##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939012_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4500857 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.718700016463533 33.0 31.0 34.0 30.0 34.0 2 31.7829635556073 33.0 31.0 34.0 30.0 34.0 3 31.847327297890157 34.0 31.0 34.0 30.0 34.0 4 35.37837860656315 37.0 35.0 37.0 33.0 37.0 5 35.40254511529693 37.0 35.0 37.0 33.0 37.0 6 35.47272441670553 37.0 35.0 37.0 33.0 37.0 7 35.37409253393298 37.0 35.0 37.0 33.0 37.0 8 35.354015024249826 37.0 35.0 37.0 33.0 37.0 9 36.95576486877943 39.0 37.0 39.0 33.0 39.0 10-11 36.838866575854325 39.0 37.0 39.0 33.0 39.0 12-13 36.70640913497141 39.0 37.0 39.0 32.0 39.0 14-15 37.92114035171524 40.0 38.0 41.0 33.0 41.0 16-17 37.83177425987984 40.0 38.0 41.0 32.0 41.0 18-19 37.7391042417033 40.0 38.0 41.0 32.0 41.0 20-21 37.59417562033186 40.0 37.0 41.0 32.0 41.0 22-23 37.69035352600627 40.0 38.0 41.0 32.0 41.0 24-25 37.6264975759061 40.0 37.5 41.0 32.0 41.0 26-27 37.53599781108353 40.0 37.0 41.0 32.0 41.0 28-29 37.40781122350699 40.0 37.0 41.0 31.0 41.0 30-31 37.24202135282236 40.0 37.0 41.0 31.0 41.0 32-33 37.0666254226695 40.0 36.5 41.0 30.5 41.0 34-35 36.74719225694129 40.0 36.0 41.0 30.0 41.0 36-37 36.48576893244997 39.0 36.0 41.0 30.0 41.0 38-39 36.278105925160474 39.0 35.5 41.0 30.0 41.0 40-41 36.141333861529034 39.0 35.0 41.0 29.0 41.0 42-43 35.94000498127357 39.0 35.0 41.0 28.5 41.0 44-45 35.910860309492165 39.0 35.0 41.0 28.0 41.0 46-47 35.741052870597755 39.0 35.0 41.0 27.0 41.0 48-49 35.58403966177997 39.0 35.0 41.0 26.5 41.0 50-51 34.43291277638903 38.0 34.0 40.0 25.0 40.5 52-53 34.58608271713587 38.0 34.0 39.5 25.0 40.5 54-55 35.07437316937641 39.0 35.0 40.0 26.0 41.0 56-57 34.949712576960344 38.5 34.5 40.0 26.0 41.0 58-59 34.727571660241594 38.0 34.0 40.0 25.0 41.0 60-61 34.43751501103012 38.0 34.0 40.0 25.0 41.0 62-63 34.06556929047068 37.0 34.0 40.0 23.5 41.0 64-65 33.64000933599979 37.0 33.0 40.0 22.5 41.0 66-67 33.24208289665724 36.0 33.0 39.0 22.0 41.0 68-69 32.82963744460221 36.0 32.0 39.0 21.0 40.5 70-71 32.408484650812056 35.0 32.0 38.5 20.0 40.0 72-73 31.97159540949646 35.0 31.5 37.5 18.5 40.0 74-75 31.503524884260933 35.0 31.0 37.0 17.5 39.0 76-77 31.086874455242636 35.0 31.0 36.5 14.0 39.0 78-79 30.655306311664646 35.0 30.5 36.0 9.5 38.5 80-81 30.216304694861446 34.0 30.0 35.5 7.0 37.0 82-83 29.82778912993681 34.0 30.0 35.0 4.0 37.0 84-85 29.46899368275864 34.0 29.5 35.0 2.0 36.5 86-87 29.068951646319803 34.0 29.0 35.0 2.0 36.0 88-89 28.641992958229956 34.0 29.0 35.0 2.0 36.0 90-91 28.198423877941465 34.0 28.5 35.0 2.0 35.0 92-93 27.76272552093968 34.0 27.0 35.0 2.0 35.0 94-95 27.25897256900186 33.5 26.5 35.0 2.0 35.0 96-97 26.63710211188669 33.0 25.0 35.0 2.0 35.0 98-99 25.873378447704518 33.0 24.0 35.0 2.0 35.0 100-101 23.152756463935646 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3594943884647286 1101 2 0.44610736753394065 1101 3 0.4088019147602111 1101 4 0.3411025920763322 1101 5 0.15491937087153929 1101 6 0.30165972172726185 1101 7 0.2383739384704242 1101 8 0.32487070553624164 1101 9 0.3126072651078644 1101 10-11 0.41795395531698176 1101 12-13 0.5388899940319902 1101 14-15 0.3629797703862394 1101 16-17 0.5631174810884332 1101 18-19 0.5049307039662452 1101 20-21 0.6314389905127982 1101 22-23 0.5814967805315021 1101 24-25 0.6390715159318461 1101 26-27 0.5312979538403368 1101 28-29 0.5463281051544655 1101 30-31 0.5542340977564706 1101 32-33 0.4325146303195453 1101 34-35 0.4237531791501823 1101 36-37 0.4361012390038965 1101 38-39 