FastQCFastQC Report
Fri 27 May 2016
SRR939013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491983
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT131880.2935897130510067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90800.2021378976723643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85590.19053945662750726No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58500.13023201557085146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51850.11542786337348115No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46190.10282763759346375No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45580.10146966273024631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117200.023.5763741
TACATGG114450.023.344452
ACATGGG117300.021.8467483
AGAGTAC149700.018.75244310-11
GTACCGT4051.8189894E-1218.7480476
GAGTACT109550.018.58541512-13
CATGGGG84550.018.5782784
GTACTTT112600.017.87134414-15
GTGTACG4800.017.86521
TATTCCG2403.1216478E-617.7959985
GAGTACA93250.017.676811
AGTACAT83950.017.184782
AGTACTT115450.016.97787912-13
GTGTAGC24300.016.4683321
GTATCAA249450.016.2716921
GTATTAG10650.016.1038441
TATACTG22400.015.2537135
GTATAGC15550.015.012131
TATACAG28950.014.9170835
GTCTAGA24350.014.6736751
Produced by FastQC (version 0.11.5)