FastQCFastQC Report
Fri 27 May 2016
SRR939012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500857
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124780.2772360908155936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.20082842000978926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80980.17992129054533393No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59820.13290802173897104No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51120.11357836963049482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.10671301043334637No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47120.1046911732587816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116950.023.5137841
TACATGG112450.023.4434662
ACATGGG116150.021.5149733
GAGTACA91550.019.3690361
GAGTACT99050.019.18458212-13
AGTACAT82000.018.527522
AGAGTAC142450.018.29030610-11
CATGGGG79900.018.1851834
GTACTTT106050.017.58414614-15
GTGTAGC24900.017.5179481
GTATCAA231550.017.261461
AGTACTT106050.016.33396712-13
TAGACCG3905.7971192E-915.7764655
CAACGCA249050.015.6782775
TCAACGC250650.015.559144
ATCAACG251200.015.5062333
AACGCAG254600.015.4284266
TATCAAC257350.015.3195822
GGTATCA197600.015.1883831
GTATAGC17250.015.1170951
Produced by FastQC (version 0.11.5)