0.4713912239650071 1101 40-41 0.49734915331109164 1101 42-43 0.32720330829368294 1101 44-45 0.4220268395358886 1101 46-47 0.48108128235691794 1101 48-49 0.5480488398097378 1101 50-51 0.441649439388101 1101 52-53 0.43706770341576373 1101 54-55 0.49788483677299666 1101 56-57 0.4695873383429614 1101 58-59 0.3982631179268026 1101 60-61 0.35106204965026677 1101 62-63 0.4095549688560425 1101 64-65 0.48718290214382165 1101 66-67 0.3883722402792529 1101 68-69 0.32736131956146153 1101 70-71 0.24439692372024524 1101 72-73 0.19268324461992137 1101 74-75 0.14309047631432392 1101 76-77 0.11974165576640416 1101 78-79 -0.01481251676462847 1101 80-81 -0.0199742797178466 1101 82-83 -0.037875128026716 1101 84-85 -0.15253605932494807 1101 86-87 0.002474338874431936 1101 88-89 -0.0726555179701478 1101 90-91 -0.038314301268254525 1101 92-93 -0.05975817971519959 1101 94-95 -0.14396677258881851 1101 96-97 -0.10440135018351171 1101 98-99 -0.1888372387485724 1101 100-101 0.13579183480091572 1102 1 0.17962820283738878 1102 2 0.10694181584164042 1102 3 0.133407517000677 1102 4 0.18948643450283953 1102 5 0.2225176250228813 1102 6 0.3122420453313026 1102 7 0.23866031121715992 1102 8 0.21559158513024101 1102 9 0.33610602468638007 1102 10-11 0.25540726843482986 1102 12-13 0.30891776542713956 1102 14-15 0.38381491445102967 1102 16-17 0.3616679126268636 1102 18-19 0.4827974222076321 1102 20-21 0.45871336646612804 1102 22-23 0.4776894406867669 1102 24-25 0.5114094135262164 1102 26-27 0.5026702212897973 1102 28-29 0.5059229117094546 1102 30-31 0.5376292222440995 1102 32-33 0.4184667166475151 1102 34-35 0.4107759734829628 1102 36-37 0.3724920285322568 1102 38-39 0.32283841507476296 1102 40-41 0.28339005226851555 1102 42-43 0.2624849214294116 1102 44-45 0.41947276759569263 1102 46-47 0.5168393255215662 1102 48-49 0.5480017835111042 1102 50-51 0.44998475657427406 1102 52-53 0.4107906320034971 1102 54-55 0.5236172437970055 1102 56-57 0.513403022910687 1102 58-59 0.49800281426045956 1102 60-61 0.43592873855644143 1102 62-63 0.40092319153582423 1102 64-65 0.521271973446602 1102 66-67 0.45498558374432463 1102 68-69 0.4583177718197149 1102 70-71 0.5202067037312332 1102 72-73 0.4677484663946814 1102 74-75 0.5287223508006171 1102 76-77 0.35540979577323384 1102 78-79 0.300803892939026 1102 80-81 0.19933271786456075 1102 82-83 0.3059412544791549 1102 84-85 0.32169982653904583 1102 86-87 0.34156431695154055 1102 88-89 0.31701515555216275 1102 90-91 0.4063047973511651 1102 92-93 0.32481192909881074 1102 94-95 0.34701232230972323 1102 96-97 0.32842294814055606 1102 98-99 0.28659138524346517 1102 100-101 0.3988295375287727 1103 1 0.1805142265922406 1103 2 0.2353467425509237 1103 3 0.29265965625462087 1103 4 0.3061142789051914 1103 5 0.2222798187075128 1103 6 0.25246135666967007 1103 7 0.2701415134730354 1103 8 0.20777601585556482 1103 9 0.3731474873353591 1103 10-11 0.3536086803282572 1103 12-13 0.4429551341810338 1103 14-15 0.3710533107892289 1103 16-17 0.40205997853954756 1103 18-19 0.43142040008506655 1103 20-21 0.49814099565536907 1103 22-23 0.5097623773706559 1103 24-25 0.4896872828779095 1103 26-27 0.4041482308648483 1103 28-29 0.3267027441934687 1103 30-31 0.4647043858883819 1103 32-33 0.45367454069994295 1103 34-35 0.47872393979235284 1103 36-37 0.41044123094238927 1103 38-39 0.43383801621698126 1103 40-41 0.4513018769388282 1103 42-43 0.4115685942179752 1103 44-45 0.48938105811970445 1103 46-47 0.45485349944120657 1103 48-49 0.36945510637297474 1103 50-51 0.21860266167821152 1103 52-53 0.29749301205104217 