 4.45237660071287E-5 6.678564901069305E-5 0.0 3.054363740913534 5.565470750891088E-4 78-79 4.45237660071287E-5 6.678564901069305E-5 0.0 3.4148170195657466 5.565470750891088E-4 80-81 4.45237660071287E-5 6.678564901069305E-5 0.0 3.8028193784348696 5.565470750891088E-4 82-83 4.45237660071287E-5 6.678564901069305E-5 0.0 4.198880538951283 5.788089580926731E-4 84-85 4.45237660071287E-5 6.678564901069305E-5 0.0 4.598614910163285 5.788089580926731E-4 86-87 4.45237660071287E-5 6.678564901069305E-5 0.0 5.028102288009549 5.788089580926731E-4 88-89 4.45237660071287E-5 6.678564901069305E-5 0.0 5.503894382503228 5.788089580926731E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 11115 0.0 24.291174 2 GTACATG 11845 0.0 23.762077 1 ACATGGG 11540 0.0 22.575634 3 GAGTACT 10120 0.0 19.580725 12-13 CATGGGG 8190 0.0 18.68107 4 AGAGTAC 14375 0.0 18.642761 10-11 GTACTTT 10610 0.0 18.610006 14-15 TACCGTA 285 4.4412445E-8 18.274363 7 AGTACTT 10580 0.0 17.901445 12-13 AGTACAT 8385 0.0 17.512241 2 GAGTACA 9290 0.0 17.217852 1 GTGTAGC 2545 0.0 17.157928 1 GTACCGT 455 9.094947E-12 16.64957 6 GTATCAA 25230 0.0 16.442165 1 TAGCCCT 2975 0.0 16.240385 4 GTATAGG 1140 0.0 15.82135 1 CAACGCA 26940 0.0 15.103538 5 GTATAGC 1575 0.0 15.067953 1 TCAACGC 27285 0.0 14.895204 4 GGTATCA 21670 0.0 14.89412 1 >>END_MODULE