1103 54-55 0.41820472712957013 1103 56-57 0.5343119533080554 1103 58-59 0.40245639808881606 1103 60-61 0.38239129473256384 1103 62-63 0.41159045755000534 1103 64-65 0.2625467522254823 1103 66-67 0.2839566563658025 1103 68-69 0.28893813767879095 1103 70-71 0.21224420909270236 1103 72-73 0.21252695697926782 1103 74-75 0.11614869221999058 1103 76-77 0.15147443287764162 1103 78-79 0.10417425455920082 1103 80-81 0.07864788488498675 1103 82-83 0.1750578949466579 1103 84-85 0.11896799038705907 1103 86-87 0.15278571019166876 1103 88-89 0.10799872857012005 1103 90-91 0.09336596754330628 1103 92-93 0.10858212411549673 1103 94-95 0.2143704209118411 1103 96-97 0.06300569353263086 1103 98-99 -0.0685057813151495 1103 100-101 0.15246773078486342 1104 1 0.11291578992279128 1104 2 0.15325919364923735 1104 3 0.19491923033712766 1104 4 0.22837642055929308 1104 5 0.22728854046362557 1104 6 0.24036298471846607 1104 7 0.195695002617164 1104 8 0.18369442653403922 1104 9 0.17276963008546176 1104 10-11 0.1864326075988103 1104 12-13 0.32238979554060165 1104 14-15 0.32762646597659995 1104 16-17 0.1915178120476 1104 18-19 0.29727504622858447 1104 20-21 0.3123932978080859 1104 22-23 0.315585358913701 1104 24-25 0.36740583947527483 1104 26-27 0.3009357936199919 1104 28-29 0.3395901128044514 1104 30-31 0.4406154969195626 1104 32-33 0.4582162172691753 1104 34-35 0.2779614228676124 1104 36-37 0.2903565846555267 1104 38-39 0.3167729642464039 1104 40-41 0.3758125744397063 1104 42-43 0.3766599617344255 1104 44-45 0.48766450374153436 1104 46-47 0.49250238249201317 1104 48-49 0.40798370271479456 1104 50-51 0.38307461180490776 1104 52-53 0.322110755176773 1104 54-55 0.45479621924253877 1104 56-57 0.3892605032251808 1104 58-59 0.311368569208625 1104 60-61 0.30489517108330944 1104 62-63 0.3306057372457758 1104 64-65 0.3287762934431768 1104 66-67 0.41301143191407874 1104 68-69 0.2943734920816965 1104 70-71 0.26712491460678933 1104 72-73 0.3529440302618525 1104 74-75 0.379794708413673 1104 76-77 0.3042905389463826 1104 78-79 0.2589378853677964 1104 80-81 0.19329675360098975 1104 82-83 0.209248041131616 1104 84-85 0.254090597749137 1104 86-87 0.2828355122998154 1104 88-89 0.19230111212183587 1104 90-91 0.24172360244277158 1104 92-93 0.24757573972760483 1104 94-95 0.1686958665883651 1104 96-97 0.29677795606835744 1104 98-99 0.21343320389528841 1104 100-101 0.6191528810753475 1105 1 0.16248449730031922 1105 2 0.18156317923738996 1105 3 0.19889289272670396 1105 4 0.1957662841585659 1105 5 0.2284862568018866 1105 6 0.23004109864725564 1105 7 0.18508631672573728 1105 8 0.3057983596945206 1105 9 0.31550238275472964 1105 10-11 0.23031905459917112 1105 12-13 0.33485904594275695 1105 14-15 0.40244407110360925 1105 16-17 0.3611533898871997 1105 18-19 0.41261797475691253 1105 20-21 0.3195930168647152 1105 22-23 0.3665218428777095 1105 24-25 0.388244177571039 1105 26-27 0.3088662447674295 1105 28-29 0.3547708779442118 1105 30-31 0.40897084376588566 1105 32-33 0.3123148821184074 1105 34-35 0.3309917428108804 1105 36-37 0.42255453198082193 1105 38-39 0.3802787981877458 1105 40-41 0.46320324174303096 1105 42-43 0.2984728221519575 1105 44-45 0.27803148757882923 1105 46-47 0.39304782133909555 1105 48-49 0.4093740380488953 1105 50-51 0.2808690516161718 1105 52-53 0.3811587054173131 1105 54-55 0.384523721512565 1105 56-57 0.322027102322906 1105 58-59 0.2925450984957223 1105 60-61 0.37299185294334336 1105 62-63 0.29720285166784777 1105 64-65 0.36749608633259356 1105 66-67 0.20912209614797206 1105 68-69 0.21267110774407882 1105 70-71 0.11453144520068292 1105 72-73 0.2312359525013008 1105 74-75 0.2904030879837869 1105 76-77 0.2346930643631353 1105 78-79 0.2451645887881746 1105 80-81 0.09629652707897307 1105 82-83 0.03823046093604532 1105 84-85 0.16955053582757884 1105 86-87 0.25668223980951055 1105 88-89 0.22996539444915598 1105 90-91 0.2569494850777865 1105 92-93 0.3875657484608759 1105 94-95 0.4496826168416419 1105 96-97 0.3538907343865816 1105 98-99 0.3496398588452543 1105 100-101 0.7155744977862852 1106 1 0.18908758653346425 1106 2 0.19864977458959032 1106 3 0.23229753648532636 1106 4 0.14210510486923056 1106 5 0.1463079514837986 1106 6 0.12986746022205153 1106 7 0.13529608221628564 1106 8 0.21141046117952556 1106 9 0.24655802915316372 1106 10-11 0.22217317486418153 1106 12-13 0.30459820509314284 1106 14-15 0.2487709809038492 1106 16-17 0.40110251270627373 1106 18-19 0.37403974413985 1106 20-21 0.4507949308610648 1106 22-23 0.4242456486825503 1106 24-25 0.36815126539185883 1106 26-27 0.28383027948014217 1106 28-29 0.28674524683784597 1106 30-31 0.2751553039306671 1106 32-33 0.3903588224578911 1106 34-35 0.34488127487994547 1106 36-37 0.17676978599464377 1106 38-39 0.2436335576783577 1106 40-41 0.3479613157831949 1106 42-43 0.3086348105338601 1106 44-45 0.36712224123409953 1106 46-47 0.33210399998738893 1106 48-49 0.2651091262563696 1106 50-51 0.27907374475754665 1106 52-53 0.23061910477456848 1106 54-55 0.08702482059232608 1106 56-57 0.19961274561115516 1106 58-59 0.40548949779959287 1106 60-61 0.39157133127377364 1106 62-63 0.31007785829063295 1106 64-65 0.1621125317610037 1106 66-67 0.26935214759744497 1106 68-69 0.3525687446922561 1106 70-71 0.3040977556050706 1106 72-73 0.2542721891712958 1106 74-75 0.253877517746421 1106 76-77 0.2647195039554049 1106 78-79 0.30281503834921253 1106 80-81 0.37436423868367896 1106 82-83 0.48022379628621437 1106 84-85 0.4776678415108826 1106 86-87 0.6354330390942913 1106 88-89 0.5693801845239115 1106 90-91 0.4601229742627204 1106 92-93 0.5897842920295311 1106 94-95 0.6064822704747925 1106 96-97 0.5676380635317493 1106 98-99 0.6816439241259395 1106 100-101 1.2276567413083868 1107 1 -0.016751660953271852 1107 2 -0.025315700410313724 1107 3 0.017439857725268126 1107 4 0.039514926581098564 1107 5 0.010947338741360113 1107 6 0.0739865425543016 1107 7 -0.03840129788404312 1107 8 0.14693052512065918 1107 9 0.11068994389296449 1107 10-11 0.09860120694720109 1107 12-13 0.06820407145134766 1107 14-15 0.03836608731844393 1107 16-17 0.1566187409825588 1107 18-19 0.14691140199050778 1107 20-21 0.16548994530003114 1107 22-23 0.09375879035312806 1107 24-25 0.14229662504066454 1107 26-27 0.1221855222917867 1107 28-29 0.1148736733316511 1107 30-31 0.13403014879710184 1107 32-33 0.16104073739850833 1107 34-35 -0.015505896327262292 1107 36-37 -0.009228882567768437 1107 38-39 0.060550847891583714 1107 40-41 0.045509351962948585 1107 42-43 0.027867958711020435 1107 44-45 0.261629860350574 1107 46-47 0.19448175072138696 1107 48-49 0.025136929806059527 1107 50-51 0.11505122679418633 1107 52-53 0.10038573167884834 1107 54-55 0.09430384814083936 1107 56-57 0.18947900943260976 1107 58-59 0.16240544251782296 1107 60-61 0.04808719204918788 1107 62-63 -0.005817257361684369 1107 64-65 0.1817760877235557 1107 66-67 0.10057410808406786 1107 68-69 0.06268295111126321 1107 70-71 0.17910483451534276 1107 72-73 0.0876267090304026 1107 74-75 0.11691320549518736 1107 76-77 0.21146938045101393 1107 78-79 0.22382760366596344 1107 80-81 0.1300070338591759 1107 82-83 0.17956736542256735 1107 84-85 0.19046239446515045 1107 86-87 0.22011322296522096 1107 88-89 0.3292074189493057 1107 90-91 0.27455802701817333 1107 92-93 0.3300208180016 1107 94-95 0.24871113314995696 1107 96-97 0.3894638500438674 1107 98-99 0.4828904649988637 1107 100-101 0.7099684021311852 1108 1 0.1809648007937703 1108 2 0.20983533870457904 1108 3 0.18785703312661894 1108 4 0.17138025252125288 1108 5 0.17184566698086456 1108 6 0.19255684903940562 1108 7 0.14498401063600141 1108 8 0.20511629299247858 1108 9 0.2522454666129903 1108 10-11 0.31991699573871557 1108 12-13 0.29274802483180906 1108 14-15 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16215.0 3 4676.0 4 4656.0 5 5305.0 6 5993.0 7 7436.0 8 8905.0 9 10798.0 10 13111.0 11 15886.0 12 19958.0 13 27458.0 14 25542.0 15 22914.0 16 26566.0 17 32004.0 18 29894.0 19 25577.0 20 26710.0 21 29234.0 22 32629.0 23 36729.0 24 41372.0 25 47558.0 26 55524.0 27 64449.0 28 75803.0 29 90312.0 30 107771.0 31 131362.0 32 162268.0 33 208159.0 34 280421.0 35 399032.0 36 612576.0 37 904288.0 38 772011.0 39 119701.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.194471404724155 18.33991966282271 13.049889440571969 24.41571949188117 2 15.84707898889377 19.930303655644643 39.000265958866215 25.22235139659537 3 20.028829691112676 22.84793049562651 29.211255321339642 27.91198449192117 4 13.096798974931781 15.510673605138331 36.106453361375465 35.28607405855442 5 14.2462061866493 36.63375299488168 34.41956457334113 14.700476245127897 6 33.893249474884044 34.74773309566916 16.708271237788246 14.65074619165854 7 29.437298559849562 31.57153839841501 20.600095670519803 18.391067371215627 8 26.519238236035818 34.85660774955491 19.61012056059096 19.014033453818318 9 26.027329934030274 16.424802873632746 20.154658800589246 37.393208391747734 10-11 25.761279810003185 25.682270132818758 27.054754729211734 21.501695327966324 12-13 26.127205191162712 23.011611354856733 27.48429466017334 23.37688879380722 14-15 22.87111968701509 24.62834598701738 25.609693173460336 26.890841152507193 16-17 22.93852538352906 27.508206626299653 26.323859497709883 23.229408492461403 18-19 22.73246936598915 26.91815284417885 27.715977657880543 22.633400131951458 20-21 23.52845018366885 26.013409110192747 27.647118284560886 22.811022421577523 22-23 23.097091142769628 25.892239288223834 27.672302517770937 23.338367051235593 24-25 23.144633954453603 26.02095756730533 27.827935140036537 23.00647333820453 26-27 23.103145258530553 25.9799400225138 27.95157752357053 22.965337195385118 28-29 22.846902804283424 26.58467940257683 27.77700537864155 22.791412414498197 30-31 23.033750915183013 26.19986429210851 27.53439937024065 23.23198542246783 32-33 22.91336159665173 26.168814552083564 27.79163659782779 23.12618725343691 34-35 23.35191396571716 26.17234066232338 27.63615370427243 22.839591667687028 36-37 23.27220590734688 26.0067208024289 27.824519855285857 22.89655343493836 38-39 22.89224830459838 26.420524847475026 27.838427032848017 22.84879981507858 40-41 23.099775322759942 26.318317549639957 27.644581110627907 22.93732601697219 42-43 22.97746930402269 26.30603894682857 27.35608026280468 23.360411486344056 44-45 23.06439417598376 26.242565590240375 27.471580551408646 23.22145968236722 46-47 23.337756412039667 26.580267482863785 27.24296957840206 22.83900652669449 48-49 23.264532831990802 26.386246068175613 27.32856530482618 23.020655795007407 50-51 22.8208018777322 26.67641691134462 27.541837723345804 22.960943487577374 52-53 23.199820766502555 26.809856054494137 26.961232375558986 23.029090803444323 54-55 23.054793681808448 26.46771743419891 27.092745645997677 23.384743237994957 56-57 23.00141242543438 26.665061933933785 27.135378939390808 23.198146701241022 58-59 23.439694223192006 26.406935980521816 27.107222146457854 23.046147649828324 60-61 23.094819707356216 26.51062704422589 27.483293150713685 22.911260097704215 62-63 22.870102845850987 26.5170986338387 27.526263352320274 23.086535167990046 64-65 22.851135468060672 26.752073194227822 27.236347494665864 23.16044384304564 66-67 22.923825802923268 26.58588711331443 26.95699885356952 23.533288230192777 68-69 22.727186865233758 26.52388327806714 27.26177554425381 23.487154312445295 70-71 23.03088368743071 26.300228869475102 27.182991970176438 23.485895472917743 72-73 22.91070780168048 26.247191629051287 27.414807858420666 23.427292710847567 74-75 22.65521957482209 26.832725641496165 27.041307026568646 23.4707477571131 76-77 22.923896117594897 26.515949934208642 26.99665813881976 23.563495809376697 78-79 22.841164667988657 26.501968851164815 27.141168231570724 23.515698249275804 80-81 22.925293566874668 26.304610721868105 27.180792870169277 23.589302841087946 82-83 22.96799172907584 26.29349938341217 27.29641820452394 23.442090682988052 84-85 22.816492058731907 26.383470488569998 27.159775981021582 23.640261471676506 86-87 22.71222077977213 26.430327053834173 27.32323965848939 23.534212507904304 88-89 22.77367891186563 26.343346037769162 27.038056321881466 23.844918728483748 90-91 22.783257342094675 26.187161950739796 27.174366367171785 23.85521433999374 92-93 22.84944824520091 26.26394865950038 27.22286137318536 23.663741722113354 94-95 22.876138540796916 26.018717337448187 27.357892439292257 23.747251682462636 96-97 22.52516733052012 25.92879600366964 27.636628438795714 23.909408227014524 98-99 22.57905922989414 26.075539358089024 27.224972610056277 24.120428801960564 100-101 22.79889417596511 25.401549503413857 27.630550098517205 24.16900622210383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4266.0 1 2419.0 2 626.5 3 700.5 4 619.0 5 627.0 6 1060.0 7 1864.0 8 2834.0 9 3478.0 10 3469.0 11 3379.5 12 3684.5 13 4139.0 14 4575.0 15 5003.0 16 5518.0 17 5893.0 18 6100.5 19 6445.5 20 7114.5 21 8184.5 22 9916.0 23 12553.0 24 15119.0 25 18040.0 26 22678.5 27 28340.5 28 33179.0 29 37956.0 30 43553.0 31 50823.0 32 58380.5 33 64861.0 34 72606.5 35 80486.0 36 89147.0 37 97671.0 38 103285.0 39 108821.0 40 115350.0 41 121617.5 42 127436.0 43 132030.0 44 137913.0 45 145249.0 46 153645.0 47 168053.0 48 188130.0 49 191193.5 50 176128.0 51 168961.0 52 171083.0 53 172764.0 54 171337.5 55 163113.5 56 150388.0 57 137802.5 58 127864.5 59 116071.5 60 99771.5 61 83155.5 62 65974.5 63 51795.0 64 38352.5 65 26522.5 66 19040.5 67 14515.0 68 11011.5 69 8370.5 70 6355.5 71 4176.5 72 2862.0 73 1980.0 74 1287.0 75 793.5 76 491.0 77 277.0 78 182.0 79 121.5 80 79.0 81 55.0 82 42.5 83 40.0 84 35.0 85 26.5 86 21.0 87 17.0 88 14.0 89 13.5 90 12.0 91 12.5 92 8.5 93 5.0 94 5.5 95 7.0 96 7.0 97 3.5 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28379039813973206 2 0.08711674243371872 3 0.09118263477377753 4 0.1058909447689629 5 0.1078239099798105 6 0.08342855593945776 7 0.09273789413882734 8 0.08956072143593986 9 0.09440424345852357 10-11 0.08752777526591046 12-13 0.09193804646537315 14-15 0.10082524283708635 16-17 0.08961626641326308 18-19 0.10012537612281394 20-21 0.10188059740622732 22-23 0.11495588506811036 24-25 0.12723132505653925 26-27 0.12968641305422499 28-29 0.1218434622561881 30-31 0.11490034009078716 32-33 0.10939027834032497 34-35 0.3811718523827795 36-37 0.5830556269617098 38-39 0.5652812342182834 40-41 0.528077208407199 42-43 0.555127612363601 44-45 0.5770567694108033 46-47 0.5658366839915153 48-49 0.5881324378890509 50-51 0.5798340182769637 52-53 0.5831556079208915 54-55 0.5727575881659871 56-57 0.5446629386359086 58-59 0.5389751329580121 60-61 0.48725165007464133 62-63 0.5626484022931633 64-65 0.5963975305147442 66-67 0.6089173684033952 68-69 0.6111613854872527 70-71 0.6075842889476382 72-73 0.5988970544942885 74-75 0.5959753886870878 76-77 0.5800006532089333 78-79 0.5559718960189137 80-81 0.5507839951369262 82-83 0.5647813294223745 84-85 0.5749793872589153 86-87 0.5653812151774651 88-89 0.5786009197803885 90-91 0.5922649842018975 92-93 0.600774474727813 94-95 0.5995191582403084 96-97 0.5966086014285723 98-99 0.6008522376960654 100-101 0.5777566361250758 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4500857.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.412491001129034 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46766826367657 33.41047687422112 2 14.600847141894551 13.84525067447872 3 5.848922997852283 8.319360270059043 4 2.88242859566737 5.466524794139048 5 1.62734099815208 3.8578145215326924 6 1.0403043900975801 2.9594053520361934 7 0.6922852220280223 2.2976076801732757 8 0.489678620055706 1.8573506533469155 9 0.37797862873868515 1.6128817500322805 >10 1.7917134389589127 14.88603948438518 >50 0.11759388308660398 3.83980057551371 >100 0.060048121345788324 5.064853159239974 >500 0.0018762934447562078 0.6007896070769937 >1k 0.0010788683937277572 1.076479310934964 >5k 1.8762928586567085E-4 0.6278554516662773 >10k+ 4.690732146641771E-5 0.27750984116367855 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12478 0.2772360908155936 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9039 0.20082842000978926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8098 0.17992129054533393 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5982 0.13290802173897104 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5112 0.11357836963049482 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4803 0.10671301043334637 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4712 0.1046911732587816 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2217990929283024E-5 2 0.0 0.0 0.0 0.0 2.2217990929283024E-5 3 0.0 0.0 0.0 0.0 2.2217990929283024E-5 4 0.0 0.0 0.0 0.0 2.2217990929283024E-5 5 0.0 0.0 0.0 0.0 2.2217990929283024E-5 6 0.0 0.0 0.0 0.0 2.2217990929283024E-5 7 0.0 0.0 0.0 0.0 2.2217990929283024E-5 8 0.0 0.0 0.0 0.0 2.2217990929283024E-5 9 0.0 0.0 0.0 0.0 4.443598185856605E-5 10-11 2.2217990929283024E-5 0.0 0.0 0.0 4.443598185856605E-5 12-13 2.2217990929283024E-5 2.2217990929283024E-5 0.0 0.0 6.665397278784907E-5 14-15 2.2217990929283024E-5 2.2217990929283024E-5 0.0 3.3326986393924534E-5 8.88719637171321E-5 16-17 2.2217990929283024E-5 2.2217990929283024E-5 0.0 5.554497732320756E-5 1.1108995464641512E-4 18-19 2.2217990929283024E-5 2.2217990929283024E-5 0.0 6.665397278784907E-5 1.1108995464641512E-4 20-21 2.2217990929283024E-5 2.2217990929283024E-5 0.0 1.3330794557569813E-4 1.1108995464641512E-4 22-23 2.2217990929283024E-5 2.2217990929283024E-5 0.0 2.999428775453208E-4 1.1108995464641512E-4 24-25 2.2217990929283024E-5 2.2217990929283024E-5 0.0 4.99904795908868E-4 1.1108995464641512E-4 26-27 2.2217990929283024E-5 2.2217990929283024E-5 0.0 7.665206870602643E-4 1.1108995464641512E-4 28-29 2.2217990929283024E-5 2.2217990929283024E-5 0.0 0.0028550118344128684 1.1108995464641512E-4 30-31 2.2217990929283024E-5 2.2217990929283024E-5 0.0 0.00925379322204638 1.2219895011105663E-4 32-33 2.2217990929283024E-5 2.2217990929283024E-5 0.0 0.020607186586910006 1.3330794557569813E-4 34-35 2.2217990929283024E-5 3.3326986393924534E-5 0.0 0.039148100017396686 1.3330794557569813E-4 36-37 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.06943122165400945 1.3330794557569813E-4 38-39 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.11614454758282701 1.3330794557569813E-4 40-41 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.16496858264992642 1.5552593650498117E-4 42-43 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.21539231306393428 1.5552593650498117E-4 44-45 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.28044659050487497 1.5552593650498117E-4 46-47 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.36457501315860513 1.777439274342642E-4 48-49 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.45203613445172774 1.777439274342642E-4 50-51 2.2217990929283024E-5 4.443598185856605E-5 0.0 0.547751239375079 1.777439274342642E-4 52-53 4.443598185856605E-5 4.443598185856605E-5 0.0 0.6466435170013178 2.3328890475747175E-4 54-55 4.443598185856605E-5 4.443598185856605E-5 0.0 0.7588776981805909 2.888338820806793E-4 56-57 4.443598185856605E-5 4.443598185856605E-5 0.0 0.8973846536337413 2.888338820806793E-4 58-59 4.443598185856605E-5 4.443598185856605E-5 0.0 1.0479115421796337 2.888338820806793E-4 60-61 4.443598185856605E-5 5.554497732320756E-5 0.0 1.210036221990612 2.888338820806793E-4 62-63 4.443598185856605E-5 6.665397278784907E-5 0.0 1.3781375413615673 3.1105187300996235E-4 64-65 4.443598185856605E-5 6.665397278784907E-5 0.0 1.5568479514012554 3.1105187300996235E-4 66-67 4.443598185856605E-5 6.665397278784907E-5 0.0 1.7668190746784447 3.1105187300996235E-4 68-69 4.443598185856605E-5 6.665397278784907E-5 0.0 2.0075621153926906 3.1105187300996235E-4 70-71 4.443598185856605E-5 8.88719637171321E-5 0.0 2.272311695306027 3.3326986393924536E-4 72-73 4.443598185856605E-5 1.1108995464641512E-4 0.0 2.5411827125367457 3.554878548685284E-4 74-75 4.443598185856605E-5 1.1108995464641512E-4 0.0 2.825517451454245 3.665968503331699E-4 76-77 6.665397278784907E-5 1.1108995464641512E-4 0.0 3.13540287994042 3.777058457978114E-4 78-79 6.665397278784907E-5 1.1108995464641512E-4 0.0 3.4946789022623914 3.777058457978114E-4 80-81 6.665397278784907E-5 1.1108995464641512E-4 0.0 3.889670345003185 3.888148412624529E-4 82-83 6.665397278784907E-5 1.1108995464641512E-4 0.0 4.288605481133926 4.110328321917359E-4 84-85 6.665397278784907E-5 1.1108995464641512E-4 0.0 4.696627775554744 4.221418276563774E-4 86-87 8.88719637171321E-5 1.1108995464641512E-4 0.0 5.1294231298617134 4.221418276563774E-4 88-89 8.88719637171321E-5 1.1108995464641512E-4 0.0 5.610053818639428 4.33250823121019E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11695 0.0 23.513784 1 TACATGG 11245 0.0 23.443466 2 ACATGGG 11615 0.0 21.514973 3 GAGTACA 9155 0.0 19.369036 1 GAGTACT 9905 0.0 19.184582 12-13 AGTACAT 8200 0.0 18.52752 2 AGAGTAC 14245 0.0 18.290306 10-11 CATGGGG 7990 0.0 18.185183 4 GTACTTT 10605 0.0 17.584146 14-15 GTGTAGC 2490 0.0 17.517948 1 GTATCAA 23155 0.0 17.26146 1 AGTACTT 10605 0.0 16.333967 12-13 TAGACCG 390 5.7971192E-9 15.776465 5 CAACGCA 24905 0.0 15.678277 5 TCAACGC 25065 0.0 15.55914 4 ATCAACG 25120 0.0 15.506233 3 AACGCAG 25460 0.0 15.428426 6 TATCAAC 25735 0.0 15.319582 2 GGTATCA 19760 0.0 15.188383 1 GTATAGC 1725 0.0 15.117095 1 >>END_MODULE